1. Detection of QTLs for salt tolerance in Asian barley (Hordeum vulgare L.) by association analysis with SNP markers
- Author
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Kazuhiro Sato, Moncef Harrabi, Hanen Sbei, Tariq Shehzad, and Kazutoshi Okuno
- Subjects
Hordeum vulgare L ,salt tolerance ,Chlorosis ,biology ,fungi ,food and beverages ,Single-nucleotide polymorphism ,Plant Science ,Quantitative trait locus ,biology.organism_classification ,Research Papers ,leaf injury score ,SNP markers ,Horticulture ,Dry weight ,Seedling ,Botany ,Shoot ,quantitative trait loci ,Genetics ,Hordeum vulgare ,salt tolerance index ,Association mapping ,association mapping ,Agronomy and Crop Science - Abstract
Two hundred ninetysix Asian barley (Hordeum vulgare L.) accessions were assessed to detect QTLs underlying salt tolerance by association analysis using a 384 single nucleotide polymorphism (SNP) marker system. The experiment was laid out at the seedling stage in a hydroponic solution under control and 250 mM NaCl solution with three replications of four plants each. Salt tolerance was assessed by leaf injury score (LIS) and salt tolerance indices (STIs) of the number of leaves (NL), shoot length (SL), root length (RL), shoot dry weight (SDW) and root dry weight (RDW). LIS was scored from 1 to 5 according to the severity of necrosis and chlorosis observed on leaves. There was a wide variation in salt tolerance among Asian barley accessions. LIS and STI (SDW) were the most suitable traits for screening salt tolerance. Association was estimated between markers and traits to detect QTLs for LIS and STI (SDW). Seven significant QTLs were located on chromosomes 1H (2 QTLs), 2H (2 QTLs), 3H (1 QTL), 4H (1 QTL) and 5H (1 QTL). Five QTLs were asso ciated with LIS and 2 QTLs with STI (SDW). Two QTLs associated with LIS were newly identified on chromosomes 3H and 4H.
- Published
- 2014