1. Sense and antisense modification of glial alpha B-crystallin production results in alterations of stress fiber formation and thermoresistance
- Author
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Toru Iwaki, Jun Tateishi, Akiko Iwaki, and James E. Goldman
- Subjects
DNA, Complementary ,Hot Temperature ,Alpha (ethology) ,Cell morphology ,Transfection ,DNA, Antisense ,Cell Line ,Transformation, Genetic ,Hsp27 ,Heat shock protein ,Sense (molecular biology) ,Cell Adhesion ,Tumor Cells, Cultured ,Animals ,Humans ,Cytoskeleton ,Heat-Shock Proteins ,Rous sarcoma virus ,biology ,Cell Biology ,Articles ,biology.organism_classification ,Molecular biology ,Crystallins ,eye diseases ,Rats ,Gene Expression Regulation, Neoplastic ,Cell culture ,biology.protein ,sense organs ,Neuroglia - Abstract
The phenotypic effects of selectively altering the levels of alpha B-crystallin in cultured glial cells were analyzed using sense and antisense approaches. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat alpha B-crystallin sense cDNA or an antisense cDNA regulated by a Rous sarcoma virus promoter to alter cellular levels of alpha B-crystallin. The antisense strategy resulted in decreased alpha B-crystallin levels, as revealed by Western blot and immunocytochemical analyses. The reduced alpha B-crystallin expression was accompanied by alterations in cellular phenotype: (a) a reduction of cell size and/or a slender cell morphology; (b) a disorganized microfilament network; and (c) a reduction of cell adhesiveness. Like HSP27, the presence of additional alpha B-crystallin protein confers a thermoresistant phenotype to stable transfectants. Thus, alpha B-crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization. more...
- Published
- 1994