50 results on '"Choi, Man Soo"'
Search Results
2. Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo
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Kwon, Eun-Bin, Kim, Young Soo, Hwang, Youn-Hwan, Kim, Buyun, Lee, Sang-Beom, Park, Soo Kwon, Choi, Man Soo, Ha, Hyunil, and Choi, Jang-Gi
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- 2022
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3. The patterns of deleterious mutations during the domestication of soybean
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Kim, Myung-Shin, Lozano, Roberto, Kim, Ji Hong, Bae, Dong Nyuk, Kim, Sang-Tae, Park, Jung-Ho, Choi, Man Soo, Kim, Jaehyun, Ok, Hyun-Choong, Park, Soo-Kwon, Gore, Michael A., Moon, Jung-Kyung, and Jeong, Soon-Chun
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- 2021
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4. Rice CaM-binding transcription factor (OsCBT) mediates defense signaling via transcriptional reprogramming
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Chung, Jung-Sung, Koo, Sung Cheol, Jin, Byung Jun, Baek, Dongwon, Yeom, Seon-In, Chun, Hyun Jin, Choi, Man Soo, Cho, Hyun Min, Lee, Su Hyeon, Jung, Wook-Hun, Choi, Cheol Woo, Chandran, Anil Kumar Nalini, Shim, Sang In, Chung, Jong-Il, Jung, Ki-Hong, and Kim, Min Chul
- Published
- 2020
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5. Genetic diversity patterns and domestication origin of soybean
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Jeong, Soon-Chun, Moon, Jung-Kyung, Park, Soo-Kwon, Kim, Myung-Shin, Lee, Kwanghee, Lee, Soo Rang, Jeong, Namhee, Choi, Man Soo, Kim, Namshin, Kang, Sung-Taeg, and Park, Euiho
- Published
- 2019
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6. High-density linkage map reveals QTL for Type-I seed coat cracking in RIL population of soybean [Glycine max (L.) Merr.]
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Kang, Beom Kyu, Seo, Jeong Hyun, Jo, Hyun, Kulkarni, Krishnanand P., Choi, Man Soo, Kim, Hyun Tae, Lee, Jeong Dong, Dhungana, Sanjeev K., Kim, Hong Sik, Oh, Jae Hyeon, Park, Ji Hee, Shin, Sang Ouk, and Baek, In Youl
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- 2020
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7. QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean.
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Park, Hye Rang, Seo, Jeong Hyun, Kang, Beom Kyu, Kim, Jun Hoi, Heo, Su Vin, Choi, Man Soo, Ko, Jee Yeon, and Kim, Choon Song
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SEED proteins ,LOCUS (Genetics) ,SOY proteins ,SUCROSE ,SOYBEAN ,SINGLE nucleotide polymorphisms ,GENES - Abstract
This study aimed to discover the quantitative trait loci (QTL) associated with a high seed protein content in soybean and unravel the potential candidate genes. We developed two recombinant inbred line populations: YS and SI, by crossing Saedanbaek (high protein) with YS2035-B-91-1-B-1 (low protein) and Saedanbaek with Ilmi (low protein), respectively, and evaluated the protein content for three consecutive years. Using single-nucleotide polymorphism (SNP)-marker-based linkage maps, four QTLs were located on chromosomes 15, 18, and 20 with high logarithm of odds values (5.9–55.0), contributing 5.5–66.0% phenotypic variance. In all three experimental years, qPSD20-1 and qPSD20-2 were stable and identified in overlapping positions in the YS and SI populations, respectively. Additionally, novel QTLs were identified on chromosomes 15 and 18. Considering the allelic sequence variation between parental lines, 28 annotated genes related to soybean seed protein—including starch, lipid, and fatty acid biosynthesis-related genes—were identified within the QTL regions. These genes could potentially affect protein accumulation during seed development, as well as sucrose and oil metabolism. Overall, this study offers insights into the genetic mechanisms underlying a high soybean protein content. The identified potential candidate genes can aid marker-assisted selection for developing soybean lines with an increased protein content. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Intra-host competition and interactions between 'Soybean mosaic virus' (SMV) strains in mixed-infected soybean
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Hwang, Tae-Young, Jeong, Soon-Chun, Kim, Oksun, Park, Hyang-Mi, Lee, Seuk-Ki, Seo, Min-Jung, Choi, Man-Soo, Lee, Yu-Young, Yun, Hong-Tai, Kwon, Young-Up, Kim, Wook Han, and Kim, Yul-Ho
- Published
- 2011
9. A Genome-Wide Association Study of Protein, Oil, and Amino Acid Content in Wild Soybean (Glycine soja).
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Kim, Woon Ji, Kang, Byeong Hee, Kang, Sehee, Shin, Seoyoung, Chowdhury, Sreeparna, Jeong, Soon-Chun, Choi, Man-Soo, Park, Soo-Kwon, Moon, Jung-Kyung, Ryu, Jaihyunk, and Ha, Bo-Keun
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GENOME-wide association studies ,AMINO acids ,SOYBEAN ,PLANT proteins ,PROTEINS ,GLYCINE ,SOYBEAN farming - Abstract
Soybean (Glycine max L.) is a globally important source of plant proteins, oils, and amino acids for both humans and livestock. Wild soybean (Glycine soja Sieb. and Zucc.), the ancestor of cultivated soybean, could be a useful genetic source for increasing these components in soybean crops. In this study, 96,432 single-nucleotide polymorphisms (SNPs) across 203 wild soybean accessions from the 180K Axiom
® Soya SNP array were investigated using an association analysis. Protein and oil content exhibited a highly significant negative correlation, while the 17 amino acids exhibited a highly significant positive correlation with each other. A genome-wide association study (GWAS) was conducted on the protein, oil, and amino acid content using the 203 wild soybean accessions. A total of 44 significant SNPs were associated with protein, oil, and amino acid content. Glyma.11g015500 and Glyma.20g050300, which contained SNPs detected from the GWAS, were selected as novel candidate genes for the protein and oil content, respectively. In addition, Glyma.01g053200 and Glyma.03g239700 were selected as novel candidate genes for nine of the amino acids (Ala, Asp, Glu, Gly, Leu, Lys, Pro, Ser, and Thr). The identification of the SNP markers related to protein, oil, and amino acid content reported in the present study is expected to help improve the quality of selective breeding programs for soybeans. [ABSTRACT FROM AUTHOR]- Published
- 2023
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10. Genome-Wide Association Study for Agronomic Traits in Wild Soybean (Glycine soja).
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Kim, Woon Ji, Kang, Byeong Hee, Moon, Chang Yeok, Kang, Sehee, Shin, Seoyoung, Chowdhury, Sreeparna, Jeong, Soon-Chun, Choi, Man-Soo, Park, Soo-Kwon, Moon, Jung-Kyung, and Ha, Bo-Keun
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GENOME-wide association studies ,LINKAGE disequilibrium ,GENETIC variation ,GLYCINE ,PRINCIPAL components analysis ,SOYBEAN - Abstract
The agronomic traits of soybean are important because they are directly or indirectly related to its yield. Cultivated soybean (Glycine max (L.) Merr) has lost genetic diversity during domestication and selective breeding. However, wild soybean (G. soja) represents a useful breeding material because it has a diverse gene pool. In this study, a total of 96,432 single-nucleotide polymorphisms (SNPs) across 203 wild soybean accessions from the 180K Axiom
® Soya SNP array were employed in the association analysis. Wild soybean accessions were divided into four clusters based on their genetic distance using ADMIXTURE, principal component analysis, and neighbor-joining clusters. The linkage disequilibrium decayed rapidly in wild soybean. A genome-wide association study was conducted for days to flowering (DtF), days to maturity (DtM), the number of pods (NoP), and the 100-seed weight (100SW), which are major agronomic traits for wild soybean accessions. A total of 22 significant SNPs were found to be associated with DtF, DtM, and the 100SW. Based on the detected SNP markers, Glyma.12g210400, a gene related to DtF, Glyma.17g115300, a gene related to DtM, and Glyma.14g140200, a gene related to the 100SW, were selected as candidate genes. The SNP markers related to agronomic traits identified in this study are expected to help improve the quality of soybean cultivars through selective breeding. [ABSTRACT FROM AUTHOR]- Published
- 2023
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11. Quantitative Trait Loci (QTL) Analysis of Seed Protein and Oil Content in Wild Soybean (Glycine soja).
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Kim, Woon Ji, Kang, Byeong Hee, Moon, Chang Yeok, Kang, Sehee, Shin, Seoyoung, Chowdhury, Sreeparna, Choi, Man-Soo, Park, Soo-Kwon, Moon, Jung-Kyung, and Ha, Bo-Keun
- Subjects
LOCUS (Genetics) ,SEED proteins ,PROTEIN analysis ,AMINO acid sequence ,GLYCINE ,SOYBEAN - Abstract
Soybean seeds consist of approximately 40% protein and 20% oil, making them one of the world's most important cultivated legumes. However, the levels of these compounds are negatively correlated with each other and regulated by quantitative trait loci (QTL) that are controlled by several genes. In this study, a total of 190 F
2 and 90 BC1 F2 plants derived from a cross of Daepung (Glycine max) with GWS-1887 (G. soja, a source of high protein), were used for the QTL analysis of protein and oil content. In the F2:3 populations, the average protein and oil content was 45.52% and 11.59%, respectively. A QTL associated with protein levels was detected at Gm20_29512680 on chr. 20 with a likelihood of odds (LOD) of 9.57 and an R2 of 17.2%. A QTL associated with oil levels was also detected at Gm15_3621773 on chr. 15 (LOD: 5.80; R2 : 12.2%). In the BC1 F2:3 populations, the average protein and oil content was 44.25% and 12.14%, respectively. A QTL associated with both protein and oil content was detected at Gm20_27578013 on chr. 20 (LOD: 3.77 and 3.06; R2 15.8% and 10.7%, respectively). The crossover to the protein content of BC1 F3:4 population was identified by SNP marker Gm20_32603292. Based on these results, two genes, Glyma.20g088000 (S-adenosyl-l-methionine-dependent methyltransferases) and Glyma.20g088400 (oxidoreductase, 2-oxoglutarate-Fe(II) oxygenase family protein), in which the amino acid sequence had changed and a stop codon was generated due to an InDel in the exon region, were identified. [ABSTRACT FROM AUTHOR]- Published
- 2023
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12. Biochemical and proteomic analysis of soybean sprouts at different germination temperatures
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Koo, Sung Cheol, Kim, Sang Gon, Bae, Dong-Won, Kim, Hyun Young, Kim, Hyun Tae, Lee, Young Hoon, Kang, Beom Kyu, Baek, Seong-Bum, Baek, In Youl, Yun, Hong Tai, and Choi, Man Soo
- Published
- 2015
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13. Constitutive expression of mammalian nitric oxide synthase in tobacco plants triggers disease resistance to pathogens
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Chun, Hyun Jin, Park, Hyeong Cheol, Koo, Sung Cheol, Lee, Ju Huck, Park, Chan Young, Choi, Man Soo, Kang, Chang Ho, Baek, Dongwon, Cheong, Yong Hwa, Yun, Dae-Jin, Chung, Woo Sik, Cho, Moo Je, and Kim, Min Chul
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- 2012
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14. Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated Rice Stripe Virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene
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Park, Hyang-Mi, Choi, Man-Soo, Kwak, Do-Yeon, Lee, Bong-Choon, Lee, Jong-Hee, Kim, Myeong-Ki, Kim, Yeon-Gyu, Shin, Dong-Bum, Park, Soon-Ki, and Kim, Yul-Ho
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- 2012
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15. Proteomic Analysis of Seed Storage Proteins in Low Allergenic Soybean Accession
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Koo, Sung Cheol, Bae, Dong Won, Seo, Jun Su, Park, Kyoung Mi, Choi, Man Soo, Kim, Seok Hyeon, Shim, Sang In, Kim, Kyeong Moon, Chung, Jong Il, and Kim, Min Chul
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- 2011
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16. Expression of BrD1, a plant defensin from Brassica rapa, confers resistance against brown planthopper (Nilaparvata lugens) in transgenic rices
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Choi, Man-Soo, Kim, Yul-Ho, Park, Hyang-Mi, Seo, Bo-Yoon, Jung, Jin-Kyo, Kim, Sun-Tae, Kim, Min-Chul, Shin, Dong-Bum, Yun, Hong-Tai, Choi, Im-Soo, Kim, Chung-Kon, and Lee, Jang-Yong
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- 2009
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17. The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice
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Koo, Sung Cheol, Choi, Man Soo, Chun, Hyun Jin, Shin, Dong Bum, Park, Bong Soo, Kim, Yul Ho, Park, Hyang-Mi, Seo, Hak Soo, Song, Jong Tae, Kang, Kyu Young, Yun, Dae-Jin, Chung, Woo Sik, Cho, Moo Je, and Kim, Min Chul
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- 2009
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18. Identification and characterization of alternative promoters of the rice MAP kinase gene OsBWMK1
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Koo, Sung Cheol, Choi, Man Soo, Chun, Hyun Jin, Park, Hyeong Cheol, Kang, Chang Ho, Shim, Sang In, Chung, Jong Il, Cheong, Yong Hwa, Lee, Sang Yeol, Yun, Dae-Jin, Chung, Woo Sik, Cho, Moo Je, and Kim, Min Chul
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- 2009
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19. Analysis of Vertical Wind Shear Effects on Offshore Wind Energy Prediction Accuracy Applying Rotor Equivalent Wind Speed and the Relationship with Atmospheric Stability.
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Ryu, Geon Hwa, Kim, Dongjin, Kim, Dae-Young, Kim, Young-Gon, Kwak, Sung Jo, Choi, Man Soo, Jeon, Wonbae, Kim, Bum-Suk, and Moon, Chae-Joo
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VERTICAL wind shear ,WIND speed ,WIND power ,ATMOSPHERIC boundary layer ,WIND turbine aerodynamics ,TROPICAL cyclones - Abstract
If the wind speed that passed through a wind turbine rotor disk area is constant, the hub height wind speed (HHWS) could be representative of the wind speed over the rotor disk area. However, this assumption cannot be applied to the large wind turbine, because of the wind shear effect by atmospheric stability. This is because the hub height wind speed cannot represent the vertical wind shear effect from the aerodynamics characteristic on the wind turbine. Using SCADA and offshore LiDAR observation data of the Anholt offshore wind farm, it is investigated whether the rotor equivalent wind speed (REWS) introduced in IEC61400-12-1 can contribute to the improvement of power output forecasting accuracy. The weighted value by separated sector area and vertical wind shear effect by difference between heights can explain the role of energy flux and atmospheric stability on the exact wind energy calculation. The commercial CFD model WindSim is used to calculate power production according to the HHWS and the REWS, and to compare them with the actual AEP of the local wind farm. The classification of atmospheric stability is carried out by Richardson number, which well represents the thermal and physical properties of the atmosphere below the atmospheric boundary layer, along with the wind shear coefficient and turbulence intensity. When atmospheric stability was classified by each stability index, the REWS-based predicted power output was sometimes more accurate than HHWS, but sometimes inferior. However, in most cases, using the REWS, it was possible to calculate an estimate closer to the actual power output. Through the results of this study, it is possible to provide a rationale for which method, REWS or HHWS, can more accurately calculate the expected power output and effectively derive the economic feasibility of the project by identifying the characteristics of local atmospheric stability before the wind farm project. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Structural and chemical characterization of SiO2/TiO2 multicomponent particles during aerosol formation in a coflow diffusion flame
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Suh, Jeong Soo, Kim, Chan Soo, Kim, Tae Oh, and Choi, Man Soo
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- 2006
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21. Isolation of a Calmodulin-binding Transcription Factor from Rice (Oryza sativa L.)
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Choi, Man Soo, Kim, Min Chul, Yoo, Jae Hyuk, Moon, Byeong Cheol, Koo, Sung Cheol, Park, Byung Ouk, Lee, Ju Huck, Koo, Yoon Duck, Han, Hay Ju, Lee, Sang Yeol, Chung, Woo Sik, Lim, Chae Oh, and Cho, Moo Je
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- 2005
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22. Arachis hypogaea resveratrol synthase 3 alters the expression pattern of UDP-glycosyltransferase genes in developing rice seeds.
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Lee, Choonseok, Hong, Woo-Jong, Jung, Ki-Hong, Hong, Ha-Cheol, Kim, Dool-Yi, Ok, Hyun-Choong, Choi, Man-Soo, Park, Soo-Kwon, Kim, Jaehyun, and Koh, Hee-Jong
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RICE seeds ,RESVERATROL ,ARACHIS ,TRANSGENE expression ,RICE ,CULTIVARS ,PEANUTS - Abstract
The resveratrol-producing rice (Oryza sativa L.) inbred lines, Iksan 515 (I.515) and Iksan 526 (I.526), developed by the expression of the groundnut (Arachis hypogaea) resveratrol synthase 3 (AhRS3) gene in the japonica rice cultivar Dongjin, accumulated both resveratrol and its glucoside, piceid, in seeds. Here, we investigated the effect of the AhRS3 transgene on the expression of endogenous piceid biosynthesis genes (UGTs) in the developing seeds of the resveratrol-producing rice inbred lines. Ultra-performance liquid chromatography (UPLC) analysis revealed that I.526 accumulates significantly higher resveratrol and piceid in seeds than those in I.515 seeds and, in I.526 seeds, the biosynthesis of resveratrol and piceid reached peak levels at 41 days after heading (DAH) and 20 DAH, respectively. Furthermore, RNA-seq analysis showed that the expression patterns of UGT genes differed significantly between the 20 DAH seeds of I.526 and those of Dongjin. Quantitative real-time PCR (RT-qPCR) analyses confirmed the data from RNA-seq analysis in seeds of Dongjin, I.515 and I.526, respectively, at 9 DAH, and in seeds of Dongjin and I.526, respectively, at 20 DAH. A total of 245 UGTs, classified into 31 UGT families, showed differential expression between Dongjin and I.526 seeds at 20 DAH. Of these, 43 UGTs showed more than 2-fold higher expression in I.526 seeds than in Dongjin seeds. In addition, the expression of resveratrol biosynthesis genes (PAL, C4H and 4CL) was also differentially expressed between Dongjin and I.526 developing seeds. Collectively, these data suggest that AhRS3 altered the expression pattern of UGT genes, and PAL, C4H and 4CL in developing rice seeds. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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23. Stabilizing decontamination foam using surface-modified silica nanoparticles containing chemical reagent: foam stability, structures, and dispersion properties.
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Yoon, In-Ho, Yoon, Suk Bon, Sihn, Youngho, Choi, Man-Soo, Jung, Chong-Hun, and Choi, Wang-Kyu
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- 2021
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24. Korean soybean core collection: Genotypic and phenotypic diversity population structure and genome-wide association study.
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Jeong, Namhee, Kim, Ki-Seung, Jeong, Seongmun, Kim, Jae-Yoon, Park, Soo-Kwon, Lee, Ju Seok, Jeong, Soon-Chun, Kang, Sung-Taeg, Ha, Bo-Keun, Kim, Dool-Yi, Kim, Namshin, Moon, Jung-Kyung, and Choi, Man Soo
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SOYBEAN ,FOOD crops ,COLLECTING of accounts ,COLLECTIONS ,ANALYSIS of variance - Abstract
A core collection is a subset that represents genetic diversity of the total collection. Soybean (Glycine max (L.) Merr.) is one of major food and feed crops. It is the world's most cultivated annual herbaceous legume. Constructing a core collection for soybean could play a pivotal role in conserving and utilizing its genetic variability for research and breeding programs. To construct and evaluate a Korean soybean core collection, genotypic and phenotypic data as well as population structure, were analyzed. The Korean soybean core collection consisted of 430 accessions selected from 2,872 collections based on Affymetrix Axiom
® 180k SoyaSNP array data. The core collection represented 99% of genotypic diversity of the total collection. Analysis of population structure clustered the core collection into five subpopulations. Accessions from South Korea and North Korea were distributed across five subpopulations. Analysis of molecular variance indicated that only 2.01% of genetic variation could be explained by geographic origins while 16.18% of genetic variation was accounted for by subpopulations. Genome-wide association study (GWAS) for days to flowering, flower color, pubescent color, and growth habit confirmed that the core collection had the same genetic diversity for tested traits as the total collection. The Korean soybean core collection was constructed based on genotypic information of the 180k SNP data. Size and phenotypic diversity of the core collection accounted for approximately 14.9% and 18.1% of the total collection, respectively. GWAS of core and total collections successfully confirmed loci associated with tested traits. Consequently, the present study showed that the Korean soybean core collection could provide fundamental and practical material and information for both soybean genetic research and breeding programs. [ABSTRACT FROM AUTHOR]- Published
- 2019
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25. Quantitative trait locus analysis for soybean (Glycine max) seed protein and oil concentrations using selected breeding populations.
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Seo, Jeong‐Hyun, Kim, Ki‐Seung, Ko, Jong‐Min, Choi, Man‐Soo, Kang, Beom‐Kyu, Kwon, Soon‐Wook, Jun, Tae‐Hwan, and Singh, Ram
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SEED proteins ,SOY oil ,PLANT breeding ,SINGLE nucleotide polymorphisms ,LOCUS in plant genetics - Abstract
Soybean seed protein and oil concentrations are important traits that directly affect the quality of soyfoods. Many studies and breeding programmes have been conducted to find major quantitative trait loci (QTL) that regulate protein and oil concentrations and to develop soybean cultivars with high protein and/or oil content. The purpose of this study was to identify these QTL using a selected breeding population. The population was tested in field conditions over a period of 3 years. Seed protein and oil concentrations were measured each year. Single‐nucleotide polymorphisms (SNPs) were used to construct genetic map using a 180K SoyaSNP array, which identified 1,570 SNPs. We identified 12 QTL for seed protein, 11 for seed oil concentration and four for both traits. Among these, 17 QTL were closely mapped to previously reported QTL, whereas ten sites were novel. Several QTL were detected across at least two experimental years. These loci are good candidate QTL for optimal seed protein and oil concentrations. Our results demonstrate that favourable target QTL can be successfully identified using selected breeding populations. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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26. Computer-aided design models for millimeter-wave suspended-substrate microstrip line
- Author
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Choi, Man Soo., Atwater, H.A., Lee, H. M., Naval Postgraduate School (U.S.), and Department of Electrical and Computer Engineering
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Radar circuits ,Strip transmission lines ,Millimeter wave ,design model ,suspended substrate ,Microwave circuits - Abstract
An equivalent circuit model was derived for the series gap discontinuity in shielded suspended-substrate transmission line. Numerical values of the circuit parameters were computed for various sets of line dimensions, over a range of operating frequencies. The shielded suspended-substrate line is a transmission medium useful for radar and microwave circuits in the Ka band frequency range, 30 - 40 GHz. In order to utilize this transmission medium in the construction of microwave circuits and filters, it is necessary to have valid circuit models for typical discontinuous change in width. These discontinuity characteristics may be deduced on the basis of a calculation of their scattering coefficients for propagating waves in the medium. The approach chosen in this thesis, however, is that of placing the selected discontinuity in an open-ended strip resonator in the transmission medium. An appropriate circuit model is adopted for the given discontinuity, and its circuit elements are then deduced on the basis of the perturbation of the resonance frequency of the strip resonator which is induced by the mode. The boundary-value problem associated with the microstrip resonator structure has been approached in a rigorous manner based on a full-wave analysis which utilizes a process of solving the electromagnetic (EM) boundary value problem with inclusion of all the field components. http://archive.org/details/computeraideddes1094534845 Lieutenant, Republic of Korea Navy Approved for public release; distribution is unlimited.
- Published
- 1990
27. Planar laser light scattering technique for measurement of nonspherical particles.
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Kim, Hyun Woo, Choi, Man Soo, Jeong, Dae Hwa, and Lee, Hyo Hyung
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- 2004
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28. Enhanced electrochemical performance of Li[sub 3]V[sub 2](PO[sub 4])[sub 3]/Ag-graphene composites as cathode materials for Li-ion batteries.
- Author
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Choi, Man-Soo, Kim, Hyun-Soo, Lee, Young-Moo, and Jin, Bong-Soo
- Abstract
In this study, we have synthesized a Li[sub 3]V[sub 2](PO[sub 4])[sub 3]/Ag-graphene (LVP/Ag-G) composite using a facile sol-gel route. The physical and electrochemical properties of the LVP/Ag-G composite have been evaluated by X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy and electrochemical measurements. In the potential range 3.0-4.3 V, the LVP/Ag-G electrode delivered a capacity of 135 mA h g[sup -1] at a rate of 0.1 C, which is above the theoretical capacity (133 mA h g[sup -1]). Even at a high current rate of 10 C, it still exhibited a discharge capacity of 118 and 133 mA h g[sup -1] in the potential ranges 3.0-4.3 and 3.0-4.8 V, respectively. Such a capacity value is significantly higher than previously reported works. The LVP/Ag-G composites exhibited an outstanding specific capacity, rate capability and cycling stability. These exceptional properties of the LVP/Ag-G composites were mainly attributed to the synergetic effect of the graphene and the silver particles. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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29. Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin
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Moon, Byeong Cheol, Choi, Man Soo, Kang, Yun Hwan, Kim, Min Chul, Cheong, Mi Sun, Park, Chan Young, Yoo, Jae Hyuk, Koo, Sung Cheol, Lee, Sang Min, Lim, Chae Oh, Cho, Moo Je, and Chung, Woo Sik
- Published
- 2005
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30. WRKY group IId transcription factors interact with calmodulin
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Park, Chan Young, Lee, Ju Huck, Yoo, Jae Hyuk, Moon, Byeong Cheol, Choi, Man Soo, Kang, Yun Hwan, Lee, Sang Min, Kim, Ho Soo, Kang, Kyu Young, Chung, Woo Sik, Lim, Chae Oh, and Cho, Moo Je
- Published
- 2005
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31. Atmospheric Stability Effects on Offshore and Coastal Wind Resource Characteristics in South Korea for Developing Offshore Wind Farms.
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Ryu, Geon Hwa, Kim, Young-Gon, Kwak, Sung Jo, Choi, Man Soo, Jeong, Moon-Seon, and Moon, Chae-Joo
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OFFSHORE wind power plants ,ATMOSPHERIC boundary layer ,WIND shear ,SEA breeze ,METEOROLOGICAL observations ,COASTS ,INDUCTION generators - Abstract
South Korea is surrounded by the sea on three sides. The characteristics of offshore wind resources vary from region to region due to the influence of the distribution of the coastline and differences in roughness length and atmospheric stability between the coast and the sea. In particular, turbulent gusts and low-level wind shear occurring near the hub height of the wind turbine within the atmospheric boundary layer have a significant effect on the load of wind turbines. These severe weather phenomena are closely related to atmospheric stability. Therefore, the objective of this study is to determine differences in wind resource characteristics in the South Korean offshore and coast in relation to variations in atmospheric stability using observation data from the HeMOSU-1 meteorological tower in the West Sea and the Boseong meteorological observation tower on the southern coast. On the southern coast, changes in sea and land breezes are observed throughout diurnal and nocturnal periods, with an atmospheric stability distribution similar to that of land, which is unstable during the day and becomes more stable at night. On the other hand, the stable ratio continues to dominate in the west offshore. In the case of coastal areas, low-level wind shear occasionally occurs near the general wind turbine hub height approximately over 100 m due to the influence of winds from the sea. This study shows that when constructing an offshore wind farm, it is necessary to first analyze the characteristics of local coastal and offshore wind resources for more efficient and safe wind farm construction and operation. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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32. Analysis on the Magnetic Force Characteristics of Segmented Magnet Used in Large Permanent-Magnet Wind Power Generator.
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Jang, Seok-Myeong, Park, Hyun-Jun, Choi, Ji-Hwan, Han, Cheol, and Choi, Man-Soo
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MAGNETISM ,PERMANENT magnet motors ,ELECTROMAGNETS ,WIND power ,MAGNETOMECHANICAL effects ,MAGNETIC multilayers ,ELECTRIC machinery rotors ,MAGNETIC flux - Abstract
It is important to increase the production efficiency of large wind power generators. Because magnet segmentation reduces the size of each magnet, the segmented magnets can be fabricated relatively easily. It also features the advantages of reducing the cogging torque and improving the efficiency of permanent-magnet machines by decreasing the eddy-current loss. Despite this benefit, a disadvantage exists as the side segmented magnets could be detached from the rotor because of the magnetic force between the magnets, such as the attractive force or repulsive force. Hence, in this study, we will analyze the characteristics of the magnetic force acting on the segmented magnets and predict the events they can possibly cause. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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33. QTL Mapping and Candidate Gene Analysis for Pod Shattering Tolerance in Soybean (Glycine max).
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Seo, Jeong-Hyun, Kang, Beom-Kyu, Dhungana, Sanjeev K., Oh, Jae-Hyeon, Choi, Man-Soo, Park, Ji-Hee, Shin, Sang-Ouk, Kim, Hong-Sik, Baek, In-Youl, Sung, Jung-Sook, Jung, Chan-Sik, Kim, Ki-Seung, and Jun, Tae-Hwan
- Subjects
GENE mapping ,SINGLE nucleotide polymorphisms ,CHROMOSOMES ,GENOMES ,ALLELES - Abstract
Pod shattering is an important reproductive process in many wild species. However, pod shattering at the maturing stage can result in severe yield loss. The objectives of this study were to discover quantitative trait loci (QTLs) for pod shattering using two recombinant inbred line (RIL) populations derived from an elite cultivar having pod shattering tolerance, namely "Daewonkong", and to predict novel candidate QTL/genes involved in pod shattering based on their allele patterns. We found several QTLs with more than 10% phenotypic variance explained (PVE) on seven different chromosomes and found a novel candidate QTL on chromosome 16 (qPS-DS16-1) from the allele patterns in the QTL region. Out of the 41 annotated genes in the QTL region, six were found to contain SNP (single-nucleotide polymorphism)/indel variations in the coding sequence of the parents compared to the soybean reference genome. Among the six potential candidate genes, Glyma.16g076600, one of the genes with known function, showed a highly differential expression levels between the tolerant and susceptible parents in the growth stages R3 to R6. Further, Glyma.16g076600 is a homolog of AT4G19230 in Arabidopsis, whose function is related to abscisic acid catabolism. The results provide useful information to understand the genetic mechanism of pod shattering and could be used for improving the efficiency of marker-assisted selection for developing varieties of soybeans tolerant to pod shattering. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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34. High Throughput Phenotyping for Various Traits on Soybean Seeds Using Image Analysis.
- Author
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BAEK, JeongHo, Lee, Eungyeong, Kim, Nyunhee, Kim, Song Lim, Choi, Inchan, Ji, Hyeonso, Chung, Yong Suk, Choi, Man-Soo, Moon, Jung-Kyung, and Kim, Kyung-Hwan
- Subjects
IMAGE analysis ,SEEDS ,PHANEROGAMS ,ANALYSIS of colors ,INFORMATION storage & retrieval systems - Abstract
Data phenotyping traits on soybean seeds such as shape and color has been obscure because it is difficult to define them clearly. Further, it takes too much time and effort to have sufficient number of samplings especially length and width. These difficulties prevented seed morphology to be incorporated into efficient breeding program. Here, we propose methods for an image acquisition, a data processing, and analysis for the morphology and color of soybean seeds by high-throughput method using images analysis. As results, quantitative values for colors and various types of morphological traits could be screened to create a standard for subsequent evaluation of the genotype. Phenotyping method in the current study could define the morphology and color of soybean seeds in highly accurate and reliable manner. Further, this method enables the measurement and analysis of large amounts of plant seed phenotype data in a short time, which was not possible before. Fast and precise phenotype data obtained here may facilitate Genome Wide Association Study for the gene function analysis as well as for development of the elite varieties having desirable seed traits. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
35. GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill).
- Author
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Shim, Sangrea, Ha, Jungmin, Kim, Moon Young, Choi, Man Soo, Kang, Sung-Taeg, Jeong, Soon-Chun, Moon, Jung-Kyung, and Lee, Suk-Ha
- Subjects
SOYBEAN ,GLYCINE ,GENE expression ,SINGLE nucleotide polymorphisms ,TRANSCRIPTION factors - Abstract
Branch number is one of the main factors affecting the yield of soybean (Glycine max (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs) were associated with branch numbers in a soybean core collection. Among these QTNs, a linkage disequilibrium (LD) block qtnBR6-1 spanning 20 genes was found to overlap a previously identified major quantitative trait locus qBR6-1. To validate and narrow down qtnBR6-1, we developed a set of near-isogenic lines (NILs) harboring high-branching (HB) and low-branching (LB) alleles of qBR6-1, with 99.96% isogenicity and different branch numbers. A cluster of single nucleotide polymorphisms (SNPs) segregating between NIL-HB and NIL-LB was located within the qtnBR6-1 LD block. Among the five genes showing differential expression between NIL-HB and NIL-LB, BRANCHED1 (BRC1; Glyma.06G210600) was down-regulated in the shoot apex of NIL-HB, and one missense mutation and two SNPs upstream of BRC1 were associated with branch numbers in 59 additional soybean accessions. BRC1 encodes TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 and 2 transcription factor and functions as a regulatory repressor of branching. On the basis of these results, we propose BRC1 as a candidate gene for branching in soybean. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
36. The discovery of novel SNPs associated with group A soyasaponin biosynthesis from Korea soybean core collection.
- Author
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Lee, Sang-Beom, Lee, Kwang-Sik, Kim, Hyun-Young, Kim, Dool-Yi, Seo, Mi-Suk, Jeong, Soon-Chun, Moon, Jung-Kyung, Park, Soo-Kwon, and Choi, Man-Soo
- Subjects
- *
STEROIDAL alkaloids , *BIOSYNTHESIS , *SINGLE nucleotide polymorphisms , *SOYBEAN , *SOYBEAN products - Abstract
Soyasaponin is a type of glycoside such as steroids, steroidal alkaloids or triterpenes, which enhance the body immunity. In order to efficiently identify genes and markers related to the soyasaponin, we used a 180K Axiom® SoyaSNP array and whole genome resequencing data from the Korean soybean core collection. As a result of conducting GWAS for group A soyasaponin (Aa and Ab derivatives), 16 significant common markers associated with Aa and Ab derivatives were mapped to chromosome 7, and three candidate genes including Glyma.07g254600 were detected. The functional haplotypes for candidate genes showed that Aa and Ab contents were mainly determined by alleles of AX-90322128, the marker of Glyma.07g254600. In addition, 14 novel SNPs variants closely associated with Aa and Ab derivatives were discovered for Glyma.07g254600. Therefore, the results of this study that identified soyasaponin-associated markers and useful genes utilizing various genomic information could provide insight into functional soybean breeding. • Group A soyasaponin induces a bitter and astringent taste in soy products, but function to prevent liver damage. • Aa and Ab derivatives of group A soyasaponins were highly associated with genetic factors. • Aa and Ab derivatives of group A soyasaponins were determined by Glyma.07g254600. • Combination of SNP chip with resequencing whole genome enables the identification of markers associated with major traits. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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37. Comparing extraction methods for the determination of tocopherols and tocotrienols in seeds and germinating seeds of soybean transformed with OsHGGT
- Author
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Lee, Yu Young, Park, Hyang Mi, Lee, Choon Ki, Kim, Sun Lim, Hwang, Tae-Young, Choi, Man Soo, Kwon, Young-Up, Kim, Wook Han, Kim, Si Ju, Lee, Sang Chul, and Kim, Yul Ho
- Subjects
- *
VITAMIN E , *EXTRACTION techniques , *COMPARATIVE studies , *TOCOTRIENOL , *GERMINATION , *SOYBEAN - Abstract
Abstract: Previously, transgenic soybeans were generated and reported to produce tocotrienols (α-, γ- and δ-tocotrienols), compounds not normally found in soybean. Three procedures were evaluated to optimize the extraction method for transgenic soybean seeds and germinating seeds. Significant differences were observed among the extraction methods in seeds and germinating seeds. In seeds, the highest analytical values (tocopherols, 37.11mg100g−1; and tocotrienols, 1.54mg100g−1) were observed by using rapid Soxhlet extraction. In germinating seeds, the content of transgenic soybean (B20 and C5) total vitamin E (tocopherols, 18.04, 20.73mg100g−1; and tocotrienols, 0.82 and 0.84mg100g−1) by direct extraction was approximately 16% and 9% greater than the amount obtained by saponification. In addition, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) tests demonstrated a direct correlation between the radical-scavenging capacity and the total content of natural tocopherols and tocotrienols. Therefore, these results suggest that an optimal extraction method will provide a fast, simple, reproducible procedure for analyzing tocotrienols and tocopherols. Furthermore, this method may be used to determine novel minor functional compounds such as tocotrienols for the evaluation of biological activity. [Copyright &y& Elsevier]
- Published
- 2012
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38. An S-locus receptor-like kinase plays a role as a negative regulator in plant defense responses
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Kim, Ho Soo, Jung, Mi Soon, Lee, Sang Min, Kim, Kyung Eun, Byun, Hyejin, Choi, Man Soo, Park, Hyeong Cheol, Cho, Moo Je, and Chung, Woo Sik
- Subjects
- *
PLANT defenses , *CELLULAR signal transduction , *ARABIDOPSIS , *PROTEIN kinases , *TRANSGENIC plants , *NATURAL immunity , *PATHOGENIC microorganisms - Abstract
Abstract: Plant cells often use cell surface receptors to sense environmental changes and then transduce external signals via activated signaling pathways to trigger adaptive responses. In Arabidopsis, the receptor-like protein kinase (RLK) gene family contains more than 600 members, and some of these are induced by pathogen infection, suggesting a possible role in plant defense responses. We previously characterized an S-locus RLK (CBRLK1) at the biochemical level. In this study, we examined the physiological function of CBRLK1 in defense responses. CBRLK1 mutant and CBRLK1-overexpressing transgenic plants showed enhanced and reduced resistance against a virulent bacterial pathogen, respectively. The altered pathogen resistances of the mutant and overexpressing transgenic plants were associated with increased and reduced induction of the pathogenesis-related gene PR1, respectively. These results suggest that CBRLK1 plays a negative role in the disease resistance signaling pathway in Arabidopsis. [Copyright &y& Elsevier]
- Published
- 2009
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39. Effects of genotype and environment on the nutrient and metabolic profiles of soybeans genetically modified with epidermal growth factor or thioredoxin compared with conventional soybeans.
- Author
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Kim, Ye Jin, Park, Young Jin, Oh, Sung-Dug, Yoon, Jeong Su, Kim, Jae Geun, Seo, Ju-Seok, Park, Jung-Ho, Kim, Chang-Gi, Park, Soo-Yun, Park, Soon Ki, Choi, Man-Soo, and Kim, Jae Kwang
- Subjects
- *
EPIDERMAL growth factor , *SOYBEAN , *THIOREDOXIN , *METABOLITES , *SUCROSE , *GENOTYPES , *POLYMER networks - Abstract
Genetically modified (GM) soybean events CT-4025 and CT-1001, which contain genes encoding thioredoxin and epidermal growth factor, respectively, have previously been developed for use in cosmetic materials. In this study, compositional analyses and metabolic profiling were carried out to assess the unintended effects on the composition of metabolites between GM soybeans and non-GM soybeans. The soybeans were cultivated in 2016 and 2017 in two regions (Ochang and Jeonju) in the Republic of Korea. Statistical analyses were performed using univariate and multivariate methods. Results from the compositional analysis showed that the compositional variation in the tested GM varieties was within the range of conventional varieties. Furthermore, the results of multivariate analysis revealed that the growing year had a much stronger effect on soybean composition than did the growing site or genotypes. In the case of soybeans harvested in 2016, the levels of most amino acids decreased while those of secondary metabolites and sucrose increased, indicating that water stress due to low rainfall in 2016 induced metabolic changes. These results suggest that environmental factors have a major impact on the composition of GM and non-GM soybeans. We further demonstrated that metabolic approaches are useful for investigating the environmental change-induced metabolic network in GM crops intended for use in cosmetic materials. [Display omitted] • GMO soybeans intended for cosmetic use are compositionally equivalent to non-GMOs. • Environmental factors cause more differences than genetic modification. • Metabolomic approaches can determine compositional changes. • GMO soybeans with epidermal growth factor or thioredoxin are safe for cosmetic use. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
40. Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance
- Author
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Lim, Chan Ju, Hwang, Jung Eun, Chen, Huan, Hong, Joon Ki, Yang, Kyung Ae, Choi, Man Soo, Lee, Kyun Oh, Chung, Woo Sik, Lee, Sang Yeol, and Lim, Chae Oh
- Subjects
- *
PHYSIOLOGICAL effects of heat , *ARABIDOPSIS , *TRANSCRIPTION factors , *TRANSGENIC plants - Abstract
Abstract: The dehydration responsive element binding protein 2 (DREB2) subgroup belongs to the plant-specific APETALA2/ethylene-responsive element binding factor (AP2/ERF) family of transcription factors. We have characterized cDNA encoding Arabidopsis thaliana DREB2C, which is induced by mild heat stress. Both an electrophoretic mobility shift assay (EMSA) and a yeast one-hybrid assay revealed that DREB2C145–528 was able to form a complex with the dehydration responsive element/C-repeat (DRE/CRT; A/GCCGAC) motif. A trans-activating ability test in yeast demonstrated that DREB2C could effectively function as a trans-activator. Constitutive expression of DREB2C under the control of the cauliflower mosaic virus (CaMV) 35S promoter led to enhanced thermotolerance in transgenic lines of Arabidopsis. Microarray and RT-PCR analyses of transgenic plants revealed that DREB2C regulates expression of several heat stress-inducible genes that contain DRE/CRT elements in their promoters. From these data, we deduced that DREB2C is a regulator of heat stress tolerance in Arabidopsis. [Copyright &y& Elsevier]
- Published
- 2007
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41. Identification of New Isolates of Phytophthora sojae and Selection of Resistant Soybean Genotypes.
- Author
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Heo SV, Park HR, Jang YW, Park J, Kang BK, Seo JH, Kim JH, Lee JY, Choi MS, Ko JY, Kim CS, Lee S, and Jun TH
- Abstract
Phytophthora root and stem rot (PRR), caused by Phytophthora sojae, can occur at any growth stage under poorly drained and humid conditions. The expansion of soybean cultivation in South Korean paddy fields has increased the frequency of PRR outbreaks. This study aimed to identify four P. sojae isolates newly collected from domestic fields and evaluate race-specific resistance using the hypocotyl inoculation technique. The four isolates exhibited various pathotypes, with GJ3053 exhibiting the highest virulence complexity. Two isolates, GJ3053 and AD3617, were screened from 205 soybeans, and 182 and 190 genotypes (88.8 and 92.7%, respectively) were susceptible to each isolate. Among these accessions, five genotypes resistant to both isolates were selected. These promising genotypes are candidates for the development of resistant soybean cultivars that can effectively control PRR through gene stacking.
- Published
- 2024
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42. Flavobacterium endoglycinae sp. nov., an endophytic bacterium isolated from soybean ( Glycine max L. cv. Gwangan) stems.
- Author
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Seo J, Peng Y, Jiang L, Lee SB, Jeong RD, Park SJ, Kim CY, Choi MS, and Lee J
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Fatty Acids chemistry, Phospholipids chemistry, Plant Stems microbiology, RNA, Ribosomal, 16S genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2 analogs & derivatives, Vitamin K 2 chemistry, Flavobacterium classification, Flavobacterium isolation & purification, Phylogeny, Glycine max microbiology
- Abstract
A Gram-stain-negative, facultatively anaerobic, motile by gliding, rod-shaped, oxidase- and catalase-positive bacterial strain, designated BB8
T , was isolated from the stems of a Korean soybean cultivar ( Glycine max L. cv. Gwangan). The strain produced a yellow pigment on tryptic soy agar. Growth of strain BB8T occurred at pH 5.0-8.0 (optimum, pH 7.0), at 10-35 °C (optimum, 25-30 °C) and in the presence of 0-1 % (w/v) NaCl (optimum, 0.5%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BB8T formed a lineage within the genus Flavobacterium and was most closely related to Flavobacterium artemisiae SYP-B1015T (96.9 % 16S rRNA gene sequence similarity) and Flavobacterium ustbae T13T (96.8%). The complete genome sequence of strain BB8T was 5 513 159 bp long with a G+C content of 34.1 mol%. The major fatty acids (>10 %) of strain BB8T were iso-C15 : 0 (21 %), summed feature 3 (comprising C16 : 1 ω7 c and/or C16 : 1 ω6 c , 20.3%) and iso-C16 : 0 3-OH (13.7%). The predominant polar lipids were phosphatidylethanolamine and unidentified aminolipids, and the major respiratory quinone was menaquinone-6. Based on these phenotypic, genotypic and chemotaxonomic characteristics, strain BB8T is considered to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium endoglycinae sp. nov. is proposed. The type strain is BB8T (=KCTC 82167T =CCTCC AB 2020070T ).- Published
- 2022
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43. A Promising Na3V2(PO4)3/Ag + Graphene Composites as Cathode Material for Hybrid Lithium Batteries.
- Author
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Choi MS, Kim HS, Lee YM, Lee SM, and Jin BS
- Abstract
The NASICON (sodium super ionic conductor) based Na3V2(PO4)3/Ag + graphene (NVP/Ag + G) was successfully synthesized through a sol-gel route using a silver nitrate and graphene as a raw material. The effects of the physical and electrochemical properties of the NVP/Ag + G composites have been evaluated with X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy, and electrochemical measurements. The graphene and Ag significantly influenced the morphology, structure and electrochemical performance of the Na3V2(PO4)3 material. In the electrochemical measurement, the (NVP/Ag + G) electrode showed the discharge capacity of 102 mAh g(-1) at 0.1 C rate, which was higher than the pristine Na3V2(PO4). At a current rate of 5 C, it still exhibits the discharge capacity of 73 mAh g(-1) and the capacity retention of 71.6%. The results of higher electrochemical performance of the NVP/Ag + G composites are mainly attributed to the synergetic effect of the graphene and the silver particles.
- Published
- 2015
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44. Physical and electrochemical characterization of 0.3Li2MnO3 x 0.7LiMn0.60Ni0.25Co0.15O2 material for Li secondary battery.
- Author
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Kim JM, Choi MS, Jin BS, Wang G, and Kim HS
- Abstract
The 0.3Li2MnO3 x 0.7LiMn0.60Ni0.25Co0.15O2 cathode materials were synthesized using a coprecipitation method at a various heat-treatment temperature. From XRD pattern analysis, pure layered structure without impurities was confirmed from all samples and the peak intensity of Li2MnO3 was increased as the heat-treatment temperature increased. The primary particle size increased approximately from 100 nm to 500 nm with increasing heat-treatment temperature. The initial discharge capacity of the materials obtained at 950 degrees C was 235 mA h/g at 0.1 C rate, but then decreased down to 228 mA h/g with further increasing heat-treatment temperature. And, in the voltage range of 2.0-4.6 V, the electrode heat-treated at 900 degrees C showed the highest capacity retention of 68% at 5 C rate against to 0.1 C rate.
- Published
- 2014
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45. Variation block-based genomics method for crop plants.
- Author
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Kim YH, Park HM, Hwang TY, Lee SK, Choi MS, Jho S, Hwang S, Kim HM, Lee D, Kim BC, Hong CP, Cho YS, Kim H, Jeong KH, Seo MJ, Yun HT, Kim SL, Kwon YU, Kim WH, Chun HK, Lim SJ, Shin YA, Choi IY, Kim YS, Yoon HS, Lee SH, and Lee S
- Subjects
- Base Sequence, Chromosome Mapping, Plant Proteins genetics, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Sequence Analysis, DNA, Crops, Agricultural genetics, Genome, Plant, Glycine max genetics
- Abstract
Background: In contrast with wild species, cultivated crop genomes consist of reshuffled recombination blocks, which occurred by crossing and selection processes. Accordingly, recombination block-based genomics analysis can be an effective approach for the screening of target loci for agricultural traits., Results: We propose the variation block method, which is a three-step process for recombination block detection and comparison. The first step is to detect variations by comparing the short-read DNA sequences of the cultivar to the reference genome of the target crop. Next, sequence blocks with variation patterns are examined and defined. The boundaries between the variation-containing sequence blocks are regarded as recombination sites. All the assumed recombination sites in the cultivar set are used to split the genomes, and the resulting sequence regions are termed variation blocks. Finally, the genomes are compared using the variation blocks. The variation block method identified recurring recombination blocks accurately and successfully represented block-level diversities in the publicly available genomes of 31 soybean and 23 rice accessions. The practicality of this approach was demonstrated by the identification of a putative locus determining soybean hilum color., Conclusions: We suggest that the variation block method is an efficient genomics method for the recombination block-level comparison of crop genomes. We expect that this method will facilitate the development of crop genomics by bringing genomics technologies to the field of crop breeding.
- Published
- 2014
- Full Text
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46. Antioxidant activity and inhibition of lipid peroxidation in germinating seeds of transgenic soybean expressing OsHGGT.
- Author
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Kim YH, Lee YY, Kim YH, Choi MS, Jeong KH, Lee SK, Seo MJ, Yun HT, Lee CK, Kim WH, Lee SC, Park SK, and Park HM
- Subjects
- Alkyl and Aryl Transferases metabolism, Antioxidants pharmacology, Gene Expression, Germination, Plant Extracts pharmacology, Plant Proteins metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified growth & development, Plants, Genetically Modified metabolism, Seeds chemistry, Seeds genetics, Seeds growth & development, Seeds metabolism, Glycine max genetics, Glycine max growth & development, Glycine max metabolism, Alkyl and Aryl Transferases genetics, Antioxidants analysis, Lipid Peroxidation drug effects, Oryza enzymology, Plant Extracts analysis, Plant Proteins genetics, Plants, Genetically Modified chemistry, Glycine max chemistry
- Abstract
Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of β-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4-17 and 23.2-35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0-4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination.
- Published
- 2011
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47. Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in arabidopsis.
- Author
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Yoo JH, Park CY, Kim JC, Heo WD, Cheong MS, Park HC, Kim MC, Moon BC, Choi MS, Kang YH, Lee JH, Kim HS, Lee SM, Yoon HW, Lim CO, Yun DJ, Lee SY, Chung WS, and Cho MJ
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Binding Sites genetics, Calcium Signaling physiology, Calmodulin chemistry, DNA, Complementary, Gene Expression Regulation, Plant physiology, Isomerism, Mutagenesis, Site-Directed, Plants, Genetically Modified, Proline metabolism, Trans-Activators genetics, Ubiquitin, Yeasts, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Calmodulin genetics, Calmodulin metabolism, Salts metabolism, Trans-Activators metabolism
- Abstract
Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety of enzymes and proteins. Plants express numerous CaM isoforms that exhibit differential activation and/or inhibition of CaM-dependent enzymes in vitro. However, the specific biological functions of plant CaM are not well known. In this study, we isolated a cDNA encoding a CaM binding transcription factor, MYB2, that regulates the expression of salt- and dehydration-responsive genes in Arabidopsis. This was achieved using a salt-inducible CaM isoform (GmCaM4) as a probe from a salt-treated Arabidopsis expression library. Using domain mapping, we identified a Ca2+-dependent CaM binding domain in MYB2. The specific binding of CaM to CaM binding domain was confirmed by site-directed mutagenesis, a gel mobility shift assay, split ubiquitin assay, and a competition assay using a Ca2+/CaM-dependent enzyme. Interestingly, the specific CaM isoform GmCaM4 enhances the DNA binding activity of AtMYB2, whereas this was inhibited by a closely related CaM isoform (GmCaM1). Overexpression of Gm-CaM4 in Arabidopsis up-regulates the transcription rate of AtMYB2-regulated genes, including the proline-synthesizing enzyme P5CS1 (Delta1-pyrroline-5-carboxylate synthetase-1), which confers salt tolerance by facilitating proline accumulation. Therefore, we suggest that a specific CaM isoform mediates salt-induced Ca2+ signaling through the activation of an MYB transcriptional activator, thereby resulting in salt tolerance in plants.
- Published
- 2005
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48. Pathogenesis-related gene expression by specific calmodulin isoforms is dependent on NIM1, a key regulator of systemic acquired resistance.
- Author
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Park CY, Heo WD, Yoo JH, Lee JH, Kim MC, Chun HJ, Moon BC, Kim IH, Park HC, Choi MS, Ok HM, Cheong MS, Lee SM, Kim HS, Lee KH, Lim CO, Chung WS, and Cho MJ
- Subjects
- Arabidopsis immunology, Arabidopsis Proteins physiology, Calmodulin genetics, Gene Expression Regulation, Plant drug effects, Plants, Genetically Modified, Protein Isoforms, Glycine max genetics, Transcriptional Activation, Transfection, Calmodulin pharmacology, Gene Expression Regulation, Plant immunology, Immunity, Innate genetics
- Abstract
Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca2+ signals. This diversity results in different physiological responses to particular stimuli. Gm-CaM-4 and -5 are two divergent calmodulin isoforms from the soybean (Glycine max) that have been reported to be involved in plant disease resistance. However, little is known about the pathway by which these specific isoforms transduce the defense signal and up-regulate pathogenesis-related (PR) genes. Here we report that overexpression of GmCaM-4/-5 induces constitutive PR gene expression and enhances disease resistance in wild-type Arabidopsis, but not in the nim1 mutant of Arabidopsis. GmCaM-4/-5 also appear to activate trans-acting elements that bind to cis-acting elements in the Arabidopsis PR-1 promoter. Thus up-regulation of PR genes by these GmCaM isoforms is dependent on NIM1 (Non immunity 1) and unknown transcription factors.
- Published
- 2004
49. Regulation of the dual specificity protein phosphatase, DsPTP1, through interactions with calmodulin.
- Author
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Yoo JH, Cheong MS, Park CY, Moon BC, Kim MC, Kang YH, Park HC, Choi MS, Lee JH, Jung WY, Yoon HW, Chung WS, Lim CO, Lee SY, and Cho MJ
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Aniline Compounds chemistry, Arabidopsis, Binding Sites, Binding, Competitive, Blotting, Western, Calcium chemistry, Calcium metabolism, Calmodulin chemistry, DNA, Complementary metabolism, Dose-Response Relationship, Drug, Dual-Specificity Phosphatases, Gene Deletion, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Gene Library, Glutathione Transferase metabolism, Kinetics, Models, Genetic, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Organophosphorus Compounds chemistry, Peptides chemistry, Phosphoric Diester Hydrolases metabolism, Phosphorylation, Phosphotyrosine chemistry, Protein Binding, Protein Phosphatase 1, Protein Structure, Tertiary, Saccharomyces cerevisiae metabolism, Signal Transduction, Arabidopsis Proteins biosynthesis, Arabidopsis Proteins chemistry, Calmodulin metabolism, Protein Tyrosine Phosphatases biosynthesis, Protein Tyrosine Phosphatases chemistry, Protein Tyrosine Phosphatases genetics
- Abstract
Reversible phosphorylation is a key mechanism for the control of intercellular events in eukaryotic cells. In animal cells, Ca2+/CaM-dependent protein phosphorylation and dephosphorylation are implicated in the regulation of a number of cellular processes. However, little is known on the functions of Ca2+/CaM-dependent protein kinases and phosphatases in Ca2+ signaling in plants. From an Arabidopsis expression library, we isolated cDNA encoding a dual specificity protein phosphatase 1, which is capable of hydrolyzing both phosphoserine/threonine and phosphotyrosine residues of the substrates. Using a gel overlay assay, we identified two Ca2+-dependent CaM binding domains (CaMBDI in the N terminus and CaMBDII in the C terminus). Specific binding of CaM to two CaMBD was confirmed by site-directed mutagenesis, a gel mobility shift assay, and a competition assay using a Ca2+/CaM-dependent enzyme. At increasing concentrations of CaM, the biochemical activity of dual specificity protein phosphatase 1 on the p-nitrophenyl phosphate (pNPP) substrate was increased, whereas activity on the phosphotyrosine of myelin basic protein (MBP) was inhibited. Our results collectively indicate that calmodulin differentially regulates the activity of protein phosphatase, dependent on the substrate. Based on these findings, we propose that the Ca2+ signaling pathway is mediated by CaM cross-talks with a protein phosphorylation signal pathway in plants via protein dephosphorylation.
- Published
- 2004
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50. Mlo, a modulator of plant defense and cell death, is a novel calmodulin-binding protein. Isolation and characterization of a rice Mlo homologue.
- Author
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Kim MC, Lee SH, Kim JK, Chun HJ, Choi MS, Chung WS, Moon BC, Kang CH, Park CY, Yoo JH, Kang YH, Koo SC, Koo YD, Jung JC, Kim ST, Schulze-Lefert P, Lee SY, and Cho MJ
- Subjects
- Amino Acid Sequence, Base Sequence, Binding, Competitive, Blotting, Northern, Blotting, Southern, Calmodulin chemistry, Cell Membrane metabolism, Cloning, Molecular, Cytoplasm metabolism, DNA, Complementary metabolism, Escherichia coli metabolism, Gene Library, Genes, Plant, Molecular Sequence Data, Mutagenesis, Site-Directed, Peptides chemistry, Phosphoric Diester Hydrolases metabolism, Phylogeny, Protein Binding, Protein Isoforms, Protein Structure, Tertiary, Recombinant Proteins metabolism, Sequence Homology, Amino Acid, Signal Transduction, Subcellular Fractions metabolism, Time Factors, Calcium metabolism, Calmodulin metabolism, Oryza metabolism, Plant Proteins metabolism, Plant Proteins physiology
- Abstract
Transient influx of Ca(2+) constitutes an early event in the signaling cascades that trigger plant defense responses. However, the downstream components of defense-associated Ca(2+) signaling are largely unknown. Because Ca(2+) signals are mediated by Ca(2+)-binding proteins, including calmodulin (CaM), identification and characterization of CaM-binding proteins elicited by pathogens should provide insights into the mechanism by which Ca(2+) regulates defense responses. In this study, we isolated a gene encoding rice Mlo (Oryza sativa Mlo; OsMlo) using a protein-protein interaction-based screening of a cDNA expression library constructed from pathogen-elicited rice suspension cells. OsMlo has a molecular mass of 62 kDa and shares 65% sequence identity and scaffold topology with barley Mlo, a heptahelical transmembrane protein known to function as a negative regulator of broad spectrum disease resistance and leaf cell death. By using gel overlay assays, we showed that OsMlo produced in Escherichia coli binds to soybean CaM isoform-1 (SCaM-1) in a Ca(2+)-dependent manner. We located a 20-amino acid CaM-binding domain (CaMBD) in the OsMlo C-terminal cytoplasmic tail that is necessary and sufficient for Ca(2+)-dependent CaM complex formation. Specific binding of the conserved CaMBD to CaM was corroborated by site-directed mutagenesis, a gel mobility shift assay, and a competition assay with a Ca(2+)/CaM-dependent enzyme. Expression of OsMlo was strongly induced by a fungal pathogen and by plant defense signaling molecules. We propose that binding of Ca(2+)-loaded CaM to the C-terminal tail may be a common feature of Mlo proteins.
- Published
- 2002
- Full Text
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