1. Novel trisaccharide based phospholipids as immunomodulators
- Author
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Sampath Kumar Halmuthur Mahabalarao, Mallika Alvala, Sylviane Muller, Debabrata Bhunia, Sridhar Goud Nerella, Srinivasa Reddy Bonam, Biotechnologie et signalisation cellulaire (BSC), and Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS)
- Subjects
0301 basic medicine ,Cytotoxicity, Immunologic ,Mannosides ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Ovalbumin ,Immunology ,Receptors, Cell Surface ,Lymphocyte Activation ,Antibodies ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Adjuvants, Immunologic ,In vivo ,Imidoesters ,Immunology and Allergy ,Animals ,Immunologic Factors ,Lectins, C-Type ,Phosphatidylinositol ,Phosphorylation ,Cells, Cultured ,Phospholipids ,Pharmacology ,Antigen Presentation ,Innate immune system ,biology ,Chemistry ,Dendritic Cells ,In vitro ,3. Good health ,Molecular Docking Simulation ,030104 developmental biology ,Biochemistry ,Docking (molecular) ,030220 oncology & carcinogenesis ,biology.protein ,Cytokines ,Cell Adhesion Molecules ,Trisaccharides ,Immunopotentiation - Abstract
A focused library of novel mannosylated glycophospholipids was synthesized employing imidate coupling and H-phosphate phosphorylation methods. All novel glycophospholipids were evaluated for their receptor interactions by molecular docking studies. Docking studies revealed dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) specific interaction of the glycophospholipid ligand P4 acts, which was further confirmed by in vitro DC-SIGN expression on monocyte-derived dendritic cells (MoDCs). Further, in vitro and in vivo immunomodulatory activity among the six compounds ( P1 - P6 ) examined, compound P4 displayed good immunopotentiation and adjuvant properties as indicated by the induced cytokine expression and enhanced ovalbumin (OVA) specific antibody (IgG) titers. Phosphatidylinositol mannosides (PIMs) analogues in the present study enforced the immunomodulatory properties, truncating parent PIMs or tailor-made of PIMs may bring the novel efficacious molecules, which will be useful in vaccine preparation against different diseases.
- Published
- 2019
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