125 results on '"DAL, Tuba"'
Search Results
2. Outbreak caused by multidrug-resistant OXA-48 and NDM-1 producing Klebsiella pneumoniae in the intensive care unit of a cancer hospital.
- Author
-
Süzük Yıldız, Serap, Tavukcu, Esra, Şahin, Sevgi, İskender, Gülşen, Ünaldı, Özlem, Demirhan, Buket, Avşar, Zuhal, Orman, Elif Özgür, Mumcuoğlu, İpek, and Dal, Tuba
- Subjects
PULSED-field gel electrophoresis ,CANCER hospitals ,KLEBSIELLA pneumoniae ,INFECTION control ,MEDICAL care - Abstract
We report a nosocomial outbreak caused by a multidrug-resistant carbapenemase-producing Klebsiella pneumoniae (MDRCP Kp), that was detected in six patients admitted to the medical intensive care unit between 20th of December 2023 and 15th of January 2024 in Ankara, Turkey. The investigation of this outbreak was started on 29th of December 2023. During the outbreak 11 samples were collected from the six patients with MDRCP Kp. Pulsed-field gel electrophoresis (PFGE) was performed to determine the genetic relatedness and clonality of MDRCP Kp strains. MDRCP Kp was isolated in the tracheal aspiration culture, blood, urine, and screening samples. Five patients with MDRCP Kp colonization developed healthcare-associated infection. In one patient MDRCP Kp was isolated from tracheal aspirate and the screening cultures were considered as colonization not infection. PFGE analysis revealed that all isolates belonged to the same K. pneumoniae clone. MDRCP Kp strain of this outbreak exhibited multidrug resistance and co-produced OXA-48 and NDM-1. This outbreak ended after application of strict infection control measures. An outbreak of MDRCP Kp can occur in hospitals, especially in the intensive care units; thus, it should be detected early by infection control teams. A strong collaboration between infection control team and microbiology laboratory is essential to cope with MDR bacterial outbreaks in hospitals. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Antisense peptide nucleic acids againstftsZ andefaA genes inhibit growth and biofilm formation of Enterococcusfaecalis
- Author
-
Narenji, Hanar, Teymournejad, Omid, Rezaee, Mohammad Ahangarzadeh, Taghizadeh, Sepehr, Mehramuz, Bahareh, Aghazadeh, Mohammad, Asgharzadeh, Mohammad, Madhi, Masoumeh, Gholizadeh, Pourya, Ganbarov, Khudaverdi, Yousefi, Mehdi, Pakravan, Asrin, Dal, Tuba, Ahmadi, Raman, and Samadi Kafil, Hossein
- Published
- 2020
- Full Text
- View/download PDF
4. Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis
- Author
-
Zeybek, Hasan, Acikgoz, Ziya Cibali, Dal, Tuba, and Durmaz, Rıza
- Published
- 2020
- Full Text
- View/download PDF
5. Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis
- Author
-
Dal, Tuba, Kara, Soner Sertan, Cikman, Aytekin, Balkan, Cigdem Eda, Acıkgoz, Ziya Cibali, Zeybek, Hasan, Uslu, Hakan, and Durmaz, Rıza
- Published
- 2019
- Full Text
- View/download PDF
6. Effect of climate change on nature and human health with a special focus on infectious diseases in the Mediterranean region.
- Author
-
Dal, Tuba, Ramli, Imen, and Garaizar, Javier
- Subjects
- *
THALASSEMIA , *COMMUNICABLE diseases , *CLIMATE change - Abstract
Introduction: In this short review, the effect of climate change on nature and human health with a special focus on infectious diseases in the Mediterranean region is discussed. This research is a part of the Mediterranean Convention of Human Rights project, which is an organizational work on human rights issues that was established in cooperation with civil society and the national authorities of the Mediterranean Region. Methodology: Previously published data were collected by retrieving published literature from PubMed, Google Scholar, and Web of Science using "climate change", "the Mediterranean region", "infections in Mediterranean Region", "infectious diseases", "biodiversity", and "the Mediterranean Sea" as keywords. The collected data were then evaluated and reviewed. The recommendations and guidelines were analysed by the preferred reporting items for systematic reviews and meta-analyses (PRISMA). Conclusions: The Mediterranean region presents a typical example witnessing a dramatic change in climate events and their adverse impact on biodiversity, ecosystems and public health are multiple. This negative impact is in part due to the geographical particularities, and sociocultural and geopolitical conflicts that are progressively worsening the burden of climate change. While most of these changes cannot be totally avoided, many of the health risks related to climate change could be monitored. This can be done by establishing health systems with policies to reduce and prevent the risks of infectious diseases and to recover and support the affected areas, which may identify priority and management of highrisk events. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
7. Investigation of Streptococcus agalactiae serotypes in clinical samples
- Author
-
Arabacı, Çiğdem, Dal, Tuba, Ekin, İsmail Hakkı, and Babacan, Funda
- Subjects
serotype ,Infection ,colonization ,Streptococcus agalactiae - Abstract
Objective: A key contributor to neonatal early-onset sepsis, postpartum maternal sepsis, and infant late-onset invasive infections is Streptococcus agalactiae (group B Streptococcus; GBS). GBS may also result in bone and joint infections, bacteremia, endocarditis, pneumonia, and infections of the skin and soft tissues in adults. The bacterial polysaccharide capsule may distinguish between ten GBS serotypes (Ia, Ib, and II–IX). In order to reduce maternal colonization and prevent transmission to neonates, GBS capsular polysaccharide vaccines have been investigated. We aimed to detect the differences of the polysaccharide capsules among GBS isolates. Methods: This study used GBS isolates from several clinical specimens in the Microbiology Laboratory at Marmara University Hospital in Istanbul. Fifty of the isolates were colonized (37 genital site, 13 throat swab), and 50 of the isolates were infectious (33 urine, 7 blood, 2 respiratory specimens, and 4 wound swab specimens, 4 sterile body fluids). The serotypes of GBS isolates were determined by detecting visible agglutination when specific GBS capsule antigens reacted with serotype monospecific antibodies. Results: Colonized strain distribution values for serotype Ia, serotype II, serotype III, serotype Ic, serotype R, serotype V, serotype IV, serotype II/R, serotype III/R, and non-serotyping stains were 12 (24%), 11 (22%), 7 (14%), 3 (6%), 3 (6%) , 2 (4%), 1 (2%), 2 (4%), 1 (2%), 8 (16%) respectively. Causative agents distribution values for serotype II, serotype Ia, serotype III, serotype R, serotypes IV and serotype VIII, II / R, III / R, and non-serotyping stains were 15 (30%), 13 (26%), 10 (20%), 2 (4%), 1 (2%), 1 (2%), 3 (6%), 2 (4%) and 2 (4%) respectively. Conclusion: Serotype Ia, serotype II, and serotype III were the common serotypes in our clinical isolates but molecular-based studies associated with GBS population are needed in our country.
- Published
- 2023
- Full Text
- View/download PDF
8. Molecular epidemiological investigation of carbapenem resistant Klebsiella pneumoniae isolated from intensive care unit patients of six geographical regions of Turkey.
- Author
-
Köse, Şükran, Dal, Tuba, Çetinkaya, Rıza Aytaç, Ar, Oğuz, Yenilmez, Ercan, Temel, Esra Nurlu, Çetin, Emel Sesli, Arabacı, Çiğdem, Büyüktuna, Seyit Ali, Hasbek, Mürşit, Külahlıoğlu, Neslihan, Müderris, Tuba, Kaynar, Teoman, Sarı, Tuğba, Yalçın, Ata Nevzat, Ak, Kenan, Yıldırım, Berivan Tunca, Şahin, Merve, and Durmaz, Rıza
- Subjects
- *
INTENSIVE care patients , *KLEBSIELLA pneumoniae , *MICROBIAL sensitivity tests , *COLISTIN , *INFECTIOUS disease transmission - Abstract
Introduction: Klebsiella pneumonia causes serious infections in hospitalized patients. In recent years, carbapenem-resistant infections increased in the world. The molecular epidemiological investigation of carbapenem-resistant K. pneumoniae isolates was aimed in this study. Methodology: Fifty carbapenem-resistant K. pneumoniae isolates from six geographical regions of Turkey between September 2019-2020 were included in the study. The disk diffusion method was used for the antibiotic susceptibility testing. The microdilution confirmed colistin susceptibility. Genetic diversity was investigated by MLST (Multi-Locus Sequence Typing). Results: The resistance rates were as follows: 49 (98%) for meropenem, 47 (94%) imipenem, 50 (100%) ertapenem, 30 (60%) colistin and amoxicillin-clavulanate, 49 (98%) ceftriaxone, 48 (96%) cefepime, 50 (100%) piperacillin-tazobactam, 47 (94%) ciprofloxacin, 40 (80%) amikacin, 37 (74%) gentamicin. An isolate resistant to colistin by disk diffusion was found as susceptible to microdilution. ST 2096 was the most common (n:16) sequence type by MLST. ST 101 (n:7), ST14 (n:6), ST 147 and ST 15 (n:4), ST391 (n:3), ST 377 and ST16 (n:2), ST22, ST 307, ST 985, ST 336, ST 345, and ST 3681 (n:1) were classified in other isolates. In İstanbul and Ankara ST2096 was common. Among Turkey isolates, the most common clonal complexes (CC) were CC14 (n:26) and CC11 (n = 7). Conclusions: In Turkey, a polyclonal population of CC14 throughout the country and inter-hospital spread were indicated. The use of molecular typing tools will highlight understanding the transmission dynamics. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
9. Bacterial colonization due to increased nurse workload in an intensive care unit
- Author
-
Aycan, Ilker Onguc, Celen, Mustafa Kemal, Yilmaz, Ayhan, Almaz, Mehmet Selim, Dal, Tuba, Celik, Yusuf, and Bolat, Esef
- Published
- 2015
- Full Text
- View/download PDF
10. First year real life experience with tenofovir alafenamide fumarate: The pythagorean cohort.
- Author
-
Karasahin, Omer, Kalkan, Irem Akdemir, Dal, Tuba, Toplu, Sibel Altunisik, Harputluoglu, Murat, Mete, Ayse Ozlem, Komur, Suheyla, Sarigul, Figen, Yildiz, Yesim, Esmer, Fatih, Kandemir, Ozlem, Nazik, Selcuk, Inan, Dilara, Akgul, Fethiye, Kaya, Safak, Tunc, Nurettin, Bayindir, Yasar, Balin, Safak Ozer, Tasova, Yesim, and Aktar, Fesih
- Subjects
CHRONIC hepatitis B ,TENOFOVIR ,LIVER function tests ,MORTALITY ,KIDNEY function tests - Abstract
Background and Aim: In chronic hepatitis B infection, antiviral therapy significantly reduces the incidence of complications. This study aimed to present real-life 12-month effectiveness and safety data for TAF. Materials and Methods: This Pythagoras Retrospective Cohort Study included patients from 14 centers in Turkiye. The study presents 12-month results of 480 patients treated with TAF as initial therapy or after switching from another antiviral drug. Results: The study shows treatment of about 78.1% patients with at least one antiviral agent (90.6% tenofovir disoproxil [TDF]). The rate of undetectable HBV DNA increased in both treatment-experienced and naive patients. In TDF-experienced patients, the rate of alanine transaminase (ALT) normalization increased slightly (1.6%) within 12 months, but the change was not statistically significant (p=0.766). Younger age, low albumin, and high body mass index and cholesterol were identified as risk factors for abnormal ALT after 12 months, but no linear relationship was detected. In TDF-experienced patients, renal and bone function indicators showed significant improvement three months after the transition to TAF and remained stable for 12 months. Conclusion: Real-life data demonstrated effective virological and biochemical responses with TAF therapy. After switching to TAF treatment, gains in kidney and bone functions were achieved in the early period. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
11. A 4-Year Surveillance of Device-associated Nosocomial Infections in a Neonatal Intensive Care Unit
- Author
-
Tekin, Recep, Dal, Tuba, Pirinccioglu, Habibe, and Erisir Oygucu, Seyhan
- Published
- 2013
- Full Text
- View/download PDF
12. COVID-19 vaccine hesitancy in Turkey: A systematic review and meta-analysis.
- Author
-
Gulle, Bugra Taygun, Oren, Meryem Merve, and Dal, Tuba
- Abstract
This systematic review and meta-analysis aims to estimate the prevalence of coronavirus disease 2019 (COVID-19) vaccine hesitancy in Turkey, which can aid future health policies and strategies. A comprehensive search was conducted on various databases using keywords related to COVID-19 vaccine hesitancy in Turkey. Quality assessment was performed using Joanna Briggs Institute (JBI) checklist for prevalence studies. Data extraction was conducted. The random effect model (DerSimonian and Laird method) was used in pooled prevalence data analysis (95% confidence interval [CI]). A total of 1,072 articles were identified. After removing duplicates and excluding articles, 61 articles remained for bias assessment. Among these, 19 articles with low risk of bias were included in the review and meta-analysis. Total population included in the analysis was 15,164, vaccine hesitancy was 30.5% (95% Cl: 24.3–36.8%). Prevalence of the vaccine hesitancy was found to be 39.8% (95% Cl: 31.4–48.2%) in studies conducted before the initiation of vaccination, while in studies conducted after the commencement of vaccination, hesitancy was 20.4% (95% Cl: 12.9–28%). We suggest conducting high-quality studies in different populations to understand the level of vaccine hesitancy, as many of the previous studies have mainly focused on healthcare workers and students, and rest were community-based studies, which have generally shown high bias. Also, we suggest that early vaccination can reduce vaccine hesitancy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
13. Molecular investigation of carbapenem and colistin resistance mechanisms in klebsiella pneumoniae bloodstream isolates
- Author
-
Genişel, Neslihan, Özcan, Nida, Gül, Kadri, Akpolat, Nezahat, Atmaca, Selahattin, Kenar, Levent, Altanlar, Nurten, Dal, Tuba, Dicle Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bililmleri Bölümü, Genişel, Neslihan, Özcan, Nida, Gül, Kadri, Akpolat, Nezahat, and Atmaca, Selahattin
- Subjects
Carbapenemase ,Klebsiella pneumoniae ,Sıvı mikrodilüsyon ,Multipleks PZR ,Bla OXA-48 ,Karbapenemaz ,Multiplex PCR ,Mcr-1 ,Broth microdilution - Abstract
Carbapenem-Resistant Klebsiella pneumoniae (CRKp) infections are worrying health problems due to decreasing treatment options. This study investigates the carbapenemase (OXA-23,24, 48, 51, 55, 58, KPC, NDM-1, VIM, IMP) and mcr-1 genes of the CRKps isolates A total of 33 CRKp isolates isolated from patient blood samples from the Dicle University Medical Faculty Hospital, intensive care units (ICUs) between February 2020 and June 2020, were included in the study. The presence of carbapenemase encoding genes -including all CRKp isolates, bla OXA-23, 24, 48, 58, bla KPC, blaNDM-1, bla VIM, bla IMP- were investigated by multiplex Polymerase Chain Reaction (PCR). CRKp isolates were tested for mcr-1 gene and bla OXA-51, bla OXA-55 genes by monoplex PCR. All CRKp isolates studied with Kirby Bauer Disc Diffusion Method (DDM) (100%) were resistant to ertapenem, 9 (27.27%) resistant to imipenem, and 23 (69.70%) were resistant to meropenem. 20 (60.61%) of the isolates were found resistant to colistin. bla OXA-48, bla NDM-1 and bla OXA-24 genes were found in 75.76% (n = 25), 6.06% (n = 2) and 3.03% (n = 1) isolates, respectively. Both bla OXA-48 and bla NDM-1 genes were detected in two (6.06%) isolates and mcr-1 gene in 16 (48.48%) isolates. While the mean hospitalization was 20.3 days in 13 patients with a colistin minimum inhibitory concentration (MIC) of 2 μg/ml, it was 33.9 days in 20 patients with a colistin MIC of > 2 μg/ml. The average length of stay in the hospital was 21.8 days in mcr-1 negative patients and 35.7 days in mcr-1 positive patients. Carbapenemase and mcr-1 positivities were found at dramatically high rates in Diyarbakır, Turkey. It was indicated that plasmid-mediated antimicrobial resistance in Kp isolates was problematic. Each hospital should monitor the colistin and carbapenem resistance mechanisms by molecular methods. Colistin resistance should be confirmed by the broth microdilution method (BMD). Karbapenem Dirençli Klebsiella pneumoniae (KDKp) enfeksiyonları azalan tedavi seçenekleri nedeniyle endişe verici sağlık sorunları oluşturmaktadır. Bu çalışmada, KDKp izolatlarının karbapenemaz (OXA-23,24, 48, 51, 55, 58, KPC, NDM-1, VIM, IMP) ve mcr-1 genleri araştırılmaktadır. Dicle Üniversitesi Tıp Fakültesi Hastanesi yoğun bakım ünitelerinden (YBÜ) Şubat 2020 ile Haziran 2020 tarihleri arasında alınan hasta kan örneklerinden izole edilen toplam 33 KDKp izolatı çalışmaya dahil edildi. Tüm KDKp izolatları, bla OXA-23, 24, 48, 58, bla KPC, blaNDM-1, bla VIM, bla IMP dahil olmak üzere karbapenemaz kodlayan genlerin varlığı multipleks Polimeraz Zincir Reaksiyonu (PZR) ile araştırıldı. KDKp izolatları monoplex PZR ile mcr- 1 geni ve bla OXA-51, bla OXA-55 genleri için test edildi. Kirby Bauer Disk Difüzyon Test (DDT) (%100) ile çalışılan tüm KDKp izolatları ertapenem’e dirençliydi; 9’u (%2.27) imipeneme, 23’ü (%69.70) meropeneme dirençliydi. İzolatların 20’si (%60,61) kolistine dirençli bulundu. bla OXA-48, bla NDM-1 ve bla OXA-24 genleri sırasıyla %75.76 (n=25), %6.06 (n=2) ve %3.03 (n=1) izolatında bulundu. İki (%6.06) izolatta hem bla OXA-48 hem de bla NDM-1 genleri, 16 (%48,48) izolatta mcr-1 geni saptandı. Kolistin minimum inhibitör konsantrasyonu (MİK) değeri 2 μg/ml olan 13 hastada ortalama yatış süresi 20.3 gün iken, kolistin MİK değeri > 2 μg/ml olan 20 hastada 33.9 gündü. Hastanede ortalama kalış süresi mcr-1 negatif hastalarda 21.8 gün, mcr-1 pozitif hastalarda 35.7 gündü. Karbapenemaz ve mcr- 1 pozitiflikleri Diyarbakır, Türkiye’de çarpıcı biçimde yüksek oranlarda bulundu. Kp izolatlarında plazmit aracılı antimikrobiyal direncin sorunlu olduğu belirtildi. Her hastane moleküler yöntemlerle kolistin ve karbapenem direnç mekanizmalarını izlemelidir. Kolistin direnci sıvı mikrodilüsyon yöntemi ile doğrulanmalıdır.
- Published
- 2021
14. Real-life experience of tenofovir alafenamide fumarate: The pythagorean cohort
- Author
-
Karaşahin, Ömer, Kalkan, İrem Akdemir, Dal, Tuba, Toplu, Sibel Altunışık, Harputoğlu, Murat, Mete, Ayse Özlem, Aktar, Fesih, Demir, Yakup, Çelen, Mustafa Kemal, Dicle Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Çocuk Sağlığı ve Hastalıkları Ana Bilim Dalı, Aktar, Fesih, Demir, Yakup, and Çelen, Mustafa Kemal
- Abstract
Real-life experience of tenofovir alafenamide fumarate: The pythagorean cohort
- Published
- 2021
15. Real-life data for tenofovir alafenamide fumarate treatment of hepatitis B: The pythagoras cohort
- Author
-
Tasova, Yesim, Oren, Meryem Merve, Akar, Fesih, Karasahin, Omer, Esmer, Fatih, AKDEMİR KALKAN, İREM, Tunc, Nurettin, Balin, Safak Ozer, Kandemir, Ozlem, Bayindir, Yasar, Inan, Dilara, Yildiz, YEŞİM, Sarigul, Figen, KÖMÜR, SÜHEYLA, Mete, Ayse Ozlem, Dal, Tuba, Demir, Yakup, Nazik, Selcuk, Akgul, Fethiye, Celen, Mustafa K., ALTUNIŞIK TOPLU, SİBEL, Kaya, Safak, Harputoglu, Murat, Ayhan, Merve, Dicle Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Çocuk Sağlığı ve Hastalıkları Ana Bilim Dalı, Aktar, Fesih, and Çelen, Mustafa Kemal
- Subjects
Tenofovir Alafenamide ,Real life ,Bone mineral density ,virus diseases ,Chronic Hepatitis B ,Glomerular filtration rate ,Hepatitis B ,Tenofovir Disoproxil - Abstract
WOS:000635211600001 Background: Chronic hepatitis B (CHB) is a viral infection that can result in life-threatening conditions, such as hepatocellular carcinoma and cirrhosis. Tenofovir, which is used for the treatment of CHB, is a nucleotide analog that inhibits HBV-DNApolymerase and has two formulations: disoproxil and alafenamide. In contrast to tenofovir disoproxil fumarate (TDF), tenofovir alafenamide fumarate (TAF) penetrates the whole hepatocyte without being eliminated due to its longer plasma half-life and greater plasma stability. As a result, side effects such as proximal renal tubulopathy and loss of bone density are less common in the treatment of TAF and have similar efficacy to TDF. Objectives: The purpose of the study was to evaluate the effectiveness and reliability of TAF using real-life data. Methods: This retrospective cohort study was carried out in secondary or tertiary healthcare centers in southern Turkey. A total of 480 patients aged 18 years and older were administered TAF for an appropriate indication by the infectious diseases and gastroenterology clinics of the healthcare centers participating in this study. The data collected at t = 0, t =3, and t = 6 months of treatment were analyzed. The chi-square, Mann-Whitney U, Friedman, Wilcoxon, Cochran's Q, and McNemar's tests were used. Results: The mean age of the patients was 47.40 +/- 14.5, and 327 of them (68.1%) were male. A total of 78.1% of the 480 patients who underwent the TAF treatment had previous antiviral therapy experience (TDF, n =340; 70.8 %), and 21.9% were treatment-naive. The most common reasons for the initiation of TAF treatment were the use of drugs affecting bone mineral density (BMD) (42.9%) and osteoporosis (22.3%). Patients who had taken TDF experienced a significant improvement in glomerular filtration rate (GFR), hip and spine T-scores, and phosphorus levels from t = 0 months tot = 6 months after switching to TAF (P < 0.05). For this group, no statistically significant difference was observed concerning LDL and cholesterol levels from t= 0 months to t = 6 months. Side effects were reported by 5.7% of patients in the third month and 7.1% in the sixth month, with the most common side effect being hair loss (1%). Conclusions: TAF was found to be an effective and safe alternative to TDF with lower incidences of its long-term effects, such as nephrotoxicity and decreased bone density.
- Published
- 2021
16. Tıp Fakültesi Öğrencilerinin Pandemi Döneminde Meslekleri ile İlgili Kaygılarının İncelenmesi
- Author
-
KUTLUHAN, Ahmet, ÇALIK VAR, Esra, YÜKSEL, Selcen, DAL, Tuba, ŞEN, Mehmet Can, and ERSOY, Reyhan
- Subjects
Kaygı,Tıp Eğitimi,Covid-19,Pandemi ,Anxiety,Medical Education,Covid-19,Pandemic ,Health Care Sciences and Services ,Sağlık Bilimleri ve Hizmetleri - Abstract
Giriş: Sağlık sistemi Covid-19 pandemisi sürecinde derinden etkilenmiştir. Sağlık çalışanları yüksek risk altında hizmet vermektedir. Bu dönemde tıp öğrencileri uzaktan eğitimlerine devam etmektedirler. Bu çalışmada, tıp fakültesi öğrencilerinin kendi sağlıklarının yanı sıra meslekleri hakkındaki kaygıları ve tıp fakültesine başlama kararları ile ilgili düşüncelerinin incelenmesi amaçlanmıştır. Yöntem: Bu çalışma 10-17 Mayıs 2020 tarihleri arasında Ankara Yıldırım Beyazıt Üniversitesi Tıp Fakültesi'nde yapılmıştır. Demografik bilgiler, mesleki kaygı, sağlık kaygısı ve sağlık biliş düzeyi hakkında soruları içeren çevrimiçi anket 2281 öğrenciye gönderilmiştir. Veriler elverişli örneklem yöntemi ile elde edilmiştir. Verilerin dağılımı medyan (minimum-maksimum) kullanılarak sunulmuştur. Ankete verilen yanıtlar arasındaki ilişkiyi değerlendirmek için çift serili korelasyon katsayısı, ki-kare testi ve polikorik korelasyon katsayısı kullanıldı. Bulgular: Anketi toplam 885 öğrenci tamamlamıştır. Ortalama yaş 21.47 (± 2.62) olarak bulunmuştur. Çalışmaya katılanların 545'i (%61,9) kadındı. 809 öğrenci (%91,8) tıp fakültesini kendi isteğiyle, 72'si (%8,2) ise istemeyerek seçtiğini belirtmiştir. Tıp fakültesini istemeyerek seçenlerin meslek değişikliği yapma isteği yüksek bulunmuştur. Uzaktan eğitim 168 (%19,2) öğrenciye göre yeterli bulunurken 435 öğrenci için kısmen yeterli olarak değerlendirilmiştir. Öğrencilerin okumakta olduğu yarıyıl arttıkça mesleki kaygıları, mesleklerinin avantaj ve dezavantajlarına ilişkin farkındalıkları, sağlık sistemine güvenleri, farklı mesleki kaynakları gözden geçirme yetenekleri artmıştır. Kız öğrencilerin, erkek öğrencilere göre mesleki ve genel sağlıkları ile daha fazla endişe duyduğu bulunmuştur. Sonuç: Pandeminin tıp öğrencilerinin meslek seçimi ve kaygı düzeyleri üzerinde etkisi olmasına rağmen, genel olarak bu etkiler çok yüksek bulunmamıştır. Mesleki kaygı, mesleğe ilişkin farkındalık, sağlık sistemine güven, farklı mesleki kaynakları gözden geçirme becerisi okumakta oldukları yarıyıl ile ilişkiliydi. Uzaktan eğitim ile ilgili iyileştirilmeler yapılmadır. Pandemi sürecinde öğrencilere psikolojik destek sağlanması faydalı olabilir. Ayrıca sonuçlar, araştırmanın yapıldığı zaman ve pandeminin kısa sürede sona ereceği beklentisi nedeniyle iyimser olarak cevaplandırılmış olabileceği göz önüne alınarak değerlendirilmelidir., Aim: The Covid-19 pandemic has affected healthcare system deeply. The healthcare workers served at high risk. In this period, medical students continued their education remotely. This study was aimed to evaluate the concerns of the medical students about their own health as well as their profession and thoughts about their decision to start medical school. Methods: This study was conducted in Ankara Yildirim Beyazit University, Faculty of Medicine between 10-17 May 2020. The online questionnaire including demographic information, occupational anxiety, health anxiety, health cognition level were sent to 2281 students. The data were obtained using a convenience sampling. The distribution of the data is presented using median (minimum-maximum). Double-series correlation coefficient, chi-square test and polychoric correlation coefficient were used to evaluate the relationship between responses to the questionnaire. Results: A total of 885 students completed the questionnaire. The mean age was 21.47 (±2.62); 545 (61.9%) of them were female. A number of 809 (91.8%) students stated that they chose medical school willingly while 72 (8.2%) students unwillingly. Those who unwillingly chose medicine had a high desire to change their profession. The distance education was sufficient for 168 (19.2%) students; partially sufficient for 435 students. As the semester increased, their professional anxiety, awareness of the advantages and disadvantages of their professions, trust to the health system, ability of reviewing different professional resources increased. Female students were more concerned about their professional and general health than male students. Conclusions: Although the pandemic had effects on medical students' profession choice and anxiety levels, in general, these effects was not higher. The professional anxiety, awareness of the profession, trust to the health system, ability of reviewing different professional resources were related to semester year. Distance education systems should be improved. Psychological support can be provided for the students. Moreover the results should be evaluated regarding the time of study and expectations the end of pandemic in a short time that may be the reason more optimistic answers.
- Published
- 2020
17. How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance
- Author
-
Gholizadeh,Pourya, Köse,Åükran, Dao,Sounkalo, Ganbarov,Khudaverdi, Tanomand,Asghar, Dal,Tuba, Aghazadeh,Mohammad, Ghotaslou,Reza, Ahangarzadeh Rezaee,Mohammad, Yousefi,Bahman, and Samadi Kafil,Hossein
- Subjects
Infection and Drug Resistance - Abstract
Pourya Gholizadeh,1,2 Åükran Köse,3 Sounkalo Dao,4 Khudaverdi Ganbarov,5 Asghar Tanomand,6 Tuba Dal,7 Mohammad Aghazadeh,8 Reza Ghotaslou,8 Mohammad Ahangarzadeh Rezaee,8 Bahman Yousefi,8 Hossein Samadi Kafil2,9 1Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 2Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Department of Infectious Diseases and Clinical Microbiology, University of Health Sciences, Tepecik Training and Research Hospital, Ä°zmir, Turkey; 4Faculté de Médecine, de Pharmacie et d’Odonto-Stomatologie (FMPOS), University of Bamako, Bamako, Mali; 5Department of Microbiology, Baku State University, Baku, Republic of Azerbaijan; 6Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran; 7Department of Clinical Microbiology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey; 8Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 9Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranCorrespondence: Hossein Samadi KafilDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranTel +98 9127184735Fax +98 4133364661Email Kafilhs@tbzmed.ac.irAbstract: Rapid emergence of antibiotic-resistant bacteria has made it harder for us to combat infectious diseases and to develop new antibiotics. The clustered regularly interspaced short palindromic repeats –CRISPR-associated (CRISPR-Cas) system, as a bacterial adaptive immune system, is recognized as one of the new strategies for controlling antibiotic-resistant strains. The programmable Cas nuclease of this system used against bacterial genomic sequences could be lethal or could help reduce resistance of bacteria to antibiotics. Therefore, this study aims to review using the CRISPR-Cas system to promote sensitizing bacteria to antibiotics. We envision that CRISPR-Cas approaches may open novel ways for the development of smart antibiotics, which could eliminate multidrug-resistant (MDR) pathogens and differentiate between beneficial and pathogenic microorganisms. These systems can be exploited to quantitatively and selectively eliminate individual bacterial strains based on a sequence-specific manner, creating opportunities in the treatment of MDR infections, the study of microbial consortia, and the control of industrial fermentation.Keywords: antibiotic-resistant bacteria, CRISPR-Cas system, sequence-specific manner, resensitization, genome editing
- Published
- 2020
18. Expression of the adeB gene and responsiveness to 1-(1-naphthylmethyl)-piperazine and phenylalanyl-arginyl-β-naphthylamide in clinical isolates of Acinetobacter baumannii
- Author
-
Dal, Tuba, Aksu, Burak, Pagès, Jean-Marie, and Over-Hasdemir, Ufuk
- Published
- 2013
- Full Text
- View/download PDF
19. Oral Mikrobiyota Araştırmaları Işığında AğızSağlığına Yeni Bakış Açısı: Derleme
- Author
-
PAMUKÇU, UMUT, PEKER, İLKAY, YILDIZ, FATMA NUR, and DAL, TUBA
- Published
- 2018
20. Occult hepatitis B infection in Turkish HIV-infected patients: A multicentre, retrospective, cross-sectional study, Schindler study.
- Author
-
Ünlü, Gülten, Yıldız, Yeşim, Ören, Meryem Merve, Çabalak, Mehmet, Mete, Özlem, Kömür, Süheyla, Yıldırım, Figen, İnan, Dilara, Altunışık Toplu, Sibel, Akhan, Sıla, Karaşahin, Ömer, Akdemir Kalkan, İrem, Demir, Yakup, Ayhan, Merve, Karaoğlan, İlkay, Taşova, Yeşim, Bayındır, Yaşar, Dal, Tuba, and Çelen, Mustafa Kemal
- Subjects
HIV infection epidemiology ,HIV infection complications ,HIV infections ,HEPATITIS B ,RESEARCH ,DNA ,CROSS-sectional method ,RESEARCH methodology ,RETROSPECTIVE studies ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,DISEASE prevalence - Abstract
Objective: Occult hepatitis B infection (OHBI) appears to have a higher prevalence in populations at high risk for hepatitis B virus (HBV) infection with concomitant liver disease. The aim was to assess the prevalence of OHBI in a sample of human immunodeficiency virus -1 positive and HBV surface antigen-negative (HIV-1+/HBsAg-) Turkish patients.Methods: Ten centres in Turkey were included in the study. Patients were selected on the basis of a power calculation with a known population size of HIV-positive patients and a reported prevalence of OHBI. Gender, age, occupation, place of residence, treatment and clinical status, and laboratory results, including immunodeficiency panel, antibody tests, hemogram, biochemistry, and coagulation studies were evaluated retrospectively.Results: The number of HIV-infected patients followed in these centres was 3172 and the sample population numbered 278. All 278 were HBsAg negative. The mean age of the sample was 37.2 ± 13.1 years and 235 (84.5%) were male. All but one patient (99.6%) had been treated with antiretroviral therapy. Of the 278 patients, 169 (60.6%) were positive for Anti-HBs and 125 (44.8%) were positive for Anti-HBc IgG. HIV RNA was detected in 203/278 (73%) of the patients. Four HBV DNA (1.4%) were diagnosed with OHBI. There was no significant difference in hemogram, hemoglobin or bilirubin concentrations in those with OHBI compared with the other patients.Conclusion: In a representative sample of HIV+ patients from 10 Turkish centres, the prevalence of OHBI was found to be 1.4%. In HIV positive patients, it is important to identify those with OHBI for optimal clinical management and prognosis. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
21. Viral Hepatitis: Chronic Hepatitis B
- Author
-
DAL, TUBA and KÖSE, ŞÜKRAN
- Published
- 2018
22. Convalescent plasma therapy in patients with COVID‐19.
- Author
-
Yiğenoğlu, Tuğçe Nur, Hacıbekiroğlu, Tuba, Berber, İlhami, Dal, Mehmet Sinan, Baştürk, Abdülkadir, Namdaroğlu, Sinem, Korkmaz, Serdal, Ulas, Turgay, Dal, Tuba, Erkurt, Mehmet Ali, Turgut, Burhan, and Altuntaş, Fevzi
- Subjects
COVID-19 ,CLINICAL trials ,HEMAPHERESIS ,BLOOD banks - Abstract
There are currently no licensed vaccines or therapeutics for COVID‐19. Anti‐SARS CoV‐2 antibody‐containing plasmas, obtained from the recovered individuals who had confirmed COVID‐19, have been started to be collected using apheresis devices and stored in blood banks in some countries in order to administer to the patients with COVID‐19 for reducing the need of intensive care and the mortality rates. Therefore, in this review, we aim to point out some important issues related to convalescent plasma (CP) and its use in COVID‐19. CP may be an adjunctive treatment option to the anti‐viral therapy. The protective effect of CP may continue for weeks and months. After the assessment of the donor, 200‐600 mL plasma can be collected with apheresis devices. The donation interval may vary between countries. Even though limited published studies are not prospective or randomized, until the development of vaccines or therapeutics, CP seems to be a safe and probably effective treatment for critically ill patients with COVID‐19. It could also be used for prophylactic purposes but the safety and effectiveness of this approach should be tested in randomized prospective clinical trials. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
23. Antimicrobial Activity of Garlic Derivatives on Common Causative Microorganisms of the External Ear Canal and Chronic Middle Ear Infections.
- Author
-
Uzun, Lokman, Dal, Tuba, Kalcıoğlu, M. Tayyar, Yürek, Merve, Açıkgöz, Ziya Cibali, and Durmaz, Rıza
- Subjects
- *
EAR canal , *CANDIDA , *MIDDLE ear , *EAR infections , *ENTEROCOCCUS faecium , *ACINETOBACTER baumannii - Abstract
Objective: Today, antibiotic resistance is increasing and evolving into an important health problem. Therefore, it is important to research on alternative therapies to antibiotics. This study aimed to investigate the inhibitory effect of four garlic derivatives on microorganisms commonly isolated in ear infections. Methods: The antimicrobial activities of allicin, s-allyl cysteine (SAC), diallyl disulfide (DADS), and s-allyl mercaptocysteine (SAMC) were investigated on standard strains of commonly isolated microorganisms using the broth microdilution method. The test strains were selected among the microorganisms responsible for chronic suppurative otitis media and otitis externa. These microorganisms were Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecium, Candida albicans, and Candida tropicalis. Results: Minimum inhibitory concentration (MIC) values of allicin and SAC ranged from 0.125 to 20 µg/mL for fermentative bacteria (E. coli and K. pneumoniae), 20 to 80 µg/mL for non-fermentative bacteria (P. aeruginosa and A. baumannii), 5 to 10 µg/mL for gram-positive cocci (S. aureus and E. faecium), and 40 to 80 µg/mL for yeasts (C. albicans and C. tropicalis). MIC values of DADS ranged from 40 to 80 µg/mL for fermentative bacteria, 40 to 160 µg/mL for non-fermentative bacteria, 40 to 80 µg/mL for gram-positive cocci, and 20 to 40 µg/mL for yeasts. The MICs of SAMC were >640 µg/mL for the tested bacteria and yeasts. Conclusion: Both allicin and SAC showed antimicrobial activity against the tested microorganisms, even at low concentrations. These two derivatives may be used to treat infections in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
24. HBSAG, ANTI-HCV, ANTI-HIV 1/2, AND SYPHILIS SEROPREVALENCE IN BLOOD DONORS IN EASTERN MARMARA REGION, TURKEY AND AN OVERVIEW OF TRANSFUSION TRANSMITTED INFECTIONS IN TURKEY
- Author
-
Altındiş, Mustafa, Köroğlu, Mehmet, Mutlu, Birsen, Demiray, Tayfur, Dal, Tuba, Şahin, İdris, and Özçelik, Ümit
- Subjects
HBsAg ,Transfusion ,anti-HCV ,syphilis ,blood donors ,anti-HIV - Abstract
Altindis, Mustafa/0000-0003-0411-9669; WOS: 000375338000011 Introduction: Blood transfusion and component therapies are life-saving medical practices. However these therapies, are not without risks and may lead to the transmission of infectious agents from donor to recipient. Screening of blood donations for infectious agents is necessary to achieve transfusion safety. The aim of the study was to determine the six-years seroprevalence of HBV (hepatitis B virus), HCV (hepatitis C virus), HIV (human immunodeficiency virus), and syphilis in blood donors and to present an overview of transfusion transmitted infections in Turkey. Material and methods: A total of 150,787 volunteer blood donors who admitted to blood centers located in Eastern Marmara Region, Turkey between January 2009 and October 2014 were included in this study. HBsAg (hepatitis B surface antigen), anti-HCV (hepatitis C virus antibody), and HIV-1/2 antibody were detected by using Chemiluminescent Microparticle Enzyme Immunoassay (CMIA) method.. Syphilis screening was performed by using VDRL (Venereal Disease Research Laboratory) test in the first three years and by CMIA-based treponemal test in the last three years. Results: Among the blood donors, 140,536 (93.2%) were male and 10,251 (6.8%) female. Out of all donors, 2,019 (1.33%) donors were positive for at least one screening test. The seroprevalence of HBV, HCV, HIV, and syphilis were 0.8 % (n=1340), 0.38% (n=578), 0,0025% (n=38) and 0,004 % (n=63), respectively. A significant decrease was observed in HBV seroprevalence by years (p
- Published
- 2016
25. Investigation of carbapenemase and mcr-1 genes in carbapenem-resistant Klebsiella pneumoniae isolates.
- Author
-
Arabacı, Çiğdem, Dal, Tuba, Başyiğit, Tuğcan, Genişel, Neslihan, and Durmaz, Rıza
- Subjects
- *
KLEBSIELLA , *KLEBSIELLA pneumoniae , *CO-trimoxazole , *INFECTION prevention , *COLISTIN , *CARBAPENEMASE , *CEFEPIME - Abstract
Introduction: Carbapenem-resistant Klebsiella pneumoniae are a major problem. We aimed to investigate carbapenemase-encoding genes and transferable mcr-1 genes among 57 carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates from hospitalized patients. Methodology: Antibiotic susceptibility tests were performed by Phoenix (BD). Results for ertapenem and colistin were confirmed by gradient diffusion and microdilution methods. Carbapenemase and mcr-1 genes were investigated by Polymerase Chain Reaction (PCR). Results: Thirty-two (56.14%) isolates were from intensive care units (ICU). Antibiotic resistance rates by Phoenix: 52.63% for amikacin; 73.69% trimethoprim sulfamethoxazole; 91.23% cefepime; 82.46% tigecycline; 59.65% colistin. Carbapenemases positivity: 82.45% (47) for blaOXA-48, 40.35% (23) blaOXA-55, 3.50% (2) blaOXA-51, 1.75% (1) blaOXA-23, 1.75% (1) blaOXA-24, 1.75% (1) blaIMP. blaOXA-58, blaKPC, blaNDM-1, and blaVIM were not detected. Twenty (35.08%) isolates had both blaOXA-48 and blaOXA-55. Three isolates were mcr- 1 (+) and blaOXA-48 (+). One mcr-1 (+) isolates was blaOXA-51 (+). One colistin sensitive isolate determined by Phoenix, was found colistin resistant by microdilution. Conclusion: OXA-48 and OXA-55 co-harboring isolates and mcr-1 gene (+) isolates were spreading. Automated colistin susceptibility results should be confirmed by microdilution method. Resistance mechanisms in Enterobacteriaceae should be determined and the isolates should be monitored by molecular epidemiological methods. Effective infection control measures will contribute to reduce risk of antibiotic resistant bacterial infections and dissemination of antibiotic resistance. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
26. Effects of Formaldehyde and Vimentin Expression in Rat Skin
- Author
-
Kalkanli, Sevgi, Kalkanli, Nevin, Deveci, Engin, Dal, Tuba, and Baran, Özlem
- Subjects
Vimentina ,Formaldehyde ,Vimentin ,Formaldehído ,Piel ,Skin - Abstract
The Wistar rats ( 9 weeks old, 180200 g body weight) used in these trials were divided into two groups of 16 animals each (Control group and Experimental group). 100x65x100 in the sizes of the experimental group were taken into a glass vase. During the time period of 8 weeks, 5 days a week with 8 hours the inhalation of 10 ppm formaldehyde was made. Skin was removed and placed in 10% formaline. Sections were stained with Hematoxylene-Eosine, Trichrome-Masson and observed under light microscope. In this study, histopathological and immunohistochemical techniques due to the impact of the changes in formaldehyde and examined the distribution of vimentin. En este ensayo se utilizaron ratas Wistar (9 semanas de edad, 180-200 g de peso corporal) que se dividieron en dos grupos de 16 animales cada uno (grupos control y experimental). Los animales del grupo experimental fueron colocados en un vaso de vidrio de tamaño 100x65x100. Durante un período de tiempo de 8 semanas, 5 días a la semana con 8 horas se expuso a la inhalación de 10 ppm de formaldehído. Se retiró la piel y se colocó en formalina al 10%. Las secciones fueron teñidas con Hematoxilina-Eosina y Tricrómico de Masson, y fueron observadas al microscopio óptico. Las técnicas histopatológicas e inmunohistoquímicas y el impacto en la piel provocado por el formaldehído permitieron examinar la expresión de vimentina.
- Published
- 2013
27. Assessment of the underlying causes of the immune thrombocytopenia: Ten years experience.
- Author
-
Dal, Mehmet Sinan, Karakus, Abdullah, Dal, Tuba, Aydin, Berrin Balik, Hattapoglu, Elif, Ekmen, Mehmet Önder, Ulas, Turgay, and Ayyildiz, Orhan
- Published
- 2017
28. Evaluation of febrile neutropenic patients hospitalized in a hematology clinic.
- Author
-
Görük, Mücahit, Dal, Mehmet Sinan, Dal, Tuba, Karakus, Abdullah, Tekin, Recep, Özcan, Nida, and Ayyildiz, Orhan
- Subjects
FEBRILE neutropenia ,HEMATOLOGY ,HODGKIN'S disease ,LYMPHOBLASTIC leukemia ,CHRONIC myeloid leukemia - Abstract
Objective To evaluate the febrile neutropenic patients with hematological malignancies hospitalized in hematology clinic with poor hygiene standards. Methods A total of 124 patients with hematological malignancies (69 male, 55 female) hospitalized in hematology clinic with poor hygiene conditions depending on hospital conditions, between January 2007 and December 2010, were evaluated, retrospectively. Results In this study, 250 febrile neutropenia episodes developing in 124 hospitalized patients were evaluated. Of the patients, 69 were men (56%) and 55 women (44%). A total of 40 patients (32%) had acute myeloid leukemia, 25 (20%) acute lymphoblastic leukemia, 19 (15%) non-Hodgkin's lymphoma, 10 (8%) multiple myeloma, and 8 (8%) chronic myeloid leukemia. In our study, 56 patients (22%) were diagnosed as pneumonia, 38 (15%) invasive aspergillosis, 38 (15%) sepsis, 16 (6%) typhlitis, 9 (4%) mucormycosis, and 4 (2%) urinary tract infection. Gram-positive cocci were isolated from 52% ( n = 20), while Gram-negative bacilli 42% ( n = 16) and yeasts from 6% ( n = 2) of the sepsis patients, respectively. The most frequently isolated Gram-positive bacteria were methicillin-resistant coagulase-negative staphylococci ( n = 18), while the most frequently isolated Gram-negative bacteria was Escherichia coli ( n = 10). Conclusions Febrile neutropenia is still a problem in patients with hematological malignancies. The documentation of the flora and detection of causative agents of infections in each unit would help to decide appropriate empirical therapy. Infection control procedures should be applied for preventing infections and transmissions. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
29. Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey.
- Author
-
Çelen, Mustafa Kemal, Sayan, Murat, Dal, Tuba, Ayaz, Celal, Tekin, Alicem, Özekinci, Tuncer, Koruk, Suda Tekin, Barcin, Tunga, Tekin, Recep, Dal, Mehmet Sinan, and Kalkanlı, Sevgi
- Subjects
MOLECULAR epidemiology ,HIV-positive persons ,HIV infections ,PHYLOGENY ,ANTIRETROVIRAL agents - Abstract
Objective To detect the subtype characterization and drug-resistant mutations in HIV-1 strains after the refugee movement from Syria to Turkey between 2011 and 2014 in south east border lines. Methods A total of 65 patients were included in this study, of which 57 (88%) patients were antiretroviral therapy-naive patients. HIV-1 RNA was detected and quantified by real-time PCR assay. HIV-1 subtypes and circulating recombinant forms (CRFs) were identified by phylogenetic analysis (neighbor-joining method), and drug-resistant mutations were analyzed. Results Three major HIV groups were indicated. Two of these groups were located in subtype B. The other group showed heterogeneity. Subtype B (48/65, 73.8%), followed by CRFs (12/65, 18.5%) was the most common strain. Subtype of CRFs consisted of CRF01_AE (9/65, 13.8%) and CRF02_AG (3/65, 4.6%). Subtype C (1/65, 1.5%), sub-subtypes A1 (2/65, 3.1%) and F1 (2/65, 3.1%) were also detected with low prevalence. The rate of overall primary antiretroviral resistance was 4.9% (3/61). Drug-resistant rate for non-nucleoside reverse transcriptase inhibitors was 4.9%. The thymidine analogue mutation rate was 13.1% (8/61). Conclusions HIV molecular epidemiology studies are necessary to determine transmission patterns and spread. Subtype B and CRF01_AE, CRF02_AG are the most prevalent strains in the south-east of Turkey. However, subtype C, sub-subtypes A1 and F1 are of low prevalence but persist in the south-east of Turkey. In the near future, changing of HIV epidemiology will be possible in Turkey due to migration movement in border lines and resistance testing will play an important role in HIV management. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
30. Tenofovir disoproxil fumarate treatment in HbeAg-positive patients.
- Author
-
Ayaz, Celal, Çelen, Mustafa Kemal, Dal, Tuba, Deveci, Özcan, Bayan, Kadim, Mert, Duygu, Oruç, Ebru, Özcan, Nida, Kandemir, Idris, and Dal, Mehmet Sinan
- Published
- 2015
31. Is Chryseobacterium indologenes a shunt-lover bacterium? A case report and review of the literature.
- Author
-
Ozcan, Nida, Dal, Tuba, Tekin, Alicem, Kelekci, Selvi, Can, Sukran, Ezin, Ozgur, Kandemir, Idris, and Gul, Kadri
- Published
- 2013
32. A Rare Case of Mucormycosis in the Scalp.
- Author
-
HARMAN, Mehmet, UÇMAK, Derya, and DAL, Tuba
- Published
- 2013
- Full Text
- View/download PDF
33. Orthophenylphenol in healthcare environments: a trial related to a new administration method and a review of the literature.
- Author
-
TEKİN, Alicem, DAL, Tuba, SELÇUK, Caferi Tayyar, DEVECİ, Özcan, TEKİN, Recep, METE, Mahmut, DAYAN, Saim, and HOŞOĞLU, Salih
- Subjects
- *
PHENYLPHENOLS , *MEDICAL care , *ANTIBACTERIAL agents , *ANTIFUNGAL agents , *DRUG efficacy , *LITERATURE reviews - Abstract
Aim: To investigate antibacterial and antifungal effectiveness of orthophenylphenol (Fumispore, LCB, La Salle, France) with a new administration method in healthcare environments. Materials and methods: This study was performed in 3 units of the Dicle University Hospital. Surface swab and ambient air samples were collected just before the application of Fumispore and 6 h later. All samples were inoculated onto plate count agar and Sabouraud dextrose agar. The total aerobic colony count (ACC) was determined by using the standard method based on quantitative bacterial measurement. Results: In total, 30 surface swabs and 6 ambient air samples were collected. The mean values of the total ACC were determined on surface swabs: 12.1 colony CFU/cm² for total microorganisms and 2.9 CFU/cm² for fungi before application, and 1.6 CFU/cm² for total microorganisms and 0.4 CFU/cm² for fungi after application. The mean values of the total ACC were detected in ambient air samples: 258 CFU/m³ for total microorganisms and 208 CFU/m³ for fungi before application, and 20 CFU/m³ for total microorganisms and 15 CFU/m³ for fungi after application. Conclusion: The total ACC values obtained before and after applications have shown that orthophenylphenol (Fumispore) is suitable for adequate disinfection in healthcare environments. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
34. Evaluation of cutaneous palpebral anthrax.
- Author
-
Tekin, Recep, Ari, Şeyhmus, Dal, Tuba, Kaya, Şafak, Kortak, Mehmet Zeki, Dursun, Birgül, and Dayan, Saim
- Subjects
ANTHRAX diagnosis ,RARE diseases ,BACILLUS anthracis ,ANTHRAX treatment ,ZOONOSES ,ANIMAL culture ,HOSPITAL admission & discharge - Abstract
Context: Anthrax is a rare disease caused by Bacillus anthracis. Antrax is zoonotic disease and is often encountered in persons engaged in animal husbandry. Cutaneous anthrax is approximately 95% of anthrax in humans. Palbebral involvement is rare. Objective: In this study, we aimed to evaluate the clinical presentation, diagnosis and treatment of cases with cutaneous palpebral anthrax. Methods: In this study, the patients diagnosed of cutaneous palpebral anthrax between January 2000 and December 2012, were investigated and evaluated, retrospectively. Cutaneous palpebral anthrax was diagnosed by the presence of typical anthrax lesion and/or observation of gram-positive encapsulated bacilli in gram prepations and/or culture positive of samples taken from lesions. In the cases who were culture-negative and without bacilli in gram-staining, the diagnosis was based on the presence of characteristic clinical presentation with a history of severe scarring formation, swelling, black eschar and positive response to the treatment. Results: A total of 21 patients with cutaneous palpebral anthrax admitted to the two hospitals between January 2000 and December 2012. Eight patients were male (38.1%) and 13 patients were female (61.9%), and the mean age was 31 ± 21.2 (range 1-82 years). The most common symptoms on admission to the hospital were swelling and redness on the skin. Periorbital lesions were in the right eye in 14 cases and the most common eyelid involvement was seen in upper eyelid with 15 cases. The diagnosis was based on isolation of bacteria in five (23.8%) cases, detection of gram-positive bacilli in direct examination of characteristic lesion material in six (28.5%) cases. Ten (47.7%) cases were diagnosed by the characteristic appearance of the lesion. Malignant pustule was seen in all of our patients and seven cases (33.4%) had malignant edema. In the treatment, penicilin was used for 10 (47.7%) cases, ampicillin-sulbactam for five (23.8%) cases and, ciprofloxacin for three (14.3%) cases. Cicatricial ectropion was observed in 10 (47.7%) patients, lagophthalmos developed in four (19%) patients, and corneal scar in two (9.5%) patients. The distribution of the cases did not differ by the year but showed a density in the months from July to September (62.7%). Conclusion: Early diagnosis and high dose antibiotic treatment can facilitate the treatment and prevent development of eyelid complications including cicatricial ectropion, corneal scars and palpebral symphysis. Prolonged follow-up is necessary in patients who develop complications and surgical intervention. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
35. Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey.
- Author
-
ÇİÇek, Ayşegül Çopur, Düzgün, Azer Özad, Saral, Ayşegül, Kayman, Tuba, Çİzmecİ, Zeynep, Balcı, Pervin Özlem, Dal, Tuba, Fırat, Mehmet, Tosun, İsmail, Alıtntop, Yasemin Ay, Çalışkan, Ahmet, Yazıcı, Yelda, and Sandallı, Cemal
- Subjects
INTEGRONS ,ACINETOBACTER baumannii ,POLYMERASE chain reaction ,ANTI-infective agents ,DISEASE susceptibility ,SCIENTIFIC observation ,CEFEPIME - Abstract
Abstract: Objective: To investigate the antibiotic resistance genes inserted into class 1 and class 2 integrons in Acinetobacter baumannii (A. baumannii) isolates obtained from nine different cities in Turkey. Methods: A collection of 281 A. baumannii clinical isolates were collected from nine diferent state hospitals in Turkey and were confirmed as A. baumannii by conventional biochemical, API testing and bla
-OXA-51 specific PCR. The isolates were examined by PCR for existence of class 1 and 2 integron gene cassettes. Results: They were characterized by antimicrobial susceptibility testing and the highest resistance rates were determined for piperacillin (90.03%), ciprofloxacin (87.54%), cefepime and trimethoprim/sulfamethoxazole (81.13%). The lowest resistance rates was for cefotaxime (3.55%). class I integrons were detected in 6.4% (18/281) of A. baumannii strains and no class 2 integron was detected. The gene cassettes of class 1 integrons AacC1-AAC(3) I-aadA1, AacC1-aadA1, AAC(3)-I, AAC(3)-I -AAC(3)-I -aadA1, TEM-1, AAC(3)-I-aadA1 – AAC(3)-I -AAC(3)-I, AAC(3)-I -AAC(3)-I -AAC(3)-I -aadA1, AAC(3)-I – aadA1, AAC(3)-I-AAC(3)-I, AAC(3)-I-aadA1-AAC(3)-I-aadA1, AAC(3)-I-AAC(3)-I-aadA1-AAC(3)-I-aadA1 were detected in eighteen strains. The aac genes family were most frequently found integrated into the class 1 integrons and it was followed by aadA genes and TEM-1 genes. Conclusions: This is an extensive study on the distribution of class 1 integron among A. baumannii in Turkey. In addition to these, two new alleles were observed. Their percentage rates of similarity to other cassettes are 95% aadA1 (TKA18) and 89% aadA1 (ANKA3). [Copyright &y& Elsevier]- Published
- 2013
- Full Text
- View/download PDF
36. Carbapenem resistance in Acinetobacter baumannii: where is it heading?
- Author
-
Deveci, Özcam, Dal, Tuba, Tekin, Recep, Bozkurt, Fatma, Tekin, Alicem, and Dayan, Saim
- Published
- 2013
37. Assessment of methicillin and clindamycin resistance patterns in Staphylococcus aureus isolated from a tertiary hospital in Turkey.
- Author
-
Tekin, Alicem, Dal, Tuba, Deveci, Özcan, Tekin, Recep, Atmaca, Selahattin, and Dayan, Saim
- Published
- 2013
38. Soft Tissue Abscess Caused by Aspergillus Fumigatus in an Immunosuppressive Patient.
- Author
-
Dal, Tuba, Tekin, Alicem, Tekin, Recep, Deveci, Özcan, Fırat, Uğur, Mete, Mahmut, and Dayan, Saim
- Abstract
Aspergillosis is a definition including a wide variety of diseases caused by fungi in the genus Aspergillus. Aspergillosis that occurs especially in neutropenic and immunosuppressive patient is an opportunistic infection with a very high mortality rate. In this study, we presented a case of primary cutaneous aspergillosis that caused in a heart transplant recipient. Thirty-nine-years-old male patient with the complaints of high fever lasting for ten days, redness of the right thigh, swelling, and pain, was admitted to our hospital. By the tissue ultrasonography of right thigh, 3x12 cm in size of septated intensive fluid collection (abscess?) in muscle tissue at a depth of 1.5 cm to the skin surface was reported. The biopsy specimens that were taken from the lesion sent to microbiology and pathology laboratories. The branching septate hyphae were observed in Hematoxylin-Eosin and Gram stained preparations. Aspergillus fumigatus was isolated and identified from the biopsy specimen. The patient's symptoms were regressed rapidly and the lesion was improved by surgical debridement and antifungal agent (amphotericin B) therapy. In the light of this case report, we were emphasized that microbiologic examination and culture have an important role for diagnosis of cutaneous aspergillosis. [ABSTRACT FROM AUTHOR]
- Published
- 2013
39. Idiopathic thrombocytopenic purpura associated with splenic tuberculosis: case report.
- Author
-
Dal, Mehmet Sinan, Dal, Tuba, Tekin, Recep, Bodakçi, Erdal, Düzköprü, Yakup, and Ayyildiz, Mehmet Orhan
- Published
- 2013
40. Septic arthritis caused by Aspergillus fumigatus in an immunosuppressive patient: A case report and review of the literature.
- Author
-
Dal, Tuba, Tekin, Alicem, Deveci, Özcan, Bulut, Mehmet, Fırat, Uğur, and Mete, Mahmut
- Subjects
- *
INFECTIOUS arthritis , *ASPERGILLUS fumigatus , *ASPERGILLOSIS , *IMMUNOSUPPRESSION , *PEOPLE with diabetes - Abstract
Septic arthritis is a serious medical problem that causes rapidly chronic and irreversible joint damage when diagnosis and treatment are delayed. Although, the bacteria are the most frequent cause of septic arthritis, rarely fungi may be responsible for this disease. We presented here a case of septic arthritis caused by Aspergillus fumigatus, developed in a 65 years-old-male patient with diabetes mellitus. The patient admitted to our hospital with complaints of pain, swelling, redness in the right knee and high fever, which are not exceeding despite using vancomycin and ceftriaxone for 18 days. Imipenem was started to the patient in our hospital. At the end of 7 days because of no regression of patient's complaints, arthroscopic synovectomy and lavage were performed for the patient's right knee. Aspergillus fumigatus was isolated from the aspiration biopsy specimen that received during the surgical operation and patient was treated with voriconazole, successfully. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
41. Macrophage migration-inhibitory factor is elevated in pregnant women with gestational diabetes mellitus.
- Author
-
Yilmaz, Özgür, Küçük, Mert, Kebapçilar, Levent, Altindag, Tamer, Yüksel, Arif, Yuvanç, Hüseyin Oguz, Dal, Tuba, and Savran, Yusuf
- Subjects
MACROPHAGE migration inhibitory factor ,GESTATIONAL diabetes ,GLUCOSE ,HOMEOSTASIS ,CARBOHYDRATE intolerance ,SERUM - Abstract
Objective: In reports, abnormal macrophage migration-inhibitory factor (MIF) production has been associated with several diseases. Furthermore, despite scarce data, increasing evidence suggest that MIF plays a central role in glucose homeostasis and in the development of type 1 and type 2 diabetes. However, serum MIF levels in gestational diabetes mellitus (GDM) have not yet been investigated. To address this question, we performed a prospective study between a group of pregnant women with GDM and healthy pregnant controls. Materials and methods: GDM group consisted of 43 pregnant women, whereas the control group consisted of 40 healthy pregnant women. In the morning after an overnight fast, venous blood was sampled for the measurement of serum concentrations of insulin and MIF. Serum was separated by centrifugation and immediately stored at −80°C until the assay. Results: There was no significant difference between the groups for maternal characteristics. Women with GDM had significantly higher levels of serum insulin (14.37 ± 9.92 µU/ml vs. 8.78 ± 4.35 µU/ml; p == 0.001) and serum MIF concentrations (11.31 ± 4.92 ng/ml vs. 5.31 ± 4.07 ng/ml; p < 0.001) when compared with healthy pregnant control group. Conclusion: Our data demonstrated that serum levels of MIF are significantly elevated in patients with GDM. Our findings indicate that MIF might have a role in GDM; however, there is a need for further investigation. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
42. Expression of the adeB gene and responsiveness to 1-(1-naphthylmethyl)-piperazine and phenylalanyl-arginyl-[beta]-naphthylamide in clinical isolates of Acinetobacter baumannii.
- Author
-
Dal, Tuba, Aksu, Burak, Pagès, Jean-Marie, and Over-Hasdemir, Ufuk
- Published
- 2013
- Full Text
- View/download PDF
43. Urogenital myiasis caused by Psychoda albipennis.
- Author
-
Çiçek, Mutalip, Diker, Ali Ihasan, Ipek, Duygu Neval Sayin, Tekin, Alicem, and Dal, Tuba
- Published
- 2012
- Full Text
- View/download PDF
44. Klebsiella Pneumoniae Kan İzolatlarında Karbapenem ve Kolistin Direnç Mekanizmalarının Moleküler Olarak İncelenmesi
- Author
-
GENİŞEL, Neslihan, ATMACA, Selahattin, ÖZCAN, Nida, GÜL, Kadri, AKPOLAT, Nezahat, DAL, Tuba, KENAR, Levent, and ALTANLAR, Nurten
- Subjects
Klebsiella pneumoniae,carbapenemase,bla OXA-48,mcr-1,multiplex PCR,broth microdilution ,Klebsiella pneumoniae,karbapenemaz,bla OXA-48,mcr-1,multipleks PZR,sıvı mikrodilüsyon - Abstract
Karbapenem Dirençli Klebsiella pneumoniae KDKp enfeksiyonları azalan tedavi seçenekleri nedeniyle endişe verici sağlık sorunları oluşturmaktadır. Bu çalışmada, KDKp izolatlarının karbapenemaz OXA-23,24, 48, 51, 55, 58, KPC, NDM-1, VIM, IMP ve mcr-1 genleri araştırılmaktadır. Dicle Üniversitesi Tıp Fakültesi Hastanesi yoğun bakım ünitelerinden YBÜ Şubat 2020 ile Haziran 2020 tarihleri arasında alınan hasta kan örneklerinden izole edilen toplam 33 KDKp izolatı çalışmaya dahil edildi. Tüm KDKp izolatları, bla OXA-23, 24, 48, 58, bla KPC, blaNDM-1, bla VIM, bla IMP dahil olmak üzere karbapenemaz kodlayan genlerin varlığı multipleks Polimeraz Zincir Reaksiyonu PZR ile araştırıldı. KDKp izolatları monoplex PZR ile mcr1 geni ve bla OXA-51, bla OXA-55 genleri için test edildi. Kirby Bauer Disk Difüzyon Test DDT %100 ile çalışılan tüm KDKp izolatları ertapenem’e dirençliydi; 9’u %2.27 imipeneme, 23’ü %69.70 meropeneme dirençliydi. İzolatların 20’si %60,61 kolistine dirençli bulundu. bla OXA-48, bla NDM-1 ve bla OXA-24 genleri sırasıyla %75.76 n=25 , %6.06 n=2 ve %3.03 n=1 izolatında bulundu. İki %6.06 izolatta hem bla OXA-48 hem de bla NDM-1 genleri, 16 %48,48 izolatta mcr-1 geni saptandı. Kolistin minimum inhibitör konsantrasyonu MİK değeri 2 µg/ml olan 13 hastada ortalama yatış süresi 20.3 gün iken, kolistin MİK değeri > 2 µg/ml olan 20 hastada 33.9 gündü. Hastanede ortalama kalış süresi mcr-1 negatif hastalarda 21.8 gün, mcr-1 pozitif hastalarda 35.7 gündü. Karbapenemaz ve mcr1 pozitiflikleri Diyarbakır, Türkiye’de çarpıcı biçimde yüksek oranlarda bulundu. Kp izolatlarında plazmit aracılı antimikrobiyal direncin sorunlu olduğu belirtildi. Her hastane moleküler yöntemlerle kolistin ve karbapenem direnç mekanizmalarını izlemelidir. Kolistin direnci sıvı mikrodilüsyon yöntemi ile doğrulanmalıdır, Carbapenem-Resistant Klebsiella pneumoniae CRKp infections are worrying health problems due to decreasing treatment options. This study investigates the carbapenemase OXA-23,24, 48, 51, 55, 58, KPC, NDM-1, VIM, IMP and mcr-1 genes of the CRKps isolates A total of 33 CRKp isolates isolated from patient blood samples from the Dicle University Medical Faculty Hospital, intensive care units ICUs between February 2020 and June 2020, were included in the study. The presence of carbapenemase encoding genes -including all CRKp isolates, bla OXA-23, 24, 48, 58, bla KPC, blaNDM-1, bla VIM, bla IMP- were investigated by multiplex Polymerase Chain Reaction PCR . CRKp isolates were tested for mcr-1 gene and bla OXA-51, bla OXA-55 genes by monoplex PCR. All CRKp isolates studied with Kirby Bauer Disc Diffusion Method DDM 100% were resistant to ertapenem, 9 27.27% resistant to imipenem, and 23 69.70% were resistant to meropenem. 20 60.61% of the isolates were found resistant to colistin. bla OXA-48, bla NDM-1 and bla OXA-24 genes were found in 75.76% n = 25 , 6.06% n = 2 and 3.03% n = 1 isolates, respectively. Both bla OXA-48 and bla NDM-1 genes were detected in two 6.06% isolates and mcr-1 gene in 16 48.48% isolates. While the mean hospitalization was 20.3 days in 13 patients with a colistin minimum inhibitory concentration MIC of 2 µg/ml, it was 33.9 days in 20 patients with a colistin MIC of > 2 µg/ml. The average length of stay in the hospital was 21.8 days in mcr-1 negative patients and 35.7 days in mcr-1 positive patients. Carbapenemase and mcr-1 positivities were found at dramatically high rates in Diyarbakır, Turkey. It was indicated that plasmid-mediated antimicrobial resistance in Kp isolates was problematic. Each hospital should monitor the colistin and carbapenem resistance mechanisms by molecular methods. Colistin resistance should be confirmed by the broth microdilution method BMD
45. Real-life Data for Tenofovir Alafenamide Fumarate Treatment of Hepatitis B: the Pythagoras Cohort.
- Author
-
Karasahin, Omer, Kalkan, Irem Akdemir, Dal, Tuba, Toplu, Sibel Altunısık, Harputoğlu, Murat, Mete, Ayşe Ozlem, Kömür, Süheyla, Sarigul, Figen, Yildiz, Yesim, Esmer, Fatih, Kandemir, Ozlem, Nazik, Selcuk, Inan, Dilara, Akgul, Fethiye, Kaya, Safak, Tunc, Nurettin, Balın, Safak Ozer, Bayındır, Yasar, Tasova, Yesim, and Akar, Fesih
- Subjects
- *
GLOMERULAR filtration rate , *BALDNESS , *TENOFOVIR , *RETROSPECTIVE studies , *TERTIARY care , *MANN Whitney U Test , *OSTEOPOROSIS , *CHI-squared test , *FRIEDMAN test (Statistics) , *DATA analysis , *BONE density , *CHRONIC hepatitis B , *LONGITUDINAL method - Abstract
Background: Chronic hepatitis B (CHB) is a viral infection that can result in life-threatening conditions, such as hepatocellular carcinoma and cirrhosis. Tenofovir, which is used for the treatment of CHB, is a nucleotide analog that inhibits HBV-DNApolymerase and has two formulations: disoproxil and alafenamide. In contrast to tenofovir disoproxil fumarate (TDF), tenofovir alafenamide fumarate (TAF) penetrates the whole hepatocyte without being eliminated due to its longer plasma half-life and greater plasma stability. As a result, side effects such as proximal renal tubulopathy and loss of bone density are less common in the treatment of TAF and have similar efficacy to TDF. Objectives: The purpose of the study was to evaluate the effectiveness and reliability of TAF using real-life data. Methods: This retrospective cohort study was carried out in secondary or tertiary healthcare centers in southern Turkey. A total of 480 patients aged 18 years and older were administered TAF for an appropriate indication by the infectious diseases and gastroenterology clinics of the healthcare centers participating in this study. The data collected at t = 0, t = 3, and t = 6 months of treatment were analyzed. The chi-square, Mann-Whitney U, Friedman, Wilcoxon, Cochran's Q, and McNemar's tests were used. Results: The mean age of the patients was 47.40 ± 14.5, and 327 of them (68.1%) were male. A total of 78.l% of the 480 patients who underwent the TAF treatment had previous antiviral therapy experience (TDF, n = 340; 70.8 %), and 2l.9% were treatment-naive. The most common reasons for the initiation of TAF treatment were the use of drugs affecting bone mineral density (BMD) (42.9%) and osteoporosis (22.3%). Patients who had taken TDF experienced a significant improvement in glomerular filtration rate (GFR), hip and spine T-scores, and phosphorus levels from t = 0 months to t = 6 months after switching to TAF (P < 0.05). For this group, no statistically significant difference was observed concerning LDL and cholesterol levels from t = 0 months to t = 6 months. Side effects were reported by 5.7% of patients in the third month and 7.l% in the sixth month, with the most common side effect being hair loss (l%). Conclusions: TAF was found to be an effective and safe alternative to TDF with lower incidences of its long-term effects, such as nephrotoxicity and decreased bone density. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
46. INVESTIGATION OF GROUP A, C, G BETA STREPTOCOCCI IN CHILDREN WHO ADMITTED TO THE HOSPITAL WITH A COMPLAINT OF SORE THROAT.
- Author
-
Tekeş, Hilal Zeynep, Yürek, Merve, Yürek, Burak, and Dal, Tuba
- Subjects
THROAT diseases ,PHARYNGITIS ,SEX discrimination ,STREPTOCOCCUS ,DNA probes ,PHARYNX - Abstract
Sore throat is constitutes 1-5% of all applications. Acute tonsillopharyngitis is the most common cause of pain in the throat. Viral agents of acute tonsillopharyngitis are EBV, CMV, Adenovirus, Rhinovirus .The most common bacterial cause of acute tonsillopharyngitis is group A Beta Hemolytic streptococci (GAS, Streptococcus pyogenes). Untreated Acute tonsillopharyngitis is common complaint in outpatient practice in pediatric practice may be non-suppurative as peritonsil abscess, otitis, mastoiditis after pharyngitis, or non-SUPURATIVE complications such as acute rheumatoid fever (ARA) and acute glomerulonephritis (AGN). Group A betahemolytic streptococci are separately according to M proteins. Of these types, 1,3,5,6,18,19,24 are more responsible for pyodermia and AGN and type 49,55,57 can cause rheumatic fever. Group C and G can cause pharyngitis and AGN and can not cause rheumatic fever. Group A beta hemolytic streptococcal infections are frequently seen in 5-15 years of age, regardless of gender discrimination. It can cause epidemics in crowded environments such as nests, schools and barracks by passing through people through droplets. It can also pass through food, water (human contamination), contaminated dust, clothes, blankets and other items. Group A beta hemolytic streptococcal pharyngitis is characterized by sudden sore throat, fever, headache, dysphagia, abdominal pain, nausea, vomiting, cervical LAP (lymphadenopathy). Diagnosis is made with clinical and throat culture. Tonsil or posterior pharyngeal culture is taken. The false negativity of cultures is around 10%.Carrier distinguish can not do with growing in the culture or not grow. There is also a rapid diagnostic test of streptococci with group A carbohydrate antigen acid extraction. The specificity and sensitivity of this test depends on the appropriate culture taking . Detection of DNA probes by optical immunoassay and combination is also rapid and novel. Throat culture in a child with pharyngitis should be made by considering the age, clinical signs and symptoms of the patient, season, family and community epidemiology, contact history, presence of ARA or AGN in the family. In addition, after treatment, individuals with symptoms should also be cultured. In group A beta hemolytic streptococcal pharyngitis, infectivity is high during acute infection. In untreated cases, transmission is reduced after the first week, and 24 hours after the appropriate antibiotic treatment, the transmission is lost. Despite the decrease in the number of bacteria in the carriers and the risk of transmission due to the removal of the bacteria by nasal secretions, these individuals may be the source of GAS in the community. Group C, G beta hemolytic streptococci can be found as asymptomatic colonized in pharynx. Some of these individuals may develop invasive disease. However, the treatment of Group C, G beta hemolytic streptococci isolated individuals is a controversial. [ABSTRACT FROM AUTHOR]
- Published
- 2018
47. INVESTIGATION OF THE ANTIMICROBIAL EFFECT OF TURKISH PINE EXTRACT.
- Author
-
ÇOBANOĞLU, Ahmet Selçuk, KÖKLÜ, Ramazan, and DAL, Tuba
- Subjects
ESCHERICHIA coli ,TURBIDITY ,PLANT extracts ,KLEBSIELLA pneumoniae ,CANDIDA albicans ,ANTI-infective agents ,ANTIBACTERIAL agents - Abstract
Background and aim: Turkish pine extract, a type of oil, is a half liquid substance with a vigorous odor. The Turkish pine extract "Pinus brutia" is a prevalent type in Turkey. It is traditionally used topically by humans and animals in Anatolia. Investigating the antimicrobial effect of the Pinus brutia extract was the purpose of this research. Material and methods: In this study, antimicrobial effect of Turkish pine extract in Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae RSKK 574, Candida albicans ATCC 10231 strains were investigated by agar diffusion. Strains were inoculated in a sterile tube containing saline and the turbidity was set do 1,5x108 μg/ml. Bacterial suspensions were spread on the surface of the Muller-Hinton medium. Wells with a diameter of 6mm were cut open in the medium by a bisturi. We added 50 μl of Turkish pine extract inside the wells and (36 oC) incubated for 24 hours (48 hours for C.albicans) then inhibition zones were calculated in terms of mm's. Ciprofloxacin and fluconazole (C.albicans) were used as control. Results: Inhibition zones of S.aureus and P.aeruginosa were detected as 13mm and 8mm, respectively. Inhibition zone of medium inoculated C.albicans was 16mm. No zones of inhibition were seen for K.pneumonia and E.coli Discussion: Only a few number of studies were aimed to investigate the antimicrobial effect of Turkish pine extract. It was indicated that "Pinus brutia" was effective on P.aeruginosa, S.aereus, B.subtilis, E.coli and C.albicans. In our study, it was demonstrated that Turkish pine extract was effective on S.aureus, P.aeruginosa, and C.albicans. The esensial oil compounds, species diversity, lack of standardization in the procedure, could have an impact on the results. Evaluation of the minimum inhibitor concentration of the Turkish pine extract by microdilution and cytotoxicity tests are required. After determining its effectiviness and reliability, Turkish pine extract can be used as a topical antimicrobial. [ABSTRACT FROM AUTHOR]
- Published
- 2018
48. CARBAPENEM RESISTANT ENTEROBACTERİCEA STRAINS CAN KLORAMPHENICOL BE AN ALTERNATIVE?
- Author
-
EMİR, Mehmet Salih, YAVAŞ, Ahmet Murat, and DAL, Tuba
- Subjects
CARBAPENEMS ,ANTIBIOTICS ,DRUG resistance ,GRAM-negative bacteria ,INFECTION ,DISC diffusion tests (Microbiology) - Abstract
Introduction: In recent years, antibiotic resistance in gram negative bacteria has become an important problem. An important cause of mortality in hospitalized patients is infections caused by members of the Enterobacteriacea family, who have multiple antibiotic resistances. Previously, beta lactam group antibiotics were widely used in the treatment of these infections. Carbapenem has been used in the treatment of gram negative bacterial infections with the spread of extended-spectrum betalactamase and AmpC beta lactamase producing strains. However, nowadays, the frequency of infections due to isolates that a reresistant to carbapenems by increasing the production of carbapenemase is increasing. In infections caused by carbapenem resistant gram negative bacteria, resistance to colistin, the lastoption antibiotic, can be spreadhorizontally in the community and hospital environment with the plasmid mcr- 1 gene. All of these reasons, different antibiotic resistance isolates for different treatment options have brought to the agenda. In this study, we aimed to investigate the chloramphenicol susceptibility in carbapenem resistant Klebsiellapneumoniae isolates. Material and Methods: In this study 31 carbapenem resistant Klebsiellapneumoniae isolates isolated from clinical samples of two hospitalized hospitalized patients were included in this study. Chloramphenicol sensitivity was investigated by disk diffusion method in isolates. For this, 0.5 McFarland bacteria solutions were spread on the surface of Müller-Hinton agar media; chloramphenicol discs (30 μg / ml) were placed; After incubation at 37 ° C, the results were evaluated according to the recommendations of the Clinical and Laboratory Standards Institute. Results: In the study, 31 of 31 Klebsiellapneumoniae isolates were susceptible to chloramphenicol (45.1%) and 2 (6.4%) were susceptible. Discussion: In patients with multiple antibiotic resistance Klebsiellapneumoniae infections, chloramphenicol may be an alternative treatment option. However, side effects such as bone marrow suppression, aplastic anemia, neurotoxicity, optic neuritis and gray infant syndrome should be kept in mind. [ABSTRACT FROM AUTHOR]
- Published
- 2018
49. Methicillin-Resistant Staphylococcus Strains Isolated from Adult Intensive Care Units with E-test MIC Values of Different Antibiotic Research.
- Author
-
Gündoğuş, Narin, Gül, Kadri, Dal, Tuba, and Akpolat, Nezahat
- Subjects
- *
METHICILLIN-resistant staphylococcus aureus , *INTENSIVE care units , *ANTIBIOTICS - Abstract
Objective: Nasocomial infections are major health problems due to their high morbidity and mortality, prolonged hospital duration and higher treatment costs. Methicillin-resistant staphylococcus species became one of the leading bacteria causing nasocomial infections especially in intensive care units, recently. The minimum inhibitory concentration value of an antibiotic gives the concentration of antibiotic needed to inhibit the bacteria in the infection area. Careful monitoring of minimal inhibitory concentration (MIC) values is necessary especially during long-term treatments of meticillin-resistant Staphylococcus aureus (MRSA) and meticillin-resistant coagulase-negative staphylococci (MRCoNS) infections1,2. Increasing antibiotic resistance in methicillin-resistant staphylococci, has led to the need for different antibiotics. Methods: A total of 60 meticillin-resistant staphylococci strains isolated in Microbiology Laboratory of Dicle University Hospital, from clinical specimens of patients in adult İntensive Care Units (ICUs) between April 2013 and March 2014 were included in this study. After identification with conventional and automated system, the antibiotic susceptibility rates of vancomycin, teicoplanin, daptomycin, linezolid, quinupristin/dalfopristin, tigecycline, ceftaroline were determined by E-test method. Results: The majority of the samples (26.7%) were sent from Pulmonary Diseases and Tuberculosis intensive care unit and the blood samples were the most common materials (80%). All staphylococcal strains in our study were determined as susceptible to vancomycin, daptomycin, linezolid, teicoplanin and tigecycline. One (1.6%) MRCoNS isolate was resistant to quinupristin/dalfopristin while 11 (36.6%) of the MRSA isolates were resistant to ceftaroline. In comparison with the MIC values of MRSA and MRCoNS, only tigecycline was significantly different. Thirty MRSA strains were evaluated in terms of vancomycin-intermediate Staphylococcus aureus/heteroresistant vancomycinintermediate Staphylococcus aureus (VISA/hVISA) with macro E-test method; any VISA/hVISA isolate was not detected. Antibiotic concentrations below the MIC level, not only leads to treatment failure but also causes mutant bacteria to appear. In order to control the resistance to antibiotics in the treatment of infections due to MRSA and MRCoNS agents, the clinician should be notified of the MIC values of the drugs and the treatment should be planned accordingly. VISA/hVISA isolates should be considered in treatment failures of infections due to MRSA which are in vitro susceptible to vancomycin. Further testing is needed to detect these isolates. Despite the fact that ceftaroline is not a drug used in our country, the high resistance rate in our study is remarkable. This situation may be due to the intensive use of other beta-lactam antibiotics. Therefore, antibiotic susceptibility results should be taken into consideration during planning the treatment of infections. The high average MIC values of tigecycline in MRCoNS infections should also be monitored carefully. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
50. Risk factors for nosocomial burn wound infection caused by multidrug resistant Acinetobacter baumannii.
- Author
-
Tekin, Recep, Dal, Tuba, Bozkurt, Fatma, Deveci, Ozcan, Palanc, Ylmaz, Arslan, Eyüp, Selçuk, Caferi Tayyar, Hosoglu, Salih, and Hoşoğlu, Salih
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.