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Copyright of Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

羟基酪醇是一种标志性多酚，其油溶性差且容易降解，将羟基酪醇修饰为油酸酯是提高其油溶性的有效途径。对羟基酪醇油酸酯的酶法合成进行了研究，考察了脂肪酶种类、酶添加量、醇酸摩尔比、溶剂(2-甲基-2-丁醇)用量、反应时间对合成反应的影响，并利用Box-Behnken中心组合的响应面设计优化了羟基酪醇油酸酯的合成反应条件。另外，采用质谱和核磁分析对产物进行了表征。结果表明：羟基酪醇油酸酯合成的最佳工艺条件为羟基酪醇500 mg、油酸2.474 g(醇酸摩尔比1∶ 2.7)、脂肪酶Novozym 435添加量2.2%(以整个反应体系的质量为基准)、溶剂用量2.6 mL、反应温度37 ℃和反应时间7 h，在此条件下羟基酪醇转化率高达89.6%；质谱和核磁分析证明成功合成了羟基酪醇油酸酯。脂肪酶催化合成羟基酪醇油酸酯的技术突破，可为延伸油橄榄产业链及副产物高值利用提供有力支撑。 Hydroxytyrosol is a major polyphenol, it has poor oil solubility and is prone to degrade. Modifying hydroxytyrosol into hydroxytyrosyl oleate is an effective way to improve hydroxytyrosol′s oil solubility. The enzymatic synthesis of hydroxytyrosyl oleate was constructed, and the effects of lipase type, enzyme dosage, molar ratio of hydroxytyrosol to oleic acid, solvent （2-methyl-2-butanol）dosage and reaction time on the synthesis of hydroxytyrosyl oleate were investigated. Then, the enzymatic synthesis conditions of hydroxytyrosyl oleate was optimized by response surface design of Box-Behnken central combination. In addition, the product was characterized by mass spectrometry and nuclear magnetic analysis. The results showed that the optimal synthesis conditions of hydroxytyrosyl oleate were obtained as follows: hydroxytyrosol dosage 500 mg, oleic acid dosage 2.474 g (molar ratio of hydroxytyrosol to oleic acid 1∶ 2.7), lipase Novozym 435 dosage 2.2% (based on the reaction system mass), solvent dosage 2.6 mL, reaction temperature 37 ℃ and reaction time 7 h. Under the optimal conditions, the conversion rate of hydroxytyrosol was the highest, reaching 89.6%. Mass spectrometry and nuclear magnetic analysis showed that the hydroxytyrosyl oleate was successfully synthesized. The technological breakthrough in the lipase-catalyzed synthesis of hydroxytyrosyl oleate can not only strengthen the extension of the olive industry chain, but also promote the high-value utilization of the by-products in the olive industry.

为了拓展生物柴油原料来源，以气生微藻Heveochlorella sp. Yu为研究对象，通过测定其生长曲线、生物量、油脂产率、沉降率等，对其作为生物柴油生产原料的特性进行研究。结果表明，培养后气生微藻Heveochlorella sp. Yu的生物量为4.14 g/L，油脂含量为39.43%，油脂产率为181.38 mg/(L·d)，2 h的自然沉降率为65.28%（可大幅浓缩水体，降低微藻的采收成本）。此外，该微藻能够产生γ-氨基丁酸（GABA），含量为9.50 mg/g。气生微藻Heveochlorella sp. Yu具有成为生物柴油原料的潜力，具有微藻油脂的开发价值。 In order to expand the source of biodiesel feedstock, the characteristics of aerial microalgae Heveochlorella sp. Yu as raw material for biodiesel were studied by measuring its growth curve, biomass, lipid productivity, sedimentation rate, etc. The results showed that the microalgae biomass after cultivation was 4.14 g/L, the lipid content was 39.43%, and the lipid productivity was 181.38 mg/(L·d). The natural sedimentation rate of aerial microalgae Heveochlorella sp. Yu within 2 h was 65.28% (which could greatly concentrate the water body and reduce the harvesting cost of microalgae). In addition, aerial microalgae Heveochlorella sp. Yu could produce gamma-aminobutyric acid (GABA) with a content of 9.50 mg/g. The aerial microalgae Heveochlorella sp. Yu had the potential to be used as a feedstock for biodiesel and had the development value of microalgae lipid.