1. Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor
- Author
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Eleonora Macchia, Cinzia Di Franco, Lucia Sarcina, Kyriaki Manoli, Gaetano Scamarcio, Luisa Torsi, and Rosaria Anna Picca
- Subjects
Models, Molecular ,P24 capsid protein ,Biological organism ,Transistors, Electronic ,Computer science ,HIV-1 p24 detection ,Human immunodeficiency virus (HIV) ,HIV Core Protein p24 ,HIV Infections ,02 engineering and technology ,Computational biology ,Biosensing Techniques ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,law.invention ,law ,Disease spreading ,Limit of Detection ,medicine ,Humans ,Organic bioelectronics ,Detection limit ,Immunoassay ,biology ,Transistor ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,3. Good health ,Biomarker (cell) ,Electrolyte-gated thin-film transistors ,Hiv 1 p24 ,Single molecule detection with a transistor (SiMoT) ,biology.protein ,HIV-1 ,0210 nano-technology ,Antibodies, Immobilized ,Research Paper - Abstract
Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance. Herein, a label-free single molecule detection of HIV-1 p24 capsid protein with a large (wide-field) single-molecule transistor (SiMoT) sensor is proposed. The system is based on an electrolyte-gated field-effect transistor whose gate is bio-functionalized with the antibody against the HIV-1 p24 capsid protein. The device exhibits a limit of detection of a single protein and a limit of quantification in the 10 molecule range. This study paves the way for a low-cost technology that can quantify, with single-molecule precision, the transition of a biological organism from being “healthy” to being “diseased” by tracking a target biomarker. This can open to the possibility of performing the earliest possible diagnosis. Electronic supplementary material The online version of this article (10.1007/s00216-019-02319-7) contains supplementary material, which is available to authorized users. more...
- Published
- 2019