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The aim of this work was the activation of biopolymer material based on wild flax cake (Camelina Sativa) with eucalyptus and rosemary essential oils added in different concentrations (0.5%, 1% and 2%). The wild flax cake, left over after the cold pressing of the oil, was used to obtain biopolymer films. To obtain active packaging, essential oils were added to this biopolymer material and the influence of the addition of essential oils on the physico-chemical, mechanical, barrier and biological properties of biopolymer films based on wild flax cake was examined. The obtained results showed the most significant influence on water vapor permeability. The control sample had a water vapor permeability value of 5.43 g/m 2 h, while in the sample with 2% added eucalyptus essential oil, this value was 3.14 g/m 2 h. Antioxidant activity was also confirmed in the control sample without added essential oils (60.10%), but with the addition of 2% eucalyptus essential oil, this value increased to 78.54%. Eucalyptus essential oil proved to be more effective than rosemary oil on the investigated properties of the films. The addition of essential oils to biopolymer films broadens the spectrum of functional properties, including the improvement of mechanical, barrier and biological properties.

In this paper, zein-based films with the addition of shellac were synthesized by lamination on the existing dry zein film (L samples) and by adding shellac alcohol solution during the synthesis of zein film in a ratio of 50-50 by casting process (M samples). Zein films without the addition of shellac were designated as control samples. Physico-chemical (thickness, moisture content and solubility), mechanical (tensile strength and elongation at break) and barrier characteristics (water vapor permeability) were examined for all samples. The resulting films were transparent, glossy, light yellow (control) to ocher (samples with shellac), flexible, and non-sticky. The results showed significantly higher values of elongation at the break of the samples with shellac (11.49% for M and 18.99% for L) compared to the control (7.14%). Significantly lower water vapor permeability values were found, 10.04 g/(m²ꞏh) for L, and 18.41 g/(m²ꞏ h) for M, compared to the control pure zein film (40.33 g/(m² ꞏh)).

This study aimed at investigating the synthesis of biopolymer films based on the Camelina sativa cake, an agricultural waste which remains after cold pressing of the oil from seed. During the film synthesis, three different granulations of the camelina cake were used: the whole cake, fractions with a size of 180-250 µm and fractions smaller than 180 µm. Half of the samples were pre-treated with an ultrasonic bath in order to examine the influence of the native mucilage from the cake on the properties of the obtained films. The biopolymer film samples were tested for mechanical, barrier, physicochemical and structural properties. The obtained films were dark, firm and flexible. Application of mucilage removal pre-treatment contributed to lower tensile strength and higher elongation at break. Significantly lower water vapour permeability was recorded in the samples not pre-treated regarding mucilage removal. A foil with optimal physicochemical characteristics was produced using oilcake with a particle size less than 180 µm, regardless of the pre-treatment application. There were no structural differences or differences in thermal behaviour among the tested samples. Statistical analysis (Z-Score analysis) showed the sample CSoC˂180,wo was optimal due to good mechanical, barrier and physicochemical properties.

The main aim of this research was to apply biopolymer coatings on osmotically dehydrated mushrooms and monitor their quality during storage. Mushrooms were osmotically dehydrated in sugar beet molasses (80% dry matter) under optimized conditions (45 °C for 5 hours), as previously reported elsewhere. Two different biopolymers were chosen: chitosan, a polysaccharide polymer, and zein, a protein polymer. A non-treated mushroom sample was chosen as a control sample. The mushroom samples were analysed for sugar and protein content, as well as water loss and microbiological profile. An increase in sugar content was the most noticeable in the osmotically dehydrated mushrooms compared to the control sample due to the use of molasses as a hypertonic solution. The contribution of used biopolymer coatings to the sugar and protein content of the coated and osmotically treated mushrooms was negligible. Chitosan coating contributed to better storage stability of treated mushrooms by lowering the moisture loss and microbial count. For this reason, chitosan treated sample was chosen for further examination related to the evaluation of its baking potential as a filling in a traditional stuffed pie-like layered bakery product - burek. Burek was stuffed with fresh mushrooms, osmotically treated mushrooms or osmotically treated mushrooms coated with chitosan. The sensorial assessment proved that control burek and burek samples with osmotically dehydrated mushrooms coated with chitosan were the most preferred groups based on odour and overall impression.

Among many important requirements for packaging materials, environmental friendliness is a property that has become necessary for any material that needs to be competitive in the market. Life Cycle Analysis (LCA) is an analytical instrument that provides a framework for analyzing the impact of products and services on the environment, i.e. provides an understanding and the possibility of comparing different products. LCA studies the use of resources and the consequent emissions of pollutants on the environment during the entire life of a product from raw materials exploitation, through production, use and treatment at the end of the life cycle - recycling and final disposal. This paper will provide an overview of the LCA results of various commercial polymer packaging materials, as well as the results of LCAs of biopolymer materials. Although LCA faces the problem of data heterogeneity, since some studies focused on individual segments of the analysis, while some related to all aspects of the process, as well with the problem of interpreting heterogeneous results, because the output parameters were arbitrarily selected by the researcher, still it could be concluded that the available LCA studies and environmental assessments support further development of biobased polymers. When comparing biopolymer materials with commercial synthetic polymers, they have advantages - lower consumption of fossil fuels and lower emission rate of greenhouse gases from the whole life cycle.

There are many biodegradable and recyclable packaging materials available, alternatives for plastics: paper and cardboard; biodegradable polyethene (degradable due to additives incorporated during production, whose role is to lead to the polyethylene breakdown into CO2, H2O, biomass and minerals when in landfill) and biodegradable plastic (made from renewable biomass-biopolymers in a relatively energy-efficient process). The decomposition routes of degradable materials are reflected in the degradation for which realization a physico-chemical stimulus is required and biodegradation for which microorganisms are responsible. The global biodegradable plastic market was valued at $1.6 billion in 2019 and it is expected to reach $4.2 billion by 2027. The largest segment by application of biodegradable materials is in packaging with a market share of more than 60%. Some examples of degradable packaging existing on the market will be presented in the paper.

Lipid oxidation is a major cause of off-flavors and the loss of nutrients in fat-containing foods and oils. The prevention or retardation of those deteriorative reactions of oil during processing and storage is required. Commercially available synthetic packaging materials are causing serious environmental problems due to their non-degradability. To reduce environmental impacts associated with synthetic plastics, biopolymer materials have a great potential to be used instead. Further, biopolymers play an important role in food preservation because of their antioxidant and antimicrobial activities. The packing system is comprised of natural active materials that can improve the shelf life of oil-packed, minimize oxidation and improve mechanical, barrier and biological properties of biopolymer films. This paper aims to review currently available literature in the field of biopolymer materials application for packaging different types of edible oils.

This paper examines the effect of starch coating and the osmotic dehydration in sugar beet molasses on the microbiological stability of apples. One-half of the osmotically treated/untreated apples were protected by starch coating, resulting in four sample groups (namely the K, P, OD and OD+P sample groups). E. coli, Salmonella spp. and Listeria monocytogenes were not detected in any of the samples. Enterobacteria were present in the K and P samples in the first four days (indicating a downward trend), but were not subsequently detectable. The total number of microorganisms (TVC) was found to be uniform in each sample group. However, the TVC values were significantly higher in the K and P sample groups than those of the OD and OD + P samples. Yeasts and molds were detected in the K and P samples, whereas the presence of yeasts and molds in the OD and OD+P samples was confirmed only after four days of storage. The results obtained indicate that osmotic dehydration is a suitable method for maintaining microbial stability, whereas starch coating did not justify its purpose.

Mushrooms (Agaricus bisphorus) were osmotically dehydrated in sugar beet molasses solutions at concentrations of 60%, 70% and 80%d.m., at operating temperatures of 25 °C, 35 °C and 45 °C during 0.5h, 1h, 1.5h, 2h, 3h, and 5 hours. Moisture content, water activity (aw), microbiological parameters (total plate counts, Enterobacteriaceae, total yeasts and moulds count) and content of mineral elements (potassium, magnesium, iron and calcium) were determined in the osmodehydrated mushroom samples. Response surface methodology and analysis of variance were selected to estimate the main effects of the process variables (temperature, time, concentration) on process performance and selected mushroom attributes (microbiological counts, chemical composition and mineral content). Increase in the values of applied osmotic process parameters led to the significant increase in the content of minerals (for example, an increase in K and Ca content by 269.42% and 939.03%, respectively) and a decrease in aw values (from 0.941 to 0.811), decrease in microbiological load and relative protein content (decrease by 33.07%), indicating a possibility for prolonged shelf life and suitability for further processing. The osmodehydrated mushrooms could be considered as ingredients for new functional (semi)products due to improved nutritive profile.

Biopolymer-based materials present good alternatives for synthetic materials. However, their high water sensitivity may limit their usage for food products packaging. Addition of hydrophobic components into the material formulation could improve this property. In this work 3, 4 and 5 % (v/v) of Satureja montana or Ocimum basilicum essential oil was incorporated into biopolymer films based on pumpkin oil cake. The obtained materials were analyzed regarding mechanical, physicochemical, barrier and structural properties. Incorporation of the essential oils increased the thickness of the pumpkin oil cake film. Significant reductions in moisture sensitivity, related to physicochemical properties and water vapor transmission rate (almost for 30 %), were observed (p < 0.05). Improvement of light barrier properties was also observed so that the visible light transmission was decreased for around 50 % while the UV light transmission was lower than 1 %. The obtained FTIR spectra confirmed the presence of added essential oils in pumpkin oil cake films, as well as their influence on the reduction in the film surface hydrophilicity. However, mechanical properties, tensile strength and elongation at break, decreased significantly (p < 0.05). These results suggest that incorporation of Satureja montana or Ocimum basilicum essential oil improved barrier properties of pumpkin oil cake-based films and reduced the film affinity toward water.

In this work, pumpkin seed oil cake that remains after process of cold pressed oil extraction, was used for the production of composite biopolymer based packaging films. During the production of films, four degrees of filtration were applied. The obtained composite biopolymer films were analyzed for antioxidant activity using three different antioxidant activity assays: the ability to capture free radicals, including •DPPH radical and hydroxyl radical, •OH, as well as reduction power assay of the analyzed films. By comparing the antioxidant activity of the films obtained with different degrees of filtration, it can be concluded that the films obtained by finer filtration (smaller diameter of the filter pore) exhibit greater ability to capture •DPPH radicals, as well as higher reducing power, while the ability to capture hydroxyl radicals showed different trend. Films obtained from the coarser filtered fractions (higher diameter of the filtrate medium) showed greater •OH scavenging ability.

Biopolymer films can be used as packaging materials in the food industry, when they contribute to a better mechanical integrity of packaged foods and act as barrier to various environmental agents. The greatest disadvantage regarding biopolymer films application are poor barrier characteristic, which is a consequence of their high hydrophilicity; their mechanical and physico-chemical properties are considered to be a moderate disadvantage. This paper presents the effects of chemical modifications, such as the addition of hydrophobic components (essential oils), to the properties of biopolymer films. Except that the addition of essential oils significantly affects the mechanical, barrier and physico-chemical properties of biopolymer films, in this way, biopolymer films become active and exhibit antimicrobial and antioxidant action. Changes in the mechanical and barrier properties of various biopolymer films have been observed in particular, with and without the addition of oil in different concentrations.

Active films based on pumpkin oil cake, with 1 % and 2 % of winter savory and basil essential oil, were synthesized in the paper, and the antioxidant activity of the films obtained was subsequently examined. A biopolymer film based on pumpkin oil cake without the addition of essential oils was used as a control. The following methods were used for determining the antioxidant activity of the films: the DPPH radical scavenging activity, the hydroxyl radical scavenging activity and the reducing power assay. The control film showed some antioxidant activity (DPPH radical scavenging activity = 61 %, hydroxyl radical scavenging activity = 33 %, reducing power = 0.534). However, the addition of essential oils increased the antioxidant activity of the films, especially their reducing power, where as the film with 2 % of winter savory essential oil exhibited the highest activity. A slight increase in the DPPH radical and the hydroxyl radical scavenging activities was also observed with the incorporation of essential oils into the experimental films.

Packaging is inseparable retainer of almost all food products. However, most of produced packaging ends as packaging waste after consumption of the product. Increasing amounts of packaging waste that should be managed represents serious challenge of every modern society. There are many different approaches to address this subject, amongst which biodegradable, natural biopolymer-based or even edible packaging holds considerable potential. In this paper, a by-product of edible oil industry, left after completed extraction by cold pressing of oil from hulls of pumpkin seeds, was used to produce biopolymer packaging films. Pumpkin oil cake was used to produce composite bio-based films. Different filtration of film forming suspension was applied in order to test composite film production using different filtration fractions, leading to higher process yield and minimizing waste. In addition, films were casted on surface, about ten times larger comparing to the cast surface typically reported for these types of films in the literature, in order to test the possibility for commercial production and scale up process. Also, casted mass of film forming suspension was varied in order to define minimal casting mass per unit area. Presented results showed that biopolymer films based on the pumpkin oil cake can be successfully produced in sheets (50x35 cm), compared to films earlier produced in the form of discs with diameter 12 cm. Different filtration fractions from initial film forming suspension can be used for film formation, leading to increased production yield. Different filtration fractions lead to different film properties that should be adjusted according to selected application. Casted mass of film forming suspension was successfully decreased (comparing to earlier literature data) without compromising film functional properties and minimal casting mass was defined as 26 g/m2.

In this paper, the application of a biopolymer coating based on chitosan was tested. The optimized coating was formed as a two-layer coating, wherein the first layer (which is in contact with the product) was formed of chitosan with emulsified caraway essential oil as an active component, whereas the second layer was formed of chitosan with the addition of beeswax to optimize the moisture barrier of the coating. As a substrate, the traditional dry fermented sausage Petrovská klobása was selected. The coating was applied to the sausage after drying. During two months of storage under controlled conditions, the influence of the coating on the loss of moisture, the oxidative stability and the sensory characteristic of aroma of the control sausage (without the coating) and sausage with the coating was monitored. The results obtained indicate a significant contribution of the applied dual layer chitosan-based coating to the preservation of the sausage quality parameters analyzed.

The purpose of this paper is the synthesis and characterization of biodegradable composite films based on whole sunflower oil cake, as well as the effect of process parameters (pH and temperature)on the film properties obtained. Films were synthesized at different temperatures (room temperature, 60 °C and 90 °C) and pH values (10 and 12). The film samples obtained were characterized by the mechanical, barrier and physicochemical properties determination. The experimental results showed that the tensile strength values increased with temperature and pH value increments, whereas the sample with the highest elongation at break value was 60 °C/pH12. The water vapour permeability values were uniform (8.62 g/m2h - 11.63 g/m2h), and lower values were recorded in samples synthesized at higher temperatures. The results obtained relative to light transmission were uniform, and the maximum transmission value was reached at 800 nm (24.1- 35.7 %). The values obtained for swelling and solubility parameters are characteristic of the nature of biopolymer films.

Fatty acid composition of Ricotta cheese filled bakery product was examined using a GC-MS method immediately after production and packaging in the case of a control sample, and after production, packaging under air atmosphere in a seven-layer packaging material consisting of PE/Ad/PA/Ad/PE/Ad/PET, and storing during a four weeks period at room temperature, in the case of the experimental samples. The statistical significance of the fatty acid profile change was examined using ANOVA method. The results of this research showed that there are no significant changes of fatty acids composition and content after defined storing period, with the exception of diunsaturated cis,cis-9,12-octadecadienoic (linoleic) acid, whose average content was reduced by 83.705%. However, a small amount of linoleic acid was converted to cis,trans-9,11-octadecadienoic (conjugated linoleic) acid. Therefore, it could be considered as appropriate to pack and storage Ricotta-filled pastry for the period of four weeks, considering the insignificant changes of fatty acid composition and content.

In this study, three biopolymer films were synthesized: zein (protein), gelatin (protein), and starch (polysaccharide) films and characterized by determining mechanical and physico-chemical properties. Gelatin films proved to be the strongest (tensile strength 84 N/15 mm), while the zein were most flexible (elongation at break 41.6 %). Moisture content was higher in starch films, which is a result of the hydrophilic nature of the polysaccharide films. Swelling degree was 22.5 % for zein, 90.9 % for gelatin, while the highest average value was recorded in starch film samples (840.6 %). Starch films had highest value of solubility degree (36.5 %), while zein film had 27.9 % and gelatin film had 11.85 %.Obtained characterization results are consequences of the different structure of the raw materials and synthesis routes. All undesirable characteristics could be improved by optimizing the composition of the film, as well as synthesis of composite/laminated films.

In this paper, influence of increasing concentrations of beeswax on the antimicrobial activity of chitosan-caraway essential oil biofilm was investigated. Selected microorganisms for the test were Salmonella Typhimurium and Listeria monocytogenes. Reduction of S. Typhimurium and L. monocythogenes viable counts in bacterial suspension in the presence of chitosan films with or without added beeswax amounted over 80 % after 24 h contact time, and over 95 % after 3 h of contact time, respectively and influence of added beeswax on growth reduction was not detected. However, when number of S. Typhimurium viable cells was analyzed (log CFU/ml), it was shown that addition of beeswax led to increased activity of films, especially for the films with 36 kg/m3 and 54 kg/m3of beeswax. When films were transferred, after 24 h in bacterial suspension, onto Petri dishes with nutrient agar and incubated for 24 h on 37°C, results also showed beeswax contribution to growth inhibition of S. Typhimurium.

The purpose of this paper is to improve the properties of biopolymer protein monofilms and produce a novel bilayer biopolymer film on the basis of the monofilms analyzed. Biopolymer monolayer films, based on pumpkin oil cake (PuOC) and zein, and a bilayer film, based on PuOC and zein (PuOC/Zein), were produced as a result of the study. The visual, mechanical, physicochemical and structural properties of the films were evaluated. The results obtained showed that the PuOC film exhibited the highest elongation at break, followed by the bilayer film, whereas the zein film showed the lowest elongation at break. However, the zein film showed the highest tensile strength, followed by the PuOC film. The tensile strength of the bilayer PuOC/Zein film was almost 3 times lower than that of the PuOC film, and almost 4 times lower than that of the zein film. The physicochemical properties recorded indicate that the hydrophobic zein film is the least sensitive to moisture, affecting the moisture sensitivity of PuOC film by reducing the moisture content, swelling and total soluble mater of the bilayer PuOC/Zein film. Based on the FTIR spectra, it could be concluded that there are no significant differences between the two sides of the bilayer PuOC/Zein film. Both sides of the bilayer film examined indicated characteristic peaks for protein biopolymer films in the FTIR spectrum.

Bilayer biodegradable films based on pumpkin oil cake (PuOC) and zein, as well as pouches made from these materials, were prepared, and the changes of their mechanical, physicochemical and barrier properties were analyzed during four weeks of storage. Heat seal quality of formed pouches and composition of the gas atmosphere in the pouches were also monitored. The results showed that the bilayer film had a thickness of 300 ± 10 (μm), and no its changes were observed during the storage time. The tensile strength of the tested film increased slightly in the third week, but the elongation at break showed a decreasing trend during the whole storage period. The decreases in the moisture content, total soluble matter and swelling of the obtained film, were also observed. After one month of storage, the O2 transmission rate of tested films, increased from 27 to 64 (ml/m2 24h 1 bar), and the CO2 gas transmission rate from 147 to 188 (ml/m2 24h 1 bar). The heat seal strength of the PuOC/Zein pouches decreased during the whole storage period. The percentage of O2 in PuOC/Zein pouches increased up to 7 times during the storage period; however, the percentage of CO2 decreased up to 18 times already after one week, and then remained stable in the rest of the storage period. These results are, to a smaller extent, due to the gas transmission rate through the material, especially for CO2 , and to a greater extent, due to the low heat seal strength, which decreased through the storage period, and probably influenced the content of the gases in the pouches.[Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 46010]

Osmotic treatment of celery root was studied in two osmotic solutions (sugar beet molasses and a ternary solution of water, sucrose and salt), at three temperatures (20, 35 and 50°C), and three different immersion periods (1, 3 and 5 h), at atmospheric pressure. The aim was to examine the influence of the type of the used hypertonic agent, the temperature and the immersion time on the water loss, solid gain, water activity, dry matter content, antioxidant activity (expressed by DPPH) and colour attributes (described by CIELAB coordinates L*, a* and b*). During the experiments, the antioxidant activity of celery root was increased in sugar beet molasses, while the DPPH value tended to decrease in the ternary solution. The experimental data of osmotic dehydration was used for PCA modelling. The standard scores analysis revealed that the optimum process parameters were gained with an immersion time of 5 h and a temperature of 35°C. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR-31055]

The objective of this study was to examine influence of glycerol and guar-xanthan in different concentrations on bi-layer film based on pumpkin oil cake (PuOC) and corn zein, and to find optimal concentrations of these additives which result in film with optimal mechanical and barrier characteristics. Different concentrations of glycerol (30%, 40%, and 50%) as plasticizer and guar-xanthan (0.1%, 0.3%, and 0.5%) as a stabilizer were added in PuOC layer, while zein layer stayed unchanged in all film compositions. Obtained results showed that the optimal mechanical properties had film with the lowest concentration of glycerol (30%) and the highest concentration of guar-xanthan (0.5%). Films showed poor barrier for water vapour, typical for biopolymer-based films. However, all tested films were very good barrier to O2 (transmission rate lower than 50 ml/m2/day at 1 bar). On the other hand, with increase of concentration of added substances, transmission rate of CO2 increased.

Growing consumer demand for the food without chemical preservatives focused significant extent of research in the direction of finding natural compounds that can be used in food preservation. In this context, natural substances with strong antimicrobial and antioxidant properties, like essential oils, as well as natural biopolymers, particularly draw attention. Natural biopolymers can serve as carriers of the active components, such as essential oils in order of their sustained release to the food during storage, and may themselves exhibit activity in protecting foods from oxidation and/or microbial spoilage. Chitosan has been extensively studied as semi-natural polymer with expressed bioactive properties. While antimicrobial activity of chitosan solution in different acids has been confirmed towards different bacteria, yeasts and moulds, reports concerning intensity, underlying machanism and different factors afecting antioxidant activity of chitosan vary through the available literature. This paper presents a review in the field of antioxidative activity of chitosan with different properties, as well as chitosan based biopolymer films in order to clarify this aspect of chitosan bioactivity and confront different reports found in the literature.

This study investigated properties of starch-based edible films with oregano and black cumin essential oil addition. Essential oils addition positively affected film swelling (decreased due to essential oil addition), mechanical properties (tensile strength decreased while elongation at break increased), and water vapor barrier properties (decreased along with essential oils addition). Control film did not have any biological activity, which proves the need for essential oils addition in order to obtain active packaging. Oregano oil was more effective in terms of biological activity. Endothermal peak, above 200°C, represents total thermal degradation of edible films. Diffraction pattern of control film showed significant destruction of A-type crystal structure. Addition of essential oils resulted in peak shape change: diffraction peaks became narrower. Principal Component Analysis has been used to assess the effect of essential oils addition on final starch-based edible films characteristics with the aim to reveal directions for the film characteristics improvement, since the next phase will be optimal film application for food packaging.

Static mixer is used for premixing action before the main mixing process, for significant reduction of mixing time and energy consumption. In this article, the novel numerical approach called Discrete Element Method is used for modeling of granular flow in multiple static mixer applications, while the Computational Fluid Dynamic method was chosen for fluid flow modeling. The main aim of this article is to predict the behaviour of granules being gravitationally transported in different mixer configuration and to choose the best configuration of the mixer taking into account the total particle path, the number of mixing elements and the quality of the obtained mixture. The results of the numerical simulations in the static mixers were compared to experimental results, the mixing quality is examined by relative standard deviation criterion. The effects of the mixer type and the number of mixing elements on mixing process were studied using analysis of variance (ANOVA).

The aim of this work was to evaluate the simultaneous effect of glycerol (30%, 40%, 50%) and guar-xanthan gum addition (0.1%, 0.3%, 0.5%) effect on starch based edible film mechanical and barrier properties. According to Response Surface Methodology, the most influential parameter in the second order polynomial models calculation was the percentage of glycerol, while the linear term of guar-xanthan content was important only for second order polynomial model calculation for tensile strength and water vapor permeability. According to Principal Component Analysis, samples grouping along the first component are primarily due to the content of glycerol, which is also confirmed by ANOVA analysis. According Cluster Analysis, two separate clusters are observed on the dendrogram, which includes the right (with the increased value of tensile strength) and the left cluster (with the increased value of elongation break and water vapor permeability). The observed distance the two clusters is considerable (approx. 80). [Projekat Ministarstva nauke Republike Srbije, br. TR-31055: Osmotic dehydration of food - energy and environmental aspects of sustainable production]

Quality parameters including sensory and physical characteristics and proximate and mineral composition in M. psoas major, M. semimembranosus, M. longissimus thoracis et lumborum and M. triceps brachii of Swallow-Belly Mangulica pigs were determined. Type of muscles had no significant effect (P>0.05) on water-holding capacity, protein, total fat, K, P and Ca content. The M. psoas major was the highest in pH24h, water-holding capacity, CIEa* and CIEb* values, moisture, K, P aQuality parameters including sensory and physical characteristics and proximate and mineral composition in M. psoas major, M. semimembranosus, M. longissimus thoracis et lumborum and M. triceps brachii of Swallow-Belly Mangulica pigs were determined. Type of muscles had no significant effect (P>0.05) on water-holding capacity, protein, total fat, K, P and Ca content. The M. psoas major was the highest in pH24h, water-holding capacity, CIEa* and CIEb* values, moisture, K, P and Cu content, and the lowest in visual marbling score and total fat content. The M. semimembranosus was the highest in Mg and Ca content, and the lowest in CIEL* value (darkest muscle) and K content. The lightest colour (CIEL* value), the highest content of protein, total fat and total ash, and the lowest visual colour score, pH24h, CIEa* and CIEb* values, content of moisture, Na, Ca, Zn, Fe and Cu were found in M. longissimus thoracis et lumborum. The highest visual colour and marbling score, the highest content of Na, Zn and Fe, and the lowest water-holding capacity, content of protein, total ash, P and Mg were found in M. triceps brachii.

The aim of this study was to investigate starch and starch based edible films with and without oregano essential oil addition in terms of crystallinity by using X-ray diffractometry. Starch crystallinity is related to amylopectin linear chains which may give different X-ray diffraction patterns, described as A, B and C chains. Diffraction pattern of starch based edible film without essential oil addition showed broad diffraction peak in 15-20° 2θ region, indicating destruction of A-type crystal structure due to experimental conditions of casting procedure used in this work. Diffraction peaks of samples with 0.5 %, 1 % and 2 % oregano essential oil, whose shapes are to some extent retained after oregano oil addition, were centered at 20.8°, 20.1° and 21.6° 2θ respectively, while pattern of sample with 2% oregano essential oil showed additional diffraction peaks at 31.1°, 35.1°, 38.3°, 44.5° and 64.9° 2θ. X-ray diffraction patterns showed polymorphous nature of investigated starch samples with essential oils addition.

The application of biopolymer films and coating to food products represents a new approach to reduce r migration of moisture, oxygen, carbon dioxide, aromas, and oil in or out of food. Also, biopolymer films, as packaging materials, need to enhance appearance of food and to improve mechanical integrity. In order to facilitate handling and application films must be strong, but flexible enough. Addition of plasticizer could modify the flexibility of biopolymer films. It is known that guar-xanthan can improve the flexibility of polysaccharides films. Therefore, the objective of this study was to examine the influence of guar-xanthan on characteristics of pumpkin oil cake (PuOC) films, with emphasis on the flexibility of the film. Two approaches of guar-xanthan addition in the pumpkin oil cake were applied - stirring with laboratory stirrer and using of ultra turrax homogenizer. The results showed that guar-xanthan improved physical and mechanical properties of PuOC films, but only if film-forming solution was homogenized by ultra turrax. The films with guar-xanthan slightly improved mechanical properties - tensile strength and elongation at break, water content, solubility and swelling properties, but were much easier for handling.

The aim of this study was to evaluate bioactivity of chitosan film with incorporated caraway essential oil by measuring antioxidant and antimicrobial activity. A Fourier transform infrared spectroscopy was used to determine the potential interaction of functional groups of chitosan film and incorporated caraway essential oil. New detected peaks and main shifts in the peaks of chitosan spectra are attributed mainly to presence of s-(+)-carvone and limonene, the main components of caraway essential oil. The antioxidant activity of chitosan film was analyzed by DPPH method. Chitosan film without incorporated caraway essential oil showed the lowest scavenging ability (29.95%, after 24 h). The addition of different concentrations of caraway essential oil into chitosan film significantly enhanced antioxidant activity of pure chitosan film, reaching the maximum of 95%. ASTM E 2149 - 01 method was performed to evaluate the antimicrobial activity of chitosan films. The reduction of bacteria cell number in contact with examined films was tested on Gram-positive bacteria Staphylococcus aureus and Listeria monocytogenes and Gram-negative bacteria Escherichia coli and Salmonella Typhimurium. The most sensitive bacteria was Staphylococcus aureus and the most resistant bacteria was Salmonella Typhimurium for all tested films. These results suggested that incorporation of caraway essential oil into chitosan film significantly improved its antioxidant and antimicrobial activity. The film showed a great potential to be used as an active packaging material.

In order to extend product shelf life while preserving the quality scientific attention focused to biopolymers research that are base for edible films and coatings production. Another major advantage of this kind of food packaging is their eco-friendly status because biopolymers do not cause environmental problems as packaging materials derived from non-renewable energy sources do. Objective of this work was to review recently studied edible films and coatings - their sources, properties and possible application. As sources for edible biopolymers were highlighted polysaccharides, proteins and lipids. The most characteristic subgroups from each large group of compounds were selected and described regarding possible physical and mechanical protection; migration, permeation, and barrier functions. The most important biopolymers characteristic is possibility to act as active substance carriers and to provide controlled release. In order to achieve active packaging functions emulsifiers, antioxidants and antimicrobial agents can also be incorporated into film-forming solutions in order to protect food products from oxidation and microbial spoilage, resulting in quality improvement and enhanced safety. The specific application where edible films and coatings have potential to replace some traditional polymer packaging are explained. It can be concluded that edible films and coatings must be chosen for food packaging purpose according to specific applications, the types of food products, and the major mechanisms of quality deterioration.

Effective diffusivities of water, sucrose and minerals in osmotic treatment of pork cubes (M. triceps brachii) were calculated using Response Surface Methodology (RSM), with respect to temperature (20, 35 and 50oC) and concentration of sugar beet molasses, (60, 70 and 80% w/w). The numerical solution of Fick's' law for unsteady-state mass transfer in a perfect cube configuration was used to calculate the effective diffusivities of water, sucrose and minerals (Na, K, Ca and Mg). Zugarramurdi and Lupin's model was used to predict the equilibrium condition, which was shown to be appropriate for water loss and solute uptake during osmotic treatment. Effective diffusivity of water was found to be in the range of 6.95×10-10 - 8.03×10-10 m2s-1, the sucrose effective diffusivity was between 6.39×10-10 and 8.25×10-10 m2s-1, while diffusivities for minerals were in the range 6.34×10-10 - 8.82×10-10 m2s-1, for Na, 6.27×10-10 - 7.43×10-10 m2s-1, for K, 6.44×10-10 - 8.94×10-10 m2s-1, for Ca and 3.47×10-10 - 5.66×10-10 m2s-1, for Mg. [Projekat Ministarstva nauke Republike Srbije, br. TR 31055]

The aim of this work was to study the changes in osmotically treated pork meat during rehydration. Meat samples were osmotically treated in sugar beet molasses solution, at temperature of (23±2)°C for 5 hours. After being osmotically treated, meat samples were rehydrated at constant temperature (20- 40°C) during different times (15-60 min) in distilled water. The effective diffusivity were between 8.35 and 9.11•10-10 (m2•s-1) for moisture, 6.30-6.94 • 10-10 (m2•s-1), for Na, 5.73-7.46 10-10 (m2•s-1), for K, 4.43-6.25 • 10-10 (m2•s-1), for Ca, 5.35-6.25 • 10-10 (m2•s-1), for Mg, 4.67-6.78 10-10 (m2•s-1), for Cu, 4.68-5.33 • 10-10 (m2•s-1), for Fe, 4.21-5.04 • 10-10 (m2•s-1), for Zn and 5.44-7.16 10-10 (m2•s-1), for Mn. Zugarramurdi and Lupin’s model was used to predict the equilibrium condition, which was shown to be appropriate for moisture uptake and solute loss during rehydration. [Projekat Ministarstva nauke Republike Srbije, br. TR-31055: Osmotic dehydration of food - energy and environmental aspects of sustainable production]

The main goal in this work was to investigate the structure of starch based edible films with essential oil addition. Films were obtained from water solution containing starch, polyol, guar-xantan gum modified mixture and essential oil by casting it on a Petri dish. Black cumin essential oil was added in three different concentrations. Starch based edible film without essential oil was used as a control. Physical, mechanical and structural properties are determined: thickness, tensile strength, elongation at break and film structure. FTIR results pointed to quantitative law dependency between amount of essential oil and spectra absorption values. Based on dependency coefficient of correlation was calculated (R2=0.99483).

The main objective of this article was to investigate the use of the diffusive model, Zugarramurdi and Lupin's model, Peleg's model, second order polynomial model and Weibull-type model for the prediction of water loss and solid gain, during osmotic treatment of fish (Carassius gibelio) in sugar beet molasses solution. The accepted experimental design allowed to assess the effect of solution concentration (60-80% w/w), temperature (10, 20, 35 and 50oC) and immersion time (1-5h), and to quantify water and solid mass transfer coefficients. The considered empirical models were able to represent satisfactorily the transfers of water and solids during all osmotic treatments.

In this paper, beeswax and caraway essential oil were added as hydrophobic compounds to chitosan film after synthesizing, to improve its water vapor resistance. Influence of these two hydrophobic compounds on visual properties, structure and water content of chitosan film was investigated. Results showed that addition of 1 % of caraway oil, as well as beeswax affected visual properties of the chitosan film, which became yellowish, opaque and turbid. Addition of caraway essential oil did not alter original structure of chitosan film (FTIR ATR technique), while addition of beeswax affected chitosan film structure. Water content in the films decreased with the addition of beeswax. This is a very important change that could be used in chitosan film functionality improvement for packaging application.

In this work, caraway or oregano essential oils were added to the cast chitosan film in an attempt to design a natural, biodegradable, bioactive (antioxidant and antimicrobial) packaging film. When the structure of the tested films was analyzed with Fourier transform infrared spectroscopy, no significant differences were found between the chitosan film with and without essential oils of caraway or oregano. The lowest antioxidant activity, based on the scavenging of the stable DPPH• free radical, was detected for the chitosan film without oil addition, going from 11.64%, after 2.5 h to 28.96%, after 24 h. When the caraway essential oil was added to the chitosan film, the antioxidant activity was increased (p

Present study investigated effects of plasticizer contribution on structural, barrier and mechanical properties of corn starch based edible films. Films were obtained from solutions (containing gelatinized starch, polyol, guar-xantan gum modified mixture and water) by casting it on a Petri dish. Sorbitol acted as a plasticizer and guar-xantan modified mixture had role to enable better film folding and handling. Sorbitol was used in concentrations: 100, 130 and 160% (dry starch basis). The film proved to be strong, moderately flexible with good barrier properties to oxygen and water vapor. It was estimated that oxygen and water vapor permeability increases as plasticizer content in the film increases. Mechanical strength of films decreases due to plasticizer addition.

After functional phase, packaging becomes waste that is recycled or disposed of in landfills. Recently, numerus packages have been developed for assessing the packaging risk on the environment. We applied Gabi 4 Education software on polymer product packaging for meat products. The objective of first part of the paper was characterization of materials used for meat and meat products packaging in terms of mechanical and barrier properties. Results show that tested materials are able to keep protective atmosphere and contribute to the quality and sustainability of the product. Air permeability was 3.60 and 26.60 ml/m224h, and water vapor was 6.90 and 9.50 ml/m224h, respectively, for foils 1 and 2, as a result of different film composition. In second part, based on real data, Gabi 4 Education software is applied. The obtained results showed that organic compounds emissions have the highest impact on human health and the most damaging environmental impact observed was the emission of CO2.

Chitosan, the second most abundant polysaccharide in nature, after cellulose, has been tested for numerous applications, among which for edible film and coating. Chitosan-based coating showed positive results for shelf life prolongation of meet products. In this paper, dry fermented sausage (Petrovská klobása) was coated with chitosan-caraway film. The effect of coating on the moisture content, color and lipid oxidation was investigated during a fivemonth period of storage. The moisture content decreased rapidly during the storage and the coating did not slow down the loss of moisture. The Lightness (L*) of the sausage surface increased by the coating application, while the redness (a*) and yellowness (b*) did not change. The coated sausages showed a better color stability of the sausage core through the storage time. Also, coated sausage showed a better oxidative stability till the 60th day of storage, while this difference was not detected at the end of the storage period. Apart from slowing down sausage drying during the storage, chitosan-caraway coating was effective in preserving the sausage quality. [Projekat Ministarstva nauke Republike Srbije, br. TR31032]

When deciding what packaging is the most appropriate for a product there are many factors to be considered. One of them is the impact of the packaging on environment. In this work, life cycle inventory and life cycle assessment of two different volume packagings were compared. The data were collected on the types and amounts of materials and energy consumption in the process of packaging and distribution of hand cream packed in polypropylene jars of 200 and 350 mL. Life cycle inventory (LCI) and life cycle impact assessment (LCA) were calculated. It was found that the total mass flow was higher for the jars of 350 mL. After analyzing individual flows, it was found that in both cycles (polypropylene jars of 200 and 350 mL),the consumption of fresh water was a dominant flow. This fresh water flow is mostly (95%) consumed in the injection molding process of manufacturing jars from polypropylene granules. The LCA analysis showed no significant difference in global warming potential between different volume jars. The process that mostly affected global warming was the production of polypropylene jars from polypropylene granules by injection molding for both jar volumes. Judging by the global warming potential, there is no difference of the environmental impact between investigated jars, but considering the mass flow and water consumption, more environmental friendly were the 200 mL jars.

This research was conducted in order to examine the mass transfer kinetics, during osmotic dehydration of celery leaves (Apium graveolens) in two different osmotic solutions (sugar beet molasses and the mixed solution of sodium chloride and sucrose), under atmospheric pressure, at room temperature. The significance of used hypertonic solutions and immersion time on the various kinetics parameters (water loss, solid gain and dehydration efficiency index) were tested during the process, using Analysis of variance. Principal component analysis and Cluster analysis were used for characterization of different samples, and standard score analysis was applied in optimal process parameters determination.

In this paper, environmental impact of polyethylene tubes of different volumes: 30 mL and 100 mL was assessed using the life cycle assessment. The data were collected on the types and amounts of the materials and energy consumption in the process of packaging and distribution and inserted in the software GaBi 5. Total mass life cycle inventory was considerably lower for the smaller volume packaging. Breaking the inventory to the single flows, it was found that in both life cycles (polyethylene tubes of 30 mL and 100 mL) the highest contribution had the flows of fresh water consumption, emission of inorganic elements to the air and fresh water pollution with waste water flows. The highest contribution to all of these flows was shown to be production and consumption of diesel fuel. The results of this paper show that in analised case, more environmental friendly is the larger volume packaging.

This paper investigates the intensity of the influence which poly (ethylene oxide) and poly(ethylene glycol) additions have on the mechanical and structural properties of chitosan films. The films based on highly viscous and medium viscous chitosan were produced under laboratory conditions. Poly(ethylene oxide), with the average molecular weight of 100 000, and poly (ethylene glycol), with the average molecular weight of 400, were added to the films. The infrared spectrums of produced films were obtained on the FT-IR spectrometer and mechanical properties including: tensile strength (MPa) and elongation at break (%) were tested. The results show that the addition of poly(ethylene oxide) and poly(ethylene glycol) significantly affect the mechanical properties of chitosan films. The flexibility (expressed as the elongation at break) has increased more than five times, but there was a parallel decrease in film strength (expressed as tensile strength). The film based on highly viscous chitosan and poly(ethylene glycol) shows the best mechanical properties.

The aim of this study is optimisation of osmotic drying treatment of crucian carp (Carassius carassius) performed in sugar beet molasses solutions under atmospheric pressure, at constant solution temperature of 35°C. Statistical analysis was performed using StatSoft Statistica, for Windows, ver.10 program. Response surface methodology (RSM) approach was used for developing mathematical models describing the effects of the independent variables on the responses for dried fish samples. The independent variables were solution concentration (60, 70 and 80% w/w) and immersion times (1, 3 and 5h). Fuzzy synthetic evaluation was used to determine the optimum drying conditions. The optimum conditions appear to be treatment time of 5h and solution concentration of 80% w/w. The responses for the optimum drying conditions were found to be: dry matter content (DM) 62.59%, water loss (WL) 0.54g/g of initial sample weight, solid gain (SG) 0.10 of initial sample weight and water activity (aw) of 0.86.

This study investigated the impact of climate conditions on the quality of essential oils extracted from Apiaceae fruits, specifically coriander (Coriandrum sativum var. microcarpum), aniseed (Pimpinella anisum), and annual caraway (Carum carvi var. annuum) grown at three distinct locations in Serbia over three consecutive years. Field experiments were conducted, and essential oils were extracted using a Clevenger-type apparatus followed by gas chromatographic–mass spectrometric analysis for compound identification. Weather conditions during the vegetation periods were recorded, and statistical analyses, including principal component analysis (PCA) and correlation analysis, were performed to assess the volatile compound compositions. Results indicate significant correlations among various compounds within each fruit type, with distinct patterns observed across different years. PCA further elucidates the influence of both year and sampling location on the chemical profiles of essential oils. Cluster analysis reveals clustering primarily based on the year of cultivation rather than geographical location, emphasizing the dominant role of weather conditions in shaping essential oil compositions. This study highlights the intricate relationship between climate conditions and the quality of essential oils in Apiaceae fruits, providing valuable insights for optimizing cultivation practices and enhancing essential oil production. In general, climate conditions strongly influence the coriander, anise, and annual caraway cultivation, and also essential oil quality. [ABSTRACT FROM AUTHOR]

In this study, beetroots were osmotically dehydrated in sugar beet molasses. The input parameters of the drying process were varied: temperature (20 °C, 40 °C, and 60 °C), time (1 h, 3 h, and 5 h), and concentration of sugar beet molasses (40%, 60%, and 80%). Basic quality indicators were determined for the dried beetroot samples: dry matter content, water loss, solid gain, mineral and betaine content, and phenols and flavonoids, as well as antioxidant potential. After optimizing the results, favorable drying parameters were selected: temperature 60 °C, molasses concentration 70%, and processing time 5 h. According to the optimal drying conditions, the beetroots were dried and stored at 4 °C for 28 days. Half of the dried samples were coated with an edible biopolymer coating based on Camelina sativa oilcake, while the other half of the samples remained uncoated. The sustainability study aimed to confirm the effects of the biopolymer coating on the quality and sustainability of the osmotically dried beetroots. [ABSTRACT FROM AUTHOR]