1. Performance of BinaxNOW G6PD Deficiency Point-of-Care Diagnostic in P. vivax-Infected Subjects
- Author
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Sowmya Gopalan, Justin A. Green, Dhanpat K. Kochar, Ramadurai Srinivasan, Nick Carter, Harald Noedl, Sandeep K. Gupta, Ronnatrai Rueangweerayut, Marcus V. G. Lacerda, Moritz Treiber, Jörg J. Möhrle, Lyda Osorio, Sanjay K. Kochar, Alejandro Llanos-Cuentas, Preetam Arthur, Srivicha Krudsood, and Germana Bancone
- Subjects
medicine.medical_specialty ,G6PD activity ,Point-of-Care Systems ,Glycogen Storage Disease Type I ,Sensitivity and Specificity ,Antimalarials ,Virology ,Internal medicine ,parasitic diseases ,medicine ,Malaria, Vivax ,Humans ,Malaria, Vivax/drug therapy ,G hemoglobin ,Point of care ,business.industry ,Diagnostic test ,Articles ,medicine.disease ,Hemolysis ,Surgery ,Highly sensitive ,Infectious Diseases ,Glycogen Storage Disease Type I/diagnosis ,Quantitative assay ,Antimalarials/therapeutic use ,Parasitology ,business ,purl.org/pe-repo/ocde/ford#3.03.06 [https] ,Malaria - Abstract
Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 Plasmodium vivax-infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05-20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18-25 degrees C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas.
- Published
- 2015