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Abstract Background The study analyzed microbiological and antimicrobial susceptibility profile of organisms isolated from patients with infective endocarditis (2015–17) and compared disease outcomes in cohorts of endocarditis patient with history of prior invasive vascular intervention (high risk group) vs those without (native valve group). We hypothesized that high risk group would be more likely to have severe disease outcomes. Methods This was a prospective cohort study (2015–17). All blood and cardiac tissue samples of enrolled patients suspected of endocarditis according to modified Duke’s criteria were followed for microbiological and antimicrobial susceptibility profile. The high risk group was compared with the native valve group with 90 day follow up to determine difference in clinical course and outcome in terms of disease severity (defined as any patient with endocarditis undergoing surgical management, readmission or dying). The data was analyzed using SPSS 21.0 software and chi-square test. 90 day mortality was calculated using Kaplan Meier survival curves. Results Total 104 patients with endocarditis were enrolled. Overall culture positivity rate was 71.2%. Streptococcus species were the most common isolate (36.7%), followed by S. aureus (17.3%) cases. In Streptococcus species, 14.2% showed intermediate susceptibility to penicillin. Thirty six patients were included in the cohort analysis. A poor outcome was seen in 85.7% high risk group as compared to 50% of native valve group. The overall mortality rate was 19.4%. Conclusions We found Streptococcus species to be the predominant pathogen for endocarditis overall. However Staphylococcus aureus predominated native valve group. High risk group showed more complicated clinical course.

Background This is a validation study comparing the European System for Cardiac Operative Risk Evaluation (EuroSCORE) II with the previous additive (AES) and logistic EuroSCORE (LES) and the Society of Thoracic Surgeons’ (STS) risk prediction algorithm, for patients undergoing valve replacement with or without bypass in Pakistan. Patients and Methods Clinical data of 576 patients undergoing valve replacement surgery between 2006 and 2013 were retrospectively collected and individual expected risks of death were calculated by all four risk prediction algorithms. Performance of these risk algorithms was evaluated in terms of discrimination and calibration. Results There were 28 deaths (4.8%) among 576 patients, which was lower than the predicted mortality of 5.16%, 6.96% and 4.94% by AES, LES and EuroSCORE II but was higher than 2.13% predicted by STS scoring system. For single and double valve replacement procedures, EuroSCORE II was the best predictor of mortality with highest Hosmer and Lemmeshow test (H-L) p value (0.346 to 0.689) and area under the receiver operating characteristic (ROC) curve (0.637 to 0.898). For valve plus concomitant coronary artery bypass grafting (CABG) patients actual mortality was 1.88%. STS calculator came out to be the best predictor of mortality for this subgroup with H-L p value (0.480 to 0.884) and ROC (0.657 to 0.775). Conclusions For Pakistani population EuroSCORE II is an accurate predictor for individual operative risk in patients undergoing isolated valve surgery, whereas STS performs better in the valve plus CABG group.

L'Anorexie mentale est une maladie complexe impliquant des facteurs biologiques, psychologiques et environnementaux. Le microbiote intestinal a récemment été proposé comme étant l'un de ces facteurs et pourrait jouer un rôle dans l'apparition ou la chronicité de l'Anorexie mentale. Afin de mieux comprendre le rôle potentiel du microbiote intestinal dans cette pathologie, nous avons caractérisé les altérations de la composition du microbiote dans un modèle murin d'anorexie. Des souris mâles C57Bl/6 âgées de 8 semaines ont été soumises au modèle « Activity-Based Anorexia » (ABA). Le modèle ABA reproduit de nombreuses caractéristiques de l'Anorexie mentale et a permis d'élucider plusieurs mécanismes de la physiopathologie de cette maladie. Ce modèle associe une limitation progressive du temps d'accès à la nourriture des souris (jusqu'à 3 heures/jour) et l'accès à une roue d'activité. Nous avons utilisé en contrôle des souris avec un accès libre à l'alimentation, sans roue d'activité (CTRL) et des souris avec un accès limité à l'alimentation, sans roue d'activité (LFA pour Limited Food Access) (n = 6–8 souris par groupe ; 3 expériences animales indépendantes). Nous avons caractérisé de façon détaillée la composition du microbiote intestinal des souris CTRL, LFA et ABA par séquençage haut débit des gènes ARNr 16S, complété par des analyses quantitatives en qPCR. Nous avons mis en évidence chez les souris ABA une dysbiose intestinale se caractérisant par une altération de la quantité de 76 groupes bactériens distincts (68 groupes bactériens diminués et 8 groupes bactériens augmentés chez les souris ABA par rapport aux CTRL). Nous avons en particulier pu identifier, dans le contenu caecal des souris ABA, une augmentation de la quantité de Clostridium cocleatum (× 11,9 vs CTRL, p < 0,01), une augmentation des espèces intestinale dominantes de Lactobacillus (× 5,1 vs CTRL, p < 0,001) (notamment L. reuteri , L. murinus/animalis et L. johnsonni/gasseri), ainsi qu'une diminution de la quantité de bactéries appartenant à l'ordre des Burkholderiales (× 0,4 vs CTRL, p < 0,01). De façon intéressante, nous avons pu démontrer que la grande majorité de ces modifications du microbiote (> 90 % des groupes bactériens modifiés) étaient uniquement dues à la dénutrition induite chez ces animaux et que l'activité physique n'avait pas d'effets majeurs sur la dysbiose intestinale observée. Nos travaux montrent le rôle majeur de la dénutrition dans l'établissement d'une dysbiose intestinale dans un modèle animal d'anorexie. Nos résultats constituent d'autre part une ressource de données importantes pour mieux comprendre le rôle potentiel du microbiote dans l'anorexie mentale. Ils ouvrent en particulier la voie à des manipulations du microbiote chez la souris afin de déterminer son implication dans différentes facettes de l'anorexie comme les troubles fonctionnels digestifs, la régulation de l'appétit ou certaines comorbidités comme l'anxiété et la dépression. [ABSTRACT FROM AUTHOR]

Background: Drains are used to reduce abdominal collections after procedures where such risk exists. Using abdominal drains after cholecystectomy has been controversial since the open surgery era. Universally accepted indications and agreement exist that routine drainage is unnecessary but the role of selective drainage remains undetermined. This study evaluates the indications and benefits of sub-hepatic drainage in patients undergoing laparoscopic cholecystectomy (LC) and bile duct exploration (BDE) in a specialist unit with a large biliary emergency workload., Methods: Prospectively collected data from 6,140 LCs with a 46.6% emergency workload over 30 years was reviewed. Demographic factors, pre-operative presentations, imaging and operative details in patients with and without drains were compared. Sub-hepatic drains were inserted after all transductal explorations, subtotal cholecystectomies, almost all open conversions and 94% of LC for empyemas. Adverse or beneficial postoperative drain-related outcomes were analysed., Results: Abdominal drains were utilised in 3225/6140 (52.5%). Patients were significantly older with more males. 59.4% were emergency admissions. Preoperative imaging showed thick-walled gallbladders in 25.2% and bile duct stones or dilatation in 36.2%. At operation they had cystic duct stones in 19.8%, acute cholecystitis, empyema or mucocele in 28.4% and operative difficulty grades III or higher in 59%. 38% underwent BDE, 5.4% had fundus-first dissection and the operating times were longer ( 80 vs.45 min). Drain related complications were rare; 3 abdominal pains after anaesthetic recovery settling when drains were removed, 2 drain site infections and one re-laparoscopy to retrieve a retracted drain. 55.8% of 43 bile leaks and 35% of 20 other collections in patients with drains resolved spontaneously., Conclusions: The utilisation of drains in this study was relatively high due to the high emergency workload and interest in BDE. While drains allowed early detection of bile leakage, avoiding some complications and monitoring conservative management to allow early reinterventions, the study has identified operative criteria that could potentially limit drain insertion through a selective policy., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The 'Basket-in-Catheter' (BIC) technique facilitates basket-only laparoscopic transcystic exploration (LTCE), increasing its success rate. Using the cholangiography catheter as a sheath is easier and safer than inserting the wire basket-alone. This study evaluates its benefits in confirmed and suspected ductal stones. Retrospective analysis of prospectively collected data on patients with pre-operative or operative suspicion of bile duct stones or with positive and equivocal intraoperative cholangiographies (IOC) who had LTCE attempted using blind basket trawling, without choledochoscopy, were reviewed. The incidence and outcomes of blind basket LTCEs attempted before and after introducing the BIC technique, whether or not stones were retrieved, were analysed. Blind basket LTCE was attempted in 732 patients. Of 377 (51.5%) patients undergoing successful stone retrieval, only 62% had pre-operative clinical and radiological risk factors for ductal stones, 25% had operative risk factors and 13% had silent stones discovered on IOC. Another 355 patients (48.5%) had negative trawling, although one half had pre-operative risk factors for ductal stones and 47.6% had operative risk factors, e.g. cystic duct stones or dilatation. This cohort had equivocal cholangiography in 25.9%. Following basket trawling, repeat IOC confirmed resolution of abnormalities. As no stones were retrieved, these were not considered duct explorations. The BIC technique facilitates safe and speedy bile duct clearance when stones are confirmed, avoiding choledochotomies, without significant complications. BIC duct trawling is also beneficial in patients with suspected ductal stones, helping to resolve equivocal IOCs. It helps surgeons to acquire and consolidate ductal exploration skills., (© 2023. Italian Society of Surgery (SIC).)

Precise short-term load forecasting (STLF) plays a crucial role in the smooth operation of power systems, future capacity planning, unit commitment, and demand response. However, due to its non-stationary and its dependency on multiple cyclic and non-cyclic calendric features and non-linear highly correlated metrological features, an accurate load forecasting with already existing techniques is challenging. To overcome this challenge, a novel hybrid technique based on long short-term memory (LSTM) and a modified split-convolution (SC) neural network (LSTM-SC) is proposed for single-step and multi-step STLF. The concatenating order of LSTM and SC in the proposed hybrid network provides an excellent capability of extraction of sequence-dependent features and other hierarchical spatial features. The model is evaluated by the Pakistan National Grid load dataset recorded by the National Transmission and Dispatch Company (NTDC). The load data is pre-processed and multiple other correlated features are incorporated into the data for performance enhancement. For generalization capability, the performance of LSTM-SC is evaluated on publicly available datasets of American Electric Power (AEP) and Independent System Operator New England (ISO-NE). The effect of temperature, a highly correlated input feature, on load forecasting is investigated either by removing the temperature or adding a Gaussian random noise into it. The performance evaluation in terms of RMSE, MAE, and MAPE of the proposed model on the NTDC dataset are 500.98, 372.62, and 3.72% for multi-step while 322.90, 244.22, and 2.38% for single-step load forecasting. The result shows that the proposed method has less forecasting error, strong generalization capability, and satisfactory performance on multi-horizon., Competing Interests: Khursheed Aurangzeb is an Academic Editor for PeerJ., (© 2023 Ullah et al.)

Background & Aims: Anorexia Nervosa is a severe disease depending on both biological, psychological and environmental factors. The gut microbiota has recently been proposed as one of the biological factors potentially involved in the onset or maintenance of Anorexia Nervosa. To unravel the potential role of the gut microbiota in this disease, we characterized the dysbiosis occurring in a mouse model of Anorexia and correlated bacteria level changes with different physiological parameters such as body weight, food intake or levels of hypothalamic neuropeptides., Methods: We used the Activity-Based Anorexia (ABA) mouse model, which combines food restriction and physical activity, and which mimics core features of Anorexia Nervosa. We characterized the gut microbiota alteration in ABA mice by combining 16S rRNA gene sequencing and quantitative PCR analyses of targeted genera or species., Results: We identified 68 amplicon sequence variants (ASVs) with decreased levels and 8 ASVs with increased levels in the cecal content of ABA mice compared to control mice. We observed in particular in ABA mice increases in the abundance of Clostridium cocleatum and several Lactobacillus species and a decrease in the abundance of Burkholderiales compared to control mice. Interestingly, we show that most of the observed gut microbiota alterations are due to food restriction and are not affected by physical activity. In addition, we identified several bacterial groups that correlate with mice body weight, food intake, lean and fat masses as well as with hypothalamic mRNA levels of NPY (Neuropeptide Y) and POMC (Pro-opiomelanocortin)., Conclusions: Our study provides a comprehensive characterization of the gut microbiota dysbiosis occurring in the Activity-Based Anorexia mouse model. These data constitute a valuable resource to further decipher the role of the gut microbiota in the different facets of anorexia pathophysiology, such as functional gastrointestinal disorders, appetite regulation and mood disorders., Competing Interests: Conflicts of interest PD is a co-founder of the TargEDys company; JB, PT, SH, AP, CL, JCDR, MC, LB, DR, no conflicts of interest., (Copyright © 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.)

Interleukin (IL)-22 plays a critical role in regulating the maintenance of the mucosal barrier. As airway epithelial regeneration is abnormal in cystic fibrosis (CF), we investigated IL-22 integrity in CF. We first demonstrated, using Il-22-/- mice, that IL-22 is important to prevent lung damage induced by the CF pathogen Pseudomonas aeruginosa. Next, IL-22 receptor was found normally expressed at the airway epithelial surfaces of CF patients. In wound-healing assays, IL-22-treated CF cultures had higher wound-closure rate than controls, suggesting that IL-22 signaling per se could be functional in a CF context. However, persistence of neutrophil-derived serine-proteases is a major feature of CF airways. Remarkably, IL-22 was found altered in this protease-rich inflammatory microenvironment; the serine protease-3 being the most prone to fully degrade IL-22. Consequently, we suspect an acquired deficiency of the IL-22 pathway in the lungs of CF patients due to IL-22 cleavage by the surrounding neutrophil serine-proteases., (Copyright © 2018 Elsevier Ltd. All rights reserved.)