1. Whole cell solid-state NMR study of <italic>Chlamydomonas reinhardtii</italic> microalgae.
- Author
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Arnold, Alexandre A., Bourgouin, Jean-Philippe, Genard, Bertrand, Warschawski, Dror E., Tremblay, Réjean, and Marcotte, Isabelle
- Abstract
In vivo or
whole-cell solid-state NMR is an emerging field which faces tremendous challenges. In most cases, cell biochemistry does not allow the labelling of specific molecules and an in vivo study is thus hindered by the inherent difficulty of identifying, among a formidable number of resonances, those arising from a given molecule. In this work we examined the possibility of studying, by solid-state NMR, the model organismChlamydomonas reinhardtii fully and non-specifically13 C labelled. The extension of NMR-based dynamic filtering from one-dimensional to two-dimensional experiments enabled an enhanced selectivity which facilitated the assignment of cell constituents. The number of resonances detected with these robust and broadly applicable experiments appears to be surprisingly sparse. Various constituents, notably galactolipids abundant in organelle membranes, carbohydrates from the cell wall, and starch from storage grains could be unambiguously assigned. Moreover, the dominant crystal form of starch could be determined in situ. This work illustrates the feasibility and caveats of using solid-state NMR to study intact non-specifically13 C labelled micro-organisms. [ABSTRACT FROM AUTHOR]- Published
- 2018
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