1. Dilatation of Golgi vesicles by monensin leads to enhanced accumulation of sugar nucleotides
- Author
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André Verbert, Roméo Cecchelli, Eliane Porchet-Hennere, René Cacan, Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Intracellular Fluid ,Glycosylation ,T-Lymphocytes ,Biophysics ,Golgi Apparatus ,Mice, Inbred Strains ,Biochemistry ,Ammonium Chloride ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,symbols.namesake ,0302 clinical medicine ,Glycosyltransferase ,Animals ,Nucleotide ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Monensin ,Furans ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,Cell permeabilisation ,Nucleoside Diphosphate Sugars ,Vesicle ,Biological Transport ,Cell Biology ,Golgi apparatus ,carbohydrates (lipids) ,Sugar nucleotide transport ,Hexosyltransferases ,chemistry ,Vacuolization ,symbols ,biology.protein ,Ammonium chloride ,030217 neurology & neurosurgery ,Intracellular - Abstract
International audience; Incubation of mouse thymocytes with 10 μM monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes whose plasma membrane has been permeabilized by ammonium chloride treatment. It is demonstrated that monensin stimulates the incorporation of labelled sialyl, fucosyl, galactosyl, and N-acetylglucosaminyl residues. This enhanced incorporation is not due to a direct effect of monensin on glycosyltransferase activities themselves but is a consequence of a higher entry and accumulation of labelled sugar nucleotides in the dilated vesicles.
- Published
- 1986