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Background: Inadequate energy and nutrient intakes are a major nutritional problem in developing countries. A recent study in Beninese school-aged children in different seasons revealed a high prevalence of stunting and poor iron status that might be related to the food pattern.Objective: To analyse the food pattern and resulting energy and nutrient intakes of rural Beninese school-aged children in relation to season and school attendance.Subjects and Methods: The study was performed in northern Benin in eighty randomly selected children aged 6-8 years. Dietary intake was assessed using observed weighed records. Food, energy and nutrient intakes were measured in post- and pre-harvest seasons. Complete food consumption data sets were available for seventy-five children.Results: Food pattern showed seasonal variations. Cereals, roots and tubers were the main staple foods. Contributions of animal products to the diet were very small. The food pattern was not different for either boys v. girls or for children attending v. not attending school. Median daily energy intakes were 5.0 and 5.3 MJ in the post- and pre-harvest season, respectively. Only fat and vitamin C showed seasonal differences (P < 0.05). Energy and nutrient intakes were different for boys and girls but, unexpectedly, not for children attending v. not attending school.Conclusions: Seasonal variations in food pattern did not result in seasonality in energy and nutrient intakes. Because the children's diet was low in animal products, protein, fat and vitamin C and high in fibre, the absorption of fat, fat-soluble vitamins, carotenoids, Fe and Zn might be low. Fe and Zn bioavailability from such a diet needs further investigation. [ABSTRACT FROM AUTHOR]

Malnutrition and micronutrient deficiencies are major public health problems in developing countries. Most affected groups are children, adolescents, women of reproductive age and pregnant women. School-age children also represent an important vulnerable age category because they are still in the middle of their growth process which implies that their diets should supply appropriate macro- and micronutrients for an adequate growth and development. A better nutrition in school-age children is associated with increased school performances and a better school achievement. School-age children are dependent on their parents for access to adequate foods and nutrition but, in areas characterised by a unimodal climate with only one rainy season and one dry season, seasonal variation in food availability may also influence their nutritional status. The present study was undertaken to analyse the growth performance and iron status of school-age children in rural Benin, not only in relation to season but also to school attendance. The study was carried out in three villages in the Atacora province in northern Benin. Eighty children aged 6 to 8 years were randomly selected. Anthropometric parameters, haemoglobin level, serum ferritin and C-reactive protein were measured in the same children in the post-harvest season and the next pre-harvest season. Complete anthropometric data sets were available for 74 children while for blood analysis 69 children completed the study. In the post-harvest season, mean Z-scores for height-for-age and for weight-for-height were -1.72 ± 0.89 and -0.89 ± 0.62, respectively. The Z-score for height-for-age of children attending school (-1.55 ± 0.87) was significantly different from that of children not attending school (-2.14 ± 0.80) (P<0.05). In the post-harvest season, haemoglobin level was 119 ± 13 g/l and median serum ferritin level was 36μg/l. The haemoglobin level of children attending school (121 ± 13 g/l) was significantly different from that of children not attending school (114 ± 12 g/l) (P<0.05). The school-age children have poor growth performance and poor iron status that did not differ between seasons. The reasons behind the difference in nutritional status in relation to school attendance need further investigation. [ABSTRACT FROM AUTHOR]

The study sought to determine beliefs and practices about neglected crops in West Africa, using fonio (Digitaria exilis) as a model to understand how obstacles impede the consumption of this cereal in Bamako, the capital city of Mali. This was a crosssectional study on food ethnography in three steps: a market survey on availability of fonio, a food consumption survey on utilisation of fonio, and on beliefs on and attributes of fonio. The study covered the pre-harvest and post-harvest periods and involved key informants, food vendors, and women of reproductive age in households. Fonio, as all cereals, is available year-long on markets in Bamako, and is abundant from September to May before most of the common cereals mature. More than two-thirds (68%) of the women reported having consumed fonio one to three times a month. Fonio was more consumed as snack (djouka) on working days (62%) than on weekend and special event days, suggesting that encouraging the development of ready-to-serve fonio-based products would help increase the consumption of fonio among women in urban area. The average individual portion size of fonio was 152g/day, and the contribution to daily energy intake was 16%. A large share of the women was convinced that eating fonio was good for them (95%) and their family members (94%). Also, most of them thought that fonio had good cooking, organoleptic and nutritional qualities and could contribute to diet's variation (91% to 100%). Decision by the women to purchase or prepare fonio in the household could be favourably influenced by factors such as media, household members suffering from anaemia, neighbouring people buying fonio and shortage of other cereals; whereas shortage of fonio products (77%), high cost of fonio products (69%), difficult cooking process (51%), and lack of knowledge about processing and cooking fonio (43%) were likely to limit fonio consumption among the women. Also, in the present study, fonio was perceived to be for rich people by more than half (58%) of the women. Improving cooking process and knowledge of the women about fonio cooking, as well as creating a demand for the women with the household's head and others through media, social and health care services would help increase fonio consumption in Bamako. [ABSTRACT FROM AUTHOR]

Background: To our knowledge, the impact of a nutrition education program combined with an increase in bioavailable dietary iron to treat iron-deficiency anemia has never been studied in adolescent girls.Objective: To evaluate the impact of an intensive dietary program for the treatment of iron-deficiency anemia in 34 intervention and 34 control boarding-school girls aged 12 to 17 years from Benin.Methods: A quasi-experimental design consisting of 4 weeks of nutrition education combined with an increase in the content and bioavailability of dietary iron for 22 weeks was implemented in the intervention school, but not in the control school. Data were obtained from both groups from a nutrition knowledge questionnaire, 24-hour dietary recalls, anthropometric measurements, measurement of iron status indices, and screening for malarial and intestinal parasitic infections.Results: Nutrition knowledge scores and mean intakes of nutrients, including dietary iron, absorbable iron, and vitamin C, were significantly higher in the intervention group (p < .05) than in the control group after 26 weeks. Mean hemoglobin and serum ferritin values were also significantly higher in the intervention group than in the control group (122 vs. 112 g/L [p = .0002] and 32 vs. 19 microg/L [p = .04], respectively), whereas the prevalence of anemia (32% vs. 85% [p = .005] and iron-deficiency anemia (26% vs. 56% [p = .04]) was significantly lower in the intervention group than in the control group. No significant differences between the groups were observed in intestinal parasitic infections or malaria status postintervention.Conclusions: A multidietary strategy aiming to improve available dietary iron can reduce iron-deficiency anemia in adolescent girls. [ABSTRACT FROM AUTHOR]

In the developing world, food intake of young children is often insufficient for growth. Reduced appetite due to several factors including micronutrient deficiencies might be an explanation. We hypothesized that a multivitamin–multimineral supplementation will improve appetite of stunted children in south of Benin. Multivitamin–multimineral supplements (VITALIA-tablets) contain 11 vitamins and 8 minerals. Stunted children (Ht/Age Z score<−2) of 17–32 months old were randomly assigned to multivitamin–multimineral (n=48) or placebo (n=53) group. Supplementation was daily and supervised throughout 6 weeks. Knee-heel length, length, weight, arm circumference and appetite were assessed once a week for the three weeks preceding and the three weeks following the six-week intervention period. Growth was additionally assessed 4 months after intervention. Each appetite test day, morbidity data and mother''s report on child''s appetite throughout the preceding day were recorded. Reported appetite, intake of test food and knee-heel length increased after supplementation in both groups (p<0·05), but were not different between groups. Growth was similar 4 months after the intervention. Morbidity was comparable in both groups before as well as after supplementation. We conclude that 6-week multivitamin–multimineral supplementation alone failed to improve appetite and growth of stunted young children. [Copyright &y& Elsevier]

Appetite measurements were performed in 109 Beninese children aged 18–30 months to develop a tool for appetite evaluation in young children in nutritional intervention programmes. Two test foods were identified as appropriate for these children: a maize porridge (aklui) and rice (riz-au-gras). Ad libitum intakes of the foods served after an overnight fast according to a standardized offering procedure were measured on 3 days. The children''s habitual intakes were measured during 3 consecutive days not overlapping with the days when the test meals were provided. Energy intake from the test foods was comparable to breakfast energy intake which was 0·8–1·0 MJ, representing 21% of total daily energy intake. Energy intake from aklui was significantly correlated with daily intake (r=0·41,n =38, p<0·05) and with energy intake from breakfast (r=0·52,p <0·01). Correlations concerning riz-au-gras were less pronounced and non-significant. Reproducibility (as coefficient of variation) of the appetite test as calculated from the triplicate measurements was 40% for aklui and 25% for riz-au-gras. This reproducibility is better than that of the habitual breakfast intake (43–45%). In conclusion, the appetite test used in our studies can be considered as an appropriate tool in appetite evaluations. [Copyright &y& Elsevier]

In developing countries, low food intake is often reported in children < 5 y old. Reduced appetite may be a contributing factor. We investigated whether a combination of a multivitamin-multimineral supplement and additional iron treatment improved appetite and growth of 18- to 30-mo-old stunted and anemic Beninese children. The study was placebo-controlled using VITALIA tablets (11 vitamins and 10 minerals) and ferrous fumarate tablets (66 mg of iron). One hundred fifty stunted (height-for-age Z score < -2) and anemic children (hemoglobin < 110 g/L) were randomly assigned to one of four groups: group 1, multivitamin-multimineral plus iron; group 2, multivitamin-multimineral plus placebo; group 3, placebo plus placebo; and group 4, placebo plus iron. Supplementation was daily and supervised for 6 wk. Appetite, knee-heel length, dietary intakes and morbidity were assessed before and after supplementation. Length, weight, arm circumference and hemoglobin concentration were assessed before, just after supplementation and 4 mo after the intervention. Appetite was assessed by means of an appetite test using a test food, riz-au-gras, eaten ad libitum after an overnight fast. Dietary intakes were assessed during three consecutive days in a subsample by means of the observed weighed record method. Energy intake from the habitual breakfast was significantly correlated with that from the test food (r = 0.49, n = 38, P = 0.002). There were no differences among groups in changes in appetite and growth performance. The habitual diet of the children was monotonous and contained only small amounts of animal products. The morbidity status of the children was comparable in all study groups, before as well as after supplementation. We conclude that the 6-wk multivitamin-multimineral supplementation with additional iron treatment failed to improve the appetite and growth of the children. [ABSTRACT FROM AUTHOR]

Presents a study which assessed the effects of iron and deworming on linear growth performance of preschoolers. Methods; Results; Discussion.

The genus Pantoea forms a complex of more than 25 species, among which several cause diseases of various crop plants, including rice. Notably, strains of Pantoea ananatis and P. stewartii have been repeatedly reported to cause bacterial leaf blight of rice, whereas other authors have observed that P. agglomerans can also cause bacterial leaf blight of rice. The contribution of these and perhaps other species of Pantoea to plant diseases and yield losses of crop plants is currently not well documented, partly due to the lack of efficient diagnostic tools. Using 32 whole-genome sequences of the three major plant-pathogenic Pantoea spp., a set of PCR primers that detect each of the three species P. agglomerans , P. ananatis , and P. stewartii was designed. A multiplex PCR scheme which can distinguish these three species and also detects members of other Pantoea spp. was further developed. Upon validation on a set of reference strains, 607 suspected Pantoea strains that were isolated from rice leaves or seed originating from 11 African countries were screened. In total, 41 P. agglomerans strains from 8 countries, 79 P. ananatis strains from 9 countries, 269 P. stewartii strains from 9 countries, and 218 unresolved Pantoea strains from 10 countries were identified. The PCR protocol allowed detection of Pantoea bacteria grown in vitro, in planta, and in rice seed. The detection threshold was estimated as total genomic DNA at 0.5 ng/µl and heated cells at 1 × 10 4 CFU/ml. This new molecular diagnostic tool will help to accurately diagnose major plant-pathogenic species of Pantoea . Due to its robustness, specificity, sensitivity, and cost efficiency, it will be very useful for plant protection services and for the epidemiological surveillance of these important crop-threatening bacteria.

Background: Cognitive impairment is recognized as a common symptom experienced by cancer survivors which impacts on quality of life (QoL) and day-to-day activities. One of the treatment options is the use of psychostimulants but the evidence supporting its use remains unclear., Objectives: To identify the level of evidence of psychostimulants' effect on the management of cognitive impairment in adult cancer survivors., Methods: Electronic databases (MEDLINE, EMBASE, CENTRAL, CINAHL) and reference lists of relevant reviews were searched from inception to December 2017, with no language restrictions applied. Randomized controlled trials (RCTs), evaluating the effect of psychostimulants on cognitive impairment among cancer patients with no primary or secondary brain tumor or brain radiation, were included. The primary outcome was cognitive function changes, whereas secondary outcomes were adverse events (AEs) and QoL., Results: Six RCTs were included: three studies investigating methylphenidate and three modafinil, with a total of 244 and 146 patients, respectively. Due to important differences in methodologies between studies, a meta-analysis was assumed inappropriate for the primary outcome. A narrative synthesis was performed. One study using methylphenidate and two using modafinil demonstrated improvements in some cognitive functions as measured by objective cognitive assessment tests. Psychostimulants did not improve QoL and were not associated with more AEs., Conclusion: To date, limited evidence is available to estimate the usefulness (or lack) of psychostimulants on cognitive function in this population.

The present study investigated the association between weight status and Fe deficiency (ID) among urban Malian women of reproductive age. Height, weight, serum ferritin (SF), soluble transferrin receptor (sTfR) and C-reactive protein (CRP) concentrations were measured in sixty apparently healthy women aged 15-49 years old in Bamako, Mali. Prevalences of overweight and obese were 19 and 9 %, respectively. SF was non-significantly different between overweight (84 μg/l) and normal-weight women (52 μg/l). The prevalence of ID (SF < 12 μg/l) was 9 % in the overweight group and no true ID (sTfR>8·3 mg/l) cases were recorded in the overweight and obese groups. The prevalence OR of ID (SF < 12 μg/l) in the overweight group was NS (OR = 0·3; P = 0·363). Conversely, the chronic energy deficiency group was at a significantly higher risk of ID than the normal-weight group, adjusting or not for CRP (OR = 7·7; 95 % CI 1·49, 39·96; P = 0·015). The lack of association between overweight and ID in the present study could be due to the fact that the excess of body fat of the women might not be critical to induce chronic inflammation related to reduced Fe absorption. Future research based on a larger convenience sample should be designed to further investigate associations between overweight, obesity and ID in developing countries.

Background: Iron deficiency anemia (IDA) affects many young women in sub-Saharan Africa. Its etiology is multifactorial, but the major cause is low dietary iron bioavailability exacerbated by parasitic infections such as malaria., Objective: We investigated whether asymptomatic Plasmodium falciparum parasitemia in Beninese women would impair absorption of dietary iron or utilization of circulating iron., Design: Iron absorption and utilization from an iron-fortified sorghum-based meal were estimated by using oral and intravenous isotope labels in 23 afebrile women with a positive malaria smear (asexual P. falciparum parasitemia; > 500 parasites/μL blood). The women were studied while infected, treated, and then restudied 10 d after treatment. Iron status, hepcidin, and inflammation indexes were measured before and after treatment., Results: Treatment reduced low-grade inflammation, as reflected by decreases in serum ferritin, C-reactive protein, interleukin-6, interleukin-8, and interleukin-10 (P < 0.05); this was accompanied by a reduction in median serum hepcidin of ≈ 50%, from 2.7 to 1.4 nmol/L (P < 0.005). Treatment decreased serum erythropoietin and growth differentiation factor 15 (P < 0.05). Clearance of parasitemia increased geometric mean dietary iron absorption (from 10.2% to 17.6%; P = 0.008) but did not affect systemic iron utilization (85.0% compared with 83.1%; NS)., Conclusions: Dietary iron absorption is reduced by ≈ 40% in asymptomatic P. falciparum parasitemia, likely because of low-grade inflammation and its modulation of circulating hepcidin. Because asymptomatic parasitemia has a protracted course and is very common in malarial areas, this effect may contribute to IDA and blunt the efficacy of iron supplementation and fortification programs. This trial was registered at clinicaltrials.gov as NCT01108939.

The prevalence of micronutrient deficiency is high among women of reproductive age living in urban Mali. Despite this, there are little data on the dietary intake of micronutrients among women of reproductive age in Mali. This research tested the relationship between the quantity of intake of 21 possible food groups and estimated usual micronutrient (folate, vitamin B-12, calcium, riboflavin, niacin, vitamin A, iron, thiamin, vitamin B-6, vitamin C, and zinc) intakes and a composite measure of adequacy of 11 micronutrients [mean probability of adequacy (MPA)] based on the individual probability of adequacy (PA) for the 11 micronutrients. Food group and micronutrient intakes were calculated from 24-h recall data in an urban sample of Malian women. PA was lowest for folate, vitamin B-12, calcium, and riboflavin. The overall MPA for the composite measure of 11 micronutrients was 0.47 ± 0.18. Grams of intake from the nuts/seeds, milk/yogurt, vitamin A-rich dark green leafy vegetables (DGLV), and vitamin C-rich vegetables food groups were correlated (Spearman's rho = 0.20-0.36; P < 0.05) with MPA. Women in the highest consumption groups of nuts/seeds and DGLV had 5- and 6-fold greater odds of an MPA > 0.5, respectively. These findings can be used to further the development of indicators of dietary diversity and to improve micronutrient intakes of women of reproductive age.

Unlabelled: To our knowledge, the impact of socioeconomic and health related factors on the iron status of adolescent girls has never been studied in Benin., Objective: These factors were studied in 180 girls aged 12 to 17 years living in two boarding schools from South Benin., Methods: Iron deficiency (ID) was defined as either serum ferritin (SF) < 20 microg/L or SF between 20-50 microg/L plus two abnormal values in the three following parameters: serum iron < 11 micromol/L, total iron binding capacity > 73 micromol/L or transferrin saturation < 20%. Socioeconomic and health related factors were obtained from each participant by mean of an interview using a standardized and pre-tested questionnaire., Results: Almost half the participants reported some health problems in the last four months before the study, whereas more than 75% auto-medicated before going to the hospital. The majority of the girls believed themselves to be in good health, although only 16% could define the term 'anemia'. Multivariate regression analysis indicated that girls whose mother was a manual worker (P = .002), who came from a larger family (P = .0001), and who auto-medicated (P = .014), had a lower hemoglobin level, whereas girls who had started their menstruation (P = .008) had a lower SF level. In a logistic regression analysis, girls from a large family size and whose mother was a manual worker showed a higher risk of IDA (OR = 3.5; 95% CI = 1.1-2.5; P = .04; OR = 3.0; 95% CI = 1.2-2.2; P = .04 respectively)., Conclusion: The findings indicate that iron deficiency is related to the occupation of the mother, family size, auto-medication, and menstruation.

A 26-week nutrition intervention, including 4 weeks of nutrition education, combined with an increase in the content and bioavailability of dietary iron for 22 weeks was carried out in 34 intervention and 34 control adolescent girls suffering from mild iron deficiency anemia (IDA). In post-intervention, hemoglobin and serum ferritin were significantly higher in the intervention group, whereas the incidence of IDA was significantly lower in the intervention group compared to the control group. Nutrition knowledge scores were significantly higher in intervention girls compared to control girls. Dietary changes to improve available dietary iron can reduce iron deficiency anemia.

Iron deficiency (ID) is the most prevalent micronutrient deficiency in the world, particularly in developing countries. Blood samples and a qualitative FFQ on Fe- and vitamin C-rich foods were obtained in 180 adolescent girls aged 12 to 17 years living in two boarding schools from south Benin. ID, defined as serum ferritin either 73 micromol/l or transferrin saturation<20%, was found in 32% of subjects. Anaemia (Hb<120 g/l) was found in 51% of adolescents, while 24% suffered from iron-deficiency anaemia (IDA) (ID and Hb<20 g/l). After adjusting for confounding factors (age, mother's and father's occupation, household size) in a logistic regression equation, subjects having a low meat consumption (beef, mutton, pork) (<4 times/week) were more than twice as likely to suffer from ID (OR=2.43; 95% CI 1.72, 3.35; P=0.04). Adolescents consuming less fruits (<4 times/week) also had a higher likelihood of suffering from ID (OR=1.53; 95% CI 1.31, 2.80; P=0.03). Finally, subjects whose meat consumption was low were twice as likely to suffer from IDA (OR=2.24; 95% CI 1.01, 4.96; P=0.04). The prevalence of ID represents an important health problem in these Beninese adolescent girls. A higher consumption of Fe-rich foods and of promoters of Fe absorption (meat factor and vitamin C) is recommended to prevent ID deficiency in these subjects.

Intestinal parasitic infections (IPI), especially helminths, represent a major public health problem that increase iron deficiency anaemia in developing countries. This study investigated the prevalence, risk factors and nutritional consequences of IPIs in 180 adolescent girls aged 12-17 years living in two boarding schools in southern Benin. Data were collected using a structured questionnaire and laboratory analysis of blood and faecal samples. The relationships between socioeconomic indicators, IPIs and iron status were analysed using logistic regression analysis. Fifty percent of the subjects were infected with at least one IPI: 2% with helminths, 41% with protozoa and 7% with two or more intestinal parasites. Adolescent girls from a large family and those whose mothers were manual workers showed a higher risk of intestinal parasitism (odds ratio (OR)=3.5, 95% CI 2.5-5.2 (P=0.02) and OR=2.4, 95% CI 2.0-3.0 (P=0.03), respectively). Likewise, drinking untreated water was also a high risk factor for infection (OR=2.3, 95% CI 1.5-2.4; P=0.03). No significant association was observed between IPIs and iron deficiency or iron deficiency anaemia, which can be explained by the low wormload observed. These findings reinforce the need to involve mothers in health initiatives to control intestinal parasitism in Benin.