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4. Coulomb excitation of radioactive 20, 21Na

Author

Schumaker, M. A., Cline, D., Hackman, G., Pearson, C., Svensson, C. E., Wu, C. Y., Andreyev, A., Austin, R. A. E., Ball, G. C., Bandyopadhyay, D., Becker, J. A., Boston, A. J., Boston, H. C., Buchmann, L., Churchman, R., Cifarelli, F., Cooper, R. J., Cross, D. S., Dashdorj, D., Demand, G. A., Dimmock, M. R., Drake, T. E., Finlay, P., Gallant, A. T., Garrett, P. E., Green, K. L., Grint, A. N., Grinyer, G. F., Harkness, L. J., Hayes, A. B., Kanungo, R., Lisetskiy, A. F., Leach, K. G., Lee, G., Maharaj, R., Martin, J-P., Moisan, F., Morton, A. C., Mythili, S., Nelson, L., Newman, O., Nolan, P. J., Orce, J. N., Padilla-Rodal, E., Phillips, A. A., Porter-Peden, M., Ressler, J. J., Roy, R., Ruiz, C., Sarazin, F., Scraggs, D. P., Waddington, J. C., Wan, J. M., Whitbeck, A., Williams, S. J., and Wong, J.

Published
2009
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6. Evidence of octupole-phonons at high spin in Pb

Author

Ralet, D., Clément, E., Georgiev, G., Stuchbery, A.E., Rejmund, M., Van Isacker, P., de France, G., Lemasson, A., Ljungvall, J., Michelagnoli, C., Navin, A., Balabanski, D.L., Atanasova, L., Blazhev, A., Bocchi, G., Carroll, R., Dudouet, J., Dupont, E., Fornal, B., Franchoo, S., Fransen, C., Müller-Gatermann, C., Goasduff, A., Gadea, A., John, P.R., Kocheva, D., Konstantinopoulos, T., Korichi, A., Kusoglu, A., Lenzi, S.M., Leoni, S., Lozeva, R., Maj, A., Perez, R., Pietralla, N., Shand, C., Stezowski, O., Wilmsen, D., Yordanov, D., Barrientos, D., Bednarczyk, P., Birkenbach, B., Boston, A.J., Boston, H.C., Burrows, I., Cederwall, B., Ciemala, M., Collado, J., Crespi, F., Cullen, D., Eberth, H.J., Goupil, J., Harkness, L., Hess, H., Jungclaus, A., Korten, W., Labiche, M., Menegazzo, R., Mengoni, D., Million, B., Nyberg, J., Podolyák, Zs., Pullia, A., Quintana Arnés, B., Recchia, F., Reiter, P., Saillant, F., Salsac, M.D., Sanchis, E., Theisen, C., Valiente Dobon, J.J., and Wieland, O.

Abstract

A lifetime measurement of the 19/2− state in $^{207}$Pb has been performed using the Recoil Distance Doppler-Shift (RDDS) method. The nuclei of interest were produced in multi-nucleon transfer reactions induced by a $^{208}$Pb beam impinging on a $^{100}$Mo enriched target. The beam-like nuclei were detected and identified in terms of their atomic mass number in the VAMOS++ spectrometer while the prompt γ rays were detected by the AGATA tracking array. The measured large reduced transition probability B(E3,19/2−→13/2+)=40(8) W.u. is the first indication of the octupole phonon at high spin in $^{207}$Pb. An analysis in terms of a particle-octupole-vibration coupling model indicates that the measured B(E3) value in $^{207}$Pb is compatible with the contributions from single-phonon and single particle E3 as well as E3 strength arising from the double-octupole-phonon 6+ state, all adding coherently. A crucial aspect of the coupling model, namely the strong mixing between single-hole and the phonon-hole states, is confirmed in a realistic shell-model calculation.

Published
2019

7. Evidence of octupole-phonons at high spin in Pb-207

Author

Ralet, D., Clement, E., Georgiev, G., Stuchbery, A. E., Rejmund, M., Van Isacker, R., de France, G., Lemasson, A., Ljungvall, J., Michelagnoli, C., Navin, A., Balabanski, D. L., Atanasova, L., Blazhev, A., Bocchi, G., Carroll, R., Dudouet, J., Dupont, E., Forna, B., Franchoo, S., Fransen, C., Mueller-Gatermann, C., Goasduff, A., Gadea, A., John, P. R., Kocheva, D., Konstantinopoulos, T., Korichi, A., Kusoglu, A., Lenzi, S. M., Leoni, S., Lozeva, R., Maj, A., Perez, R., Pietralla, N., Shand, C., Stezowski, O., Wilmsen, D., Yordanov, D., Barrientos, D., Bednarczyk, R., Birkenbach, B., Boston, A. J., Boston, H. C., Burrows, I, Cederwall, B., Ciemala, M., Collado, J., Crespi, F., Cullen, D., Eberth, H. J., Goupil, J., Harkness, L., Hess, H., Jungclaus, A., Korten, W., Labiche, M., Menegazzo, R., Mengoni, D., Million, B., Nyberg, Johan, Podolyak, Zs, Pullia, A., Quintana Arnes, B., Recchia, F., Reiter, R., Saillant, F., Salsac, M. D., Sanchis, E., Theisen, C., Dobon, J. J. Valiente, and Wieland, O.

Subjects
Subatomär fysik, Plunger device, VAMOS plus plus spectrometer, Subatomic Physics, Octupole phonon, AGATA spectrometer, gamma-Ray tracking, Nuclear deformation
Abstract

A lifetime measurement of the 19/2(-) state in Pb-207 has been performed using the Recoil Distance Doppler-Shift (RDDS) method. The nuclei of interest were produced in multi-nucleon transfer reactions induced by a Pb-208 beam impinging on a Mo-100 enriched target. The beam-like nuclei were detected and identified in terms of their atomic mass number in the VAMOS++ spectrometer while the prompt gamma rays were detected by the AGATA tracking array. The measured large reduced transition probability B(E3, 19/2(-) -> 13/2(+)) = 40(8) W.u. is the first indication of the octupole phonon at high spin in Pb-207. An analysis in terms of a particle-octupole-vibration coupling model indicates that the measured B(E3) value in Pb-207 is compatible with the contributions from single-phonon and single particle E3 as well as E3 strength arising from the double-octupole-phonon 6(+) state, all adding coherently. A crucial aspect of the coupling model, namely the strong mixing between single-hole and the phonon-hole states, is confirmed in a realistic shell-model calculation. Crown Copyright (C) 2019 Published by Elsevier B.V.

Published
2019

11. Induction of quiescence (GO) in bone marrow stromal stem cells enhances their stem cell characteristics

Author

RUMMAN, M, MAJUMDER, A, HARKNESS, L, VENUGOPAL, B, VINAY, MB, PILLAI, MS, KASSEM, M, and DHAWAN, J

Subjects
Suspension culture, EXPRESSION, GROWTH-FACTOR, ANCHORAGE-DEPENDENT FIBROBLASTS, Reprogramming, Cell cycle, CXC CHEMOKINE, SUBSTRATE STIFFNESS, GENE, CYCLIN-A, MYOCARDIAL-INFARCTION, BMSC, SATELLITE CELLS, Adhesion, CONTACT INHIBITION, Osteoblastic differentiation, Transcriptome, Quiescence (GO)
Abstract

Several studies have suggested that bone marrow stromal steam cells (BMSC) exist in a quiescent state (GO) within the in vivo niche; however, an explicit analysis of the biology of GO state-BMSC has not been reported. We hypothesized that induction of GO in BMSC might enhance their stem cell properties. Thus, we induced quiescence in BMSC in vitro by (a) suspension culture in a viscous medium or (b) culture on soft polyacrylamide substrate; and examined their molecular and functional phenotype. Induction of GO was confirmed by bromodeoxyuridine (BrdU) labelling and analysis of cell cycle gene expression. Upon reactivation and re-entry into cell cycle, GO state-BMSC exhibited enhanced clonogenic self-renewal, preferential differentiation into osteoblastic rather than adipocytic cells and increased ectopic bone formation when implanted subcutaneously in vivo in immune-deficient mice, compared to asynchronous proliferating (pre-GO) BMSC. Global gene expression profiling revealed reprogramming of the transcriptome during GO state including significant alterations in relevant pathways and expression of secreted factors, suggesting altered autocrine and paracrine signaling by GO state-BMSC and a possible mechanism for enhanced bone formation. GO state-BMSC might provide a clinically relevant model for understanding the in vivo biology of BMSC.

Published
2018

12. The ECM deposited by basal asthmatic and non-asthmatic ASM cells is different in composition but not biological function

Author

Harkness, L., Ashton, A., Burgess, J., Groningen Research Institute for Asthma and COPD (GRIAC), and Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)

Subjects
collagen, extracellular matrix, respiratory tract inflammation, Australia and New Zealand, Student t test, angiogenesis, cell lysate, airway remodeling, vascularization, fibronectin, tumor microenvironment, cell growth, biological functions, human, umbilical vein endothelial cell, Australian, asthma, assay, microenvironment, enzyme linked immunosorbent assay, society, cell proliferation, airway, smooth muscle fiber, cell function, protein, New Zealand
Abstract

Aim: The remodelled asthmatic airway has increased airway smooth muscle cell (ASMC) growth, expanded vasculature, and altered extracellular matrix (ECM). The ECM is the external cellular microenvironment which regulates cell behaviour. Under proliferative, inflammatory, or fibrotic conditions, the asthmaticASM cells (AASMCs) deposit altered patterns of ECM proteins. However, it is unclear whether this is the case under basal, unstimulated conditions. This study aims to examine the composition of the ECM deposited by unstimulated NA (nonasthmatic)- and AASMCs, and biological potential for modulating blood vessel formation. Methods: Primary ASMCs from asthmatic and non-asthmatic individuals were quiesced in 0.1% BSA media after 72 hr of growth. After 24 hr ASMCs were lysed and the ECM either collected in lysate buffer and quantified using BCA, or kept intact and examined for collagen and fibronectin using Picrosirius Red and ELISA, respectively. Angiogenic potential was determined by examining Human Umbilical Vein Endothelial Cell (HUVEC) proliferation on (MTT and Cyquant assays), and attachment to ASMC-derived ECM. Unpaired t-tests were utilised to identify differences between NAASMC and AASMC-derived ECM. Results: Although the total amount of ECM deposited did not change (AASM N = 4 and NAASM N = 3 respectively), AASMCs deposited more collagen (N = 5, P

Published
2015

14. Effect of cell confluence on production of cloned mice using an inbred embryonic stem cell line

Author

GAO S., MCGARRY M., FERRIER T., PALLANTE B., FLETCHER J., HARKNESS L., DE SOUSA P. A., MCWHIR J., WILMUT I., GASPARRINI, BIANCA, Gao, S., Mcgarry, M., Ferrier, T., Pallante, B., Gasparrini, Bianca, Fletcher, J., Harkness, L., DE SOUSA, P. A., Mcwhir, J., and Wilmut, I.

Subjects
developmental biology, embryo, oocyte development
Abstract

Mice have been successfully cloned from both somatic cells and hybrid embryonic stem (ES) cells. Heterozygosity of the donor ES cell genome has been suggested as a crucial factor for long-term survival of cloned mice. In the present study, an inbred ES cell line, HM-1 (129/Ola), and a well-tested ES cell line, R1 (129/Sv x 129/Sv-CP), were used as donor cells to evaluate the developmental potential of nuclear transfer embryos. We found that ES cell confluence dramatically affects the developmental potential of reconstructed embryos. With the ES cell line HM-1 and 80-90% confluence, 49% of reconstructed embryos developed to the morula/blastocyst stage, 9% of these embryos developed to live pups when transferred to the surrogate mothers, and 5 of 18 live pups survived to adulthood. By contrast, at 60-70% confluence, only 22% of embryos developed to the morula/blastocyst stage, and after transfer, only a single fetus reached term. Consistent with previous reports, the nuclei of R1 ES cells were also shown to direct development to term, but no live pups were derived from cells at later passages (>20). Our results show that the developmental potential of reconstructed embryos is determined by both cell confluence and cell passage. These results also demonstrate that the inbred ES cell line, HM-1, can be used to produce viable cloned mice, although less efficiently than most heterozygous ES cell lines.

Published
2003

15. Germinal vesicle material is essential for nucleus remodeling after nuclear transfer

Author

GAO S., MCGARRY M., FERRIER P., FLETCHER J., HARKNESS L., DE SOUSA P. A., WILMUT I., GASPARRINI, BIANCA, Gao, S., Gasparrini, Bianca, Mcgarry, M., Ferrier, P., Fletcher, J., Harkness, L., DE SOUSA, P. A., and Wilmut, I.

Abstract

Successful cloning by nuclear transfer has been reported with somatic or embryonic stem (ES) cell nucleus injection into enucleated mouse metaphase II oocytes. In this study, we enucleated mouse oocytes at the germinal vesicle (GV) or pro-metaphase I (pro-MI) stage and cultured the cytoplasm to the MII stage. Nuclei from cells of the R1 ES cell line were injected into both types of cytoplasm to evaluate developmental potential of resulting embryos compared to MII cytoplasmic injection. Immunocytochemical staining revealed that a spindle started to organize 30 min after nucleus injection into all three types of cytoplasm. A well-organized bipolar spindle resembling an MII spindle was present in both pro-MI and MII cytoplasm 1 h after injection with ES cells. However, in the mature GV cytoplasm, chromosomes were distributed throughout the cytoplasm and a much bigger spindle was formed. Pseudopronucleus formation was observed in pro-MI and MII cytoplasm after activation treatment. Although no pronucleus formation was found in GV cytoplasm, chromosomes segregated into two groups in response to activation. Only 8.1% of reconstructed embryos with pro-MI cytoplasm developed to the morula stage after culture in CZB medium. In contrast, 53.5% of embryos reconstructed with MII cytoplasm developed to the morula/blastocyst stage, and 5.3% of transferred embryos developed to term. These results indicate that GV material is essential for nucleus remodeling after nuclear transfer.

Published
2002

16. Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy

Author

DE SOUSA P. A, DOBRINSKY J. R, ZHU J., ARCHIBALD A., AINSLIE A., BOSMA W., BOWERING J., BRACKEN J., FERRIER P, FLETCHER J, HARKNESS L., GASPARRINI, BIANCA, DE SOUSA, P. A., Dobrinsky, J. R., Zhu, J., Archibald, A., Ainslie, A., Bosma, W., Bowering, J., Bracken, J., Ferrier, P, Fletcher, J, Gasparrini, Bianca, and Harkness, L.

Abstract

To clone a pig from somatic cells, we first validated an electrical activation method for use on ovulated oocytes. We then evaluated delayed versus simultaneous activation (DA vs. SA) strategies, the use of 2 nuclear donor cells, and the use of cytoskeletal inhibitors during nuclear transfer. Using enucleated ovulated oocytes as cytoplasts for fetal fibroblast nuclei and transferring cloned embryos into a recipient within 2 h of activation, a 2-h delay between electrical fusion and activation yielded blastocysts more reliably and with a higher nuclear count than did SA. Comparable rates of development using DA were obtained following culture of embryos cloned from ovulated or in vitro-matured cytoplasts and fibroblast or cumulus nuclei. Treatment of cloned embryos with cytochalasin B (CB) postfusion and for 6 h after DA had no impact on blastocyst development as compared with CB treatment postfusion only. Inclusion of a microtubule inhibitor such as nocodozole with CB before and after DA improved nuclear retention and favored the formation of single pronuclei in experiments using a membrane dye to reliably monitor fusion. However, no improvement in blastocyst development was observed. Using fetal fibroblasts as nuclear donor cells, a live cloned piglet was produced in a pregnancy that was maintained by cotransfer of parthenogenetic embryos.

Published
2002

17. Qualitative and quantitative analysis of mixtures of compounds containing both hydrogen and deuterium

Author

Crespi, H. L, Harkness, L, Katz, J. J, Norman, G, and Saur, W

Subjects
Life Sciences
Abstract

Method allows qualitative and quantitative analysis of mixtures of partially deuterated compounds. Nuclear magnetic resonance spectroscopy determines location and amount of deuterium in organic compounds but not fully deuterated compounds. Mass spectroscopy can detect fully deuterated species but not the location.

Published
1969

18. Design Considerations Of A Compton Camera For Low Energy Medical Imaging.

Author

Harkness, L. J., Boston, A. J., Boston, H. C., Cresswell, J. R., Grint, A. N., Lazarus, I., Judson, D. S., Nolan, P. J., Oxley, D. C., and Simpson, J.

Subjects
*DIAGNOSTIC imaging, *MEDICAL imaging systems, *MEDICAL imaging equipment industry, *CAMERA design & construction, *MEDICAL radiography
Abstract

Development of a Compton camera for low energy medical imaging applications is underway. The ProSPECTus project aims to utilize position sensitive detectors to generate high quality images using electronic collimation. This method has the potential to significantly increase the imaging efficiency compared with mechanically collimated SPECT systems, a highly desirable improvement on clinical systems. Design considerations encompass the geometrical optimisation and evaluation of image quality from the system which is to be built and assessed. [ABSTRACT FROM AUTHOR]

Published
2009

19. A Semiconductor-Based Positron Emission Tomography System.

Author

Oxley, D. C., Boston, A. J., Boston, H. C., Cresswell, J. R., Grint, A. N., Harkness, L. J., Jones, M., Judson, D. S., Nolan, P. J., Slee, M., Unsworth, C., and Lazarus, I. H.

Subjects
GERMANIUM, TOMOGRAPHY, GAMMA ray spectrometer, GAMMA-ray devices, COMPTON scattering, ATOMIC scattering
Abstract

This paper shall summarize the research conducted employing the high-purity germanium based small animal imaging system, SmartPET (SMall Animal Reconstructive Tomograph for Positron Emission Tomography). Geant4 simulations of the experimental setup were carried out in order to derive novel analysis procedures and quantify the system limitations. In this paper, we will focus on a gamma ray tracking approach devised to overcome germanium's high Compton scattering cross-section and on imaging challenging and complex phantom geometries. The potential of the developed tools and of the system itself will be discussed. [ABSTRACT FROM AUTHOR]

Published
2009

20. Status and Performance of an AGATA asymmetric detector.

Author

Boston, A. J., Dimmock, M. R., Unsworth, C., Boston, H. C., Cooper, R. J., Grint, A. N., Harkness, L. J., Lazarus, I. H., Jones, M., Nolan, P. J., Oxley, D. C., Simpson, J., and Slee, M.

Subjects
DETECTORS, SECURITY systems, SENSOR networks, PHYSICS instruments, GERMANIUM crystals
Abstract

High-resolution gamma-ray detectors based on high-purity germanium crystals (HPGe) are one of the key workhorses of experimental nuclear science. The technical development of such detector technology has been dramatic in recent years. Large volume, high-granularity, electrically segmented HPGe detectors have been realised and a methodology to improve position sensitivity using pulse-shape analysis coupled with the novel technique of gamma-ray tracking has been developed. Collaborations have been established in Europe (AGATA) [1] and the USA (GRETA/GRETINA) [2] to build gamma-ray tracking spectrometers. This paper discusses the performance of the first AGATA (Advanced GAmma Tracking Array) asymmetric detector that has been tested at the University of Liverpool. The use of a fully digital data acquisition system has allowed detector charge pulse shapes from a selection of well defined photon interaction positions to be analysed, yielding important information on the position sensitivity of the detector. [ABSTRACT FROM AUTHOR]

Published
2009
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23. Performance of an AGATA asymmetric detector.

Author

Boston, A. J., Dimmock, M. R., Unsworth, C., Boston, H. C., Cooper, R. J., Grint, A. N., Harkness, L. J., Lazarus, I. H., Jones, M., Nolan, P. J., Oxley, D. C., Simpson, J., and Slee, M.

Subjects
GAMMA rays, SPECTRUM analysis, GERMANIUM crystals, PHOTONS, ELECTROMAGNETIC waves
Abstract

Each major technical advance in gamma-ray detection devices has resulted in significant new insights into the structure of atomic nuclei. The next major step in gamma-ray spectroscopy involves achieving the goal of a 4π ball of germanium detectors by using the technique of gamma-ray energy tracking in electrically segmented germanium crystals. The resulting spectrometer will have an unparalleled level of detection power for nuclear electromagnetic radiation. Collaborations have been established in Europe (AGATA) [1] and the USA (GRETA/GRETINA) [2] to build gamma-ray tracking spectrometers. This paper discusses the performance of the first AGATA (Advanced GAmma Tracking Array) asymmetric detector that has been tested at the University of Liverpool. The use of a fully digital data acquisition system has allowed detector charge pulse shapes from a selection of well defined photon interaction positions to be analysed, yielding important information on the position sensitivity of the detector. [ABSTRACT FROM AUTHOR]

Published
2008
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25. Soy and bone: where do we stand?

Author

Harkness L

Abstract

Interest in the use of plant estrogens (phytoestrogens) to attenuate postmenopausal bone loss has increased during the past 10 years as healthcare consumers, practitioners, and scientists have searched for alternative treatments to the use of hormone replacement therapy. Promising research has emerged regarding the potential benefit of increased consumption of phytoestrogens, primarily from dietary soy products. Dietary phytoestrogens, primarily isoflavones found in soy foods, may alter bone turnover in postmenopausal women by decreasing bone resorption and increasing bone formation. [ABSTRACT FROM AUTHOR]

Published
2004
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28. Morphological and molecular characteristics of living human fetuses between Carnegie stages 7 and 23: localization of inhibin mRNA alpha and beta a subunits by in-situ hybridization.

Author

Harkness, LM, Baird, DT, Harkness, L M, and Baird, D T

Subjects
EMBRYO anatomy, RNA analysis, BONES, CARDIOVASCULAR system, DIGESTIVE organs, DNA probes, GENITOURINARY organs, GESTATIONAL age, GLYCOPROTEINS, HUMAN reproduction, IN situ hybridization, NERVOUS system, SKIN, EMBRYOS
Abstract

Localization of the mRNA α and βa subunits of inhibin has previously been reported in the human gonads during the second trimester. Adrenal inhibin has also been reported in the second trimester for the α βa and βb subunits. Investigations showing localization by in-situ hybridization during the first trimester have not been reported. The results have shown hybridization of the α and βa subunits, throughout the period of development studied, in a variety of tissues including the dorsal and thoracic aortas and pericardium stages 13-22 (βa subunit); liver stages 19-21 (βa) and stages 21-22 (α); mesonephros stages 21 and 22 (βa); gonad stages (α and βa); adrenal stages 19-22 (α); surface ectoderm stages 16-22 (βa); mesenchyme stages 16-22 (βa); amnion stages 13-16 (βa); yolk sac stage 12 (α and βa); cartilage stages 19-22 (βa); and nasal proliferation stages 21 and 22 (βa). When compared with distribution of the protein subunits it was noted that more immunostaining activity was found, suggesting that probes were not sufficiently sensitive enough to detect all levels of mRNA expressed. It can be surmised, therefore, that the lack of visual hybridization of the mRNA cannot preclude the possibility that it is not being translated within the tissue even though hybridization was not apparent. [ABSTRACT FROM PUBLISHER]

Published
1997
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29. Morphological and molecular characteristics of living human fetuses between Carnegie stages 7 and 23: immunolocalization of inhibin alpha and beta a subunits.

Author

Harkness, LM, Baird, DT, Harkness, L M, and Baird, D T

Subjects
GLYCOPROTEIN analysis, ADRENAL glands, BONES, CARDIOVASCULAR system, DIGESTIVE organs, GENITOURINARY organs, GESTATIONAL age, IMMUNOHISTOCHEMISTRY, NERVOUS system, PEPTIDE hormones, RESPIRATORY organs, SKIN, EMBRYOS
Abstract

Transforming growth factor (TGF) is known to have the ability to modify mitogenic responses of tissues to other peptide growth factors and therefore may contribute to the rapid growth rate of an embryo. Throughout the TGF superfamily there is a similar fundamental molecular architecture. Included in this superfamily are inhibin A, activin A and activin B. It has been shown that activin is a powerful mesodermal inducing factor in the early embryo. The human embryo has shown localization of inhibin in the gonads after 16 weeks gestation but it has not been previously identified in earlier embryos. The inhibin-activin protein was found in a range of tissues including the liver stages 19-21 (α) and stages 19-22 (β); oesophagus stages 19-22 (α and β); stomach stages 21 and 22 (α and β); gut stages 16-22 (α) and 21 and 22 (β); pericardium stages 12-22 (α and β); gonad stages 21 and 22 (β) stage 22 (α); adrenal stages 19-22 (α and β); urogenital system states 21 and 22 (α and β); yolk sac stage 12 (α and β); mesenchyme stages 16-22 (α); surface ectoderm stages 13-22 (α) and stages 16-22 (βa); notochord stages 13-22 (β) and stages 21 and 22 (α); nasal, trachea and bronchi stages 19-22 (α and β) leading to speculation of the role of both subunits. [ABSTRACT FROM PUBLISHER]

Published
1997
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30. Morphological and molecular characteristics of living human fetuses between Carnegie stages 7 and 23: ultrasound scanning and direct measurements.

Author

Harkness, LM, Rodger, M, Baird, DT, Harkness, L M, and Baird, D T

Abstract

The developmental age of an embryo in the first trimester of pregnancy is generally determined by ultrasound scanning and/or by calculation from menstrual age. In the original studies, validation of the estimate of gestational age by ultrasound was not possible as the exact date of conception was unknown. Variation in growth rates of identically aged fetuses has previously been reported after assisted conception and with the use of ultrasound scanning. As these pregnancies were ongoing the accuracy of the scanning results could not be determined. Comparison of scanning and direct measurements after termination of pregnancy and menstrual age were carried out to determine the accuracy in fetal dating. The results suggest that the use of ultrasound scanning to determine gestational age is of less use than previously thought, and that the use of menstrual age is severely limited. [ABSTRACT FROM AUTHOR]

Published
1997
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32. Cytogenetic analysis of unfertilized human oocytes.

Author

Angell, R.R., Ledger, W., Yong, E.L., Harkness, L., and Baird, D.T.

Abstract

Cytogenetic studies were carried out on 180 oocytes that appeared unfertilized after in-vitro fertilization. The majority of the 135 that were informative had grossly haploid second meiotic metaphases, two were grossly diploid, and five had a variety of different abnormalities. Twenty-one oocytes were abnormally fertilized and included prematurely condensed sperm chromosomes. The frequency of this phenomenon varied according to the stimulation protocol, those oocytes maturing longer in vivo showing less propensity to abnormal fertilizations. Thirteen per cent of the analysable haploid metaphases were hyperhaploid but none contained extra whole chromosomes. The extra components were a single chromatid (one case), or two single chromatids replacing a whole chromosome (four cases). The data suggest that the chromatids arose as a result of premature centromere division at meiosis I, and that this may be a major mechanism for trisomy formation rather than non-disjunction of whole bivalents at meiosis I, as generally believed. [ABSTRACT FROM AUTHOR]

Published
1991

38. A CARD.

Author

HARKNESS, L. G.

Published
1857

39. AGATA-Advanced GAmma Tracking Array

Author

A. Colombo, Y. Mariette, A. Johnson, W. Korten, A. Brondi, T. Faul, J. Robin, R. Depalo, M. Slee, J. Gre¸bosz, H. C. Boston, H. Harroch, B. Rossé, M. Filliger, M. Gulmini, A. Korichi, S. Brambilla, J.R. Cresswell, C. Parisel, P. Edelbruck, F. Ameil, F. Camera, C. Oziol, Matthew Richard Dimmock, G. Salvato, F. Morbiducci, J. Ropert, M. Borsato, E. Legay, Marco Bellato, C. Santos, Gheorghe Pascovici, L. Charles, J. Pancin, G. Casati, P. G. Bizzeti, T. Stanios, S. Lhenoret, Diego Barrientos, P.M. Jones, Andreas Görgen, V. Chambert, S. Aydin, K. Hauschild, B. Hervieu, R. Nicolini, J. Simpson, H. J. Wollersheim, L. Lavergne, Pär-Anders Söderström, G. S. Simpson, G. Jaworski, F. Filmer, R. Griffiths, P.S. Morrall, M. Petcu, G. Lo Bianco, D. Linget, A. Givechev, D. C. Oxley, M. Kogimtzis, F. Salomon, J. Jolie, D. Wells, S. Moon, C. Aufranc, A. Corsi, T. Descombes, N. Goel, J. Thornhill, N. Warr, Serkan Akkoyun, B. Birkenbach, F. Dorangeville, Joël Chavas, P. Medina, Roberto Isocrate, C. Weber, E. Merchan, Bo Cederwall, I. Kojouharov, Enrique Sanchis, A. Lopez-Martens, Oliver Wieland, F. Le Blanc, D. Seddon, S. C. Letts, D. Bazzacco, J. Leske, B. Bruyneel, Alberto Pullia, L. Nelson, G. La Rana, B. Travers, I. Burrows, G. Maron, S. Coelli, J. J. Valiente-Dobón, F. Saillant, Piotr Bednarczyk, V.L. Ngo, J. Mierzejewski, Norbert Pietralla, R.S. Kempley, N. Kurz, M. Rebeschini, S. Fantinel, D. L. Balabanski, A. J. Boston, F. Tomasi, R. Berthier, M. D. Salsac, B. Dulny, C. Stahl, N. Dosme, C. Fanin, R. Baumann, S. Pietri, A. Astier, M. Nicoletto, J. van der Marel, A. Bracco, Björn Jonson, D. Mengoni, D. Bloor, Luna Pellegri, F. Lefebvre, D. Pugnére, J. Egea, S. Cabaret, D. Curien, M. Reese, C. Commeaux, Dirk Rudolph, A. Giannatiempo, F. Recchia, L. Ramina, A. Lermitage, C. A. Ur, R. M. Lieder, I.P. Brawn, R. Menegazzo, R. Raine, Krasimir Mitev, D. R. Napoli, G. Suliman, M. Kebbiri, Y. Drouen, Susan Rigby, W. Me¸czyński, R. Wadsworth, F. Didierjean, P. Desesquelles, Ch. Veyssiere, S. Leoni, Vicente González, P. Gros, Y. Le Noa, M. Castoldi, O. Möller, R. Orlandi, Thomas Beck, M. Şenyiğit, A. Jungclaus, L. Gibelin, Mohammed Kaci, Zs. Podolyák, J. Gerl, S. Erturk, W. Gast, B. Melon, V. Mendéz, R. Peghin, P. Molini, M. N. Erduran, D. Conventi, Pankaj S. Joshi, E. Clément, F.A. Beck, A. Austin, A. Cortesi, J. Palin, P. Cocconi, L. J. Harkness, F. C. L. Crespi, Dirk Weisshaar, P. Sona, A. Czermak, D. Lersch, N. Blasi, Anton Khaplanov, O. Stezowski, B. Rubio, R. Venturelli, M.-H. Sigward, E. Viscione, José Salt, C. S. Özben, Giancarlo Ripamonti, P. Spolaore, Johan Nyberg, S. M. Lenzi, L. Legeard, N. Redon, C. Rossi Alvarez, C. He, R. Chapman, P. Le Pouhalec, C. Diarra, Herbert Hess, T. Engert, A. Kaşkaş, Stanislav Tashenov, S. Klupp, C. Michelagnoli, Elif Ince, A. Algora, J. F. Smith, M. Ozille, Alessandro Zucchiatti, Angelo Geraci, A. Olariu, M. Labiche, N. Karkour, Stefano Riboldi, P. Bourgault, C. M. Petrache, M. Palacz, M. El Chambit, J.-L. Cercus, M. Zie¸bliński, Alex Wiens, R. Beunard, X. Lafay, T. Habermann, M. Karolak, L. Costa, Y. Aubert, Begoña Quintana, A P Robinson, V. F. E. Pucknell, D. Rosso, A. Capsoni, Emanuele Vardaci, L. Mihailescu, M. Tripon, E. Farnea, Ch. Theisen, Enrico Calore, G. Duchêne, N. V. Zamfir, X. Grave, S. Lunardi, I.H. Lazarus, José Blasco, D. Montanari, J.A. Sampson, P. H. Regan, N. Marginean, M. Pignanelli, L. Arnold, G. Benzoni, R. Krücken, G. de Angelis, D.P. Scraggs, I. Piqueras, D. M. Cullen, A. Obertelli, A. Bürger, D. Bortolato, G. Rainovski, A.R. Mather, P. Reiter, C. Unsworth, G. Baulieu, P. J. Coleman-Smith, C. Boiano, M. Richer, Sébastien Perrier, Agnese Giaz, Reynold J. Cooper, V. Vandone, S. Tanguy, C. Domingo-Pardo, A. Bouty, A. N. Grint, A. Maj, A. Gottardo, L. Berti, E. Pachoud, R. Marginean, E. A. Stefanova, A. Triossi, M. Nespolo, D. Bucurescu, R. Moro, H.T.M. Ha, D. S. Judson, J. L. Tain, A. Perego, Francesco Lelli, P. Detistov, B.Y. Ky, J. Ljungvall, B. Sowicki, M. Schlarb, F. Azaiez, P. Pariset, Ertan Şahin, R. Gernhäuser, M. Turcato, John Paul Strachan, B. Million, A. Atac, F. Carrio, D. Delbourg, F. Veronese, Th. Kröll, B. Cahan, A. Hernandez-Prieto, M. A. Bentley, K.M.M. Tun-Lanoë, B. Alikhani, P. Boutachkov, S. Leboutelier, Maria Doncel, A. M. Bizzeti-Sona, R. Touzery, A. Nannini, A. Lotode, P. J. Nolan, G. Rampazzo, F. Zocca, H. Schaffner, J. Eberth, A. Gadea, N. Toniolo, T. Hüyük, Q.T. Doan, P. Petkov, S. Broussard, S. Badoer, M. Rigato, Philip M Walker, J. Pouthas, Ch. Finck, L. Milechina, M. Norman, E. Pierre, J. Roccaz, S.J. Colosimo, Lázaro Guevara, Glyn Wittwer, Millan, Vicente Gonzalez -- 0000-0001-6014-2586, Rainovski, Georgi -- 0000-0002-1729-0249, Jaworski, Grzegorz -- 0000-0003-2241-0329, KORTEN, Wolfram -- 0000-0002-3940-0816, Domingo-Pardo, Cesar -- 0000-0002-2915-5466, Triossi, Andrea -- 0000-0001-5140-9154, Huyuk, Tayfun -- 0000-0003-0597-9767, Ince, Elif -- 0000-0003-4821-5441, Algora, Alejandro -- 0000-0002-5199-1794, Calore, Enrico -- 0000-0002-2301-3838, Rubio, Berta -- 0000-0002-9149-4151, THEISEN, Christophe -- 0000-0002-8509-1022, Rudolph, Dirk -- 0000-0003-1199-3055, Gadea, Andres -- 0000-0002-4233-1970, santos, cayetano -- 0000-0003-0727-1914, nannini, adriana -- 0000-0003-0659-7648, Depalo, Rosanna -- 0000-0003-3943-7982, Suliman, gabriel -- 0000-0001-8475-1992, Blasco, Jose-Maria -- 0000-0002-7663-9092, Petrache, Costel -- 0000-0001-8419-1390, Pietralla, Norbert -- 0000-0002-4797-3032, Cederwall, Bo -- 0000-0003-1771-2656, Zucchiatti, Alessandro -- 0000-0002-3647-596X, Mendez Munoz, Victor -- 0000-0002-9044-1189, Kruecken, Reiner -- 0000-0002-2755-8042, Napoli, Daniel Ricardo -- 0000-0002-8154-6958, Tain, Jose L. -- 0000-0002-3263-6965, SANCHIS, ENRIQUE -- 0000-0002-9689-9131, Zieblinski, Miroslaw -- 0000-0002-8693-7317, Hauschild, Karl -- 0000-0003-2862-2445, Montanari, Daniele -- 0000-0002-1980-7686, senyigit, menekse -- 0000-0002-2408-4419, La Rana, Giovanni -- 0000-0003-2814-4113, Menegazzo, Roberto -- 0000-0002-3060-5276, Soderstrom, Par-Anders -- 0000-0002-9504-2814, Unsworth, Carl -- 0000-0002-4100-7466, Camera, Franco -- 0000-0003-1731-4834, Gorgen, Andreas -- 0000-0003-1916-9941, Hernandez Prieto, Alvaro -- 0000-0002-0340-0240, Recchia, Francesco -- 0000-0002-8428-0112, de France, Gilles -- 0000-0002-7439-1759, GOTTARDO, Andrea -- 0000-0002-0390-5767, Perrier, Sebastien -- 0000-0001-5055-9046, Sowicki, Bogdan -- 0000-0002-7208-0690, Robinson, Andrew -- 0000-0002-2510-1321, Sahin, Eda -- 0000-0003-0683-5140, Pullia, Alberto -- 0000-0002-6393-747X, Lelli, Francesco -- 0000-0003-1900-9171, Clement, Emmanuel -- 0000-0003-1887-717X, benzoni, giovanna -- 0000-0002-7938-0338, Jonson, Bjorn -- 0000-0002-9697-9115, Bednarczyk, Piotr -- 0000-0002-5699-5292, Jones, Pete -- 0000-0001-7480-6603, Nicoletto, Marino -- 0000-0001-9301-0782, Ripamonti, Giancarlo -- 0000-0002-9406-021X, Bentley, Michael -- 0000-0001-8401-3455, Pellegri, Luna -- 0000-0002-3227-3332, Barrientos, Diego -- 0000-0001-9693-2942, GIAZ, Agnese -- 0000-0002-2550-450X, [Boston, A. J. -- Boston, H. C. -- Colosimo, S. -- Cooper, R. J. -- Cresswell, J. R. -- Dimmock, M. R. -- Filmer, F. -- Grint, A. N. -- Harkness, L. J. -- Judson, D. S. -- Mather, A. R. -- Moon, S. -- Nelson, L. -- Nolan, P. J. -- Norman, M. -- Oxley, D. C. -- Rigby, S. -- Sampson, J. -- Scraggs, D. P. -- Seddon, D. -- Slee, M. -- Stanios, T. -- Thornhill, J. -- Unsworth, C. -- Wells, D.] Univ Liverpool, Oliver Lodge Lab, Liverpool L69 7ZE, Merseyside, England -- [Akkoyun, S. -- Atac, A. -- Kaskas, A. -- Senyigit, M.] Ankara Univ, Fac Sci, Dept Phys, TR-06100 Ankara, Turkey -- [Algora, A. -- Barrientos, D. -- Domingo-Pardo, C. -- Egea, J. -- Gadea, A. -- Hueyuek, T. -- Kaci, M. -- Mendez, V. -- Rubio, B. -- Salt, J. -- Tain, J. L.] Univ Valencia, CSIC, IFIC, E-46980 Paterna, Spain -- [Alikhani, B. -- Boutachkov, P. -- Givechev, A. -- Goel, N. -- Leske, J. -- Merchan, E. -- Moeller, O. -- Pietralla, N. -- Reese, M. -- Stahl, C.] Tech Univ Darmstadt, IKP, D-64289 Darmstadt, Germany -- [Ameil, F. -- Beck, T. -- Boutachkov, P. -- Domingo-Pardo, C. -- Engert, T. -- Gerl, J. -- Goel, N. -- Habermann, T. -- Kojouharov, I. -- Kurz, N. -- Merchan, E. -- Pietri, S. -- Schaffner, H. -- Tashenov, S. -- Wollersheim, H. J.] GSI Helmholtzzentrum Schwerionenforsch GmbH, D-64291 Darmstadt, Germany -- [de Angelis, G. -- Badoer, S. -- Berti, L. -- Calore, E. -- Cocconi, P. -- Conventi, D. -- Costa, L. -- Fantinel, S. -- Gadea, A. -- Gottardo, A. -- Gulmini, M. -- He, C. -- Ince, E. -- Kroell, Th. -- Lelli, F. -- Maron, G. -- Molini, P. -- Napoli, D. R. -- Rigato, M. -- Rosso, D. -- Sahin, E. -- Spolaore, P. -- Toniolo, N. -- Valiente-Dobon, J. J.] Ist Nazl Fis Nucl, Lab Nazl Legnaro, IT-35020 Padua, Italy -- [Arnold, L. -- Baumann, R. -- Beck, F. A. -- El Chambit, M. -- Charles, L. -- Curien, D. -- Didierjean, F. -- Duchene, G. -- Faul, T. -- Filliger, M. -- Finck, Ch. -- Medina, P. -- Pachoud, E. -- Parisel, C. -- Piqueras, I. -- Richer, M. -- Robin, J. -- Santos, C. -- Sigward, M. -H. -- Weber, C.] Univ Strasbourg, IPHC, F-67037 Strasbourg, France -- [Arnold, L. -- Baumann, R. -- Beck, F. A. -- El Chambit, M. -- Charles, L. -- Curien, D. -- Didierjean, F. -- Duchene, G. -- Faul, T. -- Filliger, M. -- Finck, Ch. -- Medina, P. -- Pachoud, E. -- Parisel, C. -- Piqueras, I. -- Richer, M. -- Robin, J. -- Santos, C. -- Sigward, M. -H. -- Weber, C.] CNRS, UMR 7178, F-67037 Strasbourg, France -- [Astier, A. -- Cabaret, S. -- Desesquelles, P. -- Dosme, N. -- Gibelin, L. -- Ha, H. T. M. -- Hauschild, K. -- Karkour, N. -- Korichi, A. -- Lafay, X. -- Leboutelier, S. -- Legay, E. -- Lhenoret, S. -- Linget, D. -- Ljungvall, J. -- Lopez-Martens, A. -- Morbiducci, F. -- Ngo, V. L. -- Pariset, P. -- Perrier, S. -- Pierre, E. -- Roccaz, J. -- Travers, B.] Univ Paris 11, CNRS, IN2P3, CSNSM, F-91405 Orsay, France -- [Atac, A. -- Nyberg, J. -- Soderstrom, P. -A.] Uppsala Univ, Dept Phys & Astron, Uppsala, Sweden -- [Atac, A. -- Cederwall, B. -- Johnson, A. -- Khaplanov, A. -- van der Marel, J. -- Milechina, L. -- Tashenov, S.] Royal Inst Technol, SE-10691 Stockholm, Sweden -- [Aubert, Y. -- Azaiez, F. -- Le Blanc, F. -- Cercus, J. -L. -- Chambert, V. -- Commeaux, C. -- Delbourg, D. -- Diarra, C. -- Dorangeville, F. -- Edelbruck, P. -- Grave, X. -- Guevara, L. -- Harroch, H. -- Ky, B. Y. -- Lavergne, L. -- Lefebvre, F. -- Lermitage, A. -- Olariu, A. -- Oziol, C. -- Petrache, C. -- Pouthas, J. -- Salomon, F. -- Tanguy, S. -- Tun-Lanoe, K. M. M.] Univ Paris 11, CNRS, IN2P3, IPNO, F-91406 Orsay, France -- [Aufranc, C. -- Baulieu, G. -- Doan, Q. T. -- Pugnere, D. -- Redon, N. -- Rosse, B. -- Stezowski, O.] Univ Lyon 1, CNRS, IN2P3, Inst Phys Nucl Lyon, F-69622 Villeurbanne, France -- [Austin, A. -- Burrows, I. -- Coleman-Smith, P. J. -- Griffiths, R. -- Kogimtzis, M. -- Labiche, M. -- Lazarus, I. H. -- Letts, S. C. -- Morrall, P. S. -- Palin, J. -- Pucknell, V. F. E. -- Simpson, J. -- Strachan, J.] STFC Daresbury Lab, Warrington WA4 4AD, Cheshire, England -- [Aydin, S. -- Bazzacco, D. -- Bellato, M. -- Bortolato, D. -- Chavas, J. -- Colombo, A. -- Fanin, C. -- Farnea, E. -- Isocrate, R. -- Kroell, Th. -- Lenzi, S. M. -- Lunardi, S. -- Marginean, R. -- Menegazzo, R. -- Mengoni, D. -- Michelagnoli, C. -- Nespolo, M. -- Nicoletto, M. -- Peghin, R. -- Ramina, L. -- Rampazzo, G. -- Rebeschini, M. -- Recchia, F. -- Rossi Alvarez, C. -- Salvato, G. -- Triossi, A. -- Turcato, M. -- Ur, C. A. -- Venturelli, R. -- Veronese, F.] Ist Nazl Fis Nucl, Sez Padova, IT-35131 Padua, Italy -- [Balabanski, D. L. -- Detistov, P. -- Petkov, P. -- Stefanova, E.] Bulgarian Acad Sci, Inst Nucl Res & Nucl Energy, Sofia, Bulgaria -- [Bednarczyk, P. -- Czermak, A. -- Dulny, B. -- Grebosz, J. -- Maj, A. -- Meczynski, W. -- Sowicki, B. -- Zieblinski, M.] Polish Acad Sci, Henryk Niewodniczanski Inst Nucl Phys, PL-31342 Krakow, Poland -- [Bentley, M. A. -- Bloor, D. -- Joshi, P. -- Wadsworth, R.] Univ York, Dept Phys, York YO10 5DD, N Yorkshire, England -- [Benzoni, G. -- Blasi, N. -- Boiano, C. -- Bracco, A. -- Brambilla, S. -- Camera, F. -- Capsoni, A. -- Casati, G. -- Coelli, S. -- Corsi, A. -- Cortesi, A. -- Crespi, F. C. L. -- Geraci, A. -- Giaz, A. -- Leoni, S. -- Million, B. -- Montanari, D. -- Nicolini, R. -- Pellegri, L. -- Pignanelli, M. -- Pullia, A. -- Riboldi, S. -- Ripamonti, G. -- Tomasi, F. -- Vandone, V. -- Viscione, E. -- Wieland, O. -- Zocca, F.] Ist Nazl Fis Nucl, Sez Milano, IT-20133 Milan, Italy -- [Berthier, R. -- Bouty, A. -- Broussard, S. -- Buerger, A. -- Drouen, Y. -- Gros, P. -- Goergen, A. -- Hervieu, B. -- Karolak, M. -- Kebbiri, M. -- Korten, W. -- Ljungvall, J. -- Lotode, A. -- Mariette, Y. -- Le Noa, Y. -- Obertelli, A. -- Le Pouhalec, P. -- Salsac, M. -D. -- Theisen, Ch. -- Touzery, R. -- Veyssiere, Ch.] CEA, Ctr Saclay, IRFU, F-91191 Gif Sur Yvette, France -- [Beunard, R. -- Bourgault, P. -- Cahan, B. -- Clement, E. -- de France, G. -- Legeard, L. -- Ozille, M. -- Pancin, J. -- Raine, R. -- Ropert, J. -A. -- Saillant, F. -- Tripon, M. -- Wittwer, G.] CEA DSM CNRS IN2P3, GANIL, F-14076 Caen, France -- [Birkenbach, B. -- Bruyneel, B. -- Eberth, J. -- Hess, H. -- Jolie, J. -- Lersch, D. -- Pascovici, G. -- Reiter, P. -- Warr, N. -- Weisshaar, D. -- Wiens, A.] Univ Cologne, IKP, D-50937 Cologne, Germany -- [Bizzeti, P. G. -- Bizzeti-Sona, A. M. -- Giannatiempo, A. -- Melon, B. -- Perego, A. -- Sona, P.] Univ Florence, Dipartimento Fis & Astron, IT-50019 Florence, Italy -- [Bizzeti, P. G. -- Bizzeti-Sona, A. M. -- Giannatiempo, A. -- Melon, B. -- Nannini, A. -- Perego, A. -- Sona, P.] Ist Nazl Fis Nucl, Sez Firenze, IT-50019 Florence, Italy -- [Blasco, J. M. -- Carrio, F. -- Egea, J. -- Gonzalez, V. -- Sanchis, E.] Univ Valencia, Dept Elect Engn, E-46100 Burjassot, Valencia, Spain -- [Borsato, M. -- Bortolato, D. -- Lenzi, S. M. -- Lunardi, S. -- Marginean, R. -- Mengoni, D. -- Michelagnoli, C. -- Nespolo, M. -- Recchia, F. -- Salvato, G. -- Triossi, A. -- Venturelli, R.] Univ Padua, Dipartimento Fis, IT-35131 Padua, Italy -- [Bracco, A. -- Camera, F. -- Corsi, A. -- Crespi, F. C. L. -- Giaz, A. -- Leoni, S. -- Montanari, D. -- Nicolini, R. -- Pellegri, L. -- Pignanelli, M. -- Pullia, A. -- Riboldi, S. -- Vandone, V.] Univ Milan, Dipartimento Fis, IT-20133 Milan, Italy -- [Brawn, I. P.] STFC Rutherford Appleton Lab, Didcot OX11 0QX, Oxon, England -- [Brondi, A. -- Moro, R. -- La Rana, G. -- Vardaci, E.] Univ Naples Federico II, Dipartimento Fis, IT-80126 Naples, Italy -- [Brondi, A. -- Moro, R. -- La Rana, G. -- Vardaci, E.] Ist Nazl Fis Nucl, Sez Napoli, IT-80126 Naples, Italy -- [Bucurescu, D. -- Marginean, N. -- Marginean, R. -- Petcu, M. -- Ur, C. A.] Natl Inst Phys & Nucl Engn, Bucharest, Romania -- [Buerger, A. -- Suliman, G. -- Zamfir, N. V.] Univ Bonn, Helmholtz Inst Strahlen & Kernphys, D-53115 Bonn, Germany -- [Buerger, A.] Univ Oslo, Dept Phys, N-0316 Oslo, Norway -- [Casati, G. -- Geraci, A. -- Ripamonti, G.] Politecn Milan, Dipartimento Elettron & Informaz, IT-20133 Milan, Italy -- [Castoldi, M. -- Zucchiatti, A.] Ist Nazl Fis Nucl, Sez Genova, IT-16146 Genoa, Italy -- [Chapman, R. -- Mengoni, D. -- Orlandi, R. -- Smith, J. F.] Univ W Scotland, Sch Engn, Paisley PA1 2BE, Renfrew, Scotland -- [Cullen, D. M. -- Robinson, A. P.] Univ Manchester, Sch Phys & Astron, Schuster Lab, Manchester M13 9PL, Lancs, England -- [Depalo, R.] Univ Padua, Dipartimento Astron, IT-35131 Padua, Italy -- [Descombes, T. -- Simpson, G.] Univ Grenoble 1, CNRS, IN2P3, LPSC,INP Grenoble, F-38026 Grenoble, France -- [Doncel, M. -- Hernandez-Prieto, A. -- Quintana, B.] Univ Salamanca, Dept Fis Fundamental, E-37008 Salamanca, Spain -- [Erduran, M. N. -- Ince, E.] Istanbul Univ, Istanbul, Turkey -- [Erturk, S.] Nigde Univ, Fac Sci, Dept Phys, TR-51200 Nigde, Turkey -- [Gast, W. -- Lieder, R. M. -- Mihailescu, L.] Forschungszentrum Julich, Inst Kernphys, D-52425 Julich, Germany -- [Gernhaeuser, R. -- Klupp, S. -- Kruecken, R. -- Schlarb, M.] Tech Univ Munich, Phys Dept E12, D-85748 Garching, Germany -- [Jaworski, G.] Warsaw Univ Technol, Fac Phys, PL-00662 Warsaw, Poland -- [Jaworski, G. -- Mierzejewski, J. -- Palacz, M.] Univ Warsaw, Heavy Ion Lab, PL-02093 Warsaw, Poland -- [Jones, P.] Univ Jyvaskyla, Dept Phys, FI-40014 Jyvaskyla, Finland -- [Jonson, B.] Chalmers, S-41296 Gothenburg, Sweden -- [Jungclaus, A. -- Orlandi, R.] CSIC, Inst Estruct Mat, E-28006 Madrid, Spain -- [Kempley, R. S. -- Podolyak, Z. -- Regan, P. H. -- Walker, P. M.] Univ Surrey, Dept Phys, Guildford GU2 7XH, Surrey, England -- [Mitev, K. -- Rainovski, G.] Sofia Univ St Kliment Ohridski, Fac Phys, Sofia, Bulgaria -- [Ozben, C.] Istanbul Tech Univ, TR-80626 Istanbul, Turkey -- [Rudolph, D.] Lund Univ, Dept Phys, SE-22100 Lund, Sweden, Research Group: Information Management, CSNSM SNO, Centre de Spectrométrie Nucléaire et de Spectrométrie de Masse (CSNSM), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Centre de Sciences Nucléaires et de Sciences de la Matière (CSNSM), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique Nucléaire d'Orsay (IPNO), informatique, Institut de Physique Nucléaire de Lyon (IPNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Grand Accélérateur National d'Ions Lourds (GANIL), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Département de Physique Nucléaire (ex SPhN) (DPHN), Institut de Recherches sur les lois Fondamentales de l'Univers (IRFU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, CSNSM MECA, Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Subatomique et de Cosmologie (LPSC), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS), CSNSM INFOR, CSNSM ELEC, Matière Nucléaire, Institut de Physique des 2 Infinis de Lyon (IP2I Lyon), AGATA, Akkoyun, S., Algora, A., Alikhani, B., Ameil, F., De Angelis, G, Arnold, L., Astier, A., Ataç, A., Aubert, Y., Aufranc, C., Austin, A., Aydin, S., Azaiez, F., Badoer, S., Balabanski, D. L., Barrientos, D., Baulieu G., L, Baumann, R., Bazzacco, D., Beck, F. A., Beck, T., Bednarczyk, P., Bellato, M., Bentley, M. A., Benzoni, G., Berthier, R., Berti, L., Beunard, R., Lo Bianco, G., Birkenbach, B., Bizzeti, P. G., Bizzeti Sona, A. M., Le Blanc, F., Blasco, J. M., Blasi, N., Bloor, D., Boiano, C., Borsato, M., Bortolato, D., Boston, A. J., Boston, H. C., Bourgault, P., Boutachkov, P., Bouty, A., Bracco, A., Brambilla, S., Brawn, I. P., Brondi, Augusto, Broussard, S., Bruyneel, B., Bucurescu, D., Burrows, I., Bürger, A., Cabaret, S., Cahan, B., Calore, E., Camera, F., Capsoni, A., Carrió, F., Casati, G., Castoldi, M., Cederwall, B., Cercus J., L, Chambert, V., El Chambit, M., Chapman, R., Charles, L., Chavas, J., Clément, E., Cocconi, P., Coelli, S., Coleman Smith, P. J., Colombo, A., Colosimo, S., Commeaux, C., Conventi, D., Cooper, R. J., Corsi, A., Cortesi, A., Costa, L., Crespi, F. C. L., Cresswell, J. R., Cullen, D. M., Curien, D., Czermak, A., Delbourg, D., Depalo, R., Descombes, T., Désesquelles, P., Detistov, P., Diarra, C., Didierjean, F., Dimmock, M. R., Doan, Q. T., Domingo Pardo, C., Doncel, M., Dorangeville, F., Dosme, N., Drouen, Y., Duchêne, G., Dulny, B., Eberth, J., Edelbruck, P., Egea, J., Engert, T., Erduran, M. N., Ertürk, S., Fanin, C., Fantinel, S., Farnea, E., Faul, T., Filliger, M., Filmer, F., Finck, C., De France, G., Gadea, A., Gast, W., Geraci, A., Gerl, J., Gernhäuser, R., Giannatiempo, A., Giaz, A., Gibelin, L., Givechev, A., Goel, N., González, V., Gottardo, A., Grave, X., Grȩbosz, J., Griffiths, R., Grint, A. N., Gros, P., Guevara, L., Gulmini, M., Görgen, A., Ha, H. T. M., Habermann, T., Harkness, L. J., Harroch, H., Hauschild, K., He, C., Hernández Prieto, A., Hervieu, B., Hess, H., Hüyük, T., Ince, E., Isocrate, R., Jaworski, G., Johnson, A., Jolie, J., Jones, P., Jonson, B., Joshi, P., Judson, D. S., Jungclaus, A., Kaci, M., Karkour, N., Karolak, M., Kaşkaş, A., Kebbiri, M., Kempley, R. S., Khaplanov, A., Klupp, S., Kogimtzis, M., Kojouharov, I., Korichi, A., Korten, W., Kröll, T., Krücken, R., Kurz, N., Ky, B. Y., Labiche, M., Lafay, X., Lavergne, L., Lazarus, I. H., Leboutelier, S., Lefebvre, F., Legay, E., Legeard, L., Lelli, F., Lenzi, S. M., Leoni, S., Lermitage, A., Lersch, D., Leske, J., Letts, S. C., Lhenoret, S., Lieder, R. M., Linget, D., Ljungvall, J., Lopez Martens, A., Lotodé, A., Lunardi, S., Maj, A., Van Der Marel, J., Mariette, Y., Marginean, N., Marginean, R., Maron, G., Mather, A. R., Mȩczyński, W., Mendéz, V., Medina, P., Melon, B., Menegazzo, R., Mengoni, D., Merchan, E., Mihailescu, L., Michelagnoli, C., Mierzejewski, J., Milechina, L., Million, B., Mitev, K., Molini, P., Montanari, D., Moon, S., Morbiducci, F., Moro, RENATA EMILIA MARIA, Morrall, P. S., Möller, O., Nannini, A, Napoli, D. R., Nelson, L., Nespolo, M., Ngo, V. L., Nicoletto, M., Nicolini, R., Le Noa, Y., Nolan, P. J., Norman, M., Nyberg, J., Obertelli, A., Olariu, A., Orlandi, R., Oxley, D. C., Özben, C., Ozille, M., Oziol, C., Pachoud, E., Palacz, M., Palin, J., Pancin, J., Parisel, C., Pariset, P., Pascovici, G., Peghin, R., Pellegri, L., Perego, A., Perrier, S., Petcu, M., Petkov, P., Petrache, C., Pierre, E., Pietralla, N., Pietri, S., Pignanelli, M., Piqueras, I., Podolyak, Z., Le Pouhalec, P., Pouthas, J., Pugnére D., L, Pucknell, V. F. E., Pullia, A., Quintana, B., Raine, R., Rainovski, G., Ramina, L., Rampazzo, G., LA RANA, Giovanni, Rebeschini, M., Recchia, F., Redon N., L, Reese M., C, Reiter, P., Regan, P. H., Riboldi, S., Richer, M., Rigato, M., Rigby, S., Ripamonti, G., Robinson, A., Robin, J., Roccaz, J., Ropert, J. A., Rossé, B. l., Rossi Alvarez, C., Rosso, D., Rubio, B., Rudolph, D., Saillant, F., Şahin, E., Salomon, F., Salsac, M. D., Salt, J., Salvato, G., Sampson, J., Sanchis, E., Santos, C., Schaffner, H., Schlarb, M., Scraggs, D. P., Seddon, D., Şenyiǧit, M., Sigward, M. H., Simpson, G., Simpson, J., Slee, M., Smith, J. F., Sona, P., Sowicki, B., Spolaore, P., Stahl, C., Stanios, T., Stefanova, E., Stézowski, O., Strachan, J., Suliman, G., Söderström, P. A., Tain, J. L., Tanguy, S., Tashenov, S., Theisen, C. h., Thornhill, J., Tomasi, F., Toniolo, N., Touzery, R., Travers, B., Triossi, A., Tripon, M., Tun Lanoë, K. M. M., Turcato, M., Unsworth, C., Ur, C. A., Valiente Dobon, J. J., Vandone, V., Vardaci, Emanuele, Venturelli, R., Veronese, F., Veyssiere, C., Viscione, E., Wadsworth, R., Walker, P. M., Warr, N., Weber, C., Weisshaar, D., Wells, D., Wieland, O., Wiens A., U, Wittwer, G., Wollersheim, H. J., Zocca, F., Zamfir, N. V., Ziȩbliński, M., Zucchiatti, A., Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)-Centre de Sciences Nucléaires et de Sciences de la Matière (CSNSM), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), and 0-Belirlenecek

Subjects
Nuclear and High Energy Physics, Physics - Instrumentation and Detectors, Pulse-shape and gamma-ray tracking algorithms, FOS: Physical sciences, Semiconductor detector performance and simulations, Integrated circuit, [PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex], Tracking (particle physics), gamma-Ray tracking, 01 natural sciences, Pulse-shape and γ-ray tracking algorithms, law.invention, Data acquisition, law, 0103 physical sciences, ddc:530, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], Nuclear Experiment (nucl-ex), 010306 general physics, γ-Ray spectroscopy, Nuclear Experiment, Instrumentation, Digital signal processing, Event reconstruction, gamma-Ray spectroscopy, Physics, sezele, Spectrometer, Spectrometers, 010308 nuclear & particles physics, business.industry, Detector, AGATA, Digital signals, HPGe detectors, Pulse-shape, Ray tracking, HPGe detectors, Algorithms, Crystals, Germanium, Semiconductor detectors, Signal processing, Spectrometry, Tracking (position), γ-Ray tracking, Instrumentation and Detectors (physics.ins-det), AGATA, Física nuclear, business, Computer hardware
Abstract

WOS: 000300864200005, The Advanced GAmma Tracking Array (AGATA) is a European project to develop and operate the next generation gamma-ray spectrometer. AGATA is based on the technique of gamma-ray energy tracking in electrically segmented high-purity germanium crystals. This technique requires the accurate determination of the energy, time and position of every interaction as a gamma ray deposits its energy within the detector volume. Reconstruction of the full interaction path results in a detector with very high efficiency and excellent spectral response. The realisation of gamma-ray tracking and AGATA is a result of many technical advances. These include the development of encapsulated highly segmented germanium detectors assembled in a triple cluster detector cryostat, an electronics system with fast digital sampling and a data acquisition system to process the data at a high rate. The full characterisation of the crystals was measured and compared with detector-response simulations. This enabled pulse-shape analysis algorithms, to extract energy, time and position, to be employed. In addition, tracking algorithms for event reconstruction were developed. The first phase of AGATA is now complete and operational in its first physics campaign. In the future AGATA will be moved between laboratories in Europe and operated in a series of campaigns to take advantage of the different beams and facilities available to maximise its science output. The paper reviews all the achievements made in the AGATA project including all the necessary infrastructure to operate and support the spectrometer. (C) 2011 Elsevier B.V. All rights reserved., EU [RII3-CT-2004-506065]; German BMBF [06K-167, 06KY2051]; Swedish Research Council; Knut and Alice Wallenberg Foundation; UK EPSRC Engineering and Physical Sciences Research Council; UK STFC Science and Technology Facilities Council; AWE plc; Scientific and Technological Research Council of Turkey [106T055]; Ankara University [05B4240002]; Polish Ministry of Science and Higher Education [DPN/N190/AGATA/2009]; Spanish MICINN [FPA2008-06419, FPA2009-13377-C02-02]; Spanish Consolider-Ingenio Programme CPAN [CSD2007-00042]; Generalitat Valenciana [PROMETEO/2010/101]; MICINN, Spain; INFN, Italy [AIC10-D-000568]; Science and Technology Facilities Council [ST/F012039/1, ST/J000094/1, ST/F004192/1, ST/J000159/1, ST/I504940/1, NuSTAR, ST/F006950/1, ST/I504916/1, ST/G000670/1, ST/F004060/1, ST/J000051/1, ST/J000108/1, ST/G000670/1 NuSTAR, ST/I504959/1, ST/G000727/1, ST/F004052/1, ST/F004184/1], AGATA and this work is supported by the European funding bodies and the EU Contract RII3-CT-2004-506065, the German BMBF under Grants 06K-167 and 06KY2051, the Swedish Research Council and the Knut and Alice Wallenberg Foundation, UK EPSRC Engineering and Physical Sciences Research Council, UK STFC Science and Technology Facilities Council, AWE plc, Scientific and Technological Research Council of Turkey (Proj. nr. 106T055) and Ankara University (BAP Proj. nr. 05B4240002), the Polish Ministry of Science and Higher Education under Grant DPN/N190/AGATA/2009, the Spanish MICINN under grants FPA2008-06419 and FPA2009-13377-C02-02, the Spanish Consolider-Ingenio 2010 Programme CPAN (contract number CSD2007-00042) the Generalitat Valenciana under Grant PROMETEO/2010/101, and research performed in the frame of the GSI-IN2P3 collaboration agreement number 02-42. A. Gadea and E. Farnea acknowledge the support of MICINN, Spain, and INFN, Italy, through the AIC10-D-000568 bilateral action.

Published
2012
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41. Impact of influenza and pneumococcal vaccines on HIV persistence and immune dynamics during suppressive antiretroviral therapy.

Author

Gianella S, Anderson C, Chaillon A, Wells A, Porrachia M, Caballero G, Meneses M, Lonergan J, Woodworth B, Gaitan NC, Rawlings SA, Muttera L, Harkness L, Little SJ, May S, and Smith D

Subjects
Humans, Male, Female, Adult, Middle Aged, Double-Blind Method, Prospective Studies, Placebos administration & dosage, RNA, Viral blood, DNA, Viral blood, Anti-Retroviral Agents therapeutic use, Influenza, Human prevention & control, Influenza, Human immunology, Viral Load, HIV Infections drug therapy, HIV Infections immunology, Influenza Vaccines immunology, Influenza Vaccines administration & dosage, Pneumococcal Vaccines administration & dosage, Pneumococcal Vaccines immunology, Cross-Over Studies
Abstract

Objective: We sought to determine if standard influenza and pneumococcal vaccines can be used to stimulate HIV reservoirs during antiretroviral therapy (ART)., Design: A prospective, randomized, double-blinded, placebo-controlled, crossover trial of two clinically recommended vaccines (influenza and pneumococcal)., Methods: Persons with HIV on ART ( N  = 54) were enrolled in the clinical trial. Blood was collected at baseline and days 2,4,7,14, and 30 postimmunizations. Levels of cellular HIV RNA and HIV DNA were measured by ddPCR. Expression of immunological markers on T cell subsets was measured by flow cytometry. Changes in unspliced cellular HIV RNA from baseline to day 7 postinjection between each vaccine and placebo was the primary outcome., Results: Forty-seven participants completed at least one cycle and there were no serious adverse events related to the intervention. We observed no significant differences in the change in cellular HIV RNA after either vaccine compared with placebo at any timepoint. In secondary analyses, we observed a transient increase in total HIV DNA levels after influenza vaccine, as well as increased T cell activation and exhaustion on CD4 + T cells after pneumococcal vaccine., Conclusion: Clinically recommended vaccines were well tolerated but did not appear to stimulate the immune system strongly enough to elicit significantly noticeable HIV RNA transcription during ART.Clinicaltrials.gov identifier: NCT02707692., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)

Published
2024
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43. Graduate nurses' capability upon entering the workforce: An integrative review.

Author

Saghafi F, Bromley P, Guzys D, Harkness L, Phillips M, Mather C, Saunders A, Say R, Teare C, and Tori K

Subjects
Humans, Workforce, Education, Nursing, Graduate, Nurses
Abstract

Objectives: To clarify capability for work readiness in newly graduated registered nurses as viewed from the perspective of clinicians in practice, educators in tertiary institutions, and graduates., Design: Integrative review., Data Sources: Databases searched for peer-reviewed studies included PubMed, MEDLINE, ERIC, Campbell collaboration, Google Scholar, and Cochrane databases., Review Methods: Pragmatism informed this integrative review. The five-stage method described by Whittemore and Knafl was used to enable rigorous examination of the expected capability of graduate nurses. A comprehensive database search was conducted using PRISMA guidelines. Eighteen articles were appraised and analysed for this review. The capability concept was used as a framework for analysis., Results: Eighteen articles met the inclusion criteria. Findings revealed that although there is no definition for graduate nurses' work readiness, there is a common theme. Graduate nurses are expected to have broad theoretical knowledge (knowing) along with practical knowledge (doing). They are also expected to demonstrate integrity, honesty, respect, compassion, and a moral compass. A list of personal attributes and organisational acumen was also reflective of graduate readiness upon entering the workforce and identified as necessary capabilities for graduates., Conclusions: A picture of the perfect employee is illustrated in the definition of work readiness by the participants of the original studies. Yet there is a lack of stakeholder consensus on the capabilities expected from a graduate nurse., Competing Interests: Declaration of competing interest None., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Published
2023
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44. Development and Initial Evaluation of a Nurse-Led Healthcare Clinic for Homeless and At-Risk Populations in Tasmania, Australia: A Collaborative Initiative.

Author

Bennett-Daly G, Unwin M, Dinh H, Dowlman M, Harkness L, Laidlaw J, and Tori K

Subjects
Adult, Australia, Health Services Accessibility, Humans, Male, Tasmania, Ill-Housed Persons, Nurse's Role
Abstract

People who are homeless experience significantly poorer health than the general population and often face multifaceted challenges engaging with public healthcare services. Mission Health Nurse-led Clinic (MHNC) was established in 2019 to meet the healthcare needs of this marginalised population in Launceston, Tasmania. This study examines barriers to healthcare access amongst individuals who experience homelessness, client and staff perceptions of the MHNC services and explored opportunities for service expansion. Descriptive statistics were drawn from administrative data, and all interviews were thematically analysed. A total of 426 presentations were reported for 174 individuals experiencing homelessness over 26 months. The median client age was 42 years and 60.9% were male; A total of 38.5% were homeless or lived in a supported accommodation. The predominant reasons for clinic visits included prescription requests (25.3%) and immunisations (20.1%). A total of 10 clients and 5 City Mission staff were interviewed with three themes emerging from the findings: personal vulnerability, disconnectedness and acceptability of the MHNC. The MHNC services were reported to be highly appreciated by all clients. Mental health and allied health, extra operating hours and maintaining the flexibility of walk-in appointments were suggested as expansion areas for the service and were highlighted as ways to increase engagement for improved health outcomes. Continued partnerships with interprofessional primary healthcare providers would contribute to addressing unmet healthcare needs in this vulnerable population.

Published
2021
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45. Nutritional Gaps and Supplementation in the First 1000 Days.

Author

Beluska-Turkan K, Korczak R, Hartell B, Moskal K, Maukonen J, Alexander DE, Salem N, Harkness L, Ayad W, Szaro J, Zhang K, and Siriwardhana N

Subjects
Child, Preschool, Female, Health Education, Health Knowledge, Attitudes, Practice, Humans, Infant, Infant, Newborn, Maternal Nutritional Physiological Phenomena, Nutrition Policy, Pregnancy, Prenatal Care, Dietary Supplements, Nutritional Requirements
Abstract

Optimized nutrition during the first 1000 days (from conception through the 2nd birthday) is critical for healthy development and a healthy life for the newborn. Pregnancy and the postpartum period are accompanied by physiological changes, increased energy needs, and changing requirements in the nutrients critical for optimal growth and development. Infants and toddlers also experience physiological changes and have specific nutritional needs. Food and nutrition experts can provide women of childbearing age with adequate dietary advice to optimize nutrition, as well as guidance on selecting appropriate dietary supplements. Considering the approaching 2020-2025 Dietary Guidelines for Americans (DGA) will be making specific recommendations for children, it is important to provide accurate scientific information to support health influencers in the field of nutrition. The purpose of this review is to summarize the nutrition and supplementation literature for the first 1000 days; to highlight nutritional and knowledge gaps; and to educate nutrition influencers to provide thoughtful guidance to mothers and families. Optimal nutrition during pregnancy through early childhood is critical for supporting a healthy life. Nutrition influencers, such as dietitians, obstetricians/gynecologists, and other relevant health professionals, should continue guiding supplement and food intake and work closely with expectant families and nutrition gatekeepers.

Published
2019
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46. Fibronectin-conjugated thermoresponsive nanobridges generate three dimensional human pluripotent stem cell cultures for differentiation towards the neural lineages.

Author

Harkness L, Chen X, Jia Z, Davies AM, Monteiro M, Gray P, and Pera M

Subjects
Cell Line, Human Embryonic Stem Cells cytology, Humans, Neural Stem Cells cytology, Cell Differentiation, Fibronectins chemistry, Human Embryonic Stem Cells metabolism, Nanostructures chemistry, Neural Stem Cells metabolism
Abstract

Production of 3-dimensional neural progenitor cultures from human pluripotent stem cells offers the potential to generate large numbers of cells. We utilised our nanobridge system to generate 3D hPSC aggregates for differentiation towards the neural lineage, and investigate the ability to passage aggregates while maintaining cells at a stem/progenitor stage. Over 38 days, aggregate cultures exhibited upregulation and maintenance of neural-associated markers and demonstrated up to 10 fold increase in cell number. Aggregates undergoing neural induction in the presence or absence of nanobridges demonstrated no differences in marker expression, proliferation or viability. However, aggregates formed without nanobridges were statistically significantly fewer and smaller by passage 3. Organoids, cultured from aggregates, and treated with retinoic acid or rock inhibitor demonstrated terminal differentiation as assessed by immunohistochemistry. These data demonstrate that nanobridge 3D hPSC can differentiate to neural stem/progenitor cells, and be maintained at this stage through serial passaging and expansion., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2019
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47. Neoplastic Transformation of Human Mesenchymal Stromal Cells Mediated via LIN28B.

Author

Vishnubalaji R, Elango R, Al-Toub M, Manikandan M, Al-Rikabi A, Harkness L, Ditzel N, Atteya M, Hamam R, Alfayez M, Aldahmash A, Kassem M, and Alajez NM

Subjects
Antibiotics, Antineoplastic pharmacology, Antibiotics, Antineoplastic therapeutic use, Cell Line, Cell Movement genetics, Cell Proliferation genetics, Cohort Studies, Datasets as Topic, Disease-Free Survival, Doxorubicin pharmacology, Doxorubicin therapeutic use, Drug Resistance, Neoplasm genetics, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Kaplan-Meier Estimate, Mesenchymal Stem Cells metabolism, Sarcoma drug therapy, Sarcoma mortality, Sarcoma pathology, Up-Regulation, Xenograft Model Antitumor Assays, Cell Transformation, Neoplastic genetics, HMGA2 Protein genetics, Mesenchymal Stem Cells pathology, MicroRNAs metabolism, RNA-Binding Proteins genetics, Sarcoma genetics
Abstract

Bone marrow stromal (Mesenchymal) stem cells (MSCs) are multipotent bone cells capable of differentiating into mesoderm-type cells, such as osteoblasts and adipocytes. Existing evidence suggests that transformation of MSCs gives rise to sarcoma. In order to identify the molecular mechanism leading to spontaneous transformation of human bone marrow MSCs (hBMSCs), we performed comprehensive microRNA (miRNA) and mRNA profiling in the transformed hBMSC-Tum line compared to the parental clone. As a result, we identified multiple dysregulated molecular networks associated with the hBMSC transformed phenotype. LIN28B was upregulated 177.0-fold in hBMSC-Tum, which was associated with marked reduction in LET-7 expression and upregulated expression of its target HMGA2. Targeted depletion of LIN28B or exogenous expression of LET-7b suppressed hBMSC-Tum proliferation, colony formation, and migration. On the other hand, forced expression of LIN28B promoted malignant transformation of parental hBMSC cells as shown by enhanced in vitro colony formation, doxorubicin resistance, and in vivo tumor formation in immunocompromised mice. Analysis of LIN28B and HMGA2 expression levels in cohorts from The Cancer Genome Atlas sarcoma dataset revealed a strong inverse-relationship between elevated expression and overall survival (OS) in 260 patients (p = 0.005) and disease-free survival (DFS) in 231 patients (p = 0.02), suggesting LIN28B and HMGA2 are important regulators of sarcoma biology. Our results highlight an important role for the LIN28B/LET-7 axis in human sarcoma pathogenesis and suggest that the therapeutic targeting of LIN28B may be relevant for patients with sarcoma.

Published
2019
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48. Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential.

Author

Harkness L, Chen X, Gillard M, Gray PP, and Davies AM

Subjects
Cell Culture Techniques, Cell Line, Cluster Analysis, Cytoskeleton metabolism, Ectoderm cytology, Embryoid Bodies, Endoderm cytology, Gene Expression Profiling, Humans, Mesoderm cytology, Microscopy, Fluorescence, Transcription, Genetic, Cell Differentiation, Cell Lineage, Culture Media chemistry, Human Embryonic Stem Cells cytology, Pluripotent Stem Cells cytology
Abstract

Undifferentiated human embryonic stem cells have a distinct morphology (hESC). Changes in cell morphology during culture can be indicative of differentiation. hESC, maintained in diverse medias, demonstrated alterations in morphological parameters and subsequent alterations in underlying transcript expression and lineage differentiation. Analysis of morphological parameters showed distinct and significant differences between the undefined, less defined and Xeno-free medias while still maintaining pluripotency markers. This suggested that the less defined media may be creating dynamic instability in the cytoskeleton, with the cytoskeleton becoming more stabilised in the Xeno-free media as demonstrated by smaller and rounder cells. Examination of early lineage markers during undirected differentiation using d5 embryoid bodies demonstrated increased mesodermal lineage preference as compared to endodermal or ectoderm in cells originally cultured in Xeno-free media. Undefined media showed preference for mesoderm and ectoderm lineages, while less defined media (BSA present) demonstrated no preference. These data reveal that culture media may produce fundamental changes in cell morphology which are reflected in early lineage differentiation choice., Competing Interests: The authors have declared that no competing interests exist.

Published
2019
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49. TAFA2 Induces Skeletal (Stromal) Stem Cell Migration Through Activation of Rac1-p38 Signaling.

Author

Jafari A, Isa A, Chen L, Ditzel N, Zaher W, Harkness L, Johnsen HE, Abdallah BM, Clausen C, and Kassem M

Subjects
Adipocytes metabolism, Adipocytes pathology, Animals, Cell Differentiation drug effects, Cell Proliferation drug effects, Chemokines, CC metabolism, Disease Models, Animal, Hip Fractures metabolism, Hip Fractures pathology, Humans, Mesenchymal Stem Cells pathology, Mice, Neuropeptides metabolism, Osteoblasts metabolism, Osteoblasts pathology, Cell Movement drug effects, Chemokines, CC pharmacology, MAP Kinase Signaling System drug effects, Mesenchymal Stem Cells metabolism, p38 Mitogen-Activated Protein Kinases metabolism, rac1 GTP-Binding Protein metabolism
Abstract

Understanding the mechanisms regulating recruitment of human skeletal (stromal or mesenchymal) stem cells (hMSC) to sites of tissue injury is a prerequisite for their successful use in cell replacement therapy. Chemokine-like protein TAFA2 is a recently discovered neurokine involved in neuronal cell migration and neurite outgrowth. Here, we demonstrate a possible role for TAFA2 in regulating recruitment of hMSC to bone fracture sites. TAFA2 increased the in vitro trans-well migration and motility of hMSC in a dose-dependent fashion and induced significant morphological changes including formation of lamellipodia as revealed by high-content-image analysis at single-cell level. Mechanistic studies revealed that TAFA2 enhanced hMSC migration through activation of the Rac1-p38 pathway. In addition, TAFA2 enhanced hMSC proliferation, whereas differentiation of hMSC toward osteoblast and adipocyte lineages was not altered. in vivo studies demonstrated transient upregulation of TAFA2 gene expression during the inflammatory phase of fracture healing in a closed femoral fracture model in mice, and a similar pattern was observed in serum levels of TAFA2 in patients after hip fracture. Finally, interleukin-1β was found as an upstream regulator of TAFA2 expression. Our findings demonstrate that TAFA2 enhances hMSC migration and recruitment and thus is relevant for regenerative medicine applications. Stem Cells 2019;37:407-416., (© 2018 The Authors. Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press 2018.)

Published
2019
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50. Canine CD117-Specific Antibodies with Diverse Binding Properties Isolated from a Phage Display Library Using Cell-Based Biopanning.

Author

Alfaleh MA, Arora N, Yeh M, de Bakker CJ, Howard CB, Macpherson P, Allavena RE, Chen X, Harkness L, Mahler SM, and Jones ML

Abstract

CD117 (c-Kit) is a tyrosine kinase receptor that is overexpressed in multiple dog tumors. There is 100% homology between the juxtamembrane domain of human and canine CD117, and many cancer-causing mutations occur in this region in both species. Thus, CD117 is an important target for cancer treatment in dogs and for comparative oncology studies. Currently, there is no monoclonal antibody (mAb) specifically designed to target the exposed region of canine CD117, although there exist some with species cross-reactivity. We panned a naïve phage display library to isolate antibodies against recombinant CD117 on whole cells. Several mAbs were isolated and were shown to bind recombinant canine CD117 at low- to sub-nanomolar affinity. Additionally, binding to native canine CD117 was confirmed by immunohistochemistry and by flow cytometry. Competitive binding assays also identified mAbs that competed with the CD117 receptor-specific ligand, the stem cell factor (SCF). These results show the ability of our cell-based biopanning strategy to isolate a panel of antibodies that have varied characteristics when used in different binding assays. These in vitro/ex vivo assessments suggest that some of the isolated mAbs might be promising candidates for targeting overexpressed CD117 in canine cancers for different useful applications.

Published
2019
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