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In this paper, we discuss a higher-order convergent numerical method for a two-parameter singularly perturbed differential equation with a discontinuous convection coefficient and a discontinuous source term. The presence of perturbation parameters generates boundary layers, and the discontinuous terms produce interior layers on both sides of the discontinuity. In order to obtain a higher-order convergent solution, a hybrid monotone finite difference scheme is constructed on a piecewise uniform Shishkin mesh, which is adapted inside the boundary and interior layers. On this mesh (including the point of discontinuity), the present method is almost second-order parameter-uniform convergent. The current scheme is compared with the standard upwind scheme, which is used at the point of discontinuity. The numerical experiments based on the proposed scheme show higher-order (almost second-order) accuracy compared to the standard upwind scheme, which provides almost first-order parameter-uniform convergence.

ABSTRACT: Asparagopsis taxiformis inhibits ruminal methane (CH4) production due to its bromoform (CHBr3) content. The immersion of A. taxiformis in edible vegetable oils allows the extraction and stabilization of the highly volatile CHBr3 in the oil phase. The objectives of this study were to explore the effects of adding sunflower oils with increasing concentrations of CHBr3 on in vitro ruminal methanogenesis and biohydrogenation. Five batches of 48-h in vitro incubations were performed in 14 fermentation bottles, using rumen inocula collected shortly after the slaughter of young crossbred bulls and 1 g of dry matter (DM) from a total diet of mixed feed without added oil (control) or with 60 μL of sunflower oil per gram of DM as the substrate. The treatments were the CHBr3 content in the oil added: 0 μg (B0), 25 μg (B25), 50 μg (B50), 75 μg (B75), 100 μg (B100), and 150 μg (B150) of CHBr3 per gram of substrate DM. Organic matter (OM) degradability, total gas, CH4, volatile fatty acids (VFA), long-chain fatty acids, and dimethyl acetals (DMA) were analyzed at the end of each incubation. Data were analyzed with a model considering the treatments as the fixed effect and the run as a random block and using orthogonal contrasts. Degradability of OM was higher in the control group and was unaffected by CHBr3 concentration. Total gas production per gram of degraded OM was unaffected by treatments and averaged 205 ± 29.8 mL/g. Methane (mL) production decreased linearly with increasing CHBr3 concentrations, with 33%, 47%, and 87% reductions for B75, B100, and B150, respectively. Total VFA concentration was unaffected by oil inclusion but was reduced by 20% in CHBr3-containing treatments, although without any dose-response pattern. The molar percentage of acetate decreased linearly, whereas propionate and butyrate increased linearly with the increasing CHBr3 dosage. Including oil in the diet decreased the branched-chain fatty acids and DMA content. Increasing CHBr3 concentrations did not affect branched-chain fatty acids, but linearly increased most of the identified DMA. Adding oil to the control diet increased the 18:2n-6, whereas increasing the concentration of CHBr3 had no effect on 18:2n-6 but decreased linearly the 18:0 and increased the trans-18:1 isomers. The results obtained provide evidence that oil immersions of A. taxiformis can successfully inhibit ruminal production of CH4 in vitro at doses of 100 and 150 μg/g DM, and simultaneously modulate biohydrogenation.

The red seaweed Asparagopsis taxiformis has a potent antimethanogenic effect, which has been proven both in vitro and in vivo. Vegetable oil immersions of this seaweed (hereafter Bromoil) help stabilise the bromoform (CHBr3) responsible for its antimethanogenic effect. We evaluate the effects of increasing the levels of CHBr3 in lamb diets on growth performance, methane (CH4) production, animal health and meat quality. Twenty-four Merino Branco ram lambs were fed a ground complete compound feed, supplemented with 50 mL/kg DM of sunflower oil with different CHBr3 content. The treatments were defined by the CHBr3 doses in the oil: 0 mg (control – B0), 15 mg (B15), 30 mg (B30) and 45 mg (B45) of CHBr3 per kg of feed DM. The feed was prepared daily by mixing Bromoil with the compound feed. At the end of the experiment, the lambs were sacrificed, the ruminal content was collected for in vitro fermentation to evaluate CH4 production and organic matter (OM) degradability, and the rumen mucosa was sampled for histological examination. Meat samples were collected for chemical composition and CHBr3 analysis. The half-life of CHBr3 in the air-exposed feed was 3.98 h making it very difficult to establish the practiced level of CHBr3 supplementation. Lambs−fed treatments B30 and B45 decreased DM intake by up to 28%. Average daily gain was also reduced due to CHBr3 supplementation, with B45 showing results 40% lower than B0. DM feed conversion ratio was similar for all treatments. The degradability of OM, the volume of total gas and of gas without CH4 were unaffected by the experimental treatments, evaluated by the in vitro method. However, the volume of CH4 decreased by up to 75% for treatments above 30 mg/kg DM, while the yield of CH4/g OM degraded was reduced by up to 78% with treatments above 30 mg/kg DM. Meat chemical composition was not affected by Bromoil supplementation and no traces of CHBr3 were found in meat samples. During this experiment, the animals presented normal health and behaviour. However, postslaughter examination of the rumen showed distinct lesions on the ventral region of the rumen mucosa of animals supplemented with Bromoil. These lesions were more severe in the animals receiving treatments B30 and B45. This research determined that although concentrations of CHBr3 in the diet above 30 mg/kg DM helped to reduce CH4 emissions, it negatively affected the performance and rumen wall.

Background The long-term outcomes of COVID-19 hospitalisation in individuals with pre-existing airway diseases are unknown. Methods Adult participants hospitalised for confirmed or clinically suspected COVID-19 and discharged between 5 March 2020 and 31 March 2021 were recruited to the Post-hospitalisation COVID-19 (PHOSP-COVID) study. Participants attended research visits at 5 months and 1 year post discharge. Clinical characteristics, perceived recovery, burden of symptoms and health-related quality of life (HRQoL) of individuals with pre-existing airway disease (i.e., asthma, COPD or bronchiectasis) were compared to the non-airways group. Results A total of 615 out of 2697 (22.8%) participants had a history of pre-existing airway diseases (72.0% diagnosed with asthma, 22.9% COPD and 5.1% bronchiectasis). At 1 year, the airways group participants were less likely to feel fully recovered (20.4% versus 33.2%, p

Summary: Background: The scale of COVID-19 and its well documented long-term sequelae support a need to understand long-term outcomes including frailty. Methods: This prospective cohort study recruited adults who had survived hospitalisation with clinically diagnosed COVID-19 across 35 sites in the UK (PHOSP-COVID). The burden of frailty was objectively measured using Fried's Frailty Phenotype (FFP). The primary outcome was the prevalence of each FFP group—robust (no FFP criteria), pre-frail (one or two FFP criteria) and frail (three or more FFP criteria)—at 5 months and 1 year after discharge from hospital. For inclusion in the primary analysis, participants required complete outcome data for three of the five FFP criteria. Longitudinal changes across frailty domains are reported at 5 months and 1 year post-hospitalisation, along with risk factors for frailty status. Patient-perceived recovery and health-related quality of life (HRQoL) were retrospectively rated for pre-COVID-19 and prospectively rated at the 5 month and 1 year visits. This study is registered with ISRCTN, number ISRCTN10980107. Findings: Between March 5, 2020, and March 31, 2021, 2419 participants were enrolled with FFP data. Mean age was 57.9 (SD 12.6) years, 933 (38.6%) were female, and 429 (17.7%) had received invasive mechanical ventilation. 1785 had measures at both timepoints, of which 240 (13.4%), 1138 (63.8%) and 407 (22.8%) were frail, pre-frail and robust, respectively, at 5 months compared with 123 (6.9%), 1046 (58.6%) and 616 (34.5%) at 1 year. Factors associated with pre-frailty or frailty were invasive mechanical ventilation, older age, female sex, and greater social deprivation. Frail participants had a larger reduction in HRQoL compared with before their COVID-19 illness and were less likely to describe themselves as recovered. Interpretation: Physical frailty and pre-frailty are common following hospitalisation with COVID-19. Improvement in frailty was seen between 5 and 12 months although two-thirds of the population remained pre-frail or frail. This suggests comprehensive assessment and interventions targeting pre-frailty and frailty beyond the initial illness are required. Funding: UK Research and Innovation and National Institute for Health Research.

Abstract Foodborne bacteria like Escherichia coli threaten global food security, necessitating affordable, on-site detection methods, especially in resource-limited settings. This study optimized loop-mediated isothermal amplification (LAMP) integrated with peroxidase-mimicking G-quadruplex DNA structures (DNAzyme), termed DNAzyme-LAMP which was designed to incorporate two different catalytic DNAzymes per amplification unit, enabling colorimetric detection of E. coli in leafy vegetables and milk samples. Additionally, we introduce a novel electrochemical method that enhances analytical sensitivity. The optimized DNAzyme-LAMP achieved a detection limit below 6.3 CFU per reaction or 0.1 aM gene copies. This system lays the groundwork for the development of on-site biosensors and can be adapted for detecting other foodborne pathogens.

Recientemente, la modificación de aceites vegetales para obtener ésteres metílicos de ácidos grasos (FAMEs) o biodiesel ha emergido como una alternativa para la sustitución de los derivados del petróleo, esto debido a los problemas ambientales y de salud que genera su uso. Debido a su estructura química es posible epoxidar estas moléculas y usarlas directamente para producir plastificantes o lubricantes. Sin embargo, éstas también pueden ser sujetas a modificaciones para mejorar sus propiedades y el de servir como intermediarias para la síntesis de poliuretanos. Puesto que los métodos convencionales para la producción de epóxidos también son una fuente potencial de contaminación, se ha sugerido el uso de catalizadores enzimáticos como una alternativa sostenible o “Verde” para su preparación, ya que permiten obtener productos con alta pureza y mejores rendimientos. Este artículo presenta una revisión de la literatura disponible centrándose en la epoxidación enzimática de los FAMEs, así como sus principales aplicaciones.

Objective The coronavirus disease 2019 (COVID-19) has placed unprecedented challenges on the world and the medical community. It is transmitted through droplets, contact, the fecal-oral route, and airborne transmission under certain conditions that allow droplets to combine with air particles to form an aerosol. Viral loads are higher in the nasal area and similar in symptomatic and asymptomatic patients. Medical situations have been classified into high and low risk of generating aerosols. Most procedures and surgery in otolaryngology correspond to high-risk medical situations. This review aims to gather the vast amount of available information and generate recommendations for different surgical procedures according to aerosolization risk and COVID-19 status, with use of specific personal protective equipment in each case. Data Sources PubMed, MEDLINE, and Embase. Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, and Food and Drug Administration. Review Methods We conducted a review on the literature on personal protective equipment for otolaryngologic surgery and surgical indication restrictions during the COVID-19 pandemic. Conclusions SARS-CoV-2 is an easily transmitted virus. Asymptomatic and symptomatic patients with COVID-19 present an upper airway high viral load, conferring otolaryngologic procedures a high risk of aerosolization. Surgical procedures must be categorized according to aerosolization risk and the possibility of COVID-19 diagnosis, according to use of personal protective equipment. Implications for Practice This review contributes to scientific knowledge regarding the detailed description of protective personal equipment and, most important, surgical recommendations to reduce the risk of infection in the otolaryngology community during the COVID-19 pandemic.

Reducing household energy consumption is one of the most important strategies used to decrease fossil fuel consumption and greenhouse gases emissions, and to encourage renewable energy utilization. Most energy conservation strategies in the domestic sector are aimed at preferential loans, i.e., purchasing renewable electricity or to improve the efficiency of home appliances, such as air conditioning and lighting. However, despite the relative economic successes of these technologies, they have not had expected impacts in regard to energy consumption. In this work, the authors analyzed the consumption patterns of two equivalent households—one was adapted with improved thermal insulation and a 1.2 kW photovoltaic system to reduce consumption from the electrical grid. The results show that dwellings where no improvements were made registered lower electric energy consumption, due the fact that users were aware that no strategy had been implemented, and its consumption; hence, electricity payments depended solely on one’s attention over the electronic device operations. On the other hand, energy conservation strategies in households promotes confident and relaxed attitudes toward the use of energy, leading to lower energy billings, but a higher gross energy consumption.

Surface-active compounds (SACs) of microbial origin are an active group of biomolecules with potential use in the formulation of emulsions. In this sense, the present study aimed to isolate and select yeasts from fruits that could produce SACs for essential oil emulsions. The Candida krusei M4CK was isolated from the Byrsonima crassifolia fruit to make SACs. This emulsification activity (E24) was equal to or greater 50% in all carbon sources, such as olive oil, sunflower oil, kerosene, hexane, and hexadecane. E24 followed exponential growth according to the growth phase. The stability of emulsions was maintained over a wide range of temperatures, pH, and salinity. The OMBE4CK (melaleuca essential oil emulsion) had better and more significant inhibitory potential for biofilm reduction formation. In addition, bioemulsifier BE4CK alone on Escherichia coli and Pseudomonas aeruginosa biofilm showed few effective results, while there was a significant eradication for Staphylococcus aureus biofilms. The biofilms formed by S. aureus were eradicated in all concentrations of OMBE4CK. At the same time, the preformed biofilm by E. coli and P. aeruginosa were removed entirely at concentrations of 25 mg/mL, 12.5 mg/mL, and 6.25 mg/mL. The results show that the bioemulsifier BE4CK may represent a new potential for antibiofilm application. [ABSTRACT FROM AUTHOR]

Many fields, such as drought-risk assessment or reservoir management, can benefit from long-range streamflow forecasts. Climatology has long been used in long-range streamflow forecasting. Conditioning methods have been proposed to select or weight relevant historical time series from climatology. They are often based on general circulation model (GCM) outputs that are specific to the forecast date due to the initialisation of GCMs on current conditions. This study investigates the impact of conditioning methods on the performance of seasonal streamflow forecasts. Four conditioning statistics based on seasonal forecasts of cumulative precipitation and the standardised precipitation index were used to select relevant traces within historical streamflows and precipitation respectively. This resulted in eight conditioned streamflow forecast scenarios. These scenarios were compared to the climatology of historical streamflows, the ensemble streamflow prediction approach and the streamflow forecasts obtained from ECMWF System 4 precipitation forecasts. The impact of conditioning was assessed in terms of forecast sharpness (spread), reliability, overall performance and low-flow event detection. Results showed that conditioning past observations on seasonal precipitation indices generally improves forecast sharpness, but may reduce reliability, with respect to climatology. Conversely, conditioned ensembles were more reliable but less sharp than streamflow forecasts derived from System 4 precipitation. Forecast attributes from conditioned and unconditioned ensembles are illustrated for a case of drought-risk forecasting: the 2003 drought in France. In the case of low-flow forecasting, conditioning results in ensembles that can better assess weekly deficit volumes and durations over a wider range of lead times.

Abstract Roads have a myriad of negative effects on biodiversity, ultimately threatening the persistence of populations. In this Perspective we call attention to an extreme example, where the entire current geographic range of the endangered golden lion tamarin (Leontopithecus rosalia, GLT) is bisected by a major highway that is being widened to four lanes. We believe that the planned mitigation actions are not enough to reduce the expected increase of barrier effects and road mortality. These impacts may lead to a sequence of cascading effects that could jeopardize the conservation actions that prevented the extinction of GLTs three decades ago. We identify specific road sections along the highway and accompanying paved roads in the region that if equipped with tailored over passages would greatly reduce the road barrier effects. We also highlight areas where reforestation efforts could be extended in order to help reestablishing the connectivity between GLT habitat areas. We suggest that the working group integrating key decision makers and stakeholders, including the Non‐governmental organization leading the conservation efforts, partner universities, national road and environmental agencies, and the road construction company, to implement and to monitor the complementary road passages to improve connectivity of GLT habitat, and consequently to ensure the species' survival.

Meteorological centres make sustained efforts to provide seasonal forecasts that are increasingly skilful, which has the potential to benefit streamflow forecasting. Seasonal streamflow forecasts can help to take anticipatory measures for a range of applications, such as water supply or hydropower reservoir operation and drought risk management. This study assesses the skill of seasonal precipitation and streamflow forecasts in France to provide insights into the way bias correcting precipitation forecasts can improve the skill of streamflow forecasts at extended lead times. We apply eight variants of bias correction approaches to the precipitation forecasts prior to generating the streamflow forecasts. The approaches are based on the linear scaling and the distribution mapping methods. A daily hydrological model is applied at the catchment scale to transform precipitation into streamflow. We then evaluate the skill of raw (without bias correction) and bias-corrected precipitation and streamflow ensemble forecasts in 16 catchments in France. The skill of the ensemble forecasts is assessed in reliability, sharpness, accuracy and overall performance. A reference prediction system, based on historical observed precipitation and catchment initial conditions at the time of forecast (i.e. ESP method) is used as benchmark in the computation of the skill. The results show that, in most catchments, raw seasonal precipitation and streamflow forecasts are often more skilful than the conventional ESP method in terms of sharpness. However, they are not significantly better in terms of reliability. Forecast skill is generally improved when applying bias correction. Two bias correction methods show the best performance for the studied catchments, each method being more successful in improving specific attributes of the forecasts: the simple linear scaling of monthly values contributes mainly to increasing forecast sharpness and accuracy, while the empirical distribution mapping of daily values is successful in improving forecast reliability.

Probabilistic hydro-meteorological forecasts have over the last decades been used more frequently to communicate forecast uncertainty. This uncertainty is twofold, as it constitutes both an added value and a challenge for the forecaster and the user of the forecasts. Many authors have demonstrated the added (economic) value of probabilistic over deterministic forecasts across the water sector (e.g. flood protection, hydroelectric power management and navigation). However, the richness of the information is also a source of challenges for operational uses, due partially to the difficulty in transforming the probability of occurrence of an event into a binary decision. This paper presents the results of a risk-based decision-making game on the topic of flood protection mitigation, called "How much are you prepared to pay for a forecast?". The game was played at several workshops in 2015, which were attended by operational forecasters and academics working in the field of hydro-meteorology. The aim of this game was to better understand the role of probabilistic forecasts in decision-making processes and their perceived value by decision-makers. Based on the participants' willingness-to-pay for a forecast, the results of the game show that the value (or the usefulness) of a forecast depends on several factors, including the way users perceive the quality of their forecasts and link it to the perception of their own performances as decision-makers.

Ideally, semi-distributed hydrologic models should provide better streamflow simulations than lumped models, along with spatially-relevant water resources management solutions. However, the spatial distribution of model parameters raises issues related to the calibration strategy and to the identifiability of the parameters. To analyse these issues, we propose to base the evaluation of a semi-distributed model not only on its performance at streamflow gauging stations, but also on the spatial and temporal pattern of the optimised value of its parameters. We implemented calibration over 21 rolling periods and 64 catchments, and we analysed how well each parameter is identified in time and space. Performance and parameter identifiability are analysed comparatively to the calibration of the lumped version of the same model. We show that the semi-distributed model faces more difficulties to identify stable optimal parameter sets. The main difficulty lies in the identification of the parameters responsible for the closure of the water balance (i.e. for the particular model investigated, the intercatchment groundwater flow parameter).

A palladium mediated synthesis of a common synthon for the syntheses of antioxidant analogues of naturally occurring salvianolic acids is presented. The synthetic route may be used to obtain analogues with a balanced lipophilicity/hydrophilicity which may result in potentially interesting LDL antioxidants for the prevention of cardiovascular diseases.

The last decade has seen growing research in producing probabilistic hydro-meteorological forecasts and increasing their reliability. This followed the promise that, supplied with information about uncertainty, people would take better risk-based decisions. In recent years, therefore, research and operational developments have also started focusing attention on ways of communicating the probabilistic forecasts to decision-makers. Communicating probabilistic forecasts includes preparing tools and products for visualisation, but also requires understanding how decision-makers perceive and use uncertainty information in real time. At the EGU General Assembly 2012, we conducted a laboratory-style experiment in which several cases of flood forecasts and a choice of actions to take were presented as part of a game to participants, who acted as decision-makers. Answers were collected and analysed. In this paper, we present the results of this exercise and discuss if we indeed make better decisions on the basis of probabilistic forecasts.

Neuropeptide Y (NPY)/Agouti-related peptide (AgRP)-expressing neurons in the hypothalamus induce feeding and decrease energy expenditure. With consumption of a diet high in fat, there is an increase in circulating saturated free fatty acids, including palmitate, leading to the development of neuroinflammation and secretion of cytokines, such as TNFα, and in turn activation of the canonical IKKβ/NFκB cascade. We describe a model of palmitate- and TNFα-induced neuroinflammation in a functionally characterized, immortalized NPY/AgRP-expressing cell model, mHypoE-46, to study whether the anti-diabetic metformin alone or in combination with the anti-inflammatory agent salicylate can ameliorate these detrimental effects. Treatment with palmitate increased mRNA expression of feeding peptides Npy and Agrp, and inflammatory cytokines Tnfa and Il-6, whereas treatment with TNFα increased mRNA expression of Npy, Nfkb, Ikba, Tnfa, and Il-6. The effects of metformin and/or sodium salicylate on these genes were assessed. Metformin increased phosphorylation of AMPK and S6K, while sodium salicylate increased phospho-AMPK and decreased phospho-S6K, but neither had any effect on phospho-ERK, -JNK or -p38 in the mHypoE-46 NPY/AgRP neurons. Furthermore, we utilized a pre-treatment and/or co-treatment paradigm to model potential clinical regimens. We determined co-treatment with metformin or sodium salicylate alone was successful in alleviating changes observed in feeding peptide mRNA regulation, whereas a preventative pre-treatment with metformin and sodium salicylate together was able to alleviate palmitate- and TNFα-induced induction of NPY and/or AgRP mRNA levels. These results highlight important differences in reactive versus preventative treatments on palmitate- and TNFα-induced neuroinflammation in NPY/AgRP neurons.

The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when compared to the control group. Quantitative analysis demonstrated no significant differences between control and malnourished group by using Giemsa; however, as the organ reduction was not followed by an increase inversely proportional to the density of neurons, the condition imposed suggests the loss of neurons from the total population. Nevertheless, through NADPH-d histochemistry, there was a neuronal density increase for the malnourished group. There was no significant difference between the groups for both techniques with respect to the morphometric analysis of the body cell.Objetivou-se avaliar os efeitos da desnutrição protéica severa (4%) sobre os neurônios mientéricos do duodeno de ratos Wistar, comparando-se a uma dieta padrão para roedores de 22% de proteína. Para este estudo, segmentos do duodeno de 10 ratos de cada grupo nutricional foram submetidos à elaboração de preparados de membrana, sendo cinco corados pelo método de Giemsa, para estimar a população mientérica total e os demais corados pelo método histoquímico de evidenciação de células nervosas, por meio da atividade da enzima NADPH-diaforase para estudo da subpopulação de neurônios nitrérgicos. Aleatoriamente, foram mensuradas as áreas de 100 neurônios por animal, perfazendo um total de 2.000 neurônios, que foram mensurados através de um Sistema de Análise de Imagens Computadorizada - Image Pro-Plus®. Os ratos desnutridos apresentaram peso orporal 34,38% menor e uma redução no comprimento do duodeno de 10,60% quando comparado ao grupo controle. A análise quantitativa demonstrou não haver diferença significativa na comparação entre os grupos controle e desnutrido para técnica de Giemsa, porém como a redução do órgão não foi acompanhada por um aumento inversamente proporcional na densidade de neurônios, sugere-se que a condição imposta causou perda de neurônios na população total. Entretanto com a histoquímica da NADPH-d houve um umento da densidade neuronal no grupo desnutrido. Na análise morfométrica do perfil celular neuronal não houve diferença significativa entre os grupos em ambas as técnicas evidenciadas.

The aim of this paper is to investigate the use of statistical correction techniques in hydrological ensemble prediction. Ensemble weather forecasts (precipitation and temperature) are used as forcing variables to a hydrologic forecasting model for the production of ensemble streamflow forecasts. The impact of different bias correction strategies on the quality of the forecasts is examined. The performance of the system is evaluated when statistical processing is applied: to precipitation and temperature forecasts only (pre-processing from the hydrological model point of view), to flow forecasts (post-processing) and to both. The pre-processing technique combines precipitation ensemble predictions with an analog forecasting approach, while the post-processing is based on past errors of the hydrological model when simulating streamflows. Forecasts from 11 catchments in France are evaluated. Results illustrate the importance of taking into account hydrological uncertainties to improve the quality of operational streamflow forecasts.

In flow forecasting, additionally to the need of long time series of historic discharges for model setup and calibration, hydrological models also need real-time discharge data for the updating of the initial conditions at the time of the forecasts. The need of data challenges operational flow forecasting at ungauged or poorly gauged sites. This study evaluates the performance of different choices of parameter sets and discharge updates to run a flow forecasting model at ungauged sites, based on information from neighbour catchments. A cross-validation approach is applied on a set of 211 catchments in France and a 17-month forecasting period is used to calculate skill scores and evaluate the quality of the forecasts. A reference situation, where local information is available, is compared to alternative situations, which include scenarios where no local data is available at all and scenarios where local data started to be collected at the beginning of the forecasting period. To cope with uncertainties from rainfall forecasts, the model is driven by ensemble weather forecasts from the PEARP-Météo-France ensemble prediction system. The results show that neighbour catchments can contribute to provide forecasts of good quality at ungauged sites, especially with the transfer of parameter sets for model simulation. The added value of local data for the operational updating of the hydrological ensemble forecasts is highlighted.

An operational hydrological ensemble forecasting system based on a meteorological ensemble prediction system (M-EPS) coupled with a hydrological model searches to capture the uncertainties associated with the meteorological prediction to better predict river flows. However, the structure of the hydrological model is also an important source of uncertainty that has to be taken into account. This study aims at evaluating and comparing the performance and the reliability of different types of hydrological ensemble prediction systems (H-EPS), when ensemble weather forecasts are combined with a multi-model approach. The study is based on 29 catchments in France and 16 lumped hydrological model structures, driven by the weather forecasts from the European centre for medium-range weather forecasts (ECMWF). Results show that the ensemble predictions produced by a combination of several hydrological model structures and meteorological ensembles have higher skill and reliability than ensemble predictions given either by one single hydrological model fed by weather ensemble predictions or by several hydrological models and a deterministic meteorological forecast.

En este trabajo se prepararon dos carbones activados (CA), utilizando como agentes activantes ZnCl2 y K2CO3, denominados CA/ZnCl2, CA/K2CO3, respectivamente, a partir de residuos generados en el proceso de extracción del aceite esencial de candeia, árbol nativo de Brasil. Estos carbones se caracterizaron por microscopía electrónica de barrido, análisis termogravimétrico, análisis elemental, superficie específica por el método BET e índice de iodo. Estos materiales se probaron en la adsorción de dos moléculas modelos: el fenol y el azul de metileno, y se comparó su capacidad de adsorción con un carbón activado comercial (Merck), denominado CA/Com. Las isotermas de adsorción para CA/ZnCl2, CA/K2CO3 y CA/Com mostraron capacidades de adsorción máxima de 297, 228 y 271 mg g-1, para azul de metileno y de 109, 195 y 161 mg g-1, para el fenol, respectivamente. Los resultados evidenciaron que los carbones son eficientes en el proceso de adsorción de las moléculas modelo ensayadas. Las isotermas fueron ajustadas a las ecuaciones de Langmuir y de Freundlich. El modelo de Langmuir entregó un ajuste mejor, mostrando que los adsorbentes presentan superficies energéticamente más uniformes. Los carbones activados producidos se presentan como potenciales adsorbentes para compuestos orgánicos, agregando un valor adicional al residuo.

As all hydrological models are intrinsically limited hypotheses on the behaviour of catchments, models – which attempt to represent real-world behaviour – will always remain imperfect. To make progress on the long road towards improved models, we need demanding tests, i.e. true crash tests. Efficient testing requires large and varied data sets to develop and assess hydrological models, to ensure their generality, to diagnose their failures, and ultimately, help improving them.

This paper presents the development of the European Flood Alert System (EFAS), which aims at increasing preparedness for floods in trans-national European river basins by providing local water authorities with medium-range and probabilistic flood forecasting information 3 to 10 days in advance. The EFAS research project started in 2003 with the development of a prototype at the European Commission Joint Research Centre (JRC), in close collaboration with the national hydrological and meteorological services. The prototype covers the whole of Europe on a 5 km grid. In parallel, different high-resolution data sets have been collected for the Elbe and Danube river basins, allowing the potential of the system under optimum conditions and on a higher resolution to be assessed. Flood warning lead-times of 3–10 days are achieved through the incorporation of medium-range weather forecasts from the German Weather Service (DWD) and the European Centre for Medium-Range Weather Forecasts (ECMWF), comprising a full set of 51 probabilistic forecasts from the Ensemble Prediction System (EPS) provided by ECMWF. The ensemble of different hydrographs is analysed and combined to produce early flood warning information, which is disseminated to the hydrological services that have agreed to participate in the development of the system. In Part 1 of this paper, the scientific approach adopted in the development of the system is presented. The rational of the project, the system�s set-up, its underlying components, basic principles and products are described. In Part 2, results of a detailed statistical analysis of the performance of the system are shown, with regard to both probabilistic and deterministic forecasts.

Since 2005 the European Flood Alert System (EFAS) has been producing probabilistic hydrological forecasts in pre-operational mode at the Joint Research Centre (JRC) of the European Commission. EFAS aims at increasing preparedness for floods in trans-national European river basins by providing medium-range deterministic and probabilistic flood forecasting information, from 3 to 10 days in advance, to national hydro-meteorological services. This paper is Part 2 of a study presenting the development and skill assessment of EFAS. In Part 1, the scientific approach adopted in the development of the system has been presented, as well as its basic principles and forecast products. In the present article, two years of existing operational EFAS forecasts are statistically assessed and the skill of EFAS forecasts is analysed with several skill scores. The analysis is based on the comparison of threshold exceedances between proxy-observed and forecasted discharges. Skill is assessed both with and without taking into account the persistence of the forecasted signal during consecutive forecasts. Skill assessment approaches are mostly adopted from meteorology and the analysis also compares probabilistic and deterministic aspects of EFAS. Furthermore, the utility of different skill scores is discussed and their strengths and shortcomings illustrated. The analysis shows the benefit of incorporating past forecasts in the probability analysis, for medium-range forecasts, which effectively increases the skill of the forecasts.

The black, green and sour coffee defect (PVA) contributes with 20% of the total coffee production. It should be separate from the normal coffee grains in order to improve the final quality of the beverage. In this way, the present work has the objective to use the PVA reject for the production of activated carbon. The activated carbon (CA) was prepared from PVA defect using zinc chloride as activating agent. The prepared material (CA PVA) was characterized and the adsorption tests were carried out using as organic models methylene blue (AM) and reactive red (VR). The CA PVA revealed to be more efficient in the removal of the organic contaminants compared to a commercial activated carbon.

Este trabalho teve como objetivo avaliar o efeito do volume de calda aplicado por um turbopulverizador sobre a deposição e a cobertura em folhas, ramos e frutos de citros. A pulverização foi realizada com um pulverizador tratorizado Arbus 2000/Valência em pomar de laranja 'Natal', com porte médio de 4,0 m, sendo avaliados seis volumes de calda (50; 70; 85; 100; 150 e 200% do volume-padrão utilizado pelo produtor, de 28 L planta-1). Após a pulverização de plantas uniformes com calda contendo cobre e o traçador fluorescente Poliglow 830 YLSS, amostras foram coletadas em nove setores da planta, sendo a avaliação da deposição feita usando-se análise do íon cobre por espectrofotômetro de absorção atômica, e a da cobertura, por meio de imagens digitalizadas analisadas pelo programa para computador IDRISI. A análise estatística mostrou que, na avaliação da cobertura e deposição em citros, a utilização de frutos como estrutura de amostragem tendeu a evidenciar melhor o efeito dos tratamentos. Tanto a deposição quanto a cobertura tenderam a ser maiores nos setores frontal e saia da planta. Tanto a deposição quanto a cobertura não foram prejudicadas pela utilização do volume de 70% (19,6 L planta-1), indicando que tal volume pode substituir o volume de 100% (28 L planta-1) sem prejuízos ao controle de pragas.The aim of this study was to evaluate the efficacy of different carrier volume applied with an airblast sprayer on spray deposition on leaves, branch and fruits in citrus trees. It was used a trailed sprayer Arbus 200/Valência in an orange orchard, variety Natal, with 4,0 m mean height, and six volumes were evaluated (50; 70; 85; 100; 150 and 200% relative to the standard volume in use, 28 L plant-1). After spraying of regular plants with carrier containing copper and Poliglow 830 YLSS as fluorescent tracer, the samples were collected in nine localization of the tree. The evaluation of spray deposition was done with an atomic absorption spectrophotometer by analyze of copper ion. The evaluation of spray coverage was done by digitalized image which were analyzed with IDRISIS software. The analysis statistical showed that in the evaluation of spray coverage and deposition in citrus the use of fruits as sample made to evidence better the effect of the treatments. The spray coverage and deposition had been trend higher upon frontal horizontal sector and upon lower vertical sector of the tree. The spray coverage and deposition had not been prejudiced by the use of 70% rate (19.6 L plant-1) which indicates that this volume can replace the 100% rate (28.0 L plants-1) without disadvantage in the control periods.

Avaliou-se a influência da pressurização da calda de pulverização na viabilidade dos seguintes microrganismos entomopatogênicos: o fungo Metarhizium anisopliae, a bactéria Bacillus thuringiensis var. kurstaki, o nucleopoliedrovirus de Anticarsia gemmatalis (AgMNPV) e o nematóide Steinernema glaseri. Utilizou-se do sistema hidráulico de um pulverizador, sem filtros, equipado com ponta de pulverização de jato cônico JA-2. O delineamento experimental adotado foi o inteiramente casualizado, com sete tratamentos: pressurização da calda em 172; 345; 517; 689; 1.034 e 1.379 MPa, e a testemunha (calda não submetida à pressurização). A avaliação da viabilidade de M. anisopliae foi realizada por meio da porcentagem de germinação dos conídios. Para B. thuringiensis, foi considerado o número de colônias. A eficácia do AgMNPV foi estimada indiretamente, pela mortalidade causada às lagartas de A. gemmatalis no início do terceiro instar, alimentadas com dieta artificial impregnada com suspensão viral. No caso do S. glaseri, foi calculada a porcentagem de indivíduos vivos em lâmina de Peters. Conclui-se que se podem pulverizar os entomopatógenos M. anisopliae, B. thuringiensis, AgMNPV e S. glaseri, até a pressão de 1.379 MPa, sem que haja perda na viabilidade desses organismos utilizados como agentes de controle biológico.The objective of this research was to evaluate the influence of spraying pressurization upon the viability of the following entomopathogens microorganisms: the fungus Metarhizium anisopliae, the bacterium Bacillus thuringiensis var. kurstaki, the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV), and the nematode Steinernema glaseri. The sprayer hydraulical system was used without filters and equipped with JA-2 hollow-cone nozzle type. The experimental design was completely randomized, with seven treatments: pressurization of spraying suspension at 172; 345; 517; 689; 1,034 and 1,379 MPa, and check (suspension not submitted to the pressurization). Evaluation of the viability of M. anisopliae was estimated by the percentage of germinated conidia. For B. thuringiensis, by the number of colonies formed. Efficiency on AgMNPV was estimated indirectly, by means of mortality caused to A. gemmatalis infected as early third-instars larvae reared in an artificial diet impregnated with the viral suspension. For the experiment with S. glaseri it was calculated the percentage of live specimens on Peters slide-blade. It concluded that the entomopathogens M. anisopliae, B. thuringiensis, AgMNPV and S. glaseri can be sprayed up to the pressure of 1,379 MPa without losing the viability of those organisms as biological control agents.

No abstract available.

Diferentes tipos de pulverizadores são utilizados para a aplicação de herbicidas sendo que, em pequenas propriedades, é comum, por questões econômicas, a adaptação de barras ou pistolas manuais a tanques de grande capacidade. Por outro lado, em grandes propriedades, é crescente a tendência da substituição do sistema tradicional de reabastecimento dos pulverizadores pelo sistema de calda pronta. Em ambos os casos, pode haver a necessidade de um armazenamento prolongado nos tanques ou no veículo reabastecedor, principalmente na ocorrência de períodos prolongados de chuva. Torna-se, portanto, importante a determinação de períodos de tempo pelos quais as caldas de herbicidas possam ser armazenadas, sem que haja prejuízo à eficácia dos mesmos. O presente trabalho estudou os efeitos do tempo de armazenamento da calda sobre a eficácia de herbicidas aplicados em pré-emergência. O experimento foi instalado no delineamento experimental de blocos ao acaso, com três repetições, no ano agrícola 91/92, em área da Fazenda Experimental da Faculdade de Ciências Agrárias e Veterinárias da UNESP, município de Jaboticabal, Estado de São Paulo, Brasil. Avaliou-se as formulações comerciais de ametryne e diuron com 00, 05, 10, 15, 20, 25 e 30 dias de armazenamento da calda, além de uma testemunha, onde não se efetuou a aplicação de herbicidas. Foram realizadas contagens do número de emergências e altura, por espécie, das plantas daninhas em 1,2 m2 por parcela, aos 30, 45 e 59 dias após a aplicação e uma avaliação visual do controle geral por parcela aos 95 dias após a aplicação. Os resultados obtidos mostram que nenhum dos períodos de armazenamento testados interferiu significativamente na eficiência dos herbicidas, independente do produto utilizado (teste de F a 5%). Portanto, conclui-se que as caldas dos herbicidas testados puderam ser utilizadas normalmente, quando armazenadas por até 30 dias.Different types of sprayers are used in farm practice. In small farms it is getting more economical to couple spraying booms and manual spraying lances to large volume tanks. In large farms the trend is to replace the traditional system by the ready diluted mix system. In both cases a prolonged storing time of the mix in tanks or refilling vehicle, especially in rainy periods of the year is required. So, it is important to determine how long the storage time may be without causing the effectiveness of the diluted mix to decrease. That is what this paper is related to. A random block design trial was carried out during the 1991/92 crop season, at the Experimental Station of the Agronomy College of UNESP, in Jaboticabal, São Paulo, Brazil. Commercial diluted mixes of formulations of ametryne and diuron stored during 00, 05, 10, 15, 20, 25 and 30 days were field evaluated. A testimony plot was not treated. Countings of emerging weeds in a 1,2 m2 plots were perfomed at 30, 45 and 59 days after spraying. After a 95 day period a general visual control was also perfomed. Results showed that none of the storage times tested significantly influenced herbicides effectiveness, no matter what the product was. One concluded that the herbicides diluted mixes tested could be normally utilised, provided the storage time didn't exceed 30 days.

Diferentes tipos de pulverizadores são utilizados para a aplicação de herbicidas sendo que, em pequenas propriedades, é comum, por questões econômicas, a adaptação de barras ou pistolas manuais a tanques de grande capacidade. Por outro lado, em grandes propriedades, é crescente a tendência da substituição do sistema tradicional de reabastecimento dos pulverizadores pelo sistema de calda pronta. Em ambos os casos, pode haver a necessidade de um armazenamento prolongado nos tanques ou no veículo reabastecedor, principalmente na ocorrência de períodos prolongados de chuva. Torna-se, portanto, importante a determinação de períodos de tempo pelos quais as caldas de herbicidas possam ser armazenadas, sem que haja prejuízo à eficácia dos mesmos. O presente trabalho estudou os efeitos do tempo de armazenamento da calda sobre a eficácia de herbicidas aplicados em pós-emergência. O experimento foi instalado no delineamento experimental de blocos ao acaso, com três repetições, no ano agrícola de 91/92, em Área da Fazenda Experimental da Faculdade de Ciências Agrárias e Veterinárias da UNESP, município de Jaboticabal, Estado de São Paulo, Brasil. Avaliou-se as formulações comerciais de glyphosate, mistura pronta de glyphosate + 2,4?D, MSMA e paraquat com 00, 05, 10, 15, 20, 25 e 30 dias de armazenamento da calda, além de uma testemunha, onde não se efetuou a aplicação de herbicidas. Foram realizadas avaliações visuais de controle, geral e por espécie, aos 15, 31 e 46 dias após a aplicação e os resultados obtidos mostram que nenhum dos períodos de armazenamento testados interferiu significativamente na eficiência dos herbicidas (teste de F a 5%), independente do produto utilizado e da época de avaliação. Portanto, conclui-se que as caldas dos herbicidas testados puderam ser utilizadas normalmente, quando armazenadas por até 30 dias.Different types of sprayers are used in farm practice. In small farms it is getting more economical to couple spraying booms and manual spraying lances to large volume tanks. In large farms the trend is to replace the traditional system by the ready diluted mix system. In both cases a prolonged storing time of the mix in tanks or refilling vehicle, especially in rainy periods of the year is required. So, it is important to determine how long the storage time may be without causing the effectiveness of the diluted mix to decrease. That is what this paper is related to. A random block design trial was carried out during the 1991/92 crop season, at the Experimental Station of the Agronomy College of UNESP, in Jaboticabal, São Paulo, Brazil. Diluted mixes of commercial formulation of glyphosate, MSMA and paraquat beyond a ready-for-use glyphosate + 2,4-D amine mix were evaluted at 00, 05, 10, 15, 20, 25 and 30 days of storage. A testimony plot was not spraied. Visual control evaluations, both general and by species were perfomed at 15, 31 and 46 days after spraying. The results showed no influence of storage periods on herbicides efficiencies (Tukey, 5%) no matter independent of the commercial product and time of evaluation. One concluded that the diluted mixes can be normally used provided the storage time doesn't exceed 30 days.

Um dos herbicidas mais utilizados para o controle de plantas daninhas em pós-emergência nos pomares cítricos paulistas é o glyphosate. No entanto, este herbicida aplicado isoladamente e nas doses recomendadas, tem proporcionado seleção da planta daninha trapoeraba (Commelina virginica L.), devido à grande tolerância da mesma, somada à eficiente eliminação das demais espécies da comunidade infestante. Em vista disso, e pela falta de opções , faz-se necessária a pesquisa de outros herbicidas ou misturas de herbicidas que sejam eficientes no controle da trapoeraba, de baixa toxicidade para os aplicadores, sistêmicos, e que sejam seletivos às plantas cítricas. O presente trabalho foi desenvolvido com o objetivo de estudar a eficiência da mistura pronta dos herbicidas glyphosate + 2,4-D amina no controle da trapoeraba em citros [Citrus sinensis (L.) Osbeck], em comparação com estes mesmos herbicidas aplicados isoladamente, bem como os possíveis efeitos fitotóxicos da mesma à cultura. O experimento foi instalado na região de Catanduva-SP, em um pomar de laranja Pera clone Rio, enxertada sobre limão Cravo, com dez anos de idade, plantado em um espaçamento de 8,0 x 6,5 m. A aplicação dos herbicidas foi realizada em 20 de fevereiro de 1991, de forma dirigida, em pós-emergência tardia da trapoeraba, quando a mesma já florescia e tinha altura variável entre 15 e 60 cm. A análise e interpretação dos resultados obtidos mostraram que a mistura pronta de glyphosate + 2,4-D apresenta um controle superior da trapoeraba em relação aos produtos aplicados isoladamente, não havendo diferenças significativas no controle para doses superiores a 0,60 + 0,80 kg i.a./ha. Os dados obtidos reforçam a teoria de sinergismo entre os dois produtos. Em nenhum dos tratamentos foi verificado sintomas visuais de intoxicação nas laranjeiras.In citrus orchard of São Paulo state, Brazil, one of the most employed post-emergency herbicide for weed control is glyphosate. However, when this herbicide is applied alone and at the recommended rates, it has been observed a selection of the weed Commelina virginica L. due to its great tolerance and the elimination of other weeds. Because of that, other chemicals must be studied to solve the problem. In this particular case, the herbicide should be sistemic and have a low toxicity to the operator and to the citrus trees. This work was conducted to study the efficacy of a ready mixture of glyphosate + 2,4-D compared to the products sprayed alone in orange orchards [Citrus sinensis (L.) Osbeck]. The phytotoxicity effects were studied too. The experiment was carried out in Catanduva, São Paulo, Brazil, in a ten years old orange orchard, with 8,0 x 6,0m spacing. On february, 20 th, 1991, the herbicides were sprayed directly on 15-60cm height Commelina during its flowering. The results showed that the ready mixture was better in Commelina control than other treatments. No significant differences were observed for the rates greater than 0,60 + 0,80 kg a.i./ha. The results reinforced the sinergism theory within both products. None of the treatments caused visual phytotoxicity to orange trees.

Squamous cell lung carcinomas account for approximately 25% of new lung carcinoma cases and 40,000 deaths per year in the United States. Although there are multiple genomically targeted therapies for lung adenocarcinoma, none has yet been reported in squamous cell lung carcinoma.Using SNP array analysis, we found that a region of chromosome segment 8p11-12 containing three genes-WHSC1L1, LETM2, and FGFR1-is amplified in 3% of lung adenocarcinomas and 21% of squamous cell lung carcinomas. Furthermore, we demonstrated that a non-small cell lung carcinoma cell line harboring focal amplification of FGFR1 is dependent on FGFR1 activity for cell growth, as treatment of this cell line either with FGFR1-specific shRNAs or with FGFR small molecule enzymatic inhibitors leads to cell growth inhibition.These studies show that FGFR1 amplification is common in squamous cell lung cancer, and that FGFR1 may represent a promising therapeutic target in non-small cell lung cancer.

Objective: To compare the therapeutic efficacy of kalachuchi (Plumeria acuminata Ait.) bark extract ointment (KO) and clotrimazole cream 1% (CC) in the treatment of otomycosis. Methods: Design: Randomized double blind controlled trial Setting: Outpatient otorhinolaryngology clinic of a tertiary private hospital Subjects: Patients aged 18-years-old and above diagnosed clinically to have otomycosis with a positive potassium hydroxide (KOH) smear were randomly assigned to kalachuchi (Plumeria acuminata Ait.) bark extract ointment or clotrimazole cream 1% in unlabeled containers. Self-application thrice daily for two weeks followed initial detailed instructions and demonstration. Symptoms, physical findings and repeat KOH smears were recorded after the first and second weeks of treatment. Results: Eighteen patients with otomycosis were enrolled in the study. There was one dropout per treatment group with no intention to treat. There were no statistically significant differences between KO and CC, with 75% (n=8) and 87.5% (n=8) cure rates, respectively. One subject in the KO arm reported severe ear pain. Conclusion: Kalachuchi extract ointment may be a promising topical antifungal agent. Multicenter clinical trials to establish its efficacy and safety as an effective alternative in the management of otomycosis should be conducted. Key words: Otomycosis, kalachuchi, Plumeria acuminata Ait., clotrimazole, clinical trial, herbal medicine

Objective: To present our experience with the anterolateral thigh flap in reconstructing a full thickness defect of the buccal mucosa and cheek. Methods: Design: Case Report Setting: Tertiary Private Hospital Patient: One Results: A 36 year old male with a T4aN0M0 Stage IVa buccal carcinoma on the left underwent wide excision, marginal mandibulectomy and modified radical neck dissection with preservation of the spinal accessory nerve, internal jugular vein and sternocleidomastoid muscle. The resultant through-and-through defect of the cheek skin measuring 8 x 6cm and buccal mucosa measuring 6 x 10 cm with a concomitant ¼ upper lip and 1/3 lower lip defect was reconstructed with an anterolateral thigh free flap. The patient recovered uneventfully and underwent adjuvant concurrent chemoradiotherapy 1 month post-operation. At 2 ½ months post-operation, he had no oral incontinence and could resume a normal diet with good speech. Conclusion: The anterolateral thigh free flap is an excellent soft-tissue flap for reconstruction of a full thickness defect of the buccal area. Key words: Surgical flap, Carcinoma, squamous cell, Oral cancer