1. Molecular Analysis of Plasmodium ovale Variants
- Author
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Thin Thida Win, Amadu Jalloh, Indah Setyawati Tantular, Takafumi Tsuboi, Marcelo Urbano Ferreira, Masatsugu Kimura, and Fumihiko Kawamoto
- Subjects
Plasmodium ovale ,variant isolate ,classic isolate ,molecular analysis ,SSUrRNA gene ,cysteine protease gene ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Complete DNA sequences of the small subunit ribosomal RNA (SSUrRNA) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type Plasmodium ovale isolates from Southeast Asia and compared with GenBank-available data. Three different SSUrRNA sequences (Pov 1–3) were found in each variant-type isolate, and two different SSUrRNA sequences (Poc 1–2) in each classic-type isolate. Pov 1–3 were closer to sequences previously found in the Cameroon and MAL/MAI isolates, whereas Poc 1–2 were closer to sequences previously found in two clones of the Nigerian I/CDC strain. The 3′ half of Pov 1–3 was identical to the partial sequence of the SSUrRNA gene from the London School (LS) strain. Results support grouping P. ovale into two groups, the classic type (including the Nigerian I/CDC strain) and the variant type (Cameroon, MAL/MAI, and LS isolates).
- Published
- 2004
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