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Your search keyword '"Amarnath Mukherjee"' showing total 3 results
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3 results on '"Amarnath Mukherjee"'

1. PROL1 is essential for xenograft tumor development in mice injected with the human prostate cancer cell-line, LNCaP, and modulates cell migration and invasion

Author

Amarnath Mukherjee, Augene Park, and Kelvin Paul Davies

Subjects
cell motility, cell invasiveness, opiorphin, prol1, prostate cancer, Medicine (General), R5-920
Abstract

Background and objective: A growing body of literature suggests modulated expression of members of the opiorphin family of genes (PROL1, SMR3A and SMR3B) is associated with cancer. Recently, overexpression of PROL1 was shown to be associated with prostate cancer, with evidence of a role in overcoming the hypoxic barrier that develops as tumors grow. The primary goal of the present studies was to support and expand evidence for a role of PROL1 in the development and progression of prostate cancer. Material and methods: We engineered knock-out of the opiorphin gene, PROL1, in LNCaP, an androgen-sensitive, human prostate cancer derived, cell-line. Using xenograft assays, we compared the ability of injected LNCaP PROL1 knock-out cell-lines to develop tumors in both castrated and intact male mice with the parental LNCaP and LNCaP PROL1 overexpressing cell-lines. We used RNAseq to compare global gene expression between the parental and LNCaP PROL1 knock-out cell-lines. Wound closure and 3D spheroid invasion assays were used to compare cell motility and migration between parental LNCaP cells and LNCaP cells overexpressing of PROL1. Results: The present studies demonstrate that LNCaP cell-lines with consisitutive knock-out of PROL1 fail to develop tumors when injected into both castrated and intact male mice. Using RNAseq to compare global gene expression between the parental and LNCaP PROL1 knock-out cell-lines, we confirmed a role for PROL1 in regulating molecular pathways associated with angiogenesis and tumor blood supply, and also identified a potential role in pathways related to cell motility and migration. Through the use of wound closure and 3D spheroid invasion assays, we confirmed that overexpression of PROL1 in LNCaP cells leads to greater cell motility and migration compared to parental cells, suggesting that PROL1 overexpression results in a more invasive phenotype. Conclusion: Overall, our studies add to the growing body of evidence that opiorphin-encoding genes play a role in cancer development and progression. PROL1 is essential for establishment and growth of tumors in mice injected with LNCaP cells, and we provide evidence that PROL1 has a possible role in progression towards a more invasive, metastatic and castration resistant prostate cancer (PrCa).

Published
2022
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2. MICROWAVE DETECTION OF INAUDIBLE NOISE FROM HIGH-VOLTAGE TRANSMISSION LINES

Author

Monika Soni and Amarnath Mukherjee

Subjects
RF, GIS, JTCL, Inaudible Noise, PLC
Abstract

Landscape features, that affect the route of the line are the most significant part of this research. These features are incorporated into raster-based GIS, and using information gathered from stakeholders, the route with minimum opposition is developed between two substations. Routing with acceptable measured parameters of a line is also affected by the rules and regulations in a particular state. In this work, a brief introduction is given to the main legal requirements involved in this regard. Non-point impacts that are independent of terrain are also given in detail. Potential stakeholders, and their importance are also explained in this work. To carry out the proposed work, we will measure the radio frequency by not using the instrument used to measure the inaudible noise in the conventional way. With this, we will not acquire cost separately and the available resources will also be used, because different types of RF instruments are better than microphones for measuring radio frequency.

Published
2021
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3. Real-time, Near-Infrared Fluorescence Imaging with an Optimized Dye/Light Source/Camera Combination for Surgical Guidance of Prostate Cancer

Author

Brian Neuman, Rong Ma, Amarnath Mukherjee, Ying Chen, Ronnie C. Mease, Ronald Rodriguez, John B. Eifler, Martin G. Pomper, Wasim H. Chowdhury, Mark Castanares, and Shawn E. Lupold

Subjects
Diagnostic Imaging, Male, Cancer Research, Pathology, medicine.medical_specialty, Near-Infrared Fluorescence Imaging, Fluorophore, Spectrophotometry, Infrared, Swine, medicine.medical_treatment, Article, chemistry.chemical_compound, Prostate cancer, Mice, In vivo, medicine, Medical imaging, Animals, Fluorescent Dyes, Prostatectomy, business.industry, Cancer, Prostatic Neoplasms, Prostate-Specific Antigen, medicine.disease, Prostate-specific antigen, Disease Models, Animal, Oncology, chemistry, Heterografts, Laparoscopy, business, Biomedical engineering
Abstract

Purpose: The prostate-specific membrane antigen (PSMA) is a surface glycoprotein overexpressed on malignant prostate cells, as well as in the neovasculature of many tumors. Recent efforts to target PSMA for imaging prostate cancer rely on suitably functionalized low-molecular-weight agents. YC-27 is a low-molecular-weight, urea-based agent that enables near-infrared (NIR) imaging of PSMA in vivo. Experimental Design: We have developed and validated a laparoscopic imaging system (including an optimized light source, LumiNIR) that is capable of imaging small tumor burdens with minimal background fluorescence in real-time laparoscopic extirpative surgery of small prostate tumor xenografts in murine and porcine models. Results: In a mouse model, we demonstrate the feasibility of using real-time NIR laparoscopic imaging to detect and surgically remove PSMA-positive xenografts. We then validate the use of our laparoscopic real-time NIR imaging system in a large animal model. Our novel light source, which is optimized for YC-27, is capable of detecting as little as 12.4 pg/mL of the compound (2.48-pg YC-27 in 200-μL agarose). Finally, in a mouse xenograft model, we demonstrate that the use of real-time NIR imaging can reduce positive surgical margins (PSM). Conclusions: These data indicate that a NIR-emitting fluorophore targeted to PSMA may allow improved surgical treatment of human prostate cancer, reduce the rate of PSMs, and alleviate the need for adjuvant radiotherapy postoperatively. Clin Cancer Res; 21(4); 771–80. ©2014 AACR.

Published
2014

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