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Kaplan, Suleyman/0000-0003-1477-5002 WOS: 000387093200007 OBJECTIVE: To investigate the effects of pentylenetetra-zole (PTZ) kindling on the number of pyramidal neurons in the rat hippocampus. STUDY DESIGN: Using unbiased stereology, we calculated total pyramidal neuron numbers in the CA1, CA2, and CA3 areas and the whole hippocampus after PTZ-induced kindling. The estimation and the analysis of the number of hippocampal pyramidal cells (areas of CA1-3) were done with the optical fractionator technique, which is a stereological counting technique. RESULTS: Stereological analysis showed that the total number of pyramidal neurons in the whole hippocampus and the number of pyramidal neurons in CA1 were significantly lower in kindled rats as compared to the controls (p0.05). CONCLUSION: PTZ-induced seizures cause a decrease in the amount of pyramidal neurons in the CA area of the rat hippocampus.

Karakurt, Meryem Dilek Acar/0000-0002-2314-8126; Sefil, Fatih/0000-0001-5534-7448; BOSTANCI, Mehmet Omer/0000-0002-2887-1073 WOS: 000361557500028 PubMed: 26379841 Gap junctions play an important role in the synchronized neuronal discharges. The main reason of the epileptic seizures is disruption of this synchronization. Therefore, the aim of the present study is to explore the combination valproic acid with carbenoxolone in pentylenetetrazole-kindled rats. In the first set of experiments, pentylenetetrazole (35 mg/kg intraperitoneally was administered to the rats to produce the kindling and then permanent screw electrodes to record electroencephalographic signals. The kindled rats were divided into six groups. While electroencephalographic recordings received from animals, behavioral evaluation was done by an observer. The data analysis was performed using T test and Mann-Whitney U tests. The dose of 40 mg/kg carbenoxolone was the most effective in carbenoxolone treatment groups. It prevented generalized seizures by 50%, reduced seizure stage, seizure duration and spike frequency. There was no significant difference between carbenoxolone-valproic acid combination and valproic acid on any seizure parameters. The current study is the first study which shows the interaction of carbenoxolone with valproic acid in pentylenetetrazole kindling model. As a result, carbenoxolone-valproic acid combination was not more effective than the standalone use of these drugs. Ondokuz Mayis University Research FoundationOndokuz Mayis University [PYO.TIP.1904.09.010] This project was supported by the Ondokuz Mayis University Research Foundation (PYO.TIP.1904.09.010).