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1. Identification of metabolic and protein markers representative of the impact of mild nitrogen deficit on agronomic performance of maize hybrids

Author

Urrutia, Maria, Blein-Nicolas, Mélisande, Fernandez, Olivier, Bernillon, Stéphane, Maucourt, Mickaël, Deborde, Catherine, Balliau, Thierry, Rabier, Dominique, Bénard, Camille, Prigent, Sylvain, Quilleré, Isabelle, Jacob, Daniel, Gibon, Yves, Zivy, Michel, Giauffret, Catherine, Hirel, Bertrand, and Moing, Annick

Published
2024
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5. Ecological and metabolic implications of the nurse effect of Maihueniopsis camachoi in the Atacama Desert.

Author

Díaz, Francisca P., Dussarrat, Thomas, Carrasco‐Puga, Gabriela, Colombié, Sophie, Prigent, Sylvain, Decros, Guillaume, Bernillon, Stéphane, Cassan, Cédric, Flandin, Amélie, Guerrero, Pablo C., Gibon, Yves, Rolin, Dominique, Cavieres, Lohengrin A., Pétriacq, Pierre, Latorre, Claudio, and Gutiérrez, Rodrigo A.

Subjects
DESERTS, PHYTOGEOGRAPHY, TEMPERATURE distribution, BIOMARKERS, MACHINE learning
Abstract

Summary: Plant–plant positive interactions are key drivers of community structure. Yet, the underlying molecular mechanisms of facilitation processes remain unexplored. We investigated the 'nursing' effect of Maihueniopsis camachoi, a cactus that thrives in the Atacama Desert between c. 2800 and 3800 m above sea level. We hypothesised that an important protective factor is thermal amelioration of less cold‐tolerant species with a corresponding impact on molecular phenotypes.To test this hypothesis, we compared plant cover and temperatures within the cactus foliage with open areas and modelled the effect of temperatures on plant distribution. We combined eco‐metabolomics and machine learning to test the molecular consequences of this association.Multiple species benefited from the interaction with M. camachoi. A conspicuous example was the extended distribution of Atriplex imbricata to colder elevations in association with M. camachoi (400 m higher as compared to plants in open areas). Metabolomics identified 93 biochemical markers predicting the interaction status of A. imbricata with 79% accuracy, independently of year.These findings place M. camachoi as a key species in Atacama plant communities, driving local biodiversity with an impact on molecular phenotypes of nursed species. Our results support the stress‐gradient hypothesis and provide pioneer insights into the metabolic consequences of facilitation. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Leaf metabolomic data of eight sunflower lines and their sixteen hybrids under water deficit☆

Author

Berton Thierry, Bernillon Stéphane, Fernandez Olivier, Duruflé Harold, Flandin Amélie, Cassan Cédric, Jacob Daniel, Langlade Nicolas B., Gibon Yves, and Moing Annick

Subjects
helianthus, abiotic stress, drought stress, lc-ms, metabolomic profiling, Oils, fats, and waxes, TP670-699
Abstract

This article describes how metabolomic data were produced on sunflower plants subjected to water deficit. Twenty-four sunflower (Helianthus annuus L.) genotypes were selected to represent genetic diversity within cultivated sunflower and included both inbred lines and their hybrids. Drought stress was applied at the vegetative stage to plants cultivated in pots using the high-throughput phenotyping facility Heliaphen. Here, we provide untargeted and targeted metabolomic data of sunflower leaves. These compositional data differentiate both plant water status and different genotype groups. They constitute a valuable resource for the community to study the adaptation of crops to drought and the metabolic bases of heterosis.

Published
2021
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16. Extensive metabolic cross-talk in melon fruit revealed by spatial and developmental combinatorial metabolomics

Author

Moing, Annick, Aharoni, Asaph, Biais, Benoit, Rogachev, Ilana, Meir, Sagit, Brodsky, Leonid, Allwood, J. William, Erban, Alexander, Dunn, Warwick B., Kay, Lorraine, de Koning, Sjaak, de Vos, Ric C. H., Jonker, Harry, Mumm, Roland, Deborde, Catherine, Maucourt, Michael, Bernillon, Stéphane, Gibon, Yves, Hansen, Thomas H., Husted, Søren, Goodacre, Royston, Kopka, Joachim, Schjoerring, Jan K., Rolin, Dominique, and Hall, Robert D.

Published
2011

17. Combining phenotypic, metabolome and proteome data to study maize response to a mild nitrogen deficit

Author

Urrutia, Maria, Blein-Nicolas, Melisande, Fernandez, Olivier, Bernillon, Stéphane, Maucourt, Mickael, Deborde, Catherine, Balliau, Thierry, Bénard, Camille, Prigent, Sylvain, Quilleré, Isabelle, Jacob, Daniel, Gibon, Yves, Zivy, Michel, Giauffret, Catherine, Hirel, Bertrand, Moing, Annick, and Blein-Nicolas, Mélisande

Subjects
[SDV] Life Sciences [q-bio], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding
Abstract

To decipher the biochemical bases of nitrogen (N) utilization and metabolism of silage maize inrelation to growth and productivity, an untargeted metabolomic (proton NMR- and LC-QTOF-MSbased) and proteomic approach was conducted on leaves of 29 hybrids cultivated in the field underoptimal and reduced N fertilization. The corresponding biochemical data were analyzed eitherindividually (PCA, ANOVA), or integrated with that of eco-physiological, developmental and yield-related traits (multi-block sparse PLS-DA). Such integrated analysis was conducted to interpret theunderlying physiology concerning the plant response to a mild N deficit often occurring underagronomic conditions. The genetic diversity of the core panel of 29 European dent hybrids crossed toa flint tester was exploited to highlight common N-responsive metabolites and proteins in order toidentify putative biological markers that could be used to pilot and rationalize N fertilization. Theresponses of metabolites, proteins, and yield-related traits to the reduced N treatment were alsoexploited to identify biochemical markers representative of a maize ideotype exhibiting betteragronomic performances when N fertilization is limited. These markers could be used to select high-yielding commercial maize hybrids used for silage production requiring less N fertilizer inputs.

Published
2022

18. Presence/absence variations and SNPs equally contribute to the variations of protein and metabolite abundance

Author

Djabali, Yacine, Blein-Nicolas, Melisande, Moing, Annick, Duarte, Jorge, Bernillon, Stéphane, Prigent, Sylvain, Mabire, Clément, Madur, Delphine, Cabrera-Bosquet, Llorenç, Welcker, Claude, Tardieu, Francois, Gibon, Yves, Zivy, Michel, Charcosset, Alain, Nicolas, Stephane, and Blein-Nicolas, Mélisande

Subjects
[SDV] Life Sciences [q-bio], [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding
Abstract

Understanding the mechanisms of adaptation to the environment in cultivated plants is a promising way to meet the challenge of maintaining food security in the context of global warming. In the case of maize, high-throughput sequencing has revealed that structural variations represent a large part of the genome and could have huge phenotypic effects. Among these, Presence Absence Variants (PAVs, which include insertion/deletion of large DNA sequences) may be involved in adaptation of maize to its environment, but their contribution to the genetic determinism of traits and genotype by environment interactions remains largely unknown. To address this issue, we performed a genome-wide association study between two types of polymorphisms, SNPs and InDels, and molecular traits obtained from proteomics and metabolomics analyses to detect quantitative trait loci (QTLs). The genetic panel used for this study was composed of 254 dent inbred lines genotyped with 978,134 SNPs and 72,041 InDels. The latter encompassed from 37 to 129,700 pb, including thousands of PAVs that are not present in the B73 reference genome. Proteins and metabolites were quantified by mass spectrometry in leaf samples from F1 hybrids obtained by crossing the inbred lines with one flint tester line. Hybrid plants were grown under two watering conditions (well-watered and water deficit) in greenhouse. In total, we detected 61,225 QTLs associated with proteome or metabolome variations. Among these, 4,766 QTLs were exclusively detected by InDels. To take into account the difference of marker density between InDels and SNPs, we used a re-sampling approach which showed that there was no difference between InDels and SNPs regarding the number and effect of the QTLs detected . Additionally, the QTLs detected by the two types of polymorphism were equally distributed in the two watering conditions. These preliminary results show that InDels are worse considering to detect new genetic regions of interest. They also suggest that InDels and SNPs equally contribute to molecular trait variation and response to drought stress.

Published
2022

20. Presence/absence variations and SNPs equally contribute to the variations of protein and metabolite abundance

Author

Djabali, Yacine, Blein, Mélisande, Moing, Annick, Duarte, Jorge, Berton, Thierry, Bernillon, Stéphane, Prigent, Sylvain, Fernandez, Olivier, Pétriacq, Pierre, Mabire, Clément, Madur, Delphine, Cabrera-Bosquet, Llorenç, Welcker, Claude, Tardieu, Francois, Gibon, Yves, Zivy, Michel, Charcosset, Alain, Nicolas, Stephane, Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), AgroParisTech-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Metabolome Bordeaux, INRAE, 2018, MetaboHUB, Centre INRAE Nouvelle Aquitaine Bordeaux, doi: 10.15454/1.5572412770331912E12 (PMB), Institut National de la Recherche Agronomique (INRA), Groupe Limagrain, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ANR-10-BTBR-0001,AMAIZING,Développer de nouvelles variétés de maïs pour une agriculture durable: une approche intégrée de la génomique à la sélection(2010)

Subjects
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], food and beverages
Abstract

International audience; Understanding the mechanisms of adaptation to the environment in cultivated plants is a promising way to meet the challenge of maintaining food security in the context of global warming. In the case of maize, high-throughput sequencing has revealed that structural variations represent a large part of the genome and could have huge phenotypic effects. Among these, Presence Absence Variants (PAVs) may be involved in adaptation of maize to its environment, but their contribution to the genetic determinism of traits and genotype by environment interactions remains largely unknown. To address this issue, we performed a genome-wide association study between two types of polymorphisms, SNPs and Insetions/Deletions (InDels), and molecular traits obtained from proteomics and metabolomics analyses. The genetic panel used for this study was composed of 254 dent inbred lines genotyped with 978,134 SNPs and 72,041 InDels. The latter encompassed from 37 to 129,700 pb, including thousands of PAVs that are not present in the B73 reference genome. Proteins and metabolites were quantified by mass spectrometry in leaf samples from F1 hybrids obtained by crossing the inbred lines with one flint tester line. Hybrid plants were grown under two watering conditions (well-watered and water deficit) in greenhouse. In total, we detected 61,225 QTLs associated with proteome or metabolome variations. Among these, 4,766 QTLs were exclusively detected by InDels. To take into account the difference of marker density between InDels and SNPs, we used a re-sampling approach which showed that there is no difference for effect size distribution of QTLs between InDels and SNPs and for the number of QTLs detected by InDels or SNPs. Additionally, the QTLs detected by the two types of polymorphism were equally distributed in the two watering conditions. Our results suggest that InDels and SNPs equally contributed to molecular trait variation and response to drought stress.

Published
2021

23. Plant Nitrate Reductases Regulate Nitric Oxide Production and Nitrogen-Fixing Metabolism During the Medicago truncatula–Sinorhizobium meliloti Symbiosis

Author

BERGER, Antoine, BOSCARI, Alexandre, HORTA ARAÚJO, Natasha, MAUCOURT, Mickaël, HANCHI, Mohamed, BERNILLON, Stéphane, ROLIN, Dominique, PUPPO, Alain, BROUQUISSE, Renaud, Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), University of Florida [Gainesville] (UF), Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Platetorme Métabolome Bordeaux, Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche en Agronomie, Centre National de la Recherche Scientifique (CNRS), Côte d'Azur University, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), and ANR-15-CE20-0005,STAYPINK,Mécanismes contrôlant la transition entre fixation d'azote et sénescence dans les nodosités symbiotiques de légumineuses(2015)

Subjects
nitrogen-fixing symbiosis, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, nitrate reductase, Métabolisme, hypoxia, nitric oxide, legumes, [SDV]Life Sciences [q-bio], Medicago truncatula, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, nodules
Abstract

Nitrate reductase (NR) is the first enzyme of the nitrogen reduction pathway in plants, leading to the production of ammonia. However, in the nitrogen-fixing symbiosis between legumes and rhizobia, atmospheric nitrogen (N 2) is directly reduced to ammonia by the bacterial nitrogenase, which questions the role of NR in symbiosis. Next to that, NR is the best-characterized source of nitric oxide (NO) in plants, and NO is known to be produced during the symbiosis. In the present study, we first surveyed the three NR genes (MtNR1, MtNR2, and MtNR3) present in the Medicago truncatula genome and addressed their expression, activity, and potential involvement in NO production during the symbiosis between M. truncatula and Sinorhizobium meliloti. Our results show that MtNR1 and MtNR2 gene expression and activity are correlated with NO production throughout the symbiotic process and that MtNR1 is particularly involved in NO production in mature nodules. Moreover, NRs are involved together with the mitochondrial electron transfer chain in NO production throughout the symbiotic process and energy regeneration in N 2-fixing nodules. Using an in vivo NMR spectrometric approach, we show that, in mature nodules, NRs participate also in the regulation of energy state, cytosolic pH, carbon and nitrogen metabolism under both normoxia and hypoxia. These data point to the importance of NR activity for the N 2-fixing symbiosis and provide a first explanation of its role in this process.

Published
2020
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24. Predictive metabolomics of multiple Atacama plant species unveils a core set of generic metabolites for extreme climate resilience.

Author

Dussarrat, Thomas, Prigent, Sylvain, Latorre, Claudio, Bernillon, Stéphane, Flandin, Amélie, Díaz, Francisca P., Cassan, Cédric, Van Delft, Pierre, Jacob, Daniel, Varala, Kranthi, Joubes, Jérôme, Gibon, Yves, Rolin, Dominique, Gutiérrez, Rodrigo A., and Pétriacq, Pierre

Subjects
CLIMATE extremes, PLANT species, METABOLITES, PLANT adaptation, SPECIES specificity, CROP quality
Abstract

Summary: Current crop yield of the best ideotypes is stagnating and threatened by climate change. In this scenario, understanding wild plant adaptations in extreme ecosystems offers an opportunity to learn about new mechanisms for resilience. Previous studies have shown species specificity for metabolites involved in plant adaptation to harsh environments.Here, we combined multispecies ecological metabolomics and machine learning‐based generalized linear model predictions to link the metabolome to the plant environment in a set of 24 species belonging to 14 families growing along an altitudinal gradient in the Atacama Desert.Thirty‐nine common compounds predicted the plant environment with 79% accuracy, thus establishing the plant metabolome as an excellent integrative predictor of environmental fluctuations. These metabolites were independent of the species and validated both statistically and biologically using an independent dataset from a different sampling year. Thereafter, using multiblock predictive regressions, metabolites were linked to climatic and edaphic stressors such as freezing temperature, water deficit and high solar irradiance.These findings indicate that plants from different evolutionary trajectories use a generic metabolic toolkit to face extreme environments. These core metabolites, also present in agronomic species, provide a unique metabolic goldmine for improving crop performances under abiotic pressure. [ABSTRACT FROM AUTHOR]

Published
2022
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25. Using metabolomic data to predict Maize yield

Author

Prigent, Sylvain, Fernandez, Olivier, Bernillon, Stéphane, Pétriacq, Pierre, Moing, Annick, Berton, Thierry, Cabrera-Bosquet, Llorenç, Millet, Émilie, Welcker, Claude, Tardieu, François, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Metabolome MetaboHUB INRA Nouvelle Aquitaine, Institut National de la Recherche Agronomique (INRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Gauthier, Muriel

Subjects
[SDV] Life Sciences [q-bio], Métabolome, Plante céréalière, Métabolisme, [SDV]Life Sciences [q-bio], Métabolite, Maïs, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, rendement, ComputingMilieux_MISCELLANEOUS, Métabolomique
Abstract

International audience

Published
2019

26. LC-MS and NMR metabolomics study of a tomato (Solanum lycopersicum L.) mutant affected in the fruit pericarp development

Author

Musseau, Constance, Bernillon, Stéphane, Deborde, Catherine, Jacob, Daniel, Jorly, Joana, Gadin, Stéphanie, Moing, Annick, Gevaudant, Frederic, Fernandez, Lucie, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Metabolome Bordeaux, Institut National de la Recherche Agronomique (INRA), and Réseau Francophone de Métabolomique et Fluxomique

Subjects
Tomato Solanum lycopersicum, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, LC MS, Processing, ComputingMilieux_MISCELLANEOUS, NMR, Métabolomique
Abstract

International audience

Published
2019

27. Mass spectral databases for metabolomics: How to build a consistently annotated mass spectral database from pure reference compounds analyzed under electrospray ionization conditions?

Author

Damont, A., Olivier, M. F., Warnet, A., Lyan, Bernard, Pujos-Guillot, Estelle, Jamin, Emilien, Debrauwer, Laurent, Bernillon, Stéphane, Junot, C., Tabet, J. C., Fenaille, F., Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), MetaboHUB, Université Paris Saclay (COmUE), Institut National de la Recherche Agronomique (INRA), Service de Pharmacologie et Immunoanalyse (SPI), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Plateforme d'Exploration du Métabolisme, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Analytical Platform for Metabolomics and Toxicology (MetaToul-AXIOM), Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Institut Parisien de Chimie Moléculaire (IPCM), Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1

Subjects
High-resolution mass spectrometry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Annotation, Mass spectra, Metabolomics, MS databases
Abstract

Epub ahead of print; Nowadays, high-resolution mass spectrometry is widely used for metabolomic studies. Thanks to its high sensitivity, it enables the detection of a large range of metabolites. In metabolomics, the continuous quest for a metabolite identification as complete and accurate as possible has led during the last decade to an ever increasing development of public MS databases (including LC-MS data) concomitantly with bioinformatic tool expansion. To facilitate the annotation process of MS profiles obtained from biological samples, but also to ease data sharing, exchange and exploitation, the standardization and harmonization of the way to describe and annotate mass spectra seemed crucial to us. Indeed, under electrospray (ESI) conditions, a single metabolite does not produce a unique ion corresponding to its protonated or deprotonated form but could lead to a complex mixture of signals. These MS signals result from the existence of different natural isotopologues of the same compound and also to the potential formation of adduct ions, homo and hetero-multimeric ions, fragment ions resulting from "prompt" in-source dissociations. As a joint reflection process within the French Infrastructure for Metabolomics and Fluxomics (MetaboHUB) and with the purpose of developing a robust and exchangeable annotated MS database made from pure reference compounds (chemical standards) analysis, it appeared to us that giving the metabolomics community some clues to standardize and unambiguously annotate each MS feature, was a prerequisite to data entry and further efficient querying of the database. The use of a harmonized notation is also mandatory for inter-laboratory MS data exchange. Additionally, thorough description of the variety of MS signals arising from the analysis of a unique metabolite might provide greater confidence on its annotation.

Published
2019
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28. Leaf metabolite profiling reveals biochemical diversities within wheat genetic resources

Author

Petriacq, Pierre, Flandin, Amélie, Prigent, Sylvain, Bernillon, Stéphane, CASSAN, Cédric, Rolin, Dominique, Rincent, Renaud, Salon, Christophe, Le Gouis, Jacques, Gibon, Yves, Moing, Annick, Bernard, Nathalie, Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - - METABOHUB2011 - ANR-11-INBS-0010 - INBS - VALID, Infrastructures - Centre français de phénomique végétale - - PHENOME2011 - ANR-11-INBS-0012 - INBS - VALID, Biotech - Bioressources - Développer de nouvelles variétés de blé pour une agriculture durable - - BREEDWHEAT2010 - ANR-10-BTBR-0003 - BTBR - VALID, University of Sheffield [Sheffield], Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Plateforme Bordeaux Metabolome, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Bordeaux, MetaboHUB-MetaboHUB, FSOV {2016K], Metabolomics Society, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), ANR-11-INBS-0012,PHENOME,Centre français de phénomique végétale(2011), ANR-10-BTBR-0003,BREEDWHEAT,Développer de nouvelles variétés de blé pour une agriculture durable(2010), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Plateforme Métabolome [Bordeaux] (PMB), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

Session : Plant, food, enviromenal and microbial, Poster P-422; International audience

Published
2019

29. Front. plant sci

Author

ROCH, Léa, Dai, Zhanwu, GOMES, Eric, Bernillon, Stéphane, Wang, Jiaojiao, Gibon, Yves, Moing, Annick, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Organic Acids, Cross-Species, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Inter-Species, Metabolism Regulation, Qualité du fruit, Amino Acids, Primary Metabolism, Plante Fruitière, Sugars, Fleshy Fruit, Développement du fruit
Abstract

UMR BFP - Equipe Métabolisme; Although fleshy fruit species are economically important worldwide and crucial for human nutrition, the regulation of their fruit metabolism remains to be described finely. Fruit species differ in the origin of the tissue constituting the flesh, duration

Published
2019
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31. Combined metabolomic and proteomic profiling of maize leaf to reveal metabolic responses to cold temperatures

Author

Urrutia, Maria, Blein-Nicolas, Melisande, Bernillon, Stéphane, Deborde, Catherine, Maucourt, Mickaël, Jacob, Daniel, Ballias, Patricia, Benard, Camille, Sellier, Hélène, Gibon, Yves, Giauffret, Catherine, Zivy, Michel, Moing, Annick, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), AgroParisTech-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Métabolome [Bordeaux] (PMB), Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité Expérimentale Grandes Cultures Innovation Environnement - Picardie (GCIE), Agroressources et Impacts environnementaux (AgroImpact), Plateforme d'Analyse Protéomique de Paris Sud Ouest (PAPPSO), and Réseau Francophone de Métabolomique et Fluxomique

Subjects
proteomics, Métabolisme, environmental changes, [SDV]Life Sciences [q-bio], maize leaf, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, metabolomics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

32. Annotation of oak leaf metabolome for genetic and ecological studies

Author

Bernillon, Stéphane, Leprovost, Grégoire, Lalanne, Céline, Moing, Annick, Brachi, Benjamin, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Réseau Francophone de Métabolomique et Fluxomique, and Gauthier, Muriel

Subjects
[SDV] Life Sciences [q-bio], Métabolome, Chêne, Métabolisme, [SDV]Life Sciences [q-bio], Métabolite, Feuille, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Etude génétique, Etude écologique, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

33. Primary metabolism investigation of fleshy fruit species using 1H-NMR profiling

Author

Roch, Léa, Cakpo, Coffi Belmys, Deborde, Catherine, Maucourt, Mickael, Berton, Thierry, Bernillon, Stéphane, Arrivault, Stéphanie, Andrieu, Marie-Hélène, Beauvoit, Bertrand, Colombie, Sophie, CLAVE, Anaïs, BALLIAS, Patricia, Benard, Camille, Vercambre, Gilles, Génard, Michel, Dai, Zhanwu, Gibon, Yves, Moing, Annick, ProdInra, Migration, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB), Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, and Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

34. Mycotoxin biosynthesis and central metabolism are two interlinked pathways in Fusarium graminearum, as demonstrated by the extensive metabolic changes induced by caffeic acid exposure

Author

Chéreau, Sylvain, Atanasova-Penichon, Vessela, Legoahec, Laurie, Bernillon, Stéphane, Deborde, Catherine, Maucourt, Mickael, Verdal-Bonnin, Marie-Noëlle, Pinson-Gadais, Laetitia, Moing, Annick, Ponts, Nadia, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and Universität für Bodenkultur Wien [Vienne, Autriche] (BOKU). AUT.

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

35. MeRy-B: a web knowledgebase for the storage, visualization, analysis and annotation of plant NMR metabolomic profiles

Author

Bernillon Stéphane, Moing Annick, Deborde Catherine, Gil Laurent, Ferry-Dumazet Hélène, Rolin Dominique, Nikolski Macha, de Daruvar Antoine, and Jacob Daniel

Subjects
Botany, QK1-989
Abstract

Abstract Background Improvements in the techniques for metabolomics analyses and growing interest in metabolomic approaches are resulting in the generation of increasing numbers of metabolomic profiles. Platforms are required for profile management, as a function of experimental design, and for metabolite identification, to facilitate the mining of the corresponding data. Various databases have been created, including organism-specific knowledgebases and analytical technique-specific spectral databases. However, there is currently no platform meeting the requirements for both profile management and metabolite identification for nuclear magnetic resonance (NMR) experiments. Description MeRy-B, the first platform for plant 1H-NMR metabolomic profiles, is designed (i) to provide a knowledgebase of curated plant profiles and metabolites obtained by NMR, together with the corresponding experimental and analytical metadata, (ii) for queries and visualization of the data, (iii) to discriminate between profiles with spectrum visualization tools and statistical analysis, (iv) to facilitate compound identification. It contains lists of plant metabolites and unknown compounds, with information about experimental conditions, the factors studied and metabolite concentrations for several plant species, compiled from more than one thousand annotated NMR profiles for various organs or tissues. Conclusion MeRy-B manages all the data generated by NMR-based plant metabolomics experiments, from description of the biological source to identification of the metabolites and determinations of their concentrations. It is the first database allowing the display and overlay of NMR metabolomic profiles selected through queries on data or metadata. MeRy-B is available from http://www.cbib.u-bordeaux2.fr/MERYB/index.php.

Published
2011
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36. Maize metabolome and proteome responses to controlled cold stress partly mimic early‐sowing effects in the field and differ from those of Arabidopsis.

Author

Urrutia, Maria, Blein‐Nicolas, Mélisande, Prigent, Sylvain, Bernillon, Stéphane, Deborde, Catherine, Balliau, Thierry, Maucourt, Mickaël, Jacob, Daniel, Ballias, Patricia, Bénard, Camille, Sellier, Hélène, Gibon, Yves, Giauffret, Catherine, Zivy, Michel, and Moing, Annick

Subjects
INDUCTIVE effect, CORN, CORN breeding, GLUTATHIONE transferase, METABOLISM, ARABIDOPSIS
Abstract

In Northern Europe, sowing maize one‐month earlier than current agricultural practices may lead to moderate chilling damage. However, studies of the metabolic responses to low, non‐freezing, temperatures remain scarce. Here, genetically‐diverse maize hybrids (Zea mays, dent inbred lines crossed with a flint inbred line) were cultivated in a growth chamber at optimal temperature and then three decreasing temperatures for 2 days each, as well as in the field. Leaf metabolomic and proteomic profiles were determined. In the growth chamber, 50% of metabolites and 18% of proteins changed between 20 and 16°C. These maize responses, partly differing from those of Arabidopsis to short‐term chilling, were mapped on genome‐wide metabolic maps. Several metabolites and proteins showed similar variation for all temperature decreases: seven MS‐based metabolite signatures and two proteins involved in photosynthesis decreased continuously. Several increasing metabolites or proteins in the growth‐chamber chilling conditions showed similar trends in the early‐sowing field experiment, including trans‐aconitate, three hydroxycinnamate derivatives, a benzoxazinoid, a sucrose synthase, lethal leaf‐spot 1 protein, an allene oxide synthase, several glutathione transferases and peroxidases. Hybrid groups based on field biomass were used to search for the metabolite or protein responses differentiating them in growth‐chamber conditions, which could be of interest for breeding. Metabolomic and proteomic responses of diverse maize hybrids to progressive decreasing temperatures were larger from 20 to 16°C, partly mimicked those in the field, and involved primary and specialized metabolisms. [ABSTRACT FROM AUTHOR]

Published
2021
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37. Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

Author

Huang, Huichuan, Nguyen Thi Thu, Thuy, He, Xiahong, Gravot, Antoine, Bernillon, Stéphane, Ballini, Elsa, Morel, Jean-Benoit, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Faculty of Agronomy, Hue University of Agriculture and Forestry (HUAF), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Natural Science Foundation of Yunnan Province of China : 2016FD025, Vietnamese government, Chinese government, ANR-SYSTERRA program, MetaboHUB program : ANR-11-INBS-0010, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, and Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)

Subjects
Vegetal Biology, rice, food and beverages, Plant Science, Magnaporthe oryzae, fertilizer, nitrogen, defense, glutamine, notrogen, pathogenicity, effector, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, protection des cultures, Biologie végétale, Original Research
Abstract

BFP Equipe MétabolismeBGPI : équipe 4; International audience; Modifications in glutamine synthetase OsGS1-2 expression and fungal pathogenicity underlie nitrogen-induced susceptibility to rice blast.Understanding why nitrogen fertilization increase the impact of many plant diseases is of major importance. The interaction between Magnaporthe oryzae and rice was used as a model for analyzing the molecular mechanisms underlying Nitrogen-Induced Susceptibility (NIS). We show that our experimental system in which nitrogen supply strongly affects rice blast susceptibility only slightly affects plant growth. In order to get insights into the mechanisms of NIS, we conducted a dual RNA-seq experiment on rice infected tissues under two nitrogen fertilization regimes. On the one hand, we show that enhanced susceptibility was visible despite an over-induction of defense gene expression by infection under high nitrogen regime. On the other hand, the fungus expressed to high levels effectors and pathogenicity-related genes in plants under high nitrogen regime. We propose that in plants supplied with elevated nitrogen fertilization, the observed enhanced induction of plant defense is over-passed by an increase in the expression of the fungal pathogenicity program, thus leading to enhanced susceptibility. Moreover, some rice genes implicated in nitrogen recycling were highly induced during NIS. We further demonstrate that the OsGS1-2 glutamine synthetase gene enhances plant resistance to M. oryzae and abolishes NIS and pinpoint glutamine as a potential key nutrient during NIS.

Published
2017
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38. Tomato Guanylate-Binding Protein SlGBP1 Enables Fruit Tissue Differentiation by Maintaining Endopolyploid Cells in a Non-Proliferative State.

Author

Musseau, Constance, Jorly, Joana, Gadin, Stéphanie, Sørensen, Iben, Deborde, Catherine, Bernillon, Stéphane, Mauxion, Jean-Philippe, Atienza, Isabelle, Moing, Annick, Lemaire-Chamley, Martine, Rose, Jocelyn K.C., Chevalier, Christian, Rothan, Christophe, Fernandez-Lochu, Lucie, and Gévaudant, Frédéric

Published
2020
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39. The timing of shifts in the transcriptome and proteome in relation to metabolic state during tomato fruit development

Author

Belouah, Isma, Balliau, Thierry, Benard, Camille, Beauvoit, Bertrand, Colombie, Sophie, Bernillon, Stéphane, Ballias, Patricia, Cabasson, Cecile, Maucourt, Mickael, Deborde, Catherine, Moing, Annick, Biais, Benoit, Rolin, Dominique, Zivy, Michel, Gibon, Yves, Hooks, Mark, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), and Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA)

Subjects
physiologie végétale, tomate, solanum lycopersicum, proteome, metabolite, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, fruit, tomato, développement des fruits, métabolisme, transcriptome, métabolomique
Abstract

The timing of shifts in the transcriptome and proteome in relation to metabolic state during tomato fruit development. Metabolomics 2016. 12. Annual Conference of the Metabolomics Society

Published
2016

40. Plant Metabolomics at Bordeaux Metabolome Facility, a member of MetaboHUB and PHENOME IA projects. Tools and Applications

Author

Deborde, Catherine, Ballias, Patricia, Bénard, Camille, Bernillon, Stéphane, Berton, Thierry, Cabasson, Cécile, Cocureau, Virginie, Diarrassouba, Salimata, Gibon, Yves, Jacob, Daniel, Lefebvre, Marie, Maucourt, Mickael, Rolin, Dominique, Roques, Simon, Fouillen, Laetitia, Mongrand, Sebastien, Bessoule, Jean-Jacques, Domergue, Frederic, Le Guedard, Marina, Testet, Eric, Moing, Annick, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), ADERA, Université de Bordeaux (UB), Metabolomics Society., and ProdInra, Migration

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

41. Metabolomic study of tolerance to water limitation for sunflower germination

Author

Berton, Thierry, Bernillon, Stéphane, Maucourt, Mickael, Deborde, Catherine, Falagan Sama, Natalia, Saux, Marine, Bouteau, Hayat, Bailly, Christophe, Moing, Annick, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Université Pierre et Marie Curie (Paris 6), ProdInra, Archive Ouverte, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), and Université Pierre et Marie Curie - Paris 6 (UPMC)

Subjects
graine de tournesol, stress abiotique, abiotic stress, sunflower, helianthus annuus, water, sunflower seed, sunflowers, tournesol, water stress, physiologie végétale, eau, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, stress hydrique, germination de graine, métabolisme, métabolomique
Abstract

Metabolomic study of tolerance to water limitation for sunflower germination. Metabolomics 2016. 12. Annual Conference of the Metabolomics Society

Published
2016

42. Integrative and predictive biology outside the plant: linking computational tools to fleshy fruit systems

Author

Cabasson, Cecile, Colombie, Sophie, Beauvoit, Bertrand, Nazaret, Christine, Benard, Camille, Dieuaide Noubhani, Martine, Rolin, Dominique, Bernillon, Stéphane, Deborde, Catherine, Jacob, Daniel, Moing, Annick, Maucourt, Mickael, Belouah, Isma, Roch, Léa, Dartigues, Benjamin, Nikolski, Macha, Mazat, Jean-Pierre, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
Metadata, Fruit development, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Primary metabolism, Metabolic flux modelling, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

43. A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana

Author

BONNOT, Clémence, PINSON, Benoît, CLÉMENT, Mathilde, BERNILLON, Stéphane, CHIARENZA, Serge, KANNO, Satomi, KOBAYASHI, Natsuko I., DELANNOY, Etienne, NAKANISHI, Tomoko M, NUSSAUME, Laurent, DESNOS, Thierry, Plant Environmental Physiology and Stress Signaling (PEPSS), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Tokyo University of Science [Tokyo], Graduate School of Agricultural and Life Sciences [UTokyo] (GSALS), The University of Tokyo (UTokyo), ANR-09-BLAN-0118,CHEMIGENA,Génétique d'une voie limitant la croissance racinaire d'Arabidopsis en réponse à la carence phosphatée(2009), ANR-08-KBBE-0006,FOSSI,Phosphate signalling(2008), Signalisation de l'Adaptation des Végétaux à l'Environnement (SAVE), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Graduate School of Agricultural and Life Sciences, Department of Biomaterial Sciences, and The University of Tokyo

Subjects
phosphate starvation, Full Paper, Arabidopsis thaliana, Arabidopsis Proteins, Research, [SDV]Life Sciences [q-bio], Arabidopsis, Oryza sativa, Isoxazoles, Full Papers, Plants, Genetically Modified, Plant Roots, Phosphates, Small Molecule Libraries, chemical genetics, phosphate homeostasis, Gene Expression Regulation, Plant, PHOSTIN, Ubiquitin-Conjugating Enzymes, Homeostasis, phr1, phl1 mutant, ComputingMilieux_MISCELLANEOUS, Signal Transduction, Transcription Factors
Abstract

International audience; Plants display numerous strategies to cope with phosphate (Pi)-deficiency. Despite multiple genetic studies, the molecular mechanisms of low-Pi-signalling remain unknown. To validate the interest of chemical genetics to investigate this pathway we discovered and analysed the effects of PHOSTIN (PSN), a drug mimicking Pi-starvation in Arabidopsis. We assessed the effects of PSN and structural analogues on the induction of Pi-deficiency responses in mutants and wild-type and followed their accumulation in plants organs by high pressure liquid chromotography (HPLC) or mass-spectrophotometry. We show that PSN is cleaved in the growth medium, releasing its active motif (PSN11), which accumulates in plants roots. Despite the overaccumulation of Pi in the roots of treated plants, PSN11 elicits both local and systemic Pi-starvation effects. Nevertheless, albeit that the transcriptional activation of low-Pi genes by PSN11 is lost in the phr1;phl1 double mutant, neither PHO1 nor PHO2 are required for PSN11 effects. The range of local and systemic responses to Pi-starvation elicited, and their dependence on the PHR1/PHL1 function suggests that PSN11 affects an important and early step of Pi-starvation signalling. Its independence from PHO1 and PHO2 suggest the existence of unknown pathway(s), showing the usefulness of PSN and chemical genetics to bring new elements to this field.

Published
2016
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44. Leaf marker metabolites for low nitrogen stress in maize

Author

Urrutia Rosauro, Maria, Bernillon, Stéphane, Maucourt, M., Deborde, Catherine, Jacob, Daniel, Ballias, Patricia, Sellier, H., Gibon, Yves, Quillere, Isabelle, Hirel, Bertrand, Giauffret, Catherine, Moing, Annick, ProdInra, Archive Ouverte, Unité d'Agronomie de Laon-Reims-Mons (AGRO-LRM), Institut National de la Recherche Agronomique (INRA), Inconnu, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Agroressources et Impacts environnementaux (AgroImpact), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Leaf marker metabolites, nitrogen stress, maize
Abstract

CT2 ; Département BAP; Leaf marker metabolites for low nitrogen stress in maize. Plant Biology Europe EPSO/FESPB 2016

Published
2016

45. Flux balance modelling in developing tomato fruit: the respiration climacteric as an emergent property

Author

Colombie, Sophie, Beauvoit, Bertrand, Nazaret, Christine, Bénard, Camille, Cabasson, Cécile, Maucourt, Mickael, Bernillon, Stéphane, Moing, Annick, Dieuaide Noubhani, Martine, Mazat, Jean-Pierre, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB), Centre National de la Recherche Scientifique (CNRS), Réseau Francophone de Métabolomique et Fluxomique (RFMF). FRA., and ProdInra, Migration

Subjects
Based model, Constraint, Flux balance analysis, Respiration climateric, Central metabolism, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Carbon allocation, Environmental stress, Metabolic flux modelling, Tomato fruit development, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

46. Combining LCMS data from two years for plant performance biomarkers discovery

Author

Urrutia Rosauro, Maria, Bernillon, Stéphane, LAMARI, Nadia, Maucourt, Mickael, Ballias, Patricia, SELLIER, Hélène, Gibon, Yves, Giauffret, Catherine, Moing, Annick, ProdInra, Migration, Agroressources et Impacts environnementaux (AgroImpact), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité Expérimentale Grandes Cultures Innovation Environnement - Picardie (GCIE), and Metabolomics Society.

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

47. Using metabolomic approaches and modelling of central metabolic to study fleshy fruit quality

Author

Colombie, Sophie, Beauvoit, Bertrand, Rolin, Dominique, Bernillon, Stéphane, Bénard, Camille, Prodhomme, Duyên, Maucourt, Mickael, Andrieu, Marie-Hélène, Ballias, Patricia, Deborde, Catherine, Cabasson, Cécile, Moing, Annick, Gallusci, Philippe, Teyssier, Emeline, Stammitti, Linda, Bertrand, Anne, Hooks, Mark, Dieuaide Noubhani, Martine, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

48. Application of metabolomics to the biochemical phenotyping of resistant maize lines to Fusarium graminearum

Author

Gauthier, Léa, Atanasova-Penichon, Vessela, Bernillon, Stéphane, Chéreau, Sylvain, Maucourt, Mickael, Moing, Annick, Mey, G., Surault, A., Roussel, M., Chopin, F., Fouquet, R., Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Euralis Semences, Monsanto Company, Réseau Francophone de Métabolomique et Fluxomique (RFMF). FRA., and ProdInra, Migration

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract

National audience

Published
2015

49. Assessment of metabolome compartmentation changes during tomato leaf development using nonaqueousfractionation

Author

DE JAHAM, Clémence, Thiombiano, Benjamin, Benard, Camille, Alonso, A. P., Cocuron, J. C., Larbat, Romain, Cabasson, Cecile, Vercambre, Gilles, Bernillon, Stéphane, Colombie, Sophie, Beauvoit, Bertrand, Andrieu, Marie-Hélène, Gibon, Yves, Dieuaide Noubhani, Martine, ProdInra, Archive Ouverte, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Ohio State University [Columbus] (OSU), Laboratoire Agronomie et Environnement (LAE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Biologie du fruit et pathologie ( BFP ), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique ( INRA ) -Université Sciences et Technologies - Bordeaux 1, Ohio State University [Columbus] ( OSU ), Laboratoire Agronomie et Environnement ( LAE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lorraine ( UL ), Unité de recherche Plantes et Systèmes de Culture Horticoles ( PSH ), Institut National de la Recherche Agronomique ( INRA ), and Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)

Subjects
développement des feuilles, [ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology, tomate, physiologie végétale, métabolome, solanum lycopersicum, fleshy fruit, metabolite, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, fruit charnu, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, tomato, métabolisme
Abstract

UMR BFP - Equipe Métabolisme; Assessment of metabolome compartmentation changes during tomato leaf development using nonaqueousfractionation. 12. Solanaceae Conference SOL 2015

Published
2015

50. Cold treatment affects the metabolite profiles of maize leaves

Author

LAMARI, Nadia, Zhendre, Vanessa, Bernillon, Stéphane, Maucourt, Mickael, Deborde, Catherine, Jacob, Daniel, Rolin, Dominique, SELLIER, Hélène, Gibon, Yves, Giauffret, Catherine, Moing, Annick, ProdInra, Migration, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité Expérimentale Grandes Cultures Innovation Environnement - Picardie (GCIE), Institut National de la Recherche Agronomique (INRA), Agroressources et Impacts environnementaux (AgroImpact), and Réseau Francophone de Métabolomique et Fluxomique (RFMF). FRA.

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

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