39 results on '"Carvalho-Tavares, Juliana"'
Search Results
2. Cannabidiol Attenuates In Vivo Leukocyte Recruitment to the Spinal Cord Microvasculature at Peak Disease of Experimental Autoimmune Encephalomyelitis.
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Vitarelli da Silva, Thiago, Bernardes, Danielle, Oliveira-Lima, Onésia Cristina, Fernandes Pinto, Bárbara, Limborço Filho, Marcelo, Fraga Faraco, Camila Cristina, Juliano, Maria Aparecida, Esteves Arantes, Rosa Maria, A. Moreira, Fabrício, and Carvalho-Tavares, Juliana
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- 2024
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3. Mice chronically fed a high-fat diet are resistant to malaria induced by Plasmodium berghei ANKA
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Oliveira-Lima, Onésia Cristina, Almeida, Natália Lourenço, Almeida-Leite, Camila Megale, and Carvalho-Tavares, Juliana
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- 2019
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4. Detecting and tracking leukocytes in intravital video microscopy using a Hessian-based spatiotemporal approach
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Gregório da Silva, Bruno C., Carvalho-Tavares, Juliana, and Ferrari, Ricardo J.
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- 2019
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5. The synthetic peptide LyeTxI-b derived from Lycosa erythrognatha spider venom is cytotoxic to U-87 MG glioblastoma cells
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Abdel-Salam, Mostafa A. L., Carvalho-Tavares, Juliana, Gomes, Kamila Sousa, Teixeira-Carvalho, Andrea, Kitten, Gregory T., Nyffeler, Johanna, Dias, Felipe F., dos Reis, Pablo V. Mendes, Pimenta, Adriano M. C., Leist, Marcel, de Lima, Maria Elena, and de Souza-Fagundes, Elaine Maria
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- 2019
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6. Mas receptor deficiency exacerbates lipopolysaccharide-induced cerebral and systemic inflammation in mice
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Oliveira-Lima, Onésia C., Pinto, Mauro C.X., Duchene, Johan, Qadri, Fatimunnisa, Souza, Laura L., Alenina, Natalia, Bader, Michael, Santos, Robson A.S., and Carvalho-Tavares, Juliana
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- 2015
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7. Differential brain and spinal cord cytokine and BDNF levels in experimental autoimmune encephalomyelitis are modulated by prior and regular exercise
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Bernardes, Danielle, Oliveira-Lima, Onésia Cristina, da Silva, Thiago Vitarelli, Faraco, Camila Cristina Fraga, Leite, Hércules Ribeiro, Juliano, Maria Aparecida, dos Santos, Daniel Moreira, Bethea, John R., Brambilla, Roberta, Orian, Jacqueline M., Arantes, Rosa Maria Esteves, and Carvalho-Tavares, Juliana
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- 2013
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8. Mice lacking inducible nitric oxide synthase develop exacerbated hepatic inflammatory responses induced by Plasmodium berghei NK65 infection
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Oliveira-Lima, Onésia Cristina, Bernardes, Danielle, Xavier Pinto, Mauro Cunha, Esteves Arantes, Rosa Maria, and Carvalho-Tavares, Juliana
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- 2013
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9. The thrombolytic action of a proteolytic fraction (P1G10) from Carica candamarcensis
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Bilheiro, Rogério P., Braga, Ariadne D., Filho, Marcelo Limborço, Carvalho-Tavares, Juliana, Agero, Ubirajara, Carvalho, Maria das Graças, Sanchez, Eladio F., Salas, Carlos E., and Lopes, Miriam T.P.
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- 2013
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10. Nanomedicine internalization and penetration: why should we use spheroids?
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Pinto, Bárbara, Pacheco, Catarina, Silva, Patrícia, Carvalho-Tavares, Juliana, Sarmento, Bruno, and Bousbaa, Hassan
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Cancer is a leading cause of death worldwide and, despite medical advances in the development of new anticancer therapies, drug resistance, complicated side effects and poor efficacy remain a major public health problem. To breakdown the antisuppressive tumour strategies and to improve drug efficiency, nanomedicine is increasingly being used in anticancer therapy studies. Different in vitro experimental models and innovative approaches have been used in cancer research. In this aspect, 3D spheroids have become an attractive tool, overcoming many of the issues associated with using 2D models. Thus, in this review, an overview on 3D spheroids is provided, focusing on their use in the cancer field as a promising platform for nanomedicine studies. Thereafter, we highlight the main drug delivery systems currently developed and their applicability in 3D cell cultures., Scientific Letters, Vol. 1 No. 1 (2022)
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- 2022
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11. Automatic detection of motion blur in intravital video microscopy image sequences via directional statistics of log-Gabor energy maps
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Ferrari, Ricardo J., Villa Pinto, Carlos H., Gregório da Silva, Bruno C., Bernardes, Danielle, and Carvalho-Tavares, Juliana
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- 2015
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12. Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis
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Bernardes, Danielle, Brambilla, Roberta, Bracchi-Ricard, Valerie, Karmally, Shaffiat, Dellarole, Anna, Carvalho-Tavares, Juliana, and Bethea, John R
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- 2016
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13. Anti-inflammatory mechanisms and pharmacological actions of phycocyanobilin in a mouse model of experimental autoimmune encephalomyelitis: A therapeutic promise for multiple sclerosis.
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Marín-Prida, Javier, Pavón-Fuentes, Nancy, Lagumersindez-Denis, Nielsen, Camacho-Rodríguez, Hanlet, Margarita García-Soca, Ana, Sarduy-Chávez, Rocío de la Caridad, Marciano Vieira, Érica Leandro, Carvalho-Tavares, Juliana, Falcón-Cama, Viviana, Raúl Fernández-Massó, Julio, Hernández-González, Ignacio, Martínez-Donato, Gillian, Guillén-Nieto, Gerardo, Pentón-Arias, Eduardo, Martins Teixeira, Mauro, and Pentón-Rol, Giselle
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MULTIPLE sclerosis ,LABORATORY mice ,ENCEPHALOMYELITIS ,ANIMAL disease models ,NEUROLOGICAL disorders ,AUTOIMMUNE diseases ,MYELIN sheath diseases - Abstract
Cytokines, demyelination and neuroaxonal degeneration in the central nervous system are pivotal elements implicated in the pathogenesis of multiple sclerosis (MS) and its nonclinical model of experimental autoimmune encephalomyelitis (EAE). Phycocyanobilin (PCB), a chromophore of the biliprotein C-Phycocyanin (C-PC) from Spirulina platensis, has antioxidant, immunoregulatory and antiinflammatory effects in this disease, and it could complement the effect of other Disease Modifying Treatments (DMT), such as Interferon-b (IFN-b). Here, our main goal was to evaluate the potential PCB benefits and its mechanisms of action to counteract the chronic EAE in mice. MOG35-55-induced EAE was implemented in C57BL/6 female mice. Clinical signs, pro-inflammatory cytokines levels by ELISA, qPCR in the brain and immunohistochemistry using precursor/mature oligodendrocytes cells antibodies in the spinal cord, were assessed. PCB enhanced the neurological condition, and waned the brain concentrations of IL-17A and IL-6, pro-inflammatory cytokines, in a dosedependent manner. A down- or up-regulating activity of PCB at 1 mg/kg was identified in the brain on three (LINGO1, NOTCH1, and TNF-a), and five genes (MAL, CXCL12, MOG, OLIG1, and NKX2-2), respectively. Interestingly, a reduction of demyelination, active microglia/macrophages density, and axonal damage was detected along with an increase in oligodendrocyte precursor cells and mature oligodendrocytes, when assessed the spinal cords of EAE mice that took up PCB. The studies in vitro in rodent encephalitogenic T cells and in vivo in the EAE mouse model with the PCB/IFN-b combination, showed an enhanced positive effect of this combined therapy. Overall, these results demonstrate the anti-inflammatory activity and the protective properties of PCB on the myelin and support its use with IFN-b as an improved DMT combination for MS. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Navitoclax Enhances the Therapeutic Effects of PLK1 Targeting on Lung Cancer Cells in 2D and 3D Culture Systems.
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Pinto, Bárbara, Novais, Pedro, Henriques, Ana C., Carvalho-Tavares, Juliana, Silva, Patrícia M. A., and Bousbaa, Hassan
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CANCER cell culture ,LUNG cancer ,TREATMENT effectiveness ,CELL division ,CANCER cells ,CELL culture ,CELL death - Abstract
The efficacy of antimitotics is limited by slippage, whereby treated cells arrested in mitosis exit mitosis without cell division and, eventually, escape apoptosis, constituting a serious resistance mechanism to antimitotics. Strategies to overcome slippage should potentiate the cancer cell killing activity of these antimitotics. Such strategies should accelerate cell death in mitosis before slippage. Here, we undertook a mechanistic analysis to test whether the apoptosis activator Navitoclax potentiates apoptosis triggered by the antimitotic BI2536, a potent inhibitor of Polo-like kinase 1 (PLK1) with the goal of overcoming slippage. We found that cancer cells in 2D cultures treated with BI2536 alone accumulate in mitosis, but a significant fraction of arrested cells undergo slippage and survive. Remarkably, combining BI2536 with Navitoclax dramatically reduces slippage, shifting the cell fate to accelerated death in mitosis. The results are confirmed in 3D spheroids, a preclinical system that mimics in vivo tumor drug responses. Importantly, in 3D spheroids, the effect of the BI2536/Navitoclax combination requires a lower therapeutic dosage of each drug, underlying its potential to improve the therapeutic index. Our results highlight the relevance of apoptosis potentiators to circumvent slippage associated with antimitotics. The combination of BI2536 with Navitoclax shows in vitro synergy/additive effect, which warrants further clinical research. [ABSTRACT FROM AUTHOR]
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- 2022
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15. Effect of mutalysin II on vascular recanalization after thrombosis induction in the ear of the hairless mice model
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Agero, Ubirajara, Arantes, Rosa M.E., Lacerda-Queiroz, Norinne, Mesquita, Oscar N., Magalhães, A., Sanchez, Eladio F., and Carvalho-Tavares, Juliana
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- 2007
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16. Swim training attenuates oxidative damage and promotes neuroprotection in cerebral cortical slices submitted to oxygen glucose deprivation
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Leite, Hércules R., Mourão, Flávio A. G., Drumond, Luciana E., Ferreira-Vieira, Talita H., Bernardes, Danielle, Silva, Josiane F., Lemos, Virgínia S., Moraes, Márcio F. D., Pereira, Grace S., Carvalho-Tavares, Juliana, and Massensini, André R.
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- 2012
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17. Beneficial actions of oleanolic acid in an experimental model of multiple sclerosis: A potential therapeutic role
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Martín, Rubén, Carvalho-Tavares, Juliana, Hernández, Marita, Arnés, Mercedes, Ruiz-Gutiérrez, Valentina, and Nieto, María Luisa
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- 2010
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18. CCL2 and CCL5 mediate leukocyte adhesion in experimental autoimmune encephalomyelitis—an intravital microscopy study
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dos Santos, Adriana Carvalho, Barsante, Michele Mendes, Esteves Arantes, Rosa Maria, Bernard, Claude C.A., Teixeira, Mauro Martins, and Carvalho-Tavares, Juliana
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- 2005
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19. Inhalation of dimethyl fumarate-encapsulated solid lipid nanoparticles attenuate clinical signs of experimental autoimmune encephalomyelitis and pulmonary inflammatory dysfunction in mice.
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Fernandes Pinto, Bárbara, Brito Ribeiro, Lorena Natasha, Rolfsen Ferreira da Silva, Gisela Bevilacqua, Simões Freitas, Camila, Kraemer, Lucas, Silva Oliveira, Fabrício Marcus, Carvalho Clímaco, Marianna, Gonçalves Mourão, Flávio Afonso, Pinheiro dos Santos, Gabryella Soares, Ribeiro Béla, Samantha, da Silva Gurgel, Isabella Luísa, de Lima Leite, Fábio, Gomes de Oliveira, Anselmo, Silva da Páscoa Vilela, Maura Regina, Cristina Oliveira-Lima, Onésia, Soriani, Frederico Marianetti, Fujiwara, Ricardo Toshio, Birbrair, Alexander, Castro Russo, Remo, and Carvalho-Tavares, Juliana
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ENCEPHALITIS ,SYMPTOMS ,MYELITIS ,ENCEPHALOMYELITIS ,DIMETHYL fumarate ,GASTROINTESTINAL system - Abstract
Rationale: The FDA-approved Dimethyl Fumarate (DMF) as an oral drug for Multiple Sclerosis (MS) treatment based on its immunomodulatory activities. However, it also caused severe adverse effects mainly related to the gastrointestinal system. Objective: Investigated the potential effects of solid lipid nanoparticles (SLNs) containing DMF, administered by inhalation on the clinical signs, central nervous system (CNS) inflammatory response, and lung function changes in mice with experimental autoimmune encephalomyelitis (EAE). Materials and methods: EAE was induced using MOG
35-55 peptide in female C57BL/6J mice and the mice were treated via inhalation with DMF-encapsulated SLN (CTRL/SLN/DMF and EAE/SLN/DMF), empty SLN (CTRL/SLN and EAE/SLN), or saline solution (CTRL/saline and EAE/saline), every 72 h during 21 days. Results: After 21 days post-induction, EAE mice treated with DMF-loaded SLN, when compared with EAE/saline and EAE/SLN, showed decreased clinical score and weight loss, reduction in brain and spinal cord injury and inflammation, also related to the increased influx of Foxp3+ cells into the spinal cord and lung tissues. Moreover, our data revealed that EAE mice showed signs of respiratory disease, marked by increased vascular permeability, leukocyte influx, production of TNF-α and IL-17, perivascular and peribronchial inflammation, with pulmonary mechanical dysfunction associated with loss of respiratory volumes and elasticity, which DMF-encapsulated reverted in SLN nebulization. Conclusion: Our study suggests that inhalation of DMF-encapsulated SLN is an effective therapeutic protocol that reduces not only the CNS inflammatory process and disability progression, characteristic of EAE disease, but also protects mice from lung inflammation and pulmonary dysfunction. [ABSTRACT FROM AUTHOR]- Published
- 2022
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20. Kinin B2 receptor regulates chemokines CCL2 and CCL5 expression and modulates leukocyte recruitment and pathology in experimental autoimmune encephalomyelitis (EAE) in mice
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Bader Michael, Pesquero João B, Pesquero Jorge L, Juliano Luiz, Arantes Rosa ME, Roffê Ester, Dos Santos Adriana C, Teixeira Mauro M, and Carvalho-Tavares Juliana
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Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Kinins are important mediators of inflammation and act through stimulation of two receptor subtypes, B1 and B2. Leukocyte infiltration contributes to the pathogenesis of autoimmune inflammation in the central nervous system (CNS), occurring not only in multiple sclerosis (MS) but also in experimental autoimmune encephalomyelitis (EAE). We have previously shown that the chemokines CCL2 and CCL5 play an important role in the adhesion of leukocytes to the brain microcirculation in EAE. The aim of the present study was to evaluate the relevance of B2 receptors to leukocyte-endothelium interactions in the cerebral microcirculation, and its participation in CNS inflammation in the experimental model of myelin-oligodendrocyte-glycoprotein (MOG)35–55-induced EAE in mice. Methods In order to evaluate the role of B2 receptor in the cerebral microvasculature we used wild-type (WT) and kinin B2 receptor knockout (B2-/-) mice subjected to MOG35–55-induced EAE. Intravital microscopy was used to investigate leukocyte recruitment on pial matter vessels in B2-/- and WT EAE mice. Histological documentation of inflammatory infiltrates in brain and spinal cords was correlated with intravital findings. The expression of CCL5 and CCL2 in cerebral tissue was assessed by ELISA. Results Clinical parameters of disease were reduced in B2-/- mice in comparison to wild type EAE mice. At day 14 after EAE induction, there was a significant decrease in the number of adherent leukocytes, a reduction of cerebral CCL5 and CCL2 expressions, and smaller inflammatory and degenerative changes in B2-/- mice when compared to WT. Conclusion Our results suggest that B2 receptors have two major effects in the control of EAE severity: (i) B2 regulates the expression of chemokines, including CCL2 and CCL5, and (ii) B2 modulates leukocyte recruitment and inflammatory lesions in the CNS.
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- 2008
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21. Pericytes modulate myelination in the central nervous system.
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Azevedo, Patrick O., Sena, Isadora F. G., Andreotti, Julia P., Carvalho‐Tavares, Juliana, Alves‐Filho, José C., Cunha, Thiago M., Cunha, Fernando Q., Mintz, Akiva, and Birbrair, Alexander
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PERICYTES ,MYELINATION ,MULTIPLE sclerosis ,DISEASE prevalence ,NERVOUS system regeneration - Abstract
Multiple sclerosis is a highly prevalent chronic demyelinating disease of the central nervous system. Remyelination is the major therapeutic goal for this disorder. The lack of detailed knowledge about the cellular and molecular mechanisms involved in myelination restricts the design of effective treatments. A recent study by using [De La Fuente et al. (2017) Cell Reports, 20(8): 1755‐1764] by using state‐of‐the‐art techniques, including pericyte‐deficient mice in combination with induced demyelination, reveal that pericytes participate in central nervous system regeneration. Strikingly, pericytes presence is essential for oligodendrocyte progenitors differentiation and myelin formation during remyelination in the brain. The emerging knowledge from this research will be important for the treatment of multiple sclerosis. [ABSTRACT FROM AUTHOR]
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- 2018
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22. Use of a pentacyclic triterpene in a pharmaceutical composition for the treatment of multiple sclerosis
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Nieto, María Luisa, Martín Montana, Rubén, Carvalho-Tavares, Juliana, and Ruiz-Gutiérrez, Valentina
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Oleanolic acid (3[beta]-hiydroxyolean-12-en-28-oic acid) is a pentacyclic triterpene occurring in a large number of medicinal plants. The present invention is directed to the pharmacological application of the oleanolic acid, alone or in combination with other substances, in the treatment or prophylaxis of neurodegenerative diseases such as multiple sclerosis. The inventors have found that oleanolic acid, intraperitoneally administered once daily, reduces significantly the immuno-inflammatory and neurological symptoms associated with the experimental autoimmune encephalomyelitis, an experimental model of multiple sclerosis, delaying the onset and reducing the progression of the disease., Consejo Superior de Investigaciones Científicas (España), A1 Solicitud de patente con informe sobre el estado de la técnica
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- 2009
23. Oleanolsäure zur Behandlung von Multipler Sklerose
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Nieto, María Luisa, Martín, Rubén, Carvalho-Tavares, Juliana, and Ruiz-Gutiérrez, Valentina
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[EN] The invention relates to the use of pentacyclic triterpene for the preparation of a pharmaceutical compound intended for the treatment of multiple sclerosis. Oleanolic acid (3ss-hydroxyolean-12-en-28-oic acid) is a pentacyclic triterpene acid present in a large number of medicinal plants. The invention relates to the pharmacological use of oleanolic acid either alone or combined with other substances in the therapy or prophylaxis of neurodegenerative diseases, such as multiple sclerosis. The applicants have found that the daily intraperitoneal administration of oleanolic acid significantly reduces the neurological and immunoinflammatory symptoms associated with experimental autoimmune encephalomyelitis, an experimental model of multiple sclerosis, delaying the onset and reducing the progression of the disease., [ES] La invención relaciona al uso del triterpene pentacyclic para la preparación de un compuesto farmacéutico destinado para el tratamiento de la esclerosis múltiple. El ácido de Oleanolic (ácido 3ss-hydroxyolean-12-en-28-oic) es un ácido pentacyclic del triterpene presente en una gran cantidad de plantas medicinales. La invención relaciona al uso farmacológico del ácido oleanolic solo o combinado con las distintas sustancias en la terapia o la profilaxis de enfermedades neurodegenerativas, tales como esclerosis múltiple. Los solicitantes han encontrado que la administración intraperitoneal diaria del ácido oleanolic reduce perceptiblemente los síntomas neurológicos e immunoinflammatory asociados con encephalomyelitis autoinmune experimental, un modelo experimental de la esclerosis múltiple, delaying el comienzo y reducir la progresión de la enfermedad., Consejo Superior de Investigaciones Científicas (España), B1 Patente sin examen previo
- Published
- 2009
24. Is Peripheral Immunity Regulated by Blood-Brain Barrier Permeability Changes?
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Bargerstock, Erin, Puvenna, Vikram, Iffland, Philip, Falcone, Tatiana, Hossain, Mohammad, Vetter, Stephen, Man, Shumei, Dickstein, Leah, Marchi, Nicola, Ghosh, Chaitali, Carvalho-Tavares, Juliana, and Janigro, Damir
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BLOOD-brain barrier ,PERMEABILITY (Biology) ,AUTOANTIBODIES ,ANTIGEN presenting cells ,EPILEPSY ,IMMUNOREGULATION ,IMMUNOHISTOCHEMISTRY - Abstract
S100B is a reporter of blood-brain barrier (BBB) integrity which appears in blood when the BBB is breached. Circulating S100B derives from either extracranial sources or release into circulation by normal fluctuations in BBB integrity or pathologic BBB disruption (BBBD). Elevated S100B matches the clinical presence of indices of BBBD (gadolinium enhancement or albumin coefficient). After repeated sub-concussive episodes, serum S100B triggers an antigen-driven production of anti-S100B autoantibodies. We tested the hypothesis that the presence of S100B in extracranial tissue is due to peripheral cellular uptake of serum S100B by antigen presenting cells, which may induce the production of auto antibodies against S100B. To test this hypothesis, we used animal models of seizures, enrolled patients undergoing repeated BBBD, and collected serum samples from epileptic patients. We employed a broad array of techniques, including immunohistochemistry, RNA analysis, tracer injection and serum analysis. mRNA for S100B was segregated to barrier organs (testis, kidney and brain) but S100B protein was detected in immunocompetent cells in spleen, thymus and lymph nodes, in resident immune cells (Langerhans, satellite cells in heart muscle, etc.) and BBB endothelium. Uptake of labeled S100B by rat spleen CD4+ or CD8+ and CD86+ dendritic cells was exacerbated by pilocarpine-induced status epilepticus which is accompanied by BBBD. Clinical seizures were preceded by a surge of serum S100B. In patients undergoing repeated therapeutic BBBD, an autoimmune response against S100B was measured. In addition to its role in the central nervous system and its diagnostic value as a BBBD reporter, S100B may integrate blood-brain barrier disruption to the control of systemic immunity by a mechanism involving the activation of immune cells. We propose a scenario where extravasated S100B may trigger a pathologic autoimmune reaction linking systemic and CNS immune responses. [ABSTRACT FROM AUTHOR]
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- 2014
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25. Intracellular and circulating neuronal antinuclear antibodies in human epilepsy.
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Iffland, Philip H., Carvalho-Tavares, Juliana, Trigunaite, Abhishek, Man, Shumei, Rasmussen, Peter, Alexopoulos, Andreas, Ghosh, Chaitali, Jørgensen, Trine N., and Janigro, Damir
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INTRACELLULAR membranes , *ANTINUCLEAR factors , *TREATMENT of epilepsy , *AUTOIMMUNITY , *AUTOANTIBODIES , *POTASSIUM channels , *COMPARATIVE studies - Abstract
Abstract: There are overwhelming data supporting the inflammatory origin of some epilepsies (e.g., Rasmussen's encephalitis and limbic encephalitis). Inflammatory epilepsies with an autoimmune component are characterized by autoantibodies against membrane-bound, intracellular or secreted proteins (e.g., voltage gated potassium channels). Comparably, little is known regarding autoantibodies targeting nuclear antigen. We tested the hypothesis that in addition to known epilepsy-related autoantigens, the human brain tissue and serum from patients with epilepsy contain autoantibodies recognizing nuclear targets. We also determined the specific nuclear proteins acting as autoantigen in patients with epilepsy. Brain tissue samples were obtained from patients undergoing brain resections to treat refractory seizures, from the brain with arteriovenous malformations or from post-mortem multiple sclerosis brain. Patients with epilepsy had no known history of autoimmune disease and were not diagnosed with autoimmune epilepsy. Tissue was processed for immunohistochemical staining. We also obtained subcellular fractions to extract intracellular IgGs. After separating nuclear antibody–antigen complexes, the purified autoantigen was analyzed by mass spectrometry. Western blots using autoantigen or total histones were probed to detect the presence of antinuclear antibodies in the serum of patients with epilepsy. Additionally, HEp-2 assays and antinuclear antibody ELISA were used to detect the staining pattern and specific presence of antinuclear antibodies in the serum of patients with epilepsy. Brain regions from patients with epilepsy characterized by blood–brain barrier disruption (visualized by extravasated albumin) contained extravasated IgGs. Intracellular antibodies were found in epilepsy (n=13/13) but not in multiple sclerosis brain (n=4/4). In the brain from patients with epilepsy, neurons displayed higher levels of nuclear IgGs compared to glia. IgG colocalized with extravasated albumin. All subcellular fractions from brain resections of patients with epilepsy contained extravasated IgGs (n=10/10), but epileptogenic cortex, where seizures originated from, displayed the highest levels of chromatin-bound IgGs. In the nuclear IgG pool, anti-histone autoantibodies were identified by two independent immunodetection methods. HEp-2 assay and ELISA confirmed the presence of anti-histone (n=5/8) and anti-chromatin antibodies in the serum from patients with epilepsy. We developed a multi-step approach to unmask autoantigens in the brain and sera of patients with epilepsy. This approach revealed antigen-bound antinuclear antibodies in neurons and free antinuclear IgGs in the serum of patients with epilepsy. Conditions with blood–brain barrier disruption but not seizures, were characterized by extravasated but not chromatin-bound IgGs. Our results show that the pool of intracellular IgG in the brain of patients with epilepsy consists of nucleus-specific autoantibodies targeting chromatin and histones. Seizures may be the trigger of neuronal uptake of antinuclear antibodies. [Copyright &y& Elsevier]
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- 2013
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26. Natural Triterpenic Diols Promote Apoptosis in Astrocytoma Cells through ROS-Mediated Mitochondrial Depolarization and JNK Activation.
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Martín, Rubén, Ibeas, Elvira, Carvalho-Tavares, Juliana, Hernández, Marita, Ruiz-Gutierrez, Valentina, and Nieto, María Luisa
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APOPTOSIS ,ASTROCYTOMAS ,MITOCHONDRIA ,CELL proliferation ,ANTINEOPLASTIC agents ,CELL death - Abstract
Background: Triterpene alcohols and acids are multifunctional compounds widely distributed throughout the plant kingdom that exhibit a variety of beneficial health properties, being synthetic analogs of oleanolic acid under clinical evaluation as anti-tumoral therapeutic agents. However, the antineoplastic activity of two natural occuring triterpenoid alcohols extracted from olive oil, erythrodiol (an intermediate from oleanolic acid), and its isomer, uvaol, has barely been reported, particularly on brain cancer cells. Astrocytomas are among the most common and aggressive type of primary malignant tumors in the neurological system lacking effective treatments, and in this study, we addressed the effect of these two triterpenic diols on the human 1321N1 astrocytoma cell line. Principal Findings: Erythrodiol and uvaol effectively affected cell proliferation, as well as cell cycle phases and induced 1321N1 cell death. Both triterpenes successfully modulated the apoptotic response, promoting nuclear condensation and fragmentation. They caused retraction and rounding of cultured cells, which lost adherence from their supports, while F-actin and vimentin filaments disappeared as an organized cytoplasmic network. At molecular level, changes in the expression of surface proteins associated with adhesion or death processes were also observed. Moreover, triterpene exposure resulted in the production of reactive oxygen species (ROS) with loss of mitochondrial transmembrane potential, and correlated with the activation of c-Jun N-terminal kinases (JNK). The presence of catalase reversed the triterpenic diolsinduced mitochondrial depolarization, JNK activation, and apoptotic death, indicating the critical role of ROS in the action of these compounds. Conclusions: Overall, we provide a significant insight into the anticarcinogenic action of erythrodiol and uvaol that may have a potential in prevention and treatment of brain tumors and other cancers. [ABSTRACT FROM AUTHOR]
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- 2009
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27. Salivation pattern of Rhodnius prolixus (Reduviidae; Triatominae) in mouse skin
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Soares, Adriana Coelho, Carvalho-Tavares, Juliana, Gontijo, Nelder de Figueiredo, dos Santos, Vânia Cristina, Teixeira, Mauro Martins, and Pereira, Marcos Horácio
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RHODNIUS prolixus , *INSECT feeding & feeds , *ACRIDINE , *TRIATOMA , *SALIVARY glands , *IMMUNE response - Abstract
Abstract: The objective of this work was to study the pattern of salivation of triatomines during feeding in mouse skin. Rhodnius prolixus was fed with a solution of the dye acridine orange or fluorescein. The saliva was efficiently labelled with acridine orange, probably due to the difference in pH between the salivary gland (⩽6.0) and the hemolymph (6.5–7.0). This procedure was not effective at labelling the saliva of Triatoma infestans, however, fluorescent labelling of R. prolixus saliva allowed us to demonstrate that salivation occurs during entire feeding process. The saliva is released soon after the bite. In the probing phase, saliva is pumped continuously in the host skin, including around the blood vessels. During the engorgement phase, saliva is observed in a bolus within the blood vessel and some of it is sucked up by the insect, together with blood. The frequency of saliva emission inside the vessels was low (0.51±0.18Hz). The saliva deposition in the microcirculation is continuous and modulated by the frequency of the cibarial pump because, when functioning at high frequency, cibarial pump sucks almost all saliva to the insect gut. This mechanism would determine the quantity of saliva deposited in the microcirculation as necessary, and consequently minimizing the host''s immune response to salivary antigens. [Copyright &y& Elsevier]
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- 2006
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28. A Role for Platelets and Endothelial Selectins in Tumor Necrosis Factor-α-Induced Leukocyte Recruitment in the Brain Microvasculature.
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Carvalho-Tavares, Juliana, Hickey, Michael J., Hutchison, Jamie, Michaud, Jean, Sutcliffe, Ian T., and Kubes, Paul
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- 2000
29. Translational inhibition of E-selectin expression stimulates P-selectin-dependent neutrophil recruitment.
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Ostrovsky, Lena and Carvalho-Tavares, Juliana
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THROMBIN , *NEUTROPHILS , *ENDOTHELIUM , *PHYSIOLOGY - Abstract
Presents a study which examined the thrombin-induced neutrophil interactions with human endothelium. Methodology; Results and discussion; Conclusions.
- Published
- 2000
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30. LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model.
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Abdel-Salam, Mostafa A. L., Pinto, Bárbara, Cassali, Geovanni, Bueno, Lilian, Pêgas, Gabriela, Oliveira, Fabrício, Silva, Irismara, Klein, André, Souza-Fagundes, Elaine Maria de, de Lima, Maria Elena, and Carvalho-Tavares, Juliana
- Subjects
METASTASIS ,SPIDER venom ,BREAST cancer ,MICE ,TUMOR growth ,LUNG tumors ,SNAKEBITES - Abstract
Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model. Mice with a palpable tumor in the left flank were subcutaneously or intratumorally injected with LyeTx I-b (5 mg/kg), which significantly decreased the tumor volume and metastatic nodules. Histological analyses showed a large necrotic area in treated primary tumors compared to the control. LyeTxI-b reduced tumor growth and lung metastasis in the 4T1 mouse mammary carcinoma model with no signs of toxicity in healthy or cancerous mice. The mechanism of action of LyeTx I-b on the 4T1 mouse mammary carcinoma model was evaluated in vitro and is associated with induction of apoptosis and cell proliferation inhibition. Furthermore, LyeTx I-b seems to be an efficient regulator of the 4T1 tumor microenvironment by modulating several cytokines, such as TGF-β, TNF-α, IL-1β, IL-6, and IL-10, in primary tumor and lung, spleen, and brain. LyeTx I-b also plays a role in leukocytes rolling and adhesion into spinal cord microcirculation and in the number of circulating leukocytes. These data suggest a potent antineoplastic efficacy ofLyeTx I-b. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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31. Assessment of the mechanism of juxtacrine activation and adhesion of leukocytes in liver...
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Carvalho-Tavares, Juliana and Fox-Robichaud, Alison
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- *
CELL adhesion , *LEUCOCYTES , *LIVER , *CYTOLOGY - Abstract
Studies the mechanism of juxtacrine activation and adhesion of leukocytes in liver microcirculation. Effect of leukotriene C4 (LTC4) on leukocyte recruitment in liver microvasculature; Leukocyte recruitment in response to histamine superfusion with or without an antihistamine; Activity of stationary leukocytes within sinusoids.
- Published
- 1999
32. Cover Image, Volume 233, Number 8, August 2018.
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Azevedo, Patrick O., Sena, Isadora F. G., Andreotti, Julia P., Carvalho‐Tavares, Juliana, Alves‐Filho, José C., Cunha, Thiago M., Cunha, Fernando Q., Mintz, Akiva, and Birbrair, Alexander
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PERICYTES ,MYELINATION - Published
- 2018
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33. Intravital microscopy and image analysis of Rhodnius prolixus (Hemiptera: Reduviidae) hematophagy: The challenge of blood intake from mouse skin.
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Soares, Adriana Coelho, Araújo, Ricardo Nascimento, Carvalho-Tavares, Juliana, Gontijo, Nelder de Figueiredo, and Pereira, Marcos Horácio
- Subjects
- *
RHODNIUS prolixus , *BLOODSUCKING insects , *TRANSMISSION of parasitic diseases , *LABORATORY mice , *SKIN physiology , *BLOOD meal as feed - Abstract
Abstract: Hematophagous insects transmit many of the most dangerous parasitic diseases. The transmission usually occurs during hematophagy or just after as this is when the vector and the host are in contact. The contact time is determined by the feeding performance of the insect in each host. In triatomines, feeding performance interferes with both their life cycle and the vectorial competence to transmit the hemoflagellate Trypanosoma cruzi. Triatomine bugs are vessel feeders, obtaining their blood meals directly from the vessels (venules or arterioles) of their vertebrate hosts. The host blood intake rate is not constant during the feeding, and the sucking frequency of triatomines tends to be higher and to contain fewer interruptions in pigeons than in mice. To identify the difficulties encountered by triatomine bugs in obtaining blood meals from mouse skin, we used intravital microscopy techniques associated with electromyograms of the cibarial pump. To monitor the vibration of the cannulated vessels and the blood flow through the head of the insect during the engorgement phase, we introduced a novel method for image analysis. The mean number of vessels used during a Rhodnius prolixus blood meal was 3.4±1.2, and the insects fed more in venules (63%) than in arterioles (37%). An important increase in vascular permeability was observed throughout the feeding. Platelet aggregation, rolling and leukocyte adherence were analyzed on the venular endothelium, showing remarkable increases for some time following the R. prolixus feeding. The reduction in sucking frequency that was observed during insect feeding was likely due to the increased cibarial pump filling time. The monitoring of the vessel wall pulsation also permitted the registration of regurgitation-like movements during blood pumping, with these movements being recorded mostly during the second half of the feeding. The evaluation of blood flow through the head of the insect suggested that the regurgitation-like movements were not true regurgitations and were caused by abrupt difficulties in the function of the cibarial pump. The role of the platelet plugs and the changes in blood viscosity at the R. prolixus feeding site are discussed. The method introduced in the present study to analyze the images brings new insights into the interaction between hematophagous vectors and their hosts, reinforcing the importance of insect saliva throughout the feeding process. [Copyright &y& Elsevier]
- Published
- 2014
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- View/download PDF
34. Blockade of the renin–angiotensin system improves cerebral microcirculatory perfusion in diabetic hypertensive rats.
- Author
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Estato, Vanessa, Obadia, Nathalie, Carvalho-Tavares, Juliana, Freitas, Felipe Santos, Reis, Patrícia, Neto, Hugo Castro-Faria, Lessa, Marcos Adriano, and Tibiriçá, Eduardo
- Subjects
- *
RENIN-angiotensin system , *CEREBRAL circulation , *DIABETIC nephropathies , *MICROCIRCULATION , *BRAIN blood-vessels , *LABORATORY rats , *ACE inhibitors , *BRAIN function localization - Abstract
Abstract: We examined the functional and structural microcirculatory alterations in the brain, skeletal muscle and myocardium of non-diabetic spontaneously hypertensive rats (SHR) and diabetic SHR (D-SHR), as well as the effects of long-term treatment with the angiotensin AT1-receptor antagonist olmesartan and the angiotensin-converting enzyme inhibitor enalapril. Diabetes was experimentally induced by a combination of a high-fat diet with a single low dose of streptozotocin (35mg/kg, intraperitoneal injection). D-SHR were orally administered with olmesartan (5mg/kg/day), enalapril (10mg/kg/day) or vehicle for 28days, and compared with vehicle-treated non-diabetic SHR or normotensive non-diabetic Wistar–Kyoto rats. The cerebral and skeletal muscle functional capillary density of pentobarbital-anesthetized rats was assessed using intravital fluorescence videomicroscopy. Chronic treatment with olmesartan or enalapril significantly lowered blood pressure and reversed brain functional capillary rarefaction. Brain oxidative stress was reduced to non-diabetic control levels in animals treated with olmesartan or enalapril. Histochemical analysis of the structural capillary density showed that both olmesartan and enalapril increased the capillary-to-fiber ratio in skeletal muscle and the capillary-to-fiber volume density in the left ventricle. Olmesartan and enalapril also prevented collagen deposition and the increase in cardiomyocyte diameter in the left ventricle. Our results suggest that the association between hypertension and diabetes results in microvascular alterations in the brain, skeletal muscle and myocardium that can be prevented by chronic blockade of the renin–angiotensin system. [Copyright &y& Elsevier]
- Published
- 2013
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35. Effects of tityustoxin on cerebral inflammatory response in young rats.
- Author
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Van Fraga, Iva Tereza, Limborço-Filho, Marcelo, Lima, Onésia Cristina Oliveira, Lacerda-Queiroz, Norinne, Guidine, Patrícia Alves Maia, Moraes, Márcio Flávio Dutra, Nascimento Araújo, Ricardo, Moraes-Santos, Tasso, Massensini, André Ricardo, Arantes, Rosa Maria Esteves, and Carvalho-Tavares, Juliana
- Subjects
- *
ION channels , *CEREBRAL arteries , *LABORATORY rats , *TITYUS , *VENOM , *NEUROIMMUNOLOGY , *WOUNDS & injuries - Abstract
Accidents caused by scorpion stings, mainly affecting children, are considered an important cause of morbidity and mortality in tropical countries. Clinical studies demonstrate the relevant role of systemic inflammatory events in scorpion envenoming. However, remains poorly understood whether the major lethal component in Tityus serrulatus venom, tityustoxin (TsTX), is able to induce inflammatory responses in the cerebral microcirculation. In this study, we systematically examined leukocyte recruitment into the CNS in response to TsTX injection. Accordingly, developing rats were subjected to a subcutaneous (s.c.) injection of TsTX (0.75 mg/kg), and leukocyte recruitment (i.e., 4, 8 and 12 h after injection) and TNF-α levels were evaluated. Rats injected with TsTX presented a significant increase in leukocyte rolling and adhesion and higher levels of TNF-α at all time points studied, compared to the control group. Altogether, this work demonstrates the triggering of neuroimmunological mechanisms induced by TsTX injection in young rats. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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36. Plasmodium berghei NK65 induces cerebral leukocyte recruitment in vivo: An intravital microscopic study
- Author
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Lacerda-Queiroz, Norinne, Lima, Onésia Cristina Oliveira, Carneiro, Cláudia Martins, Vilela, Márcia Carvalho, Teixeira, Antônio Lúcio, Carvalho, Andrea Teixeira-, Araújo, Márcio Sobreira Silva, Martins-Filho, Olindo Assis, Braga, Érika Martins, and Carvalho-Tavares, Juliana
- Subjects
- *
PLASMODIUM , *LEUCOCYTES , *MALARIA , *MICROCIRCULATION , *CHEMOKINES , *ENCEPHALITIS , *HISTOPATHOLOGY , *IMMUNE response - Abstract
Abstract: Malaria is second only to tuberculosis as the leading cause of morbidity and mortality as a consequence of a single infectious agent. Much of the pathology of malaria arises from the inappropriate or excessive immune response mounted by the host in an attempt to eliminate the parasite. We here report the inflammatory changes observed in the cerebral microvasculature of C57BL/6 and BALB/c mice that had been inoculated with Plasmodium berghei NK65, a lethal strain of rodent malaria. Although no neurological signs were observed in experimentally infected mice, inflammation of the cerebral microvasculature was clearly evident. Histopathological analysis demonstrated that alterations in cerebral tissue were more intense in infected C57Bl/6 mice than in infected BALB/c animals. Intravital microscopic examination of the cerebral microvasculature revealed increased leukocyte rolling and adhesion in pial venules of infected mice compared with non-infected animals. The extravasation of Evans blue dye into the cerebral parenchyma was also elevated in infected mice in comparison with their non-infected counterparts. Additionally, protein levels of TNF-α, MIG/CXCL9, MCP-1/CCL2, MIP-1α/CCL3 and RANTES/CCL5 were up-regulated in brain samples derived from infected C57Bl/6 mice. Taken together, the data reported here illustrate the complex strain-dependent relationships between leukocyte recruitment, blood brain barrier permeability and chemokine production. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
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37. Inhalation of dimethyl fumarate-encapsulated solid lipid nanoparticles attenuate clinical signs of experimental autoimmune encephalomyelitis and pulmonary inflammatory dysfunction in mice.
- Author
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Pinto BF, Ribeiro LNB, da Silva GBRF, Freitas CS, Kraemer L, Oliveira FMS, Clímaco MC, Mourão FAG, Santos GSPD, Béla SR, Gurgel ILDS, Leite FL, de Oliveira AG, Vilela MRSDP, Oliveira-Lima OC, Soriani FM, Fujiwara RT, Birbrair A, Russo RC, and Carvalho-Tavares J
- Subjects
- Administration, Inhalation, Animals, Disease Models, Animal, Female, Immunosuppressive Agents administration & dosage, Mice, Inbred C57BL, Multiple Sclerosis, Mice, Dimethyl Fumarate administration & dosage, Encephalomyelitis, Autoimmune, Experimental drug therapy, Liposomes administration & dosage, Nanoparticles administration & dosage, Pneumonia drug therapy
- Abstract
Rationale: The FDA-approved Dimethyl Fumarate (DMF) as an oral drug for Multiple Sclerosis (MS) treatment based on its immunomodulatory activities. However, it also caused severe adverse effects mainly related to the gastrointestinal system., Objective: Investigated the potential effects of solid lipid nanoparticles (SLNs) containing DMF, administered by inhalation on the clinical signs, central nervous system (CNS) inflammatory response, and lung function changes in mice with experimental autoimmune encephalomyelitis (EAE)., Materials and Methods: EAE was induced using MOG35-55 peptide in female C57BL/6J mice and the mice were treated via inhalation with DMF-encapsulated SLN (CTRL/SLN/DMF and EAE/SLN/DMF), empty SLN (CTRL/SLN and EAE/SLN), or saline solution (CTRL/saline and EAE/saline), every 72 h during 21 days., Results: After 21 days post-induction, EAE mice treated with DMF-loaded SLN, when compared with EAE/saline and EAE/SLN, showed decreased clinical score and weight loss, reduction in brain and spinal cord injury and inflammation, also related to the increased influx of Foxp3+ cells into the spinal cord and lung tissues. Moreover, our data revealed that EAE mice showed signs of respiratory disease, marked by increased vascular permeability, leukocyte influx, production of TNF-α and IL-17, perivascular and peribronchial inflammation, with pulmonary mechanical dysfunction associated with loss of respiratory volumes and elasticity, which DMF-encapsulated reverted in SLN nebulization., Conclusion: Our study suggests that inhalation of DMF-encapsulated SLN is an effective therapeutic protocol that reduces not only the CNS inflammatory process and disability progression, characteristic of EAE disease, but also protects mice from lung inflammation and pulmonary dysfunction., (© 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
- Published
- 2022
- Full Text
- View/download PDF
38. Kinin B2 receptor regulates chemokines CCL2 and CCL5 expression and modulates leukocyte recruitment and pathology in experimental autoimmune encephalomyelitis (EAE) in mice.
- Author
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Dos Santos AC, Roffê E, Arantes RM, Juliano L, Pesquero JL, Pesquero JB, Bader M, Teixeira MM, and Carvalho-Tavares J
- Subjects
- Animals, Brain blood supply, Brain metabolism, Brain pathology, Chemokines metabolism, Cytokines metabolism, Encephalomyelitis, Autoimmune, Experimental pathology, Female, Glycoproteins immunology, Leukocyte Rolling, Mice, Mice, Inbred C57BL, Mice, Knockout, Microvessels metabolism, Microvessels pathology, Myelin-Oligodendrocyte Glycoprotein, Peptide Fragments immunology, Receptor, Bradykinin B2 genetics, Chemokine CCL2 immunology, Chemokine CCL5 immunology, Encephalomyelitis, Autoimmune, Experimental immunology, Leukocytes immunology, Receptor, Bradykinin B2 immunology
- Abstract
Background: Kinins are important mediators of inflammation and act through stimulation of two receptor subtypes, B1 and B2. Leukocyte infiltration contributes to the pathogenesis of autoimmune inflammation in the central nervous system (CNS), occurring not only in multiple sclerosis (MS) but also in experimental autoimmune encephalomyelitis (EAE). We have previously shown that the chemokines CCL2 and CCL5 play an important role in the adhesion of leukocytes to the brain microcirculation in EAE. The aim of the present study was to evaluate the relevance of B2 receptors to leukocyte-endothelium interactions in the cerebral microcirculation, and its participation in CNS inflammation in the experimental model of myelin-oligodendrocyte-glycoprotein (MOG)(35-55)-induced EAE in mice., Methods: In order to evaluate the role of B2 receptor in the cerebral microvasculature we used wild-type (WT) and kinin B2 receptor knockout (B2-/-) mice subjected to MOG(35-55)-induced EAE. Intravital microscopy was used to investigate leukocyte recruitment on pial matter vessels in B2-/- and WT EAE mice. Histological documentation of inflammatory infiltrates in brain and spinal cords was correlated with intravital findings. The expression of CCL5 and CCL2 in cerebral tissue was assessed by ELISA., Results: Clinical parameters of disease were reduced in B2-/- mice in comparison to wild type EAE mice. At day 14 after EAE induction, there was a significant decrease in the number of adherent leukocytes, a reduction of cerebral CCL5 and CCL2 expressions, and smaller inflammatory and degenerative changes in B2-/- mice when compared to WT., Conclusion: Our results suggest that B2 receptors have two major effects in the control of EAE severity: (i) B2 regulates the expression of chemokines, including CCL2 and CCL5, and (ii) B2 modulates leukocyte recruitment and inflammatory lesions in the CNS.
- Published
- 2008
- Full Text
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39. Acidic triterpenes compromise growth and survival of astrocytoma cell lines by regulating reactive oxygen species accumulation.
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Martín R, Carvalho-Tavares J, Ibeas E, Hernández M, Ruiz-Gutierrez V, and Nieto ML
- Subjects
- Apoptosis drug effects, Astrocytoma metabolism, Astrocytoma pathology, Brain Neoplasms metabolism, Brain Neoplasms pathology, Cell Growth Processes drug effects, Cell Line, Tumor, Cell Survival drug effects, Cytoskeleton drug effects, Cytoskeleton metabolism, Dose-Response Relationship, Drug, Humans, Membrane Potential, Mitochondrial drug effects, Membrane Potential, Mitochondrial physiology, Astrocytoma drug therapy, Brain Neoplasms drug therapy, Oleanolic Acid pharmacology, Reactive Oxygen Species metabolism, Triterpenes pharmacology
- Abstract
Several studies have shown how pentacyclic triterpenes can inhibit proliferation and induce apoptosis of some tumor cell lines; however, its effect on astrocytic tumors, one of the most malignant forms of cancer, has rarely been reported. The aim of this study was to examine how the pentacyclic triterpenes, oleanolic acid and maslinic acid, isolated from olive juice, affected astrocytoma cell morphology and survival. Cell proliferation was inhibited in 1321N1 astrocytoma cells by using 1 to 50 micromol/L of either oleanolic acid or maslinic acid, with an average IC(50) of 25 micromol/L. Growth inhibition led to morphologic and cytoskeletal alterations associated with the loss of stellate morphology and characterized by a retraction of the cytoplasm and collapse of actin stress fibers. Using 4',6-diamidino-2-phenylindole and Annexin V, we showed that astrocytoma cell death induced by oleanolic acid or maslinic acid were mainly due to apoptotic events. Furthermore, we showed that caspase-3 is activated as a consequence of triterpene treatment. Finally, we found that exposure of the cells to oleanolic acid or maslinic acid resulted in a significant increase of intracellular reactive oxygen species, followed by loss of mitochondrial membrane integrity. Importantly, enzymatic scavengers, such as catalase, or phenolic antioxidants, such as butylated hydroxytoluene, rescued cells from the triterpene-mediated apoptosis, suggesting that the potential therapeutic effect of these acidic triterpenes is dependent on oxidative stress. Our data show that acidic triterpenes play a major role in 1321N1 astrocytoma morphology and viability and support the conclusion that oleanolic acid and maslinic acid may thus be promising new agents in the management of astrocytomas.
- Published
- 2007
- Full Text
- View/download PDF
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