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The objective of the current study was to evaluate Low-level laser therapy (LLLT) on the healing of incisional wounds following ovariohysterectomy in rats, by means of subjective histopathological and immunohistochemical analysis. A total of 72 female Wistar rats were categorised into four treatment groups (Group I; sacrification 4 hours following only one LLLT application, Group II; sacrification 7 days following only one LLLT application, Group III; sacrification 4 hours after two LLLT applications, and Group IV; sacrification 7 days after two LLLT applications). Each group was further divided into four different doses subgroups (Group Control [C, off mode LLLT application], L1 [1 J/cm2], L3 [3 J/cm2], and L6 [6 J/cm2]), with equal representation in each subgroup. Ovariohysterectomy was employed using two 2-cm-length midline abdominal incisions in the left and right sides of line alba. The Group C was assigned to the left side incision to each rat in the study. After irradiation, the tissue was subjected to histopathological analysis to determine the extent of mononuclear cell infiltration, edoema, and epithelialization. Additionally, immunohistochemical analysis was performed to evaluate the expression of proliferating cell nuclear antigen (pCNA) and inducible nitric oxide synthase (iNOS). Group L1 and L3 significantly decreased mononuclear cell infiltration compared with Group C in all treatment groups (p < 0.05). Group L3 significantly decreased edoema compared with Group C in all groups except for treatment Group I (p < 0.05). Group L2 and L3 significantly increased epithelization in treatment Group IV (p < 0.05). Moreover, Group L2 and L3 significantly increased pCNA in all groups, while L2 and L3 significantly decreased iNOS expression in treatment Group II, III, and IV (p < 0.05). However, no statistical difference was found between subgroups of treatment Group I in iNOS expiration (p > 0.05). The results of the current examination demonstrated that LLLT can modulate mononuclear cell infiltration and edoema, and improve epithelization, as well as increase pCNA expression, whereas decrease iNOS expression during the wound healing process, therefore enhancing wound healing following ovariohysterectomy in rats. [ABSTRACT FROM AUTHOR]

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The purpose of this study was to evaluate the effect of reuse of progesterone-releasing intravaginal devices (PRIVDs) on serum progesterone, estrogen levels, and ovulatory follicle diameter in pubertal heifers. Three pubertal Holstein heifers were used in the study for three different periods with the usage of three PRIVDs up to three times from day 0 to 7 according to the Latin square model. Blood samples were collected on days 0, 3, 5, 7, and 9 for hormonal analysis. Ovulatory follicle diameter was measured by transrectal ultrasonography 60 h after removal of the PRIVD. Serum progesterone demonstrated a significant relationship with device use and day (P < 0.01) with the 1st use group showing the highest mean serum progesterone levels. Mean serum progesterone levels of the 2nd and 3rd use did not show any significant difference among them. The peak level of mean serum progesterone was recorded on day 3 of the study, whereas minimum levels were seen at days 0 and 9. Serum progesterone concentrations of days 5 and 7 had no significant differences among them. The interaction between use of PRIVDs and days interval was significant (P < 0.01). Serum estrogen concentration (P > 0.05) and ovulatory follicle diameter (P > 0.05) did not differ significantly with the use of the PRIVD at day 9. In the present study, even the 3rd use of the PRIVD produced similar results as the first use. In conclusion, PRIVDs can be reused or manufactured with low progesterone for heifers to reduce hormonal synchronization cost. [ABSTRACT FROM AUTHOR]

Optical density image (ODI) analysis, based on the intensity of pixels in digital images, is commonly used in orthopedic medicine. This study was conducted to quantify endometrial and luteal changes in early pregnant and cyclic cows by ODI analysis. Twenty healthy primiparous Simmental cows on days 80-120 postpartum were subjected to ultrasonography and ODI analysis on the days of estrus (day 0) and metestrus (day 4) as well as 12, 15, 18, and 21 days after insemination. The ODI values of pregnant (P) and nonpregnant (NP) cows were compared using two-way ANOVA. The P cows tended to have greater endometrial OD values than NP cows (772.2 vs. 740.5, P < 0.08), but their luteal OD values were similar (596.0 ± 24). Both endometrial and luteal OD values varied over time in different patterns in P and NP cows in relation to gestational events until confirmation. In conclusion, preliminary data, especially endometrial OD values on day 15 relative to insemination, could have prognostic merit for a very early detection or confirmation of pregnancy. ODI analysis can be incorporated into ultrasonographic examination. However, more comprehensive experiments are needed to assure the prognostic value of ODI analysis. [ABSTRACT FROM AUTHOR]

The aim of this retrospective study was to evaluate the effects of breed, parity, and season on the reproductive parameters in bitches reared under a temperate climate. Breeding and pregnancy statuses, proestrus, estrus, and pregnancy durations, litter size, and cycle interval were recorded from German shepherds (GS, n = 34), Labrador retrievers (LA, n = 23), Belgian Malinois dogs (BM, n = 13), and Pointers (PO, n = 9) up to 10th parities. The mean age at first breeding (464.8 ± 26.2 days, mean ± SD) and the pregnancy rate (74.6%) were not different among breeds. The pregnancy duration was shortened as the number of puppies born increased in each whelping (P < 0.008). The cycle interval varied by breed (208.2, 215.1, 208.6, and 237.0 days for GS, LA, BM, and PO, respectively, P < 0.01) and decreased linearly from 241.1 to 202.0 days as parity increased from 1 to 10 (P < 0.04). The season did not affect the cycle interval, proestrus, and estrus length (P > 0.05). Reproductive parameters varied among the GS, LA, BM, and PO bitches reared in Turkey. The effect of seasonal variation was negligible. [ABSTRACT FROM AUTHOR]

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to investigate the compatibility of the visual evaluation result of the blood flow characteristics and the blood flow measurements of the CL and the predictability of the responses given by corpora lutea with varying levels of blood flow to an induction of luteolysis by a PGF 2α injection and to determine the possibility of increase in serum estrogen and progesterone concentrations in parallel with increased luteal blood flow (LBF). The cows, bearing a CL (n = 60; postpartum 35 days), were injected with PGF 2α and were monitored for signs of estrous following the first injection. The cows, which did not show estrous signs, were examined for the presence of a CL on Day 14, whereas those that showed signs of estrous were examined on Day 10 following the onset of estrous. The level of LBF was visually graded as + (low; GI), ++ (medium; GII), +++ (high; GIII), and ++++ (very high; GIV). Immediately after the examination of LBFs, a second intramuscular injection of PGF 2α was injected. In the cows, which were determined to be in estrous, the diameter of the Graafian follicles was measured by B-mode ultrasonography. Subsequently, these animals were artificially inseminated. The animals, which did not show estrous after the second injection, were examined as previously described and monitored for signs of estrous. A strong correlation (r = 0.654; P < 0.001) was determined to exist between the results of the visual examination of the images and the results obtained for the LBF area with the use of the Pixel Flux software. GIII (0.83 ± 0.15 cm 2 ) and GIV (1.03 ± 0.48 cm 2 ) were found to differ from GI (0.47 ± 0.23 cm 2 ) and GII (0.51 ± 0.12 cm 2 ) for the size of the LBF (P < 0.001). Serum progesterone levels in groups (GI, GII, GIII, and GIV) were determined to be 4.44 ± 2.42 ng/mL, 6.03 ± 2.37 ng/mL, 7.01 ± 2.94 ng/mL, and 7.17 ± 1.69 ng/mL, respectively. The comparative evaluation of the study groups showed that the groups did not statistically differ for the period between PGF 2α injection and the onset of estrous, mean Graafian follicle size and estrogen levels. No direct correlation existed between these reproductive parameters and LBF. [ABSTRACT FROM AUTHOR]