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Soft materials, such as soft biological tissue and soft silicone rubber, are non-linear materials which require the classical uniaxial and biaxial tensile testing methods for characterisation. Unfortunately, in special cases, such as for soft biological tissue, the samples are smaller than 10 mm × 10 mm in size and these classical tensile testing methods produce unwanted stress and strain gradients due to the fastening techniques associated with these methods. Micro-indentation is proposed as an alternative method for characterising soft materials. Using inverse Finite Element (FE) analysis and a known Mooney-Rivlin three parameter material model, six different micro-indentation tests were proposed. A theoretical approach was used to determine which indentation test best characterised a silicone sample, by using two FE models. The results showed that microindentation is capable of characterising a soft material in ideal conditions with a cylindrical indenter applied in a diagonal orientation over the sample, as the best indentation method. Finally, it was observed that the material model can either match the displacements with the smallest objective function or the stress vs. stretch curve can be matched to 99 % over the whole stretch range but not both simultaneously. [ABSTRACT FROM AUTHOR]

The incorporation of perforated cortical bone allografts was compared with non-perforated allografts. A 5-cm circumferential defect in the middiaphysis at the tibia was created in adult sheep. A frozen tibial allograft was implanted and fixed with a locked nail for 6 months. There was no postoperative immobilization. Group I consisted of eight sheep with non-perforated allografts, whereas Group II was comprised of 10 sheep with perforated allografts. Union was evaluated radiographically, whereas the central part of the allograft had a densitometric evaluation. Creeping substitution was assessed on microradiographs from cross-sections of the central 3 cm of graft by measurement of porosity and percentage of new and old bone area within the confines of the graft. The width of periosteal and endosteal callus also was determined. There was no statistical difference between both groups for the union score and bone density. However, the cortical bone graft porosity and the amount of new bone within the cortical bone differed significantly between the perforated allografts and the non-perforated ones. Periosteal callus was similar in both groups, whereas endosteal callus was significantly more wide and extended in the perforated allografts. Perforation of a cortical bone substantially improved the amount of newly formed bone by the host when compared with a non-perforated bone. The creation of channels seemed to increase the interface between living soft tissues of the host and the allografted bone with a resulting enhanced incorporation.

We compared the reliability of computer-assisted radiographic analysis (CARA) and visual evaluation of radiographs to assess host-graft junctions. 68 host bone/allograft junctions were obtained from an ongoing study on bone allografting in sheep. At 6 months, the grafted tibias were explanted and healing of the host-graft junctions were macroscopically determined. 49 junctions were macroscopically healed, whereas 19 had not united. 51 (0.8) of the junctions were correctly classified by radiographs, while 63 (0.9) of the junctions were correctly classified by CARA (p = 0.03). These findings warrant further evaluation in a clinical setting.