25 results on '"Herzfeld, T."'
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2. The Political Economy of Russian Agricultural Subsidies
- Author
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Kvartiuk, V., Herzfeld, T., and Ghukasyan, S.
- Subjects
Political Economy - Abstract
Agricultural policy s support to farmers, measured in real terms, differs considerably across Russian regions. What explains these large differences in regional agricultural support? We argue that traditional approaches of agricultural economics cannot fully explain this variation and we draw upon the political eocnomy literature. In particular, we explain allocation and distribution of agricultural subsidies studying the incentives of federal and regional politicians. Electoral pressures arising from competing with other political parties may push federal politicians to target either loyal or easily swayed voters and regional ones to strategically target special interst groups. Vertical organization of the Russian dominant party may generate perverse accountability links between local governors and regional agricultural interest groups. We utlize a unique dataset on the agricultural subsidies in 2008-2015 in order to test the hypotheses. The evidence suggests that federal government targets swing regions in distributing agricultural subsidies and local governments are more likely to allocate larger co-funding shares facing higher political competition in the region. In addition, regions with better organized large-scale agricultural producers and elected governors are more successful in maximizing obtained agricultural subsidies from the federal level. Acknowledgement
- Published
- 2018
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3. Agricultural labor adjustments and the impact of institutions: Panel data analysis
- Author
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Herzfeld, T., Glauben, T., Dries, L.K.E., and Teuber, R.
- Subjects
Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,WASS - Published
- 2015
4. Effects of Water Priority Policy on Farmer's Decision on Acreage Allocation in Northwest China
- Author
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Herzfeld, T. and Lei Zhang
- Subjects
Life Science ,WASS - Abstract
This article analyses the impact of a water allocation priority policy for a specific crop on farmers’ acreage allocation to different crops. To accomplish this, a system of crop acreage demands conditional on output yields, prices of variable inputs and levels of quasi-fixed inputs is estimated. The analysis based on a two-year farm household panel data from an arid region in northwest China. The results show that the water policy change results in a lower elasticity of land demand not only for Atlantic potatoes (i.e. the preferential crop), but also for the other crops. Acreage allocation to grains differs from other crops due to their use within the farm household. Moreover, the estimated elasticities of quasi-fixed inputs reveal that whereas the area of cash crops and Atlantic potatoes increases with increased use of own labour before the policy change, it does so only for cash crops after the policy change. With respect to own and exchanged labour Atlantic potatoes behave like grains and regular potatoes after the policy change.
- Published
- 2012
5. Farmgate private standards and price premium: evidence from the GlobalGAP scheme in Kenya's French beans marketing
- Author
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Kariuki, I.M., Loy, J.P., and Herzfeld, T.
- Subjects
industry ,WASS ,safety standards - Abstract
The proliferation of private quality and assurance schemes in international trade is defining market access in high value chains. The prime concern for small-scale producers is whether price premiums are realizable due to compliance. Using French beans marketing, the authors find that GlobalGAP certification, produce traceability, number of suppliers, competition for supplies, direct procurement, a good road network, and supply contracts have positive farmgate price effects for smallholders. Potential policy implications are drawn.
- Published
- 2012
6. Voluntary Certification Schemes and Legal Minimum Standards
- Author
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Herzfeld, T. and Jongeneel, R.A.
- Subjects
Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,WASS ,LEI INT BELEID - Landbouwbeleid - Abstract
EU farmers face increasing requests to comply with legal as well as private agribusiness and retail standards. Both requests potentially raise farmer’s administrative burden. This paper discusses the potential synergies between cross-compliance and third-party certification schemes. In selected aspects cross-compliance and several certification schemes ask similar measures. However, both regulatory approaches differ considerably in other areas. The heterogeneous nature of the various certification schemes in place prevent a general conclusion. As a tendency systemic standards like organic agriculture provide the largest overlap with cross-compliance. Certificates of origin, on the opposite side, have no relation with cross-compliance.
- Published
- 2012
7. Smallholders, local governance, and benefit distribution in forestry projects: The case of tea oil forestry in China
- Author
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Li, J., Bluemling, B., Herzfeld, T., and Feng, S.
- Subjects
Agribusiness ,Land Economics/Use - Abstract
While internationally, large-scale forestry projects have gained in importance for climate change mitigation, the implementation of such projects, particularly in regions with smallholder forestry like Southeast and East Asia, faces various obstacles. The organization of smallholders is one crucial element for the success of these projects. Hereby, an equal distribution of benefits is generally considered vital. It will support a balanced socio-economic development in poor rural areas, guaranteeing long-term success and a sustained stock of carbon sinks. China presents an especially interesting case of smallholder forestry. The conversion from collectively-managed to household-management forest in 2003, which is, however, still collectively owned, has increasedthe number of participants in large-scale forestry projects substantially. Simultaneously, the government has been promoting plantations for biodiesel production from oil seeds, such as jatropha and tea oil tree (camellia oleifera). Using the case of a governmental program for tea oil tree plantations, as well as the case of a European Investment bank project, this research inquires the distribution of benefits under their respective institutional arrangements and organization of smallholders. Empirical research was undertaken in three counties of Jiangxi province, China, in 2011, consisting of semi-structured stakeholder interviews and a survey with 308 smallholders. Results show that, on the village level, we can distinguish four implementation schemesof tea oil tree plantations, i.e. individual, partnership, collective, and cooperation with company. Distinguishing factors for these schemes are property rights relations, monetary input distribution, productive resource distribution and extension services. As this research will show, these criteria impact the distribution mechanisms of output sharing, respectively generating different distribution effects for poor smallholders. Results will be important also for other countries where smallholder forestry is prevalent, and carbon sink projects are to be implemented.
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- 2012
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8. Agricultural labour adjustment and the impact of Institutions
- Author
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Herzfeld, T., Glauben, T., and Dries, L.K.E.
- Subjects
Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,WASS - Abstract
The economic transformation in countries of Central and Eastern Europe as well as Asia resulted in a diverse picture of change in agricultural labour use. Based on a measure of sectoral labour adjustment, the paper explores the determinants of occupational labour flows paying special attention to the impact of institutions. Annual rates of occupational migration between agriculture and non-agriculture over the period 1978-2005 are calculated for a panel of 30 transition countries. Annual migration from agriculture ranges from outflows of nearly 8 percent of the agricultural labour force to immigration into agriculture about 9 percent on average. Fixed-effects panel models are used to explain the annual intersectoral labour flow. The most important determinants of the migration rate are the relative income differences between non-agricultural and agricultural sectors, the relative magnitude of agricultural labour, the development of terms of trade and the level of unemployment. Furthermore, the speed of economic reforms and the way of land privatization affect occupational migration significantly. An increasing intersectoral income difference points to still existing mobility restrictions for agricultural labour in some of the countries analyzed.
- Published
- 2011
9. The dynamics of the Russian lifestyle during transition: Changes in food, alcohol and cigarette consumption. ISU Economics Working Papers 09019
- Author
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Herzfeld, T., Huffman, S.K., and Rizov, M.
- Subjects
Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,WASS ,sense organs ,skin and connective tissue diseases - Abstract
This paper presents evidence on the impact of individual as well as regional characteristics on changes in fat, protein, alcohol and cigarette consumption, and on diet’s diversity between 1994 and 2004. The results from a dynamic econometric model suggest that among individual determinants such as initial levels of consumption, gender, education, household income changes, and access to a garden plot all have a significant impact on the changes in consumption behavior in Russia. Regarding the macroeconomic variables, inflation has a significant impact on changes in alcohol and cigarettes consumption, while unemployment changes significantly impact smoking behavior. Russian consumers only respond to own price changes of fat and protein, but do not respond to own prices for alcohol and cigarettes. Analysis of subsamples conditional on initial consumption behavior reveals significant heterogeneity in consumption patterns, which is important for effective policy targeting different population groups in achieving healthier lifestyle choices in Russia
- Published
- 2011
10. Labour adjustment in agriculture: Assessing the heterogeneity across transition countries
- Author
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Herzfeld, T., Dries, L.K.E., and Glauben, T.
- Subjects
Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,WASS ,Labor and Human Capital - Abstract
A standard model of labour adjustment in times of economic transition assumes a constant impact of variables like sectoral income differences, unemployment or the relative size of the agricultural sector. This paper shows for a panel of 29 European and Asian transition countries that the standard model fails to take the heterogeneity of determinants of sectoral labour adjustment properly into account. A random coefficients model reveals quite heterogeneous influences of the intersectoral income ratio, the relative size of agricultural employment, the unemployment rate, and the general level of economic development on a measure of sectoral labour adjustment across transition countries. Moreover, for selected determinants the estimated coefficients show opposing signs.
- Published
- 2011
11. Changes in Food, Alcohol and Cigarettes Consumption during Transition: Evidence from Russia
- Author
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Herzfeld, T., Huffman, S.K., Rizov, M.I., and Oskam, A.J.
- Subjects
MGS ,Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,sense organs ,skin and connective tissue diseases - Abstract
This paper examines the changes in nutritional behavior of Russian adults over the ten-year transition period, between 1994 and 2004. We present evidence on the impact of individual as well as regional characteristics on changes in fat, protein, alcohol and cigarette consumption, and on diversity of diet. The results from a dynamic empirical model suggest that among microeconomic determinants, initial levels of consumption, gender, holding a university degree, and having access to a garden plot have a significant impact on the changes in consumption behavior in Russia. Regarding the macroeconomic variables, economic growth has a significant impact on changes in fat and protein consumption and on alcohol use, while unemployment changes significantly impact protein intake, alcohol consumption and the diversity of diet.
- Published
- 2009
12. Economics of Compliance: Developing a Theoretical Framework
- Author
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Herzfeld, T. and Jongeneel, R.
- Subjects
MGS ,Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,LEI INT BELEID - Landbouwbeleid - Published
- 2008
13. Economics of compliance: a review of theories and an application to agriculture
- Author
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Herzfeld, T. and Jongeneel, R.
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MGS ,Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science ,LEI INT BELEID - Landbouwbeleid - Published
- 2008
14. Labour mobility in transition countries and the impact of institutions
- Author
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Herzfeld, T. and Glauben, T.
- Subjects
MGS ,Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science - Published
- 2006
15. Dynamics of Labour Market Participation: What Drives Chinese Farmers in and out Off-farm Employment?
- Author
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Glauben, T., Herzfeld, T., and Wang, X.
- Subjects
MGS ,Agricultural Economics and Rural Policy ,Agrarische Economie en Plattelandsbeleid ,Life Science - Published
- 2005
16. Food and Agriculture in the Slovak Republic: The Challenges of EU Accession
- Author
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Csaki, C., Nucifora, A., Lerman, Z., and Herzfeld, T.
- Subjects
voedsel ,food industry ,voedselindustrie ,slovakia ,cap ,farm structure ,tenure systems ,landbouwbeleid ,Agricultural Economics and Rural Policy ,agricultural policy ,agricultural sector ,agriculture ,gemeenschappelijk landbouwbeleid ,food ,plattelandsontwikkeling ,Agrarische Economie en Plattelandsbeleid ,slowakije ,landbouwsector ,bedrijfsstructuur in de landbouw ,landbouw ,pachtstelsel ,MGS ,agrarische economie ,agricultural economics ,rural development - Published
- 2003
17. The dynamics of food, alcohol and cigarette consumption in Russia during transition.
- Author
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Herzfeld T, Huffman S, and Rizov M
- Subjects
- Adult, Age Factors, Aged, Costs and Cost Analysis, Dietary Fats, Dietary Proteins, Feeding Behavior, Female, Gross Domestic Product, Humans, Male, Middle Aged, Residence Characteristics statistics & numerical data, Russia, Sex Factors, Socioeconomic Factors, Alcohol Drinking trends, Diet trends, Smoking trends
- Abstract
This paper presents evidence on the impact of individual as well as regional characteristics on the dynamics of fat, protein, alcohol and cigarette consumption, and on the diversity of the diet in Russia between 1994 and 2005. All those aspects of nutritional behavior are important inputs to the production of health. A dynamic panel data model is used to estimate demand functions for fat, protein, alcohol, cigarettes and diversity of the diet. The results suggest the existence of strong habits in drinking and smoking, and the absence of habits in fat and protein consumption. We also found evidence of habit formation for food diversity. Comparing nutritional behavior of younger and older consumers, we find significant differences in the demand for fat and cigarettes. Older consumers seem to be more persistent in their drinking and smoking behavior. Similarly, men show higher habit persistence for alcohol and cigarette consumption. The results also suggest that among individual determinants, especially education, income and employment have statistically significant impacts on consumption behavior. Regarding the macroeconomic variables, economic growth is negatively related to protein consumption, while regional unemployment rate is negatively affecting the demand for protein and food diversity. Finally, Russian consumers react to the price changes of alcohol, cigarettes, fat and protein as suggested by theory. Consumer demand for food diversity responds negatively to price changes of alcohol and cigarettes, but positively to the price of fat., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
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18. X-linked dystonia parkinsonism syndrome (XDP, lubag): disease-specific sequence change DSC3 in TAF1/DYT3 affects genes in vesicular transport and dopamine metabolism.
- Author
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Herzfeld T, Nolte D, Grznarova M, Hofmann A, Schultze JL, and Müller U
- Subjects
- Alternative Splicing, Calcium metabolism, Cell Line, Desmocollins metabolism, Dopamine metabolism, Dystonia genetics, Dystonia physiopathology, Exons, Genetic Diseases, X-Linked physiopathology, Histone Acetyltransferases metabolism, Humans, Introns, Neuroblastoma genetics, Neuroblastoma psychology, Parkinsonian Disorders physiopathology, TATA-Binding Protein Associated Factors metabolism, Transcription Factor TFIID metabolism, Vesicular Transport Proteins genetics, Vesicular Transport Proteins metabolism, Desmocollins genetics, Dopamine genetics, Genetic Diseases, X-Linked genetics, Histone Acetyltransferases genetics, Parkinsonian Disorders genetics, TATA-Binding Protein Associated Factors genetics, Transcription Factor TFIID genetics
- Abstract
X-chromosomal dystonia parkinsonism syndrome (XDP, 'lubag') is associated with sequence changes within the TAF1/DYT3 multiple transcript system. Although most sequence changes are intronic, one, disease-specific single-nucleotide change 3 (DSC3), is located within an exon (d4). Transcribed exon d4 occurs as part of multiple splice variants. These variants include exons d3 and d4 spliced to exons of TAF1, and an independent transcript composed of exons d2-d4. Location of DSC3 in exon d4 and utilization of this exon in multiple splice variants suggest an important role of DSC3 in the XDP pathogenesis. To test this hypothesis, we transfected neuroblastoma cells with four expression constructs, including exons d2-d4 [d2-d4/wild-type (wt) and d2-d4/DSC3] and d3-d4 (d3-d4/wt and d3-d4/DSC3). Expression profiling revealed a dramatic effect of DSC3 on overall gene expression. Three hundred and sixty-two genes differed between cells containing d2-d4/wt and d2-d4/DSC3. Annotation clustering revealed enrichment of genes related to vesicular transport, dopamine metabolism, synapse function, Ca(2+) metabolism and oxidative stress. Two hundred and eleven genes were differentially expressed in d3-d4/wt versus d3-d4/DSC3. Annotation clustering highlighted genes in signal transduction and cell-cell interaction. The data show an important role of physiologically occurring transcript d2-d4 in normal brain function. Interference with this role by DSC3 is a likely pathological mechanism in XDP. Disturbance of dopamine function and of Ca(2+) metabolism can explain abnormal movement; loss of protection against reactive oxygen species may account for the neurodegenerative changes in XDP. Although d3-d4 also affect genes potentially related to neurodegenerative processes, their physiologic role as splice variants of TAF1 awaits further exploration.
- Published
- 2013
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19. The Russian food, alcohol and tobacco consumption patterns during transition.
- Author
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Rizov M, Herzfeld T, and Huffman SK
- Subjects
- Adult, Alcohol Drinking economics, Data Collection, Dietary Fats administration & dosage, Dietary Proteins administration & dosage, Humans, Middle Aged, Models, Econometric, Russia epidemiology, Smoking economics, Socioeconomic Factors, Alcohol Drinking ethnology, Feeding Behavior ethnology, Smoking ethnology
- Abstract
The paper presents evidence on the impact of individual characteristics as well as regional macroeconomic factors on changes in fat, protein, alcohol and tobacco consumption, and on diet's diversity during the transition period 1994 - 2004 in Russia. The results from estimating first difference demand functions using Russian Longitudinal Monitoring Survey (RLMS) data suggest that individual characteristics such as initial consumption patterns, gender, education, household income, and access to a garden plot all have a significant impact on the consumption behaviour. Regarding the macroeconomic variables, inflation has a significant impact on alcohol and tobacco consumption, while unemployment significantly impacts only smoking behaviour. Russian consumers respond to own prices of fat and protein as well as to own prices of alcohol and tobacco but to a lesser extent. Analysis of subsamples based on different initial consumption patterns reveals significant heterogeneity in consumption responses.
- Published
- 2012
20. D216H polymorphism within TOR1A does not affect penetrance in DRD and is not a general modifier in primary dystonia.
- Author
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Herzfeld T, Korinthenberg R, and Müller U
- Subjects
- Dystonic Disorders genetics, Family Health, Female, Gene Frequency, Humans, Male, Aspartic Acid genetics, Histidine genetics, Molecular Chaperones genetics, Penetrance, Polymorphism, Genetic genetics
- Published
- 2011
- Full Text
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21. Maternal uniparental heterodisomy with partial isodisomy of a chromosome 2 carrying a splice acceptor site mutation (IVS9-2A>T) in ALS2 causes infantile-onset ascending spastic paralysis (IAHSP).
- Author
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Herzfeld T, Wolf N, Winter P, Hackstein H, Vater D, and Müller U
- Subjects
- Amyotrophic Lateral Sclerosis physiopathology, Base Sequence, Child, Preschool, DNA Mutational Analysis, Female, Genetic Markers, Humans, Infant, Male, Molecular Sequence Data, Pregnancy, Amyotrophic Lateral Sclerosis genetics, Chromosomes, Human, Pair 2, Guanine Nucleotide Exchange Factors genetics, Mutation, RNA Splice Sites, Uniparental Disomy
- Abstract
Infantile-onset ascending spastic paralysis (OMIM #607225) is a rare autosomal recessive early onset motor neuron disease caused by mutations in the gene ALS2. We report on a splice acceptor site mutation in intron 9 of ALS2 (IVS9-2A>T) in a German patient from nonconsanguineous parents. The mutation results in skipping of exon 10. This causes a frame-shift in exon 11 and a premature stop codon. Analysis of the parental ALS2 gene revealed heterozygosity for the mutation in the mother but not in the father. Therefore, we studied polymorphic markers scattered along chromosome 2 in both parents and the patient and found maternal uniparental disomy in the patient. While homozygosity was observed at several loci of chromosome 2 including ALS2, other loci were heterozygous, i.e., both maternal alleles were present. The findings can be explained by at least four recombination events during maternal meiosis followed by a meiosis I error and postzygotic trisomy rescue or gamete complementation.
- Published
- 2009
- Full Text
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22. Structural and functional analysis of the human TAF1/DYT3 multiple transcript system.
- Author
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Herzfeld T, Nolte D, and Müller U
- Subjects
- Alternative Splicing, Animals, Base Sequence, Brain metabolism, Cell Line, DNA Primers genetics, DNA, Complementary genetics, Evolution, Molecular, Exons, Fetus metabolism, Gene Library, Histone Acetyltransferases, Humans, Molecular Sequence Data, Primates genetics, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, TATA Box, Terminal Repeat Sequences, Transcription, Genetic, Transfection, TATA-Binding Protein Associated Factors genetics, Transcription Factor TFIID genetics
- Abstract
We analyzed TAF1/DYT3, a complex transcript system that is composed of at least 43 exons. Thirty-eight exons code for TATA box binding protein associated factor I (TAF1). Five downstream exons (d1-d5) of yet unknown function can either form transcripts with TAF1 exons or be transcribed independently. Splice variants can include d (notably d3 and d4) plus at least 12 TAF1 exons (exons 26-37 but not exon 38). These splice variants are highly polymorphic and include alternative exons (e.g., exons 30b, 31b, 32', 34', 35'). The frequency of these splice variants differs greatly in human fetal brain. Data were obtained by both RT-PCR and construction of a plasmid cDNA library. Promoter assays performed in NT2/D1 and in U87 cells demonstrate that TAF1-independent transcription of exons d2-d4 is driven by a TATA box-less promoter that is regulated by transcription factor Ikaros. Antisense transcription of exon d4 is under the control of a LTR promoter. While the 38 exons encoding TAF1 have been highly conserved in eukaryotes, the downstream exons d1-d5 were added to the transcript system much later during evolution and first appear in primates. The study demonstrates the structural and functional evolution of a complex transcript system.
- Published
- 2007
- Full Text
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23. The TAF1/DYT3 multiple transcript system in X-linked dystonia-parkinsonism.
- Author
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Muller U, Herzfeld T, and Nolte D
- Subjects
- Histone Acetyltransferases, Humans, Mutation, Polymorphism, Single Nucleotide, Dystonia genetics, Genetic Diseases, X-Linked genetics, Parkinsonian Disorders genetics, TATA-Binding Protein Associated Factors genetics, Transcription Factor TFIID genetics
- Published
- 2007
- Full Text
- View/download PDF
24. Brassicaceae contain nortropane alkaloids.
- Author
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Brock A, Herzfeld T, Paschke R, Koch M, and Dräger B
- Subjects
- Alkaloids isolation & purification, Nortropanes isolation & purification, Solanaceous Alkaloids chemistry, Solanaceous Alkaloids isolation & purification, Tropanes chemistry, Tropanes isolation & purification, Alkaloids chemistry, Brassicaceae chemistry, Brassicaceae classification, Nortropanes chemistry
- Abstract
The report of cochlearine, the 3-hydroxybenzoate ester of tropine found in Cochlearia officinalis, Brassicaceae, initiated a screening for tropane alkaloids in Cochlearia species and for calystegines in further Brassicaceae. All ten Cochlearia species investigated contained cochlearine, tropine, and pseudotropine. Calystegines, nortropane alkaloids deriving from pseudotropine, were also identified in all Cochlearia species and accumulated up to 0.5% dry mass in leaves. Brassicaceae species of all major lineages of the family were analysed for calystegines. Of the 43 species included in the study, 18 accumulated calystegines of various structures. This is the first screening of Brassicaceae for products of the tropane alkaloid pathway, which is known as characteristic for plants of Solanaceae family. The identification of calystegines in all branches of the Brassicaceae family including Aethionema, a species at the basis of the family, suggests tropane alkaloids as secondary compound typical for Brassicaceae.
- Published
- 2006
- Full Text
- View/download PDF
25. Transfer of plastid DNA from tobacco to the soil bacterium Acinetobacter sp. by natural transformation.
- Author
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de Vries J, Herzfeld T, and Wackernagel W
- Subjects
- Base Sequence, DNA genetics, Drug Resistance genetics, Genes, Plant genetics, Genome, Plant, Molecular Sequence Data, Recombination, Genetic, Soil Microbiology, Transformation, Bacterial, Acinetobacter genetics, DNA metabolism, Plastids genetics, Nicotiana genetics, Transformation, Genetic
- Abstract
Acquisition of new genetic information by horizontal gene transfer is a major mechanism of genetic adaptation and evolution in prokaryotes. Naturally transformable cells of Acinetobacter sp. were exposed to plant DNA from leaf and root tissue of transplastomic tobacco. With the aadA gene (resistance against spectinomycin and streptomycin) as anchor sequence, the transfer of segments of the tobacco plastid DNA to Acinetobacter by homology-facilitated illegitimate recombination occurred at a frequency of 1.2 x 10(-7) per cell, which was about 0.1% of the frequency of fully homologous transfers. Without anchor sequence, transfer was not detected (=1.3 x 10(-10)). The integrated plastid DNA segments extended up to 2539 nucleotides and often encompassed tobacco genes (trnL, ycf5). Expression of trnL (leucyl-tRNA) in a transformant was shown by reverse transcription polymerase chain reaction. About 44% of integration events occurred at a single hot-spot and 38% at other multiply used sites. All illegitimate recombination sites were GC-rich microhomologies of 3-6 bp often neighboured by further microhomologies. The sites were located in plant DNA at the ends of distinct larger high-GC regions, which suggests a role for GC-aided association of heterologous sequences in illegitimate DNA end joining. The results show that integration of plant DNA into a bacterial genome by natural transformation is possible and is probably stimulated by hot-spots of illegitimate recombination.
- Published
- 2004
- Full Text
- View/download PDF
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