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Objective: to study the evolution of the industrial policy of the Union State of Russia and Belarus from 1999 to 2024. Methods: analysis of strategic documents, statistical analysis of macroeconomic indicators and study of regional aspects of economic integration. We studied the statistics of joint ventures with Belarusian capital in Russia and with Russian capital in Belarus, which indicates the changing economic conditions in the Union State. Results: the industrial policy of the Union State is systematically developing and is aimed at deepening the economic integration of the two countries. It relies on the implementation of strategic agreements, planning and financing of joint scientific and technical programs. The study shows that despite the existence of strategic agreements, such as the 1999 Agreement on a Common Structural Industrial Policy and the 2023 Agreement on a Common Industrial Policy, a significantpart of the planned activities and projects has remained only partially implemented. The article elaborates on the key changes in the content of industrial policy, including increased emphasis on integration projects, import substitution and development of new types of competitive products. The study of the statistics of joint ventures with Belarusian capital in Russia and with Russian capital in Belarus points to changing economic conditions in the Union State. Since 2017, the number of joint ventures has been decreasing, which can be a consequence of both the reduction of barriers in international trade and economy and the decreased interest of enterprises in bilateral relations. The industrial policy of the Union State is currently aimed at overcoming dependence on the supply of foreign equipment and components, creating a common economic and scientific-technical space, financing and developing scientific-industrial cooperation based on union programs and projects. Scientific novelty: it consists in analyzing the evolution of the common industrial policy of the Union State for more than two decades. For the first time such a detailed analysis covered the whole period from the signing of the initial agreement in 1999 to the last intergovernmental agreement in 2023. This allows getting a holistic view of the development of approachesand transformation of the Union State industrial policy. Practical significance: for the first time, the study provides an insight into the dynamics of economic integration of the Russian and Belarus regions within the Union State through the statistics of joint Russian-Belarusian enterprises in the regions. This can be useful for researchers, business community and authorities to understand the processes of industrial policy impact on the real sector of the economy.

Mycoplasma bovis is considered one of bovine mycoplasmosis pathogens responsible for respiratory diseases, mastitis, arthritis and keratoconjunctivitis. The paper presents results of the study on optimizing the component composition of the culture medium for Mycoplasma bovis “Kaluga 2020” isolate, as well as the study of this pathogen’s growth stages. The color-changing units assay and the culture method combined with colony-forming unit quantification were used for determination of Mycoplasma activity. It was found that when cultured in an optimized nutrient medium based on modified Hayflick broth, the microorganism enters a logarithmic growth phase after first 24 hours ofgrowth, in 72 hours the Mycoplasma culture enters astability phase, and adecline phase is recorded in 84 hours. The effect of percentage content of glucose, fresh yeast extract and horse serum in the nutrient medium on accumulation of Mycoplasma bovis “Kaluga2020” isolate was evaluated using the one-factor-at-a-time approach. It was found that the greatest effect on Mycoplasma accumulation was exerted by such growth factors as fresh yeast extract and horse serum in the nutrient medium (p < 0.05), while changes in the amount of glucose did not stimulate Mycoplasma bovis growth. Based on results of the conducted studies, the appropriate composition was determined and the optimal content of growth factors in the medium for culturing Mycoplasma bovis “Kaluga 2020” isolate was selected: 12.5%of fresh yeast extract and 25% of horse serum. The use of the optimized nutrient medium based on modified Hayflick broth allowed 5-fold increase in accumulation of Mycoplasma biomass (3.98 × 109CFU/ml)compared to the standard medium (0.79 × 109CFU/ml).

In density estimation, generative models are usually categorized under unsupervised learning due to the lack of labeled data. These models apply various indirect loss functions to refine neural network training, yet face specific challenges. Issues like mode collapse and instability in generative adversarial networks are notable, while normalizing flows are constrained by the need to calculate the Jacobian matrix's determinant, limiting network design. While neural networks are well-suited for handling very high-dimensional data, they can be overly complex for moderately high dimensions. Here, traditional sparse polynomial approximation offers advantages by avoiding complex training requirements. This research employs a score-based diffusion model combined with sparse grid interpolation to estimate the unknown density function. The method involves generating labeled data pairs linking samples from the standard Gaussian distribution to the target distribution using the diffusion model. This model transports the Gaussian distribution to the target density through a backward stochastic differential equation, where a Monte Carlo method approximates the score function at any point, facilitating function interpolation for the transport model. A sparse grid interpolant can be built based on the labeled data. We leverage the Tasmanian library [32] for building this sparse-grid-based generative model. We demonstrate the performance of our method using a set of multi-dimensional benchmark distributions. [ABSTRACT FROM AUTHOR]

Several strains of a Gram-negative, anaerobic photoautotrophic, motile, rod-shaped bacterium, designated as B14B, A-7R, and A-7Y were isolated from biofilms of low-mineralized soda lakes in central Mongolia and Russia (southeast Siberia). They had lamellar stacks as photosynthetic structures and bacteriochlorophyll a as the major photosynthetic pigment. The strains were found to grow at 25–35 °C, pH 7.5–10.2 (optimum, pH 9.0), and with 0–8% (w/v) NaCl (optimum, 0%). In the presence of sulfide and bicarbonate, acetate, butyrate, yeast extract, lactate, malate, pyruvate, succinate, and fumarate promoted growth. The DNA G + C content was 62.9–63.0 mol%. While the 16S rRNA gene sequences confirmed that the new strains belonged to the genus Ectothiorhodospira of the Ectothiorhodospiraceae, comparison of the genome nucleotide sequences of strains B14B, A-7R, and A-7Y revealed that the new isolates were remote from all described Ectothiorhodospira species both in dDDH (19.7–38.8%) and in ANI (75.0–89.4%). The new strains are also genetically differentiated by the presence of a nitric oxide reduction pathway that is lacking from all other Ectiothiorhodospiraceae. We propose to assign the isolates to the new species, Ectothiorhodospira lacustris sp. nov., with the type strain B14BT (=DSM 116064T = KCTC 25542T = UQM 41491T).

See the retraction notice E3S Web of Conferences 420, 00001 (2023), https://doi.org/10.1051/e3sconf/202342000001

Currently, among the various areas of targeted wastewater treatment, great attention is being given by researchers to the solid-phase extraction of organic dyes using metal–organic frameworks (MOFs). In this work, a mixed-ligand Zr-MOF containing terephthalic acid and 1,10-phenanthroline as linkers was used for this purpose. The limiting adsorption of the dyes Congo red and methylene blue, according to experimental data, is 40 mg/g. The influence of various parameters (time, temperature, adsorbent dosage, pH, and coexisting ions) on adsorption characteristics was studied. The sorbent was tested for the removal of dyes from drinks in water and in artificial seawater. The possibility of the separation of dyes by column chromatography using a sorbent as a filler was studied.

A key event in the cytochrome c-dependent apoptotic pathway is the permeabilization of the outer mitochondrial membrane, resulting in the release of various apoptogenic factors, including cytochrome c, into the cytosol. It is believed that the permeabilization of the outer mitochondrial membrane can be induced by the peroxidase activity of cytochrome c in a complex with cardiolipin. Using a number of mutant variants of cytochrome c, we showed that both substitutions of Lys residues from the universal binding site for oppositely charged Glu residues and mutations leading to a decrease in the conformational mobility of the red Ω-loop in almost all cases did not affect the ability of cytochrome c to bind to cardiolipin. At the same time, the peroxidase activity of all mutant variants in a complex with cardiolipin was three to five times higher than that of the wild type. A pronounced increase in the ability to permeabilize the lipid membrane in the presence of hydrogen peroxide, as measured by calcein leakage from liposomes, was observed only in the case of four substitutions in the red Ω-loop (M4 mutant). According to resonance and surface-enhanced Raman spectroscopy, the mutations caused significant changes in the heme of oxidized cytochrome c molecules resulting in an increased probability of the plane heme conformation and the enhancement of the rigidity of the protein surrounding the heme. The binding of wild-type and mutant forms of oxidized cytochrome c to cardiolipin-containing liposomes caused the disordering of the acyl lipid chains that was more pronounced for the M4 mutant. Our findings indicate that the Ω-loop is important for the pore formation in cardiolipin-containing membranes.

Abstract ‘Candidatus Chloroploca asiatica’ B7–9 and ‘Candidatus Viridilinea mediisalina’ Kir15-3F are mesophilic filamentous anoxygenic phototrophic bacteria from alkaline aquatic environments. Both bacteria became available in the last few years and only in stable enrichment culture. In this study, we report the draft genomic sequences of ‘Ca. Chloroploca asiatica’ B7–9 and ‘Ca. Viridilinea mediisalina’ Kir15-3F, which were assembled from metagenomes of their cultures with a fold coverage 86.3× and 163.8×, respectively. The B7–9 (5.8 Mb) and the Kir15-3F (5.6 Mb) draft genome harbors 4818 and 4595 predicted protein-coding genes, respectively. In this article, we analyzed the phylogeny of representatives of the Chloroflexineae suborder in view of the appearance of new genomic data. These data were used for the revision of earlier published group-specific conserved signature indels and for searching for novel signatures for taxons in the Chloroflexineae suborder.

The article presents the results obtained by field tectonophysical methods applied to study tectonic stresses of the Northern Eurasia regions, including young and ancient platforms (West European, Timan–Pechora, Turan, West Siberian, East European, and East Siberian) and orogenic frame structures (Caucasus, Northern Tien Shan, Mongolia-Okhotsk system of mesozoids, and Sakhalin Island). Tectonic stress reconstructions provided the basis for analysing the influence of spreading in the North Atlantics and the Arctic on the stress state of the platforms in Northern Europe. A spatial boundary of the influence goes approximately along the margins of the Fennoscandian shield and the Russian plate in the north. Further southwards, the boundary is submeridional and extends from the western wing of the Byelorussian anteclise almost to the Eastern Carpathians. The stress reconstructions for this boundary show the WNW and W-E-trending axes of compression. The boundary line does not coincide with the Teisser-Tornquist line that represents the boundary between the platforms with heterochronous basements. However, it correlates well with heat flow anomalies. The boundary area is confined to the Baltic coast [Sim, 2000. Along the boundary area, near the Baltic Sea, there is an area wherein faulting is mainly caused by extension [Sim, 2000. In this setting, helium permeability is the highest, as shown by the crust map of the European part of the USSR [Eremeev, 1983. Extension in this area is probably related to formation of young grabens in the Baltic shield. Changes in the compression axis orientation may be due to the alternating activations of the grabens in the submeridionalBotnicGulf and the latitudinalGulf of Finland. Reconstructions for individual faults show contradictions in the directions of shear displacements: both right- and left-lateral displacements are possible on the same fault segments, and the axes of compression can have either latitudinal or meridional orientations. The focal mechanisms of the Osmussaar andKaliningrad earthquakes (meridional and latitudinal axes of compression, respectively) give evidence of specific current neotectonic stresses in this area. Another zone is distinguished at 52°N from the above-described area. It is mainly sublatitudinal and detected along the southern flank of the Byelorussian anteclise. Further to the east, its orientation changes to SSW, and it roughly follows the SW boundary of theVoronezh anteclise. Reconstructions for the Ukrainian Shield, located south of this zone, show mainly the unstable orientations of the axes of compression. For the platforms inNorthern Eurasia, the tectonophysical methods reconstructed neotectonic stresses in the structures formed under the influence of intraplatform tectonic stresses. These are the residual gravitational horizontal compression stresses released by long-term denudation and uplifting of the structures, including the Khibiny massif of the Baltic Shield, theOlenek and Munsky massifs of the East Siberian platform. These structures are composed of the ancient Archaean-Proterozoic rock complexes, which have been subjected to predominantly vertical displacements for a long time, from the Paleozoic to the modern stage. Special attention should be given to the tectonic stresses ofSakhalin located at the boundary between the Eurasian and North American lithospheric plates. At the edges of these two largest plates, there are the Amur and Okhotsk microplates separated by theCentral Sakhalin fault, as described in some publications. Neotectonic stress reconstructions forSakhalinIsland show sublatitudinal compression and submeridional extension in the common stress field of shearing. The tectonophysical studies show that the neotectonic stresses differ in large structures: horizontal compression and shearing are typical of the uplifts (Kola Peninsula, Tien Shan, Sakhalin), while horizontal extension and extension with shearing are characteristic of depressions (Kandalaksha graben, depressions of theTatarGulf and theSea ofOkhotsk). Our studies provide the data on spacious ‘white spots’ in the modern stress maps ofNorthern Eurasia. The stress reconstructions for practically all the studied structures show that shearing is the dominant geodynamic regime in the study region.

The article substantiates the urgent need for the formation of import-substituting clusters in the agro-industrial complex to ensure food security. In modern reality, carrying out business activities, agro-industrial organizations are already using the advantages of integration to a certain extent, but at the same time, the key interests and guidelines of allied participants in entrepreneurial activities are insufficiently balanced. The authors have identified the methodological features of the formation of the subject of integration (agri-food polycluster formation), using the cluster tools of intersectoral reengineering. The basis for the introduction of the subjects of integration - allied participants in entrepreneurial activity - was their initial assessment and analysis of the factors and conditions of business activity, where the negative results of import substitution, which is an effective threat to food security, can be traced as much as possible. Evaluation of the target pairing through the integration of related business participants allows one to quantitatively assess the predominance of the target points of integration subjects - related business participants using information at the macro and micro levels.

The paper describes the neotectonics of the Sakhalin Island and analyzes the latest and recent tectonic stresses in the study area in order to establish their differences in the Amur and Okhotsk microplates, which boundary is confined to the Tym-Poronaisk fault, the largest NS-striking fault in the Central Sakhalin (Fig. 1). Our map of the structural geomorphological features of the study area (Fig. 2) shows three longitudinal zones: the western and eastern uplifts, and the Central Sakhalin basin between the uplifts. In the Southern Sakhalin, neotectonic stresses were studied by a combination of tectonophysical methods and the method of structural geology (Figures 3 to 6, and Table). Our study shows that the regional axes of maximum and minimum compressive principal normal stresses are primarily of the subhorizontal orientations (Fig. 5, Д). In the Northern and Central Sakhalin, neotectonic stresses were reconstructed by the structural geomorphology method. The compression axes are oriented sublatitudinally, with the NE-trending strike in the Northern Sakhalin (Fig. 7, A), and the extension axes are oriented submeridionally; in the Northern Sakhalin, respectively, they are oriented in the NW direction. The results of our study of neotectonic stresses were used to construct a map of recent geodynamics of Sakhalin (Fig. 7, Б), which shows zones differing in the geodynamic settings of the most recent faulting. According to the analysis of the recent tectonic stress with respect to the earthquake focal mechanisms in the period from 1978 to 2015 (Fig. 8), recent stresses dominating in Sakhalin have mainly the sublatitudinal low-angle orientations of the deviatoric compression axis. The submeridional low-angle orientations of the deviatoric extension axes are observed in the Northern Sakhalin and partly in the north of the Southern Sakhalin (see Fig. 8). The high-angle axes of deviatoric extension are typical of the western and central parts of the Southern Sakhalin, and such extension leads to horizontal compression and reverse faulting. In some areas of the recent stress field, the deviatoric axes of compression and extension have unstable orientations. The latitudinal boundaries of such areas are nearly coincident with the boundaries of the zones that differ in the geodynamic settings of the most recent faulting, which means that these areas and zones are reliably identified. The relative inhomogeneity of the neotectonic and recent stress fields in the Southern Sakhalin does not give grounds to distinguish differences in the state of crustal stresses in the areas located on the sides of the Southern Sakhalin fault. As a consequence, a boundary between the Amur and the Okhotsk Plate in the South Sakhalin cannot be drawn along this fault. It is most likely that this boundary coincides with the Western-Sakhalin fault in the southern areas of the study region. Our data on the Central and Northern Sakhalin does not contradict with the conclusion in [Savostin et al., 1982] concerning this boundary.

The secondary structure of horse cytochrome c with mutations in the P76GTKMIFA83 site of the Ω-loop, exhibiting reduced efficiency of electron transfer, were studied. CD spectroscopy studies showed that the ordering of mutant structure increases by 3–6% compared to that of the WT molecules due to the higher content of β-structural elements. The IR spectroscopy data are consistent with the CD results and demonstrate that some α-helical elements change into β-structures, and the amount of the non-structured elements is decreased. The analysis of the 1H-NMR spectra demonstrated that cytochrome c mutants have a well-determined secondary structure with some specific features related to changes in the heme microenvironment. The observed changes in the structure of cytochrome c mutants are likely to be responsible for the decrease in the conformational mobility of the P76GTKMIFA83 sequence carrying mutations and for the decline in succinate:cytochrome c-reductase and cytochrome c-oxidase activities in the mitoplast system in the presence of these cytochromes c. We suggest that the decreased efficiency of the electron transfer of the studied cytochromes c may arise due to: (1) the change in the protein conformation in sites responsible for the interaction of cytochrome c with complexes III and IV and (2) the change in the heme conformation that deteriorates its optimal orientation towards donor and acceptor in complexes III and IV therefore slows down electron transfer. The results obtained are consistent with the previously proposed model of mitochondrial cytochrome c functioning associated with the deterministic mobility of protein globule parts.

Purpose: We evaluate various approaches to target volume definition and boost delivery in patients with complete response to neoadjuvant systemic therapy (NST) who were treated by radiotherapy without a surgery. Materials and Methods: A pathological complete response (pCR) was diagnosed in 21 of 27 patients included in "surgery de-escalation" prospective observation study. Clips were placed in the primary tumor volume (PrTV) before NST and during the vacuum aspiration biopsy. Twenty patients with pCR underwent the whole breast irradiation and a boost to the PrTV. High-dose rate brachytherapy (HDRB) was the basic technique for boost delivery. Finally, we identified the value of fused images (computed tomography [CT] before NST with simulation CT), clips and their combination for an accurate boost delivery. Results: A complete overlap between PrTV on pre-treatment CT with the localization of the clips on simulation CT was mentioned in 10, partial mismatch in three patients. In 12 of these 13 women, HDRB was successfully used for the boost delivery. In five cases we mentioned a marked discrepancy between the PrTV on fused images and the topography of the clips. In other two women we did not find clips on simulation CT. The fused images in five of these seven patients showed anatomical landmarks (scar, fibrosis) used for identification of the gross tumor volume. In all 20 women with pCR (average follow-up of 16.6 months), there were no locoregional recurrences. Conclusion: Combination of the clips with fusion of pre-NST and simulation CTs is important for an accurate boost delivery. [ABSTRACT FROM AUTHOR]

The article deals with the problem of accumulation and usage oftechnogenic metallurgical resources in the regions where the largeststeel plants are accommodated. The features of exploration anddevelopment of the mineral potential of technogenic metallurgicalresources are presented through the introduction of technologies ofdeep complex processing of technogenic raw materials. The topicalityof technogenic raw materials usage to expand the mineral resource baseof zinc producers in Russia is substantiated. The prospects of thezinc industry in terms of development of raw conversion by usingtechnological resources are explored. A methodical approach toevaluating the effectiveness of the usage of technogenic metallurgicalraw materials is developed. Approaches to establish the price oftechnogenic raw materials are determined; the expediency of increasingenvironmental charges for waste disposal is highlighted.Methodological developments are tested to assess the effectiveness of the usage of technogenic waste products made by ferrous metallurgyplants in Russia as a raw material for zinc production. There are set of the prioritiesfor the usage of raw potential of the technogenic resources todevelop the zinc industry.

In the article, the key stage of the value chain in the zinc industry of the Russian Federation is considered. The study aims to examine the impact of technological change on the development of value chains in the mining and metallurgical complex in the Urals region. The relevance of the assessment of the impact of technological change on the development of value chains in the processing of primary and technogenic raw materials in terms of the Ural Mining and Metallurgical Complex is grounded. The methodological scheme of analysis of value chains and the impact on them of new technologies are developed in the study. It is shown that the analysis of value chains should be carried out, using as an analytical tool value added as well as a heuristic tool study of the value chain. Analysis of the value chain in the production of primary zinc and technogenic raw materials made in accordance with the proposed approach. An assessment of the value added in the production of zinc from primary raw materials held in the article. The gross volume and dynamics of revenue, net income and the cost of segments of the value chain in zinc production are analyzed. The leading role of the metallurgical segment in the value chain of the zinc industry is established. Share indexes of the metallurgical segment as a control link of the value chain in the structure of the consolidated revenue, profit, cost considered in the dynamics, the dynamics of change in the total expenditure on scientific and technological development and R & D expenditure are investigated. The conclusion about essential influence of new technologies on the change of value chains in terms of zinc production is confirmed.

Tatyana Y Kuznetsova,1 Viktoria A Korneva,1 Evgeniya N Bryantseva,2 Vitaliy S Barkan,2 Artemy V Orlov,3 Igor N Posokhov,4 Anatoly N Rogoza51Faculty of Medicine, Petrozavodsk State University, Petrozavodsk, Russia; 2Diagnostics Department, The hospital within the Russian Railroad Network, Chita, Russia; 3Department 65 Competitive System Analysis, National Research Nuclear University MEPhI, Moscow, Russia; 4Hemodynamic Laboratory Ltd, Nizhniy Novgorod, Russia; 5Cardiology Research Center, Moscow, RussiaAbstract: The purpose of this study was to examine the pulse wave velocity, aortic augmentation index corrected for heart rate 75 (AIx@75), and central systolic and diastolic blood pressure during 24-hour monitoring in normotensive volunteers. Overall, 467 subjects (206 men and 261 women) were recruited in this study. Participants were excluded from the study if they were less than 19 years of age, had blood test abnormalities, had a body mass index greater than 27.5 kg/m2, had impaired glucose tolerance, or had hypotension or hypertension. Ambulatory blood pressure monitoring (ABPM) with the BPLab® device was performed in each subject. ABPM waveforms were analyzed using the special automatic Vasotens® algorithm, which allows the calculation of pulse wave velocity, AIx@75, central systolic and diastolic blood pressure for “24-hour”, “awake”, and “asleep” periods. Circadian rhythms and sex differences in these indexes were identified. Pending further validation in prospective outcome-based studies, our data may be used as preliminary diagnostic values for the BPLab ABPM additional index in adult subjects.Keywords: ambulatory, monitoring, arterial stiffness, augmentation index, central blood pressure, reference values

In the article, the perspective trends of the expansion of the mineral-ore base of the Russian producers of zinc by recycling of technogenic raw materials are considered. The important role of recycling of resources for sustainable development of society and improve the environmental safety is justified. The main structural and dynamic characteristics of the use of mineral resource base for the production of zinc in Russia are considered. Raw materials opportunities and constraints for the development of zinc production are analyzed. In the paper, the structure and dynamics of the use of recycled materials by the largest producer of zinc in Russia are investigated. The methodical approach to the estimation of effectiveness of the industrial processing of technogenic metallurgical raw materials with the strategic flexibility of the implementation of projects is proposed and approved. The estimation of the effectiveness of a complex industrial processing of metallurgical zinc-containing dusts in a real production is carried out. The value of the strategic flexibility of the project of the industrial processing of the zinc-containing technogenic raw material is determined on the basis of the developed systematic approach. The value of the processes of recycling for sustainable production of zinc in Russia is revealed.

The article analyzes the common mistakes that allow educators and teachers in the design of the visual component of computer slide presentations to lessons. Detail the most common technological and conceptual errors: the use of photographic images of low resolution, "distortion" of the image on the slide, abuse banal images, the image on the slide, not "correlated" with the educational context. Methodical recommendations to address the negative impact of technological and conceptual errors in the visual perception of the slide.