5 results on '"E, Esmer"'
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2. Holographic cell stiffness mapping using acoustic stimulation
- Author
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ESMER, GÖKHAN BORA and Varol R., Omeroglu S., Karavelioglu Z., Aydemir G., Karadag A., Meco H. E., Kocal G. C., Oruc M. E., Esmer G. B., Basbinar Y., et al.
- Subjects
ENGINEERING, ELECTRICAL & ELECTRONIC ,Elektrik ve Elektronik Mühendisliği ,Mühendislik ,Sinyal İşleme ,ENGINEERING ,Mühendislik, Bilişim ve Teknoloji (ENG) ,Fizik Bilimleri ,Signal Processing ,Physical Sciences ,MÜHENDİSLİK, ELEKTRİK VE ELEKTRONİK ,Other ,Electrical and Electronic Engineering ,Engineering, Computing & Technology (ENG) ,Diğer - Abstract
Accurate assessment of stiffness distribution is essential due to the critical role of single cell mechanobiology in the regulation of many vital cellular processes such as proliferation, adhesion, migration, and motility. Cell stiffness is one of the fundamental mechanical properties of the cell and is greatly affected by the intracellular tensional forces, cytoskeletal prestress, and cytoskeleton structure. Herein, we propose a novel holographic single-cell stiffness measurement technique that can obtain the stiffness distribution over a cell membrane at high resolution and in real-time. The proposed imaging method coupled with acoustic signals allows us to assess the cell stiffness distribution with a low error margin and label-free manner. We demonstrate the proposed technique on HCT116 (Human Colorectal Carcinoma) cells and CTC-mimicked HCT116 cells by induction with transforming growth factor-beta (TGF-\b{eta}). Validation studies of the proposed approach were carried out on certified polystyrene microbeads with known stiffness levels. Its performance was evaluated in comparison with the AFM results obtained for the relevant cells. When the experimental results were examined, the proposed methodology shows utmost performance over average cell stiffness values for HCT116, and CTC-mimicked HCT116 cells were found as 1.08 kPa, and 0.88 kPa, respectively. The results confirm that CTC-mimicked HCT116 cells lose their adhesion ability to enter the vascular circulation and metastasize. They also exhibit a softer stiffness profile compared to adherent forms of the cancer cells. Hence, the proposed technique is a significant, reliable, and faster alternative for in-vitro cell stiffness characterization tools. It can be utilized for various applications where single-cell analysis is required, such as disease modeling, drug testing, diagnostics, and many more.
- Published
- 2021
3. Effect of 900-MHz Electromagnetic Field on the Cerebellum: A Histopathological Investigation.
- Author
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Mercantepe T, Tümkaya L, Gökçe MF, Topal ZS, and Esmer E
- Abstract
Objectives: The currently widely used technological devices give rise to electromagnetic fields (EMFs) at various frequencies. Recent studies have reported that EMFs damage the central nervous system. The cerebellum is of considerable importance to human life due to its involvement in motor control, language, and cognitive-sensory functions. Damage occurring in the histological layers of the cerebellar cortex causes various neurological and psychiatric diseases, such as paralysis, tumor, autism, and schizophrenia. Our study involved a histopathological evaluation of the effects of communication systems' standard 900-MHz EMF on the cerebellum., Methods: Sprague-Dawley rats were assigned into two groups containing six animals each: control and EMF. The EMF group was exposed to a 24-h 900-MHz radiofrequency EMF over 20 days with a digital modulation signal generator installed in the middle of their cage. Ten days after EMF application, the rats were sacrificed by cervical dislocation under anesthesia induced with 50 mg/kg ketamine hydrochloride and 10 mg/kg intraperitoneal xylazine HC1., Results: Intense caspase-3 expression was seen in the Purkinje cells and granular cells exposed to a 900-MHz frequency EMF (p<0.05). Pyknotic nuclei were notable in the Purkinje and granular cells exposed to a 900-MHz EMF. We also observed a decrease in the cytoplasm of the Purkinje and granular cells. Specimens from the EMF group exhibited decreases in the thickness of the molecular cell layer, Purkinje cell layer, and granular cell layer compared with those from the control group. However, the difference was not statistically significant (p>0.05)., Conclusion: A 900-MHz EMF causes deleterious effects on the cerebellum by giving rise to apoptosis accompanied by caspase-3 expression in the Purkinje and granular cells in particular., Competing Interests: Conflict of Interest: None declared., (Copyright: © 2018 by The Medical Bulletin of Sisli Etfal Hospital.)
- Published
- 2018
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4. Frequency, severity and predictors of playing-related musculoskeletal pain in professional orchestral musicians in Germany.
- Author
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Steinmetz A, Scheffer I, Esmer E, Delank KS, and Peroz I
- Subjects
- Adult, Cross-Sectional Studies, Female, Germany epidemiology, Humans, Male, Middle Aged, Pain Measurement, Prevalence, Self Report, Severity of Illness Index, Surveys and Questionnaires, Musculoskeletal Pain epidemiology, Music, Neck Pain epidemiology, Occupational Diseases epidemiology
- Abstract
Playing-related musculoskeletal disorders (PRMD) in professional musicians are common. Existing literature demonstrates that up to 86 % of musicians are affected. The aim of the study was to evaluate the frequency of musculoskeletal pain in professional orchestral musicians with regard to their instrument affiliation. Of special interest were pain intensity and its association with predictors such as gender, instrument group, age or stage fright. Professional orchestra players completed a self-report questionnaire to assess playing-related musculoskeletal pain and its frequency and intensity in various body regions on a numeric rating scale (NRS). Relative frequencies and prevalence ratios for different instrument groups were estimated. Out of 720 approached musicians, 408 were included in the sample (response rate 57 %); overall, 89.5 % had been affected by current or past playing-related musculoskeletal pain, 62.7 % reported pain in the previous 3 months, and 8.6 % reported current pain. Pain distribution and frequency varied between instrument groups. For all instrument groups, the neck was the most common pain region. About 43 % of musicians presented more than five pain regions, in particular violin players. Approximately 40 % of musicians indicated frequent or permanent pain. Average pain intensities increased from NRS 3.8 up to a range of 5.9 and 7.4 for frequent and permanent pain, respectively. Female gender and stage fright were proven to be predictors for musculoskeletal pain. Professional orchestral musicians are greatly affected by PRMD, often experiencing frequent or permanent pain, high pain levels and pain in various body regions. As PRMD might contribute considerably to performance disability, sick leave and the possibility of premature termination of a musicians' career, this study highlights the necessity for tailored therapeutic and preventive strategies in performing arts medicine.
- Published
- 2015
- Full Text
- View/download PDF
5. The "lost lens": a new surgical technique using the Machemer lens.
- Author
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Weinstein GW, Charlton JF, and Esmer E
- Subjects
- Cataract Extraction adverse effects, Contact Lenses, Female, Humans, Intraoperative Complications surgery, Lens Subluxation etiology, Lenses, Intraocular, Male, Visual Acuity, Vitrectomy, Cataract Extraction methods, Lens Subluxation surgery
- Abstract
We describe a method of removing a posteriorly dislocated lens involving the use of a Machemer lens to visualize displaced lens remnants. Following core vitrectomy, the lens remnants are brought anteriorly and then removed by phacoemulsification. Lens implantation may then be performed with sulcus fixation. Eight of 10 patients in which this technique was used achieved 20/40 or better visual acuity.
- Published
- 1995
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