1. Microsatellite instability status affects gene expression profiles in early onset colorectal cancer patients
- Author
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Gulcin Tezcan, Abdullah Zorluoglu, Gulsah Cecener, Unal Egeli, Berrin Tunca, Ersin Öztürk, Secil Ak, Tuncay Yilmazlar, Omer Yerci, Elif Erturk, Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Bölümü., Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Bölümü., Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Bölümü., Ak, Seçil, Tunca, Berrin Türkei, Yılmazlar, Tuncay, Tezcan, Gülçin, Çeçener, Gülşah, Egeli, Ünal, Öztürk, Ersin, Yerci, Ömer, Ertürk, Elif, ABI-6078-2020, AAH-3843-2020, AAH-1420-2021, F-8554-2017, AAP-9988-2020, and AAK-3371-2021
- Subjects
Male ,Secondary ,Genetic testing ,Heredity ,Colorectal cancer ,Carcinogenesis ,Gene ,CK20 gene ,Metastasis ,Gene expression regulation ,DNA methylation ,REG1A ,Prognosis ,Gene expression profiling ,MAP3K8 ,Proto-oncogene proteins ,Human ,Clinical article ,Oncoprotein ,Inferior Mesenteric Artery ,Complete ,Lymph Node Dissection ,REG1A gene ,Carcinomas ,Article ,MAP3K8 gene ,MAP3K8 protein, human ,Upregulation ,Genetic screening ,Genetics ,Humans ,BAT26 gene ,Colorectal tumor ,neoplasms ,Cancer recurrence ,Alpha ,Cancer prognosis ,Lymph node metastasis ,Microsatellite instability ,Dna ,D5S346 gene ,Follow up ,medicine.disease ,digestive system diseases ,Onset age ,Gene expression regulation, neoplastic ,D2S123 gene ,Reg-ivcytokeratin-20 ,Genetic markers ,Surgery ,Microsatellite stability ,Identification ,Survival ,Overexpression ,Keratin-20 ,Turkey (republic) ,Lithostathine ,REG1A protein, human ,Middle aged ,CK20 ,Priority journal ,Differentiation ,Population genetic parameters ,Distant metastasis ,Colorectal carcinogenesis ,Female ,Adult ,Turkish population ,Messenger-rna expression ,Adolescent ,Age of onset ,Lymphatic metastasis ,Biology ,Colorectal neoplasms ,BAT25 gene ,medicine ,Chemotherapy ,Mitogen activated protein kinase kinase kinase ,Human tissue ,Mortality ,Cytokeratin 20 ,Genetic marker ,MSI ,MSS ,Microsatellite marker ,Keratin 20 ,MAP kinase kinase kinases ,Biological marker ,Young adult ,Cancer research ,Cancer patient ,D17S250 gene ,Transcriptome ,Controlled study ,Early onset - Abstract
Background: The association between microsatellite instability (MSI) status and gene expression profiles in the early onset sporadic colorectal cancer (CRC) has not been clearly established. The aim of this study was to identify the altered gene expression patterns depending on the MSI status of early onset CRC and determine specific biomarkers that could provide novel therapeutic molecular targets in the Turkish population. Materials and methods: MSI markers (BAT25, BAT26, D2S123, D5S346, and D17S250) were investigated in tumors from 36 early onset sporadic CRC patients in whom gene expression profiles were analyzed previously. The relationship between the gene expression profiles depending on MSI status was evaluated. Results: A total of 15 tumors (16.66%) were identified as having MSI and 21 tumors (58.33%) were identified as having microsatellite stability (MSS). CK20 and MAP3K8 upregulation, observed in MSS tumors, was significantly associated with lymph node metastasis, recurrence, and/or distant metastasis and a short median survival (P < 0.05). REG1A upregulation is also correlated with recurrence and/or distant metastasis and a short median survival in patients with MSI tumors (P < 0.05). Conclusions: High expression levels of CK20 and MAP3K8 in MSS tumors and REG1A in MSI tumors correlated with a poor prognosis in CRC patients. Further studies and validations are required; these genes may provide novel therapeutic molecular targets for the development of anticancer drugs related to MSI status for early onset CRC treatment.
- Published
- 2013