19 results on '"FIGUEIREDO, F. B."'
Search Results
2. Trypanosoma caninum n. sp. (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil
- Author
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MADEIRA, M. F., SOUSA, M. A., BARROS, J. H. S., FIGUEIREDO, F. B., FAGUNDES, A., SCHUBACH, A., DE PAULA, C. C., FAISSAL, B. N. S., FONSECA, T. S., THOMA, H. K., and MARZOCHI, M. C. A.
- Published
- 2009
3. Mycological evaluation of bronchoalveolar lavage in cats with respiratory signs from Rio de Janeiro, Brazil
- Author
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Leme, L. R. P., Schubach, T. M. P., Santos, I. B., Figueiredo, F. B., Pereira, S. A., Reis, R. S., Mello, M. F. V., Ferreira, A. M. R., Quintella, L. P., and Schubach, A. O.
- Published
- 2007
4. Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis.
- Author
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Campos, M. P., Madeira, M. F., Silva, D. A., Solcà, M. S., Espíndola, O.M., Júnior, A. A. V. Mendes, and Figueiredo, F. B.
- Abstract
Copyright of Arquivo Brasileiro de Medicina Veterinaria e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
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5. Response to azolic antifungal agents for treating feline sporotrichosis.
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Pereira, S. A., Passos, S. R. L., Silva, J. N., Gremião, I. D. F., Figueiredo, F. B., Teixeira, J. L., Monteiro, P. C. F., and Schubach, T. M. P.
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ANTIFUNGAL agents ,KETOCONAZOLE ,SPOROTRICHOSIS ,TREATMENT of cat diseases ,COMMUNICABLE disease treatment ,MYCOSES ,VETERINARY medicine ,THERAPEUTICS - Abstract
The effectiveness and safety of treatment with ketoconazole and itraconazole were compared in 773 sporotrichosis-infected cats over a four-year period (2002 to 2005). Five hundred and ninety-eight cats received oral ketoconazole and 175 received oral itraconazole. Treatment was successful in 238 (30.8 per cent) cats, of which 171 (28.6 per cent) of 598 received 13.5 to 27.0 mg/kg/day ketoconazole and 67 (38.3 per cent) of 175 received 8.3 to 27.7 mg/kg/day itraconazole. Adverse effects were reported in 306 (39.6 per cent) of the cats, 105 (13.6 per cent) died and 430 (55.6 per cent) dropped out of treatment or were still under treatment at the time of data analysis. [ABSTRACT FROM AUTHOR]
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- 2010
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6. Isolation of Trypanosoma caninum in domestic dogs in Rio de Janeiro, Brazil.
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Pinto, A. G. De S., Schubach, T. M. P., Figueiredo, F. B., Baptista, C., Fagundes, A., Barros, J. H. Da S., De Paula, C. C., Toma, H. K., and Madeira, M. F.
- Subjects
TRYPANOSOMATIDAE ,LEISHMANIASIS ,ISOENZYMES ,POLYMERASE chain reaction ,TRYPANOSOMA brucei ,ELECTROPHORESIS ,RIBOSOMAL DNA - Abstract
The domestic dog's involvement with different members of the Trypanosomatidae family has been the focus of several studies due to this animal's close proximity to man. Recently this animal has been infected by a new Trypanosoma species (T. caninum), described in Rio de Janeiro and 19 similar isolates were later obtained. The objective of this study was to identify these isolates. All samples were isolated from intact skin cultures and analysed morphologically, by biochemical isoenzyme electrophoresis assays and by several molecular PCR assays. Additionally, anti-Leishmania sp. antibodies were assessed using the indirect Immunofluorescence Antibody Test (IFAT) in all animals. The methodologies employed to identify the isolates, including partial nucleotide sequences of 18S rRNA gene, indicated patterns identical to T. caninum and patterns different from the other species, including T. cruzi and T. rangeli samples. A phylogenetic tree constructed with the partial 18S ribosomal sequence shows that T. caninum is clustered with T. pestanai. Ten (52·6%) animals presented anti-Leishmania sp. antibodies with titres varying from 1 : 40 to 1 : 320. Thus, the hypothesis that this protozoan has disseminated among the dogs in Rio de Janeiro must be considered. The importance of a correct diagnosis in those animals and the possible consequences in the areas where visceral leishmaniasis is found are discussed here. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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- View/download PDF
7. Efficacy of an indirect immunofluorescence test in the diagnosis of canine leishmaniosis.
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Figueiredo, F. B., Madeira, M. F., Menezes, R. C., Pacheco, R. S., Pires, M. Q., Furtado, M. C., Pinto, A. G., and Schubach, T. M. P.
- Subjects
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IMMUNOFLUORESCENCE , *LEISHMANIASIS , *VISCERAL leishmaniasis , *SEROLOGY , *DOG diseases - Abstract
Of 146 dogs from a visceral leishmaniosis-endemic area that tested seronegative by indirect immunofluorescence (IIF) on blood samples collected on filter paper (IIFp), 51 (34.9%) and 10 (6.8%) tested positive by IIF on serum samples (IIFs) and enzyme immunoassay, respectively. Three samples (2.0%) tested positive by PCR. Leishmania chagasi was isolated from the skin of five (3.4%) dogs. Amastigote forms were identified in two of these five animals following histopathological and immunohistochemical examination. The findings highlight that detection methods such as IIFp can permit dogs infected with L. chagasi to remain undetected in endemic areas with attendant consequences for the epidemiology of infection both in the canine and human populations. [ABSTRACT FROM AUTHOR]
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- 2010
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8. Cell-block Immunohistochemistry of Bone Marrow Aspirates: a Novel Tool to Improve the Diagnosis of Leishmania Infection in Dogs.
- Author
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Menezes RC, Madeira MF, Ferreira LC, Barbosa Filho CJ, Miranda LH, and Figueiredo FB
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- Animals, Biopsy, Needle, Bone Marrow pathology, Dogs, Leishmania infantum, Leishmaniasis, Visceral diagnosis, Bone Marrow parasitology, Dog Diseases diagnosis, Immunohistochemistry methods, Leishmaniasis, Visceral veterinary
- Abstract
Parasitological methods are the most specific procedures used for the diagnosis of Leishmania spp. infection, but their limited sensitivity poses a disadvantage and prompts the need for alternatives. The choice of site for sample collection influences diagnostic sensitivity. The combination of an accurate diagnostic method and a technique that allows large-scale field studies is highly desirable to enhance the investigation of Leishmania spp. infection in dogs, especially in endemic regions. The bone marrow is a good target for the detection of Leishmania spp. in dogs. In this context, bone marrow aspiration is rapid and less invasive compared with biopsy procedures, and also enables cell block processing, paraffin wax embedding and the sectioning of samples for further histological and immunohistochemical analyses. The aim of this study was to describe for the first time parasitological methods (immunohistochemistry [IHC] and histopathology) using the cell block technique with bone marrow aspirates for the diagnosis of Leishmania spp. infection in dogs. Bone marrow aspiration was performed in 45 dogs from an area endemic for visceral leishmaniosis for parasitological culture and the cell block technique (histopathology and IHC). Fourteen (31.1%) dogs tested positive for Leishmania spp. by IHC, six (13.3%) by parasitological culture and four (8.9%) by histopathology. Cell block IHC was a useful tool for the diagnosis of canine visceral leishmaniosis. Further studies should be conducted to validate this method for routine epidemiological screening., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
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- 2016
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9. Evaluation of 18S rDNA PCR assay using skin fragments as a diagnostic test for Trypanosoma caninum.
- Author
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Pinto AG, Toma HK, Figueiredo FB, and Madeira MF
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- Animals, DNA, Protozoan genetics, DNA, Ribosomal genetics, Dog Diseases parasitology, Dogs, Leishmania genetics, Leishmania isolation & purification, Leishmaniasis diagnosis, Leishmaniasis parasitology, RNA, Ribosomal, 18S genetics, Skin parasitology, Trypanosoma genetics, Trypanosomiasis diagnosis, Trypanosomiasis parasitology, Dog Diseases diagnosis, Leishmaniasis veterinary, Polymerase Chain Reaction veterinary, Trypanosoma isolation & purification, Trypanosomiasis veterinary
- Abstract
Trypanosoma caninum is a new species that has been recently identified in Brazil and infects domestic dogs. To date, no accurate diagnostic assays for this parasite have been established; thus, our aim was to evaluate more than one type of PCR for the diagnosis and molecular screening of T. caninum in 229 dogs living in Rio de Janeiro state. The tests were based on the amplification and sequencing of the 18S ribosomal DNA (rDNA) gene using healthy skin fragments. Additionally, PCR amplification of the kDNA minicircles region specific to the Leishmania genus was performed. The PCR results were compared with those of culture-based analysis performed with the same specimen. Using cultures, T. caninum and Leishmania chagasi were isolated from 11 and 12 dogs, respectively, whereas the 18S rDNA PCR assay detected parasitic infection in 35 dogs. Among these, 25 dogs showed an amplification pattern similar to T. caninum and 10 showed a pattern similar to L. chagasi; these results were confirmed by sequencing analysis. The kDNA PCR analysis showed that 14 dogs were positive for Leishmania infection. Of these, 2 dogs showed negative culture results and 12 were positive for L. chagasi, including 4 with negative 18S rDNA PCR results. Thus far, culture-based testing has been the only tool used successfully for T. caninum diagnosis. Our results demonstrate that 18S rDNA PCR-based test should be a useful diagnostic tool, particularly for distinguishing between T. caninum and L. chagasi infections in areas where these 2 parasites co-exist., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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10. Natural breeding sites of phlebotomine sand flies (Diptera: Psychodidae) on Marambaia Island, Rio de Janeiro State, Brazil.
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Souza TL, Figueiredo FB, Almeida AB, Benigno CV, Pontes CS, and Souza MB
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- Animals, Brazil, Reproduction physiology, Species Specificity, Ecosystem, Islands, Psychodidae physiology
- Abstract
Immature phlebotomine sand flies develop in soils with essential and ideal characteristics for their life cycle, such as organic matter, humidity, temperature and low levels of light. Information regarding the potential breeding places of these dipterans is fundamental to understand the epidemiology and ecology of leishmaniasis, in addition to its importance to control them. In the present study, we aimed to find natural breeding sites of sand flies on Marambaia Island with the aid of emergence traps and direct search of immature forms using the flotation technique with saturated sugar solution in organic substrates of the region. Both methods were effective, with a total of 42 specimens of six different species - including some species that participate in the transmission cycle of American Tegumentary Leishmaniasis - collected by the emergence traps, and five immature forms obtained by floatation technique. However, further studies are still necessary, mainly with respect to the ecology and biology of immature sandfly stages, so that control measures focused on breeding sites can produce positive sustainable results in natural environments., (Copyright © 2014 Elsevier B.V. All rights reserved.)
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- 2014
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11. Evaluation of serological cross-reactivity between canine visceral leishmaniasis and natural infection by Trypanosoma caninum.
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Alves AS, Mouta-Confort E, Figueiredo FB, Oliveira RV, Schubach AO, and Madeira MF
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- Animals, Antibodies, Protozoan blood, Cross Reactions immunology, Dog Diseases diagnosis, Dogs, Leishmaniasis, Visceral blood, Leishmaniasis, Visceral diagnosis, Trypanosoma immunology, Trypanosomiasis blood, Trypanosomiasis diagnosis, Dog Diseases parasitology, Leishmaniasis, Visceral veterinary, Serologic Tests veterinary, Trypanosoma classification, Trypanosomiasis veterinary
- Abstract
In order to evaluate if the presence of Trypanosoma caninum can lead to a confuse diagnosis of canine visceral leishmaniasis (CVL), we investigated the serological status of dogs infected by T. caninum and assessed the serological cross-reactivity with CVL. A set of 117 serum samples from dogs infected by T. caninum, Leishmania chagasi and not infected dogs (n=39 in each group) was tested using commercial kits--indirect immunofluorescence (IFI-LVC), ELISA (EIE-LVC) and immunochromatographic test (DPP)--and in house tests with T. caninum (IIF-Tc and ELISA-Tc) and L. chagasi antigens (IIF-Lc and ELISA-Lc). IIF-Tc and ELISA-Tc presented sensitivity of 64.1% and 94.9% and specificity of 23.1% and 35.9%, respectively. The sensitivity of the IFI-LVC, EIE-LVC and DPP tests was 100% and the specificity was 70.5%, 68% and 97.5% respectively. The concordance between the tests was considered as satisfactory. The specificities of IFI-LVC, EIE-LVC and DPP were higher when the group Tc was excluded, with significant values for IFI-LVC (χ2=4.36, P-value=0.036), thus suggesting that the infection by T. caninum can confuse the diagnosis of CVL., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
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12. Occurrence of Trypanosoma caninum in areas overlapping with leishmaniasis in Brazil: what is the real impact of canine leishmaniasis control?
- Author
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Barros JH, Almeida AB, Figueiredo FB, Sousa VR, Fagundes A, Pinto AG, Baptista C, and Madeira MF
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- Animals, Brazil epidemiology, Chagas Disease epidemiology, Chagas Disease prevention & control, Communicable Disease Control, Dog Diseases prevention & control, Dogs, Endemic Diseases veterinary, Euthanasia, Animal, Humans, Leishmania infantum genetics, Leishmaniasis epidemiology, Leishmaniasis prevention & control, Polymerase Chain Reaction methods, Sequence Analysis, DNA, Trypanosoma genetics, Chagas Disease veterinary, Dog Diseases epidemiology, Leishmania infantum isolation & purification, Leishmaniasis veterinary, RNA, Ribosomal, 18S isolation & purification, Trypanosoma isolation & purification
- Abstract
Trypanosoma caninum is a parasite of the Trypanosoma genus recently described in the natural infection of dogs in the municipality of Rio de Janeiro, Brazil. Suspecting the existence of a natural cycle and the circulation of this new species, the objective of this study was the taxonomic identification of samples of Trypanosoma spp. isolated from dogs in different Brazilian regions. Parasites were solely obtained from skin fragments culture and characterized by nested-PCR targeting the partial sequence of 18S rRNA gene and PCR products were sequenced. Thirty-three samples, obtained in São Paulo, Minas Gerais, Goiás, Mato Grosso and Rio de Janeiro states were analyzed. PCR and sequencing showed that the isolates were genetically identical or closely similar and confirmed T. caninum identity. This report broadens the geographical distribution of T. caninum in Brazil and discusses the impact of the presence of this parasite in areas of canine leishmaniasis occurrence., (Copyright © 2012 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.)
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- 2012
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13. Laboratory tests performed on Leishmania seroreactive dogs euthanized by the leishmaniasis control program.
- Author
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Silva DA, Madeira MF, Teixeira AC, de Souza CM, and Figueiredo FB
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- Animals, Brazil epidemiology, Communicable Disease Control organization & administration, Dogs, Endemic Diseases veterinary, Leishmaniasis blood, Leishmaniasis prevention & control, Dog Diseases blood, Euthanasia, Animal, Leishmania immunology, Leishmaniasis veterinary
- Abstract
In 2008, in the west zone of Rio de Janeiro municipality-Brazil, the leishmaniasis control program identified 155 dogs with titers ≥ 40 by Indirect ImmunoFluorescence (IIF) on blood collected onto filter paper. The objective of this study was to describe the laboratory test findings performed in dogs euthanized by the leishmaniasis program control of Rio de Janeiro municipality. Dogs were examined, subjected to euthanasia and collection of clinical specimens. Parasite isolation was obtained in 29 animals: Leishmania chagasi was isolated in 14 dogs; Leishmania braziliensis was isolated in five dogs; Trypanosoma caninum was obtained in seven animals and one dog had mixed infection (L. braziliensis and L. chagasi). By Polymerase Chain Reaction, seventeen animals were positive in intact skin fragments. In the serological reassessment of serum samples, 28% and 22% were positive for IIF and enzyme immunoassay, respectively. Ninety-one (59%) dogs were negative for all tests performed in this study. The findings indicate that the visceral leishmaniasis control program needs to be adjusted in order to avoid non-infected dogs from being removed or permit that dogs infected with L. chagasi to remain undetected in endemic areas., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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14. Isolation of Trypanosoma caninum in domestic dogs in Rio de Janeiro, Brazil.
- Author
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De S Pinto AG, Schubach TM, Figueiredo FB, Baptista C, Fagundes A, Da S Barros JH, De Paula CC, Toma HK, and Madeira MF
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- Animals, Antibodies, Protozoan blood, Brazil epidemiology, Dog Diseases diagnosis, Dogs, Electrophoresis methods, Isoenzymes analysis, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction methods, RNA, Ribosomal, 18S genetics, Sequence Analysis, DNA, Skin parasitology, Trypanosoma classification, Trypanosoma enzymology, Trypanosomiasis diagnosis, Trypanosomiasis epidemiology, Trypanosomiasis parasitology, Animals, Domestic parasitology, Dog Diseases epidemiology, Dog Diseases parasitology, Trypanosoma genetics, Trypanosoma isolation & purification, Trypanosomiasis veterinary
- Abstract
Summary: The domestic dog's involvement with different members of the Trypanosomatidae family has been the focus of several studies due to this animal's close proximity to man. Recently this animal has been infected by a new Trypanosoma species (T. caninum), described in Rio de Janeiro and 19 similar isolates were later obtained. The objective of this study was to identify these isolates. All samples were isolated from intact skin cultures and analysed morphologically, by biochemical isoenzyme electrophoresis assays and by several molecular PCR assays. Additionally, anti-Leishmania sp. antibodies were assessed using the indirect Immunofluorescence Antibody Test (IFAT) in all animals. The methodologies employed to identify the isolates, including partial nucleotide sequences of 18S rRNA gene, indicated patterns identical to T. caninum and patterns different from the other species, including T. cruzi and T. rangeli samples. A phylogenetic tree constructed with the partial 18S ribosomal sequence shows that T. caninum is clustered with T. pestanai. Ten (52.6%) animals presented anti-Leishmania sp. antibodies with titres varying from 1:40 to 1:320. Thus, the hypothesis that this protozoan has disseminated among the dogs in Rio de Janeiro must be considered. The importance of a correct diagnosis in those animals and the possible consequences in the areas where visceral leishmaniasis is found are discussed here.
- Published
- 2010
- Full Text
- View/download PDF
15. Comparative histopathological study of sporotrichosis and American tegumentary leishmaniasis in dogs from Rio de Janeiro.
- Author
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Miranda LH, Quintella LP, Santos IB, Oliveira RV, Menezes RC, Figueiredo FB, and Schubach TM
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- Animals, Brazil, Diagnosis, Differential, Dog Diseases immunology, Dogs, Granuloma immunology, Granuloma pathology, Inflammation immunology, Inflammation pathology, Leishmaniasis, Cutaneous immunology, Leishmaniasis, Cutaneous pathology, Macrophages immunology, Macrophages pathology, Neutrophil Infiltration immunology, Odds Ratio, Skin immunology, Sporotrichosis immunology, Sporotrichosis pathology, Dog Diseases pathology, Leishmaniasis, Cutaneous veterinary, Skin pathology, Sporotrichosis veterinary
- Abstract
Pyogranulomatous lesions from 80 dogs with sporotrichosis and 26 dogs with American tegumentary leishmaniasis (ATL) were compared microscopically in order to identify features that would support the diagnostic suspicion and direct the subsequent search for the aetiological agent of either infection. Odds ratios and their respective 95% confidence intervals were calculated in order to evaluate the impact of the microscopical findings on the diagnosis of either disease. Lesions with well-formed granulomata were 14 times more likely to be due to sporotrichosis than ATL. Marked neutrophil infiltration into granulomata was 12.26 times more likely to be associated with sporotrichosis when compared with lesions having mild neutrophilic infiltration. Absence of lymphocytes and macrophages in the peripheral infiltrate was associated with a 9.71 and 4.93 higher chance, respectively, of being sporotrichosis rather than ATL compared with lesions where these cells were present. Lesions with a perivascular, perifollicular and interstitial peripheral inflammatory infiltrate were 5.48 times more likely to be due to sporotrichosis than ATL when compared with lesions with a diffuse peripheral infiltrate. Histopathological analysis may therefore contribute to the diagnosis of sporotrichosis or ATL skin lesions in dogs since this method permits the identification of features that direct the diagnostic suspicion, thus facilitating the search for the aetiological agent in histological sections, permitting the precise request of subsequent tests and thereby reducing costs and time taken to achieve a definitive diagnosis and the initiation of appropriate therapy., ((c) 2009 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
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16. Parasitological diagnosis of canine visceral leishmaniasis: is intact skin a good target?
- Author
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Madeira MF, Figueiredo FB, Pinto AG, Nascimento LD, Furtado M, Mouta-Confort E, de Paula CC, Bogio A, Gomes MC, Bessa AM, and Passos SR
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- Animals, Brazil, Dog Diseases blood, Dog Diseases diagnosis, Dogs, Ear parasitology, Leishmania donovani isolation & purification, Leishmaniasis, Visceral blood, Odds Ratio, Scapula parasitology, Dog Diseases parasitology, Leishmaniasis, Visceral diagnosis, Leishmaniasis, Visceral veterinary, Skin parasitology
- Abstract
The objective of this study was to evaluate intact skin of seroreactive dogs as a possible target for the parasitological confirmation of canine visceral leishmaniasis (CVL). For this purpose, 394 dogs identified in serological surveys carried out in the metropolitan region of Belo Horizonte were studied. Blood was collected from all animals for serology and a tissue sample was obtained from two sites for parasitological diagnosis. Skin obtained from the ear and scapular region was simultaneously analyzed in 247 animals and lesion samples and ear skin were analyzed in 147 dogs. Leishmania parasites were isolated from 310 (78.7%) animals, and all isolates were identified as Leishmania chagasi. Simultaneous isolation from two sites was possible in 240 of the 310 animals, including ear and scapular skin in 151/247 (61.1%) and ear skin and skin lesions in 89/147 (60.5%). Ours results suggest that intact skin is one of the main target sites for the parasitological confirmation of CVL in seroreactive dogs.
- Published
- 2009
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17. First report of natural infection of a bush dog (Speothos venaticus) with Leishmania (Leishmania) chagasi in Brazil.
- Author
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Figueiredo FB, Gremião ID, Pereira SA, Fedulo LP, Menezes RC, Balthazar DA, Schubach TM, and Madeira MF
- Subjects
- Animals, Brazil, Electrophoresis methods, Electrophoresis veterinary, Leishmaniasis, Visceral transmission, Male, Zoonoses parasitology, Canidae parasitology, Leishmania infantum isolation & purification, Leishmaniasis, Visceral veterinary
- Abstract
We report here the first known case of natural infection of a bush dog with Leishmania (Leishmania) chagasi in Brazil. The specimen was captured in the wild in the State of Mato Grosso and is currently being held in captivity at Fundação Jardim Zoológico, Rio de Janeiro, Brazil. The leishmaniasis was diagnosed by culture of promastigote forms in intact skin fragments and their characterization by isoenzyme electrophoresis. This report calls attention to the parasitological and etiological control of certain zoonoses, such as leishmaniasis, in wild animals kept in captivity, especially when animals are exchanged between zoos in Brazil.
- Published
- 2008
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18. Mixed infection with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in a naturally infected dog from Rio de Janeiro, Brazil.
- Author
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Madeira MF, Schubach A, Schubach TM, Pacheco RS, Oliveira FS, Pereira SA, Figueiredo FB, Baptista C, and Marzochi MC
- Subjects
- Animals, Brazil, DNA, Protozoan analysis, Disease Reservoirs, Dogs, Electrophoresis veterinary, Leishmania enzymology, Leishmania genetics, Leishmania braziliensis enzymology, Leishmania braziliensis genetics, Leishmania braziliensis isolation & purification, Leishmaniasis, Cutaneous complications, Leishmaniasis, Visceral complications, Male, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, Zoonoses, Dog Diseases parasitology, Leishmania isolation & purification, Leishmaniasis, Cutaneous veterinary, Leishmaniasis, Visceral veterinary
- Abstract
We report here the first case of co-infection with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in a naturally infected dog from Rio de Janeiro, Brazil. Isoenzyme characterisation identified the parasites isolated in culture from the cutaneous lesion as L. (V.) braziliensis and the isolates from blood and lymph node as L. (L.) chagasi. PCR analysis using specific primers followed by molecular hybridisation for direct Leishmania species identification in tissue fragments confirmed the presence of L. (V.) braziliensis DNA in the cutaneous lesion and of L. (L.) chagasi DNA in spleen and popliteal lymph node fragments. This report emphasises the importance of identification of Leishmania species infecting seropositive dogs in endemic areas, and the consequent re-assessment of control and epidemiological surveillance measures for the control of leishmaniasis, as is the case in Brazil.
- Published
- 2006
- Full Text
- View/download PDF
19. American cutaneous leishmaniasis in two cats from Rio de Janeiro, Brazil: first report of natural infection with Leishmania (Viannia) braziliensis.
- Author
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Schubach TM, Figueiredo FB, Pereira SA, Madeira MF, Santos IB, Andrade MV, Cuzzi T, Marzochi MC, and Schubach A
- Subjects
- Animals, Brazil epidemiology, Cat Diseases parasitology, Cats, Female, Leishmania braziliensis isolation & purification, Leishmaniasis, Cutaneous epidemiology, Cat Diseases epidemiology, Disease Outbreaks veterinary, Leishmaniasis, Cutaneous veterinary
- Abstract
We describe the isolation of Leishmania (Viannia) braziliensis from two female cats with American cutaneous leishmaniasis in Rio de Janeiro, Brazil. The isolates were identified as L. (V.) braziliensis by isoenzyme electrophoresis.
- Published
- 2004
- Full Text
- View/download PDF
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