1. Fucodiphlorethol G Purified from Ecklonia cava Suppresses Ultraviolet B Radiation-Induced Oxidative Stress and Cellular Damage
- Author
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Jin Won Hyun, Ji Won Cha, Jian Zheng, Ki Cheon Kim, Madduma Hewage Susara Ruwan Kumara, Nam Ho Lee, Xia Han, Hee Kyoung Kang, Mei Jing Piao, and Cheng Wen Yao
- Subjects
Ecklonia cava ,Radical ,Oxidative phosphorylation ,Mitochondria membrane potential ,medicine.disease_cause ,Biochemistry ,Drug Discovery ,Botany ,medicine ,Cell damage ,Pharmacology ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,integumentary system ,Human keratinocytes ,biology.organism_classification ,medicine.disease ,Molecular biology ,chemistry ,Apoptosis ,Molecular Medicine ,DNA fragmentation ,Original Article ,Ultraviolet B ,Oxidative stress ,Fucodiphlorethol G - Abstract
Fucodiphlorethol G (6'-[2,4-dihydroxy-6-(2,4,6-trihydroxyphenoxy)phenoxy]biphenyl-2,2',4,4',6-pentol) is a compound purified from Ecklonia cava, a brown alga that is widely distributed offshore of Jeju Island. This study investigated the protective effects of fucodiphlorethol G against oxidative damage-mediated apoptosis induced by ultraviolet B (UVB) irradiation. Fucodiphlorethol G attenuated the generation of 2, 2-diphenyl-1-picrylhydrazyl radicals and intracellular reactive oxygen species in response to UVB irradiation. Fucodiphlorethol G suppressed the inhibition of human keratinocyte growth by UVB irradiation. Additionally, the wavelength of light absorbed by fucodiphlorethol G was close to the UVB spectrum. Fucodiphlorethol G reduced UVB radiation-induced 8-isoprostane generation and DNA fragmentation in human keratinocytes. Moreover, fucodiphlorethol G reduced UVB radiation-induced loss of mitochondrial membrane potential, generation of apoptotic cells, and active caspase-9 expression. Taken together, fucodiphlorethol G protected human keratinocytes against UVB radiation-induced cell damage and apoptosis by absorbing UVB radiation and scavenging reactive oxygen species.
- Published
- 2014