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In non-climacteric fruit tree species, color change is primarily controlled by the interplay between environmental conditions, nutritional factors (nitrogen and sugars) and hormones, mainly abscisic acid and gibberellins (GA), through a complex mechanism which is not completely understood. Nitrogen has a strong impact on color change, influenced by environmental changes, either locally or at the whole tree level. We use Citrus trees, as a non-climacteric model species, to understand the long-distance nitrogen signaling mechanism delaying the chloroplast-to-chromoplast transition at the molecular level. It is unknown whether nitrogen regulates the branch point of geranylgeranyl diphosphate (GGPP), the shared precursor for gibberellin, chlorophyll and carotenoid synthesis. We used 15N to trace the root-to-fruit nitrogen flux in trees grown under soils differing in nitrogen content and temperature, and treated with NH4NO3 and GA. Key genes involved in nitrogen signaling, transport and metabolism, and those from the GGPP branch point were analyzed in the fruit. Results explain how soil temperature modifies 15N transport to play a key role in signaling citrus color change, and show that fruit-nitrogen remobilization is required for triggering degreening. Nitrogen content in the fruit modulates the onset of glutamate deamination, asparagine synthesis, nitrite assimilation and GA1 depletion. Expression of the nitrate transporter NRT1.2, glutamate dehydrogenase and asparagine synthetase genes was high right before the start of degreening, together with NH4+ concentration. Nitrogen delayed carotenoid synthesis (phytoene synthase gene expression) without modifying gibberellin synthesis (ent-kaurene synthase and oxidase) at the chloroplast level., This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

The SPIDER Beam Source (BS), the first prototype of a full scale ion source for the ITER Heating Neutral Beam injector, was delivered to the Neutral Beam Test Facility (NBTF) site in Padova (Italy) after about five years procurement phase. A huge effort was devoted during the procurement for quality controls and testing at the supplier’s workshops. Several activities were also carried out on NBTF site for verification/adjustment of interfaces, solution of still open issues, as well as final tests before and after installation inside the vacuum vessel. The NBTF Team undertook the BS site acceptance tests including: pressure and leak tests of the hydraulic circuits; electrical tests; measurement of magnetic field profiles; functionality tests of diagnostics installed on the BS; checks of grids alignment by means of laser tracker. Accurate positioning of the BS inside the vacuum vessel was performed and various service lines were connected in a tight space. Several improvements were undertaken in order to guarantee reliability and reduce the risks during the commissioning and experimental campaign in 2018. After installation, the integrated commissioning phase was initiated, powering the RF and high voltage circuits, followed by the first operation in vacuum.

A set of novel design solutions for high performance cooling systems have been developed and tested by Consorzio RFX, achieving, with experimental tests, the challenging heat transfer conditions foreseen for Heating and Current Drive Systems of present and future nuclear fusion devices. The project, called Multi-design Innovative Cooling Research & Optimization (MICRO), has the triple objective of: • Verifying the full qualification of the manufacturing process and assess the cooling performance of the present solutions applied on the acceleration grids designed for ITER Heating Neutral Beam Injectors and MITICA experiment, which have similar heat loads as for DEMO1. • Identifying alternative designs realizable with presently qualified manufacturing processes for improving heat transfer mechanisms with limited pressure drops. • Developing further optimized solutions by means of the investigation of the interrelated effects of geometric parameters and thermofluid dynamic properties of the coolant fluid. The main aim of present work is to extend the fatigue life-cycle of high thermal stress components. In the first part of the paper the design description and constraints are presented together with the results obtained from numerical analyses carried out on the full-scaled model of the electrostatic grids; the outcomes of the experimental campaign, together with the characterization of the properties and performance of a set of alternative dielectric fluids, are shown in the second one.

In June 2018 the SPIDER device, which is the full-size prototype of the negative ion source and extractor for the ITER Heating Neutral Beam has entered the first operation phase at the Neutral Beam Test Facility (NBTF) in Padova, Italy. This paper describes the present status of the device, the experimental plans and the results obtained during the first experimental campaign of the radio-frequency driven plasma source.

The realization of the ITER Neutral Beam Test Facility (NBTF) and the start the experimental phase are important tasks of the fusion roadmap, since the target requirements of injecting to the plasma a beam of Deuterium atoms with a power up to 16.5 MW, at 1MeV of energy and with a pulse length up to 3600s have never been reached together before. The ITER NBTF, called PRIMA (Padova Research on ITER Megavolt Accelerator), is hosted in Padova, Italy; it includes two experiments: MITICA, the full-scale prototype of the ITER injector and SPIDER, the full-size negative ion source. The realization promoted by the ITER organization is carried out with the contribution of the European Union, channeled through the Joint Undertaking for ITER (F4E), of the Consorzio RFX which hosts the Test Facility, the Japanese and the Indian ITER Domestic Agencies (JADA and INDA) and several European laboratories, such as IPP-Garching, KIT-Karlsruhe, CCFECulham, CEA-Cadarache. The early start of operation of PRIMA experiments is urgent because sufficient experimental time is necessary to face and solve the issues related to the achievement of the desired performance in time for the ITER operation, requiring NBI since the beginning. Substantial progresses have been recently achieved: the buildings construction, begun in October 2012, has been completed by the end of 2015 and the installation of some components has been started since the end of 2014. The SPIDER realization is well advanced: the installation phase is proceeding in good agreement with the general plan; it is expected to be almost completed by the end of 2016. In parallel, the commissioning of the SPIDER power supply (PS) and auxiliary plant systems is being proceeding. Tests at full power and remote control are planned, including also those addressed to reproduce the grid breakdowns and to test the relevant protections. The design of the MITICA injector components was completed in 2015their procurement is being made through a number of tenders, some of them already launched. The HV Power Supply system of the MITICA 1MV accelerator, provided by JADA, was delivered on site in December 2015. The challenging installation of these components, including the step-up transformers and the SF6 gas insulated transmission line, started soon after and will go on throughout 2016. The present phase, with the PRIMA buildings continuously filled with new components, with the installation activities progressing and with also the commissioning and testing phase starting represents a substantial step forward toward the main target. The paper will describe the main challenges the Project Team has dealt during this phase and the important feedback derived for the ITER HNB systems both from the technical and the organizational standpoints.

SPIDER is one of two projects of the ITER Neutral Beam Test Facility under construction in Padova, Italy, at the Consorzio RFX premises. It will have a 100 keV beam source with a full-size prototype of the radiofrequency ion source for the ITER neutral beam injector (NBI) and also, similar to the ITER diagnostic neutral beam, it is designed to operate with a pulse length of up to 3600 s, featuring an ITER-like magnetic filter field configuration (for high extraction of negative ions) and caesium oven (for high production of negative ions) layout as well as a wide set of diagnostics. These features will allow a reproduction of the ion source operation in ITER, which cannot be done in any other existing test facility. SPIDER realization is well advanced and the first operation is expected at the beginning of 2018, with the mission of achieving the ITER heating and diagnostic NBI ion source requirements and of improving its performance in terms of reliability and availability. This paper mainly focuses on the preparation of the first SPIDER operations - integration and testing of SPIDER components, completion and implementation of diagnostics and control and formulation of operation and research plan, based on a staged strategy

SPIDER is one of the two projects of the ITER Neutral Beam Test Facility (NBTF) under construction in Padova, Italy, at Consorzio RFX premises; it is the full-size prototype of the radiofrequency (RF) ion source for ITER Neutral Beam Injector (NBI), with beam energy of 100keV, designed to operate with pulse length up to 3600 s, featuring ITER-like filter field configuration, caesium oven layout and a wide set of diagnostics. These features will allow reproducing the ion source operation like in ITER as cannot be done in any other existing test facility. SPIDER realization is well advanced and the first operation is expected at the beginning of 2018, with the mission to prove the possibility of achieving the ITER NBI ion source target requirements and of improving its performance in terms of reliability and availability. This paper mainly focuses on the preparation of the first SPIDER operations: integration and testing of SPIDER components, completion and implementation of diagnostics and control and workout of operation and research plan, based on a staged strategy.

The MITICA experiment (Megavolt ITER Injector & Concept Advancement) is the prototype and the test bed of the Heating and Current Drive Neutral Beam Injectors, which will be necessary for the full-performance exploitation of ITER. MITICA injector experiments shall demonstrate the reliable and accurate emission of a 17 MW beam of neutral particles for duration up to 1 hour, fulfilling ITER specific requirements. The accelerator grids are among the most critical parts of this experiment, because they must fulfill several operational requirements and at the same time satisfy the fatigue verifications according to the ITER Structural Design Criteria for In-vessel Components (SDC-IC). After about two years of continuous development, two design improvements were found to effectively increase the fatigue life of the grids up to the requested values. The first method was to adopt a novel shape of the cooling channels inside the grids, called Nozzle Island Cooling Enhancement (NICE) and able to provide a high performance cooling without exceeding the limits on the pressure drop through the grids. The second, called Stress Relieving Slits (SRS), was to introduce suitable slits in the grids, whose design was iteratively optimized until they were able to significantly reduce the stress/strain peaks due to thermal gradients. The NICE and SRS design solutions, here described in detail, were found to be key improvements in order to obtain a design of the grids able to satisfy all the operating requirements and all the structural verifications according to the ITER criteria.

The MITICA experiment (Megavolt ITER Injector & Concept Advancement) is the prototype and the test bed of the Heating and Current Drive Neutral Beam Injectors (HNB), which will be necessary for the full-performance exploitation of ITER. MITICA injector experiments shall demonstrate the reliable and accurate emission of a 17 MW beam of neutral particles of deuterium or hydrogen for duration up to 1 hour, fulfilling ITER specific requirements. MITICA test bed is in the final design phase and will be procured and installed in PRIMA facility (Padova Research on Injector Megavolt Accelerated) in Padova, Italy. The beam source is the key component of the system, as its goal is the generation of the 1 MeV accelerated beam of deuterium or hydrogen negative ions. The beam source is a complex system, having overall dimensions in the order of 4x4x3 m3 and a weight of around 20 tons. A major effort has been put in place in the recent months for the design closure, in collaboration with ITER Organization, F4E and European and Japanese laboratories (IPP, CEA, JAEA), solving the remaining outstanding issues, namely the optimization of the thermo-mechanical behavior of the accelerator grid segments subjected to a substantial heat power deposition, the finalization of the mechanical structure of the accelerator coherently identifying a reliable assembly and alignment procedure, the completion of the design of all involved interfaces with surrounding components and maintenance tools, and the conclusion of last R&D campaign on large ceramic insulators. Comprehensive analyses have also been carried out to finally verify the structural and electrostatic design of the overall structure with regard to normal operation, main fault conditions and seismic events. This paper presents the highlights of the latest developments for the finalization of the MITICA beam source design, together with a description of the most recent analyses and R&D activities carried out in support of the design.

The ITER project requires additional heating by two neutral beam injectors, each accelerating to 1 MV a 40 A beam of negative deuterium ions, to deliver to the plasma a power of about 17 MW for one hour. As these requirements have never been experimentally met, it was recognized as necessary to setup a test facility, PRIMA (Padova Research on ITER Megavolt Accelerator), in Italy, including a full-size negative ion source, SPIDER, and a prototype of the whole ITER injector, MITICA, aiming to develop the heating injectors to be installed in ITER. This realization is made with the main contribution of the European Union, through the Joint Undertaking for ITER (F4E), the ITER Organization and Consorzio RFX which hosts the Test Facility. The Japanese and the Indian ITER Domestic Agencies (JADA and INDA) participate in the PRIMA enterprise; European laboratories, such as IPP-Garching, KIT-Karlsruhe, CCFE-Culham, CEA-Cadarache and others are also cooperating. Presently, the assembly of SPIDER is on-going and the MITICA design is being completed. The paper gives a general overview of the test facility and of the status of development of the MITICA and SPIDER main components at this important stage of the overall development; then it focuses on the latest and most critical issues, regarding both physics and technology, describing the identified solutions.

The megavolt ITER injector and concept advancement experiment is the prototype and the test bed of the ITER heating and current drive neutral beam injectors, currently in the final design phase, in view of the installation in Padova Research on Injector Megavolt Accelerated facility in Padova, Italy. The beam source is the key component of the system, as its goal is the generation of the 1 MeV accelerated beam of deuterium or hydrogen negative ions. This paper presents the highlights of the latest developments for the finalization of the MITICA beam source design, together with a description of the most recent analyses and R&D activities carried out in support of the design.

The ITER Neutral Beam Test Facility (PRIMA) is presently under construction at Consorzio RFX (Padova, Italy). PRIMA includes two experimental devices: an ITER-size ion source with low voltage extraction, called SPIDER, and the full prototype of the whole ITER Heating Neutral Beams (HNBs), called MITICA. The purpose of MITICA is to demonstrate that all operational parameters of the ITER HNB accelerator can be experimentally achieved, thus establishing a large step forward in the performances of neutral beam injectors in comparison with the present experimental devices. The design of the MITICA extractor and accelerator grids, here described in detail, was developed using an integrated approach, taking into consideration at the same time all the relevant physics and engineering aspects. Particular care was taken also to support and validate the design on the basis of the expertise and experimental data made available by the collaborating neutral beam laboratories of CEA, IPP, CCFE, NIFS and JAEA. Considering the operational requirements and the other physics constraints of the ITER HNBs, the whole design has been thoroughly optimized and improved. Furthermore, specific innovative concepts have been introduced.

To reach fusion conditions and control plasma configuration in ITER, a suitable combination of additional heating and current drive systems is necessary. Among them, two Neutral Beam Injectors (NBI) will provide 33 MW hydrogen/deuterium particles electrostatically accelerated to 1 MeV; efficient gas-cell neutralisation at such beam energy requires negative ions, obtained by caesium-catalysed surface conversion of atoms inside the ion source. As ITER NBI requirements have never been simultaneously attained, a Neutral Beam Test Facility (NBTF) was set up at Consorzio RFX (Italy), including two experiments. MITICA is the full-scale NBI prototype with 1 MeV particle energy. SPIDER, with 100 keV particle energy, aims at testing and optimising the full-scale ion source: extracted beam uniformity, negative ion current density (for one hour) and beam optics (beam divergence

Several novel design solutions for high performance cooling systems have been developed by Consorzio RFX, permitting to simulate the challenging heat transfer conditions foreseen in the future fusion devices. The project, called Multi-design Innovative Cooling Research & Optimization (MICRO), aims, on one hand, to verify the present solution applied inside the NBI accelerator grids (i.e. MITICA experiment) and, on the other, to perform further improvements in the heat transfer process with an acceptable pressure drop and reliable manufacturing process. The main advantages rely on the possibility to extend the fatigue life-cycle of different high thermal stress components and to investigate the possibility to employ alternative dielectric fluids instead of water. Such design solutions would in fact allow the exploitation of less performing fluids in terms of cooling capability. This is particularly relevant in view of DEMO and future power plants characterized by higher efficiency and reliability. The paper gives a description of the Computation Fluid Dynamics (CFD) analysis and the samples manufacturing process.

The measurements of the magnetic field in tokamaks such as ITER and DEMO will be challenging due to the long pulse duration, high neutron flux, and elevated temperatures. The long duration of the plasma pulse makes standard techniques, such as inductive coils, prone to errors. At the same time, the hostile environment, with repairs possible only on blanket exchange, if at all, requires a robust magnetic sensor. This contribution presents the final design of novel, steady-state, magnetic sensors for ITER. A poloidal array of 60 sensors mounted on the vacuum vessel outer shell contributes to the measurement of the plasma current, plasma-wall clearance, low-frequency MHD modes and will allow for crosscheck with the outer-vessel inductive coils. Each sensor hosts a pair of bismuth Hall probes, themselves an outcome of extensive R&D, including neutron irradiations (to 10 23 n/m 2 ), temperature cycling tests (73-473 K) and tests at high magnetic field (to 12 T). A significant effort has been devoted to optimize the sensor housing by design and prototyping. The production version features an indium-filled cell for in situ recalibration of the onboard thermocouple, vital for the interpretation of the Hall sensor measurement.