Search

Abstract The release of textile dyes from the industries to the environment has become a significant health issue to humans and their surroundings, where a large number of dyes are wasted to the nearby water during the dyeing process. Therefore, the practice of the utilization of biosynthesized nanoparticles from numerous biological systems has been described, out of which plant material is considered the most appropriate method. The usage of plant material not only makes the procedure ecological but also their abundance makes it more cost-effective. The present study aims to biologically produce selenium nanoparticle using Mucuna pruriens seed powder and apply it in the degradation of dyes and removal of pathogenic bacterial cultures from pathogen-containing lake water using an optimum concentration of selenium nanoparticles. The production of selenium nanoparticles was characterized via visual coloration from colorless to brown solution which was checked using UV–Visible spectrophotometer, the crystalline structure was analyzed using X-ray diffraction, the morphology was analyzed with transmission electron microscopy, scanning electron microscopy, dynamic light scattering, which was in the range of 90–120 nm, with stable spherical monodisperse characteristics. The surface potential was checked using zeta potential, while the Atomic Force Microscopy gave information on the roughness of the nanoparticles, and the presence of –OH, −COO, aromatic groups were confirmed with Fourier Transform Infrared spectroscopy. The biosynthesized nanoparticles were then analyzed for its environmental applications, like degradation of dyes from industrial wastes and checking its antimicrobial activities.

Anti-inflammatory activity possessing bio-compounds were identified from Kalanchoe pinnata methanolic leaf extract using Gas chromatography-mass spectrometry (GC-MS) analysis and their anti-inflammatory potential was checked using inhibition of albumin denaturation assay, anti-proteinase activity assay, and heat and hypotonicity induced hemolysis assay. GC-MS analysis revealed the presence of aldehydes, ketones, carboxylic acid in the crude. Antioxidant activity analysis using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay revealed the half maximal inhibitory concentration (IC50) value of methanol crude leaf extract as 100 μg/mL. The biocompatibility of the methanol extract was determined using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay.The anti-inflammatory property of identified bio-compounds was also validated using a computational approach. Drug likelihood properties of phyto-compounds were analyzed by predicting the absorption, distribution, metabolism, and excretion (ADMET) score and the bioactivity score. Six chemical compounds with excellent drug relevant properties and availability were selected for a molecular docking study versus a common inflammatory molecular target COX-2 protein. The present study showed that anti-inflammatory properties of phyto-compounds from methanol crude leaf extract of Kalanchoe pinnata can be exploited for novel drug design to treat inflammatory disorders. Keywords: Inflammation, Kalanchoe pinnata, In-silico, GCMS, Cytokines, Anti-oxidant

Zinc oxide, an established inorganic metal oxide in nanoparticles form exhibits tremendous anti-bacterial activity. The present study focuses on determining the anti-bacterial activity of green synthesized zinc oxide nanoparticles (ZnO NPs). Results clearly validate the effective synthesis of spherical shaped nanoparticles with average size range of 60-80 nm. SEM and EDAX data buttresses the results obtained by XRD pattern in terms of size and purity. ZnO NPs exhibited dose-dependent anti-bacterial activity against Escherichia coli ( E. coli) and the IC 50 value was calculated to be around 20 μg/mL. Growth kinetics study was conducted in the presence of nanoparticles which demonstrated the bacteriostatic effect of ZnO NPs. The study recommends the potential use of ZnO NPs in industries like food, pharmaceutical, agriculture, cosmetic industries for its anti-bacterial activity.