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Purpose: Time and Space assembly line balancing problem (TSALBP) is the problem of balancing the line taking the area required by the task and to store the tools into consideration. This area is important to be considered to minimize unplanned traveling distance by the workers and consequently unplanned time waste. Although TSALBP is a realistic problem that express the real-life situation, and it became more practical to consider multi-manned assembly line to get better space utilization, few literatures addressed the problem of time and space in simple assembly line and only one in multi-manned assembly line. In this paper the problem of balancing bi-objective time and space multi-manned assembly line is proposed Design/methodology/approach: Hybrid genetic algorithm under time and space constraints besides assembly line conventional constraints is used to model this problem. The initial population is generated based on conventional assembly line heuristic added to random generations. The objective of this model is to minimize number of workers and number of stations. Findings: The results showed the effectiveness of the proposed model in solving multi-manned time and space assembly line problem. The proposed method gets better results in solving real-life Nissan problem compared to the literature. It is also found that there is a relationship between the variability of task time, maximum task time and cycle time on the solution of the problem. In some problem features it is more appropriate to solve the problem as simple assembly line than multi-manned assembly line. Originality/value: It is the first article to solve the problem of balancing multi-manned assembly line under time and area constraint using genetic algorithm. A relationship between the problem features and the solution is found according to it, the solution method (one sided or multi-manned) is defined.

Grass pea is a promising crop with the potential to provide food and fodder, but its genomics has not been adequately explored. Identifying genes for desirable traits, such as drought tolerance and disease resistance, is critical for improving the plant. Grass pea currently lacks known R-genes, including the nucleotide-binding site-leucine-rich repeat (NBS-LRR) gene family, which plays a key role in protecting the plant from biotic and abiotic stresses. In our study, we used the recently published grass pea genome and available transcriptomic data to identify 274 NBS-LRR genes. The evolutionary relationships between the classified genes on the reported plants and LsNBS revealed that 124 genes have TNL domains, while 150 genes have CNL domains. All genes contained exons, ranging from 1 to 7. Ten conserved motifs with lengths ranging from 16 to 30 amino acids were identified. We found TIR-domain-containing genes in 132 LsNBSs, with 63 TIR-1 and 69 TIR-2, and RX-CCLike in 84 LsNBSs. We also identified several popular motifs, including P-loop, Uup, kinase-GTPase, ABC, ChvD, CDC6, Rnase_H, Smc, CDC48, and SpoVK. According to the gene enrichment analysis, the identified genes undergo several biological processes such as plant defense, innate immunity, hydrolase activity, and DNA binding. In the upstream regions, 103 transcription factors were identified that govern the transcription of nearby genes affecting the plant excretion of salicylic acid, methyl jasmonate, ethylene, and abscisic acid. According to RNA-Seq expression analysis, 85% of the encoded genes have high expression levels. Nine LsNBS genes were selected for qPCR under salt stress conditions. The majority of the genes showed upregulation at 50 and 200 μM NaCl. However, LsNBS-D18, LsNBS-D204, and LsNBS-D180 showed reduced or drastic downregulation compared to their respective expression levels, providing further insights into the potential functions of LsNBSs under salt stress conditions. They provide valuable insights into the potential functions of LsNBSs under salt stress conditions. Our findings also shed light on the evolution and classification of NBS-LRR genes in legumes, highlighting the potential of grass pea. Further research could focus on the functional analysis of these genes, and their potential use in breeding programs to improve the salinity, drought, and disease resistance of this important crop.