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OBJECTIVES/GOALS: The aim of this study is to analyze electronic health record (EHR) data using Mapper PLUS (MP), a new mathematical model, to classify acute myocardial infarction (MI) patients by risk of major adverse events (AE). We tested MP’s ability to define patient subgroups with distinctive risk for death, heart failure or recurrent MI after revascularization. METHODS/STUDY POPULATION: An EHR retrospective analysis of 797 MI patients and 29 variables (i.e., laboratory tests, imaging, vitals, and clinical traits) collected at the time of hospitalization was conducted. All patients received percutaneous coronary intervention and standard pharmacotherapy. MP analysis produced a multi-dimensional nodal graph of the patients based on similarities found within variables. Two algorithms, Walk Likelihood and Walk Likelihood Community Finder were applied to the graph which formed joint clusters according to spatial distance within nodes. The final output was three clusters for risk level evaluation. Risk level (low vs. high) was relative to the average risk of AEs for the entire cohort one year post MI. RESULTS/ANTICIPATED RESULTS: Of three patient subgroups, one (n= 318) had a >1 fold change for the probability of survival without AE when compared to the overall cohort and thus was defined as the low-risk group. The second group (n=304) had DISCUSSION/SIGNIFICANCE: MP stratifies patients into three groups according to predictive variables which relate to the risk for AE following an acute MI treatment. This is a new topological method for patient classification based on minimal input strictly from pre-collected EHR data. More cohort studies are needed to validate MP to classify patients for precision medicine.

Abstract Objectives The urgent need for novel antibiotics cannot be overemphasized. Hafnia alvei A23BA was isolated from plant rhizosphere as part of an effort to recover novel antibiotic-producing bacterial strains from soil samples. The genome of the isolate was sequenced to facilitate mining for potential antibiotic-encoding biosynthetic gene clusters and to gain insights into how these gene clusters could be activated. Data description Here, we report the complete genome sequence of H. alvei A23BA obtained from the hybrid assembly of Illumina HiSeq and GridION reads. The genome, consisting of a circular chromosome and a circular plasmid, is 4.77 Mb in size with a GC content of 48.77%. The assembly is 99.5% complete with genomic features including 4,217 CDSs, 125 RNAs, and 30 pseudogenes. Thiopeptide, beta-lactone, siderophore, and homoserine lactone biosynthetic gene clusters were also identified. Other gene clusters of interest include those associated with bioremediation, biocontrol, and plant growth promotion- all of which are reported for H. alvei for the first time. This dataset serves to expedite the exploration of the biosynthetic and metabolic potentials of the species. Furthermore, being the first published genome sequence of a soil isolate, this dataset enriches the comparative genomics study of H. alvei strains.

Background. Chronic exposure to arsenic is a major health concern consequent upon generation of excessive reactive oxygen species. The safety of treatment with chelating agents has not been well established; therefore, there is a need for a paradigm shift in the approach to management of arsenic toxicity. Bioflavonoids are known to influence redox homeostasis in cells; the study therefore investigates the efficacy of quercetin and its zinc and iron metal complexes on sodium arsenite (NaAr)-intoxication in rats. Methods. Spectroscopic study of quercetin hydrate and its metal complexes was performed using UV-Vis and FT-IR spectrometer. Furthermore, twenty male Wistar rats were obtained and equally divided into four groups, treated orally and daily for 28 days with 10 mg/kg NaAr, 30 mg/kg quercetin, quercetin-zinc, and quercetin-iron separately. Five more rats were used as control. Plasmatic aspartate transferase (AST), alanine transferase (ALT), creatinine (CREA), and total protein (TP) were estimated. Levels of kidney and liver lipid peroxidation (LPO), glutathione (GSH), catalase (CAT), and glutathione-S-transferase (GST) were determined. Histology was used to view the lesions. Results. Treatment of arsenic-toxicity with quercetin and its complexes decreased the activities of ALT, AST, CREA, TP, CAT, and GST and concentration of LPO and GSH. Quercetin-zn treatment showed a better result than quercetin-iron in the liver. Histology results showed absence of lesions in quercetin zinc and iron treatment in both the kidney and the liver. Conclusion. Quercetin zinc and iron increased the bioavailability of quercetin and therefore could be relevant as adjuvants in arsenic poisoning.

Background. Chronic exposure to arsenic is a major health concern consequent upon generation of excessive reactive oxygen species. The safety of treatment with chelating agents has not been well established; therefore, there is a need for a paradigm shift in the approach to management of arsenic toxicity. Bioflavonoids are known to influence redox homeostasis in cells; the study therefore investigates the efficacy of quercetin and its zinc and iron metal complexes on sodium arsenite (NaAr)-intoxication in rats. Methods. Spectroscopic study of quercetin hydrate and its metal complexes was performed using UV-Vis and FT-IR spectrometer. Furthermore, twenty male Wistar rats were obtained and equally divided into four groups, treated orally and daily for 28 days with 10 mg/kg NaAr, 30 mg/kg quercetin, quercetin-zinc, and quercetin-iron separately. Five more rats were used as control. Plasmatic aspartate transferase (AST), alanine transferase (ALT), creatinine (CREA), and total protein (TP) were estimated. Levels of kidney and liver lipid peroxidation (LPO), glutathione (GSH), catalase (CAT), and glutathione-S-transferase (GST) were determined. Histology was used to view the lesions. Results. Treatment of arsenic-toxicity with quercetin and its complexes decreased the activities of ALT, AST, CREA, TP, CAT, and GST and concentration of LPO and GSH. Quercetin-zn treatment showed a better result than quercetin-iron in the liver. Histology results showed absence of lesions in quercetin zinc and iron treatment in both the kidney and the liver. Conclusion. Quercetin zinc and iron increased the bioavailability of quercetin and therefore could be relevant as adjuvants in arsenic poisoning. [ABSTRACT FROM AUTHOR]

Most variants of bacteria are resistant to traditional antibiotics which are organic. To overcome the growing infections, bacteria resistant infections, and multiple drug resistance (MDR) rates, transition metals with biological importance were coordinated to organic ligands (Ibuprofen and Salicylic acid) with anti-inflammatory properties. In this study, metal complexes of mixed Ibuprofen and Salicylic acid were prepared using a standard method to give of the type [M(Ibu)(Sal)X] (where M = Fe2+, Ni2+, Cu2+, and X = Cl2, Ibu = Ibuprofen, Sal = Salicylic acid). The complexes were characterized by UV-visible spectroscopy, conductivity measurements, melting points, FT-IR, and X-ray diffraction. The metal ions are coordinated to the ligands via the carboxylato oxygen donor atoms of both ligands. From the physicochemical data, the complexes are non-electrolytes. The XRD study suggested that the metal complexes possess a well-defined crystalline structure with average crystallite sizes of < 62 nm. Evaluations of the antimicrobial activities of the ligands and their complexes against gram-positive bacteria (S. aureus, B. subtilis, S. faecalis) and gram-negative bacteria (K. pneumonia, E. coli, and P. aeroginosa) via standard method were utilized to determine the zones of inhibition. The complexes exhibited a higher zone of inhibition, indicating higher antimicrobial activities when compared to the parent ligand. The results revealed that the metal-drug complexes are promising chemotherapeutic agents with wide spectrum of activities. [ABSTRACT FROM AUTHOR]

Context: Professional society guidelines are evidence-based recommendations intended to promote standardized care and improve health outcomes. In the context of increased recognition of the role racism has played in shaping medical practices, many healthcare researchers and practitioners have critiqued existing guidelines, particularly those that include race-based recommendations. These critiques question how implicit--and sometimes explicit--racism has influenced both the evidence that guidelines are based on and the interpretation of that evidence. Objective and study design: In this study, we conducted a scoping review of all professional guidelines focused on the treatment and management of essential hypertension published between 1977 and 2020 and examined how race and ethnicity influenced guideline recommendations. Results: We found that authors largely focused on Black and White categories, with few references to other groups. Guideline authors described race as a distinct, preestablished category with biological underpinnings (for example, recommended specific medication classes to a predefined racial group). No guideline discussed the history of racism and the role it has played in perpetuating hypertension (or other health) disparities or inequities. The characterizations of race in essential hypertension guidelines normalize race as a biological rather than social construct. Conclusion: As the health system grapples with how to address racism in medicine, exploring how professional care guidelines reflect an outdated understanding of race can help us understand both the social origins of and treatments for hypertension inequities. [ABSTRACT FROM AUTHOR]