45 results on '"Cao, Chunwei"'
Search Results
2. Understanding recurrent pregnancy loss: recent advances on its etiology, clinical diagnosis, and management
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Cao Chunwei, Bai Shiyu, Zhang Jing, Sun Xiaoyue, Meng Anming, and Chen Hui
- Subjects
etiologic diagnosis ,genetic etiology ,next generation sequencing ,recurrent pregnancy loss ,therapeutic recommendations ,Medicine - Abstract
Recurrent pregnancy loss (RPL) has become an important reproductive health issue worldwide. RPL affects about 2%–3% of reproductive-aged women, and makes serious threats to women’s physical and mental health. However, the etiology of approximately 50% of RPL cases remains unknown (unexplained RPL), which poses a big challenge for clinical management of these patients. RPL has been widely regarded as a complex disease where its etiology has been attributed to numerous factors. Heretofore, various risk factors for RPL have been identified, such as maternal ages, genetic factors, anatomical structural abnormalities, endocrine dysfunction, prethrombotic state, immunological factors, and infection. More importantly, development and applications of next generation sequencing technology have significantly expanded opportunities to discover chromosomal aberrations and single gene variants responsible for RPL, which provides new insight into its pathogenic mechanisms. Furthermore, based upon patients’ diagnostic evaluation and etiologic diagnosis, specific therapeutic recommendations have been established. This review will highlight current understanding and recent advances on RPL, with a special focus on the immunological and genetic etiologies, clinical diagnosis and therapeutic management.
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- 2022
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3. Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling
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Wu, Yaoqiu, Yang, Rong, Lan, Jie, Wu, Yingchen, Huang, Jianyun, Fan, Qi, You, Yang, Lin, Haiyan, Jiao, Xuedan, Chen, Hui, Cao, Chunwei, and Zhang, Qingxue
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- 2023
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4. One-step base editing in multiple genes by direct embryo injection for pig trait improvement
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Song, Ruigao, Wang, Yu, Zheng, Qiantao, Yao, Jing, Cao, Chunwei, Wang, Yanfang, and Zhao, Jianguo
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- 2022
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5. CRISPR/Cas9-mediated correction of MITF homozygous point mutation in a Waardenburg syndrome 2A pig model
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Yao, Jing, Wang, Yu, Cao, Chunwei, Song, Ruigao, Bi, Dengfeng, Zhang, Hongyong, Li, Yongshun, Qin, Guosong, Hou, Naipeng, Zhang, Nan, Zhang, Jin, Guo, Weiwei, Yang, Shiming, Wang, Yanfang, and Zhao, Jianguo
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- 2021
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6. Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis
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Liang, Xiaojuan, Tao, Cong, Pan, Jianfei, Zhang, Lilan, Liu, Lulu, Zhao, Ying, Fan, Yiping, Cao, Chunwei, Liu, Jiali, Zhang, Jin, Lam, Sin Man, Shui, Guanghou, Jin, Wanzhu, Li, Wei, Zhao, Jianguo, Li, Kui, and Wang, Yanfang
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- 2021
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7. Single-cell RNA-sequencing reveals distinct immune cell subsets and signaling pathways in IgA nephropathy
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Zeng, Honghui, Wang, Le, Li, Jiajia, Luo, Siweier, Han, Qianqian, Su, Fang, Wei, Jing, Wei, Xiaona, Wu, Jianping, Li, Bin, Huang, Jingang, Tang, Patrick, Cao, Chunwei, Zhou, Yiming, and Yang, Qiongqiong
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- 2021
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8. Adipocyte-specific disruption of ATPase copper transporting α in mice accelerates lipoatrophy
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Tao, Cong, Wang, Yajun, Zhao, Ying, Pan, Jianfei, Fan, Yiping, Liang, Xiaojuan, Cao, Chunwei, Zhao, Jianguo, Petris, Michael J., Li, Kui, and Wang, Yanfang
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- 2019
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9. Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity
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Zheng, Qiantao, Lin, Jun, Huang, Jiaojiao, Zhang, Hongyong, Zhang, Rui, Zhang, Xueying, Cao, Chunwei, Hambly, Catherine, Qin, Guosong, Yao, Jing, Song, Ruigao, Jia, Qitao, Wang, Xiao, Li, Yongshun, Zhang, Nan, Piao, Zhengyu, Ye, Rongcai, Speakman, John R., Wang, Hongmei, Zhou, Qi, Wang, Yanfang, Jin, Wanzhu, and Zhao, Jianguo
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- 2017
10. The asynchronous establishment of chromatin 3D architecture between in vitro fertilized and uniparental preimplantation pig embryos
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Li, Feifei, Wang, Danyang, Song, Ruigao, Cao, Chunwei, Zhang, Zhihua, Wang, Yu, Li, Xiaoli, Huang, Jiaojiao, Liu, Qiang, Hou, Naipeng, Xu, Bingxiang, Li, Xiao, Gao, Xiaomeng, Jia, Yan, Zhao, Jianguo, and Wang, Yanfang
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- 2020
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11. Thyroid hormone regulates hematopoiesis via the TR-KLF9 axis
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Zhang, Ying, Xue, Yuanyuan, Cao, Chunwei, Huang, Jiaojiao, Hong, Qianlong, Hai, Tang, Jia, Qitao, Wang, Xianlong, Qin, Guosong, Yao, Jing, Wang, Xiao, Zheng, Qiantao, Zhang, Rui, Li, Yongshun, Luo, Ailing, Zhang, Nan, Shi, Guizhi, Wang, Yanfang, Ying, Hao, Liu, Zhonghua, Wang, Hongmei, Meng, Anming, Zhou, Qi, Wei, Hong, Liu, Feng, and Zhao, Jianguo
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- 2017
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12. Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis
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Hai, Tang, Guo, Weiwei, Yao, Jing, Cao, Chunwei, Luo, Ailing, Qi, Meng, Wang, Xianlong, Wang, Xiao, Huang, Jiaojiao, Zhang, Ying, Zhang, Hongyong, Wang, Dayu, Shang, Haitao, Hong, Qianlong, Zhang, Rui, Jia, Qitao, Zheng, Qiantao, Qin, Guosong, Li, Yongshun, Zhang, Tao, Jin, Weiwu, Chen, Zheng-Yi, Wang, Hongmei, Zhou, Qi, Meng, Anming, Wei, Hong, Yang, Shiming, and Zhao, Jianguo
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- 2017
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13. A novel porcine model reproduces human oculocutaneous albinism type II
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Zhang, Ying, Hong, Qianlong, Cao, Chunwei, Yang, Lizhu, Li, Yongshun, Hai, Tang, Zhang, Hongyong, Zhou, Qi, Sui, Ruifang, and Zhao, Jianguo
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- 2019
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14. Cold adaptation in pigs depends on UCP3 in beige adipocytes
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Lin, Jun, Cao, Chunwei, Tao, Cong, Ye, Rongcai, Dong, Meng, Zheng, Qiantao, Wang, Chao, Jiang, Xiaoxiao, Qin, Guosong, Yan, Changguo, Li, Kui, Speakman, John R, Wang, Yanfang, Jin, Wanzhu, and Zhao, Jianguo
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- 2017
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15. Potential contribution of the neurodegenerative disorders risk loci to cognitive performance in an elderly male gout population
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Han, Lin, Jia, Zhaotong, Cao, Chunwei, Liu, Zhen, Liu, Fuqiang, Wang, Lin, Ren, Wei, Sun, Mingxia, Wang, Baoping, Li, Changgui, and Chen, Li
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- 2017
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16. Distinct Transcriptional Responses of Skeletal Muscle to Short-Term Cold Exposure in Tibetan Pigs and Bama Pigs.
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Yang, Chunhuai, Cao, Chunwei, Liu, Jiali, Zhao, Ying, Pan, Jianfei, Tao, Cong, and Wang, Yanfang
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SKELETAL muscle , *COLD adaptation , *BICEPS femoris , *BODY temperature , *ERECTOR spinae muscles , *FATTY acids , *SWINE - Abstract
Piglets are susceptible to cold, and piglet death caused by cold stress leads to economic losses in the pig industry in cold areas. Skeletal muscle plays a key role in adaptive thermogenesis in mammals, but the related mechanism in pigs is unclear. In this study, cold-tolerant Tibetan pigs and cold-sensitive Bama pigs were subjected to either a cold environment (4 °C) or a room temperature environment (25 °C) for 3 days. The biceps femoris (BF) and longissimus dorsi muscle (LDM) were collected for phenotypic analysis, and the BF was used for genome-wide transcriptional profiling. Our results showed that Tibetan pigs had a higher body temperature than Bama pigs upon cold stimulation. RNA-seq data indicated a stronger transcriptional response in the skeletal muscle of Tibetan pigs upon cold stimulation, as more differentially expressed genes (DEGs) were identified with the same criteria (p < 0.05 and fold change > 2). In addition, distinct pathway signaling patterns in skeletal muscle upon cold exposure were found between the breeds of pigs. Mitochondrial beta-oxidation-related genes and pathways were significantly upregulated in Tibetan pigs, indicating that Tibetan pigs may use fatty acids as the primary fuel source to protect against cold. However, the significant upregulation of inflammatory response- and glycolysis-related genes and pathways in the skeletal muscle of Bama pigs suggested that these pigs may use glucose as the primary fuel source in cold environments. Together, our study revealed the distinct transcriptional responses of skeletal muscle to cold stimulation in Tibetan pigs and Bama pigs and provided novel insights for future investigation of the cold adaptation mechanism in pigs. [ABSTRACT FROM AUTHOR]
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- 2023
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17. CTCF and Its Partners: Shaper of 3D Genome during Development.
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Sun, Xiaoyue, Zhang, Jing, and Cao, Chunwei
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GENETIC transcription regulation ,COHESINS ,CELL differentiation ,CHROMATIN ,RNA - Abstract
The 3D genome organization and its dynamic modulate genome function, playing a pivotal role in cell differentiation and development. CTCF and cohesin, acting as the core architectural components involved in chromatin looping and genome folding, can also recruit other protein or RNA partners to fine-tune genome structure during development. Moreover, systematic screening for partners of CTCF has been performed through high-throughput approaches. In particular, several novel protein and RNA partners, such as BHLHE40, WIZ, MAZ, Aire, MyoD, YY1, ZNF143, and Jpx, have been identified, and these partners are mostly implicated in transcriptional regulation and chromatin remodeling, offering a unique opportunity for dissecting their roles in higher-order chromatin organization by collaborating with CTCF and cohesin. Here, we review the latest advancements with an emphasis on features of CTCF partners and also discuss the specific functions of CTCF-associated complexes in chromatin structure modulation, which may extend our understanding of the functions of higher-order chromatin architecture in developmental processes. [ABSTRACT FROM AUTHOR]
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- 2022
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18. A single-nucleotide polymorphism of the STAT4 gene is associated with systemic lupus erythematosus (SLE) in female Chinese population
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Luan, Haixia, Li, Ping, Cao, Chunwei, Li, Chaohua, Hu, Chaojun, Zhang, Shulan, Zeng, Xiaofeng, Zhang, Fengchun, Zeng, Changqing, and Li, Yongzhe
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- 2012
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19. A Validated Model for Individualized Prediction of Live Birth in Patients With Adenomyosis Undergoing Frozen–Thawed Embryo Transfer.
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Wu, Yaoqiu, Yang, Rong, Lin, Haiyan, Cao, Chunwei, Jiao, Xuedan, and Zhang, Qingxue
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FERTILIZATION in vitro ,EMBRYO transfer ,ENDOMETRIOSIS ,MULTIPLE pregnancy ,PREDICTION models ,PUBLIC hospitals - Abstract
Purpose: This study aimed to develop a predictive tool for live birth in women with adenomyosis undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. Methods: A total of 424 patients with adenomyosis who underwent frozen–thawed embryo transfer (FET) from January 2013 to December 2019 at a public university hospital were included. The patients were randomly divided into training (n = 265) and validation (n = 159) samples for the building and testing of the nomogram, respectively. Multivariate logistic regression (MLR) was developed on the basis of clinical covariates assessed for their association with live birth. Results: In total, 183 (43.16%) patients became pregnant, and 114 (26.88%) had a live birth. The MLR showed that the probability of live birth was significantly correlated with age [odds ratio (OR), 3.465; 95% confidence interval (CI), 1.215–9.885, P = 0.020], uterine volume (OR, 8.141; 95% CI, 2.170–10.542; P = 0.002), blastocyst transfer (OR, 3.231; 95% CI, 1.065–8.819, P = 0.023), twin pregnancy (OR, 0.328; 95% CI, 0.104–0.344, P = 0.005), and protocol in FET (P < 0.001). The statistical nomogram was built based on age, uterine volume, twin pregnancy, stage of the transferred embryo, and protocol of FET, with an area under the curve (AUC) of 0.837 (95% CI: 0.741–0.910) for the training cohort. The AUC for the validation cohort was 0.737 (95% CI: 0.661–0.813), presenting a well-pleasing goodness-of-fit and stability in this model. Conclusions: This visual and easily applied nomogram built on the risk factors of live birth in patients with adenomyosis provides useful and precise information for physicians on individualized decision-making during the IVF/ICSI procedure. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Efficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs
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Yao, Jing, Huang, Jiaojiao, Hai, Tang, Wang, Xianlong, Qin, Guosong, Zhang, Hongyong, Wu, Rong, Cao, Chunwei, Xi, Jianzhong Jeff, Yuan, Zengqiang, and Zhao, Jianguo
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- 2014
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21. Association study of STAT4, IRF7, C1q and IKZF1 polymorphisms with systemic lupus erythematosus in Chinese Han population: 0463
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Li, Ping, Cao, Chunwei, Luan, Haixia, Hu, Chaojun, Zhang, Shulan, Li, Lijun, Zeng, Xiaofeng, Zhang, Fengchun, Zeng, Changqing, and Li, Yongzhe
- Published
- 2010
22. RNF20 affects porcine adipocyte differentiation via regulation of mitotic clonal expansion.
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Zhao, Ying, Pan, Jianfei, Cao, Chunwei, Liang, Xiaojuan, Yang, Shulin, Liu, Lulu, Tao, Cong, Zhao, Jianguo, and Wang, Yanfang
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FAT cells ,STAINS & staining (Microscopy) ,SMALL interfering RNA ,ADIPOGENESIS ,UBIQUITIN ligases ,WESTERN immunoblotting ,HISTONES - Abstract
Objectives: RNF20 is recognized as a main E3 ligase for monoubiquitination of histone H2B at lysine 120 (H2Bub). The critical role of RNF20 and H2Bub in various molecular events, such as DNA replication, RNA transcription, and DNA damage response, has been widely investigated and documented. However, its role in porcine adipogenesis remains unknown. In this study, we aimed to clarify the effect of RNF20 on porcine preadipocyte differentiation. Materials and Methods: Backfat tissues from fat‐type pigs (Bama and Meishan) and lean‐type pigs (Yorkshire and Landrace) were collected to detect the expression level of RNF20. Preadipocytes were isolated from Bama piglets and induced to differentiation. Small interfering RNAs were applied to deplete RNF20. Oil Red O staining, quantitative real‐time PCR, RNA‐seq, Western blot analysis, and EdU assays were performed to study the regulatory mechanism of RNF20 during adipogenesis. Results: We found that the expression levels of RNF20 and H2Bub were significantly higher in backfat tissues from fat‐type pigs than in those from lean‐type pigs. Consistently, the significantly induced expression of RNF20 and H2Bub was also observed in porcine differentiated adipocytes. In addition, knockdown of RNF20 greatly inhibited porcine adipogenesis, as evidenced by dramatically decreased lipid droplet formation and lower expression levels of adipogenic transcription masters in RNF20 knockdown cells. Mechanistically, the depletion of RNF20 decreases the cell proliferation and the level of p‐C/EBPβ via the Ras‐Raf‐MEK1/2‐ERK1/2 cascade pathway at the mitotic clonal expansion phase and therefore suppresses cell differentiation. Conclusions: Our results demonstrate that RNF20 is required for porcine preadipocyte differentiation. [ABSTRACT FROM AUTHOR]
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- 2021
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23. Long Noncoding RNA RP11-115N4.1 Promotes Inflammatory Responses by Interacting With HNRNPH3 and Enhancing the Transcription of HSP70 in Unexplained Recurrent Spontaneous Abortion.
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Liu, Meilan, Sun, Xiaoyue, Zhu, Liqiong, Zhu, Menglan, Deng, Kewen, Nie, Xiaolu, Mo, Hanjie, Du, Tao, Huang, Bingqian, Hu, Lihao, Liang, Liuhong, Wang, Dongyan, Luo, Yinger, Yi, Jinling, Zhang, Jianping, Zhong, Xingming, Cao, Chunwei, and Chen, Hui
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LINCRNA ,RECURRENT miscarriage ,MISCARRIAGE ,INFLAMMATION ,PREGNANCY complications ,CELL migration - Abstract
Background: Unexplained recurrent spontaneous abortion (URSA) is a common pregnancy complication and the etiology is unknown. URSA-associated lncRNAs are expected to be potential biomarkers for diagnosis, and might be related to the disease pathogenesis. Objective: To investigate differential lncRNAs in peripheral blood of non-pregnant URSA patients and matched healthy control women and to explore the possible mechanism of differential lncRNAs leading to URSA. Methods: We profiled lncRNAs expression in peripheral blood from 5 non-pregnant URSA patients and 5 matched healthy control women by lncRNA microarray analysis. Functions of URSA-associated lncRNAs were further investigated in vitro. Results: RP11-115N4.1 was identified as the most differentially expressed lncRNA which was highly upregulated in peripheral blood of non-pregnant URSA patients (P = 3.63E-07, Fold change = 2.96), and this dysregulation was further validated in approximately 26.67% additional patients (4/15). RP11-115N4.1 expression was detected in both lymphocytes and monocytes of human peripheral blood, and in vitro overexpression of RP11-115N4.1 decreased cell proliferation in K562 cells significantly. Furthermore, heat-shock HSP70 genes (HSPA1A and HSPA1B) were found to be significantly upregulated upon RP11-115N4.1 overexpression by transcriptome analysis (HSPA1A (P = 4.39E-08, Fold change = 4.17), HSPA1B (P = 2.26E-06, Fold change = 2.99)). RNA pull down and RNA immunoprecipitation assay (RIP) analysis demonstrated that RP11-115N4.1 bound to HNRNPH3 protein directly, which in turn activate heat-shock proteins (HSP70) analyzed by protein-protein interaction and HNRNPH3 knockdown assays. Most importantly, the high expression of HSP70 was also verified in the serum of URSA patients and the supernatant of K562 cells with RP11-115N4.1 activation, and HSP70 in supernatant can exacerbate inflammatory responses in monocytes by inducing IL-6, IL-1β, and TNF-α and inhibit the migration of trophoblast cells, which might associate with URSA. Conclusion: Our results demonstrated that the activation of RP11-115N4.1 can significantly increase the protein level of HSP70 via binding to HNRNPH3, which may modulate the immune responses and related to URSA. Moreover, RP11-115N4.1 may be a novel etiological biomarker and a new therapeutic target for URSA. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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24. TGFB3 downregulation causing chordomagenesis and its tumor suppression role maintained by Smad7.
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Wang, Liang, Guan, Xiaonan, Hu, Qingtao, Wu, Zhen, Chen, Wei, Song, Lairong, Wang, Ke, Tian, Kaibing, Cao, Chunwei, Zhang, Dake, Ma, Junpeng, Tong, Xiangjun, Zhang, Bo, Zhang, Junting, and Zeng, Changqing
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DOWNREGULATION ,CHORDOMA ,REGULATION of growth ,CELL migration ,TUMORS ,CANCER cells - Abstract
Chordoma is a rare bone tumor arising from notochordal remnants, but the underlying mechanism remains elusive. By integrated mRNA and microRNA analyses, we found significant downregulation of TGFB3 along with upregulation of its inhibitor, miR-29 family in chordoma comparing with notochord. Somatic copy number gains of miR-29 loci in chordoma highlighted a mechanism of inactivation of TGFB3 signaling in tumor formation. In zebrafish, knockout and knockdown homologous tgfb3 resulted in a chordoma-like neoplasm. On the other hand, Smad7 negative feedback regulation of transforming growth factor-β (TGF-β) signaling is retentive in chordoma cell UM-Chor1 despite its disruption in most cancer cells (e.g. A549). Therefore, contrary to other cancers, exogenous TGF-β activated Smad7 by downregulating miR-182 and inhibited cell migration and invasion in UM-Chor1. Meanwhile, TGF-β decreased chordoma characteristic protein Brachyury. Altogether, downregulation of TGFB3 causes chordomagenesis, showing a feasible target for therapies. The retention of Smad7 negative regulation may maintain the suppressor role of TGF-β in chordoma. [ABSTRACT FROM AUTHOR]
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- 2021
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25. Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs.
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Wang, Yu, Bi, Dengfeng, Qin, Guosong, Song, Ruigao, Yao, Jing, Cao, Chunwei, Zheng, Qiantao, Hou, Naipeng, Wang, Yanfang, and Zhao, Jianguo
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FUNCTIONAL genomics ,GENOME editing ,BREEDING ,CYTIDINE deaminase ,SWINE ,SINGLE nucleotide polymorphisms - Abstract
Pig is an important agricultural economic animal, providing large amount of meat products. With the development of functional genomics and bioinformatics, lots of genes and functional single nucleotide polymorphisms (SNPs) related to disease resistance and (or) economic traits in pigs have been identified, which provides the targets for genetic improvement by genome editing. Base editors (BEs), combining Cas9 nickase and cytidine or adenine deaminase, achieve all four possible transition mutations (C-to-T, A-to-G, T-to-C, and G-to-A) efficiently and accurately without double strand breaks (DSBs) under the protospacer adjacent motif (PAM) sequence of NGG. However, the NGG PAM in canonical CRISPR-Cas9 can only cover approximately 8.27% in the whole genome which limits its broad application. In the current study, hA3A-BE3-NG system was constructed with the fusion of SpCas9-NG variant and hA3A-BE3 to create C-to-T conversion at NGN PAM sites efficiently. The editing efficiency and scope of hA3A-BE3-NG were confirmed in HEK293T cells and porcine fetal fibroblast (PFF) cells. Results showed that the efficiency of hA3A-BE3-NG was much higher than that of hA3A-BE3 on NGH (H = A, C, or T) PAM sites (21.27 vs. 2.81% at average). Further, nonsense and missense mutations were introduced efficiently and precisely via hA3A-BE3-NG in multiple pig economic trait-related genes (CD163 , APN , MSTN , and MC4R) in PFF cells by one transfection. The current work indicates the potential applications of hA3A-BE3-NG for pyramid breeding studies in livestock. [ABSTRACT FROM AUTHOR]
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- 2020
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26. A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment.
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Wang, Xiao, Cao, Chunwei, Li, Yongshun, Hai, Tang, Jia, Qitao, Zhang, Ying, Zheng, Qiantao, Yao, Jing, Qin, Guosong, Zhang, Hongyong, Song, Ruigao, Wang, Yanfang, Shui, Guanghou, Lam, Sin Man, Liu, Zhonghua, Wei, Hong, Meng, Anming, Zhou, Qi, and Zhao, Jianguo
- Abstract
Harlequin ichthyosis (HI) is a severe genetic skin disorder and caused by mutation in the ATP-binding cassette A12 (ABCA12) gene. The retinoid administration has dramatically improved long-term survival of HI, but improvements are still needed. However, the ABCA12 null mice failed to respond to retinoid treatment, which impedes the development of novel cure strategies for HI. Here we generated an ethylnitrosourea mutagenic HI pig model (named Z9), which carries a novel deep intronic mutation IVS49-727 A>G in the ABCA12 gene, resulting in abnormal mRNA splicing and truncated protein production. Z9 pigs exhibit significant clinical symptom as human patients with HI. Most importantly, systemic retinoid treatment significantly prolonged the life span of the mutant pigs via improving epidermal maturation, decreasing epidermal apoptosis, and triggering the expression of ABCA6. Taken together, this pig model perfectly resembles the clinical symptom and molecular pathology of patients with HI and will be useful for understanding mechanistic insight and developing therapeutic strategies. [ABSTRACT FROM AUTHOR]
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- 2019
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27. H2B ubiquitination: Conserved molecular mechanism, diverse physiologic functions of the E3 ligase during meiosis.
- Author
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Wang, Liying, Cao, Chunwei, Wang, Fang, Zhao, Jianguo, and Li, Wei
- Subjects
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UBIQUITINATION , *UBIQUITIN ligases , *CHROMATIN , *MEIOSIS , *HISTONES - Abstract
RNF20/Bre1 mediated H2B ubiquitination (H2Bub) has various physiologic functions. Recently, we found that H2Bub participates in meiotic recombination by promoting chromatin relaxation during meiosis. We then analyzed the phylogenetic relationships among the E3 ligase for H2Bub, its E2 Rad6 and their partner WW domain-containing adaptor with a coiled-coil (WAC) or Lge1, and found that the molecular mechanism underlying H2Bub is evolutionarily conserved from yeast to mammals. However, RNF20 has diverse physiologic functions in different organisms, which might be caused by the evolutionary divergency of their domain/motif architectures. In the current extra view, we not only elucidate the evolutionarily conserved molecular mechanism underlying H2Bub, but also discuss the diverse physiologic functions of RNF20 during meiosis. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
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28. Functional and Genetic Characterization of Porcine Beige Adipocytes.
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Zhang, Lilan, Hu, Silu, Cao, Chunwei, Chen, Chuanhe, Liu, Jiali, Wang, Yu, Liu, Jianfeng, Zhao, Jianguo, Tao, Cong, and Wang, Yanfang
- Subjects
ANIMAL disease models ,SWINE breeding ,GENE expression profiling ,FAT cells - Abstract
Beige adipocytes are a distinct type of fat cells with a thermogenic activity that have gained substantial attention as an alternative cellular anti-obesity target in humans. These cells may provide an alternative strategy for the genetic selection of pigs with reduced fat deposition. Despite the presence of beige adipocytes in piglets, the molecular signatures of porcine beige adipocytes remain unclear. Here, white and beige adipocytes from Tibetan piglets were primarily cultured and differentiated. Compared to the white adipocytes, the beige adipocytes exhibited a stronger thermogenic capacity. RNA-sequencing-based genome-wide comparative analyses revealed distinct gene expression profiles for white and beige adipocytes. In addition, two genes, integrin alpha-2 (ITGA2) and calponin 1 (CNN1), which were specifically differentially expressed in porcine beige adipocytes, were further functionally characterized using a loss-of-function approach. Our data showed that both genes were involved in differentiation and thermogenesis of porcine beige adipocytes. Collectively, these data furthered our understanding of gene expression in porcine white and beige adipocytes. Elucidating the genetic basis of beige adipogenesis in pigs will pave the way for molecular design breeding in both pigs and large animal models of human diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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29. Association of genetic variations in the STAT4 and IRF7/KIAA1542 regions with systemic lupus erythematosus in a Northern Han Chinese population
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Li, Ping, Cao, Chunwei, Luan, Haixia, Li, Chaohua, Hu, Chaojun, Zhang, Shulan, Zeng, Xiaofeng, Zhang, Fengchun, Zeng, Changqing, and Li, Yongzhe
- Subjects
- *
SYSTEMIC lupus erythematosus , *CHINESE people , *HUMAN genetic variation , *HUMAN population genetics , *GENETIC polymorphisms , *GENETICS of disease susceptibility , *MEDICAL statistics , *GENETICS , *DISEASES - Abstract
Abstract: Systemic lupus erythematosus (SLE) is a prototypic autoimmune disease with complex genetic inheritance. Genome-wide association studies have identified SLE susceptibility variations at the IRF7/KIAA1542 locus and with STAT4 gene in European populations. We decided to investigate the association of single-nucleotide polymorphisms (SNPs) in the IRF7/KIAA1542 region (rs4963128, rs2246614, and rs702966) and in STAT4 (rs7574865 and rs7582694) with SLE disease in a Northern Han Chinese population of 748 patients and 750 healthy controls. Our study indicated a strong association between rs7574865 (odds ratio = 0.68; 95% confidence interval 0.59–0.79; p = 1.57 × 10−6) and SLE and between rs7574865 and the production of anti-Sm antibodies. Additionally, rs4963128 and rs2246614 were correlated with a variety of clinical subphenotypes, such as lupus nephritis, arthritis, and the production of anti-SSA/B autoantibodies, despite a lack of significant association between these two SNPs and SLE disease susceptibility in general. [Copyright &y& Elsevier]
- Published
- 2011
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30. Operation Optimization of Regional Integrated Energy System Considering the Responsibility of Renewable Energy Consumption and Carbon Emission Trading.
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Li, Feng, Lu, Shirong, Cao, Chunwei, and Feng, Jiang
- Subjects
RENEWABLE energy sources ,CARBON emissions ,ENERGY consumption ,EMISSIONS trading ,CARBON offsetting ,ECONOMIC systems ,MICROGRIDS - Abstract
To "bring carbon emissions to a peak by 2030 and to be carbon-neutral by 2060", the role of renewable energy consumption and carbon emission trading are promoted. As an important energy consumer of regional energy system, it is necessary for integrated energy system to ensure the low-carbon economic operation of the system. Combined with the responsibility of renewable energy consumption, green certificate trading mechanism, carbon emission rights trading, and China Certified Emission Reduction (CCER), a regional integrated energy system operation optimization model was proposed. The model aims to minimize the total cost of the system, which included with electric bus, thermal bus, and cold bus. Setting different scenarios for the given example, the results show that the optimized model could effectively reduce the operating costs of the system. Moreover, the results also provide an effective reference for the system's economic and low-carbon operation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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31. Transcriptional Response of Subcutaneous White Adipose Tissue to Acute Cold Exposure in Mice.
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Liang, Xiaojuan, Pan, Jianfei, Cao, Chunwei, Zhang, Lilan, Zhao, Ying, Fan, Yiping, Li, Kui, Tao, Cong, and Wang, Yanfang
- Subjects
WHITE adipose tissue ,ADIPOSE tissues ,FATTY acid oxidation ,IMMUNOREGULATION ,CELL differentiation ,MICE - Abstract
Beige adipose tissue has been considered to have potential applications in combating obesity and its related metabolic diseases. However, the mechanisms of acute cold-stimulated beige formation still remain largely unknown. Here, transcriptional analysis of acute cold-stimulated (4 °C for 4 h) subcutaneous white adipose tissue (sWAT) was conducted to determine the molecular signatures that might be involved in beige formation. Histological analysis confirmed the appearance of beige adipocytes in acute cold-treated sWAT. The RNA-sequencing data revealed that 714 genes were differentially expressed (p-value < 0.05 and fold change > 2), in which 221 genes were upregulated and 493 genes were downregulated. Gene Ontology (GO) analyses showed that the upregulated genes were enriched in the GO terms related to lipid metabolic process, fatty acid metabolic process, lipid oxidation, fatty acid oxidation, etc. In contrast, downregulated genes were assigned the GO terms of regulation of immune response, regulation of response to stimulus, defense response, etc. The expressions of some browning candidate genes were validated in cold-treated sWAT and 3T3-L1 cell browning differentiation. In summary, our results illustrated the transcriptional response of sWAT to acute cold exposure and identified the genes, including Acad11, Cyp2e1, Plin5, and Pdk2, involved in beige adipocyte formation in mice. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
32. Rescuing ocular development in an anophthalmic pig by blastocyst complementation.
- Author
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Zhang, Hongyong, Huang, Jiaojiao, Li, Zechen, Qin, Guosong, Zhang, Nan, Hai, Tang, Hong, Qianlong, Zheng, Qiantao, Zhang, Ying, Song, Ruigao, Yao, Jing, Cao, Chunwei, Zhao, Jianguo, and Zhou, Qi
- Abstract
Porcine‐derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end‐stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complementation strategy opens a new route for regenerating allogenic organs in miniature pigs. Since the eye is a complicated organ with highly specialized constituent tissues derived from different primordial cell lineages, the development of an intact eye from allogenic cells is a challenging task. Here, combining somatic cell nuclear transfer technology (SCNT) and an anophthalmic pig model (MITFL247S/L247S), allogenic retinal pigmented epithelium cells (RPEs) were retrieved from an E60 chimeric fetus using blastocyst complementation. Furthermore, all structures were successfully regenerated in the intact eye from the injected donor blastomeres. These results clearly demonstrate that not only differentiated functional somatic cells but also a disabled organ with highly specialized constituent tissues can be generated from exogenous blastomeres when delivered to pig embryos with an empty organ niche. This system may also provide novel insights into ocular organogenesis. Synopsis: Blastocyst complementation is a new route for regenerating allogeneic organs in pigs for xenotransplantation, but reconstituting complicated organs, like a whole eye, was never done up to now. This strategy offers potentials for generating personalized human patient‐specific organs in large animals. Intact allogenic eyes can be regenerated using exogenous blastocyst complementation in an anophthalmic pig model.Porcine RPEs are isolated and cultured in vitro from 60‐day chimeric foetuses.The chimeric pig with regenerated eyes was derived from the somatic cloned blastocyst complementation and developed to full term. Blastocyst complementation is a new route for regenerating allogeneic organs in pigs for xenotransplantation, but reconstituting complicated organs, like a whole eye, was never done up to now. This strategy offers potentials for generating personalized human patient‐specific organs in large animals. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
33. Adipose lipidomics and RNA-Seq analysis revealed the enhanced mitochondrial function in UCP1 knock-in pigs.
- Author
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Pan, Jianfei, Tao, Cong, Cao, Chunwei, Zheng, Qiantao, Lam, Sin Man, Shui, Guanghou, Liu, Xuexue, Li, Kui, Zhao, Jianguo, and Wang, Yanfang
- Subjects
- *
LIPID metabolism , *WHITE adipose tissue , *ADIPOSE tissues , *LIPID synthesis , *UNCOUPLING proteins , *SPHINGOLIPIDS - Abstract
Uncoupling protein 1 (UCP1) plays a key role in nonshivering thermogenesis and is involved in the pathogenesis of obesity. In a previous study, we generated adipocyte-specific UCP1 knock-in (UCP1 -KI) pigs, which exhibited improved thermoregulatory ability and decreased fat deposition. To investigate whether UCP1 knock-in alters the lipid composition of adipose tissues, lipidomics of inguinal subcutaneous white adipose tissue (iWAT) and backfat from 6-month-old cold-treated UCP1 -KI pigs and wild-type (WT) pigs were profiled. In addition, genome-wide RNA-sequencing of iWAT was performed to further study the genetic basis for lipid alterations. The results showed that iWAT and backfat from UCP1- KI pigs exhibited distinct lipidomic profiles, as the mild lipid alteration was observed in backfat of UCP1 knock-in pigs. Inguinal WAT from UCP1 -KI pigs contained significantly decreased total triacylglycerol (p < 0.05), together with the downregulation of genes involved in fatty acid metabolism, suggesting the decreased lipogenesis in iWAT of UCP1 -KI pigs. Significantly increased levels of total sphingolipids (p <0.05) were also observed in iWAT from UCP1 -KI pigs. Notably, two mitochondrial-specific lipid species, cardiolipin CL72:8 (18:2) and CL74:9 (18:2), were found to be dramatically increased in iWAT from UCP1 -KI pigs, suggesting enhanced mitochondrial function. This observation was further supported by the significant upregulation of numerous mitochondrial-related genes and significantly increased number of large mitochondria and mitochondrial cristae in iWAT of UCP1 -KI pigs. Taken together, these data illustrate the specific role of UCP1 in lipid metabolism of fat tissues in pigs and provide new data for characterization of fat traits in UCP1 -KI pigs. • Inguinal subcutaneous white adipose tissue (iWAT) and backfat from UCP1- KI pigs exhibited distinct lipidomic profiles. • Significant reductions of total TAG in iWAT from UCP1 -KI pigs, together with the significantly downregulated fatty acid metabolism related genes, indicate the decreased lipid synthesis in iWAT of UCP1 -KI pigs. • Mitochondrial function was enhanced in iWAT of UCP1 -KI pigs, as evidenced by the significantly increased cardiolipin levels, upregulated numerous mitochondrial genes, increased number of large mitochondria and mitochondrial cristae density. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
34. Epidermal growth factor gene is a newly identified candidate gene for gout.
- Author
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Han, Lin, Cao, Chunwei, Jia, Zhaotong, Liu, Shiguo, Liu, Zhen, Xin, Ruosai, Wang, Can, Li, Xinde, Ren, Wei, Wang, Xuefeng, and Li, Changgui
- Published
- 2016
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- View/download PDF
35. One-step generation of triple gene-targeted pigs using CRISPR/Cas9 system.
- Author
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Wang, Xianlong, Cao, Chunwei, Huang, Jiaojiao, Yao, Jing, Hai, Tang, Zheng, Qiantao, Wang, Xiao, Zhang, Hongyong, Qin, Guosong, Cheng, Jinbo, Wang, Yanfang, Yuan, Zengqiang, Zhou, Qi, Wang, Hongmei, and Zhao, Jianguo
- Published
- 2016
- Full Text
- View/download PDF
36. Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs.
- Author
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Wang, Xianlong, Zhou, Jinwei, Cao, Chunwei, Huang, Jiaojiao, Hai, Tang, Wang, Yanfang, Zheng, Qiantao, Zhang, Hongyong, Qin, Guosong, Miao, Xiangnan, Wang, Hongmei, Cao, Suizhong, Zhou, Qi, and Zhao, Jianguo
- Subjects
SWINE genetics ,FIBROBLAST growth factors ,PALINDROMIC DNA ,MUTAGENESIS ,SOMATIC cells - Abstract
Genetic engineering in livestock was greatly enhanced by the emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), which can be programmed with a single-guide RNA (sgRNA) to generate site-specific DNA breaks. However, the uncertainties caused by wide variations in sgRNA activity impede the utility of this system in generating genetically modified pigs. Here, we described a single blastocyst genotyping system to provide a simple and rapid solution to evaluate and compare the sgRNA efficiency at inducing indel mutations for a given gene locus. Assessment of sgRNA mutagenesis efficiencies can be achieved within 10 days from the design of the sgRNA. The most effective sgRNA selected by this system was successfully used to induce site-specific insertion through homology-directed repair at a frequency exceeding 13%. Additionally, the highly efficient gene deletion via the selected sgRNA was confirmed in pig fibroblast cells, which could serve as donor cells for somatic cell nuclear transfer. We further showed that direct cytoplasmic injection of Cas9 mRNA and the favorable sgRNA into zygotes could generate biallelic knockout piglets with an efficiency of up to 100%. Thus, our method considerably reduces the uncertainties and expands the practical possibilities of CRISPR/Cas9-mediated genome engineering in pigs. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
37. Erratum to “Association of genetic variations in the STAT4 and IRF7/KIAA1542 regions with systemic lupus erythematosus in a Northern Han Chinese population” [Human Immunology 72 (2011) 249–255].
- Author
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Li, Ping, Cao, Chunwei, Luan, Haixia, Li, Chaohua, Hu, Chaojun, Zhang, Shulan, Zeng, Xiaofeng, Zhang, Fengchun, Zeng, Changqing, and Li, Yongzhe
- Published
- 2013
- Full Text
- View/download PDF
38. PPARγ is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation.
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Song, Chunlei, Yao, Jing, Cao, Chunwei, Liang, Xiaojuan, Huang, Jiaojiao, Han, Ziqiang, Zhang, Yang, Qin, Guosong, Tao, Cong, Li, Chengbo, Yang, Haoran, Zhao, Jianguo, Li, Kui, and Wang, Yanfang
- Subjects
- *
MICRORNA , *GERMINAL vesicles , *POLYMERASE chain reaction , *FATTY acids , *ROSIGLITAZONE - Abstract
To elucidate the key miRNAs and the signalling pathways that are involved in porcine oocyte maturation, we performed a deep sequencing analysis of the miRNAs of pig germinal vesicle (GV) oocytes and metaphase II (MII) oocytes. Seven differentially expressed (DE) miRNAs were identified and the expression levels of miR-21 and miR-27b-3p were further confirmed by QPCR analysis. The target genes of 7 DE miRNAs were predicted and subjected to pathway analysis. Interestingly, fatty acid metabolism and fatty acid biosynthesis were the top two significantly enriched molecular functions during oocyte maturation. Heat map, which was built with 7 DE miRNAs and the enriched the molecular functions, revealed that miR-21, miR-27b-3p, miR-10a-5p and miR-10b-5p were involved in fatty acid metabolism. In particular, the regulatory role of miR-27b-3p on peroxisome proliferator-activated receptor-γ (PPARγ) was confirmed by their inversed expression patterns in GV and MII oocytes and luciferase report assays. In addition, we observed that PPARγ agonist (rosiglitazone) treatment significantly enhanced porcine oocyte maturation rate and early embryo developmental competent. Taken together, our results demonstrated that miR-27b and its target, PPARγ, play the vital roles in pig oocyte maturation through regulating the fatty acid metabolism. These data increased our understanding of the regulatory gene networks in porcine oocyte maturation and development. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
39. Genetic variants of TREML2 are associated with HLA-B27-positive ankylosing spondylitis.
- Author
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Feng, Yuan, Hong, Yaqiang, Zhang, Xin, Cao, Chunwei, Yang, Xichao, Lai, Shujuan, Fan, Chunmei, Cheng, Feng, Yan, Mei, Li, Chaohua, Huang, Wan, Chen, Wei, Zhu, Ping, and Zeng, Changqing
- Subjects
- *
ANKYLOSING spondylitis , *GENE expression , *SPONDYLITIS , *GENE mapping , *GENETICS , *PATIENTS , *THERAPEUTICS - Abstract
Although ankylosing spondylitis (AS) is a common, highly heritable arthropathy, the precise genetic mechanism underlying the disease remains elusive. Here, we investigate the disease-causing mutations in a large AS family with distinguished complexity, consisting of 23 patients covering four generations and exhibiting a mixed HLA-B27 (+) and (−) status. Linkage analysis with 32 members using three methods and whole-exome sequencing analysis with three HLA-B27 (+) patients, one HLA-B27 (−) patient, and one healthy individual did not identify a mutation common to all of the patients, strongly suggesting the existence of genetic heterogeneity in this large pedigree. However, if only B27-positive patients were analyzed, the linkage analysis located a 22-Mb region harboring the HLA gene cluster in chromosome 6 (LOD = 4.2), and the subsequent exome analysis identified two non-synonymous mutations in the TREML2 and IP6K3 genes. These genes were resequenced among 370 sporadic AS patients and 487 healthy individuals. A significantly higher mutation frequency of TREML2 was observed in AS patients (1.51% versus 0.21%). The results obtained for the AS pedigree and sporadic patients suggest that mutation of TREML2 is a major factor leading to AS for HLA-B27 (+) members in this large family and that TREML2 is also a susceptibility gene promoting the development of ankylosing spondylitis in HLA-B27 (+) individuals. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
40. T gene isoform expression pattern is significantly different between chordomas and notochords.
- Author
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Wang, Ke, Hu, Qingtao, Wang, Liang, Chen, Wei, Tian, Kaibing, Cao, Chunwei, Wu, Zhen, Jia, Guijun, Zhang, Liwei, Zeng, Changqing, and Zhang, Junting
- Subjects
- *
CHORDOMA , *GENE expression , *NOTOCHORD , *SKULL base , *SINGLE nucleotide polymorphisms , *WESTERN immunoblotting - Abstract
The T gene plays a key role in chordoma pathology. To investigate the role of T gene isoforms in chordoma, 22 skull base chordomas, three chordoma cell lines and 9 infant notochords, which were used as normal controls, were collected. We first conducted droplet digital PCR to quantify the absolute expression levels of the long and short isoforms of the T gene (T-long and T-short, respectively) and revealed that T-long was dominantly expressed in all chordomas and chordoma cell lines, but not in the notochords. The T-long/T-short ratio was significantly different between the chordomas and the notochords. Next, we validated the isoform expression pattern at protein expression level using Western blot in 9 chordomas. Furthermore, the T gene single nucleotide polymorphism site rs2305089, which is the only marker reported to be associated with chordomas, was sequenced in all of the chordoma samples. Association between rs2305089 and T-long/T-short ratio was not significant, indicating it was not involved in T gene alternative splicing. In conclusion, two T gene isoforms were investigated in skull base chordomas and chordoma cell lines, and the longer isoform was dominantly expressed. The distinct expression patterns of these T gene isoforms may contribute to the pathogenesis of skull base chordomas. However, further studies on the function of these isoforms are needed. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
41. An exonic splicing enhancer mutation in DUOX2 causes aberrant alternative splicing and severe congenital hypothyroidism in Bama pigs.
- Author
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Cao C, Zhang Y, Jia Q, Wang X, Zheng Q, Zhang H, Song R, Li Y, Luo A, Hong Q, Qin G, Yao J, Zhang N, Wang Y, Wang H, Zhou Q, and Zhao J
- Subjects
- Animals, Base Sequence, Enhancer Elements, Genetic genetics, Ethylnitrosourea, Female, Genes, Recessive, Genome-Wide Association Study, HeLa Cells, Homozygote, Humans, Hydrogen Peroxide metabolism, Inheritance Patterns genetics, Male, Pedigree, RNA, Messenger genetics, RNA, Messenger metabolism, Thyroid Hormones deficiency, Thyroid Hormones metabolism, Exome Sequencing, Alternative Splicing genetics, Congenital Hypothyroidism genetics, Dual Oxidases genetics, Exons genetics, Mutation genetics, Swine genetics
- Abstract
Pigs share many similarities with humans in terms of anatomy, physiology and genetics, and have long been recognized as important experimental animals in biomedical research. Using an N-ethyl-N-nitrosourea (ENU) mutagenesis screen, we previously identified a large number of pig mutants, which could be further established as human disease models. However, the identification of causative mutations in large animals with great heterogeneity remains a challenging endeavor. Here, we select one pig mutant, showing congenital nude skin and thyroid deficiency in a recessive inheritance pattern. We were able to efficiently map the causative mutation using family-based genome-wide association studies combined with whole-exome sequencing and a small sample size. A loss-of-function variant (c.1226 A>G) that resulted in a highly conserved amino acid substitution (D409G) was identified in the DUOX2 gene. This mutation, located within an exonic splicing enhancer motif, caused aberrant splicing of DUOX2 transcripts and resulted in lower H
2 O2 production, which might cause a severe defect in thyroid hormone production. Our findings suggest that exome sequencing is an efficient way to map causative mutations and that DUOX2D409G/D409G mutant pigs could be a potential large animal model for human congenital hypothyroidism., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2019. Published by The Company of Biologists Ltd.)- Published
- 2019
- Full Text
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42. Pilot study of large-scale production of mutant pigs by ENU mutagenesis.
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Hai T, Cao C, Shang H, Guo W, Mu Y, Yang S, Zhang Y, Zheng Q, Zhang T, Wang X, Liu Y, Kong Q, Li K, Wang D, Qi M, Hong Q, Zhang R, Wang X, Jia Q, Wang X, Qin G, Li Y, Luo A, Jin W, Yao J, Huang J, Zhang H, Li M, Xie X, Zheng X, Guo K, Wang Q, Zhang S, Li L, Xie F, Zhang Y, Weng X, Yin Z, Hu K, Cong Y, Zheng P, Zou H, Xin L, Xia J, Ruan J, Li H, Zhao W, Yuan J, Liu Z, Gu W, Li M, Wang Y, Wang H, Yang S, Liu Z, Wei H, Zhao J, Zhou Q, and Meng A
- Subjects
- Animals, China, Pilot Projects, Ethylnitrosourea metabolism, Genetic Association Studies methods, Mutagenesis, Mutagens metabolism, Swine genetics
- Abstract
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on a large scale efficiently, and to discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish and mice, but it has never been tried in large model animals. We describe a successful systematic three-generation ENU mutagenesis screening in pigs with the establishment of the Chinese Swine Mutagenesis Consortium. A total of 6,770 G1 and 6,800 G3 pigs were screened, 36 dominant and 91 recessive novel pig families with various phenotypes were established. The causative mutations in 10 mutant families were further mapped. As examples, the mutation of SOX10 (R109W) in pig causes inner ear malfunctions and mimics human Mondini dysplasia, and upregulated expression of FBXO32 is associated with congenital splay legs. This study demonstrates the feasibility of artificial random mutagenesis in pigs and opens an avenue for generating a reservoir of mutants for agricultural production and biomedical research.
- Published
- 2017
- Full Text
- View/download PDF
43. A 2-bp insertion (c.67_68insCC) in MC1R causes recessive white coat color in Bama miniature pigs.
- Author
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Jia Q, Cao C, Tang H, Zhang Y, Zheng Q, Wang X, Zhang R, Wang X, Luo A, Wei H, Meng A, Zhou Q, Wang H, and Zhao J
- Subjects
- Animals, Base Sequence, Phenotype, Swine, Mutagenesis, Insertional, Pigmentation genetics, Receptor, Melanocortin, Type 1 genetics, Swine, Miniature anatomy & histology, Swine, Miniature genetics
- Published
- 2017
- Full Text
- View/download PDF
44. BIX-01294 increases pig cloning efficiency by improving epigenetic reprogramming of somatic cell nuclei.
- Author
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Huang J, Zhang H, Yao J, Qin G, Wang F, Wang X, Luo A, Zheng Q, Cao C, and Zhao J
- Subjects
- Animals, Blastocyst chemistry, Blastocyst physiology, Cellular Reprogramming genetics, Embryo Culture Techniques veterinary, Embryo Transfer veterinary, Embryonic Development drug effects, Female, Gene Expression drug effects, Histone-Lysine N-Methyltransferase antagonists & inhibitors, Histones metabolism, Homeodomain Proteins genetics, Octamer Transcription Factor-3 genetics, Pluripotent Stem Cells, RNA, Messenger analysis, SOXB1 Transcription Factors genetics, Azepines pharmacology, Cellular Reprogramming drug effects, Cloning, Organism methods, Epigenesis, Genetic, Nuclear Transfer Techniques veterinary, Quinazolines pharmacology, Sus scrofa
- Abstract
Accumulating evidence suggests that faulty epigenetic reprogramming leads to the abnormal development of cloned embryos and results in the low success rates observed in all mammals produced through somatic cell nuclear transfer (SCNT). The aberrant methylation status of H3K9me and H3K9me2 has been reported in cloned mouse embryos. To explore the role of H3K9me2 and H3K9me in the porcine somatic cell nuclear reprogramming, BIX-01294, known as a specific inhibitor of G9A (histone-lysine methyltransferase of H3K9), was used to treat the nuclear-transferred (NT) oocytes for 14-16 h after activation. The results showed that the developmental competence of porcine SCNT embryos was significantly enhanced both in vitro (blastocyst rate 16.4% vs 23.2%, P<0.05) and in vivo (cloning rate 1.59% vs 2.96%) after 50 nm BIX-01294 treatment. BIX-01294 treatment significantly decreased the levels of H3K9me2 and H3K9me at the 2- and 4-cell stages, which are associated with embryo genetic activation, and increased the transcriptional expression of the pluripotency genes SOX2, NANOG and OCT4 in cloned blastocysts. Furthermore, the histone acetylation levels of H3K9, H4K8 and H4K12 in cloned embryos were decreased after BIX-01294 treatment. However, co-treatment of activated NT oocytes with BIX-01294 and Scriptaid rescued donor nuclear chromatin from decreased histone acetylation of H4K8 that resulted from exposure to BIX-01294 only and consequently improved the preimplantation development of SCNT embryos (blastocyst formation rates of 23.7% vs 21.5%). These results indicated that treatment with BIX-01294 enhanced the developmental competence of porcine SCNT embryos through improvements in epigenetic reprogramming and gene expression., (© 2016 Society for Reproduction and Fertility.)
- Published
- 2016
- Full Text
- View/download PDF
45. Association of GTF2I and GTF2IRD1 polymorphisms with systemic lupus erythematosus in a Chinese Han population.
- Author
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Li Y, Li P, Chen S, Wu Z, Li J, Zhang S, Cao C, Wang L, Liu B, Zhang F, and Li YZ
- Subjects
- Adult, Case-Control Studies, China epidemiology, Female, Gene Frequency, Genetic Association Studies, Genetic Predisposition to Disease, Humans, Lupus Erythematosus, Systemic diagnosis, Lupus Erythematosus, Systemic ethnology, Male, Middle Aged, Phenotype, Risk Factors, Young Adult, Asian People genetics, Lupus Erythematosus, Systemic genetics, Muscle Proteins genetics, Nuclear Proteins genetics, Polymorphism, Single Nucleotide, Trans-Activators genetics, Transcription Factors, TFII genetics
- Abstract
Objectives: Systemic lupus erythematosus (SLE) is the most common systemic autoimmune disease which likely involves complex interactions between genes and the environment. Two large-scale genome-wide association studies (GWAS) have implicated many loci as genetic risk factors associated with primary Sjögren's syndrome (pSS). Among them there are a number of pSS associated gene polymorphisms including the MHC-II, STAT4, IRF5, BLK, and TNIP1 genes that are shared with SLE. However, the association of other genes such as GTF2I, GTF2IRD1, and IL12A with SLE remain unknown. This study aimed to determine whether single nucleotide polymorphisms (SNPs) in GTF2I, GTF2IRD1 or IL12A genetically predispose a Chinese Han population to SLE., Methods: Four SNPs in the GTF2I region (rs117026326), the GTF2IRD1 region (rs4717901), and the IL12A region (rs485497, rs583911) were genotyped in a cohort of 948 SLE patients and 938 healthy controls, using the polymerase chain reaction-ligation detection reaction (PCR-LDR) method., Results: he frequency of risk allele of rs117026326 was notably higher in SLE patients than in controls (37.2% vs. 14.9%, OR: 3.39, 95%CI: 2.89-3.97, pc =3.31×10-54). Similarly, rs4717901 was also associated with SLE (35.3% vs. 20.2%, OR: 2.16, 95%CI: 1.86-2.50, pc =1.50×10-24). The frequencies of alleles and genotypes of IL12A SNPs were not significantly different between the SLE patients and controls., Conclusions: This study demonstrates a significant association between SLE and the GTF2I rs117026326 T allele, GTF2IRD1 rs4717901 C allele. The association of GTF2I and GTF2IRD1 as common genetic susceptibility factor in SLE will require further validation in other ethnic lines.
- Published
- 2015
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