20 results on '"Ding, Shu-Qin"'
Search Results
2. Effect of morroniside on the transcriptome profiles of rat in injured spinal cords
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Shi, Yu-Jiao, Sheng, Wen-Jie, Xue, Meng-Tong, Duan, Fei-Xiang, Shen, Lin, Ding, Shu-Qin, Wang, Qi-Yi, Wang, Rui, Lü, He-Zuo, and Hu, Jian-Guo
- Published
- 2022
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3. Spatio-temporal expression of Hexokinase-3 in the injured female rat spinal cords
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Lin, Yu-Hong, Wu, Yan, Wang, Ying, Yao, Zong-Feng, Tang, Jie, Wang, Rui, Shen, Lin, Ding, Shu-Qin, Hu, Jian-Guo, and Lü, He-Zuo
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- 2018
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4. CRID3, a blocker of apoptosis associated speck like protein containing a card, ameliorates murine spinal cord injury by improving local immune microenvironment
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Chen, Yu-Qing, Wang, Sai-Nan, Shi, Yu-Jiao, Chen, Jing, Ding, Shu-Qin, Tang, Jie, Shen, Lin, Wang, Rui, Ding, Hai, Hu, Jian-Guo, and Lü, He-Zuo
- Published
- 2020
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5. Feature article: Neuroprotective effects of P7C3 against spinal cord injury in rats
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Duan, Fei-Xiang, Shi, Yu-Jiao, Chen, Jing, Ding, Shu-Qin, Wang, Feng-Chao, Tang, Jie, Wang, Rui, Shen, Lin, Xi, Jin, Qi, Qi, Lü, He-Zuo, and Hu, Jian-Guo
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Neurons ,Rats, Sprague-Dawley ,Oligodendroglia ,Neuroprotective Agents ,Spinal Cord ,Carbazoles ,Animals ,Female ,Motor Activity ,NAD ,Spinal Cord Injuries ,Original Research ,Rats - Abstract
Spinal cord injury (SCI), a serious neurological disease, has few therapeutic interventions. A small molecule, P7C3, has been confirmed to play a role in neuroprotection of some neurological diseases. But the effect of P7C3 on acute SCI has not been investigated. Here, we observed the therapeutic effect of P7C3 for alleviating the neurological damage by direct subcutaneous injection after SCI once daily for 7 or 14 days in a rat model. The locomotion and histopathological changes were evaluated. Our results demonstrated that P7C3 treatment contributed to functional recovery and tissue repair following SCI. Improved locomotor function with P7C3 treatment was correlated with increased survival of neurons and oligodendrocytes (OLs) and proliferation of OLs and their progenitors, which resulted in tissue repair and myelination in the injured SC. P7C3 treatment also restored the nicotinamide adenine dinucleotide level in the SC, which was reduced by SCI. These results suggest that P7C3 plays a role in improving neurological outcome following SCI. IMPACT STATEMENT: Spinal cord injury (SCI), a serious neurological disease, has few therapeutic interventions. This study confirms for the first time that P7C3 is conducive to functional recovery and tissue repair after SCI. P7C3 treatment can rescue the nicotinamide adenine dinucleotide level of the injured spinal cord, which results in more survival of neurons and oligodendrocytes (OLs) and proliferation of OLs and their progenitors, and thus increase myelination and tissue repair. This result indicates that P7C3 plays a role in improving neurological outcome following SCI.
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- 2019
6. Engraftment of human amniotic epithelial cells injected by different routes in the liver
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Zhang, Hai-yan, Piao, Zheng-fu, Ding, Shu-qin, Ren, Feng, Isao, Kamo, Norio, Sakuragawa, and Li, Ning
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- 2012
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7. Serum exosomal microRNA transcriptome profiling in subacute spinal cord injured rats.
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Ding, Shu-Qin, Chen, Yu-Qing, Chen, Jing, Wang, Sai-Nan, Duan, Fei-Xiang, Shi, Yu-Jiao, Hu, Jian-Guo, and Lü, He-Zuo
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EXOSOMES , *SPINAL cord , *PERIPHERAL circulation , *MICRORNA , *BODY fluids , *CENTRAL nervous system - Abstract
MicroRNAs (miRNAs) are involved in a series of pathology of spinal cord injury (SCI). Although, locally expressed miRNAs have advantages in studying the pathological mechanism, they cannot be used as biomarkers. The "free circulation" miRNAs can be used as biomarkers, but they have low concentration and poor stability in body fluids. Exosomal miRNAs in body fluids have many advantages comparing with free miRNAs. Therefore, we hypothesized that the specific miRNAs in the central nervous system might be transported to the peripheral circulation and concentrated in exosomes after injury. Using next-generation sequencing, miRNA profiles in serum exosomes of sham and subactue SCI rats were analyzed. The results showed that SCI can lead to changes of serum exosomal miRNAs. These changed miRNAs and their associated signaling pathways may explain the pathological mechanism of suacute SCI. More importantly, we found some valuable serum exosomal miRNAs for diagnosis and prognosis of SCI. • Serum exosomes of sham and subactue SCI rats were isolated and identified. • Serum exosomal microRNA transcriptome profiling in subacute spinal cord injured rats is investigated. • The changed miRNAs and their associated signaling pathways have been systematically determined. • Our results will provide a basis for the pathological mechanism, diagnosis and prognosis of SCI. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Effect of VX-765 on the transcriptome profile of mice spinal cords with acute injury.
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Chen, Jing, Chen, Yu-Qing, Wang, Sai-Nan, Duan, Fei-Xiang, Shi, Yu-Jiao, Ding, Shu-Qin, Hu, Jian-Guo, and Lü, He-Zuo
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SPINAL cord injuries ,FOCAL adhesions ,WESTERN immunoblotting ,MICE ,GENE ontology ,EXTRACELLULAR signal-regulated kinases - Abstract
Previous studies have shown that caspase-1 plays an important role in the acute inflammatory response of spinal cord injury (SCI). VX-765, a novel and irreversible caspase-1 inhibitor, has been reported to effectively intervene in inflammation. However, the effect of VX-765 on genome-wide transcription in acutely injured spinal cords remains unknown. Therefore, in the present study, RNA-sequencing (RNA-Seq) was used to analyze the effect of VX-765 on the local expression of gene transcription 8 h following injury. The differentially expressed genes (DEGs) underwent enrichment analysis of functions and pathways by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, respectively. Parallel analysis of western blot confirmed that VX-765 can effectively inhibit the expression and activation of caspase-1. RNA-Seq showed that VX-765 treatment resulted in 1,137 upregulated and 1,762 downregulated DEGs. These downregulated DEGs and their associated signaling pathways, such as focal adhesion, cytokine-cytokine receptor interaction, leukocyte transendothelial migration, extracellular matrix-receptor interaction, phosphatidylinositol 3-kinase-protein kinase B, Rap1 and hypoxia inducible factor-1 signaling pathway, are mainly associated with inflammatory response, local hypoxia, macrophage differentiation, adhesion migration and apoptosis of local cells. This suggests that the application of VX-765 in the acute phase can improve the local microenvironment of SCI by inhibiting caspase-1. However, whether VX-765 can be used as a therapeutic drug for SCI requires further exploration. The sequence data have been deposited into the Sequence Read Archive (https://www.ncbi.nlm.nih.gov/sra/PRJNA548970). [ABSTRACT FROM AUTHOR]
- Published
- 2020
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9. Anti-Osteoporotic Activity of an Edible Traditional Chinese Medicine Cistanche deserticola on Bone Metabolism of Ovariectomized Rats Through RANKL/RANK/TRAF6-Mediated Signaling Pathways.
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Zhang, Bo, Yang, Ling-Ling, Ding, Shu-Qin, Liu, Jing-Jing, Dong, Yan-Hong, Li, Yan-Ting, Li, Nan, Zhao, Xiao-Jun, Hu, Chang-Ling, Jiang, Yiping, and Ma, Xue-Qin
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CHINESE medicine ,BONE metabolism ,SODIUM carboxymethyl cellulose ,OSTEOPOROSIS in women ,TREATMENT effectiveness ,BONE density - Abstract
Given the limitations of existing therapeutic agents for treatment of postmenopausal osteoporosis, there still remains a need for more options with both efficacy and less adverse effects. Cistanche deserticola Y. C. Ma is known as a popular tonic herb traditionally used to treatment deficiency of kidney energy including muscle weakness in minority area of Asian counties. Based on the theory of "kidney dominate bone," an ovariectomized (OVX) rat model of postmenopausal osteoporosis was used to evaluate the therapeutic effect of C. deserticola extract (CDE) on bone loss. Forty eight female Sprague-Dawley rats, aged about 12 weeks, were randomly assigned into six groups including sham group orally administrated with 0.5% carboxymethyl cellulose sodium (CMC-Na) (sham), positive group treated with 1 mg/kg of estradiol valerate (EV), low, moderate, and high dosage groups orally administrated with 200, 400, and 800 mg/kg/day of CDE, respectively. After 3 months of continuous intervention, CDE exhibited significant anti-osteoporotic activity evidenced by the enhanced total bone mineral density, ameliorated bone microarchitecture; increased alkaline phosphatase activity; decreased deoxypyridinoline, cathepsin K, tartrate-resistant acid phosphatase, and malondialdehyde levels; whereas the body, uterus, and vagina weights in OVX rats were not influenced by CDE intervention. In addition, a seemed contradictory phenomenon on levels of calcium and phosphorus between OVX and sham rats were observed and elucidated. Mechanistically, CDE significantly down-regulated the levels of TRAF6, RANKL, RANK, NF-κB, IKKβ, NFAT2, and up-regulated the phosphatidylinositol 3-kinase (PI3K), AKT, osteoprotegerin, and c-Fos expressions, which implied CDE could suppress RANKL/RANK-induced activation of downstream NF-κB and PI3K/AKT pathways, and ultimately, preventing activity of the key osteoclastogenic proteins NFAT2 and c-Fos. All of the data suggested CDE possessed potential anti-osteoporotic activity and this effect was, at least in part, involved in modulation of RANKL/RANK/TRAF6-mediated NF-κB and PI3K/AKT signaling as well as c-Fos and NFAT2 levels. Therefore, CDE may represent a useful promising remedy candidate for treatment of postmenopausal osteoporosis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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10. Highlight article: Identification of serum exosomal microRNAs in acute spinal cord injured rats.
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Ding, Shu-Qin, Chen, Jing, Wang, Sai-Nan, Duan, Fei-Xiang, Chen, Yu-Qing, Shi, Yu-Jiao, Hu, Jian-Guo, and Lü, He-Zuo
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- 2019
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11. Effect of M2 macrophage adoptive transfer on transcriptome profile of injured spinal cords in rats.
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Chen, Jing, Wu, Yan, Duan, Fei-Xiang, Wang, Sai-Nan, Guo, Xue-Yan, Ding, Shu-Qin, Zhou, Ji-Hong, Hu, Jian-Guo, and Lü, He-Zuo
- Published
- 2019
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12. Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.
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Wu, Yan, Shen, Lin, Wang, Rui, Tang, Jie, Ding, Shu‐Qin, Wang, Sai‐Nan, Guo, Xue‐Yan, Hu, Jian‐Guo, and Lü, He‐Zuo
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- 2018
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13. Expression and Cellular Localization of IFITM1 in Normal and Injured Rat Spinal Cords.
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Wang, Ying, Lin, Yu-Hong, Wu, Yan, Yao, Zong-Feng, Tang, Jie, Shen, Lin, Wang, Rui, Ding, Shu-Qin, Hu, Jian-Guo, and Lü, He-Zuo
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SPINAL cord ,MEMBRANE proteins ,CYTOKINES ,MESSENGER RNA ,OLIGODENDROGLIA - Abstract
Interferon-induced transmembrane protein 1 (IFITM1) is a member of the IFITM family that is associated with some acute-phase cytokine-stimulated response. Recently, we demonstrated that IFITM1 was significantly upregulated in the injured spinal cords at the mRNA level. However, its expression and cellular localization at the protein level is still unclear. Here, a rat model of spinal cord injury (SCI) was performed to investigate the spatio-temporal expression of IFITM1 after SCI. IFITM1 mRNA and protein were assessed by quantitative reverse transcription-PCR and western blot, respectively. IHC was used to identify its cellular localization. We revealed that IFITM1 could be found in sham-opened spinal cords and gradually increased after SCI. It reached peak at 7 and 14 days postinjury (dpi) and still maintained at a relatively higher level at 28 dpi. IHC showed that IFITM1 expressed in GFAP
+ and APC+ cells in sham-opened spinal cords. After SCI, in addition to the above-mentioned cells, it could also be found in CD45+ and CD68+ cells, and its expression in CD45+ , CD68+ , and GFAP+ cells was increased significantly. These results demonstrate that IFITM1 is mainly expressed in astrocytes and oligodendroglia in normal spinal cords, and could rapidly increase in infiltrated leukocytes, activated microglia, and astrocytes after SCI. [ABSTRACT FROM AUTHOR]- Published
- 2018
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14. Transcriptome profile of rat genes in bone marrow-derived macrophages at different activation statuses by RNA-sequencing.
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Guo, Xue-Yan, Wang, Sai-Nan, Wu, Yan, Lin, Yu-Hong, Tang, Jie, Ding, Shu-Qin, Shen, Lin, Wang, Rui, Hu, Jian-Guo, and Lü, He-Zuo
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MACROPHAGE activation , *GENES , *RATS , *GENE expression , *MACROPHAGES , *BONES - Abstract
The underlying mechanisms of macrophage polarization have been detected by genome-wide transcriptome analysis in a variety of mammals. However, the transcriptome profile of rat genes in bone marrow-derived macrophages (BMM) at different activation statuses has not been reported. Therefore, we performed RNA-Sequencing to identify gene expression signatures of rat BMM polarized in vitro with different stimuli. The differentially expressed genes (DEGs) among unactivated (M0), classically activated pro-inflammatory (M1), and alternatively activated anti-inflammatory macrophages (M2) were analyzed by using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. In this study, not only we have identified the changes of global gene expression in rat M0, M1 and M2, but we have also made clear systematically the key genes and signaling pathways in the differentiation process of M0 to M1 and M2. These will provide a foundation for future researches of macrophage polarization. • The transcriptome profile of rat genes in bone marrow-derived macrophages at different activation statuses is investigated. • The global gene expression in rat M0, M1 and M2 has been described. • The key genes and signaling pathways in the differentiation process of M0 to M1 and M2 have been systematically determined. • Our results will provide a basis for future researches of macrophage polarization. [ABSTRACT FROM AUTHOR]
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- 2019
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15. The neuroprotective role of morroniside against spinal cord injury in female rats.
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Duan, Fei-Xiang, Shi, Yu-Jiao, Chen, Jing, Song, Xue, Shen, Lin, Qi, Qi, Ding, Shu-Qin, Wang, Qi-Yi, Wang, Rui, Lü, He-Zuo, and Hu, Jian-Guo
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SPINAL cord injuries , *SUPERIOR colliculus , *REACTIVE oxygen species , *RATS , *NEUROPROTECTIVE agents , *DISABILITIES - Abstract
Spinal cord injury (SCI) is a disabling condition that often leads to permanent neurological deficits without an effective treatment. Reactive oxygen species (ROS) produced during oxidative stress play a vital role in the pathogenesis following SCI. The antioxidant morroniside is the main active component of the Chinese medicine Cornus officinalis. In recent years, it has been reported that morroniside has therapeutic effects on damage to multiple organs mediated by oxidative damage, but the effect of morroniside on SCI has not been reported. The purpose of this study was therefore to assess the therapeutic effect of morroniside on SCI, and to identify its underlying mechanism by direct intragastric administration immediately after SCI. Our study showed that morroniside treatment improved the functional recovery of rats following SCI. This behavioral improvement was associated with the higher survival in neurons and oligodendrocytes following SCI, which increased the capacity of injured spinal cord (SC) to form myelin and repair tissue, eventually contributing to improved neurological outcome. Furthermore, our study found that oxygen free radicals increased and antioxidant enzyme activity decreased in the injured SC. Interestingly, morroniside treatment decreased oxygen free radical levels and increased antioxidant enzyme activities. Together, our results suggested that morroniside may be an effective treatment for improving outcomes following SCI, and that its antioxidant activity may be one of the mechanisms by which morroniside exerts neuroprotective effects on SCI. • Morroniside significantly promoted the histological recoveries of injured SC tissue and locomotor function in rats after SCI. • Morroniside inhibited oxidative stress and promoted the survival of neurons and OLs in injured SC. • Morroniside may be an effective treatment for improving outcomes following SCI. [ABSTRACT FROM AUTHOR]
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- 2021
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16. The polarization of microglia and infiltrated macrophages in the injured mice spinal cords: a dynamic analysis.
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Li JL, Fu GQ, Wang YY, Bian MM, Xu YM, Zhang L, Chen YQ, Zhang N, Ding SQ, Wang R, Fang R, Tang J, Hu JG, and Lü HZ
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- Female, Mice, Animals, Mice, Inbred C57BL, Macrophages pathology, Microglia pathology, Spinal Cord Injuries pathology
- Abstract
Background: Following spinal cord injury (SCI), a large number of peripheral monocytes infiltrate into the lesion area and differentiate into macrophages (Mø). These monocyte-derived Mø are very difficult to distinguish from the local activated microglia (MG). Therefore, the term Mø/MG are often used to define the infiltrated Mø and/or activated MG. It has been recognized that pro-inflammatory M1-type Mø/MG play "bad" roles in the SCI pathology. Our recent research showed that local M1 cells are mainly CD45
-/low CD68+ CD11b+ in the subacute stage of SCI. Thus, we speculated that the M1 cells in injured spinal cords mainly derived from MG rather than infiltrating Mø. So far, their dynamics following SCI are not yet entirely clear., Methods: Female C57BL/6 mice were used to establish SCI model, using an Infinite Horizon impactor with a 1.3 mm diameter rod and a 50 Kdynes force. Sham-operated (sham) mice only underwent laminectomy without contusion. Flow cytometry and immunohistofluorescence were combined to analyze the dynamic changes of polarized Mø and MG in the acute (1 day), subacute (3, 7 and 14 days) and chronic (21 and 28 days) phases of SCI., Results: The total Mø/MG gradually increased and peaked at 7 days post-injury (dpi), and maintained at high levels 14, 21 and 28 dpi. Most of the Mø/MG were activated, and the Mø increased significantly at 1 and 3 dpi. However, with the pathological process, activated MG increased nearly to 90% at 7, 14, 21 and 28 dpi. Both M1 and M2 Mø were increased significantly at 1 and 3 dpi. However, they decreased to very low levels from 7 to 28 dpi. On the contrary, the M2-type MG decreased significantly following SCI and maintained at a low level during the pathological process., Competing Interests: The authors declare that they have no competing interests., (© 2023 Li et al.)- Published
- 2023
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17. VX-765 reduces neuroinflammation after spinal cord injury in mice.
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Chen J, Chen YQ, Shi YJ, Ding SQ, Shen L, Wang R, Wang QY, Zha C, Ding H, Hu JG, and Lü HZ
- Abstract
Inflammation is a major cause of neuronal injury after spinal cord injury. We hypothesized that inhibiting caspase-1 activation may reduce neuroinflammation after spinal cord injury, thus producing a protective effect in the injured spinal cord. A mouse model of T9 contusive spinal cord injury was established using an Infinite Horizon Impactor, and VX-765, a selective inhibitor of caspase-1, was administered for 7 successive days after spinal cord injury. The results showed that: (1) VX-765 inhibited spinal cord injury-induced caspase-1 activation and interleukin-1β and interleukin-18 secretion. (2) After spinal cord injury, an increase in M1 cells mainly came from local microglia rather than infiltrating macrophages. (3) Pro-inflammatory Th1Th17 cells were predominant in the Th subsets. VX-765 suppressed total macrophage infiltration, M1 macrophages/microglia, Th1 and Th1Th17 subset differentiation, and cytotoxic T cells activation; increased M2 microglia; and promoted Th2 and Treg differentiation. (4) VX-765 reduced the fibrotic area, promoted white matter myelination, alleviated motor neuron injury, and improved functional recovery. These findings suggest that VX-765 can reduce neuroinflammation and improve nerve function recovery after spinal cord injury by inhibiting caspase-1/interleukin-1β/interleukin-18. This may be a potential strategy for treating spinal cord injury. This study was approved by the Animal Care Ethics Committee of Bengbu Medical College (approval No. 2017-037) on February 23, 2017., Competing Interests: None
- Published
- 2021
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18. Neuroprotective effects of P7C3 against spinal cord injury in rats.
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Duan FX, Shi YJ, Chen J, Ding SQ, Wang FC, Tang J, Wang R, Shen L, Xi J, Qi Q, Lü HZ, and Hu JG
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- Animals, Female, Motor Activity drug effects, NAD metabolism, Neurons drug effects, Oligodendroglia drug effects, Rats, Rats, Sprague-Dawley, Spinal Cord drug effects, Spinal Cord metabolism, Carbazoles therapeutic use, Neuroprotective Agents therapeutic use, Spinal Cord Injuries drug therapy
- Published
- 2019
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19. Identification of serum exosomal microRNAs in acute spinal cord injured rats.
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Ding SQ, Chen J, Wang SN, Duan FX, Chen YQ, Shi YJ, Hu JG, and Lü HZ
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- Acute Disease, Animals, Down-Regulation genetics, Exosomes ultrastructure, Female, Gene Expression Profiling, Gene Ontology, MicroRNAs genetics, RNA, Untranslated genetics, RNA, Untranslated metabolism, Rats, Sprague-Dawley, Reproducibility of Results, Spinal Cord pathology, Up-Regulation genetics, Exosomes metabolism, MicroRNAs blood, Spinal Cord Injuries blood, Spinal Cord Injuries genetics
- Published
- 2019
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20. Expression and localization of absent in melanoma 2 in the injured spinal cord.
- Author
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Wang SN, Guo XY, Tang J, Ding SQ, Shen L, Wang R, Ma SF, Hu JG, and Lü HZ
- Abstract
In traumatic brain injury, absent in melanoma 2 (AIM2) has been demonstrated to be involved in pyroptotic neuronal cell death. Although the pathophysiological mechanism of spinal cord injury is similar to that of brain injury, the expression and cellular localization of AIM2 after spinal cord injury is still not very clear. In the present study, we used a rat model of T9 spinal cord contusive injury, produced using the weight drop method. The rats were randomly divided into 1-hour, 6-hour, 1-day, 3-day and 6-day (post-injury time points) groups. Sham-operated rats only received laminectomy at T9 without contusive injury. Western blot assay revealed that the expression levels of AIM2 were not significantly different among the 1-hour, 6-hour and 1-day groups. The expression levels of AIM2 were markedly higher in the 1-hour, 6-hour and 1-day groups compared with the sham, 3-day and 7-day groups. Double immunofluorescence staining demonstrated that AIM2 was expressed by NeuN
+ (neurons), GFAP+ (astrocytes), CNPase+ (oligodendrocytes) and CD11b+ (microglia) cells in the sham-operated spinal cord. In rats with spinal cord injury, AIM2 was also found in CD45+ (leukocytes) and CD68+ (activated microglia/macrophages) cells in the spinal cord at all time points. These findings indicate that AIM2 is mainly expressed in neurons, astrocytes, microglia and oligodendrocytes in the normal spinal cord, and that after spinal cord injury, its expression increases because of the infiltration of leukocytes and the activation of astrocytes and microglia/macrophages., Competing Interests: None- Published
- 2019
- Full Text
- View/download PDF
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