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Your search keyword '"Griffith, Linda G."' showing total 334 results
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334 results on '"Griffith, Linda G."'

2. A Call for New Theories on the Pathogenesis and Pathophysiology of Endometriosis

Author

Canis, Michel, Abbott, Jason, Abrao, Mauricio, Al-Jefout, Moamar, Bedaiwy, Mohamed, Benagiano, Giuseppe, Burns, Katherine A., Carmona, Francisco, Chapron, Charles, Critchley, Hilary O.D., de Ziegler, Dominique, Falcone, Tommaso, Fazleabas, Asgerally, Ferrero, Simone, Garcia-Velasco, Juan A., Gargett, Caroline, Greaves, Erin, Griffith, Linda G., Guo, Sun-Wei, Habiba, Marwan, Harada, Tasuku, Hull, Mary Louise, Johnson, Neil P., Kaufman, Yuval, Issacson, Keith B, Khan, Khaleque N., Kim, Mee-Ran, Koninckx, Philippe A., Lessey, Bruce A., Martin, Dan, Matsuzaki, Sachiko, Mishra, Gita, Osuga, Yutaka, Petraglia, Felice, Popov, Alexander, Roman, Horace, Romano, Andrea, Saunders, Philippa, Seckin, Tamer, Somigliana, Edgardo, Taylor, Hugh S., Taylor, Robert N., Vercellini, Paolo, and Vigano, Paola

Published
2024
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4. A microfluidic hanging droplet as a programmable platform for mammalian egg vitrification.

Author

Feng, Haidong, Katsikis, Georgios, Napier, India D., Du, Gong, Lim, Josh, Doyle, Joseph O., Manalis, Scott R., and Griffith, Linda G.

Subjects
CHILDBEARING age, IMMERSION in liquids, CLINICAL medicine research, LIQUID nitrogen, REPRODUCTIVE health, EMBRYOLOGY
Abstract

Egg (oocyte) vitrification is the dominant method for preserving fertility for women of reproductive age. However, the method is typically performed by hand, requiring precise (∼0.1 to 10 μL) and time-sensitive (∼1 s) liquid exchange of cryoprotectants (CPA) around eggs as well as fine handling of eggs (∼100 μm) for immersion into liquid nitrogen (LN2). Here, we developed a microfluidic platform for programmable vitrification. Our platform is based on a millimeter-sized hanging droplet inside which a given egg is suspended and subjected to liquid exchanges within seconds. After programmable exposures to CPA, the egg is extracted from the liquid–air interface of the droplet using a motorized fine-tip instrument and immersed into LN2 for vitrification. To benchmark our platform with the manual method, we vitrified over a hundred mouse eggs and found comparable percentages (∼95%) for post-vitrification survivability. In addition, our platform performs real-time microscopy of the egg thereby enabling future studies where its morphology may be linked to functional outcomes. Our study contributes to the ongoing efforts to enhance the automation of embryology techniques towards broader applications in reproductive medicine both for clinical and research purposes. [ABSTRACT FROM AUTHOR]

Published
2024
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6. A microenvironment-inspired synthetic three-dimensional model for pancreatic ductal adenocarcinoma organoids

Author

Below, Christopher R., Kelly, Joanna, Brown, Alexander, Humphries, Jonathan D., Hutton, Colin, Xu, Jingshu, Lee, Brian Y., Cintas, Celia, Zhang, Xiaohong, Hernandez-Gordillo, Victor, Stockdale, Linda, Goldsworthy, Matthew A., Geraghty, Joe, Foster, Lucy, O’Reilly, Derek A., Schedding, Barbara, Askari, Janet, Burns, Jessica, Hodson, Nigel, Smith, Duncan L., Lally, Catherine, Ashton, Garry, Knight, David, Mironov, Aleksandr, Banyard, Antonia, Eble, Johannes A., Morton, Jennifer P., Humphries, Martin J., Griffith, Linda G., and Jørgensen, Claus

Published
2022
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7. The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance

Author

Dall’Agnese, Alessandra, Platt, Jesse M., Zheng, Ming M., Friesen, Max, Dall’Agnese, Giuseppe, Blaise, Alyssa M., Spinelli, Jessica B., Henninger, Jonathan E., Tevonian, Erin N., Hannett, Nancy M., Lazaris, Charalampos, Drescher, Hannah K., Bartsch, Lea M., Kilgore, Henry R., Jaenisch, Rudolf, Griffith, Linda G., Cisse, Ibrahim I., Jeppesen, Jacob F., Lee, Tong I., and Young, Richard A.

Published
2022
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10. Primary Human Colonic Mucosal Barrier Crosstalk with Super Oxygen-Sensitive Faecalibacterium prausnitzii in Continuous Culture

Author

Zhang, Jianbo, Huang, Yu-Ja, Yoon, Jun Young, Kemmitt, John, Wright, Charles, Schneider, Kirsten, Sphabmixay, Pierre, Hernandez-Gordillo, Victor, Holcomb, Steven J., Bhushan, Brij, Rohatgi, Gar, Benton, Kyle, Carpenter, David, Kester, Jemila C., Eng, George, Breault, David T., Yilmaz, Omer, Taketani, Mao, Voigt, Christopher A., Carrier, Rebecca L., Trumper, David L., and Griffith, Linda G.

Published
2021
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19. Report of the Assay Guidance Workshop on 3-Dimensional Tissue Models for Antiviral Drug Development.

Author

Jordan, Robert, Ford-Scheimer, Stephanie L, Alarcon, Rodolfo M, Atala, Anthony, Borenstein, Jeffrey T, Brimacombe, Kyle R, Cherry, Sara, Clevers, Hans, Davis, Mindy I, Funnell, Simon G P, Gehrke, Lee, Griffith, Linda G, Grossman, Abigail C, Hartung, Thomas, Ingber, Donald E, Kleinstreuer, Nicole C, Kuo, Calvin J, Lee, Emily M, Mummery, Christine L, and Pickett, Thames E

Subjects
ANTIVIRAL agents, DRUG development, DRUG discovery, PLURIPOTENT stem cells, TISSUES
Abstract

The National Center for Advancing Translational Sciences (NCATS) Assay Guidance Manual (AGM) Workshop on 3D Tissue Models for Antiviral Drug Development, held virtually on 7–8 June 2022, provided comprehensive coverage of critical concepts intended to help scientists establish robust, reproducible, and scalable 3D tissue models to study viruses with pandemic potential. This workshop was organized by NCATS, the National Institute of Allergy and Infectious Diseases, and the Bill and Melinda Gates Foundation. During the workshop, scientific experts from academia, industry, and government provided an overview of 3D tissue models' utility and limitations, use of existing 3D tissue models for antiviral drug development, practical advice, best practices, and case studies about the application of available 3D tissue models to infectious disease modeling. This report includes a summary of each workshop session as well as a discussion of perspectives and challenges related to the use of 3D tissues in antiviral drug discovery. [ABSTRACT FROM AUTHOR]

Published
2023
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21. “I’m looking through you”: What consumers and manufacturers need to know about non-invasive diagnostic tests for endometriosis

Author

Vigano’, Paola, Vercellini, Paolo, Somigliana, Edgardo, Chapron, Charles, Petraglia, Felice, Griffith, Linda G., Mueller, Michael D., Martin, Dan C., Nap, Annemiek, Romano, Andrea, Reis, Fernando, Carmona, Francisco, Burns, Katherine A., Taylor, Hugh S., Santulli, Pietro, Canis, Michel, Koninckx, Philippe R., and Guo, Sun-Wei

Published
2023
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34. The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance.

Author

Dall'Agnese, Alessandra, Platt, Jesse M., Zheng, Ming M., Friesen, Max, Dall'Agnese, Giuseppe, Blaise, Alyssa M., Spinelli, Jessica B., Henninger, Jonathan E., Tevonian, Erin N., Hannett, Nancy M., Lazaris, Charalampos, Drescher, Hannah K., Bartsch, Lea M., Kilgore, Henry R., Jaenisch, Rudolf, Griffith, Linda G., Cisse, Ibrahim I., Jeppesen, Jacob F., Lee, Tong I., and Young, Richard A.

Subjects
INSULIN receptors, INSULIN resistance, TYPE 2 diabetes, THERAPEUTICS, CELL membranes, ADIPOGENESIS
Abstract

Insulin receptor (IR) signaling is central to normal metabolic control and is dysregulated in metabolic diseases such as type 2 diabetes. We report here that IR is incorporated into dynamic clusters at the plasma membrane, in the cytoplasm and in the nucleus of human hepatocytes and adipocytes. Insulin stimulation promotes further incorporation of IR into these dynamic clusters in insulin-sensitive cells but not in insulin-resistant cells, where both IR accumulation and dynamic behavior are reduced. Treatment of insulin-resistant cells with metformin, a first-line drug used to treat type 2 diabetes, can rescue IR accumulation and the dynamic behavior of these clusters. This rescue is associated with metformin's role in reducing reactive oxygen species that interfere with normal dynamics. These results indicate that changes in the physico-mechanical features of IR clusters contribute to insulin resistance and have implications for improved therapeutic approaches. The authors find that the insulin receptor forms dynamic clusters during insulin signaling and that these clusters become dysfunctional in insulin resistance. This dysfunction is partially rescued by metformin, a drug used to treat type 2 diabetes. [ABSTRACT FROM AUTHOR]

Published
2022
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38. Biology-inspired microphysiological systems to advance patient benefit and animal welfare in drug development

Author

Marx, Uwe, Akabane, Takafumi, Andersson, Tommy B., Baker, Elizabeth, Beilmann, Mario, Beken, Sonja, Brendler-Schwaab, Susanne, Cirit, Murat, David, Rhiannon, Dehne, Eva-Maria, Durieux, Isabell, Ewart, Lorna, Fitzpatrick, Suzanne C., Frey, Olivier, Fuchs, Florian, Griffith, Linda G., Hamilton, Geraldine A., Hartung, Thomas, Hoeng, Julia, Hogberg, Helena, Hughes, David J., Ingber, Donald E., Iskandar, Anita, Kanamori, Toshiyuki, Kojima, Hajime, Kuehnl, Jochen, Leist, Marcel, Li, Bo, Loskill, Peter, Mendrick, Donna L., Neumann, Thomas, Pallocca, Giorgia, Rusyn, Ivan, Smirnova, Lena, Steger-Hartmann, Thomas, Tagle, Danilo A., Tonevitsky, Alexander, Tsyb, Sergej, Trapecar, Martin, van de Water, Bob, van den Eijnden-van Raaij, Janny, Vulto, Paul, Watanabe, Kengo, Wolf, Armin, Zhou, Xiaobing, Roth, Adrian, and Publica

Subjects
Drug Development, Drug Industry, Lab-On-A-Chip Devices, ddc:570, Drug Evaluation, Preclinical, Animals, Humans, Animal Testing Alternatives, Animal Welfare, Models, Biological, Article
Abstract

The first microfluidic microphysiological systems (MPS) entered the academic scene more than 15 years ago and were considered an enabling technology to human (patho)biology in vitro and, therefore, provide alternative approaches to laboratory animals in pharmaceutical drug development and academic research. Nowadays, the field generates more than a thousand scientific publications per year. Despite the MPS hype in academia and by platform providers, which says this technology is about to reshape the entire in vitro culture landscape in basic and applied research, MPS approaches have neither been widely adopted by the pharmaceutical industry yet nor reached regulated drug authorization processes at all.Here, 46 leading experts from all stakeholders - academia, MPS supplier industry, pharmaceutical and consumer products industries, and leading regulatory agencies - worldwide have analyzed existing challenges and hurdles along the MPS-based assay life cycle in a second workshop of this kind in June 2019. They identified that the level of qualification of MPS-based assays for a given context of use and a communication gap between stakeholders are the major challenges for industrial adoption by end-users. Finally, a regulatory acceptance dilemma exists against that background. This t4 report elaborates on these findings in detail and summarizes solutions how to overcome the roadblocks. It provides recommendations and a roadmap towards regulatory accepted MPS-based models and assays for patients' benefit and further laboratory animal reduction in drug development. Finally, experts highlighted the potential of MPS-based human disease models to feedback into laboratory animal replacement in basic life science research. published

Published
2020

40. Microfluidic shear devices for quantitative analysis of cell adhesion

Author

Lu, Hang, Koo, Lily Y., Wang, Wenchung M., Lauffenburger, Douglas A., Griffith, Linda G., and Jensen, Klavs F.

Subjects
Chemistry, Analytic -- Research, Cell adhesion -- Research, Chemistry
Abstract

We describe the design, construction, and characterization of microfluidic devices for studying cell adhesion and cell mechanics. The method offers multiple advantages over previous approaches, including a wide range of distractive forces, high-throughput performance, simplicity in experimental setup and control, and potential for integration with other microanalytic modules. By manipulating the geometry and surface chemistry of the microdevices, we are able to vary the shear force and the biochemistry during an experiment. The dynamics of cell detachment under different conditions can be captured simultaneously using time-lapse videomicroscopy. We demonstrate assessment of cell adhesion to fibronectin-coated substrates as a function of the shear stress or fibronectin concentration in microchannels. Furthermore, a combined perfusion-shear device is designed to maintain cell viability for long-term culture as well as to introduce exogenous reagents for biochemical studies of cell adhesion regulation. In agreement with established literature, we show that fibroblasts cultured in the combined device reduced their adhesion strength to the substrate in response to epidermal growth factor stimulation.

Published
2004

45. Flux-Biased, Energy-Efficient Electromagnetic Micropumps Utilizing Bistable Magnetic Latching and Energy-Storage Springs.

Author

Bhushan, Brij M., Yoon, Jun Young, Griffith, Linda G., and Trumper, David L.

Abstract

On-platform pumping systems are a potentially critical technology for microphysiological systems (MPS) to control the pressure and flow of growth media. Supporting sufficient physiological tissue quantity in culture requires fluid flow rates on the order of microliters per second, which is larger than for typical microfluidic systems. Thus, a need exists for new types of pumping systems operating in this flow range while maintaining stringent sterility of the culture as well as enabling tight temperature control at ${37}^{\circ } {\rm C}$. Flow rates and pressure also need to be readily computer-controlled, with a manageable set of connections into the culture incubator environment. This article describes a novel mesoscale electromagnetically driven pumping system designed to meet all these requirements. Our design achieves a very low energy dissipation of ${0.65}\,{\rm mJ}$ per switching event, which allows one pumping channel to operate at ${0.45}\,{{\rm {\mu } L} /{\rm s}}$ with an average power dissipation of 1.3 mW and ${0.04}\,{{^\circ } {\rm C}}$ temperature rise in each actuator. The actuator operates in a bistable, teeter-totter configuration with a latching force of ${4.5}\,{\rm N}$ , and a relatively large stroke of ${400}\,{{\mu } {\rm m}}$ at the actuator pole face. Preliminary operational pumping test results show the potential of this type of electromagnetic actuator for fluidic pumping. Due to their compact configuration and very high energy efficiency, these pumps can provide the foundation for multichannel, on-platform pumping for MPS platforms, as well as for a range of sterile, temperature-sensitive microflow devices such as portable, battery-operated insulin pumps. [ABSTRACT FROM AUTHOR]

Published
2021
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50. IP-10 (CXCL10) Can Trigger Emergence of Dormant Breast Cancer Cells in a Metastatic Liver Microenvironment.

Author

Clark, Amanda M., Heusey, Haley L., Griffith, Linda G., Lauffenburger, Douglas. A., and Wells, Alan

Subjects
METASTATIC breast cancer, LIVER cells, BREAST cancer, PROGNOSIS, INCURABLE diseases, CANCER cell growth
Abstract

Metastatic breast cancer remains a largely incurable and fatal disease with liver involvement bearing the worst prognosis. The danger is compounded by a subset of disseminated tumor cells that may lie dormant for years to decades before re-emerging as clinically detectable metastases. Pathophysiological signals can drive these tumor cells to emerge. Prior studies indicated CXCR3 ligands as being the predominant signals synergistically and significantly unregulated during inflammation in the gut-liver axis. Of the CXCR3 ligands, IP-10 (CXCL10) was the most abundant, correlated significantly with shortened survival of human breast cancer patients with metastatic disease and was highest in those with triple negative (TNBC) disease. Using a complex ex vivo all-human liver microphysiological (MPS) model of dormant-emergent metastatic progression, CXCR3 ligands were found to be elevated in actively growing populations of metastatic TNBC breast cancer cells whereas they remained similar to the tumor-free hepatic niche in those with dormant breast cancer cells. Subsequent stimulation of dormant breast cancer cells in the ex vivo metastatic liver MPS model with IP-10 triggered their emergence in a dose-dependent manner. Emergence was indicated to occur indirectly possibly via activation of the resident liver cells in the surrounding metastatic microenvironment, as stimulation of breast cancer cells with exogenous IP-10 did not significantly change their migratory, invasive or proliferative behavior. The findings reveal that IP-10 is capable of triggering the emergence of dormant breast cancer cells within the liver metastatic niche and identifies the IP-10/CXCR3 as a candidate targetable pathway for rational approaches aimed at maintaining dormancy. [ABSTRACT FROM AUTHOR]

Published
2021
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