Search

Abstract Background Aphid adaptation to harsh winter conditions is illustrated by an alternation of their reproductive mode. Aphids detect photoperiod shortening by sensing the length of the night and switch from viviparous parthenogenesis in spring and summer, to oviparous sexual reproduction in autumn. The photoperiodic signal is transduced from the head to the reproductive tract to change the fate of the future oocytes from mitotic diploid embryogenesis to haploid formation of gametes. This process takes place in three consecutive generations due to viviparous parthenogenesis. To understand the molecular basis of the switch in the reproductive mode, transcriptomic and proteomic approaches were used to detect significantly regulated transcripts and polypeptides in the heads of the pea aphid Acyrthosiphon pisum. Results The transcriptomic profiles of the heads of the first generation were slightly affected by photoperiod shortening. This suggests that trans-generation signalling between the grand-mothers and the viviparous embryos they contain is not essential. By analogy, many of the genes and some of the proteins regulated in the heads of the second generation are implicated in visual functions, photoreception and cuticle structure. The modification of the cuticle could be accompanied by a down-regulation of the N-β-alanyldopamine pathway and desclerotization. In Drosophila, modification of the insulin pathway could cause a decrease of juvenile hormones in short-day reared aphids. Conclusion This work led to the construction of hypotheses for photoperiodic regulation of the switch of the reproductive mode in aphids.

We present a design of a pillared phononic crystal based structure for Love wave manipulation to achieve high mass sensitivity. The structure is made of phononic micro-pillars constructed by stacking tungsten and SiO2 layers, distributed on a substrate designed for Love wave propagation. The multilayered pillar allows the creation of bandgaps, which leads to the existence of resonant modes where the elastic energy is confined within the SiO2 free surface layer of the pillar. We study particularly a resonant mode where this layer exhibits torsional mechanical motion which can only be excited by shear horizontal surface waves. We show that Love wave interaction with the torsional mode gives rise to a sharp attenuation in the surface wave transmission spectrum with a high quality factor. We also study the variation of the mass sensitivity of the system by evaluating the resonant mode's frequency shift induced by a mass perturbation using two theoretical approaches: a perturbation theory based approximation and a numerical method. The system presents very promising mass sensitivity which provides an interesting approach to increase the detection performance of Love wave based bio-sensors. [ABSTRACT FROM AUTHOR]

In this paper, the phenomenon of delamination under static and dynamic loading of a composite made of an epoxy matrix and carbon fiber reinforcement has been studied, analyzing its fracture behavior under mixed mode I/II loading employing an asymmetric double cantilever beam test. Under static loading, some of the most representative formulations for calculating the energy release rate were analyzed, finding a good agreement between the results obtained by means of the different formulations. Under dynamic loading, the number of cycles necessary for the crack onset was determined (determination of ΔG − N fatigue curves and number of cycles necessary for the delamination onset for a given energy release rate). As regards the experimental results, apparent fatigue limits of the order of 38% of the critical fracture energy were obtained for an asymmetry coefficient of 0.1. Subsequent statistical analysis of the results enabled the fatigue limit to be defined more accurately. This was found to be 15% for this material, indicating the need to use these tools for the actual determination of the infinite fatigue life of the material. Finally, an optical study of the fracture surfaces was carried out which confirmed the presence of mixed mode fracture typologies. [ABSTRACT FROM AUTHOR]

Cryptococcosis is a major concern in organ transplant recipients. A decrease in immunosuppressants following the initiation of antifungal therapy is currently recommended, but can occasionally be complicated by the onset of immune reconstitution inflammatory syndrome ( IRIS). We report on a case of cryptococcosis in a kidney transplant recipient, compounded by severe neurological IRIS, the outcome of which was unfavorable despite the use of anti-tumor necrosis factor-alpha monoclonal antibodies. [ABSTRACT FROM AUTHOR]

The characterization of the rolling resistance is an important issue when designing mechanical systems in order to predict the force needed to run a given mechanism. Frictional forces in bearings and rolling resistance in rollers and wheels are the most important factors contributing to the overall resistance to the movement. As the rolling coefficient varies as a function of the material nature, normal load, and rolling velocity, it is desirable to test the designed system under real conditions. Mechanical testing machines, present in most mechanical laboratory tests, usually work in uniaxial mode. In this work a simple testing device to be used with uniaxial mechanical testing machines is presented. [ABSTRACT FROM AUTHOR]

In this paper, a modified mechanical grip fitting is used to perform double cantilever beam (DCB) tests. The advantage of using mechanical grips instead of piano hinges or end blocks lies in the fact that the use of adhesive bonding is not required to fix the sample. Adhesive bonding can be an important source of uncertainty and unexpected debondings in fatigue tests. Mechanical fittings are also well suited for high temperature applications where adhesive bonds usually undergo premature failure. An experimental programme has been developed in order to compare the performance of the modified mechanical hinges with classical hinges and end blocks. The highest G Ic values and scattering were found for the end block system, while hinges and mechanical hinges furnished similar results. The material used to perform the experimental study was a Hexcel AS4/3501-6 unidirectional laminate. [ABSTRACT FROM AUTHOR]

In this work, the fracture behavior under modes I, II, and mixed mode I/II has been studied for two different AS4 carbon fiber epoxy laminates. One of the laminates was produced with a Hexcel 3501-6 epoxy resin while the other was laminated with a tougher modified Hexcel 8552 epoxy resin. Both laminates were experimentally tested in modes I, II, and mixed I/II with different mixity ratios by means of DCB (double cantilever beam), ENF (end notch flexure), and MMB (mixed mode bending) specimens, respectively. Finite element modeling (FEM) was used in order to analyze modes I, II, and mixed I/II and to compare experimental and numerical results. The modified 8552 resin matrix presented the best behavior in mode I and mixed mode I/II as the critical energy release rate was higher than that for the 3501-6 matrix composites. In mode II, the best performance was reached for the 3501-6 matrix laminates. It was also found that the critical energyGcand the scatter increased as the mode ratioGII/Gcincreased. Finally, experimental and numerical results showed a good agreement as the differences obtained from both procedures were generally lower than 10%. [ABSTRACT FROM PUBLISHER]

Abstract: In this paper unidirectional carbon epoxy End Notch Flexure samples (ENF) with different crack plane positions have been analyzed in order to study the influence of the degree of asymmetry over the load state at the crack tip. This study has been performed from different points of view: experimental, numerical approaches (Finite Element Models) and fractographic observations. From these studies it has been concluded that the deviation of the crack plane from the specimen midplane has no influence or little influence over the corresponding critical value of the energy release rate in mode II (G II ). In other words, the asymmetry of the crack plane does not induce mode I load state at the crack tip while the energy release rate related to the mode II load remains almost constant as the crack plane moves from the midplane. Fractographic observations have shown “hackle markings” structures typical from mode II fracture. Finally, a good agreement was found between experimental and numerical procedures as errors were always lower than 7%. [Copyright &y& Elsevier]

In this study, the delamination fatigue behavior of two different aeronautical quality composites under mode-II notched flexure loading has been experimentally investigated with the aim of analyzing the effect of the resin type used. Both composites contain the same unidirectional AS4 carbon reinforcement, though embedded in two different matrices: an 8552 epoxy with improved toughness and an unmodified brittle 3501-6 epoxy. The end notched flexure test was found suitable for promoting fatigue delamination under mode-II loading. First, the ΔG-N fatigue curves were determined as the number of cycles necessary for crack onset at a given energy release rate. Then the crack growth rate was obtained for different fractions of the critical energy release rates, Gcr. Calculations of the shear toughness were carried out in accordance with the methods proposed in the ESIS protocol. Critical experimental details are reviewed and the influence of the different procedures proposed for the data assessment of results is analyzed. The results confirm, on the one hand, the influence of the manufacturing process on fatigue crack initiation, and, on the other, the improved performance of the modified resin, both in terms of crack initiation and growth rate. [ABSTRACT FROM PUBLISHER]

This work deals with the determination of the critical strain energy release rate ([image omitted] by means of experimental and numerical methods in unidirectional carbon epoxy composite laminates in modes I and II, and the influence of the test configuration compliance on the results. In previous works, it was found that the determination of GIc by means of experimental procedures and numerical determination using the Finite Element Method (FEM), presented differences in the order of 20-30%. In order to improve the convergence of both numerical and experimental models, research was carried out about the points that could have influenced the results, i.e., FEM element type and size, material behavior law and testing compliance. From this research it was demonstrated that the testing machine compliance had a great influence over the obtained results. The introduction of a correction for testing machine compliance in the calculation has lowered the difference between numerical and experimental results from 20-30% to less than 10% in both modes I and II. [ABSTRACT FROM AUTHOR]