105 results on '"Dono M"'
Search Results
2. Evaluating mismatch repair deficiency in colorectal cancer biopsy specimens
- Author
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Grillo, F., Paudice, M., Gambella, A., Bozzano, S., Sciallero, S., Puccini, A., Lastraioli, S., Dono, M., Parente, P., Vanoli, A., Angerilli, V., Fassan, M., and Mastracci, L.
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- 2023
- Full Text
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3. The storm of NGS in NSCLC diagnostic-therapeutic pathway: How to sun the real clinical practice
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De Maglio G., Pasello G., Dono M., Fiorentino M., Follador A., Sciortino M., Malapelle U., Tiseo M., De Maglio, G., Pasello, G., Dono, M., Fiorentino, M., Follador, A., Sciortino, M., Malapelle, U., Tiseo, M., De Maglio G., Pasello G., Dono M., Fiorentino M., Follador A., Sciortino M., Malapelle U., and Tiseo M.
- Subjects
Lung Neoplasms ,NGS ,Carcinoma, Non-Small-Cell Lung ,MTB ,Mutation ,Humans ,High-Throughput Nucleotide Sequencing ,Precision Medicine ,NSCLC ,Human - Abstract
The increasing number of approved drugs along with next generation sequencing (NGS) technologies look out as potential revolution of biomolecular characterization of non-small-cell lung cancer (NSCLC). Nevertheless, several aspects impact on success rate of NGS in clinical practice: a multidisciplinary approach and thorough knowledge of strengths and limits of each technologic diagnostic tool are required. Crucial preliminary step is the selection of the best available sample before testing, aware of clinical condition and setting of disease. Genomic data should be than integrated in the clinical context and matched with available therapeutic options; Molecular Tumor Boards (MTB) are worldwide emerging interdisciplinary groups implemented to transfer the impact of precision medicine in clinical practice. In order to guarantee equity in treatment, these considerations should find their application widely and rapidly. Aim of this review is offering an overview of emerging biomarkers, relative upcoming targeted drugs, and new diagnostic chances with an authors’ perspective about a real-life diagnostic-therapeutic algorithm useful for daily clinical practice.
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- 2022
4. 83P Circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) in eary high risk breast cancer: The CITUCEL trial update
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Borea, R., Cardinali, B., Arecco, L., De Luca, G., Garuti, A., Mazzitelli, C., Sciutto, A., Poggio, F., Conte, B., Buzzatti, G., Blondeaux, E., Carli, F., Anselmi, G., Profumo, A., Coronel-Vargas, G.F., Ballestrero, A., Ferrando, L., Zoppoli, G., Dono, M., and Del Mastro, L.
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- 2024
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5. ItaLynch: an ongoing Italian study to evaluate the feasibility of mainstreaming the diagnosis of Lynch syndrome in colorectal cancer patients
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Puccini, A., Grillo, F., Fassan, M., Lonardi, S., Genuardi, M., Cannizzaro, R., Cavestro, G.M., Marmorino, F., Conca, V., Salvatore, L., Bergamo, F., Tosi, F., Morano, F., Daprà, V., Molica, C., Barana, D., Guglielmi, A., Signorelli, C., D’Amico, M., Zoratto, F., Iacono, D., Morabito, A., Martini, G., Fabbroncini, A., Duro, M., Bruera, G., Auriemma, A., Bonanni, B., Percesepe, A., Dono, M., Battistuzzi, L., Labianca, R., Boni, L., and Sciallero, S.
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- 2024
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6. P-205 Streamlining the diagnostic pathway for Lynch syndrome in colorectal cancer patients: A ten-year experience in a single Italian center
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Puccini, A., Nardin, S., Trevisan, L., Lastraioli, S., Ricciotti, I., Damiani, A., Bregni, G., Pastorino, A., Martelli, V., Murialdo, R., Gismondi, V., Battistuzzi, L., Varesco, L., Dono, M., Grillo, F., and Sciallero, S.
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- 2023
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7. 1099P Front-line liquid biopsy for early molecular assessment and treatment of hospitalized lung cancer patients
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Parisi, F., De Luca, G., Rossi, G., Coco, S., Dellepiane, C., Bennicelli, E., Zinoli, L., Zullo, L., Alama, A., Mosconi, M., Mora, M., Ballestrero, A., Montecucco, F., Marconi, S., Pronzato, P., Del Mastro, L., Nozza, P., Bellodi, A., Dono, M., and Genova, C.
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- 2022
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8. 597P Endometrial carcinoma and mismatch repair deficiency: Clinical association and universal screening for Lynch syndrome
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Puglisi, S., Ponzano, M., Perachino, M., Pirrone, C., Damassi, A., Bregni, G., Puccini, A., Grassi, M., Trevisan, L., Gismondi, V., Dono, M., Lastraioli, S., Fedele, P., Cremante, M., Gandini, A., Giannelli, F., Mammoliti, S., Vellone, V.G., Sciallero, M.S., and Iaia, M.L.
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- 2022
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9. Large expert-curated database for benchmarking document similarity detection in biomedical literature search
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Brown, P. Zhou, Y. Tan, A.-C. El-Esawi, M.A. Liehr, T. Blanck, O. Gladue, D.P. Almeida, G.M.F. Cernava, T. Sorzano, C.O. Yeung, A.W.K. Engel, M.S. Chandrasekaran, A.R. Muth, T. Staege, M.S. Daulatabad, S.V. Widera, D. Zhang, J. Meule, A. Honjo, K. Pourret, O. Yin, C.-C. Zhang, Z. Cascella, M. Flegel, W.A. Goodyear, C.S. van Raaij, M.J. Bukowy-Bieryllo, Z. Campana, L.G. Kurniawan, N.A. Lalaouna, D. Hüttner, F.J. Ammerman, B.A. Ehret, F. Cobine, P.A. Tan, E.-C. Han, H. Xia, W. McCrum, C. Dings, R.P.M. Marinello, F. Nilsson, H. Nixon, B. Voskarides, K. Yang, L. Costa, V.D. Bengtsson-Palme, J. Bradshaw, W. Grimm, D.G. Kumar, N. Martis, E. Prieto, D. Sabnis, S.C. Amer, S.E.D.R. Liew, A.W.C. Perco, P. Rahimi, F. Riva, G. Zhang, C. Devkota, H.P. Ogami, K. Basharat, Z. Fierz, W. Siebers, R. Tan, K.H. Boehme, K.A. Brenneisen, P. Brown, J.A.L. Dalrymple, B.P. Harvey, D.J. Ng, G. Werten, S. Bleackley, M. Dai, Z. Dhariwal, R. Gelfer, Y. Hartmann, M.D. Miotla, P. Tamaian, R. Govender, P. Gurney-Champion, O.J. Kauppila, J.H. Zhang, X. Echeverría, N. Subhash, S. Sallmon, H. Tofani, M. Bae, T. Bosch, O. Cuív, P.O. Danchin, A. Diouf, B. Eerola, T. Evangelou, E. Filipp, F. Klump, H. Kurgan, L. Smith, S.S. Terrier, O. Tuttle, N. Ascher, D.B. Janga, S.C. Schulte, L.N. Becker, D. Browngardt, C. Bush, S.J. Gaullier, G. Ide, K. Meseko, C. Werner, G.D.A. Zaucha, J. Al-Farha, A.A. Greenwald, N.F. Popoola, S.I. Rahman, S. Xu, J. Yang, S.Y. Hiroi, N. Alper, O.M. Baker, C.I. Bitzer, M. Chacko, G. Debrabant, B. Dixon, R. Forano, E. Gilliham, M. Kelly, S. Klempnauer, K.-H. Lidbury, B.A. Lin, M.Z. Lynch, I. Ma, W. Maibach, E.W. Mather, D.E. Nandakumar, K.S. Ohgami, R.S. Parchi, P. Tressoldi, P. Xue, Y. Armitage, C. Barraud, P. Chatzitheochari, S. Coelho, L.P. Diao, J. Doxey, A.C. Gobet, A. Hu, P. Kaiser, S. Mitchell, K.M. Salama, M.F. Shabalin, I.G. Song, H. Stevanovic, D. Yadollahpour, A. Zeng, E. Zinke, K. Alimba, C.G. Beyene, T.J. Cao, Z. Chan, S.S. Gatchell, M. Kleppe, A. Piotrowski, M. Torga, G. Woldesemayat, A.A. Cosacak, M.I. Haston, S. Ross, S.A. Williams, R. Wong, A. Abramowitz, M.K. Effiong, A. Lee, S. Abid, M.B. Agarabi, C. Alaux, C. Albrecht, D.R. Atkins, G.J. Beck, C.R. Bonvin, A.M.J.J. Bourke, E. Brand, T. Braun, R.J. Bull, J.A. Cardoso, P. Carter, D. Delahay, R.M. Ducommun, B. Duijf, P.H.G. Epp, T. Eskelinen, E.-L. Fallah, M. Farber, D.B. Fernandez-Triana, J. Feyerabend, F. Florio, T. Friebe, M. Furuta, S. Gabrielsen, M. Gruber, J. Grybos, M. Han, Q. Heinrich, M. Helanterä, H. Huber, M. Jeltsch, A. Jiang, F. Josse, C. Jurman, G. Kamiya, H. de Keersmaecker, K. Kristiansson, E. de Leeuw, F.-E. Li, J. Liang, S. Lopez-Escamez, J.A. Lopez-Ruiz, F.J. Marchbank, K.J. Marschalek, R. Martín, C.S. Miele, A.E. Montagutelli, X. Morcillo, E. Nicoletti, R. Niehof, M. O'Toole, R. Ohtomo, T. Oster, H. Palma, J.-A. Paterson, R. Peifer, M. Portilla, M. Portillo, M.C. Pritchard, A.L. Pusch, S. Raghava, G.P.S. Roberts, N.J. Ross, K. Schuele, B. Sergeant, K. Shen, J. Stella, A. Sukocheva, O. Uversky, V.N. Vanneste, S. Villet, M.H. Viveiros, M. Vorholt, J.A. Weinstock, C. Yamato, M. Zabetakis, I. Zhao, X. Ziegler, A. Aizat, W.M. Atlas, L. Bridges, K.M. Chakraborty, S. Deschodt, M. Domingues, H.S. Esfahlani, S.S. Falk, S. Guisado, J.L. Kane, N.C. Kueberuwa, G. Lau, C.L. Liang, D. Liu, E. Luu, A.M. Ma, C. Ma, L. Moyer, R. Norris, A.D. Panthee, S. Parsons, J.R. Peng, Y. Pinto, I.M. Reschke, C.R. Sillanpää, E. Stewart, C.J. Uhle, F. Yang, H. Zhou, K. Zhu, S. Ashry, M. Bergsland, N. Berthold, M. Chen, C.-E. Colella, V. Cuypers, M. Eskew, E.A. Fan, X. Gajda, M. Gonzálezlez-Prendes, R. Goodin, A. Graham, E.B. Groen, E.J.N. Gutiérrez-Sacristán, A. Habes, M. Heffler, E. Higginbottom, D.B. Janzen, T. Jayaraman, J. Jibb, L.A. Jongen, S. Kinyanjui, T. Koleva-Kolarova, R.G. Li, Z. Liu, Y.-P. Lund, B.A. Lussier, A.A. Ma, L. Mier, P. Moore, M.D. Nagler, K. Orme, M.W. Pearson, J.A. Prajapati, A.S. Saito, Y. Tröder, S.E. Uchendu, F. Verloh, N. Voutchkova, D.D. Abu-Zaid, A. Bakkach, J. Baumert, P. Dono, M. Hanson, J. Herbelet, S. Hobbs, E. Kulkarni, A. Kumar, N. Liu, S. Loft, N.D. Reddan, T. Senghore, T. Vindin, H. Xu, H. Bannon, R. Chen, B. Cheung, J.T.K. Cooper, J. Esnakula, A.K. Feghali, K.A. Ghelardi, E. Gnasso, A. Horbar, J. Lai, H.M. Li, J. Ma, L. Ma, R. Pan, Z. Peres, M.A. Pranata, R. Seow, E. Sydes, M. Testoni, I. Westermair, A.L. Yang, Y. Afnan, M. Albiol, J. Albuquerque, L.G. Amir, S. Amiya, E. Amorim, R.M. An, Q. Andersen, S.U. Aplin, J.D. Argyropoulos, C. Asmann, Y.W. Assaeed, A.M. Atanasov, A.G. Atchison, D.A. Avery, S.V. Avillach, P. Baade, P.D. Backman, L. Badie, C. Baldi, A. Ball, E. Bardot, O. Barnett, A.G. Basner, M. Batra, J. Bazanova, O.M. Beale, A. Beddoe, T. Bell, M.L. Berezikov, E. Berners-Price, S. Bernhardt, P. Berry, E. Bessa, T.B. Billington, C. Birch, J. Blakely, R.D. Blaskovich, M.A.T. Blum, R. Boelaert, M. Bogdanos, D. Bosch, C. Bourgoin, T. Bouvard, D. Boykin, L.M. Bradley, G. Braun, D. Brownlie, J. Brühl, A. Burt, A. Butler, L.M. Byrareddy, S.N. Byrne, H.J. Cabantous, S. Calatayud, S. Candal, E. Carlson, K. Casillas, S. Castelvetro, V. Caswell, P.T. Cavalli, G. Cerovsky, V. Chagoyen, M. Chen, C.-S. Chen, D.F. Chen, H. Chen, H. Chen, J.-T. Chen, Y. Cheng, C. Cheng, J. Chinapaw, M. Chinopoulos, C. Cho, W.C.S. Chong, L. Chowdhury, D. Chwalibog, A. Ciresi, A. Cockcroft, S. Conesa, A. Cook, P.A. Cooper, D.N. Coqueret, O. Corea, E.M. Costa, A. Costa, E. Coupland, C. Crawford, S.Y. Cruz, A.D. Cui, H. Cui, Q. Culver, D.C. D'Angiulli, A. Dahms, T.E.S. Daigle, F. Dalgleish, R. Danielsen, H.E. Darras, S. Davidson, S.M. Day, D.A. Degirmenci, V. Demaison, L. Devriendt, K. Ding, J. Dogan, Y. Dong, X.C. Donner, C.F. Dressick, W. Drevon, C.A. Duan, H. Ducho, C. Dumaz, N. Dwarakanath, B.S. Ebell, M.H. Eisenhardt, S. Elkum, N. Engel, N. Erickson, T.B. Fairhead, M. Faville, M.J. Fejzo, M.S. Festa, F. Feteira, A. Flood-Page, P. Forsayeth, J. Fox, S.A. Franks, S.J. Frentiu, F.D. Frilander, M.J. Fu, X. Fujita, S. Galea, I. Galluzzi, L. Gani, F. Ganpule, A.P. García-Alix, A. Gedye, K. Giordano, M. Giunta, C. Gleeson, P.A. Goarant, C. Gong, H. Gora, D. Gough, M.J. Goyal, R. Graham, K.E. Grande-Pérez, A. Graves, P.M. Greidanus, H. Grice, D. Grunau, C. Gumulya, Y. Guo, Y. Gurevich, V.V. Gusev, O. Hacker, E. Hage, S.R. Hagen, G. Hahn, S. Haller, D.M. Hammerschmidt, S. Han, J. Han, R. Handfield, M. Hapuarachchi, H.C. Harder, T. Hardingham, J.E. Heck, M. Heers, M. Hew, K.F. Higuchi, Y. Hilaire, C.St. Hilton, R. Hodzic, E. Hone, A. Hongoh, Y. Hu, G. Huber, H.P. Hueso, L.E. Huirne, J. Hurt, L. Idborg, H. Ikeo, K. Ingley, E. Jakeman, P.M. Jensen, A. Jia, H. Jia, H. Jia, S. Jiang, J. Jiang, X. Jin, Y. Jo, D. Johnson, A.M. Johnston, M. Jonscher, K.R. Jorens, P.G. Jorgensen, J.O.L. Joubert, J.W. Jung, S.-H. Junior, A.M. Kahan, T. Kamboj, S.K. Kang, Y.-K. Karamanos, Y. Karp, N.A. Kelly, R. Kenna, R. Kennedy, J. Kersten, B. Khalaf, R.A. Khalid, J.M. Khatlani, T. Khider, T. Kijanka, G.S. King, S.R.B. Kluz, T. Knox, P. Kobayashi, T. Koch, K.-W. Kohonen-Corish, M.R.J. Kong, X. Konkle-Parker, D. Korpela, K.M. Kostrikis, L.G. Kraiczy, P. Kratz, H. Krause, G. Krebsbach, P.H. Kristensen, S.R. Kumari, P. Kunimatsu, A. Kurdak, H. Kwon, Y.D. Lachat, C. Lagisz, M. Laky, B. Lammerding, J. Lange, M. Larrosa, M. Laslett, A.L. Laverman, G.D. Leclair, E.E. Lee, K.-W. Lee, M.-Y. Lee, M.-S. Li, G. Li, J. Lieb, K. Lim, Y.Y. Lindsey, M.L. Line, P.-D. Liu, D. Liu, F. Liu, H. Liu, H. Lloyd, V.K. Lo, T.-W. Locci, E. Loidl, J. Lorenzen, J. Lorkowski, S. Lovell, N.H. Lu, H. Lu, W. Lu, Z. Luengo, G.S. Lundh, L.-G. Lysy, P.A. Mabb, A. Mack, H.G. Mackey, D.A. Mahdavi, S.R. Maher, P. Maher, T. Maity, S.N. Malgrange, B. Mamoulakis, C. Mangoni, A.A. Manke, T. Manstead, A.S.R. Mantalaris, A. Marsal, J. Marschall, H.-U. Martin, F.L. Martinez-Raga, J. Martinez-Salas, E. Mathieu, D. Matsui, Y. Maza, E. McCutcheon, J.E. McKay, G.J. McMillan, B. McMillan, N. Meads, C. Medina, L. Merrick, B.A. Metzger, D.W. Meunier, F.A. Michaelis, M. Micheau, O. Mihara, H. Mintz, E.M. Mizukami, T. Moalic, Y. Mohapatra, D.P. Monteiro, A. Montes, M. Moran, J.V. Morozov, S.Y. Mort, M. Murai, N. Murphy, D.J. Murphy, S.K. Murray, S.A. Naganawa, S. Nammi, S. Nasios, G. Natoli, R.M. Nguyen, F. Nicol, C. van Nieuwerburgh, F. Nilsen, E.B. Nobile, C.J. O'Mahony, M. Ohlsson, S. Olatunbosun, O. Olofsson, P. Ortiz, A. Ostrikov, K. Otto, S. Outeiro, T.F. Ouyang, S. Paganoni, S. Page, A. Palm, C. Paradies, Y. Parsons, M.H. Parsons, N. Pascal, P. Paul, E. Peckham, M. Pedemonte, N. Pellizzon, M.A. Petrelli, M. Pichugin, A. Pinto, C.J.C. Plevris, J.N. Pollesello, P. Polz, M. Ponti, G. Porcelli, P. Prince, M. Quinn, G.P. Quinn, T.J. Ramula, S. Rappsilber, J. Rehfeldt, F. Reiling, J.H. Remacle, C. Rezaei, M. Riddick, E.W. Ritter, U. Roach, N.W. Roberts, D.D. Robles, G. Rodrigues, T. Rodriguez, C. Roislien, J. Roobol, M.J. Rowe, A. Ruepp, A. van Ruitenbeek, J. Rust, P. Saad, S. Sack, G.H. Santos, M. Saudemont, A. Sava, G. Schrading, S. Schramm, A. Schreiber, M. Schuler, S. Schymkowitz, J. Sczyrba, A. Seib, K.L. Shi, H.-P. Shimada, T. Shin, J.-S. Shortt, C. Silveyra, P. Skinner, D. Small, I. Smeets, P.A.M. So, P.-W. Solano, F. Sonenshine, D.E. Song, J. Southall, T. Speakman, J.R. Srinivasan, M.V. Stabile, L.P. Stasiak, A. Steadman, K.J. Stein, N. Stephens, A.W. Stewart, D.I. Stine, K. Storlazzi, C. Stoynova, N.V. Strzalka, W. Suarez, O.M. Sultana, T. Sumant, A.V. Summers, M.J. Sun, G. Tacon, P. Tanaka, K. Tang, H. Tanino, Y. Targett-Adams, P. Tayebi, M. Tayyem, R. Tebbe, C.C. Telfer, E.E. Tempel, W. Teodorczyk-Injeyan, J.A. Thijs, G. Thorne, S. Thrift, A.G. Tiffon, C. Tinnefeld, P. Tjahjono, D.H. Tolle, F. Toth, E. Del Tredici, A.L. Tsapas, A. Tsirigotis, K. Turak, A. Tzotzos, G. Udo, E.E. Utsumi, T. Vaidyanathan, S. Vaillant, M. Valsesia, A. Vandenbroucke, R.E. Veiga, F.H. Vendrell, M. Vesk, P.A. Vickers, P. Victor, V.M. Villemur, R. Vohl, M.-C. Voolstra, C.R. Vuillemin, A. Wakelin, S. Waldron, L. Walsh, L.J. Wang, A.Y. Wang, F. Wang, Y. Watanabe, Y. Weigert, A. Wen, J.-C. Wham, C. White, E.P. Wiener, J. Wilharm, G. Wilkinson, S. Willmann, R. Wilson, C. Wirth, B. Wojan, T.R. Wolff, M. Wong, B.M. Wu, T.-W. Wuerbel, H. Xiao, X. Xu, D. Xu, J.W. Xu, J. Xue, B. Yalcin, S. Yan, H. Yang, E.-C. Yang, S. Yang, W. Ye, Y. Ye, Z.-Q. Yli-Kauhaluoma, J. Yoneyama, H. Yu, Y. Yuan, G.-C. Yuh, C.-H. Zaccolo, M. Zeng, C. Zevnik, B. Zhang, C. Zhang, L. Zhang, Y. Zhang, Y. Zhang, Z. Zhang, Z.-Y. Zhao, Y. Zhou, M. Zuberbier, T. Aanei, C.M. Ahmad, R. Al-Lawama, M. Alanio, A. Allardyce, J. Alonso-Caneiro, D. Atack, J.M. Baier, D. Bansal, A. Benezeth, Y. Berbesque, C. Berrevoet, F. Biedermann, P.H.W. Bijleveld, E. Bittner, F. Blombach, F. van den Bos, W. Boudreau, S.A. Bramoweth, A.D. Braubach, O. Cai, Y. Campbell, M. Cao, Z. Catry, T. Chen, X. Cheng, S. Chung, H.-J. Chávez-Fumagalli, M.A. Conway, A. Costa, B.M. Cyr, N. Dean, L.T. Denzel, M.S. Dlamini, S.V. Dudley, K.J. Dufies, M. Ecke, T. Eckweiler, D. Eixarch, E. El-Adawy, H. Emmrich, J.V. Eustace, A.J. Falter-Wagner, C.M. Farhoudi, R. Fuss, J. Gao, J. Gill, M.R. Gloyn, L. Goggs, R. Govinden, U. Greene, G. Greiff, V. Grundle, D.S. Grüneberg, P. Gumede, N. Haore, G. Harrison, P. Hoenner, X. Hojsgaard, D. Hori, H. Ikonomopoulou, M.P. Jeurissen, P. Johnson, D.M. Kabra, D. Kamagata, K. Karmakar, C. Kasian, O. Kaye, L.K. Khan, M.M. Kim, Y.-M. Kish, J.K. Kobold, S. Kohanbash, G. Kohls, G. Kugler, J.-M. Kumar, G. Lacy-Colson, J. Latif, A. Lauschke, V.M. Li, B. Lim, C.J. Liu, F. Liu, X. Lu, J.-J. Lu, Q. Mahavadi, P. Marzocchi, U. McGarrigle, C.A. van Meerten, T. Min, R. Moal, I. Molari, M. Molleman, L. Mondal, S.R. van de Mortel, T. Moss, W.N. Moultos, O.A. Mukherjee, M. Nakayama, K. Narayan, E. Navaratnarajah Neumann, P.-A. Nie, J. Nie, Y. Niemeyer, F. Nolan, F. Nwaiwu, O. Oldenmenger, W.H. Olumayede, E. Ou, J. Pallebage-Gamarallage, M. Pearce, S.P. Pelkonen, T. Pelleri, M.C. Pereira, J.L. Pheko, M. Pinto, K.A. Piovesan, A. Pluess, M. Podolsky, I.M. Prescott, J. Qi, D. Qi, X. Raikou, V.D. Ranft, A. Rhodes, J. Rotge, J.-Y. Rowe, A.D. Saggar, M. Schuon, R.A. Shahid, S. Shalchyan, V. Shirvalkar, P. Shiryayev, O. Singh, J. Smout, M.J. Soares, A. Song, C. Srivastava, K. Srivastava, R.K. Sun, J. Szabo, A. Szymanski, W. Tai, C.N.P. Takeuchi, H. Tanadini-Lang, S. Tang, F. Tao, W. Theron, G. Tian, C.F. Tian, Y.-S. Tuttle, L.M. Valenti, A. Verlot, P. Walker, M. Wang, J. Welter, D. Winslade, M. Wu, D. Wu, Y.-R. Xiao, H. Xu, B. Xu, J. Xu, Z. Yang, D. Yang, M. Yankilevich, P. You, Y. Yu, C. Zhan, J. Zhang, G. Zhang, K. Zhang, T. Zhang, Y. Zhao, G. Zhao, J. Zhou, X. Zhu, Z. Ajani, P.A. Anazodo, U.C. Bagloee, S.A. Bail, K. Bar, I. Bathelt, J. Benkeser, D. Bernier, M.L. Blanchard, A.M. Boakye, D.W. Bonatsos, V. Boon, M.H. Bouboulis, G. Bromfield, E. Brown, J. Bul, K.C.M. Burton, K.J. Butkowski, E.G. Carroll, G. Chao, F. Charrier, E.E. Chen, X. Chen, Y.-C. Chenguang Choi, J.R. Christoffersen, T. Comel, J.C. Cosse, C. Cui, Y. van Dessel, P. Dhaval Diodato, D. Duffey, M. Dutt, A. Egea, L.G. El-Said, M. Faye, M. Fernandez-Fernandez, B. Foley, K.G. Founou, L.L. Fu, F. Gadelkareem, R.A. Galimov, E. Garip, G. Gemmill, A. Gouil, Q. Grey, J. Gridneva, Z. Grothe, M.J. Grébert, T. Guerrero, F. Guignard, L. Haenssgen, M.J. Hasler, D. Holgate, J.Y. Huang, A. Hulse-Kemp, A.M. Jean-Quartier, C. Jeon, S.-M. Jia, Y. Jutzeler, C. Kalatzis, P. Karim, M. Karsay, K. Keitel, A. Kempe, A. Keown, J.R. Khoo, C.M. Khwaja, N. Kievit, R.A. Kosanic, S. Koutoukidis, D.A. Kramer, P. Kumar, D. Kiraǧ, N. Lanza, G. Le, T.D. Leem, J.W. Leightley, D. Leite, A. Lercher, L. Li, Y. Lim, R. Lima, L.R.A. Lin, L. Ling, T. Liu, Y. Liu, Z. Lu, Y. Lum, F.M. Luo, H. Machhi, J. Macleod, A. Macwan, I. Madala, H.R. Madani, N. de Maio, N. Makowiecki, K. Mallinson, D.J. Margelyte, R. Maria, C. Markonis, Y. Marsili, L. Mavoa, S. McWilliams, L. Megersa, M. Souto-Maior, C. Menichetti, J. Mercieca-Bebber, R. Miller, J.J. Minde, D.-P.M. Minges, A. Mishra, E. Mishra, V.R. Moores, C. Morrice, N. Moskalensky, A.E. Navarin, N. Negera, E. Nolet, P. Nordberg, A. Nordén, R. Nowicki, J.P. Olova, N. Olszewski, P. Onzima, R. Pan, C.-L. Park, C. Park, D.I. Park, S. Patil, C.D. Pedro, S.A. Perry, S.R. Peter, J. Peterson, B.M. Pezzuolo, A. Pozdnyakov, I. Qian, S. Qin, L. Rafe, A. Raote, I. Raza, A. Rebl, H. Refai, O. Regan, T. Richa, T. Richardson, M.F. Robinson, K.R. Rossoni, L. Rouet, R. Safaei, S. Schneeberger, P.H.H. Schwotzer, D. Sebastian, A. Selinski, J. Seltmann, S. Sha, F. Shalev, N. Shang, J.-L. Singer, J. Singh, M. Smith, T. Solomon-Moore, E. Song, L. Soraggi, S. Stanley, R. Steckhan, N. Strobl, F. Subissi, L. Supriyanto, I. Surve, C.R. Suzuki, T. Syme, C. Sörelius, K. Tang, Y. Tantawy, M. Tennakoon, S. Teseo, S. Toelzer, C. Tomov, N. Tovar, M. Tran, L. Tripathi, S. Tuladhar, A.M. Ukubuiwe, A.C. Ung, C.O.L. Valgepea, K. Vatanparast, H. Vidal, A. Wang, F. Wang, Q. Watari, R. Webster, R. Webster, R. Wei, J. Wibowo, D. Wingenbach, T.S.H. Xavier, R.M. Xiao, S. Xiong, P. Xu, S. Xu, S. Yao, R. Yao, W. Yin, Q. Yu, Y. Zaitsu, M. Zeineb, Z. Zhan, X.-Y. Zhang, J. Zhang, R. Zhang, W. Zhang, X. Zheng, S. Zhou, B. Zhou, X. Ahmad, H. Akinwumi, S.A. Albery, G.F. Alhowimel, A. Ali, J. Alshehri, M. Alsuhaibani, M. Anikin, A. Azubuike, S.O. Bach-Mortensen, A. Baltiansky, L. Bartas, M. Belachew, K.Y. Bhardwaj, V. Binder, K. Bland, N.S. Boah, M. Bullen, B. Calabrò, G.E. Callahan, T.J. Cao, B. Chalmers, K. Chang, W. Che, Z. Chen, A.T.Y. Chen, H. Chen, H. Chen, Y. Chen, Z. Choi, Y. Chowdhury, M.A.K. Christensen, M.R. Cooke, R.S.C. Cottini, M. Covington, N.V. Cunningham, C. Delarocque, J. Devos, L. Dhar, A.R. Ding, K.-F. Dong, K. Dong, Z. Dreyer, N. Ekstrand, C. Fardet, T. Feleke, B.E. Feurer, T. Freitas, A. Gao, T. Gebremedhin Giganti, F. Grabowski, P. Guerra-Mora, J.R. Guo, C. Guo, X. Gupta, H. He, S. Heijne, M. Heinemann, S. Hogrebe, A. Huang, Z. Iskander-Rizk, S. Iyer, L.M. Jahan, Y. James, A.S. Joel, E. Joffroy, B. Jégousse, C. Kambondo, G. Karnati, P. Kaya, C. Ke, A. Kelly, D. Kickert, R. Kidibule, P.E. Kieselmann, J.P. Kim, H.J. Kitazawa, T. Lamberts, A. Li, Y. Liang, H. Linn, S.N. Litfin, T. Liusuo, W. Lygirou, V. Mahato, A.K. Mai, Z.-M. Major, R.W. Mali, S. Mallis, P. Mao, W. Marvin-Dowle, K. Mason, L.D. Merideth, B. Merino-Plaza, M.J. Merlaen, B. Messina, R. Mishra, A.K. Muhammad, J. Musinguzi, C. Nanou, A. Naqash, A. Nguyen, J.T. Nguyen, T.T.H. Ni, D. Nida Notcovich, S. Ohst, B. Ollivier, Q.R. Osses, D.F. Peng, X. Plantinga, A. Pulia, M. Rafiq, M. Raman, A. Raucher-Chéné Rawski, R. Ray, A. Razak, L.A. Rudolf, K. Rusch, P. Sadoine, M.L. Schmidt, A. Schurr, R. Searles, S. Sharma, S. Sheehan, B. Shi, C. Shohayeb, B. Sommerlad, A. Strehlow, J. Sun, X. Sundar, R. Taherzadeh, G. Tahir, N.D.M. Tang, J. Testa, J. Tian, Z. Tingting, Q. Verheijen, G.P. Vickstrom, C. Wang, T. Wang, X. Wang, Z. Wei, P. Wilson, A. Wyart Yassine, A.-A. Yousefzadeh, A. Zare, A. Zeng, Z. Zhang, C. Zhang, H. Zhang, L. Zhang, T. Zhang, W. Zhang, Z. Zhou, J. Zhu, D. Adamo, V. Adeyemo, A.A. Aggelidou, M. Al-Owaifeer, A.M. Al-Riyami, A.Z. Alzghari, S.K. Andersen, V. Angus, K. Asaduzzaman, M. Asady, H. Ato, D. Bai, X. Baines, R.L. Ballantyne, M. Ban, B. Beck, J. Ben-Nafa, W. Black, E. Blancher, A. Blankstein, R. Bodagh, N. Borges, P. Brooks, A. Brox-Ponce, J. Brunetti, A. Canham, C.D. Carninci, P. Carvajal, R. Chang, S.C. Chao, J. Chatterjee, P. Chen, H. Chen, L. Chen, Y.-C. Chhatriwalla, A.K. Chikowe, I. Chuang, T.-J. Collevatti, R.G. Cornejo, D.A.V. Cuenda, A. Dao, M. Dauga, D. Deng, Z. Devkota, K. Doan, L.V. Elewa, Y.H.A. Fan, D. Faruk, M. Feifei, S. Ferguson, T.S. Fleres, F. Foster, E.J. Foster, S. Furer, T. Gao, Y. Garcia-Rivera, E.J. Gazdar, A. George, R.B. Ghosh, S. Gianchecchi, E. Gleason, J.M. Hackshaw, A. Hall, A. Hall, R. Harper, P. Hogg, W.E. Huang, G. Hunter, K.E. Ijzerman, A.P. Jesus, C. Jian, G. Lewis, J.S., Jr. Kanj, S.S. Kaur, H. Kelly, S. Kheir, F. Kichatova, V.S. Kiyani, M. Klein, R. Kovesi, T. Kraschnewski, J.L. Kumar, A.P. Labutin, D. Lazo-Langner, A. Leclercq, G. Li, M. Li, Q. Li, T. Li, Y. Liao, W.-T. Liao, Z.-Y. Lin, J. Lizer, J. Lobreglio, G. Lowies, C. Lu, C. Majeed, H. Martin, A. Martinez-Sobrido, L. Meresh, E. Middelveen, M. Mohebbi, A. Mota, J. Mozaheb, Z. Muyaya, L. Nandhakumar, A. Ng, S.H.X. Obeidat, M. Oh, D.-H. Owais, M. Pace-Asciak, P. Panwar, A. Park, C. Patterson, C. Penagos-Tabaree, F. Pianosi, P.T. Pinzi, V. Pridans, C. Psaroulaki, A. Pujala, R.K. Pulido-Arjona, L. Qi, P.-F. Rahman, P. Rai, N.K. Rassaf, T. Refardt, J. Ricciardi, W. Riess, O. Rovas, A. Sacks, F.M. Saleh, S. Sampson, C. Schmutz, A. Sepanski, R. Sharma, N. Singh, M. Spearman, P. Subramaniapillai, M. Swali, R. Tan, C.M. Tellechea, J.I. Thomas, L.-M. Tong, X. Vavvas, D.G. Veys, R. Vitriol, V. Wang, H.-D. Wang, J. Wang, J. Waugh, J. Webb, S.A. Williams, B.A. Workman, A.D. Xiang, T. Xie, L.-X. Xu, J. Xu, T. Yang, C. Yoon, J.G. Yuan, C.M. Zaritsky, A. Zhang, Y. Zhao, H. Zuckerman, H. Lyu, R. Pullan, W. RELISH Consortium
- Abstract
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical science. © The Author(s) 2019. Published by Oxford University Press.
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- 2019
10. 1276P Deep molecular characterization of never smoker non-small cell lung cancer (NSCLC) patients
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Dellepiane, C., De Luca, G., Tagliamento, M., Coco, S., Rossi, G., Bello, M.G. Dal, Mora, M., Zullo, L., Alama, A., Bottini, A., Sacco, G., Cella, E., Bennicelli, E., Borea, R., Murianni, V., Parisi, F., Salvi, S., Pronzato, P., Dono, M., and Genova, C.
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- 2021
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11. Subepithelial B cells in the human palatine tonsil. I. Morphologic, cytochemical and phenotypic characterization of a novel subset
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DONO M., BURGIO L., TACCHETTI, CARLO, FAVRE A., ZUPO S., TABORELLI G., CHIORAZZI N., GROSSI C. E., FERRARINI M., Dono, M., Burgio, L., Tacchetti, Carlo, Favre, A., Zupo, S., Taborelli, G., Chiorazzi, N., Grossi, C. E., and Ferrarini, M.
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- 1996
12. B lymphocytes in humans express ZAP-70 when activated in vivo
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Cutrona, G, Colombo, M, Matis, S, Reverberi, D, Dono, M, Tarantino, V, Chiorazzi, N, and Ferrarini, Manlio
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- 2006
13. CD 10 is a marker for cycling cells with propensity to apoptosis in childhood ALL
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Cutrona, G., Tasso, P., Dono, M., Roncella, S., Ulivi, M., Carpaneto, E. M., Fontana, V., Comis, M., Morabito, F., Spinelli, M., Frascella, E., Boffa, L. C., Basso, G., Pistoia, V., and Ferrarini, Manlio
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- 2002
14. Heterogeneity of tonsilar subepithelial B lymphocytes, the splenic marginal zone equivalents
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Dono, M., Zupo, S., Leanza, N., Melioli, G., Fogli, M., Melagrana, A., Chiorazzi, N., and Ferrarini, Manlio
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- 2000
15. Accumulation of clonally-related B lymphocytes in the cerebrospinal fluid of multiple sclerosis patients
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Colombo, M., Dono, M., Gazzola, P., Roncella, S., Valetto, A., Chiorazzi, N., Mancardi, GIOVANNI LUIGI, and Ferrarini, Manlio
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- 2000
16. Examples of in vivo isotype switching in IgM+ chronic lymphocytic leukemia B cells
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Fais, Franco, Sellars, B., Ghiotto, FABIO GIUSEPPE, Yan, X. J., Dono, M., Allen, S. L., Budman, D., Dittmar, K., Kolitz, J., Lichtman, S. M., Schulman, P., Schuster, M., Vinciguerra, V. P., Rai, K., Stevenson, F. K., Gregersen, P. K., Ferrarini, Manlio, and Chiorazzi, N.
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- 1996
17. CD38 EXPRESSION DISTINGUISHES TWO GROUPS OF B CELL-CHRONIC LYMPHOCYTIC LEUKEMIAS WITH DIFFERENT RESPONSE TO ANTI IgM ANTIBODIES AND PROPENSITY TO APOPTOSIS
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Zupo, S., Isnardi, L., Megna, M., Massara, M., Malavasi, F., Dono, M., Cosulich, E., and Ferrarini, Manlio
- Published
- 1996
18. Somatic diversification and selection of ig h and L chain variable region genes in IgG+ CD5+ chronic lymphocytic leukemia B cells
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Hashimoto, S, Dono, M, Wakai, M, Allen, Sl, Lichtman, Sm, Schulman, P, Vinciguerra, Vp, Sellars, B, Gregersen, Pk, Ferrarini, Manlio, and Chiorazzi, N.
- Published
- 1995
19. Mutation frequencies of GNAQ, GNA11, BAP1, SF3B1, EIF1AX and TERT in uveal melanoma: detection of an activating mutation in the TERT gene promoter in a single case of uveal melanoma.
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Dono, M, Angelini, G, Cecconi, M, Amaro, A, Esposito, A I, Mirisola, V, Maric, I, Lanza, F, Nasciuti, F, Viaggi, S, Gualco, M, Bandelloni, R, Truini, M, Coviello, D A, Zupo, S, Mosci, C, and Pfeffer, U
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- *
MELANOMA , *OCULAR tumors , *TELOMERASE reverse transcriptase , *DNA microarrays , *METASTASIS - Abstract
Background:Uveal melanoma is the most frequent primary tumour of the eye. It is molecularly clearly distinct from cutaneous melanoma and shows a different pattern of driver mutations. The influence of sunlight ultraviolet (UV) exposure on the aetiology of uveal melanoma is a matter of debate. The recent identification of driver mutations in the promoter of the telomerase reverse transcriptase (TERT) gene with UV-induced cytidine-to-thymidine transitions in cutaneous melanoma prompted us to investigate whether these mutations also occur in uveal melanoma.Methods:We analysed 50 cases of uveal melanoma obtained from enucleation surgery for mutations in the genes GNAQ, GNA11, BAP1, SF3B1, EIFAX1 and TERT, measured gene expression using microarrays and analysed gene copy numbers by SNP arrays.Results:We detected a TERT mutation in only one case of a 57-year-old white male patient with clinical and histopathological features typical for uveal melanoma. The tumour showed mutations in GNA11 and EIF1AX that are typical for uveal melanoma and absent from cutaneous melanoma. No mutations were detected in GNAQ, BAP1 and SF3B1 that are frequently mutated in uveal melanoma. Both copies of chromosome 3 were retained. Several tumours among which the one carrying the TERT promoter mutation showed elevated TERT expression. Consistent with previous reports, GNAQ is inversely associated with chromosome 3 monosomy and metastasis. BAP1 mutations are significantly associated with chromosome 3 monosomy but not with relapse.Conclusion:These data indicate that TERT mutations are rare in uveal melanoma. No conclusion can be drawn on their potential influence on tumour progression. [ABSTRACT FROM AUTHOR]
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- 2014
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20. CD10 is a marker for cycling cells with propensity to apoptosis in childhood ALL.
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Cutrona, G., Tasso, P., Dono, M., Roncella, S., Ulivi, M., Carpaneto, E.M., Fontana, V., Comis, M., Morabito, F., Spinelli, M., Frascella, E., Boffa, L.C., Basso, G., Pistoia, V., and Ferrarini, M.
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LYMPHOBLASTIC leukemia in children ,APOPTOSIS ,CELL proliferation - Abstract
CD10 constitutes a favourable prognostic marker for childhood acute lymphoblastic leukaemia. Since correlations between CD10, cell cycle and apoptotic abilities were demonstrated in various cell types, we investigated whether differences existed in the cycling/apoptotic abilities of CD10-positive and CD10-negative B acute lymphoblastic leukaemia cells. Twenty-eight cases of childhood acute lymphoblastic leukaemia (mean age of 6.8 years) were subdivided into two groups according to high (17 cases, 93.2+/-4.5%, MRFI 211+/-82 CD10-positive cells) or low (11 cases, 11.5+/-6.2%, MRFI 10+/-7 CD10-negative cells) expression of CD10. CD10-positive acute lymphoblastic leukaemia cells were cycling cells with elevated c-myc levels and propensity to apoptosis, whereas CD10-negative acute lymphoblastic leukaemia cells had lower cycling capacities and c-myc levels, and were resistant to apoptosis in vitro. A close correlation between all these properties was demonstrated by the observations that the few CD10-positive cells found in the CD10-negative acute lymphoblastic leukaemia group displayed elevated c-myc and cycling capacities and were apoptosis prone. Moreover, exposure of CD10-positive acute lymphoblastic leukaemia B cells to a peptide nucleic acid anti-gene specific for the second exon of c-myc caused inhibition of c-myc expression and reduced cell cycling and apoptotic abilities as well as decreased CD10 expression. [ABSTRACT FROM AUTHOR]
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- 2002
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21. Possible Role of Cytokines in the Pathogenesis of Non-Organ Specific Autoimmunity.
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Zupo, S., Dono, M., Azzoni, L., Chiorazzi, N., and Ferrarini, M.
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- 1992
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22. Report from the OECI Oncology Days 2014.
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van Harten, W. H., Stanta, G., Bussolati, G., Riegman, P., Hoefler, G., Becker, K. F., Folprecht, G., Truini, M., Haybaeck, J., Buiga, R., Dono, M., Bagg, A., López Guerrero, J. A., Zupo, S., Lemare, F., de Lorenzo, F., Goedbloed, N., Razavi, D., Lövey, J., and Cadariu, P. A.
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ONCOLOGY , *CANCER treatment , *MEDICAL care costs , *CONFERENCES & conventions - Abstract
The 2014 OECI Oncology Days was held at the 'Prof. Dr. Ion Chiricuta' Oncology Institute in Cluj, Romania, from 12 to 13 June. The focus of this year's gathering was on developments in personalised medicine and other treatment advances which have made the cost of cancer care too high for many regions throughout Europe. [ABSTRACT FROM AUTHOR]
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- 2015
- Full Text
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23. Large expert-curated database for benchmarking document similarity detection in biomedical literature search
- Author
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Zeineb, Zian, Brown, Peter, Tan, Aik-Choon, El-Esawi, Mohamed A., Liehr, Thomas, Blanck, Oliver, Gladue, Douglas P., Almeida, Gabriel M. F., Cernava, Tomislav, Sorzano, Carlos O., Yeung, Andy W. K., Engel, Michael S., Chandrasekaran, Arun Richard, Muth, Thilo, Staege, Martin S., Daulatabad, Swapna V., Widera, Darius, Zhang, Junpeng, Meule, Adrian, Honjo, Ken, Pourret, Olivier, Yin, Cong-Cong, Zhang, Zhongheng, Cascella, Marco, Flegel, Willy A., Goodyear, Carl S., Raaij, Mark J. van, Bukowy-Bieryllo, Zuzanna, Campana, Luca G., Kurniawan, Nicholas A., Lalaouna, David, Hüttner, Felix J., Ammerman, Brooke A., Ehret, Felix, Cobine, Paul A., Tan, Ene-Choo, Han, Hyemin, Xia, Wenfeng, McCrum, Christopher, Dings, Ruud P. M., Marinello, Francesco, Nilsson, Henrik, Nixon, Brett, Voskarides, Konstantinos, Yang, Long, Costa, Vincent D., Bengtsson-Palme, Johan, Bradshaw, William, Grimm, Dominik G., Kumar, Nitin, Martis, Elvis, Prieto, Daniel, Sabnis, Sandeep C., Amer, Said E. D. R., Liew, Alan W. C., Perco, Paul, Rahimi, Farid, Riva, Giuseppe, Zhang, Chongxing, Devkota, Hari P., Ogami, Koichi, Basharat, Zarrin, Fierz, Walter, Siebers, Robert, Tan, Kok-Hian, Boehme, Karen A., Brenneisen, Peter, Brown, James A. L., Dalrymple, Brian P., Harvey, David J., Ng, Grace, Werten, Sebastiaan, Bleackley, Mark, Dai, Zhanwu, Dhariwal, Raman, Gelfer, Yael, Hartmann, Marcus D., Miotla, Pawel, Tamaian, Radu, Govender, Pragashnie, Gurney-Champion, Oliver J., Kauppila, Joonas H., Zhang, Xiaolei, Echeverría, Natalia, Subhash, Santhilal, Sallmon, Hannes, Tofani, Marco, Bae, Taeok, Bosch, Oliver, Cuív, Páraic O., Danchin, Antoine, Diouf, Barthelemy, Eerola, Tuomas, Evangelou, Evangelos, Filipp, Fabian V., Klump, Hannes, Kurgan, Lukasz, Smith, Simon S., Terrier, Olivier, Tuttle, Neil, Ascher, David B., Janga, Sarath C., Schulte, Leon N., Becker, Daniel, Browngardt, Christopher, Bush, Stephen J., Gaullier, Guillaume, Ide, Kazuki, Meseko, Clement, Werner, Gijsbert D. A., Zaucha, Jan, Al-Farha, Abd A., Greenwald, Noah F., Popoola, Segun I., Rahman, Md Shaifur, Xu, Jialin, Yang, Sunny Y., Hiroi, Noboru, Alper, Ozgul M., Baker, Chris I., Bitzer, Michael, Chacko, George, Debrabant, Birgit, Dixon, Ray, Forano, Evelyne, Gilliham, Matthew, Kelly, Sarah, Klempnauer, Karl-Heinz, Lidbury, Brett A., Lin, Michael Z., Lynch, Iseult, Ma, Wujun, Maibach, Edward W., Mather, Diane E., Nandakumar, Kutty S., Ohgami, Robert S., Parchi, Piero, Tressoldi, Patrizio, Xue, Yu, Armitage, Charles, Barraud, Pierre, Chatzitheochari, Stella, Coelho, Luis P., Diao, Jiajie, Doxey, Andrew C., Hu, Pingzhao, Kaiser, Stefan, Mitchell, Kate M., Salama, Mohamed F., Shabalin, Ivan G., Song, Haijun, Stevanovic, Dejan, Yadollahpour, Ali, Zeng, Erliang, Zinke, Katharina, Alimba, C. 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H., Xavier, Rose M., Xiao, Shumin, Xiong, Peng, Xu, Shicai, Xu, Shilin, Yao, Ruifeng, Yao, Wen, Yin, Qinan, Yu, Yongbo, Zaitsu, Masayoshi, Zhan, Xiao-Yong, Zhang, Jilei, Zhang, Rongqiang, Zhang, Wei, Zhang, Xianglilan, Zheng, Shan, Zhou, Bailing, Zhou, Xiaoyan, Ahmad, Haroon, Akinwumi, Sayo A., Albery, Gregory F., Alhowimel, Ahmed, Ali, Junaid, Alshehri, Mansour, Alsuhaibani, Mohammed, Anikin, Andrey, Azubuike, Samuel O., Bach-Mortensen, Anders, Baltiansky, Lior, Bartas, Martin, Belachew, Kiflemariam Y., Bhardwaj, Vivek, Binder, Karin, Bland, Nicholas S., Boah, Michael, Bullen, Benjamin, Calabrò, Giovanna E., Callahan, Tiffany J., Cao, Bing, Chalmers, Kelsey, Chang, Wei, Che, Zhengping, Chen, Andrew T. Y., Chen, Haimin, Chen, Huaming, Chen, Youning, Chen, Zhao, Choi, YoungRok, Chowdhury, Mohiuddin A. K., Christensen, Martin R., Cooke, Robert S. 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M., Tang, Jun, Testa, Jean, Tian, Zhiqi, Tingting, Qian, Verheijen, Geert P., Vickstrom, Casey, Wang, Teng, Wang, Xiaomin, Wang, Zhenxing, Wei, Pan, Wilson, Alex, Wyart, Yassine, Abdul-Amir, Yousefzadeh, Abbas, Zare, Asma, Zeng, Zhen, Zhang, Chengrong, Zhang, Haowen, Zhang, Linxing, Zhang, Tongchuan, Zhang, Weijia, Zhang, Zhe, Zhou, Jianyu, Zhu, Dongjie, Adamo, Vincenzo, Adeyemo, Adebolajo A., Aggelidou, Maria, Al-Owaifeer, Adi M., Al-Riyami, Arwa Z., Alzghari, Saeed K., Andersen, Vibeke, Angus, Kathryn, Asaduzzaman, Muhammad, Asady, Hadi, Ato, Dai, Bai, Xiaoyong, Baines, Rebecca L., Ballantyne, Maghan, Ban, Bo, Beck, Jill, Ben-Nafa, Walid, Black, Emma, Blancher, Antoine, Blankstein, Ron, Bodagh, Neil, Borges, Paulo A. 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S., Kiyani, Musa, Klein, Reinhild, Kovesi, Tom, Kraschnewski, Jennifer L., Kumar, Addanki P., Labutin, Dmitry, Lazo-Langner, Alejandro, Leclercq, Guy, Li, Maoteng, Li, Qingchun, Li, Tangliang, Li, Yongzhe, Liao, Wei-Ting, Liao, Zheng-yin, Lin, Jessica, Lizer, J., Lobreglio, Giambattista, Lowies, Cher, Lu, Cheng, Majeed, Haroon, Martin, Adam, Martinez-Sobrido, Luis, Meresh, Edwin, Middelveen, Marianne, Mohebbi, Alireza, Mota, Jorge, Mozaheb, Zahra, Muyaya, Ley, Nandhakumar, Amar, Ng, Sheryl H. X., Obeidat, Monther, Oh, Deog-Hwan, Owais, Mohammed, Pace-Asciak, Pia, Panwar, Ajay, Park, Caroline, Patterson, Chris, Penagos-Tabaree, Felipe, Pianosi, Paolo T., Pinzi, Valentina, Pridans, Clare, Psaroulaki, Anna, Pujala, Ravi Kumar, Pulido-Arjona, Leonardo, Qi, Peng-Fei, Rahman, Proton, Rai, Nayanjot K., Rassaf, Tienush, Refardt, Julie, Ricciardi, Walter, Riess, Olaf, Rovas, Alexandros, Sacks, Frank M., Saleh, Sherif, Sampson, Christopher, Schmutz, Axel, Sepanski, Robert, Sharma, Neeraj, Singh, Manisha, Spearman, Paul, Subramaniapillai, Mehala, Swali, Ritu, Tan, Cher M., Tellechea, Juan I., Thomas, Lisa-Marie, Tong, Xin, Vavvas, Demetrios G., Veys, Ralf, Vitriol, Veronica, Wang, Horng-Dar, Wang, Jinhui, Wang, Jiucun, Waugh, Jason, Webb, S. A., Williams, Brendan A., Workman, Alan D., Xiang, Tingxiu, Xie, Li-Xin, Xu, Jun, Xu, Taosheng, Yang, Chongjun, Yoon, Jihoon G., Yuan, Christina M., Zaritsky, Arno, Zhang, Yao, Zhao, Haochen, Zuckerman, Hannah, Lyu, Ran, Pullan, Wayne, Zhou, Yaoqi, Gobet, Angélique, Sadoine, Margaux L., Ontwikkelingspsychologie (Psychologie, FMG), British Lung Foundation, Brown, P, Zhou, Y, Tan, A, El-Esawi, M, Liehr, T, Blanck, O, Gladue, D, Almeida, G, Cernava, T, Sorzano, C, Yeung, A, Engel, M, Chandrasekaran, A, Muth, T, Staege, M, Daulatabad, S, Widera, D, Zhang, J, Meule, A, Honjo, K, Pourret, O, Yin, C, Zhang, Z, Cascella, M, Flegel, W, Goodyear, C, van Raaij, M, Bukowy-Bieryllo, Z, Campana, L, Kurniawan, N, Lalaouna, D, Huttner, F, Ammerman, B, Ehret, F, Cobine, P, Tan, E, Han, H, Xia, W, Mccrum, C, Dings, R, Marinello, F, Nilsson, H, Nixon, B, Voskarides, K, Yang, L, Costa, V, Bengtsson-Palme, J, Bradshaw, W, Grimm, D, Kumar, N, Martis, E, Prieto, D, Sabnis, S, Amer, S, Liew, A, Perco, P, 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Univ Calif Irvine, Univ Hosp Leuven, Chongqing Med Univ, Childrens Hosp Kings Daughters, China Three Gorges Univ, and Xiangtan Univ
- Subjects
Technology and Engineering ,SCIENTIFIC SEARCH ,Expert-curated database ,Biokemia, solu- ja molekyylibiologia - Biochemistry, cell and molecular biology ,Databases ,RElevant LIterature SearcH consortium ,Medicine and Health Sciences ,Biomedical research ,benchmarking ,Biology ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,Settore MED/42 - IGIENE GENERALE E APPLICATA ,database ,Computer. Automation ,Science & Technology ,0804 Data Format ,relisch ,Scientific research in health sciences ,Mathematics and Statistics ,litearture search ,relisch , database ,biomedical research ,Biomedical literature ,Original Article ,RELISH ,Mathematical & Computational Biology ,RECOMMENDER-SYSTEMS ,Life Sciences & Biomedicine ,Mathematics ,0807 Library and Information Studies - Abstract
Made available in DSpace on 2020-12-11T01:57:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-10-29 Griffith University Gowonda HPC Cluster Queensland Cyber Infrastructure Foundation Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research. Griffith Univ, Sch Informat & Commun Technol, Gold Coast, Qld 4222, Australia Griffith Univ, Inst Glyc, Gold Coast, Qld 4222, Australia Univ Colorado, Dept Med Med Oncol, Anschutz Med Campus, Denver, CO USA Tanta Univ, Fac Sci, Bot Dept, Tanta, Egypt Friedrich Schiller Univ, Jena Univ Hosp, Inst Human Genet, Jena, Germany Univ Med Ctr Schleswig Holstein, Dept Radiat Oncol, Campus Kiel, Kiel, Germany ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla, Finland Graz Univ Technol, Inst Environm Biotechnol, Graz, Austria CSIC, CNB, Natl Biotechnol Ctr, Dept Macromol Struct, Madrid, Spain Univ Hong Kong, Fac Dent, Oral & Maxillofacial Radiol Appl Oral Sci & Commu, Hong Kong, Peoples R China Univ Kansas, Div Entomol, Biodivers Inst, Lawrence, KS 66045 USA SUNY Albany, RNA Inst, Albany, NY 12222 USA Robert Koch Inst, Dept Methods Dev & Res Infrastruct, Berlin, Germany Martin Luther Univ Halle Wittenberg, Dept Surg & Conservat Pediat & Adolescent Med, Halle, Germany Indiana Univ Purdue Univ, IU Sch Informat & Comp, Dept BioHlth Informat, Indianapolis, IN 46202 USA Univ Reading, Sch Pharm Stem Cell Biol & Regenerat Med, Reading, Berks, England Dali Univ, Sch Engn, Dali City, Yunnan, Peoples R China Univ Hosp Munich LMU, Dept Psychiat & Psychotherapy, Munich, Germany Univ Tsukuba, Fac Life & Environm Sci, Ibaraki, Japan UniLaSalle, Aghyle, Beauvais, France Henry Ford Hlth Syst, Dept Immunol, Detroit, MI USA Zhejiang Univ, Sch Med, Sir Run Run Shaw Hosp, Dept Emergency, Hangzhou 310016, Zhejiang, Peoples R China Ist Nazl Tumori Fdn Pascale IRCCS, Anesthesia & Pain Med, Naples, Italy NIH, Dept Transfus Med, Bethesda, MD USA Univ Glasgow, Inst Infect Immun & Inflammat, Glasgow, Lanark, Scotland Polish Acad Sci, Inst Human Genet, Poznan, Poland Univ Padua, Dept Surg Oncol & Gastroenterol DISCOG, Padua, Italy Eindhoven Univ Technol, Biomed Engn, Eindhoven, Netherlands Univ Strasbourg, IBMC, Strasbourg, France Heidelberg Univ, Dept Gen Visceral & Transplantat Surg, Heidelberg, Germany Univ Notre Dame, Psychol, Notre Dame, IN 46556 USA Harvard Med Sch, Massachusetts Gen Hosp, Radiol & Pathol, Boston, MA 02115 USA Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA KK Womens & Childrens Hosp, KK Res Ctr, Singapore, Singapore Univ Alabama, Educ Psychol, Tuscaloosa, AL USA UCL, Wellcome EPSRC Ctr Intervent & Surg Sci, London, England Maastricht Univ, Dept Nutr & Movement Sci, Maastricht, Netherlands Univ Arkansas Med Sci, Dept Radiat Oncol, Little Rock, AR 72205 USA Univ Padua, Dept Land Environm Agr & Forestry, Padua, Italy Univ Gothenburg, Dept Biol & Environm Sci, Gothenburg, Sweden Univ Newcastle, Prior Res Ctr Reprod Sci, Callaghan, NSW, Australia Univ Cyprus, Med Sch, Nicosia, Cyprus Shandong Agr Univ, Coll Plant Protect, Agr Big Data Res Ctr, Tai An, Shandong, Peoples R China NIMH, Neuropsychol Lab, Bldg 9, Bethesda, MD 20892 USA Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI USA Univ Oxford, Struct Genom Consortium, Oxford, England Weihenstephan Triesdorf Univ Appl Sci, TUM Campus Straubing Biotechnol & Sustainabil, Bioinformat, Straubing, Germany Univ Michigan, Cardiovasc Res, Ann Arbor, MI 48109 USA Bombay Coll Pharm, Pharmaceut Chem, Mumbai, Maharashtra, India Inst Invest Biol Clemente Estable, Dev Neurobiol, Montevideo, Uruguay Gem Hosp & Res Ctr, Surg Gastroenterol & HPB Surg, Coimbatore, Tamil Nadu, India Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafr Al Sheikh, Egypt Med Univ Innsbruck, Dept Internal Med 4, Innsbruck, Austria Australian Natl Univ, Div Biomed Sci & Biochem, Canberra, ACT, Australia Univ Cattolica Sacro Cuore, Appl Technol Neuropsychol Lab, Milan, Italy Shandong Inst Parasit Dis, Med Entomol, Jinan, Shandong, Peoples R China Kumamoto Univ, Sch Pharm, Kumamoto, Japan Nagoya City Univ, Grad Sch Pharmaceut Sci, Dept Biol Chem, Nagoya, Aichi, Japan Univ Karachi, ICCBS, PCMD, Jamil ur Rahman Ctr Genome Res, Karachi 75270, Pakistan Labormed Zentrum Dr Risch, Vaduz, Liechtenstein Univ Otago, Med, Dunedin, New Zealand KK Womens & Childrens Hosp, Maternal Fetal Med, Singapore, Singapore Albert Ludwigs Univ Freiburg, GERN Tissue Replacement Regenerat & Neogenesis, Dept Orthoped & Trauma Surg, Med Ctr,Fac Med, Freiburg, Germany Heinrich Heine Univ, Inst Biochem & Mol Biol, Dusseldorf, Germany Natl Univ Ireland Galway, Surg, Galway, Ireland Univ Western Australia, Inst Agr, Perth, WA, Australia Univ Oxford, Nuffield Dept Med, Oxford, England SingHlth Polyclin, Punggol Polyclin, Singapore, Singapore Med Univ Innsbruck, Bioctr, Div Biol Chem, Innsbruck, Austria La Trobe Univ, La Trobe Inst Mol Sci, Biochem & Genet, Melbourne, Vic, Australia Univ Bordeaux, INRA, Bordeaux, France Agr & Agri Food Canada, Lethbridge Res & Dev Ctr, Lethbridge, AB, Canada St George Hosp, Trauma & Orthopaed, London, England Max Planck Inst Dev Biol, Dept Prot Evolut, Tubingen, Germany Med Univ Lublin, Dept Gynaecol 2, Lublin, Poland ICIT, ICSI Analyt Natl Res & Dev, Ramnicu Valcea, VL, Romania Univ KwaZulu Natal, Occupat Therapy, Westville Campus, Durban, South Africa Inst Canc Res, Joint Dept Phys, London, England Karolinska Inst, Dept Mol Med & Surg, Solna, Sweden Wenzhou Med Univ, Affiliated Hosp 2, Orthopaed, Wenzhou, Zhejiang, Peoples R China Univ Republica, Fac Ciencias, Ctr Invest Nucl, Lab Virol Mol, Montevideo, Uruguay Univ Gothenburg, Dept Med Biochem & Cell Biol, Gothenburg, Sweden Charite Med Univ Berlin, Pediat Cardiol, Berlin, Germany Bambino Gesu Pediat Hosp, Dept Neurosci & Neurorehabil, Neurorehabil Unit, Rome, Italy Indiana Univ Sch Med Northwest, Microbiol & Immunol, Gary, IN USA Univ Regensburg, Dept Behav & Mol Neurobiol, Regensburg, Germany Univ Queensland, Diamantina Inst, Brisbane, Qld, Australia Translat Res Inst, Brisbane, Qld, Australia Univ Hong Kong, Sch Biomed Sci, Hong Kong, Peoples R China St Jude Childrens Res Hosp, Pharmaceut Sci Dept, 332 N Lauderdale St, Memphis, TN 38105 USA Univ Durham, Mus, Durham, England Univ Ioannina, Med Sch, Dept Hyg & Epidemiol, Ioannina, Greece Tech Univ Munich, Sch Life Sci Weihenstephan, Maximus von Imhof Forum 3, D-85354 Freising Weihenstephan, Germany Univ Hosp Essen, Inst Transfus Med, Essen, Germany Virginia Commonwealth Univ, Comp Sci, Richmond, VA USA Univ Queensland, Inst Social Sci Res, Brisbane, Qld, Australia Univ Lyon, Ctr Int Rech Infectiol, Lyon, France Griffith Univ, Sch Allied Hlth Sci, Gold Coast, Qld, Australia Univ Melbourne, Dept Biochem & Mol Biol, Parkville, Vic, Australia Indiana Univ Purdue Univ, Dept Biohlth Informat, Sch Informat & Comp, Indianapolis, IN 46202 USA Philipps Univ Marburg, Inst Lung Res, Marburg, Germany Indiana Univ, Dept Biol, Bloomington, IN USA Univ Florida, Oral Biol, Gainesville, FL USA Univ Colorado, Dept Biochem, Boulder, CO 80309 USA Kyoto Univ, Ctr Promot Interdisciplinary Educ & Res, Kyoto, Japan Friedrich Loeffler Inst, Inst Virus Diagnost, Greifswald, Germany Univ Oxford, Dept Zool, Oxford, England Tech Univ Munich, Dept Bioinformat, Munich, Germany Univ Adelaide, Sch Anim & Vet Sci, Adelaide, SA, Australia Stanford Univ, Canc Biol, Palo Alto, CA 94304 USA Covenant Univ, Dept Elect & Informat Engn, Ota, Nigeria Heinrich Heine Univ, Inst Stem Cell Res & Regenerat Med, Dusseldorf, Germany Univ British Columbia, Fac Pharmaceut Sci, Vancouver, BC, Canada Albert Einstein Coll Med, Psychiat, New York, NY USA Akdeniz Univ, Med Biol & Genet, Antalya, Turkey NIMH, NIH, Bethesda, MD 20892 USA Med Univ Hosp, Internal Med 1, Tubingen, Germany NET ESolut, Netelabs, Mclean, VA USA Univ Southern Denmark, Inst Publ Hlth, Odense, Denmark John Innes Ctr, Mol Microbiol, Norwich, Norfolk, England Univ Adelaide, Waite Res Precinct, Australian Res Council, Ctr Excellence Plant Energy Biol, Adelaide, SA, Australia Univ Cambridge, Cambridge Inst Publ Hlth, Cambridge, England Univ Munster, Inst Biochem, Munster, Germany Australian Natl Univ, Natl Ctr Epidemiol & Populat Hlth RSPH, Canberra, ACT, Australia Stanford Univ, Neurobiol & Bioengn, Palo Alto, CA 94304 USA Univ Birmingham, Geog Earth & Environm Sci, Birmingham, W Midlands, England Murdoch Univ, Sch Vet & Life Sci, Perth, WA, Australia George Mason Univ, Ctr Climate Change Commun, Fairfax, VA 22030 USA Univ Adelaide, Sch Agr Food & Wine, Adelaide, SA, Australia Southern Med Univ, Sch Pharmaceut Sci, Guangzhou, Guangdong, Peoples R China Stanford Univ, Pathol, Palo Alto, CA 94304 USA Univ Bologna, Dept Expt Diagnost & Specialty Med, Bologna, Italy Univ Padua, Dept Gen Psychol, Padua, Italy Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Dept Bioinformat & Syst Biol, Key Lab Mol Biophys,Minist Educ, Wuhan, Hubei, Peoples R China Huazhong Univ Sci & Technol, Collaborat Innovat Ctr Biomed Engn, Wuhan, Hubei, Peoples R China Queensland Univ Technol, Sch Biomed Sci, Brisbane, Qld, Australia French Natl Ctr Sci Res, CNRS, Inst Biol Physicochim, Paris, France Univ Warwick, Dept Sociol, Coventry, W Midlands, England European Mol Biol Lab, Struct & Computat Biol, Heidelberg, Germany Univ Cincinnati, Coll Med, Canc Biol, Cincinnati, OH USA Univ Waterloo, Biol, Waterloo, ON, Canada Sorbonne Univ, CNRS, Integrat Biol Marine Models LBI2M, SBR, Roscoff, France Univ Manitoba, Biochem & Med Genet, Winnipeg, MB, Canada Max Planck Inst Solid State Res, Ultrafast Solid State Spect, Stuttgart, Germany Imperial Coll London, Sch Publ Hlth, Dept Infect Dis Epidemiol, London, England Mansoura Univ, Fac Vet Med, Biochem, Mansoura, Egypt Univ Virginia, Mol Physiol & Biol Phys, Charlottesville, VA USA China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan, Hubei, Peoples R China Clin Neurol & Psychiat Children & Youth, Child Psychiat, Belgrade, Serbia Ahvaz Jundishapur Univ Med Sci, Med Phys, Ahwaz, Iran Univ Iowa City, Coll Dent, Div Biostat & Computat Biol, Dept Prevent & Community Dent, Iowa City, IA USA Univ Iowa City, Coll Dent, Div Biostat & Computat Biol, Dept Biomed Engn, Iowa City, IA USA Univ Iowa City, Coll Dent, Div Biostat & Computat Biol, Dept Biostat, Iowa City, IA USA Univ Tubingen, Inst Med Psychol & Behav Neurobiol, Tubingen, Germany Univ Ibadan, Dept Zool, Ibadan, Nigeria Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA Univ Tasmania, Sch Technol Environm & Design, Discipline ICT, Hobart, Tas, Australia Childrens Mercy Hosp, Radiol, Kansas City, MO 64108 USA Stockholm Univ, Dept Phys, Stockholm, Sweden Oslo Univ Hosp, Inst Canc Genet & Informat, Oslo, Norway CSIRO, CSIRO Mfg, Pullenvale, Qld, Australia Johns Hopkins Sch Med, Urol, Baltimore, MD USA Univ South Africa, Life & Consumer Sci, Johannesburg, South Africa German Ctr Neurodegenerat Dis, Mech Induced Plast Brain, Bonn, Germany UCL, Inst Child Hlth, Dev Biol & Canc, London, England Simon Fraser Univ, Biomed Physiol & Kinesiol, Burnaby, BC, Canada Univ Manchester, Ctr Hlth Informat, Manchester, Lancs, England Univ Queensland, Sch Human Movement & Nutr Sci, Brisbane, Qld, Australia Albert Einstein Coll Med, Dept Med, New York, NY USA Georgetown Univ, Kennedy Inst Eth, Washington, DC 20057 USA Dermatol Skin & Canc Fdn, Carlton, Vic, Australia Med Coll Wisconsin, Div Hematol, Milwaukee, WI 53226 USA Med Coll Wisconsin, Div Oncol, Milwaukee, WI 53226 USA Med Coll Wisconsin, Div Infect Dis, Milwaukee, WI 53226 USA US FDA, Off Biotechnol Prod, Washington, DC 20204 USA Worcester Polytech Inst, Biomed Engn, Worcester, MA 01609 USA Univ Adelaide, Ctr Orthopaed & Trauma Res, Adelaide, SA, Australia Publ Hlth England, Natl Infect Serv, Bristol, Avon, England Univ Utrecht, Fac Sci Chem, Utrecht, Netherlands Natl Univ Ireland Galway, Pathol, Galway, Ireland Imperial Coll London, NHLI, London, England Danube Private Univ, Neurodegenerat, Krems Donau, Austria Imperial Coll London, Dept Chem, London, England Univ Helsinki, Finnish Museum Nat Hist, Helsinki, Finland Univ Sydney, Sch Life & Environm Sci, Sydney, NSW, Australia Univ Nottingham, Nottingham Digest Dis Ctr, Nottingham, England Univ Toulouse, CNRS, ITAV, USR3505, Toulouse, France ASCR, Inst Mol Genet, CZ Openscreen, Prague, Czech Republic Univ Turku, Inst Biomed, Turku, Finland York Univ, Sch Kinesiol & Hlth Sci, Toronto, ON, Canada Univ Calif Los Angeles, Stein Eye Inst, Ophthalmol, Los Angeles, CA USA Agr & Agri Food Canada, Ottawa Res & Dev Ctr, Ottawa, ON, Canada Helmholtz Zentrum Geesthacht, Mat Design & Characterizat, Geesthacht, Germany Unvers Genova, Internal Med, Genoa, Italy Otto von Guericke Univ, Intelligent Catheter INKS, Magdeburg, Germany Univ Toledo, Canc Biol, 2801 W Bancroft St,Hlth Sci Campus, Toledo, OH 43606 USA CRUK Beatson Inst, Struct Biol, Glasgow, Lanark, Scotland Leibniz Inst Primate Res, Med RNA Biol, Gottingen, Germany Univ Limoges, PEIRENE, EA 7500, Limoges, France Hainan Univ, Vet Med, Haikou, Hainan, Peoples R China UCL, Sch Pharam, Pharmacognosy & Phytotherapy, London, England Univ Oulu, Ecol & Genet Res Unit, Oulu, Finland Rhein Westfal TH Aachen, Inst Biochem & Mol Immunol, Aachen, Germany Univ Stuttgart, Inst Biochem & Tech Biochem, Stuttgart, Germany Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Shenzhen, Peoples R China GIGA Res Inst, Med Oncol, Liege, Belgium CHULiege, Liege, Belgium Fdn Bruno Kessler, MPBA, Trento, Italy Hokkaido Univ, Grad Sch Med, Dept Neurobiol, Sapporo, Hokkaido, Japan Univ Leuven, Oncol, Leuven, Belgium Chalmers Univ Technol, Dept Math Sci, Gothenburg, Sweden Radboud Univ Nijmegen, Med Ctr, Dept Neurol, Nijmegen, Netherlands Univ South Australia, Sch Informat Technol & Math Sci, Adelaide, SA, Australia Bio Thera Solut Ltd, Dept Computat Biol, Guangzhou, Guangdong, Peoples R China Inst Invest Biosanitario Granada IBS, Otolaryngol, Granada, Spain Curtin Univ, Sch Mol & Life Sci, Perth, WA, Australia Newcastle Univ, Inst Cellular Med, Newcastle Upon Tyne, Tyne & Wear, England Goethe Univ, Biol DCAL, Inst Pharm, Frankfurt, Germany Univ Lyon, ICBMS, UMR 5246, Lyon, France Inst Pasteur, Dept Genomes & Genet, Paris, France Univ Valencia, Pharmacol, Valencia, Spain Council Agr Res & Econ, Res Ctr Olive Citrus & Tree Fruit, Caserta, Italy Fraunhofer Inst Toxicol & Expt Med ITEM, Preclin Pharmacol & Vitro Toxicol, Hannover, Germany Univ Tasmania, Sch Med, Hobart, Tas, Australia Chugai Pharmaceut Co Ltd, Oncol Lifecycle Management Dept, Tokyo, Japan Univ Lubeck, Inst Neurobiol, Lubeck, Germany NYU, Sch Med, Neurol, New York, NY USA Univ Putra Malaysia, Dept Plant Pathol, Seri Kembangan, Malaysia Univ N Carolina, Biol, Chapel Hill, NC 27515 USA Univ Southampton, Fac Hlth Sci, Southampton, Hants, England Univ Highlands & Islands, Genet & Immunol Res Grp, Inverness, Scotland Heidelberg Univ, Inst Pathol, Heidelberg, Germany Indraprastha Inst Informat Technol, Dept Computat Biol, New Delhi, India Glasgow Caledonian Univ, Sch Hlth & Life Sci, Glasgow, Lanark, Scotland Liverpool John Moores Univ, Pharm & Biomol Sci, Liverpool, Merseyside, England Parkinsons Inst & Clin Ctr, Basic Res, Sunnyvale, CA USA Luxembourg Inst Sci & Technol, Environm Res & 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Kansas, Med Ctr, Family Med Res Div, Kansas City, KS 66103 USA ASTAR, Inst Mol & Cell Biol, Multimodal Mol Biol, Singapore, Singapore Univ Leuven, Dept Chron Dis Metab & Ageing, Leuven, Belgium Int Iberian Nanotechnol Lab INL, Braga, Portugal Anglia Ruskin, Comp & Technol, Cambridge, England Max Planck Inst Biochem, Struct Cell Biol, Planegg, Germany Univ Seville, Dept Comp Architecture & Technol, Seville, Spain Univ Colorado, EBIO, Boulder, CO 80309 USA Univ Manchester, Canc Sci, Manchester, Lancs, England Australian Natl Univ, Res Sch Populat Hlth, Canberra, ACT, Australia Singapore MIT Alliance Res & Technol, Biosyst, Singapore, Singapore Australian Catholic Univ, Mary MacKillop Inst Hlth Res, Musculoskeletal Hlth & Ageing Res Program, Melbourne, Vic, Australia Ruhr Univ Bochum, Gen Surg, St Josef Hosp, Bochum, Germany Northwest A&F Univ, Coll Life Sci, Xianyang, Shaanxi, Peoples R China Australian Natl Univ, Res Sch Biol, Div Plant Sci, Canberra, ACT, Australia Battelle Mem Inst, Clin & Nonclin Res, Columbus, OH USA Southern Methodist Univ, Biol Sci, Dallas, TX 75275 USA Teikyo Univ, Inst Med Mycol, Tokyo, Japan Tempus Labs, Bioinformat, Chicago, IL USA Hunan Univ, Coll Biol, Changsha, Hunan, Peoples R China Inst Cochin, Dept Infect Immun & Inflammat, Paris, France Royal Coll Surgeons Ireland, FutureNeuro Res Ctr Physiol & Med Phys, Dublin, Ireland Univ Jyvaskyla, Gerontol Res Ctr, Jyvaskyla, Finland Heidelberg Univ, Dept Anesthesiol, Heidelberg, Germany Penn State Univ, Biol, University Pk, PA 16802 USA Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China Michigan State Univ, Anim Sci, E Lansing, MI 48824 USA SUNY Buffalo, Jacobs Sch Med & Biomed Sci, Dept Neurol, Buffalo Neuroimaging Anal Ctr, New York, NY USA Univ Rostock, Inst Biol Sci, Rostock, Germany South China Normal Univ, Sch Environm, Environm Res Inst, Guangzhou, Guangdong, Peoples R China Univ Bari, Dept Vet Med, Bari, Italy Radboud Univ Nijmegen, Med Ctr, Primary & Community Care, Nijmegen, Netherlands EcoHlth Alliance, New York, NY USA Mayo Clin, Cardiovasc Dept, Rochester, MN USA Med Univ Silesia, Sch Med Katowice, Dept Epidemiol, Katowice, Poland Univ Lleida, Anim Sci, Lleida, Spain Univ Florida, Coll Pharm, Pharmaceut Outcomes & Policy, Gainesville, FL USA Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99352 USA Univ Edinburgh, Ctr Discovery Brain Sci, Edinburgh, Midlothian, Scotland Harvard Med Sch, Dept Biomed Informat, Boston, MA 02115 USA Univ Penn, Radiol, Philadelphia, PA 19104 USA Humanitas Univ & Res Hosp, Asthma & Allergy Unit, Biomed Sci Personalized Med, Rozzano, Italy Australian Natl Univ, Dept Quantum Sci, Canberra, ACT, Australia Carl von Ossietzky Univ Oldenburg, Ecol Genom, Oldenburg, Germany Int Med Univ, Paediat Dent & Orthodont, Kuala Lumpur, Malaysia Univ Ottawa, Sch Nursing, Ottawa, ON, Canada Maastricht Univ, Dept Educ Support, Maastricht, Netherlands Univ Manchester, Math, Manchester, Lancs, England Kings Coll London, Fac Life Sci & Med, Sch Populat Hlth Sci, London, England Queensland Univ Technol, Inst Hlth & Biomed Innovat, Brisbane, Qld, Australia Harbin Med Univ, Publ Hlth Sch, Epidemiol, Harbin, Heilongjiang, Peoples R China UiT Arctic Univ Norway, Dept Chem, Tromso, Norway Cornell Univ, Biol Stat & Computat Biol, Ithaca, NY USA East China Normal Univ, Sch Ecol & Environm Sci, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Shanghai, Peoples R China Johannes Gutenberg Univ Mainz, Fac Biol, Mainz, Germany Univ Massachusetts, Food Sci, Amherst, MA 01003 USA Max Planck Inst Terr Microbiol, Complex Adapt Traits Res Grp, Marburg, Germany NIHR Biomed Res Ctr Resp, Ctr Exercise & Rehabil Sci, Leicester, Leics, England Yale Univ, Dept Internal Med, Sect Endocrinol, New Haven, CT USA Sardar Patel Univ, Dept Biosci, Anand, Gujarat, India Univ Tokyo, Dept Appl Phys, Tokyo, Japan Univ Cologne, Vivo Res Facil ivRF, Cologne Excellence Cluster Cellular Stress Respon, Cologne, Germany Natl Open Univ Nigeria, Dept Publ Hlth Sci, Lagos, Nigeria Univ Hosp Regensburg, Dept Radiol, Regensburg, Germany Natl Univ Singapore, Geog, Singapore, Singapore Alfaisal Univ, Coll Med, Riyadh, Saudi Arabia Abdelmalek Essaadi Univ, Fac Sci & Techn Tangier, Biomed Genom & Oncogenet Res Lab, Tetouan, Morocco Tech Univ Munich, Fac Sport & Hlth Sci, Exercise Biol Grp, Munich, Germany Univ Santiago de Compostela, Dept Psicoloxia Social Basica & Metodoloxia, Galiza, Spain Griffith Univ, Signal Proc Lab, Brisbane, Qld, Australia Univ Ghent, Dept Neurol, Ghent, Belgium Ghent Univ Hosp, Ghent, Belgium Univ Ghent, Fac Vet Med, Merelbeke, Belgium Albert Einstein Coll Med, Inst Clin & Translat Res, New York, NY USA Anglia Ruskin Univ, Fac Med Sci, Cambridge, England Peking Univ, Coll Chem & Mol Engn, Beijing, Peoples R China Herlev & Gentofte Hosp, Dept Dermatol & Allergy, Hellerup, Denmark Lady Cilento Childrens Hosp, Med Imaging & Nucl Med, Brisbane, Qld, Australia Univ Gambia, Sch Med & Allied Hlth Sci, Nursing & Reprod Hlth, Brikama, Gambia Univ Sydney, Woolcock Inst Med Res, Sydney, NSW, Australia Wayne State Univ, Comp Sci, Detroit, MI USA Aberdeen Royal Infirm, Otolaryngol, Aberdeen, Scotland Univ Toronto, Lab Med & Pathobiol, Toronto, ON, Canada Chinese Univ Hong Kong, Inst Ageing, Hong Kong, Peoples R China Univ Nebraska Med Ctr, Emergency Med, Omaha, NE USA Univ Florida, Coll Med, Pathol, Gainesville, FL USA Univ Texas MD Anderson Canc Ctr, Radiat Oncol, Houston, TX 77030 USA Univ Pisa, Translat Res NTMS, Pisa, Italy Magna Grecia Univ, Clin & Expt Med, Catanzaro, Italy Univ Vermont, Larner Coll Med, Pediat, Burlington, VT USA Sun Yat Sen Univ, Canc Ctr, Diagnost & Intervent Ultrasound, Guangzhou, Guangdong, Peoples R China Fudan Univ, Inst Brain Sci, Shanghai, Peoples R China Shanxi Agr Univ, Coll Agron, Jinzhong, Shanxi, Peoples R China Univ Toronto, Inst Med Sci, Toronto, ON, Canada Univ Adelaide, ARCPOH, Adelaide, SA, Australia Pelita Harapan Univ, Fac Med, Cardiol & Vasc Med, Tangerang, Indonesia Inst Mental Hlth, Res Div, Singapore, Singapore UCL, MRC Clin Trials Unit, London, England Univ Padua, Dept Philosophy Sociol Educ & Appl Psychol FISPPA, Padua, Italy Univ Lubeck, Dept Psychiat & Psychotherapy, Lubeck, Germany Dalian Univ Technol, Ctr Mol Med, Dalian, Liaoning, Peoples R China Tianjin United Family Healthcare, Reprod Med, Tianjin, Peoples R China Univ Autonoma Barcelona, Dept Engn Quim Biol & Ambiental, Barcelona, Spain Sao Paulo State Univ UNESP, Dept Anim Sci, Sao Paulo, Brazil Helmholtz Zentrum Munchen, Inst Computat Biol, Canc Syst Biol, Ingolstadter Land Str 1, D-85764 Munich, Germany Univ Tokyo, Dept Cardiovasc Med, Tokyo, Japan Sao Paulo State Univ, Vet Clin, Sao Paulo, Brazil Zhejiang Univ, Inst Biotechnol, Hangzhou, Zhejiang, Peoples R China Aarhus Univ, Dept Mol Biol & Genet, Aarhus, Denmark Univ Manchester, St Marys Hosp, Maternal & Fetal Hlth, Manchester, Lancs, England Univ New Mexico, Internal Med, Albuquerque, NM 87131 USA Mayo Clin, Div Biomed Stat & Informat, Jacksonville, FL 32224 USA King Saud Univ, Plant Prod, Riyadh, Saudi Arabia Polish Acad Sci, Inst Genet & Anim Breeding, Dept Mol Biol, Warsaw, Poland Queensland Univ Technol, Optometry & Vis Sci, Brisbane, Qld, Australia Univ Nottingham, Sch Life Sci, Nottingham, England Canc Council Queensland, Canc Res Ctr, Brisbane, Qld, Australia Umea Univ, Dept Chem, Umea, Sweden Publ Hlth England, Ctr Radiat Chem & Environm Hazards, Bristol, Avon, England Univ Campania L Vanvitelli, DISTABIF, Caserta, Italy Bartshealth, Obstet & Gyanecol, London, England Univ Clermont Auvergne, GReD Lab, Clermont Ferrand, France INRA Abeilles & Environm, Avignon, France Queensland Univ Technol, Sch Publ Hlth & Social Work, Brisbane, Qld, Australia Univ Penn, Psychiat, Philadelphia, PA 19104 USA Novosibirsk State Univ, Res Inst Physiol & Basic Med, Novosibirsk, Russia UCL, Dept Chem, London, England La Trobe Univ, Anim Plant & Soil Sci, Melbourne, Vic, Australia Univ Arizona, Epidemiol & Biostat, Tucson, AZ USA Univ Groningen, Univ Med Ctr Groningen, ERIBA, Groningen, Netherlands Univ Gothenburg, Inst Clin Sci, Dept Radiat Phys, Gothenburg, Sweden SUNY Upstate Med Univ, Biochem & Mol Biol, Syracuse, NY 13210 USA Fundacao Oswaldo Cruz, Ctr Pesquisas Goncalo Moniz, Salvador, Bahia, Brazil Inst Environm Sci & Res ESR, Food Water & Environm Microbiol, Christchurch, New Zealand Univ Otago, Food Sci, Dunedin, New Zealand Florida Atlantic Univ, Biomed Sci, Boca Raton, FL 33431 USA Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia Univ Hosp Wurzburg, Inst Clin Neurobiol, Wurzburg, Germany Inst Trop Med, Publ Hlth, Antwerp, Belgium Univ Thessaly, Sch Hlth Sci, Fac Med, Dept Rheumatol & Clin Immunol, Larisa, Greece Univ Basel, UZB Univ Ctr Dent Med, Dept Orthodont & Pediat Dent, Basel, Switzerland Sorbonne Univ, Museum Natl Hist Nat, Inst Systemat Evolut Biodiversite ISYEB, MNHN,CNRS,UMR 7205,EPHE, Paris, France CNRS, Inst Adv Biosci, Paris, France Univ Western Australia, Sch Mol Sci, Perth, WA, Australia Univ Ft Hare, Biochem & Microbiol, Alice, South Africa Univ Tubingen, Phys, Tubingen, Germany Griffith Univ, Sch Environm & Sci, Brisbane, Qld, Australia Philosoph Theol Hsch Vallendar, Stat & Standardised Methods, Vallendar, Germany Imperial Coll London, Life Sci, London, England Univ Adelaide, Adelaide Med Sch, Adelaide, SA, Australia Univ Nebraska Med Ctr, Dept Pharmacol & Expt Neurosci, Omaha, NE USA Technol Univ Dublin, FOCAS Res Inst, Dublin, Ireland INSERM, Natl Inst Hlth & Med Res, Canc Res Ctr Toulouse, Paris, France Univ Santiago de Compostela, Dept Bioloxia Func, Grp BRAINSHARK, Galiza, Spain Univ Nebraska, Biol, Kearney, NE USA Univ Autonoma Barcelona, Dept Genet & Microbiol, Barcelona, Spain Univ Pisa, Chem & Ind Chem, Pisa, Italy Univ Manchester, Wellcome Trust Ctr Cell Matrix Res, Manchester, Lancs, England Univ Montpellier, CNRS, Inst Human Genet, Montpellier, France Czech Acad Sci, Inst Organ Chem & Biochem, Prague, Czech Republic CSIC, Natl Ctr Biotechnol CNB, Computat Syst Biol Grp, Madrid, Spain Natl Cheng Kung Univ, Dept Biochem & Mol Biol, Tainan, Taiwan Harvard Med Sch, Schepens Eye Res Inst, Ophthalmol, Boston, MA 02115 USA Lanzhou Univ, Hosp 2, Dept Gen Surg, Lanzhou, Gansu, Peoples R China Univ Technol Sydney, Sch Life Sci, Sydney, NSW, Australia JT Chen Clin, Gynecol, Tokyo, Japan Univ Western Australia, Sch Agr & Environm, Perth, WA, Australia Beijing Normal Univ, Fac Geog Sci, Beijing, Peoples R China Univ Missouri, Elect Engn & Comp Sci, Columbia, MO USA Vrije Univ Amsterdam Med Ctr, Amsterdam Publ Hlth Res Inst, Dept Publ & Occupat Hlth, Amsterdam, Netherlands Semmelweis Univ, Med Biochem, Budapest, Hungary Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China Univ Pittsburgh, Chem, Pittsburgh, PA USA GB Pant Inst Post Grad Med Educ & Res, Neurol, New Delhi, India Univ Copenhagen, Vet & Anim Sci, Copenhagen, Denmark Univ Palermo, Biomed Dept Internal & Specialist Med DIBIMIS, Sect Endocrinol, Palermo, Italy UCL, NPP, London, England Univ Florida, Microbiol & Cell Sci, Gainesville, FL USA Univ Salford, Sch Hlth Sci, Manchester, Lancs, England Cardiff Univ, Inst Med Genet, Cardiff, S Glam, Wales INSERM, ICO Canc Ctr, Angers, France Univ Colombo, Fac Med, Microbiol, Colombo, Sri Lanka Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing, Peoples R China Univ Porto, Fac Pharm, Porto, Portugal Univ Nottingham, Div Primary Care, Nottingham, England Univ Illinois, Pharm Syst Outcomes & Policy, Chicago, IL USA Pontificia Univ Catolica Goias, Escola Ciencias Agr & Biol, Goiania, Go, Brazil China Japan Friendship Hosp, Dept Oncol, Beijing, Peoples R China Boston Univ, Chem, Boston, MA 02215 USA Amer Univ, Environm Sci, Washington, DC 20016 USA Carleton Univ, Neurosci, Ottawa, ON, Canada Univ Regina, Chem & Biochem, Regina, SK, Canada Univ Montreal, Microbiolgy, Montreal, PQ, Canada Univ Leicester, Dept Genet & Genome Biol, Leicester, Leics, England Univ Queensland, Sch Agr & Food Sci, Gatton, Qld, Australia CNRS, BIOM, Paris, France UCL, Hatter Cardiovasc Inst, London, England Flinders Univ S Australia, Sci & Engn, Adelaide, SA, Australia Univ Warwick, Engn, Coventry, W Midlands, England Katholieke Univ Leuven, Ctr Human Genet, Leuven, Belgium Fudan Univ, Dept Macromol Sci, Shanghai, Peoples R China Dokuz Eylul Univ, Biol Educ, Izmir, Turkey Indiana Univ Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA Mondo Med, Pulm Dis, Borgomanero, Italy US Naval, Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC USA Univ Oslo, Inst Basic Med Sci, Dept Nutr, Oslo, Norway Peking Univ, Dept Mech & Engn Sci, Beijing, Peoples R China Saarland Univ, Dept Pharm Pharmaceut & Med Chem, Saarbrucken, Germany INSERM, Natl Inst Hlth & Med Res, Skin Res Inst, Paris, France Shanghai Proton & Heavy Ion Ctr, Res & Dev, Shanghai, Peoples R China Univ Georgia, Epidemiol, Athens, GA 30602 USA Univ Freiburg, Med Ctr, Dept Plast & Hand Surg, Freiburg, Germany Sidra Med, Res, Doha, Qatar Rostock Univ, Med Ctr, Dept Oral Maxillofacial & Plast Surg, Rostock, Germany Harvard Med Sch, Brigham & Womens Hosp, Emergency Med, Boston, MA 02115 USA AgResearch, Forage Sci, Palmerston North, New Zealand Univ Calif Los Angeles, Med, Los Angeles, CA USA Arizona State Univ, Biodesign Inst, Virginia G Piper Ctr Personalized Diagnost, Tempe, AZ USA Sheffield Hallam Univ, Res Inst, Mat & Engn, Sheffield, S Yorkshire, England Aneurin Bevan Univ Healthboard, Resp Med, Newport, Shrops, England Univ Calif San Francisco, Neurol Surg, San Francisco, CA 94143 USA Univ Western Australia, UWA Dent Sch, Perth, WA, Australia Fordham Univ, Biol Sci, Bronx, NY 10458 USA Univ Helsinki, Inst Biotechnol, Helsinki, Finland Fujian Normal Univ, Coll Life Sci, Fuzhou, Fujian, Peoples R China Univ Fukui, Dept Frontier Fiber Technol & Sci, Fukui, Japan Univ Southampton, Fac Med, Clin & Expt Sci, Southampton, Hants, England Univ Urbino, Dept Biomol Sci, Urbino, Italy Osped San Luigi, Allergol Unit, Turin, Italy Muljibhai Patel Urol Hosp, Dept Urol, Nadiad, Gujarat, India Univ Granada, Stratig & Paleontol, Granada, Spain Massey Univ, Sch Vet Sci, Auckland, New Zealand CNR, High Performance Comp & Networking Inst, Naples, Italy Univ Childrens Hosp Zurich, Div Metab, Zurich, Switzerland Univ Childrens Hosp Zurich, Childrens Res Ctr, Zurich, Switzerland Univ Melbourne, Biochem & Mol Biol, Parkville, Vic, Australia Inst Pasteur, Leptospirosis Res & Expertise Unit, Noumea, New Caledonia Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China Cheikh Anta Diop Univ UCAD, Sci Fac, Biol Anim Dept, Dakar, Senegal Providence Portland Med Ctr, Earle A Chiles Res Inst, Portland, OR USA Agr & Agri Food Canada, Lacombe Res & Dev Ctr, Lacombe, AB, Canada Alberta Innovates, Performance Management & Evaluat, Edmonton, AB, Canada Univ Malaga, CSIC, Inst Hortofruticultura Subtrop Mediterranea La Ma, IHSM,UMA, Malaga, Spain James Cook Univ, Coll Publ Hlth Med & Vet Sci, Cairns, Qld, Australia European Commiss, Joint Res Ctr, Ispra, Italy Univ Montpellier, Montpellier, France CSIRO, Floreat, WA, Australia Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou, Guangdong, Peoples R China Vanderbilt Univ, Pharmacol, 221 Kirkland Hall, Nashville, TN 37235 USA RIKEN, KFU RIKEN Translat Genom Unit, Yokohama, Kanagawa, Japan Univ Tubingen, Neurobiol Vocal Commun, Tubingen, Germany Univ Colorado, UCCS Ctr Biofrontiers Inst, Colorado Springs, CO 80907 USA Fred Hutchinson Canc Ctr, Div Basic Sci, Seattle, WA USA Univ Geneva, Fac Med, Primary Care Unit, Geneva, Switzerland Ernst Moritz Arndt Univ Greifswald, Dept Mol Genet & Infect Biol, Greifswald, Germany East China Univ Sci & Technol, Dept Fine Chem, Shanghai, Peoples R China Ohio State Univ, Surg, Columbus, OH 43210 USA Oragenics, R&D, Tampa, FL USA Natl Environm Agcy, Environm Hlth Inst, Singapore, Singapore Friedrich Loeffler Inst, Inst Diagnost Virol, Greifswald, Germany Queen Elizabeth Hosp, Oncol, Woodville, SA, Australia USDA, Emerging Pests & Pathogens Res Unit, Ithaca, NY USA Univ Freiburg, Med Ctr, Dept Neurosurg, Epilepsy Ctr, Freiburg, Germany Univ Hong Kong, Fac Educ, Informat & Technol Studies, Hong Kong, Peoples R China Univ Tokyo, Grad Sch Agr & life Sci, Agr & Environm Biol, Tokyo, Japan Univ Pittsburgh, Dept Med, Pittsburgh Heart Lung & Blood Vasc Med Inst, Pittsburgh, PA USA Guys & St Thomas NHS Fdn Trust, Directorate Transplant Renal & Urol, London, England Univ Sarajevo, Clin Ctr, Clin Heart Blood Vessel & Rheumat Dis, Sarajevo, Bosnia & Herceg Univ Kent, Sch Math Stat & Actuarial Sci, Canterbury, Kent, England Tokyo Inst Technol, Dept Life Sci & Technol, Tokyo, Japan Univ Appl Sci Munich, Laser Ctr Dept Appl Sci & Mechatron, Munich, Germany CIC NanoGUNE, Nanodevices, San Sebastian, Spain Vrije Univ Amsterdam Med Ctr, Gynaecol, Amsterdam, Netherlands Cardiff Univ, Med Sch, Div Populat Med, Cardiff, S Glam, Wales Karolinska Inst, Dept Med, Solna, Sweden Natl Inst Genet, Ctr Informat Biol, Mishima, Shizuoka, Japan Murdoch Univ, Harry Perkins Inst Med Res, Perth, WA, Australia Univ Limerick, Phys Educ & Sport Sci, Limerick, Ireland Ruhr Univ Bochum, Campus Clin Gynecol, Univ Str, Bochum, Germany Southwest Med Univ, Sch Publ Hlth, Epidemiol & Biostat, Luzhou, Sichuan, Peoples R China Beijing Canc Hosp, Minist Educ Beijing, Key Lab Carcinogenesis & Translat Res, Ctr Mol Diagnost, Beijing, Peoples R China Chinese Acad Sci, Chengdu Inst Biol, Herpetol Dept, Chengdu, Sichuan, Peoples R China Key Lab Nano Biol Effects & Safety, Beijing, Peoples R China NIBR, PK Sci, Basel, Switzerland Daejeon St Marys Hosp, Pain Ctr, Daejeon, South Korea Univ Western Ontario, Sch Hlth Studies, London, ON, Canada Univ Aberdeen, Hlth Psychol Grp, Aberdeen, Scotland Univ Colorado, Anesthesiol, Anschutz Med Campus, Boulder, CO 80309 USA Univ Antwerp, UZA Antwerp Univ Hosp, Crit Care Med, Edegem, Belgium Aarhus Univ Hosp, Endocrinol, Aarhus, Denmark Univ Pretoria, Ctr Transport Dev, Ind & Syst Engn, Pretoria, South Africa Duke Univ, Dept Biostat & Bioinformat, Durham, NC USA Pontificia Univ Catolica Goias, Med Pharmaceut & Biomed Sci Sch, Goiania, Go, Brazil Karolinska Inst, Danderyd Hosp, Dept Clin Sci, Div Cardiovasc Med, Stockholm, Sweden UCL, Clin Educ & Hlth Psychol, London, England KRIBB, Dev & Differentiat Res Ctr, Daejeon, South Korea Univ dArtois, Lab Barriere Hematoencephal, Arras, France AstraZeneca, IMED Biotech Unit, Discovery Sci, Quantitat Biol, Cambridge, England Pacific Northwest Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA Coventry Univ, Appl Maths Res Ctr, Stat Phys Grp, Coventry, W Midlands, England Wilton Ctr, Invista Performance Technol, Cleveland, England Thunen Inst Forest Genet, Genome Res, Grosshansdorf, Germany Lebanese Amer Univ, Nat Sci, Byblos, Lebanon Takeda, Evidence & Value Generat, Osaka, Japan King Abdullah Int Med Res Ctr, Stem Cell & Regenerat Med, Riyadh, Saudi Arabia Univ Bahri, Ind Pulp & Paper, Khartoum, Sudan Univ Queensland, Mater Med Res Inst, Mater Res Inst, Brisbane, Qld, Australia Colorado State Univ, NREL, Ft Collins, CO 80523 USA Rzeszow Univ Hosp, Ob Gyn Dept, Rzeszow, Poland Univ Leeds, Fac Biol Sci, Leeds, W Yorkshire, England Massachusetts Gen Hosp, Endocrine Unit, Boston, MA 02114 USA Carl von Ossietzky Univ Oldenburg, Neurosci, Oldenburg, Germany UNSW, St George & Sutherland Clin Sch, Microbiome Res Ctr, Sydney, NSW, Australia NYU, Sch Med, Dept Biochem, New York, NY 10016 USA NYU, Sch Med, Dept Mol Pharmacol, New York, NY USA Univ Mississippi, Med Ctr, Med Infect Dis, Jackson, MS 39216 USA Tampere Univ, Fac Social Sci Psychol, Tampere, Finland Univ Cyprus, Dept Biol Sci, Nicosia, Cyprus Goethe Univ, Inst Med Microbiol & Infect Control, Frankfurt, Germany Charite Med Univ Berlin, Inst Radiol, Berlin, Germany Univ Cologne, Univ Hosp Cologne, Internal Med 1, Cologne, Germany Univ Calif Los Angeles, Sch Dent, Sect Periodont, Los Angeles, CA 90024 USA Aalborg Univ Hosp, Dept Clin Biochem, Aalborg, Denmark Manipal Acad Higher Educ, Pharm Practice, Manipal, Karnataka, India Univ Tokyo, Inst Med Sci, Dept Radiol, Tokyo, Japan Cukurova Univ, Family Med, Fac Med, Adana, Turkey Catholic Univ Korea, Coll Med, Dept Humanities & Social Med, Seoul, South Korea Univ Ghent, Food Technol Safety & Hlth, Ghent, Belgium UNSW Sydney, Sch Biol Earth & Environm Sci BEES, Sydney, NSW, Australia St Vincent Shoulder & Sports Clin, Res Unit, Vienna, Austria Cornell Univ, Biomed Engn, Ithaca, NY USA Leibniz Inst Plant Genet & Crop Plant Res IPK, Res Grp Bioinformat & Informat Technol, Gatersleben, Germany Univ Europea Madrid, Sch Doctoral Studies, Madrid, Spain CSIRO Mfg, Biomed Mfg, Melbourne, Vic, Australia Depaul Univ, Biol Sci, Chicago, IL 60604 USA Konkuk Univ, Dept Anim Sci & Technol, Seoul, South Korea Chang Gung Univ, Grad Inst Med Mechatron, Taoyuan, Taiwan Korea Univ, Coll Med, Psychiat, Seoul, South Korea Princeton Univ, Chem, Princeton, NJ 08544 USA Henan Univ Chinese Med, Henan Key Lab Chinese Med Resp Dis, Zhengzhou, Henan, Peoples R China Univ Med Ctr Mainz, Dept Psychiat & Psychotherapy, Mainz, Germany Monash Univ Malaysia, Sch Sci, Selangor, Malaysia Univ Mississippi, Med Ctr, Physiol & Biophys, Jackson, MS 39216 USA Univ Oslo, Dept Transplantat Med, Oslo, Norway Sichuan Agr Univ, Triticeae Res Inst, Yaan, Sichuan, Peoples R China Guangzhou Univ Chinese Med, Gastroenterol, Guangzhou, Guangdong, Peoples R China Southeast Univ, Sch Biol Sci & Med Engn, Suzhou, Jiangsu, Peoples R China Mt Allison Univ, Biol, Sackville, NB, Canada Ithaca Coll, Biol, Ithaca, NY 14850 USA Univ Cagliari, Dept Med Sci & Publ Hlth, Monserrato, Italy Univ Vienna, Chromosome Biol, Vienna, Austria Univ Zurich, Nephrol, Zurich, Switzerland Friedrich Schiller Univ, Inst Nutr Sci, Jena, Germany UNSW Sydney, Grad Sch Biomed Engn, Sydney, NSW, Australia Tulane Univ, Sch Med, Biochem & Mol Biol, 1430 Tulane Ave, New Orleans, LA 70112 USA NINDS, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA NIH, NCBI, Natl Lib Med, Bldg 10, Bethesda, MD 20892 USA LOreal Res & Innovat, Aulnay Sous Bois, France Lund Univ, Dept Psychol, Malmo, Sweden Catholic Univ Louvain, Inst Rech Expt & Clin, Brussels, Belgium Georgia State Univ, Neurosci Inst, Atlanta, GA 30303 USA Univ Melbourne, Ophthalmol, Surg, Parkville, Vic, Australia Univ Western Australia, Ctr Ophthalmol & Visual Sci, Perth, WA, Australia Iran Univ Med Sci, Med Phys, Fac Med, Tehran, Iran Salk Inst Biol Studies, Cellular Neurobiol, La Jolla, CA USA Imperial Coll London, Fibrosis Res Grp, London, England Univ Texas MD Anderson Canc Ctr, Genitourinary Med Oncol, Houston, TX 77030 USA Univ Liege, GIGA Neurosci, Liege, Belgium Univ Crete, Sch Med, Urol, Iraklion, Greece Flinders Univ S Australia, Flinders Med Ctr, Dept Clin Pharmacol, Adelaide, SA, Australia Max Planck Inst Immunobiol & Epigenet, Bioinformat, Breisgau, Germany Cardiff Univ, Sch Psychol, Cardiff, S Glam, Wales Imperial Coll London, Chem Engn, London, England Lund Univ, Skane Univ Hosp, Clin Sci, Malmo, Sweden Sahlgrens Acad, Inst Clin Sci, Dept Mol & Clin Med, Gothenburg, Sweden Univ Cent Lancashire, Sch Pharm & Biomed Sci, Preston, Lancs, England Hosp Univ Doctor Peset, Psychiat & Clin Psychol, Valencia, Spain Ctr Biol Mol Severo Ochoa, Genome Dynam & Funct, Madrid, Spain Unvivers Hosp Lille, Dept Intens Care, Lille, France Kansai Med Univ, Surg, Osaka, Japan Univ Toulouse, Inst Natl Polytech Toulouse, Ecole Natl Super Agron Toulouse, Lab Genom & Biotechnol Fruit, Toulouse, France UiT Arctic Univ Norway, Inst Psychol, Tromsto, Norway Queens Univ, Ctr Publ Hlth, Belfast, Antrim, North Ireland Univ Manchester, Ctr Primary Care & Hlth Serv Res, Manchester, Lancs, England Griffith Univ, Menzies Hlth Inst, Gold Coast, Qld, Australia Anglia Ruskin Univ, FHSCE, Cambridge, England Univ Lleida, Dept Expt Med, Lleida, Spain NIEHS, Biomol Screening Branch, Div Natl Toxicol Program, POB 12233, Res Triangle Pk, NC 27709 USA Albany Med Coll, Immunol & Microbial Dis, Albany, NY 12208 USA Univ Queensland, Queensland Brain Inst, Brisbane, Qld, Australia Univ Kent, Sch Biosci, Canterbury, Kent, England Univ Bourgogne Franche Comte, INSERM, LNC, UMR 1231, Besancon, France Ritsumeikan Univ, Coll Life Sci, Dept Biotechnol, Shiga, Japan Kent State Univ, Biol Sci, Kent, OH 44242 USA Natl Inst Infect Dis, Dept Safety Res Blood & Biol Prod, Tokyo, Japan European Inst Marine Studies, Lab Microbiol Extreme Environm, Plouzane, France Univ Iowa, Dept Pharmacol, Roy J & Lucille A Carver Coll Med, Iowa City, IA 52242 USA Natl Univ Singapore, Biol Sci, Singapore, Singapore Conservatoire Natl Arts & Metiers, Lab GBA, EA4627, Paris, France Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow, Russia Jikei Univ, Sch Med, Dept Mol Biol, Tokyo, Japan Univ South Wales, 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Phys & Mech Engn, Brisbane, Qld, Australia Otto von Guericke Univ, Psychol, Magdeburg, Germany Univ Med Ctr Gottingen, Dept Expt Neurodegenerat, Gottingen, Germany Harvard Med Sch, Spaulding Rehabil Hosp, Phys Med & Rehabil, Boston, MA 02115 USA Quadram Inst Biosci, Sci Operat, Norwich, Norfolk, England Ostbayer Tech Hsch Regensburg OTH Regensburg, Regensburg Med Image Comp ReMIC, Regensburg, Germany Deakin Univ, Fac Arts & Educ, Melbourne, Vic, Australia Univ Warwick, Warwick Med Sch, Coventry, W Midlands, England INSERM, Natl Inst Hlth & Med Res, Biochem & Mol Biol, Paris, France Univ Liege, Tax Inst, Liege, Belgium Univ Leeds, Sch Mol & Cellular Biol, Leeds, W Yorkshire, England IRCCS Ist Giannina Gaslini, UOC Genet Med, Genoa, Italy Res Diets Inc, Sci, New Brunswick, NJ USA Univ Perugia, Dept Phys & Geol, Perugia, Italy Walter Reed Natl Mil Med Ctr, Cellular Immunol, Bethesda, MD USA Univ Fed Santa Catarina, Biol Sci Ctr, Microbiol Immunol & Parasitol Dept, Florianopolis, SC, Brazil Univ Edinburgh, Royal Infirm, Ctr Liver & Digest Disorders, Edinburgh, Midlothian, Scotland Orion Pharma, Crit Care Proprietary Prod Div, Espoo, Finland MIT, Dept Civil & Environm Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA Univ Turin, Dept Vet Sci, Turin, Italy Univ G dAnnunzio, Dept Psychol Hlth & Territorial Sci, Chieti, Italy NYU, Sch Med, OB GYN, New York, NY USA Univ Glasgow, Inst Cardiovasc & Med Sci, Glasgow, Lanark, Scotland Univ Turku, Dept Biol, Turku, Finland Tech Univ Berlin, Bioanalyt, Berlin, Germany Univ Goettingen, Inst Phys Biophys 3, Gottingen, Germany Univ Texas MD Anderson Canc Ctr, Inst Appl Canc Sci, Translat Res Adv Therapeut & Innovat Oncol TRACTI, Houston, TX 77030 USA Univ Liege, Life Sci, Liege, Belgium Tarbiat Modares Univ, Fac Med Sci, Dept Toxicol, Tehran, Iran ARS, USDA, Stoneville, MS USA Univ Regensburg, RCI Regensburg Ctr Intervent Immunol, Regensburg, Germany Univ Nottingham, Sch Psychol, Nottingham, England NIH, Pathol Lab, Bethesda, MD 20892 USA Univ Carlos III Madrid, Elect Engn, Madrid, Spain Inst Med Mol, Chem Biol, Lisbon, Portugal Univ Costa Rica, CIET, San Jose, Costa Rica Univ Stavanger, Fac Hlth Sci, Stavanger, Norway Erasmus MC, Urol, Rotterdam, Netherlands Univ Edinburgh, Sch Biol Sci, Edinburgh, Midlothian, Scotland German Res Ctr Environm Hlth GmbH, Helmholtz Zentrum Munchen, Inst Bioinformat & Syst Biol IBIS, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany Leiden Univ, Huygens Kamerlingh Onnes Lab, Leiden, Netherlands Univ Vienna, Nutr Sci, Vienna, Austria Kolling Inst Med Res, Med, St Leonards, NSW, Australia Johns Hopkins Sch Med, Biol Chem, Baltimore, MD USA Univ Montreal, Med Nutr & Microbiome Lab, Montreal, PQ, Canada GlaxoSmithKline, Cell & Gene Therapy, Stevenage, Herts, England Univ Trieste, Life Sci, Trieste, Italy Rhein Westfal TH Aachen, Dept Radiol, Aachen, Germany Univ Duisburg Essen, Univ Hosp Essen, West German Canc Ctr, Dept Med Oncol, Essen, Germany Med Univ Vienna, Obstet & Gynecol, Vienna, Austria FHI 360, Social & Behav Hlth Sci Div, Washington, DC USA KU Leuven VIB, Switch Lab, Leuven, Belgium Bielefeld Univ, Fac Technol, Bielefeld, Germany Capital Med Univ, Beijing Shijitan Hosp, Dept Clin Nutr, Dept Gastrointestinal Surg, Beijing, Peoples R China Meiji Univ, Dept Agr Chem, Kawasaki, Japan Yonsei Univ, Coll Med, Microbiol, Seoul, South Korea Johnson & Johnson EAME, Maidenhead, Berks, England Penn State Coll Med, Pediat, Hershey, PA USA Univ N Carolina, Dept Social Med, Chapel Hill, NC 27515 USA Univ Western Australia, ARC CoE Plant Energy Biol, Perth, WA, Australia Wageningen Univ, Div Human Nutr & Hlth, Wageningen, Netherlands Kings Coll London, Dept Neuroimaging, London, England Univ Murcia, Biochem & Mol Biol, Murcia, Spain Old Dominion Univ, Dept Biol Sci, Norfolk, VA 23529 USA Monash Univ, Biochem & Mol Biol, Melbourne, Vic, Australia Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China Univ Pittsburgh, Pharmacol & Chem Biol, Pittsburgh, PA USA Univ Lausanne, Ctr Integrat Genom, Lausanne, Switzerland Univ Queensland, Sch Pharm, Brisbane, Qld, Australia Leibniz Inst Plant Genet & Crop Plant Res IPK Gat, Genebank, Gatersleben, Germany Piramal Imaging, Res & Dev, Berlin, Germany Univ Leeds, Civil Engn, Leeds, W Yorkshire, England Univ Missouri, Chem & Biochem, St Louis, MO 63121 USA US Geol Survey, Coastal & Marine Geol Program, Pacific Coastal & Marine Sci Ctr, Santa Cruz, CA USA Ajinomoto Genet Res Inst, Moscow, Russia Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Plant Biotechnol, Krakow, Poland Univ Puerto Rico, Engn Sci & Mat, Mayaguez, PR USA Univ Regina, Dept Chem & Biochem, Regina, SK, Canada Argonne Natl Lab, Ctr Nanoscale Mat, 9700 S Cass Ave, Argonne, IL 60439 USA Univ Sunshine Coast, Sunshine Coast Mind & Neurosci Thompson Inst, Sippy Downs, Qld, Australia Chinese Peoples Liberat Army Gen Hosp, Dept Gastroenterol & Hepatol, Beijing, Peoples R China Griffith Univ, Griffith Ctr Social & 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Maximilians Univ Munchen, Phys Chem, NanoBioSci, Munich, Germany Bandung Inst Technol, Sch Pharm, Med Chem, Bandung, Indonesia Univ Luxembourg, Life Sci Res Unit, Luxembourg, Luxembourg Lund Univ, Skane Univ Hosp, Dept Gastroenterol, Malmo, Sweden Millennium Hlth, Translat Genet, San Diego, CA USA Aristotle Univ Thessaloniki, Med Dept 2, Clin Res & Evidence Based Med Unit, Thessaloniki, Greece Jan Kochanowski Univ Humanities & Sci, Piotrkow Trybunalski Branch, Dept Psychol, Kielce, Poland McMaster Univ, Engn Phys, Hamilton, ON, Canada Marche Polytech Univ, Dept Agr Food & Environm Sci, Ancona, Italy Kuwait Univ, Fac Med, Microbiol, Kuwait, Kuwait Fujita Hlth Univ, Dept Breast Surg, Toyoake, Aich, Japan North West Reg Spinal Injuries Ctr, Spinal Injuries Ctr, Southport, Merseyside, England Luxembourg Inst Hlth, Competence Ctr Methodol & Stat, Luxembourg, Luxembourg Nestle Inst Hlth Sci SA, Metab Hlth, Ecublens, Vaud, Switzerland Ctr Inflammat Res VIB, Ghent, Belgium Univ Ghent, Dept Biomed Mol Biol, Ghent, Belgium Univ Lisbon, Inst Educ, Curriculo Formacao Prof & Tecnol, Lisbon, Portugal Univ Edinburgh, Ctr Inflammat Res, Edinburgh, Midlothian, Scotland Univ Melbourne, Sch BioSci, Parkville, Vic, Australia Northumbria Univ, Comp & Informat Sci, Newcastle Upon Tyne, Tyne & Wear, England Univ Valencia, Endocrinol, Valencia, Spain INRS, Inst Armand Frappier, Laval, PQ, Canada Univ Laval, INAF, Sch Nutr, Quebec City, PQ, Canada Univ Konstanz, Dept Biol, Constance, Germany Univ Cote dAzur, LAMHESS, Nice, France Scion, Syst Ecol, Christchurch, New Zealand CUNY, Grad Sch Publ Hlth & Hlth Policy, Epidemiol & Biostat, New York, NY 10021 USA Univ Queensland, Sch Dent, Brisbane, Qld, Australia George Inst Global Hlth, Renal & Metab Div, Sydney, NSW, Australia Wuhan Univ, Coll Chem & Mol Sci, Wuhan, Hubei, Peoples R China Griffith Univ, Sch Environm & Sci, Gold Coast, Qld, Australia Univ Minnesota, Radiat Oncol, Minneapolis, MN USA Goethe Univ, Fac Med, Frankfurt, Germany 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Tradit Chinese Med, Inst Interdisciplinary Med Sci, Shanghai, Peoples R China McMaster Univ, Biol, Hamilton, ON, Canada Univ S Florida, Dept Cell Biol Microbiol & Mol Biol, Tampa, FL USA Hacettepe Univ, Inst Canc, Med Oncol, Ankara, Turkey City Univ Hong Kong, Dept Elect Engn, Hong Kong, Peoples R China Natl Taiwan Univ, Dept Entomol, Taipei, Taiwan Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Ecol Secur, Beijing, Peoples R China Florida State Univ, Inst Mol Biophys, Chem & Biochem, Tallahassee, FL USA Peking Univ, Shenzhen Grad Sch, State Key Lab Chem Oncogen, Lab Computat Chem & Drug Design, Shenzhen, Peoples R China Univ Helsinki, Fac Pharm, Div Pharmaceut Chem & Technol, Drug Res Program, Helsinki, Finland Tohoku Univ, Microbial Biotechnol, Sendai, Miyagi, Japan Tianjin Med Univ, Sch Basical Med Sci, Dept Pharmacol, Tianjin, Peoples R China Dana Farber Canc Inst, Biostat & Computat Biol, Boston, MA 02115 USA Natl Hlth Res Inst, Inst Mol & Genom Med, Zhunan, Taiwan Univ Oxford, Physiol Anat & Genet, Oxford, England George Washington Univ, Phys, Washington, DC USA Univ Nebraska, Sch Biol Sci, Lincoln, NE USA Toronto Gen Hosp, Res Inst, Dept Lab Med & Pathobiol, Toronto, ON, Canada Univ Texas Dallas, Biol Sci, Richardson, TX 75083 USA NYU, Dept Chem, New York, NY USA Shandong Univ, Sch Math & Stat, Jinan, Shandong, Peoples R China Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei, Anhui, Peoples R China Purdue Univ, Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA NIBSC, Adv Therapies, Ridge, Herts, England Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Pulm & Crit Care Med, Shanghai, Peoples R China Charite Med Univ Berlin, Dermatol & Allergy, Berlin, Germany Univ Hosp St Etienne, Hematol, St Etienne, France Inland Norway Univ Appl Sci, Inst Biotechnol, Elverum, Norway Univ Jordan, Pediat, Amman, Jordan Inst Pasteur, Mol Mycol Unit, Paris, France Cardiff Univ, Sch Med, Inst Psychol Med & Clin Neurosci, Med Res Council Ctr Neuropsychiat Genet & Genom, Cardiff, S Glam, Wales Zurich Univ Appl Sci, Social Work, Zurich, Switzerland Jawaharlal Nehru Univ, Sch Life Sci, New Delhi, India Univ Burgundy Franche Comte, LE2I, Dijon, France Univ Roehampton, Life Sci, London, England Ghent Univ Hosp, Gen & HPB Surg, Ghent, Belgium Univ Wurzburg, Insect Fungus Symbiosis Lab, Wurzburg, Germany Radboud Univ Nijmegen, Behav Sci Inst, Nijmegen, Netherlands Fraunhofer WKI, Applicat Ctr HOFZET, Hannover, Germany UCL, Struct & Mol Biol, London, England Univ Amsterdam, Dev Psychol, Amsterdam, Netherlands Aalborg Univ, Hlth Sci & Technol, CNAP, SMI, Aalborg, Denmark VA Pittsburgh Healthcare Syst, Ctr Hlth Equity Res & Promot, Pittsburgh, PA USA Cedars Sinai Med Ctr, Neurosurg, Los Angeles, CA 90048 USA Sun Yat Sen Univ, Sch Data & Comp Sci, Guangzhou, Guangdong, Peoples R China Dezhou Univ, Shandong Prov Key Lab Biophys, Guangzhou, Guangdong, Peoples R China Maison Teledetection, Inst Rech Dev, UMR Espace DEv, Montpellier, France Xiamen Univ, Sch Life Sci, Xiamen, Fujian, Peoples R China Nanjing Univ, Sch Med, Jinling Hosp, Natl Clin Res Ctr Kidney Dis,Dept Med Imaging, Nanjing, Jiangsu, Peoples R China Univ Kent, Sch Social Policy Sociol & Social Res, Canterbury, Kent, England Univ Fed Minas Gerais, Infect Dis & Trop Med, Belo Horizonte, MG, Brazil Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, Braga, Portugal Univ Montreal, Biochim & Med Mol, Montreal, PQ, Canada Johns Hopkins Bloomberg Sch Publ Hlth, Epidemiol, Baltimore, MD USA Max Planck Inst Biol Ageing, Metab & Genet Regulat Ageing, Cologne, Germany Univ Swaziland, Hlth Sci, Kwaluseni, Eswatini Queensland Univ Technol, Inst Future Environm, Brisbane, Qld, Australia Ctr Sci Monaco, Dept Biol Med, Monaco, Monaco HELIOS Hosp, Urol, Bad Saarow Pieskow, Germany Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, Braunschweig, Germany Univ Barcelona, Barcelona Ctr Maternal Fetal & Neonatal Med, Fetal i D Fetal Med Res Ctr, IDIBAPS BCNatal,Hosp Clin, Barcelona, Spain Univ Barcelona, Hosp St Joan de Deu, Barcelona, Spain Friedrich Loeffler Inst, Inst Bacterial Infect & Zoonoses, Jena, Germany Charite Med Univ Berlin, Neurol, Berlin, Germany Dublin City Univ, Natl Inst Cellular Biotechnol, Mol Therapeut Canc Ireland, Dublin, Ireland Schoen Clin Roseneck, Prien Am Chiemsee, Germany Univ Med Ctr Hamburg Eppendorf, Inst Sex Res & Forens Psychiat, Hamburg, Germany Nankai Univ, Sch Math Sci, Tianjin, Peoples R China Nankai Univ, LPMC, Tianjin, Peoples R China Univ Oxford, Oncol, Oxford, England Royal Holloway Univ London, Class, Egham, Surrey, England Cornell Univ, Clin Sci, Ithaca, NY USA Univ KwaZulu Natal, Pharmaceut Chem, Westville Campus, Durban, South Africa Royal Coll Surgeons Ireland, Med, Dublin, Ireland Univ Oslo, Dept Immunol, Oslo, Norway Bermuda Inst Ocean Sci, Marine Nitrogen Cycling Lab, St Georges, Bermuda Kanazawa Univ, Inst Liberal Arts & Sci, Kanazawa, Ishikawa, Japan World Hlth Org Reg Off Africa, Brazzaville, Rep Congo Univ Hosp BesanCon, Infect Control Dept, Besancon, France Galapagos NV, Clin Dev, Mechelen, Belgium Univ Tasmania, Integrated Marine Observing Syst, Hobart, Tas, Australia Georg August Univ Gottingen, Albrecht von Haller Inst Plant Sci, Dept Systemat Biodivers & Evolut Plants, Gottingen, Germany Univ Occupat & Environm Hlth, Dept Psychiat, Fukuoka, Fukuoka, Japan IMDEA Food, Program Precis Nutr & Aging, Madrid, Spain Radboud Univ Nijmegen, Med Sch, IQHealthcare, Nijmegen, Netherlands Maastricht Univ, Dept Cardiovasc Surg, Maastricht, Netherlands German Diabet Ctr, Inst Clin Biochem & Pathobiochem, Dusseldorf, Germany Juntendo Univ, Grad Sch Med, Dept Radiol, Tokyo, Japan Deakin Univ, Sch Informat Technol, Melbourne, Vic, Australia Max Planck Inst Eusenforschung, Dept Interface Chem & Surface Sci, Dusseldorf, Germany Edge Hill Univ, Dept Psychol, Ormskirk, England Aga Khan Univ, Psychiat, Karachi, Pakistan KRIBB, Korean Bioinformat Ctr, Seoul, South Korea Cardinal Hlth Specialty Solut, Hlth Econ & Outcomes Res, Dallas, TX USA Klinikum Univ Munchen, Div Clin Pharmacol, Munich, Germany Univ Pittsburgh, Neurol Surg, Pittsburgh, PA USA Rhein Westfal TH Aachen, Dept Child & Adolescent Psychiat Psychosomat & Ps, Aachen, Germany Univ Copenhagen, Inst Mol & Cellular Biol, Copenhagen, Denmark St Jude Childrens Res Hosp, Struct Biol, 332 N Lauderdale St, Memphis, TN 38105 USA Royal Shrewsbury Hosp, Colorectal Surg, Shrewsbury, Salop, England Univ Nottingham, Fac Med & Hlth Sci, Nottingham, England Karolinska Inst, Dept Physiol & Pharmacol, Solna, Sweden Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Jilin, Jilin, Peoples R China Univ British Columbia, Pediat, Vancouver, BC, Canada Chinese Acad Agr Sci, State Key Lab Cotton Biol, Res Base Anyang Inst Technol, Cotton Germplasm Resources,Inst Cotton Res, Beijing, Peoples R China Chinese Univ Hong Kong, Anaesthesia & Intens Care, Hong Kong, Peoples R China Univ Macau, ICMS, Zhuhai, Guangdong, Peoples R China North China Elect Power Univ, Sch Renewable Energy, Beijing, Peoples R China Justus Liegbig Univ, Dept Internal Med, Giessen, Germany Aarhus Univ, Biosci, Aarhus, Denmark Univ Dublin, Trinity Coll Dublin, Irish Longitudinal Study Ageing TILDA, Dublin, Ireland Univ Groningen, Univ Med Ctr Groningen, Hematol, Groningen, Netherlands Vrije Univ Amsterdam Med Ctr, Child Neurol, Amsterdam, Netherlands EBI, EMBL, Cambridge, England Max Planck Inst Marine Microbiol, HGF MPG Joint Res Grp Deep Sea Ecol & Technol, Bremen, Germany Max Planck Inst Human Dev, Ctr Adapt Rat, Berlin, Germany King Faisal Univ, Math, Al Hufuf, Saudi Arabia Griffith Univ, Sch Nursing & Midwifery, Gold Coast, Qld, Australia Iowa State Univ, Roy J Carver Dept Biochemsitry Biophys & Mol Biol, Ames, IA USA Delft Univ Technol, Fac Mech Maritime & Mat Engn, Engn Thermodynam Proc & Energy Dept, Leeghwaterstr 39, NL-2628 CB Delft, Netherlands Univ Nebraska Med Ctr, Coll Allied Hlth Profess, Cytotechnol Educ, Omaha, NE USA Shinko Mem Hosp, Dept Cardiovasc Med, Kobe, Hyogo, Japan Imperial Coll London, Mat, London, England Tech Univ Munich, Dept Surg, Munich, Germany Chinese Acad Agr Sci, Res Inst Pomol, Minist Agr, Lab Qual & Safety Risk Assessment Fruit Xingcheng, Shenyang, Liaoning, Peoples R China James Madison Univ, Commun Sci & Disorders, Harrisonburg, VA 22807 USA Univ Hosp Ulm, Inst Orthopaed Res & Biomech, Ulm, Germany Univ Essex, Sch Hlth & Social Care, Colchester, Essex, England Alpha Altis, Res Serv, Nottingham, England Erasmus MC, Med Oncol, Rotterdam, Netherlands Fed Univ Oye, Dept Ind Chem, Ekiti, Nigeria Duke Univ, Med Ctr, Cell Biol, Durham, NC USA Univ Oxford, Nuffield Dept Clin Neurosci, Oxford, England Univ Manchester, Canc Res UK Manchester Inst, Manchester, Lancs, England Helsinki Univ Hosp, Childrens Hosp, Helsinki, Finland Univ Aveiro, CESAM Ctr Environm & Marine Studies, Dept Biol, Aveiro, Portugal Univ Botswana, Psychol, Gaborone, Botswana Univ Fed Bahia, Nursing Sch, Salvador, BA, Brazil Queen Mary Univ London, Biol & Expt Psychol, London, England Natl Univ Pharm, Med Chem Dept, Kharkov, Ukraine Univ Bolton, Dept Educ & Psychol, Bolton, England La Trobe Univ, Dept Chem & Phys, Melbourne, Vic, Australia Gen Hosp Northern Theater Command, Dept Gastroenterol, Shenyang, Liaoning, Peoples R China Doctors Hosp, Dept Nephrol, Athens, Greece Univ Hosp Essen, Pediat 3, Essen, Germany Imperial Coll London, Infect Dis Epidemiol, London, England Sorbonne Univ, Dept Psychiat, Paris, France UNSW Sydney, Educ, Sydney, NSW, Australia Stanford Univ, Dept Psychiat & Behav Sci, Palo Alto, CA 94304 USA Hannover Med Sch, Clin Laryngol Rhinol & Otol, Hannover, Germany Curtin Univ, Ctr Aboriginal Studies, Perth, WA, Australia Iran Univ Sci & Technol, Biomed Engn Dept, Tehran, Iran Univ Calif San Francisco, Anesthesiol, San Francisco, CA 94143 USA Khalifa Univ Sci & Technol, Mech Engn, Abu Dhabi, U Arab Emirates Univ Florida, Hort Sci, Gainesville, FL USA James Cook Univ, Australian Inst Trop Hlth & Med, Ctr Biodiscovery & Mol Dev Therapeut, Cairns, Qld, Australia Univ Porto, Fac Med, CINTESIS, Porto, Portugal Shaoxing Peoples Hosp, Med Res Ctr, Shaoxing, Zhejiang, Peoples R China NIH, Dept Transfus Med, Bethesda, MD 20892 USA AIIMS, Dept Biotechnol, New Delhi, India Univ Ottawa, Biochem Microbiol & Immunol, Ottawa, ON, Canada Univ Oslo, Inst Clin Med, Div Mental Hlth & Addict, Oslo, Norway Univ Groningen, Univ Med Ctr Groningen, Dept Radiol, Groningen, Netherlands Univ Hong Kong, Sch Nursing, Hong Kong, Peoples R China Tokyo Med Univ, Ibaraki Med Ctr, Urol, Tokyo, Japan Univ Hosp Zurich, Dept Radiat Oncol, Zurich, Switzerland Univ Maryland, Inst Human Virol, Div Immunotherapy, Baltimore, MD 21201 USA Univ Maryland, Dept Surg, Baltimore, MD 21201 USA Stellenbosch Univ, Fac Med & Hlth Sci, Div Mol Biol & Human Genet, Stellenbosch, South Africa China Agr Univ, Coll Biol Sci, Beijing, Peoples R China Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka, Japan Univ Washington, Biochem, Seattle, WA 98195 USA Natl Res Council Italy, Inst Biosci & BioResources, Naples, Italy Univ Lyon, Phys, Lyon, France Univ Basel, Fac Psychol, Ctr Social Psychol, Basel, Switzerland Queen Mary Univ London, Barts Canc Inst, Ctr Mol Oncol, London, England EBI, EMBL, Samples Phenotypes & Ontol Team, Cambridge, England Charles Sturt Univ, Fac Arts & Educ, Bathurst, NSW, Australia Shandong Univ, Helmholtz Inst Biotechnol, Sch Life Sci, State Key Lab Microbial Technol, Jinan, Shandong, Peoples R China Shantou Univ, Dept Biol, Shantou, Guangdong, Peoples R China Shanxi Univ, Inst Biomed Sci, Taiyuan, Shanxi, Peoples R China St Jude Childrens Res Hosp, Computat Biol, 332 N Lauderdale St, Memphis, TN 38105 USA Harbin Med Univ, Coll Bioinformat Sci & Technol, Harbin, Heilongjiang, Peoples R China NIH, Radiol & Imaging Sci, Bldg 10, Bethesda, MD 20892 USA Georgia Inst Technol, Dept Biol Sci, Atlanta, GA 30332 USA XtalPi Inc, Cambridge, MA USA Consejo Nacl Invest Cient & Tecn, Partner Inst Max Planck Soc, Inst Invest Biomed Buenos Aires IBioBA, Bioinformat, Buenos Aires, DF, Argentina Univ Sydney, Save Sight Inst, Sydney, NSW, Australia Univ South Australia, Canc Res Inst, Australian Ctr Precis Hlth, Adelaide, SA, Australia Jinan Univ, Inst Life & Hlth Engn, Guangdong Higher Educ Inst, Key Lab Funct Prot Res, Guangzhou, Guangdong, Peoples R China Univ Texas Hlth Sci Ctr Houston, Epidemiol Human Genet & Environm Sci, Houston, TX 77030 USA Weill Cornell Med, Dept Microbiol & Immunol, New York, NY USA Guangdong Inst Appl Biol Resources, Biotechnol Lab, Guangzhou, Guangdong, Peoples R China Shandong Normal Univ, Coll Life Sci, Jinan, Shandong, Peoples R China Shandong Univ, Life Sci Dept, Jinan, Shandong, Peoples R China South China Agr Univ, Integrat Microbiol Res Ctr, Guangzhou, Guangdong, Peoples R China Liaoning Acad Agr Sci, Crop Mol Improving Lab, Shenyang, Liaoning, Peoples R China Lawson Hlth Res Inst, Med Biophys, London, ON, Canada Univ Melbourne, Infrastruct Engn, Parkville, Vic, Australia Univ Canberra, Fac Hlth, Canberra, ACT, Australia Univ Cambridge, MRC Cognit & Brain Sci Unit, Cambridge, England Emory Univ, Biostat & Bioinformat, Atlanta, GA 30322 USA Johns Hopkins Sch Med, Anesthesiol & Crit Care Med, Baltimore, MD USA Nottingham Trent Univ, Sch Anim Rural & Environm Sci, Nottingham, England Univ Exeter, Biosci, Exeter, Devon, England Hillingdon Hosp NHS Fdn Trust, London, England Univ Glasgow, MRC CSO Social & Publ Hlth Sci Unit, Glasgow, Lanark, Scotland Natl & Kapodistrian Univ Athens, Evaggelismos Athens Hosp, ICU, Athens, Greece Univ Newcastle, Biol Sci, Callaghan, NSW, Australia Coventry Univ, Fac Hlth & Life Sci, Ctr Innovat Res Life Course, Coventry, W Midlands, England Lausanne Univ Hosp, Serv Endocrinol Diabet & Metab, Lausanne, Switzerland Charles Sturt Univ, Sch Community Hlth, Bathurst, NSW, Australia Queens Univ Belfast, Inst Global Food Secur, Belfast, Antrim, North Ireland Natl Univ Singapore, Inst Policy Studies, Singapore, Singapore Univ Penn, Intitute Med & Engn, Philadelphia, PA 19104 USA Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA Univ Michigan, EECS, Ann Arbor, MI 48109 USA Univ British Columbia, Ctr Blood Res, Vancouver, BC, Canada UiT Arctic Univ Norway, Dept Hlth & Care Sci, Fac Hlth Sci, Tromso, Norway Hosp Clin Porto Alegre, Physiotherapy, Porto Alegre, RS, Brazil Univ Paris 05, Med Sch, Paris, France Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China Univ Ghent, Expt Clin & Hlth Psychol, Ghent, Belgium Indian Inst Adv Res, Bioinformat & Struct Biol, Gandhinagar, Gujart, India Bambino Ges Childrens Res Hosp, Lab Mol Med, Rome, Italy Heidelberg Univ, Ctr Infect Dis Parasitol, Heidelberg, Germany Stanford Univ, Elect Engn, Palo Alto, CA 94304 USA Univ Cadiz, Biol, Andalucia, Spain Mansoura Univ Hosp, Gen Surg, Mansoura, Egypt Inst Pasteur, Virol Pole, Dakar, Senegal Cardiff Univ, Div Canc & Genet, Cardiff, S Glam, Wales Ctr Expertise & Biol Diagnost Cameroon, Food Safety & Environm Microbiol, Yaounde, Cameroon Swiss Fed Labs Mat Sci & Technol, Lab Thin Films & Photovolta, Dubendorf, Switzerland Assiut Univ, Assiut Urol & Nephrol Hosp, Fac Med, Assiut, Egypt UCL, GEE, London, England UCL, IHA, London, England Univ Derby, Univ Derby Online Learning, Derby, England SUNY Stony Brook, Family Populat & Prevent Med, Stony Brook, NY 11794 USA Walter & Eliza Hall Inst Med Res, Mol Med Div, Melbourne, Vic, Australia Newcastle Univ, Northern Inst Canc Res, Newcastle Upon Tyne, Tyne & Wear, England German Ctr Neurodegenerat Dis, Clin Dementia Res, Bonn, Germany Sorbonne Univ, CNRS, UMR 7144, Stn Biol, Paris, France Univ Barcelona, Odontoestomatol, Barcelona, Spain Janelia Res Campus, Comp Sci, Ashburn, VA USA Univ Oxford, Ctr Trop Med & Global Hlth, Oxford, England Univ Bern, ARTORG Ctr Biomed Engn Res, Bern, Switzerland Australian Natl Univ, Eccles Inst Neurosci, John Curtain Sch Med Res, Canberra, ACT, Australia John Innes Ctr, Metab Biol, Norwich, Norfolk, England USDA ARS, Genom & Bioinformat Res Unit, Raleigh, NC 27695 USA Med Univ Graz, Inst Med Informat Stat & Documentat, Holzinger Grp, Graz, Austria Ajou Univ, Pharm, Suwon, South Korea City Univ Hong Kong, Sch Energy & Environm, Hong Kong, Peoples R China Univ British Columbia, Sch Kinseiol, Vancouver, BC, Canada Univ Copenhagen, Marine Biol Sect, Dept Biol, Copenhagen, Denmark Univ Vienna, Dept Commun, Vienna, Austria Univ Dundee, Sch Social Sci, Dundee, Scotland Tech Univ Dresden, Inst Bot, Dresden, Germany Univ Oxford, Div Struct Biol, Oxford, England Natl Univ Hlth Syst, Med, Singapore, Singapore Univ Canterbury, Sch Biol Sci, Christchurch, New Zealand Univ Hosp Southern Denmark, Focused Res Unit Mol Diagnost & Clin Res, Odense, Denmark Univ Oxford, Primary Care Hlth Sci, Oxford, England Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA Adnan Menderes Univ Aydin, Fac Nursing, Dept Publ Hlth Nursing, Aydin, Turkey Oasi Res Inst IRCCS, Dept Neurol IC, Troina, Italy Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA Kings Coll London, Kings Ctr Mil Hlth Res, London, England LSHTM, Dept Infect Dis Epidemiol, London, England Leibniz Univ Hannover, BMWZ Organ Chem, Hannover, Germany Xi An Jiao Tong Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Xian, Shaanxi, Peoples R China Univ South Australia, Sch Pharm & Med Sci, Adelaide, SA, Australia Univ Fed Santa Catarina, Dept Phys Educ, Florianopolis, SC, Brazil Southern Med Univ, Nanfang Hosp, Dept Oncol, Guangzhou, Guangdong, Peoples R China Stanford Univ, Hansen Expt Phys Lab, Palo Alto, CA 94304 USA Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Inst Translat Med, Shenzhen, Guangdong, Peoples R China Univ Hong Kong, Dept Stat & Actuarial Sci, Hong Kong, Peoples R China UCL, Dept Mech Engn, London, England ASTAR, Singapore Immunol Network, Lab Microbial Immun, Singapore, Singapore Cent South Univ, State Key Lab Powder Met, Changsha, Hunan, Peoples R China Univ Aberdeen, Inst Appl Hlth Sci, Aberdeen, Scotland Univ Bridgeport, Biomed Engn, Bridgeport, CT 06601 USA Texas Tech Univ, Hlth Sci Ctr, Pharmaceut Sci, Lubbock, TX 79430 USA Univ Montana, Ecosyst & Conservat Sci, Missoula, MT 59812 USA Univ Goettingen, Dept Syst Neurosci, Gottingen, Germany NHLBI, Lab Syst Genet, Bldg 10, Bethesda, MD 20892 USA Cleveland Clin, Lou Ruvo Ctr Brain Hlth, Imaging, Las Vegas, NV USA Flinders Univ S Australia, Coll Nursing & Hlth Sci, Nutr & Dietet, Adelaide, SA, Australia Univ Padua, Dept Math, Padua, Italy Lund Univ, Fac Law, Lund, Sweden Univ Gothenburg, Dept Microbiol & Immunol, Gothenburg, Sweden NARO, Kachwekano Zardi, Entebbe, Uganda Natl Yunlin Univ Sci & Technol, Bachelor Program Interdisciplinary Studies, Touliu, Yunlin, Taiwan Aarhus Univ, Dept Biomed, Danish Res Inst Translat Neurosci DANDRITE, Aarhus, Denmark Eduardo Mondlane Univ, Math & Comp Sci, Maputo, Mozambique Univ Bern, Dept Old Age Psychiat & Psychotherapy, Bern, Switzerland RAS, Inst Cytol, Lab Cytol Unicellular Organisms, St Petersburg, Russia Beijing Inst Technol, Sch Chem & Chem Engn, Beijing, Peoples R China Univ Queensland, Queensland Alliance Agr & Food Innovat, Brisbane, Qld, Australia Fraunhofer Inst Toxicol & Expt Med ITEM, Inhalat Toxicol, Hannover, Germany Univ Hong Kong, Publ Hlth, Hong Kong, Peoples R China Univ Hlth Network, Anesthesia & Pain Med, Toronto, ON, Canada Univ Toronto, Toronto, ON, Canada Univ Bath, Dept Hlth, Bath, Avon, England Univ Copenhagen, Computat & RNA Biol, Copenhagen, Denmark Fisheries & Oceans Canada, Bedford Inst Oceanog, Dartmouth, NS, Canada Goethe Univ, CEF MC, BMLS, Phys Biol, Frankfurt, Germany Albert Einstein Coll Med, Anat & Struct Biol, New York, NY USA Queensland Govt, Dept Environm & Sci, Brisbane, Qld, Australia Uppsala Univ, Vasc Surg Sect, Dept Surg Sci, Uppsala, Sweden Childrens Canc Hosp, Res, Cairo, Egypt Leibniz Inst Nat Prod Res & Infect Biol, Bio Pilot Plant, Jena, Germany Duy Tan Univ, Inst Res & Dev, Da Nang, Vietnam Univ Helsinki, Helsinki Inst Life Sci HiLIFE, Helsinki, Finland Univ Queensland, Australian Inst Bioengn & Nanotechnol, Brisbane, Qld, Australia George Inst Global Hlth, Sydney, NSW, Australia Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld, Australia Dezhou Univ, Coll Phys & Elect Informat, Shandong Prov Key Lab Biophys, Dezhou, Peoples R China Henan Agr Univ, Coll Life Sci, Zhengzhou, Henan, Peoples R China Univ Tokyo, Publ Hlth, Tokyo, Japan Sun Yat Sen Univ, Affiliated Hosp 1, Guangzhou, Guangdong, Peoples R China Univ Illinois, Dept Med, Chicago, IL USA Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China Minist Hlth, Key Lab Neonatal Dis, Shanghai, Peoples R China Covenant Univ, Dept Phys, Ota, Nigeria Prince Sattam Bin Abdulaziz Univ, Dept Phys Therapy & Hlth Rehabil, Al Kharj, Saudi Arabia Lund Univ, Cognit Sci, Malmo, Sweden Natl Open Univ Nigeria, Dept Publ & Environm Hlth, Abuja, Nigeria Peking Univ, Sch Publ Hlth, Dept Lab Sci & Technol, Beijing, Peoples R China Univ Sydney, Sch Publ Hlth, Menzies Ctr Hlth Policy, Sydney, NSW, Australia Univ Auckland, Dept Elect & Comp Engn, Auckland, New Zealand Beijing Univ Chinese Med, Res Ctr TCM Informat Engn, Beijing, Peoples R China Osped Niguarda Ca Granda, Cardiac Surg, Milan, Italy Univ Vet Med, Clin Horses, Hannover, Germany Harbin Med Univ, Lab Med Genet, Harbin, Heilongjiang, Peoples R China Univ Saskatchewan, Dept Psychol, Saskatoon, SK, Canada Univ Coimbra, Ctr Studies Geog & Spatial Planning CEGOT, Coimbra, Portugal Univ Groningen, Univ Med Ctr Groningen, Epidemiol, Groningen, Netherlands South Cent High Specialty Hosp, Dept Neurol & Neurosurg, Pemex, Mexico Shandong Agr Univ, Coll Informat Sci & Engn, Tai An, Shandong, Peoples R China Curtin Univ, Natl Drug Res Inst, Perth, WA, Australia Wageningen Bioveterinary Res, Bacteriol & Epidemiol, Lelystad, Netherlands Guangdong Second Prov Gen Hosp, Dept Rheumatol & Immunol, Guangzhou, Guangdong, Peoples R China Erasmus MC, Biomed Rngineering, Rotterdam, Netherlands Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Hiroshima, Japan Univ Iceland, Sch Hlth Sci, Reykjavik, Iceland Ohio State Univ, Mat Sci & Engn, Columbus, OH 43210 USA Kathmandu Univ, Sch Med Sci, Dept Physiotherapy, Dhulikhel, Nepal Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia Fraunhofer MEVIS, Image Guided Therapies, Bremen, Germany Natl Univ Hlth Syst, Haematol Oncol, Singapore, Singapore Sun Yat Sen Univ, Canc Ctr, Breast Oncol, Guangzhou, Guangdong, Peoples R China Med Coll Wisconsin, Pharmacol & Toxicol, Wauwatosa, WI USA Queensland Univ Technol, Sci & Engn Fac, Sch Chem Phys & Mech Engn, Brisbane, Qld, Australia Univ Turin, Dept Mol Biotechnol & Hlth Sci, Turin, Italy Univ Tehran Med Sci, Sch Rehabil, Physiotherapy Dept, Tehran, Iran Univ Helsinki, Dept Forest Sci, Helsinki, Finland Univ Messina, Human Pathol, Messina, Italy AO Papardo Hosp Messina, Messina, Italy Univ Ibadan, Coll Med, Inst Child Hlth, Ibadan, Nigeria King Faisal Univ, Coll Med, Fac Ophthalmol, Al Hasa, Saudi Arabia Univ Stirling, Inst Social Mkt, Stirling, Scotland Saveh Univ Med Sci, Social Determinants Hlth Res Ctr, Saveh, Iran Gakujutsu Shien Co Ltd, Tokyo, Japan Chinese Acad Sci, Inst Geochem, Guiyang, Guizhou, Peoples R China Univ Plymouth, Med Sch, Plymouth, Devon, England CHU Toulouse, Immunol, Toulouse, France Azorean Biodivers Grp, Ctr Ecol Evolut & Environm Changes, Azores, Portugal Univ Acores, Azores, Portugal RIKEN, Ctr Integrat Med Sci, Yokohama, Kanagawa, Japan Peking Univ, Sch Publ Hlth, Dept Global Hlth, Beijing, Peoples R China Chang Gung Univ, Chang Gung Mem Hosp, Dept Neurol, Linkou Med Ctr, Taoyuan, Taiwan Chang Gung Univ, Coll Med, Taoyuan, Taiwan Univ Malawi, Coll Med, Biomed Sci Dept, Blantyre, Malawi Univ Malawi, Coll Med, Pharm Dept, Blantyre, Malawi Bioself Commun, Biocurat, Marseille, France Peking Univ, Hosp 3, Dept Neurol, Beijing, Peoples R China Ahmadu Bello Univ, Fac Basic Clin Sci, Coll Hlth Sci, Dept Pathol, Zaria, Nigeria Dalhousie Univ, Dept Anesthesia Pain Management & Perioperat Med, Halifax, NS, Canada VisMederi Srl, Siena, Italy UCL, Canc Res UK, London, England UCL, UCL Canc Trials Ctr, London, England Univ Ottawa, Family Med, Ottawa, ON, Canada China Agr Univ, Coll Engn, Beijing, Peoples R China Leiden Univ, Leiden Acad Ctr Drug Res, Div Drug Discovery & Safety, Leiden, Netherlands Sun Yat Sen Univ, Affiliated Hosp 1, Dept Intervent Radiol, Guangzhou, Guangdong, Peoples R China Amer Univ Beirut, Med Ctr, Infect Dis, Beirut, Lebanon Sheffield Hallam Univ, Dept Social Work Social Care & Community Studies, Sheffield, S Yorkshire, England Mechnikov Res Inst Vaccines & Sera, Viral Hepatitis, Moscow, Russia Univ Ottawa, Pediat, Ottawa, ON, Canada Vreden Russian Res Inst Traumatol & Orthopaed, Dept Wound Infect Treatment & Prevent, St Petersburg, Russia Hangzhou Ctr Dis Control & Prevent, Dept TB Control & Prevent, Hangzhou, Zhejiang, Peoples R China Kaohsiung Med Univ, Dept Biotechnol, Kaohsiung, Taiwan Zoetis, Diagnost, Kalamazoo, MI USA Aintree Univ Hosp NHS Fdn Trust, Head & Neck Oncol Res, Liverpool, Merseyside, England Wrightington Hosp, Trauma & Orthopaed, Manchester, Lancs, England Loyola Univ, Med Ctr, Dept Psychiat, 2160 S 1st Ave, Maywood, IL 60153 USA Atkins Vet Serv, Microbiol, Calgary, AB, Canada Univ Porto, FADEUP, CIAFEL, Porto, Portugal Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore, Singapore Kangwon Natl Univ, Coll Biotechnol & Biosci, Dept Food Sci & Biotechnol, Chunchon, South Korea Kakatiya Med Coll, Internal Med, Warangal, Telangana, India Univ Antioquia, Vet Med Sch, CIBAV Res Grp, Medellin, Colombia IISER, Dept Phys, Soft & Act Matter Grp, Tirupati 517507, Andhra Pradesh, India Univ Rosario, Sch Med & Hlth, Ctr Studies Phys Activ Measurements, Bogota, Colombia Univ Hosp Essen, Cardiol & Vasc Med, Essen, Germany Univ Hosp Basel, Endocrinol, Basel, Switzerland Univ Tubingen, Inst Med Genet & Appl Genom, Tubingen, Germany Univ Hosp Munster, Div Gen Internal Med Nephrol & Rheumatolog, Dept Med D, Munster, Germany Univ Kentucky, Dept Nephrol, Lexington, KY USA Univ Freiburg, Dept Anaesthesiol & Crit Care, Med Ctr, Freiburg, Germany Univ Calif Irvine, Dept Med, Orange, CA 92668 USA Univ Hosp Leuven, Dept Urol, Leuven, Belgium Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China Univ Florida, Orthopaed & Rehabil, Gainesville, FL USA Chongqing Med Univ, Affiliated Hosp 1, Chongqing Key Lab Mol Oncol & Epigenet, Chongqing, Peoples R China Tsinghua Univ, Dept Chem Engn, Beijing, Peoples R China Yonsei Univ, Coll Med, Dept Pharmacol, Seoul, South Korea Childrens Hosp Kings Daughters, Eastern Virginia Med Sch, Dept Pediat, Norfolk, VA USA China Three Gorges Univ, Coll Sci, Dept Math, Yichang, Peoples R China Xiangtan Univ, Coll Informat Engn, Xiangtan, Hunan, Peoples R China Univ Hlth Network, Mood Disorders & Psychopharmacol, Toronto, ON, Canada Sao Paulo State Univ UNESP, Dept Anim Sci, Sao Paulo, Brazil Sao Paulo State Univ, Vet Clin, Sao Paulo, Brazil
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- 2019
- Full Text
- View/download PDF
24. Report from the OECI Oncology days 2014
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F Paparo, F Lemare, Darius Razavi, P De Paoli, PA Cadariu, F. De Lorenzo, Johannes Haybaeck, GA Rollandi, Gunnar Folprecht, Giovanni Bussolati, Peter Riegman, JA López Guerrero, Giorgio Stanta, T Ciuleanu, Marco A. Pierotti, Claudio Lombardo, József Lövey, S Zupo, R Buiga, W.H. van Harten, Mauro Truini, A Bagg, M Dono, Karl-Friedrich Becker, Gerald Hoefler, Mahasti Saghatchian, N. Goedbloed, G Weiner, Health Technology & Services Research, Faculty of Behavioural, Management and Social Sciences, van Harten, W, Stanta, Giorgio, Bussolati, G, Riegman, P, Hoefler, G, Becker, K, Folprecht, G, Truini, M, Haybaeck, J, Buiga, R, Dono, M, Bagg, A, López Guerrero, J, Zupo, S, Lemare, F, de Lorenzo, F, Goedbloed, N, Razavi, D, Lövey, J, Cadariu, P, Rollandi, G, Paparo, F, Pierotti, M, Ciuleanu, T, De Paoli, P, Weiner, G, Saghatchian, M, Lombardo, C., and Pathology
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Oncology ,Cancer Research ,medicine.medical_specialty ,Designation ,organisation ,comprehensive cancer centres ,designation ,accreditation ,personalized medicine ,Comprehensive cancer centres ,Accreditation ,Environnement et pollution ,Clinical decision making ,SDG 3 - Good Health and Well-being ,Internal medicine ,IR-97320 ,medicine ,Tumor biopsy ,METIS-311886 ,business.industry ,comprehensive cancer centre ,Cancer survival ,Conference Report ,Personalized medicine ,Health care delivery ,Cancérologie ,Organisation ,Cancer Radiotherapy ,Day hospital ,business - Abstract
The 2014 OECI Oncology Days was held at the 'Prof. Dr. Ion Chiricuta' Oncology Institute in Cluj, Romania, from 12 to 13 June. The focus of this year's gathering was on developments in personalised medicine and other treatment advances which have made the cost of cancer care too high for many regions throughout Europe., SCOPUS: ar.j, info:eu-repo/semantics/published
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- 2014
25. miR-15 and miR-16 induce apoptosis by targeting BCL2
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Rami I. Aqeilan, George A. Calin, Hansjuerg Alder, Manuela Ferracin, Laura Z. Rassenti, Massimo Negrini, Muller Fabbri, Carlo M. Croce, Marilena V. Iorio, Simona Zupo, Amelia Cimmino, Chang Gong Liu, Masayoshi Shimizu, Sylwia E. Wojcik, Mariella Dono, Stefano Volinia, Thomas J. Kipps, Cimmino A., Calin G. A., Fabbri M., Iorio M. V., Ferracin M., Shimizu M., Wojcik S. E., Aqeilan R. I., Zupo S., Dono M., Rassenti L., Alder H., Volinia S., Liu C. G., Kipps T. J., Negrini M., and Croce C. M.
- Subjects
Transcription, Genetic ,Chronic lymphocytic leukemia ,translation ,Apoptosis ,Biology ,NO ,immune system diseases ,hemic and lymphatic diseases ,microRNA ,medicine ,Humans ,B-cell lymphoma ,neoplasms ,Cell Proliferation ,Regulation of gene expression ,Multidisciplinary ,microRNAs ,leukemia ,Cell growth ,Gene Expression Regulation, Leukemic ,Apoptosi ,MicroRNA ,Leukemia, Lymphocytic, Chronic ,Biological Sciences ,medicine.disease ,Leukemia, Lymphocytic, Chronic, B-Cell ,Leukemia ,MicroRNAs ,Proto-Oncogene Proteins c-bcl-2 ,Cell culture ,Immunology ,Cancer research ,biological phenomena, cell phenomena, and immunity ,Human - Abstract
Chronic lymphocytic leukemia (CLL) is the most common human leukemia and is characterized by predominantly nondividing malignant B cells overexpressing the antiapoptotic B cell lymphoma 2 (Bcl2) protein. miR-15a and miR-16-1 are deleted or down-regulated in the majority of CLLs. Here, we demonstrate that miR-15a and miR-16-1 expression is inversely correlated to Bcl2 expression in CLL and that both microRNAs negatively regulate Bcl2 at a posttranscriptional level. BCL2 repression by these microRNAs induces apoptopsis in a leukemic cell line model. Therefore, miR-15 and miR-16 are natural antisense Bcl2 interactors that could be used for therapy of Bcl2-overexpressing tumors.
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- 2005
26. An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
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Brian Meloon, Mariella Dono, Hansjuerg Alder, Florencia Bullrich, Cinzia Sevignani, Masayoshi Shimizu, George A. Calin, Manuela Ferracin, Simona Zupo, Chang Gong Liu, Carlo M. Croce, Nir Gamliel, Calin Dan Dumitru, Massimo Negrini, LIU C.G., CALIN G.A., MELOON B., GAMLIEL N., SEVIGNANI C., FERRACIN M., DUMITRU C.D., SHIMIZU M., ZUPO S., DONO M., ALDER H., BULLRICH F., NEGRINI M., and CROCE C.M.
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Adult ,Macrophage ,Reproducibility of Result ,Biology ,Genome ,Sensitivity and Specificity ,Mice ,Fetus ,Neoplasms ,microRNA ,Animals ,Humans ,Fetu ,Gene ,Cells, Cultured ,Oligonucleotide Array Sequence Analysis ,Genetics ,Multidisciplinary ,Oligonucleotide ,Animal ,Genome, Human ,Oligonucleotide Array Sequence Analysi ,Reverse Transcriptase Polymerase Chain Reaction ,Macrophages ,Gene Expression Profiling ,Reproducibility of Results ,MicroRNA ,Genomics ,Biological Sciences ,Non-coding RNA ,Blotting, Northern ,Gene expression profiling ,MicroRNAs ,Hela Cell ,Organ Specificity ,Genomic ,Neoplasm ,Human genome ,DNA microarray ,Oligonucleotide Probes ,Oligonucleotide Probe ,HeLa Cells ,Human - Abstract
We performed cytogenetic and molecular studies on an adult patient with refractory anemia with an excess of blasts with an add(11)(p15). Multicolor fluorescence in situ hybridization (FISH) identified the extra material on 11p as belonging to chromosome 15. Metaphase FISH with probes for chromosomes 5, 11, and 15 revealed a complex four-break rearrangement. Clone RP5-1173K1, containing exons 10-20 of the NUP98 gene, gave three fluorescence signals on the normal 11, the der(5), and the der(15). 3'-RACE-PCR identified an in-frame fusion between NUP98 and NSD1, which was confirmed by RT-PCR. Two different spliced forms, that is, NUP98 exon 11/NSD1 exon 6 and NUP98 exon 12/NSD1 exon 6, were detected. The reciprocal NSD1/NUP98 was not found. A dual-color experiment with RP5-1173K1 and CTC-549A4, spanning the entire NSD1 gene, indicated an insertion of NUP98 into the NSD1 locus. This is the first report of an adult with myelodysplastic syndrome (MDS) harboring an NUP98/NSD1 fusion resulting from insertion of 5'-NUP98 into the NSD1/5q35 locus.
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- 2004
27. Streamlining the diagnostic pathway for Lynch syndrome in colorectal cancer patients: a 10-year experience in a single Italian Cancer Center.
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Puccini A, Nardin S, Trevisan L, Lastraioli S, Gismondi V, Ricciotti I, Damiani A, Bregni G, Murialdo R, Pastorino A, Martelli V, Gandini A, Mastracci L, Varesco L, Dono M, Battistuzzi L, Grillo F, and Sciallero S
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- Humans, Female, Male, Middle Aged, Italy epidemiology, Aged, Colorectal Neoplasms diagnosis, Colorectal Neoplasms genetics, Colorectal Neoplasms epidemiology, Proto-Oncogene Proteins B-raf genetics, Adult, DNA Methylation, Genetic Counseling, Mutation, Early Detection of Cancer methods, Early Detection of Cancer statistics & numerical data, Retrospective Studies, Follow-Up Studies, Colorectal Neoplasms, Hereditary Nonpolyposis diagnosis, Colorectal Neoplasms, Hereditary Nonpolyposis genetics, Genetic Testing methods, DNA Mismatch Repair genetics, MutL Protein Homolog 1 genetics
- Abstract
Background: Universal screening of colorectal cancer (CRC) patients for Lynch syndrome (LS) through MisMatch Repair (MMR) testing is recommended. BRAF V600E mutation and/or MLH1 promoter methylation (Reflex Testing, RefT)generally rule out LS in MLH1-deficient (dMLH1) patients. We estimated the impact of RefTon genetic counseling (GC) and on the diagnostic yield of genetic testing (GT)., Methods: Overall, 3199 CRC patients were referred to our center between 2011 and 2021. Patients referred until January 2019 (n=2536) underwent universal MMR testing and were termed 'Cohort A'; among patients after February 2019 (n=663), 'Cohort B', RefT was also performed in dMLH1 patients., Results: Overall, 401/3199 patients (12.5%) were MMR-deficient (dMMR); 312 (77.8%) in cohort A and 89 (22.2%) inB; 346/401 were dMLH1 (86.3%), 262/312 (83.9%) in cohort A and 84/89 (94.3%) in B. In Cohort A, 91/312 (29.1%) dMMR patients were referred to GC, 69/91 (75.8%) were in the dMLH1 group; 57/69 (82.6%) dMLH1 patients underwent GT and 1/57 (1.7%) had LS. In Cohort B, 3/84 dMLH1 patients did not undergo BRAF testing. Three BRAF wt and not hypermethylated of the remaining 81 dMLH1 patients were referred to GC and GT, and one had LS. This diagnostic pathway reduced GC referrals by 96% (78/81) in Cohort B and increased the diagnostic yield of GT by about 20 times., Conclusion: Our findings support RefT in dMLH1 CRC patients within the LS diagnostic pathway, as it reduces the number of GC sessions needed and increases the diagnostic yield of GT., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
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- 2024
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28. BRAF V600E immunohistochemistry can reliably substitute BRAF molecular testing in the Lynch syndrome screening algorithm in colorectal cancer.
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Grillo F, Paudice M, Pigozzi S, Dono M, Lastraioli S, Lugaresi M, Bozzano S, Tognoni C, Ali M, Sciallero S, Puccini A, Fassan M, and Mastracci L
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- Humans, Immunohistochemistry, Proto-Oncogene Proteins B-raf genetics, Proto-Oncogene Proteins B-raf metabolism, Early Detection of Cancer, Molecular Diagnostic Techniques, Algorithms, DNA Mismatch Repair, Mutation, Colorectal Neoplasms, Hereditary Nonpolyposis diagnosis, Colorectal Neoplasms, Hereditary Nonpolyposis genetics, Colorectal Neoplasms diagnosis, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Brain Neoplasms, Neoplastic Syndromes, Hereditary
- Abstract
Aims: The Lynch syndrome (LS) screening algorithm requires BRAF testing as a fundamental step to distinguish sporadic from LS-associated colorectal carcinomas (CRC). BRAF testing by immunohistochemistry (IHC) has shown variable results in the literature. Our aim was to analyse concordance between BRAF
V600E IHC and BRAF molecular analysis in a large, mono-institutional CRC whole-slide, case series with laboratory validation., Methods and Results: MisMatch repair (MMR) protein (hMLH1, hPMS2, hMSH2, and hMSH6) and BRAFV600E IHC were performed on all unselected cases of surgically resected CRCs (2018-2023). An in-house validation study for BRAFV600E IHC was performed in order to obtain optimal IHC stains. BRAFVV600E IHC was considered negative (score 0), positive (scores 2-3), and equivocal (score 1). Interobserver differences in BRAFV600E IHC scoring were noted in the first 150 cases prospectively collected. Nine-hundred and ninety CRCs cases (830 proficient (p)MMR/160 deficient (d)MMR) were included and all cases performed BRAFV600E IHC (BRAFV600E IHC-positive 13.5% of all series; 66.3% dMMR cases; 3.4% pMMR cases), while 333 also went to BRAF mutation analysis. Optimal agreement in IHC scoring between pathologists (P < 0.0001) was seen; concordance between BRAFV600E IHC and BRAF molecular analysis was extremely high (sensitivity 99.1%, specificity 99.5%; PPV 99.1%, and NPV 99.5%). Discordant cases were reevaluated; 1 score 3 + IHC/wildtype case was an interpretation error and one score 0 IHC/mutated case was related to heterogenous BRAFV600E IHC expression. Among the 12 IHC-equivocal score 1+ cases (which require BRAF molecular analysis), three were BRAF-mutated and nine BRAF-wildtype., Conclusion: BRAFV600E IHC can be used as a reliable surrogate of molecular testing after stringent in-house validation., (© 2023 The Authors. Histopathology published by John Wiley & Sons Ltd.)- Published
- 2024
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29. Collective action against corruption in Western and non-Western countries: cross-cultural implications of the Axiological-Identitary Collective Action Model.
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Grigoryev D, Gallyamova A, Conway LG 3rd, Zubrod A, Sabucedo JM, Dono M, Batkhina A, and Boehnke K
- Abstract
People sometimes protest government corruption, yet our current understanding of why they do so is culturally constrained. Can we separate pancultural factors influencing people's willingness to protest government corruption from factors culturally specific to each socioecological context? Surprisingly little cross-cultural data exist on this important question. To fill this gap, we performed a cross-cultural test of the Axiological-Identitary Collective Action Model (AICAM) regarding the intention to protest against corruption. As a collective action framework, AICAM integrates three classical antecedents of collective action (injustice, efficacy, identity) with axiological variables (ideology and morality). A total sample of 2,316 participants from six countries (Nigeria, Russia, India, Spain, United States, Germany) in a multilevel analysis of AICAM predictions showed that the positive relationship of the intention to protest corruption with moral obligation, system-based anger, and national identification can be considered pancultural. In contrast, the relationships between system justification and perceived efficacy are culturally specific. System justification negatively predicted the intention to participate only in countries with high levels of wealth, while perceived efficacy positively predicted it only in countries perceived as less corrupt. These findings highlight the importance of accounting features of socioecology and separating pancultural from culture-specific effects in understanding collective action., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Grigoryev, Gallyamova, Conway, Zubrod, Sabucedo, Dono, Batkhina and Boehnke.)
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- 2024
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30. 'Only My Truth Can Save Us All': The Impact of Socioeconomic Threat and its Emotional Appraisals on the Monopoly on Truth and Political Extremism.
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Dono M, Alzate M, and Sabucedo JM
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- Humans, Anger, Intention, Socioeconomic Factors, Emotions, COVID-19
- Abstract
Background: Political extremism is one of the main threats to democratic societies and it has been related to socioeconomic threats like COVID-19. We argue that socioeconomic threat perceptions increase Monopoly on Truth (MoT), a tendency to conceive personal values as undeniable truths that are worthy of being imposed. MoT will then prompt a rise in extremist intentions., Method: These hypotheses were tested in two experimental designs ( N = 274 & 484). Study 1 manipulated socioeconomic threat, while Study 2 added a manipulation of the emotional appraisal of that threat., Results: In Study 1, MoT scores were significantly different and higher in the high-threat condition. In Study 2, threat levels did not cause statistically significant differences when emotional appraisal of the threat was manipulated, with anger causing MoT to increase. Study 2 also showed that MoT works as a precursor of extremist intentions., Conclusions: Socioeconomic threats and their emotional appraisals raise MoT and, indirectly, extremism. Beyond deepening our understanding of the causes of MoT, the current findings highlight the implications of different narratives framing socioeconomic crises that may become a facilitating factor of extremism.
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- 2024
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31. Front-line liquid biopsy for early molecular assessment and treatment of hospitalized lung cancer patients.
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Parisi F, De Luca G, Mosconi M, Lastraioli S, Dellepiane C, Rossi G, Puglisi S, Bennicelli E, Barletta G, Zullo L, Santamaria S, Mora M, Ballestrero A, Montecucco F, Bellodi A, Del Mastro L, Lambertini M, Barisione E, Cittadini G, Tagliabue E, Spagnolo F, Tagliamento M, Coco S, Dono M, and Genova C
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- Humans, Liquid Biopsy methods, Male, Female, Middle Aged, Aged, Mutation, Hospitalization, Biomarkers, Tumor genetics, Aged, 80 and over, Adult, Lung Neoplasms genetics, Lung Neoplasms pathology, Lung Neoplasms diagnosis, Lung Neoplasms therapy, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung pathology, Carcinoma, Non-Small-Cell Lung diagnosis, Carcinoma, Non-Small-Cell Lung therapy, High-Throughput Nucleotide Sequencing methods
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Background: Molecular characterization is pivotal for managing non-small cell lung cancer (NSCLC), although this process is often time-consuming and patients' conditions might worsen while molecular analyses are processed. Our primary aim was to evaluate the performance of "up-front" next-generation sequencing (NGS) through liquid biopsy (LB) of hospitalized patients with newly detected lung neoplasm in parallel with conventional diagnosis. The secondary aim included longitudinal monitoring through LB of patients with oncogenic alterations at baseline., Methods: We enrolled 47 consecutive patients immediately after hospitalization and radiological detection of symptomatic lung neoplasm. LB from peripheral blood was performed at baseline, in parallel with conventional biopsy (CB), when feasible. Additionally, LBs were repeated during treatment in patients with actionable gene alterations at baseline. Oncomine™ Lung cfTNA Research Assay panel was employed for processing plasma samples in NGS., Results: 47 hospitalized patients were enrolled. LB identified 28 patients with gene alterations, including mutations of EGFR (n = 7), KRAS (n = 12), ERBB2 (n = 1), TP53 (n = 2), BRAF (n = 1), one ALK rearrangement, and 4 patients with combined mutations involving EGFR, KRAS and PIK3CA. LB and CB were consistent, except for two patients. Three patients with positive LB for oncogenic drivers did not undergo CB due to contraindications. Median time to molecular results after LB was significantly lower compared to time to molecular report after CB (11 versus 22 days, p < 0.001)., Conclusions: Despite limited numbers, our study supports the role of front-line LB for improving management of symptomatic patients with lung cancer, potentially leading to early targeted therapy initiation., Competing Interests: Declaration of competing interest Chiara Dellepiane has received honoraria for consulting activities of Astra Zeneca, Merck Sharp and Dohme, Bristol-Myers-Squibb, Roche. Francesco Spagnolo has been an advisory boards member for MSD, Novartis, Pierre Fabre, Sun Pharma, Philogen and has received lecture fees/honoraria for consulting activities of BMS, MSD, Novartis, Pierre Fabre, Merck, Sanofi, Sun Pharma, IGEA. Matteo Lambertini reports advisory role for Roche, Lilly, Novartis, Astrazeneca, Pfizer, Seagen, Gilead, MSD and Exact Sciences and speaker honoraria from Roche, Lilly, Novartis, Pfizer, Sandoz, Libbs, Daiichi Sankyo, Knight and Takeda, Travel Grants from Gilead and Daiichi Sankyo, and research support (to the Institution) from Gilead outside the submitted work. Lucia Del Mastro has received honoraria for consulting activities, speaker bureau or advisory boards from Roche, Novartis, Pfizer, Eli Lilly, Astra Zeneca, Merck-Sharp-Dohme, Seagen, Gilead, Pierre Fabre, Eisai, Exact sciences, Ipsen, GSK, Agendia, Stemline Menarini, Daiichi Sakyo. Carlo Genova has received honoraria for consulting activities, speaker bureau or advisory boards from Astra Zeneca, Bristol-Myers-Squibb, Eli Lilly, Janssen, Merck Sharp and Dohme, Novartis, Roche, Sanofi, Takeda. The other authors declare no conflicts of interest., (Copyright © 2024. Published by Elsevier Ltd.)
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- 2024
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32. Stem-like signatures in human meningioma cells are under the control of CXCL11/CXCL12 chemokine activity.
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Barbieri F, Bajetto A, Dellacasagrande I, Solari A, Würth R, Fernandez V, Rancati S, Ceresa D, Appolloni I, De Luca G, Dono M, Nozza P, Schiapparelli P, Gambaro M, Fiaschi P, Gaggero G, Costanzo N, Thellung S, Malatesta P, Pagano A, Zona G, De Pietri Tonelli D, and Florio T
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- Humans, Chemokine CXCL12 genetics, Receptors, CXCR4 genetics, Signal Transduction, Chemokine CXCL11, Meningioma, Receptors, CXCR genetics, Brain Neoplasms, Meningeal Neoplasms
- Abstract
Background: Meningiomas are mainly benign brain tumors, although about 20% of histologically benign cases are clinically aggressive and recur after resection. We hypothesize that meningioma brain invasiveness and recurrence may be related to the presence of cancer stem cells and their high responsiveness to the CXCL12-CXCR4/CXCR7 chemokine axis. The aim of this study was to isolate meningioma stem cells from human samples, characterize them for biological features related to malignant behavior, and to identify the role of CXCR4/CXCR7 in these processes., Methods: Meningioma stem cells were isolated from patient-derived primary cultures in stem cell-permissive conditions, and characterized for phenotype, self-renewal, proliferation and migration rates, vasculogenic mimicry (VM), and in vivo tumorigenesis, in comparison with differentiated meningioma cells and stem-like cells isolated from normal meninges. These cell populations were challenged with CXCL12 and CXCL11 and receptor antagonists to define the chemokine role in stem cell-related functions., Results: Stem-like cells isolated from meningioma cultures display higher proliferation and migration rates, and VM, as compared to meningioma non-stem cells or cells isolated from normal meninges and were the only tumorigenic population in vivo. In meningioma cells, these stem-like functions were under the control of the CXCR4/CXCR7 chemokine axis., Conclusions: We report a role for CXCL11 and CXCL12 in the control of malignant features in stem-like cells isolated from human meningioma, providing a possible basis for the aggressive clinical behavior observed in subsets of these tumors. CXCR4/CXCR7 antagonists might represent a useful approach for meningioma at high risk of recurrence and malignant progression., (© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2023
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33. The spectrum of subclonal TP53 mutations in chronic lymphocytic leukemia: A next generation sequencing retrospective study.
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De Luca G, Cerruti G, Lastraioli S, Conte R, Ibatici A, Di Felice N, Morabito F, Monti P, Fronza G, Matis S, Colombo M, Fabris S, Ciarrocchi A, Neri A, Menichini P, Ferrarini M, Nozza P, Fais F, Cutrona G, and Dono M
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- Humans, Tumor Suppressor Protein p53 genetics, Retrospective Studies, High-Throughput Nucleotide Sequencing, Leukemia, Lymphocytic, Chronic, B-Cell genetics
- Abstract
Chronic lymphocytic leukemia (CLL) is a hematological disorder with complex clinical and biological behavior. TP53 mutational status and cytogenetic assessment of the deletion of the corresponding locus (17p13.1) are considered the most relevant biomarkers associated with pharmaco-predictive response, chemo-refractoriness, and worse prognosis in CLL patients. The implementation of Next Generation Sequencing (NGS) methodologies in the clinical laboratory allows for comprehensively analyzing the TP53 gene and detecting mutations with allele frequencies ≤10%, that is, "subclonal mutations". We retrospectively studied TP53 gene mutational status by NGS in 220 samples from 171 CLL patients. TP53 mutations were found in 60/220 (27.3%) samples and 47/171 (27.5%) patients. Interestingly, subclonal mutations could be detected in 31/60 samples (51.7%) corresponding to 25 patients (25/47, 53.2%). We identified 44 distinct subclonal TP53 mutations clustered in the central DNA-binding domain of p53 protein (exons 5-8, codons 133-286). Missense mutations were predominant (>80%), whereas indels, nonsense, and splice site variants were less represented. All subclonal TP53 variants but one [p.(Pro191fs)] were already described in NCI and/or Seshat databases as "damaging" and/or "probably damaging" mutations (38/44, 86% and 6/44, 14%, respectively). Longitudinal samples were available for 37 patients. Almost half of them displayed at least one TP53 mutant subclone, which could be alone (4/16, 25%) or concomitant with other TP53 mutant clonal ones (12/16, 75%); different patterns of mutational dynamics overtimes were documented. In conclusion, utilization of NGS in our "real-life" cohort of CLL patients demonstrated an elevated frequency of subclonal TP53 mutations. This finding indicates the need for precisely identifying these mutations during disease since the clones carrying them may become predominant and be responsible for therapy failures., (© 2022 The Authors. Hematological Oncology published by John Wiley & Sons Ltd.)
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- 2022
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34. A Circulating Risk Score, Based on Combined Expression of Exo-miR-130a-3p and Fibrinopeptide A, as Predictive Biomarker of Relapse in Resectable Non-Small Cell Lung Cancer Patients.
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Marconi S, Croce M, Chiorino G, Rossi G, Guana F, Profumo A, Ostano P, Alama A, Longo L, De Luca G, Dono M, Dal Bello MG, Ponassi M, Rosano C, Romano P, Cavalieri Z, Grassi M, Tagliamento M, Zullo L, Venturi C, Dellepiane C, Mastracci L, Bennicelli E, Pronzato P, Genova C, and Coco S
- Abstract
To date, the 5-year overall survival rate of 60% for early-stage non-small cell lung cancer (NSCLC) is still unsatisfactory. Therefore, reliable prognostic factors are needed. Growing evidence shows that cancer progression may depend on an interconnection between cancer cells and the surrounding tumor microenvironment; hence, circulating molecules may represent promising markers of cancer recurrence. In order to identify a prognostic score, we performed in-depth high-throughput analyses of plasma circulating markers, including exosomal microRNAs (Exo-miR) and peptides, in 67 radically resected NSCLCs. The miRnome profile selected the Exo-miR-130a-3p as the most overexpressed in relapsed patients. Peptidome analysis identified four progressively more degraded forms of fibrinopeptide A (FpA), which were depleted in progressing patients. Notably, stepwise Cox regression analysis selected Exo-miR-130a-3p and the greatest FpA (2-16) to build a score predictive of recurrence, where high-risk patients had 18 months of median disease-free survival. Moreover, in vitro transfections showed that higher levels of miR-130a-3p lead to a deregulation of pathways involved in metastasis and angiogenesis, including the coagulation process and metalloprotease increase which might be linked to FpA reduction. In conclusion, by integrating circulating markers, the identified risk score may help clinicians predict early-stage NSCLC patients who are more likely to relapse after primary surgery.
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- 2022
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35. Targeting PIK3CA Actionable Mutations in the Circulome: A Proof of Concept in Metastatic Breast Cancer.
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Cardinali B, De Luca G, Tasso R, Coco S, Garuti A, Buzzatti G, Sciutto A, Arecco L, Villa F, Carli F, Reverberi D, Quarto R, Dono M, and Del Mastro L
- Subjects
- Biomarkers, Tumor genetics, Class I Phosphatidylinositol 3-Kinases genetics, Epithelial Cell Adhesion Molecule genetics, Female, Humans, Mutation, Pilot Projects, Breast Neoplasms genetics, Breast Neoplasms pathology, Circulating Tumor DNA genetics, Neoplastic Cells, Circulating pathology
- Abstract
The study of circulating cancer-derived components (circulome) is considered the new frontier of liquid biopsy. Despite the recognized role of circulome biomarkers, their comparative molecular profiling is not yet routine. In advanced breast cancer (BC), approximately 40% of hormone-receptor-positive, HER2-negative BC cases harbor druggable PIK3CA mutations suitable for combined alpelisib/fulvestrant treatment. This pilot study investigates PIK3CA mutations in circulating tumor DNA (ctDNA), tumor cells (CTCs), and extracellular vesicles (EVs) with the aim of determining which information on molecular targetable profiling could be recollected in each of them. The in-depth molecular analysis of four BC patients demonstrated, as a proof-of-concept study, that it is possible to retrieve mutational information in the three components. Patient-specific PIK3CA mutations were found in both tissue and ctDNA and in 3/4 cases, as well as in CTCs, in the classical population (large-sized CD45-/EpCAM+/- cells), and/or in the "non-conventional" sub-population (smaller-sized CD44+/EpCAM-/CD45- cells). Consistent mutational profiles of EVs with CTCs suggest that they may have been released by CTCs. This preliminary evidence on the molecular content of the different circulating biomaterials suggests their possible function as a mirror of the intrinsic heterogeneity of BC. Moreover, this study demonstrates, through mutational assessment, the tumor origin of the different CTC sub-populations sustaining the translational value of the circulome for a more comprehensive picture of the disease.
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- 2022
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36. Potential application of cryobiopsy for histo-molecular characterization of mediastinal lymph nodes in patients with thoracic malignancies: a case presentation series and implications for future developments.
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Genova C, Tagliabue E, Mora M, Aloè T, Dono M, Salvi S, Zullo L, and Barisione E
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- Aged, Bronchoscopy methods, Carcinoma, Non-Small-Cell Lung pathology, Humans, Lung Neoplasms pathology, Lymphoma, Follicular pathology, Male, Middle Aged, Neoplasm Staging, Endoscopic Ultrasound-Guided Fine Needle Aspiration methods, Lymph Nodes pathology, Mediastinum pathology, Thoracic Neoplasms pathology
- Abstract
Background: The management of non-small cell lung cancer (NSCLC) has become increasingly complex due to the evolution of personalized medicine approaches. Such approaches are characterized by the necessity of adequate tumor samples; hence, improved biopsy techniques are needed. Transbronchial lung cryobiopsy is a novel endoscopic procedure designed to collect peripheral pulmonary tissue, and it is currently employed in interstitial lung diseases. The use of this technique in oncology might result in improved mediastinum staging and molecular characterizations; however, available data involving the use of a cryoprobe on mediastinal lymph nodes are still limited., Case Presentation: Here we present a series of five consecutive patients who underwent endoscopic assessment of mediastinal lymph nodes for oncologic reasons. All patients were subjected both to endobronchial ultrasound-guided transbronchial needle aspiration (EBUS TBNA) and cryobiopsy of mediastinal lymph nodes during the same procedure, and no complications were observed. In three of the reported cases, both cryobiopsy and cell block from EBUS TBNA were positive, while in one case cryobiopsy was not diagnostic and EBUS TBNA was negative; moreover, one case showed discordance between the procedures, as cryobiopsy was negative and cell block obtained from multiple stations was diagnostic for small cell lung cancer. In one case involving a patient treated for lymphoma, cryobiopsy provided more complete histologic characterization, and in another case involving a patient affected by NSCLC cryobiopsy provided more material for molecular analyses., Conclusion: This case presentation series suggests that cryobiopsy, which has been generally used on peripheral lung lesions so far, is a feasible and safe approach for diagnosis and staging of mediastinal lymph nodal involvement, especially when station 7 is involved. Compared to EBUS TBNA, cryobiopsy might provide more adequate histological samples, with a possible impact on molecular characterizations and, therefore, therapeutic decisions. However, the learning curve of the procedure has not to be understated and optimal protocols for implementing this technique are needed. In our opinion, further studies designed to integrate the routine use of cryobiopsy in current practice for solid and eventually hematologic tumors with mediastinal lymph node involvement are warranted., (© 2022. The Author(s).)
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- 2022
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37. The storm of NGS in NSCLC diagnostic-therapeutic pathway: How to sun the real clinical practice.
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De Maglio G, Pasello G, Dono M, Fiorentino M, Follador A, Sciortino M, Malapelle U, and Tiseo M
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- High-Throughput Nucleotide Sequencing, Humans, Mutation, Precision Medicine, Carcinoma, Non-Small-Cell Lung diagnosis, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung therapy, Lung Neoplasms diagnosis, Lung Neoplasms genetics, Lung Neoplasms therapy
- Abstract
The increasing number of approved drugs along with next generation sequencing (NGS) technologies look out as potential revolution of biomolecular characterization of non-small-cell lung cancer (NSCLC). Nevertheless, several aspects impact on success rate of NGS in clinical practice: a multidisciplinary approach and thorough knowledge of strengths and limits of each technologic diagnostic tool are required. Crucial preliminary step is the selection of the best available sample before testing, aware of clinical condition and setting of disease. Genomic data should be than integrated in the clinical context and matched with available therapeutic options; Molecular Tumor Boards (MTB) are worldwide emerging interdisciplinary groups implemented to transfer the impact of precision medicine in clinical practice. In order to guarantee equity in treatment, these considerations should find their application widely and rapidly. Aim of this review is offering an overview of emerging biomarkers, relative upcoming targeted drugs, and new diagnostic chances with an authors' perspective about a real-life diagnostic-therapeutic algorithm useful for daily clinical practice., (Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)
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- 2022
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38. The Opportunities and Challenges of Molecular Tagging Next-Generation Sequencing in Liquid Biopsy.
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De Luca G and Dono M
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- High-Throughput Nucleotide Sequencing, Humans, Laboratories, Clinical, Liquid Biopsy, Cell-Free Nucleic Acids, Skin Neoplasms
- Abstract
Liquid biopsy (LB) is a promising tool that is rapidly evolving as a standard of care in early and advanced stages of cancer settings. Next-generation sequencing (NGS) methods have become essential in molecular diagnostics and clinical laboratories dealing with LB analytes, i.e., cell-free DNA and RNA. The sensitivity and high-throughput capacity of NGS enable us to overcome technical issues that are mainly attributable to low-abundance (below 1% mutated allelic frequency) tumour genetic material circulating within biological fluids. In this context, the introduction of unique molecular identifiers (UMIs), also known as molecular barcodes, applied to various NGS platforms greatly improved the characterization of rare genetic alterations, as they resulted in a drastic reduction in background noise while maintaining high levels of positive predictive value and sensitivity. Different UMI strategies have been developed, such as single (e.g., safe-sequencing system, Safe-SeqS) or double (duplex-sequencing system, Duplex-Seq) strand-based labelling, and, currently, considerable results corroborate their potential implementation in a routine laboratory. Recently, the US Food and Drug Administration approved the clinical use of two comprehensive UMI-based NGS assays (FoundationOne Liquid CDx and Guardant360 CDx) in cfDNA mutational assessment. However, to definitively translate LB into clinical practice, UMI-based NGS protocols should meet certain feasibility requirements in terms of cost-effectiveness, wet laboratory performance and easy access to web-source and bioinformatic tools for downstream molecular data., (© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
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- 2021
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39. Predicting Collective Action in a Secessionist Context: Different Motives for Two Opposed Stances.
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Dono M, Alzate M, and Sabucedo JM
- Abstract
Engagement in collective action is essential in the scenario of a secessionist struggle. In this scenario, two groups contend for an incompatible goal and one of them is favoured by the current status quo. Therefore, this context represents an excellent opportunity to compare the motives for participation among two groups whose situation and objectives differ drastically. We examined the motivations to participate in collective action of Catalan participants in the days leading to the independence referendum held in Catalonia (Spain) on the first of October 2017 ( n = 719). As hypothesized, participation predicted by different motivations for each group. Regarding participation in the referendum, Catalan identity was the only predictor among pro-independence ranks, while those against independence showed a solidarity-based motivation. This work contributes to the literature by adapting previously researched collective action motivations to the context of a secessionist contention and providing evidence of their effect. Crucially, the motivations are different between supporters and opponents of independence, highlighting the need for examining the status and the stance on the system of groups when studying collective action., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Dono, Alzate and Sabucedo.)
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- 2021
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40. Radiomic Detection of EGFR Mutations in NSCLC.
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Rossi G, Barabino E, Fedeli A, Ficarra G, Coco S, Russo A, Adamo V, Buemi F, Zullo L, Dono M, De Luca G, Longo L, Dal Bello MG, Tagliamento M, Alama A, Cittadini G, Pronzato P, and Genova C
- Subjects
- Adenocarcinoma diagnostic imaging, Adenocarcinoma drug therapy, Adenocarcinoma genetics, Adenocarcinoma pathology, Area Under Curve, Carcinoma, Non-Small-Cell Lung diagnostic imaging, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung pathology, ErbB Receptors antagonists & inhibitors, Female, Humans, Lung Neoplasms diagnostic imaging, Lung Neoplasms drug therapy, Lung Neoplasms pathology, Male, ROC Curve, Reproducibility of Results, Tomography, X-Ray Computed methods, Algorithms, Carcinoma, Non-Small-Cell Lung genetics, ErbB Receptors genetics, Lung Neoplasms genetics, Machine Learning, Mutation
- Abstract
Radiomics is defined as the use of automated or semi-automated post-processing and analysis of multiple features derived from imaging exams. Extracted features might generate models able to predict the molecular profile of solid tumors. The aim of this study was to develop a predictive algorithm to define the mutational status of EGFR in treatment-naïve patients with advanced non-small cell lung cancer (NSCLC). CT scans from 109 treatment-naïve patients with NSCLC (21 EGFR -mutant and 88 EGFR -wild type) underwent radiomics analysis to develop a machine learning model able to recognize EGFR -mutant from EGFR -WT patients via CT scans. A "test-retest" approach was used to identify stable radiomics features. The accuracy of the model was tested on an external validation set from another institution and on a dataset from the Cancer Imaging Archive (TCIA). The machine learning model that considered both radiomic and clinical features (gender and smoking status) reached a diagnostic accuracy of 88.1% in our dataset with an AUC at the ROC curve of 0.85, whereas the accuracy values in the datasets from TCIA and the external institution were 76.6% and 83.3%, respectively. Furthermore, 17 distinct radiomics features detected at baseline CT scan were associated with subsequent development of T790M during treatment with an EGFR inhibitor. In conclusion, our machine learning model was able to identify EGFR -mutant patients in multiple validation sets with globally good accuracy, especially after data optimization. More comprehensive training sets might result in further improvement of radiomics-based algorithms. SIGNIFICANCE: These findings demonstrate that data normalization and "test-retest" methods might improve the performance of machine learning models on radiomics images and increase their reliability when used on external validation datasets., (©2020 American Association for Cancer Research.)
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- 2021
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41. Time to first treatment and P53 dysfunction in chronic lymphocytic leukaemia: results of the O-CLL1 study in early stage patients.
- Author
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Monti P, Lionetti M, De Luca G, Menichini P, Recchia AG, Matis S, Colombo M, Fabris S, Speciale A, Barbieri M, Gentile M, Zupo S, Dono M, Ibatici A, Neri A, Ferrarini M, Fais F, Fronza G, Cutrona G, and Morabito F
- Subjects
- Female, Humans, Leukemia, Lymphocytic, Chronic, B-Cell therapy, Male, Middle Aged, Mutation, Prospective Studies, Genes, p53, Leukemia, Lymphocytic, Chronic, B-Cell genetics, Time-to-Treatment
- Abstract
Chronic lymphocytic leukaemia (CLL) is characterised by a heterogeneous clinical course. Such heterogeneity is associated with a number of markers, including TP53 gene inactivation. While TP53 gene alterations determine resistance to chemotherapy, it is not clear whether they can influence early disease progression. To clarify this issue, TP53 mutations and deletions of the corresponding locus [del(17p)] were evaluated in 469 cases from the O-CLL1 observational study that recruited a cohort of clinically and molecularly characterised Binet stage A patients. Twenty-four cases harboured somatic TP53 mutations [accompanied by del(17p) in 9 cases], 2 patients had del(17p) only, and 5 patients had TP53 germ-line variants. While del(17p) with or without TP53 mutations was capable of significantly predicting the time to first treatment, a reliable measure of disease progression, TP53 mutations were not. This was true for cases with high or low variant allele frequency. The lack of predictive ability was independent of the functional features of the mutant P53 protein in terms of transactivation and dominant negative potential. TP53 mutations alone were more frequent in patients with mutated IGHV genes, whereas del(17p) was associated with the presence of adverse prognostic factors, including CD38 positivity, unmutated-IGHV gene status, and NOTCH1 mutations.
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- 2020
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42. TP53 dysfunction in chronic lymphocytic leukemia: clinical relevance in the era of B-cell receptors and BCL-2 inhibitors.
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Morabito F, Gentile M, Monti P, Recchia AG, Menichini P, Skafi M, Atrash M, De Luca G, Bossio S, Al-Janazreh H, Galimberti S, Salah Z, Morabito L, Mujahed A, Hindiyeh M, Dono M, Fais F, Cutrona G, Neri A, Tripepi G, Fronza G, and Ferrarini M
- Subjects
- High-Throughput Nucleotide Sequencing, Humans, Leukemia, Lymphocytic, Chronic, B-Cell genetics, Leukemia, Lymphocytic, Chronic, B-Cell pathology, Mutation, Proto-Oncogene Proteins c-bcl-2 antagonists & inhibitors, Antineoplastic Agents pharmacology, Leukemia, Lymphocytic, Chronic, B-Cell drug therapy, Tumor Suppressor Protein p53 genetics
- Abstract
Introduction: Patients with TP53 dysfunction, assessed by del(17p) or TP53 mutations, respond poorly to chemo-immunotherapy and fare better with the new therapies (BCR and BCL-2 inhibitors); however, it is unclear whether their response is similar to that of patients without anomalies or whether there is currently an adequate determination of TP53 dysfunction., Area Covered: A literature search was undertaken on clinical trials and real-world experience data on patients with TP53 dysfunction treated with different protocols. Moreover, data on the TP53 biological function and on the tests currently employed for its assessment were reviewed., Expert Opinion: Although TP53 dysfunction has less negative influence on the new biological therapies, patients with these alterations, particularly those with biallelic inactivation of TP53 , have a worst outcome with these therapies than those without alterations. At present, a determination of TP53 , particularly with next generation sequencing (NGS) methodologies, may be sufficient for the identifications of the patients unsuitable for chemo-immunotherapy, although integration with del(17p) would be advisable. For the future, more extensive determinations of the TP53 status, including functional assays, may become part of the current armamentarium for a better patient stratification and treatment with newer protocols.
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- 2020
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43. Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling.
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De Luca G, Cardinali B, Del Mastro L, Lastraioli S, Carli F, Ferrarini M, Calin GA, Garuti A, Mazzitelli C, Zupo S, and Dono M
- Subjects
- Cell Line, Tumor, Class I Phosphatidylinositol 3-Kinases genetics, Feasibility Studies, Female, High-Throughput Nucleotide Sequencing, Humans, MCF-7 Cells, Precision Medicine, Proto-Oncogene Proteins p21(ras) genetics, Single-Cell Analysis, Tumor Suppressor Protein p53 genetics, Biomarkers, Tumor genetics, Breast Neoplasms genetics, DNA Mutational Analysis methods, Neoplastic Cells, Circulating chemistry
- Abstract
Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect CTCs mutations while skipping the WGA step. MDA-MB-231 and MCF-7 cell lines, as well as leukocytes, were sorted into pools (2-5 cells) using a DEPArray™ system and were employed to set up the overall NGS procedure. A substantial reduction of reagent volume for the preparation of libraries was performed, in order to fit the limited DNA templates directly derived from cell lysates. Known variants in TP53 , KRAS , and PIK3CA genes were detected in almost all the cell line pools (35/37 pools, 94.6%). No additional alterations, other than those which were expected, were found in all tested pools and no mutations were detected in leukocytes. The translational value of the optimized NGS workflow is confirmed by sequencing CTCs pools isolated from eight breast cancer patients and through the successful detection of variants. In conclusion, this study shows that the proposed NGS molecular tagging approach is technically feasible and, compared to traditional NGS approaches, has the advantage of filtering out the artifacts generated during library amplification, allowing for the reliable detection of mutations and, thus, making it highly promising for clinical use.
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- 2020
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44. An H- TERT Mutated Skin Metastasis as First Occurrence in a Case of Follicular Thyroid Carcinoma.
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Monti E, Dono M, Gonella E, Spina B, Pitto F, Petrogalli F, Conte L, Ambrosetti E, Minuto MN, Ansaldo GL, Morbelli S, Zupo S, and Giusti M
- Abstract
Differentiated thyroid cancer arising from thyroid follicular epithelial cells is the most frequent endocrine malignancy, and skin metastases are very rare. We describe a case of a 70-year-old women with a history of an indeterminate thyroid nodule on cytology. A painless, erythematous skin nodule of about 7 mm diameter was removed from the scalp and diagnosed as a metastasis from thyroid cancer. After total thyroidectomy, a histological diagnosis of follicular thyroid cancer was made. Two cycles of radioactive iodine were performed. Both the follicular thyroid carcinoma (FTC) and the metastasis were investigated for the presence of BRAF/RAS and TERT promoter mutations. The results showed that the cutaneous metastasis was BRAF wild-type and TERT promoter-mutated (position g.1,295,228 C>T); in contrast, the primary thyroid lesion was negative for both molecular markers.
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- 2019
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45. Axiological-Identitary Collective Action Model (AICAM): A new integrative perspective in the analysis of protest.
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Sabucedo JM, Dono M, Grigoryev D, Gómez-Román C, and Alzate M
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- Civil Disorders statistics & numerical data, Female, Humans, Male, Social Identification, Surveys and Questionnaires, Civil Disorders psychology, Mass Behavior, Models, Psychological, Morals, Motivation
- Abstract
Current predictive models of collective action have devoted little attention to personal values, such as morals or ideology. The present research addresses this issue by incorporating a new axiological path in a novel predictive model of collective action, named AICAM. The axiological path is formed by two constructs: ideology and moral obligation. The model has been tested for real normative participation (Study 1) and intentional non-normative participation (Study 2). The sample for Study 1 included 531 randomly selected demonstrators and non-demonstrators at the time of a protest that took place in Madrid, May 2017. Study 2 comprised 607 randomly selected participants who filled out an online questionnaire. Structural equation modelling analysis was performed in order to examine the fit and predictive power of the model. Results show that the model is a good fit in both studies. It has also been observed that the new model entails a significant addition of overall effect size when compared with alternative models, including SIMCA. The present research contributes to the literature of collective action by unearthing a new, independent path towards collective action that is nonetheless compatible with previous motives. Implications for future research are discussed, mainly stressing the need to include moral and ideological motives in the study of collective action engagement., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
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46. Tag-based next generation sequencing: a feasible and reliable assay for EGFR T790M mutation detection in circulating tumor DNA of non small cell lung cancer patients.
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Dono M, De Luca G, Lastraioli S, Anselmi G, Dal Bello MG, Coco S, Vanni I, Grossi F, Vigani A, Genova C, Ferrarini M, Ravetti JL, and Zupo S
- Subjects
- Female, Humans, Male, Mutation genetics, Carcinoma, Non-Small-Cell Lung genetics, Circulating Tumor DNA genetics, ErbB Receptors genetics, High-Throughput Nucleotide Sequencing methods, Lung Neoplasms genetics
- Abstract
Background: The demonstration of EGFR T790M gene mutation in plasma is crucial to assess the eligibility of Non Small Cell Lung Cancer (NSCLC) patients, who have acquired resistance to first or second generation Tyrosine Kinase Inhibitors (TKIs), to receive a subsequent treatment with osimertinib. Since circulating tumor DNA (ctDNA) is present in very low amounts in plasma, high sensitive and specific methods are required for molecular analysis. Improving sensitivity of T790M mutation detection in plasma ctDNA enables a larger number of NSCLC patients to receive the appropriate therapy without any further invasive procedure., Methods: A tag-based next generation sequencing (NGS) platform capable of tagging rare circulating tumor DNA alleles was employed in this study for the identification of T790M mutation in 42 post-TKI NSCLC patients., Results: Compared to Real Time PCR, tag-based NGS improved the T790M detection rate (42.85% versus 21.4%, respectively), especially in those cases with a low median mutation abundance (i.e. 0.24, range 0.07-0.78). Moreover, the tag-based NGS identified EGFR activating mutations more efficiently than Real Time PCR (85.7% versus 61.9% detection rate, respectively), particularly of the L858R variant type (0.06-0.75 mutation abundance range). Patients in whom the T790M mutation was detected in plasma, achieved an objective response to osimertinib (9/14, 64.28%)., Conclusions: Tag-based NGS represents an accurate and sensitive tool in a clinical setting for non-invasive assessment and monitoring of T790M variant in NSCLC patients.
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- 2019
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47. Development and validation of the Monopoly on Truth Scale. A measure of political extremism.
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Dono M, Alzate M, Seoane G, and Sabucedo JM
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- Female, Humans, Male, Pilot Projects, Young Adult, Attitude, Morals, Political Activism trends, Self Report
- Abstract
Background: Extreme political attitudes have been on the rise since the economic and political crisis of 2008. This surge of extremism constitutes a real threat, as attitudes like these are dangerous for the peaceful, democratic functioning of society. A new cognitive style, Monopoly on Truth has been proposed, based mainly on the concept of naïve realism., Method: The development and validation of a scale for this new construct is the main objective of this study. A pilot study (N=209) was performed in order to gather the items that make up the final scale; and a main study (N=369) was conducted to test the validity and predictive power of the scale., Results: The validation is successful as the scale shows good reliability scores, while also proving to be linked to extremism-related constructs. Additionally, the scale shows signs of not being ideologically biased., Conclusion: Results show the scale to be a very useful tool for studying extremism and other political trends. Future directions and other implications of the Monopoly on Truth are also discussed.
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- 2018
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48. The Importance of Protesters' Morals: Moral Obligation as a Key Variable to Understand Collective Action.
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Sabucedo JM, Dono M, Alzate M, and Seoane G
- Abstract
Collective action and protest have become a normalized political behavior that in many cases defines the political agenda. The reasons why people take to the streets constitute a central subject within the study of social psychology. In the literature, three precedents of protest that have been established as central to the study of this phenomenon are: injustice, efficacy, and identity. But political action is also deeply related to moral values. This explains why in recent years some moral constructs have also been pointed out as predictors of collective action. Moral variables have been introduced into the literature with little consideration to how they relate to each other. Thus, work in this direction is needed. The general aim of this research is to differentiate moral obligation from moral norms and moral conviction, as well as to compare their ability to predict collective action. In order to do so, the research objectives are: (a) conceptualize and operationalize moral obligation (Study 1, N = 171); (b) test its predictive power for intention to participate in protests (Study 2, N = 622); and (c) test moral obligation in a real context (Study 3, N = 407). Results are encouraging, showing not only that moral obligation is different to moral conviction and moral norm, but also that it is a more effective predictor working both for intention and real participation. This work therefore presents moral obligation as a key precedent of protest participation, prompting its future use as a variable that can enhance existing predictive models of collective action. Results regarding other variables are also discussed.
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- 2018
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49. Cytokines can counteract the inhibitory effect of MEK-i on NK-cell function.
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Manzini C, Venè R, Cossu I, Gualco M, Zupo S, Dono M, Spagnolo F, Queirolo P, Moretta L, Mingari MC, and Pietra G
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- Acrylonitrile pharmacology, Antineoplastic Agents pharmacology, Apoptosis, Benzamides pharmacology, Cell Line, Tumor, Cell Proliferation, Cell Survival drug effects, Diphenylamine analogs & derivatives, Diphenylamine pharmacology, Down-Regulation drug effects, Flow Cytometry, Humans, Immunotherapy, Indoles pharmacology, Killer Cells, Natural metabolism, Melanoma drug therapy, Melanoma metabolism, Neoplasm Recurrence, Local drug therapy, Oncogenes, Proto-Oncogene Proteins B-raf metabolism, Signal Transduction drug effects, Skin Neoplasms metabolism, Sulfonamides pharmacology, Vemurafenib, Acrylonitrile analogs & derivatives, Aniline Compounds pharmacology, Interleukin-15 metabolism, Interleukin-2 metabolism, Killer Cells, Natural drug effects
- Abstract
Oncogene-targeted therapies based on mutated BRAF- and/or MEK-specific inhibitors have been developed for melanoma treatment. Although these drugs induce tumor regression in a high percentage of patients, clinical responses are frequently limited in time and tumors often recur. Recent studies suggested that the combination of BRAF/MEK inhibition with immunotherapy could represent a promising strategy for the cure of melanoma. NK cells are suitable effectors for tumor immunotherapy. Here we show that PLX4032 (a mutant BRAFV600 inhibitor) had no effect on the functional properties of NK cells cultured in the presence of IL-2 or IL-15. In contrast, PD0325901 (a MEK inhibitor) induced the down-regulation of the main activating NK receptors and inhibited NK cell function. Importantly, PD0325901 did not affect the anti-tumor activity of NK cells that had been exposed to a combination of IL-15 and IL-18. In addition, both PLX4032 and PD0325901 did not exert any inhibitory effect on in vitro IL-2 or IL-15 pre-activated NK cells.Our data may provide a rationale for future clinical protocols that combine IL-15/IL-18 cytokine administration with MEK inhibitors. In addition, they suggest that oncogene-targeting drugs are compatible with NK-based adoptive therapy., Competing Interests: CONFLICTS OF INTERESTS The authors declare that they have no conflicts of interest.
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- 2016
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50. BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer.
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Monti E, Bovero M, Mortara L, Pera G, Zupo S, Gugiatti E, Dono M, Massa B, Ansaldo GL, and Massimo G
- Abstract
Background. Molecular diagnostics has offered new techniques for searching for mutations in thyroid indeterminate lesions. The study's aim was to evaluate the BRAF mutations' incidence in an Italian regional population. Subjects and Methods. 70 Caucasian patients born in Liguria with indeterminate or suspicious cytological diagnoses. Results. A BRAF gene mutation was successfully analyzed in 56/70 patients. The mutation was BRAF V600E in 12/56 cases (21%) and BRAF K601E in 2/56 (4%). Of the BRAF mutated samples on cytological diagnosis (14/56 cases), 2/14 cases (14%) were benign on final histology and 12/14 (86%) were malignant. All BRAF-mutated cases on cytology that were found to be benign on histological examination carried the K601E mutation. Of the nonmutated BRAF cases (42/56, 75%) which were later found to be malignant on definitive histology, 5 cases were follicular carcinomas (36%), 3 cases were incidentally found to be papillary microcarcinomas (22%), 2 were cases papillary carcinomas (14%), 1 was case follicular variant of papillary carcinoma (7%), 1 was case medullary carcinoma (7%), 1 case was Hurtle cell tumor (7%), and 1 case was combined cell carcinoma and papillary oncocytic carcinoma (7%). Conclusions. The presence of the BRAF V600E mutation may suggest a more aggressive surgical approach. BRAF K601E mutation did not correlate with malignancy indexes.
- Published
- 2015
- Full Text
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