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299 results on '"Fukao, Yoichiro"'

1. An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function

Author

Li, Yuying, Wang, Qiong, Jia, Huimin, Ishikawa, Kazuya, Kosami, Ken-ichi, Ueba, Takahiro, Tsujimoto, Atsumi, Yamanaka, Miki, Yabumoto, Yasuyuki, Miki, Daisuke, Sasaki, Eriko, Fukao, Yoichiro, Fujiwara, Masayuki, Kaneko-Kawano, Takako, Tan, Li, Kojima, Chojiro, Wing, Rod A., Sebastian, Alfino, Nishimura, Hideki, Fukada, Fumi, Niu, Qingfeng, Shimizu, Motoki, Yoshida, Kentaro, Terauchi, Ryohei, Shimamoto, Ko, and Kawano, Yoji

Published
2024
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7. Histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 proteins affect plant growth under nitrogen deficient conditions in Arabidopsis thaliana

Author

Jie, Linnan, Sanagi, Miho, Luo, Yongming, Maeda, Haruna, Fukao, Yoichiro, Chiba, Yukako, Yanagisawa, Shuichi, Yamaguchi, Junji, Takagi, Junpei, and Sato, Takeo

Subjects
histone chaperone, nutrient, Arabidopsis, gene expression, nitrogen
Abstract

Nitrogen (N) availability is one of the most important factors regulating plant metabolism and growth as it affects global gene expression profiles. Dynamic changes in chromatin structure, including histone modifications and nucleosome assembly/disassembly, have been extensively shown to regulate gene expression under various environmental stresses in plants. However, the involvement of chromatin related changes in plant nutrient responses has been demonstrated only in a few studies to date. In this study, we investigated the function of histone chaperone NUCLEOSOME ASSEMBLY PROTEIN1 (NAP1) proteins under N deficient conditions in Arabidopsis. In the nap1;1 nap1;2 nap1;3 triple mutant (m123-1), the expression of N-responsive marker genes and growth of lateral roots were decreased under N deficient conditions. In addition, the m123-1 plants showed a delay in N deficiency-induced leaf senescence. Taken together, these results suggest that NAP1s affect plant growth under N deficient conditions in Arabidopsis.

Published
2023

15. TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics

Author

Zhu, Jinsheng, Bailly, Aurelien, Zwiewka, Marta, Sovero, Valpuri, Di Donato, Martin, Ge, Pei, Oehri, Jacqueline, Aryal, Bibek, Hao, Pengchao, Linnert, Miriam, Burgardt, Noelia Inés, Lücke, Christian, Weiwad, Matthias, Michel, Max, Weiergräber, Oliver H., Pollmann, Stephan, Azzarello, Elisa, Mancuso, Stefano, Ferro, Noel, Fukao, Yoichiro, Hoffmann, Céline, Wedlich-Söldner, Roland, Friml, Jiří, Thomas, Clément, and Geisler, Markus

Published
2016

18. The endoplasmic reticulum membrane–bending protein RETICULON facilitates chloroplast relocation movement in Marchantia polymorpha

Author

Ishikawa, Kazuya, Konno, Ryota, Hirano, Satoyuki, Fujii, Yuta, Fujiwara, Masayuki, Fukao, Yoichiro, and Kodama, Yutaka

Subjects
葉緑体, Phototropins, Chloroplasts, ゼニゴケ, Light, 小胞体, Marchantia, フォトトロピン, Endoplasmic Reticulum, 光
Abstract

text, 学術雑誌論文 / Journal Article, Plant cells alter the intracellular positions of chloroplasts to ensure efficient photosynthesis, a process controlled by the blue light receptor phototropin. Chloroplasts migrate toward weak light (accumulation response) and move away from excess light (avoidance response). Chloroplasts are encircled by the endoplasmic reticulum (ER), which forms a complex network throughout the cytoplasm. To ensure rapid chloroplast relocation, the ER must alter its structure in conjunction with chloroplast relocation movement, but little is known about the underlying mechanism. Here, we searched for interactors of phototropin in the liverwort Marchantia polymorpha and identified a RETICULON (RTN) family protein; RTN proteins play central roles in ER tubule formation and ER network maintenance by stabilizing the curvature of ER membranes in eukaryotic cells. Marchantia polymorpha RTN1 (MpRTN1) is localized to ER tubules and the rims of ER sheets, which is consistent with the localization of RTNs in other plants and heterotrophs. The Mprtn1 mutant showed an increased ER tubule diameter, pointing to a role for MpRTN1 in ER membrane constriction. Furthermore, Mprtn1 showed a delayed chloroplast avoidance response but a normal chloroplast accumulation response. The live cell imaging of ER dynamics revealed that ER restructuring was impaired in Mprtn1 during the chloroplast avoidance response. These results suggest that during the chloroplast avoidance response, MpRTN1 restructures the ER network and facilitates chloroplast movement via an interaction with phototropin. Our findings provide evidence that plant cells respond to fluctuating environmental conditions by controlling the movements of multiple organelles in a synchronized manner.

Published
2022

23. Zinc deficiency‐induced defensin‐like proteins are involved in the inhibition of root growth in Arabidopsis.

Author

Kimura, Sachie, Vaattovaara, Aleksia, Ohshita, Tomoya, Yokoyama, Kotomi, Yoshida, Kota, Hui, Agnes, Kaya, Hidetaka, Ozawa, Ai, Kobayashi, Mami, Mori, Izumi C., Ogata, Yoshiyuki, Ishino, Yoko, Sugano, Shigeo S., Nagano, Minoru, and Fukao, Yoichiro

Subjects
ROOT growth, PLANT genomes, ZINC, PLANT adaptation, PLANT growth, PROMOTERS (Genetics)
Abstract

SUMMARY: The depletion of cellular zinc (Zn) adversely affects plant growth. Plants have adaptation mechanisms for Zn‐deficient conditions, inhibiting growth through the action of transcription factors and metal transporters. We previously identified three defensin‐like (DEFL) proteins (DEFL203, DEFL206 and DEFL208) that were induced in Arabidopsis thaliana roots under Zn‐depleted conditions. DEFLs are small cysteine‐rich peptides involved in defense responses, development and excess metal stress in plants. However, the functions of DEFLs in the Zn‐deficiency response are largely unknown. Here, phylogenetic tree analysis revealed that seven DEFLs (DEFL202–DEFL208) were categorized into one subgroup. Among the seven DEFLs, the transcripts of five (not DEFL204 and DEFL205) were upregulated by Zn deficiency, consistent with the presence of cis‐elements for basic‐region leucine‐zipper 19 (bZIP19) or bZIP23 in their promoter regions. Microscopic observation of GFP‐tagged DEFL203 showed that DEFL203‐sGFP was localized to the apoplast and plasma membrane. Whereas a single mutation of the DEFL202 or DEFL203 genes only slightly affected root growth, defl202 defl203 double mutants showed enhanced root growth under all growth conditions. We also showed that the size of the root meristem was increased in the double mutants compared with the wild type. Our results suggest that DEFL202 and DEFL203 are redundantly involved in the inhibition of root growth under Zn‐deficient conditions through a reduction in root meristem length and cell number. Significance Statement: Defensins are small cysteine‐rich peptides, and a plant genome contains a large number of defensin‐like (DEFL) genes; however, their function remains unclear. Here, we showed that five DEFL genes were upregulated in roots under Zn‐depleted conditions, and that DEFL202 and DEFL203 might function as inhibitors of root growth by modulating root meristem length and cell number. [ABSTRACT FROM AUTHOR]

Published
2023
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25. The Arabidopsis CERK1‐associated kinase PBL27 connects chitin perception to MAPK activation

Author

Yamada, Kenta, Yamaguchi, Koji, Shirakawa, Tomomi, Nakagami, Hirofumi, Mine, Akira, Ishikawa, Kazuya, Fujiwara, Masayuki, Narusaka, Mari, Narusaka, Yoshihiro, Ichimura, Kazuya, Kobayashi, Yuka, Matsui, Hidenori, Nomura, Yuko, Nomoto, Mika, Tada, Yasuomi, Fukao, Yoichiro, Fukamizo, Tamo, Tsuda, Kenichi, Shirasu, Ken, Shibuya, Naoto, and Kawasaki, Tsutomu

Published
2016
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39. HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids in Chlamydomonas reinhardtii

Author

Takusagawa, Mari, Kobayashi, Yusuke, Fukao, Yoichiro, Hidaka, Kumi, Endo, Masayuki, Sugiyama, Hiroshi, Hamaji, Takashi, Kato, Yoshinobu, Miyakawa, Isamu, Misumi, Osami, Shikanai, Toshiharu, and Nishimura, Yoshiki

Subjects
chloroplast nucleoid, mitochondrial nucleoid, HMG-box domain
Abstract

Compaction of bulky DNA is a universal issue for all DNA-based life forms. Chloroplast nucleoids (chloroplast DNA–protein complexes) are critical for chloroplast DNA maintenance and transcription, thereby supporting photosynthesis, but their detailed structure remains enigmatic. Our proteomic analysis of chloroplast nucleoids of the green alga Chlamydomonas reinhardtii identified a protein (HBD1) with a tandem repeat of two DNA-binding high mobility group box (HMG-box) domains, which is structurally similar to major mitochondrial nucleoid proteins transcription factor A, mitochondrial (TFAM), and ARS binding factor 2 protein (Abf2p). Disruption of the HBD1 gene by CRISPR-Cas9–mediated genome editing resulted in the scattering of chloroplast nucleoids. This phenotype was complemented when intact HBD1 was reintroduced, whereas a truncated HBD1 with a single HMG-box domain failed to complement the phenotype. Furthermore, ectopic expression of HBD1 in the mitochondria of yeast Δabf2 mutant successfully complemented the defects, suggesting functional similarity between HBD1 and Abf2p. Furthermore, in vitro assays of HBD1, including the electrophoretic mobility shift assay and DNA origami/atomic force microscopy, showed that HBD1 is capable of introducing U-turns and cross-strand bridges, indicating that proteins with two HMG-box domains would function as DNA clips to compact DNA in both chloroplast and mitochondrial nucleoids., 葉緑体核様体をコンパクトに折りたたむ「DNAクリップ」の発見 --ミトコンドリアとも共通する普遍的なしくみの解明--. 京都大学プレスリリース. 2021-05-12.

Published
2021

47. TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis.

Author

Hasegawa, Yoko, Reyes, Thais Huarancca, Uemura, Tomohiro, Baral, Anirban, Fujimaki, Akari, Luo, Yongming, Morita, Yoshie, Saeki, Yasushi, Maekawa, Shugo, Yasuda, Shigetaka, Mukuta, Koki, Fukao, Yoichiro, Tanaka, Keiji, Nakano, Akihiko, Takagi, Junpei, Bhalerao, Rishikesh P, Yamaguchi, Junji, and Sato, Takeo

Published
2022
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