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The purpose of this paper was to report, for first time, the presence of Ochrobactrum anthropi as contaminant of in vitro cell and tissue culture of sugarcane. Bacteria contaminant of in vitro plants were isolated directly from visibly contaminated plant culture on Tryptone soy agar medium and some drops of sugarcane cell suspension contaminated were inoculated on tubes with 3ml of Tryptone Soy Broth (TSB). Isolated bacteria were examined microscopically (shape, motility) and some biochemical test (Gram's stain (Hucker's modification), oxidase, catalase, O/F (oxidation/fermentation), were performed. Additional tests, according to Bergey's Manual of Determinative Bacteriology, were performed. Identification was complemented by fatty acid analysis. Different bacteria can be contaminating plant cell and tissue culture. However, references about the presence of Ochrobactrum anthropi as contaminant of in vitro culture of plant cell and tissue have not been reached. to control microbial contaminants, it is necessary to identify them. [ABSTRACT FROM AUTHOR]

The low multiplication and ex vitro survival rates during acclimatization in the culture house limit the in vitro mass propagation of B. vulgaris. Several scientific studies have described the development of different protocols for bamboo; however, not all of them address the effects of these systems on plant morphology, physiology, and biochemistry in vitro. In this chapter, a complete and optimized protocol is described for plants propagated via organogenesis in temporary immersion systems. In addition, the morphophysiological and biochemical characterization of the plants as well as the survival rates of the obtained plants under ex vitro conditions are analyzed. The obtained results will be the basis for the development of a technology for in vitro propagation as an alternative for the production of plants of the species., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)