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Sub-wavelength diameter holes in thin metal layers can exhibit remarkable optical features that make them highly suitable for (bio)sensing applications. Either as efficient light scattering centers for surface plasmon excitation or metal-clad optical waveguides, they are able to form strongly localized optical fields that can effectively interact with biomolecules and/or nanoparticles on the nanoscale. As the metal of choice, aluminum exhibits good optical and electrical properties, is easy to manufacture and process and, unlike gold and silver, its low cost makes it very promising for commercial applications. However, aluminum has been scarcely used for biosensing purposes due to corrosion and pitting issues. In this short review, we show our recent achievements on aluminum nanohole platforms for (bio)sensing. These include a method to circumvent aluminum degradation--which has been successfully applied to the demonstration of aluminum nanohole array (NHA) immunosensors based on both, glass and polycarbonate compact discs supports--the use of aluminum nanoholes operating as optical waveguides for synthesizing submicron-sized molecularly imprinted polymers by local photopolymerization, and a technique for fabricating transferable aluminum NHAs onto flexible pressure-sensitive adhesive tapes, which could facilitate the development of a wearable technology based on aluminum NHAs. [ABSTRACT FROM AUTHOR]

The development of effective array biosensors relies heavily on careful control of the density of surface-immobilized ligands on the transducing platform. In this paper we describe the synthesis of new dextran-lipase conjugates for use in immobilizing low molecular weight haptens onto glass planar waveguides for immunosensor development. The conjugates were synthesized by immobilizing bacterial thermoalkalophilic lipases (Geobacillus thermocatenulatus lipase 2, BTL2) on agarose macroporous beads, followed by covalent coupling to dextran networks of variable molecular weight (1500-40000). The chimeras were immobilized via nonspecific hydrophobic interactions onto glass planar waveguides modified with 1,1,1,3,3,3-hexamethyldisilazane to obtain highly ordered and homogeneous molecular architectures as confirmed by atomic force microscopy. Microcystin LR (MCLR) was covalently bound to the dextran-BTL2 conjugates. The usefulness of this approach in immunosensor development was demonstrated by determining amounts of MCLR down to a few picograms per liter with an automated array biosensor and evanescent wave excitation for fluorescence measurements of attached DyLight649-labeled secondary antibody. Modifying BTL2 with dextrans of an increased molecular weight (>6000) provided surfaces with an increased loading capacity that was ascribed to the production of three-dimensional surfaces by the effect of analyte binding deep in the volume, leading to expanded dynamic ranges (0.09-136.56 ng L-1), lower limits of detection (0.007 ± 0.001 ng L-1), and lower IC50 values (4.4 ± 0.7 ng L-1). These results confirm the effectiveness of our approach for the development of high-performance biosensing platforms. [ABSTRACT FROM AUTHOR]

• Novel microfluidic cartridge for an optical sensor has been designed and fabricated. • Rotary valve has been implemented for an autonomous fluid delivery system. • Detection of Irgarol 1051 and Tetracycline, as proof of concept, accomplishing EU legislation. • Optical and data acquisition integration in aMZI sensor for on-line monitoring. We have developed a photonic nano-immunosensor platform for the on-site analysis of harmful organic ocean pollutants, intended to be allocated in stand-alone buoys. The main aim is bringing the monitoring tools directly to the contaminated place, resulting in cost and time savings as compared to the standard analytical techniques. As sensor we have employed an integrated asymmetric Mach-Zehnder interferometer (aMZI) of micro/nano dimensions, based on silicon photonic technology. In order to obtain a multiplexed system, a four-channel microfluidic cell has been designed, manufactured and incorporated in the miniaturized sensor. Additionally, a microfluidic delivery module enabling automatic sample analysis has been designed, evaluated and assembled. Moreover, we have implemented the optical interconnections of the sensor chip by fiber optics, as well the electronics and the required software and data processing. Pollutant detection is based on a competitive immunoassay using bioreceptors previously biofunctionalized on the aMZI sensor arms and incubation with a specific antibody. As proof of concept, two types of pollutants have been analysed: the biocide Irgarol 1051, and the antibiotic Tetracycline. Results show limits of detection in the range of few ng/mL, accomplished the European legislation. [ABSTRACT FROM AUTHOR]

The field of environmental monitoring has experienced a substantial progress in the last years but still the on-site control of contaminants is an elusive problem. In addition, the growing number of pollutant sources is accompanied by an increasing need of having efficient early warning systems. Several years ago biosensor devices emerged as promising environmental monitoring tools, but their level of miniaturization and their fully operation outside the laboratory prevented their use on-site. In the last period, nanophotonic biosensors based on evanescent sensing have emerged as an outstanding choice for portable point-of-care diagnosis thanks to their capability, among others, of miniaturization, multiplexing, label-free detection and integration in lab-on-chip platforms. This review covers the most relevant nanophotonic biosensors which have been proposed (including interferometric waveguides, grating-couplers, microcavity resonators, photonic crystals and localized surface plasmon resonance sensors) and their recent application for environmental surveillance. [ABSTRACT FROM AUTHOR]

Cyclic olefin copolymers (COC; e.g., Zeonor, Topas, Arton, etc.) are materials with outstanding properties for developing point-of-care systems; however, the lack of functional groups in their native form makes their application challenging. This work evaluates different strategies to functionalize commercially available Zeonor substrates, including oxygen plasma treatment, photochemical grafting, and direct surface amination using an amino dextran-lipase conjugate (ADLC). The modified surfaces were characterized by contact angle measurements, Fourier transform infrared-attenuated total reflection analysis, and fluorescence assays based on evanescent wave excitation. The bioaffinity activation through the ADLC approach results in a fast, simple, and reproducible approach that can be used further to conjugate carboxylated small molecules (e.g., haptens). The usefulness of this approach has been demonstrated by the development of a heterogeneous fluorescence immunoassay to detect tacrolimus (FK506) immunosuppressant drug using an array biosensor platform based on evanescence wave laser excitation and Zeonor-ADLC substrates. Surface modification with ADLC-bearing FK506 provides a 3D layer that efficiently leads to a remarkably low limit of detection (0.02 ng/mL) and IC50 (0.9 ng/mL) together with a wide dynamic range (0.07-11.3 ng/mL).

The bimodal waveguide (BiMW) sensor is a novel common path interferometric transducer based on the evanescent field detection principle, which in combination with a bio-recognition element allows the direct detection of biomolecular interactions in a label-free scheme. Due to its inherent high sensitivity it has great potential to become a powerful analytical tool for monitoring substances of interest in areas such as environmental control, medical diagnostics and food safety, among others. The BiMW sensor is fabricated using standard silicon-based technology allowing cost-effective production, and meeting the requirements of portability and disposability necessary for implementation in a point-of-care (POC) setting.In this chapter we describe the design and fabrication of the BiMW transducer, as well as its application for bio-sensing purposes. We show as an example the biosensor capabilities two different applications: (1) the immunodetection of Irgarol 1051 biocide useful in the environmental field, and (2) the detection of human growth hormone as used in clinical diagnostics. The detection is performed in real time by monitoring changes in the intensity pattern of light exiting the BiMW transducer resulting from antigen-antibody interactions on the surface of the sensor.

We report the fabrication and performance of a surface plasmon resonance aluminum nanohole array refractometric biosensor. An aluminum surface passivation treatment based on oxygen plasma is developed in order to circumvent the undesired effects of oxidation and corrosion usually found in aluminum-based biosensors. Immersion tests in deionized water and device simulations are used to evaluate the effectiveness of the passivation process. A label-free bioassay based on biotin analysis through biotin-functionalized dextran-lipase conjugates immobilized on the biosensor-passivated surface in aqueous media is performed as a proof of concept to demonstrate the suitability of these nanostructured aluminum films for biosensing.