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Your search keyword '"Ewing, Nadia P."' showing total 11 results
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11 results on '"Ewing, Nadia P."'

1. A Randomized Trial of Factor VIII and Neutralizing Antibodies in Hemophilia A

Author

Peyvandi, Flora, Mannucci, Pier M., Garagiola, Isabella, El-Beshlawy, Amal, Elalfy, Mohsen, Ramanan, Vijay, Eshghi, Peyman, Hanagavadi, Suresh, Varadarajan, Ramabadran, Karimi, Mehran, Manglani, Mamta V., Ross, Cecil, Young, Guy, Seth, Tulika, Apte, Shashikant, Nayak, Dinesh M., Santagostino, Elena, Mancuso, Maria Elisa, Sandoval Gonzalez, Adriana C., Mahlangu, Johnny N., Boix, Santiago Bonanad, Cerqueira, Monica, Ewing, Nadia P., Male, Christoph, Owaidah, Tarek, Arellano, Veronica Soto, Kobrinsky, Nathan L., Majumdar, Suvankar, Garrido, Rosario Perez, Sachdeva, Anupam, Simpson, Mindy, Thomas, Mathew, Zanon, Ezio, Antmen, Bulent, Kavakli, Kaan, Manco-Johnson, Marilyn J., Martinez, Monica, Marzouka, Esperanza, Mazzucconi, Maria G., Neme, Daniela, Bravo, Angeles Palomo, Aguilera, Rogelio Paredes, Prezotti, Alessandra, Schmitt, Klaus, Wicklund, Brian M., Zulfikar, Bulent, and Rosendaal, Frits R.

Published
2016
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2. Hemophilia A Inhibitor Subjects Show Unique PBMC Gene Expression Profiles That Include Up-Regulated Innate Immune Modulators.

Author

Karim, Ahmad Faisal, Soltis, Anthony R., Sukumar, Gauthaman, Königs, Christoph, Ewing, Nadia P., Dalgard, Clifton L., Wilkerson, Matthew D., and Pratt, Kathleen P.

Subjects
GENE expression profiling, IMMUNOLOGICAL tolerance, HEMOPHILIA, BLOOD cells, IMMUNE response
Abstract

Formation of pathological anti-FVIII antibodies, or "inhibitors," is the most serious complication of therapeutic FVIII infusions, affecting up to 1/3 of severe Hemophilia A (HA) patients. Inhibitor formation is a classical T-cell dependent adaptive immune response. As such, it requires help from the innate immune system. However, the roles of innate immune cells and mechanisms of inhibitor development vs. immune tolerance, achieved with or without Immune Tolerance Induction (ITI) therapy, are not well-understood. To address these questions, temporal transcriptomics profiling of FVIII-stimulated peripheral blood mononuclear cells (PBMCs) was carried out for HA subjects with and without a current or historic inhibitor using RNA-Seq. PBMCs were isolated from 40 subjects in the following groups: HA with an inhibitor that resolved either following ITI or spontaneously; HA with a current inhibitor; HA with no inhibitor history and non-HA controls. PBMCs were stimulated with 5 nM FVIII and RNA was isolated 4, 16, 24, and 48 h following stimulation. Time-series differential expression analysis was performed and distinct transcriptional signatures were identified for each group, providing clues as to cellular mechanisms leading to or accompanying their disparate anti-FVIII antibody responses. Subjects with a current inhibitor showed differential expression of 56 genes and a clustering analysis identified three major temporal profiles. Interestingly, gene ontology enrichments featured innate immune modulators, including NLRP3, TLR8, IL32, CLEC10A , and COLEC12. NLRP3 and TLR8 are associated with enhanced secretion of the pro-inflammatory cytokines IL-1β and TNFα, while IL32, which has several isoforms, has been associated with both inflammatory and regulatory immune processes. RNA-Seq results were validated by RT-qPCR, ELISAs, multiplex cytokine analysis, and flow cytometry. The inflammatory status of HA patients suffering from an ongoing inhibitor includes up-regulated innate immune modulators, which may act as ongoing danger signals that influence the responses to, and eventual outcomes of, ITI therapy. [ABSTRACT FROM AUTHOR]

Published
2020
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5. Prediction of Anti-FVIII Inhibitor Persistence By Anti-FVIII IgG Subclasses in Patients with Severe Hemophilia — A in the Sippet Cohort Study

Author

Peyvandi, Flora, Bucciarelli, Paolo, Valsecchi, Carla, Boscarino, Marco, Palla, Roberta, Santagostino, Elena, El-Beshlawy, Amal, Elalfy, Mohsen Saleh, Ramanan, Vijay M., Eshghi, Peyman, Hanagavadi, Suresh, Karimi, Mehran, Ross, Cecil, Manglani, Mamta V, Young, Guy, Seth, Tulika, Apte, Shashikant, Nayak, Dinesh M, Mancuso, Maria Elisa, Mahlangu, Johnny, Santiago, Bonanad, Cerqueira, Monica H, Ewing, Nadia P, Male, Christoph, Owaidah, Tarek, Soto Arellano, Veronica, Majumdar, Suvankar, Simpson, Mindy, Thomas, Mathew, Zanon, Ezio, Manco-Johnson, Marilyn J, Martinez, Monica, Mazzucconi, Maria Gabriella, Neme, Daniela, Paredes-Aguilera, Rogelio Alejandro, Prezotti, Alessandra N L, Schmitt, Klaus, Zulfikar, Bulent, Mannucci, Pier Mannuccio, and Rosendaal, Frits Richard

Published
2018
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6. Source of Factor VIII Replacement (PLASMATIC OR RECOMBINANT) and Incidence of Inhibitory Alloantibodies in Previously Untreated Patients with Severe Hemophilia a: The Multicenter Randomized Sippet Study

Author

Peyvandi, Flora, Mannucci, Pier Mannuccio, Garagiola, Isabella, Elalfy, Mohsen, El-Beshlawy, Amal, Ramanan, Madatha V., Eshghi, Peyman, Hanagavadi, Suresh, Varadarajan, Ramabadran, Karimi, Mehran, Manglani, Mamta V., Ross, Cecil, Young, Guy, Seth, Tulika, Apte, Shashikant, Nayak, Dinesh M., Sandoval Gonzales, Adriana C., Santagostino, Elena, Mancuso, Maria Elisa, Mahlangu, Johnny N., Bonanad, Santiago, Cerqueira, Monica, Ewing, Nadia P., Male, Christoph, Owaidah, Tarek, Soto Arellano, Veronica, Kobrinsky, Nathan L., Majumdar, Suvankar, Perez Garrido, Rosario, Sachdeva, Anupam, Simpson, Mindy, Thomas, Mathew, Zanon, Ezio, Antmen, ALI Bulent, Kavakli, Kaan, Manco-Johnson, Marilyn, Martinez, Monica, Marzouka, Esperanza, Mazzucconi, Maria Gabriella, Neme, Daniela, Palomo Bravo, Angeles, Paredes Aguilera, Rogelio, Prezotti, Alessandra Nunes Loureiro, Klaus, Schmitt, Wicklund, Brian M., Zulfikar, Bulent, and Frits, Rosendaal R.

Published
2015
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11. Issues Surrounding Genetic Screening Programs.

Author

Ewing, Nadia P.

Subjects
*LETTERS to the editor, *MEDICAL screening
Abstract

A response by Nadia Ewing on a letter to the editor about her article on genetic screening programs in the June 1981 issue of the Journal.

Published
1981

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