Background: Grassland plays an important role in the ecosystem, but overgrazing harms the grassland system in many places. Grazing prohibition is an effective method to restore grassland ecosystems, and it plays a great role in realizing the sustainable development of grassland systems. Therefore, it is necessary to carry out research on the influence of regional grazing prohibition on the physical and chemical properties of different grassland systems., Methods: In Potatso National Park, Southwest China, we selected experimental plots in the artificial grazing meadow area to study the effects of grazing prohibition on plant and soil indexes in subalpine meadows and swamp meadows. We investigated the biomass and species diversity of grazing prohibition treatment and grazing treatment plots and sampled and tested the soil index. The variation percentage was used to remove the original heterogeneity and yearly variation, allowing us to compare differences in plant index and soil index values between grazing prohibition and grazing treatments., Results: Grazing prohibition increased the aboveground biomass, total biomass, total meadow coverage, average height, richness index, Shannon diversity index and evenness index and reduced the belowground biomass and root/shoot ratio in the subalpine meadow and swamp meadow. Additionally, grazing prohibition reduced the pH and soil bulk density and increased the soil total carbon, soil organic carbon, soil total nitrogen, soil hydrolyzable nitrogen, soil total phosphorus and soil available phosphorus in the subalpine meadow and swamp meadow. Nonmetric multidimensional scaling (NMDS) analysis showed that both plant indexes and soil indexes were significantly different between grazing and grazing prohibition treatments and between meadow types. Short-term grazing prohibition had a great impact on improving the fertility of meadow soil in the study area. We suggest that long-term and extensive research should be carried out to promote the restoration and sustainable development of regional grassland systems., Competing Interests: The authors declare there are no competing interests., (©2021 Zhu et al.)