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Pregnancy and childbirth are uncertain experiences that become even more so when parents receive an unexpected medical diagnosis for their child. In the present study, we document sources of uncertainty and the tools used to manage uncertainty in 44 mothers' narratives about the birth and diagnosis of their child with Down syndrome (DS); we also explore variability in the sources of uncertainty and uncertainty management tools as a function of whether mothers received a prenatal or postnatal diagnosis of DS for their child. Across our sample, thematic analysis revealed four sources of uncertainty in mothers' narratives: navigating dissonance between parents and providers during diagnosis, managing disclosure of the diagnosis to others, anticipating child-centered challenges and adjusting developmental expectations, and anticipating family-centered challenges and adjusting familial expectations. Analyses also revealed four ways that uncertainty was managed: finding balance between parents and providers during diagnosis, reducing knowledge gaps by seeking information, pursuing support and building positive interpersonal relationships, and pursuing support and building positive relationships in DS communities. These findings, along with potential nuance based on whether mothers received a prenatal or postnatal diagnosis of DS for their child, have important implications for healthcare providers and medical professionals regarding how to best communicate DS diagnoses to families as a means of understanding and ultimately reducing the uncertainty they experience. [ABSTRACT FROM AUTHOR]

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Background: Depression and anxiety are common psychological issues among international medical students in universities, which have serious negative impacts on their learning and life. The present study aimed to evaluate the efficacy of positive emotional writing in alleviating symptoms of anxiety and depression, as well as enhancing subjective well-being among this population. Method: This study was conducted at an international college of a comprehensive university in central China. A total of ninety-two participants who met the inclusion criteria were recruited to participate in a single-blind randomized controlled trial, in which participants were not aware that there was an experimental group and a control group. Participants in control received daily psychological care weekly for 8 weeks (n = 46). Correspondingly, participants in experimental group received the positive emotional writing intervention on the basis of daily psychological care. Self-Rating Depression Scale (SDS), Self-Rating Anxiety Scale (SAS) and General Well-being Scale (GWB) were used to evaluate the effect of the intervention. Data from 89 students who completed the entire study (experimental group, n = 44; control group, n = 45) were analyzed. Results: After the intervention, the scores of SDS and SAS in the experimental group significantly decreased, while the subjective well-being score significantly increased. Although the SAS score of the control group after intervention was significantly lower than before, the decrease in SDS and SAS scores, as well as the increase in GWB score, were significantly greater in the experimental group than in the control group. Conclusion: The findings suggest that positive emotional writing can effectively reduce the depression and anxiety of international medical students, and significantly enhance their subjective well-being, providing ideas for management to solve the psychological problems of international medical students. Trial registration number: ChiCTR2400087815. Registered at Chinese Clinical Trial Registry. [ABSTRACT FROM AUTHOR]

Introduction: As the average global temperature increases, the effects of climate change worsen, through effects on worsening extreme events as well as exacerbating political, economic, and social turmoil (wars, conflicts, and migrations). This poses an existential risk to the survival of humans and nonhumans. These effects are visible due to the impact on people’s mental health and psychophysical well-being. This article aims to explore the growing phenomenon of psychoterratic syndromes, with focus on the effect of ecoanxiety on mental health. Furthermore, the relationship between eco-anxiety and behavior response (both individual and collective) in the climate crisis era is outlined. Methods: A research with interdisciplinary approach was carried out for recent literature and articles relating to psychoterratic syndromes and the effects of climate change on mental health. Results: The article explores the effects of climate change on mental health, including various research on the onset of new emotions in response to psychological effects to climate change, called psychoterratic syndromes (such as eco-anxiety, climate anxiety, solastalgia, eco-grief). Among these, eco-anxiety is the most popular term used for describing how people feel about climate change. However, the paradigm that described eco-anxiety only as a pathological emotion needs to be changed. Discussion: The article emphasizes the positive effect of eco-emotions and the need to stimulate people to move from a state of anxiety, which could bring apathy and resignation, toward eco-hope. Eco-hope could be an adaptive coping mechanism in people and communities, which is key to preventing, mitigating, and protecting mental and planetary health. [ABSTRACT FROM AUTHOR]

The study aimed to compare the quality of life (QoL) in two communities with different exposures to conflict and investigate the inter-relationship between posttraumatic stress disorder (PTSD), social support, and QoL. This is a cross-sectional with 413 participants. Study instruments included the PTSD module of the Composite International Diagnostic Interview (CIDI), the World Health Organization Quality of Life BREF (WHOQoL-BREF), and the Multidimensional Scale of Perceived Social Support (MSPSS). The family domain of social support was protective of both PTSD and QoL. Except for the relationship between community's location and the physical subscale of the QoL, a hierarchical regression analysis showed that all the independent variables were significantly associated with the QoL domains. Direct exposure to crises impaired QoL more than areas indirectly exposed. PTSD and the family domain of social support play a significant role in the QoL outcome. This suggests that therapeutic intervention to improve QoL should target these key variables. [ABSTRACT FROM AUTHOR]

Introduction: Food marketing restrictions often apply nutrient profile models (NPM) to distinguish unhealthy products that should not be advertised, however brand-only marketing remains largely unaddressed. We sought to test a threshold method for classifying packaged food, beverage, or fast-food brands as (non)permitted for marketing, based on the nutrient profile of their product-lines. Methods: We retrieved nutrient information from the Nutritrack databases for all products sold by the top 51 packaged food, beverage and fast-food brands in New Zealand, selected by market share. All products under each brand were classified as permitted (or not) to be marketed to children, using the NPM for WHO Western Pacific. The 25%, 50%, 75% and 90% threshold of brands' products permitted to market were compared. The 50% and 75% thresholds were compared to the WHO CLICK method, which is based on assessment of the brand's leading product. Results: The 90% threshold permitted 13% of the brands to be marketed to children. The 25% threshold permitted the marketing of 62% of brands. The 50% and 75% thresholds remained highly sensitive in identifying brands that should not be marketed to children. Comparison to the WHO CLICK method identified that a threshold method is more comprehensive and less arbitrary. Conclusions: A threshold model based on product-line nutrient profiling provides a robust and option for brand classification. The 50% and 75% thresholds may be the most politically preferred options for use in regulation, while remaining highly effective. Practitioner points: Brand marketing (e.g. sponsorship) remains largely unaddressed in existing restrictions on unhealthy food and beverage marketing to children. An established Nutrient Profile Model can be applied to a brand's entire product line, allowing calculation of the proportion of products that would not be permitted to be advertised to children. Restricting brand marketing for food and beverage brands with less than 50% or 75% of their products classified as 'permitted to be marketed to children' is a robust and evidence-based method that can be applied in regulation, with potential to mitigate industry challenges. [ABSTRACT FROM AUTHOR]

This study aims to identify the conceptualisation of overall well-being used for well-being assessment through a review of the characteristics and key components and/or dimensions of well-being scales as presented in current literature. Scopus and Web of Science were searched, and thematic analysis was conducted inductively to analyse the identified components within scales, as well as the types of well-being these scales measure. 107 peer-reviewed articles from 2003 to 2022 were included, and 69 well-being scales were identified covering nine areas of well-being. Four final themes were identified as the foundational dimensions of overall well-being: hedonic; eudaimonic; physical health; and generic happiness. Notably, these 69 scales are mainly validated and adopted in the Western context. '4 + N' frameworks of overall well-being are recommended for assessing overall well-being. This review provides researchers with a synthesis of what types of well-being have been measured and which measures have been used to assess these types of well-being for which research participants. Non-Western-based well-being research is called for that incorporates a broader range of research participants and cultural contexts in contributing to a more inclusive understanding of well-being. [ABSTRACT FROM AUTHOR]

Background: Unstable gait leading to falls negatively impacts the quality of life in many people with Parkinson's disease (PD). Systematic review evidence provides moderate to strong evidence of efficacy for a wide range of physiotherapybased interventions to reduce gait impairment. However, outcomes have often focused on gait assessments conducted in controlled laboratory or clinical environments. Objective: This perspective investigates the complexities and challenges of conducting real-world gait assessments in people with PD and the factors that may influence the translation from improved lab-assessed gait to improved real-world gait. Methods: Through a thorough review of current literature, we present an in-depth analysis of current methodological approaches to real-world gait assessments and the challenges that may influence the translation of an intervention's success from lab-based outcomes to improved walking during daily life. Results: We identified six key factors that may influence the translation of intervention success into real-world environments at different stages of the process. These factors comprise the gait intervention, parameters analyzed, sensor setup, assessment protocols, characteristics of walking bouts, and medication status. We provide recommendations for each factor based on our synthesis of current literature. Conclusion: This perspective emphasizes the importance of measuring intervention success outside of the laboratory environment using real-world gait assessments. Our findings support the need for future studies to bridge the gap between proven efficacy for gait as assessed in controlled laboratory environments and real-world impact for people with PD. [ABSTRACT FROM AUTHOR]

In the developing brain, groups of neurons organize into functional circuits that direct diverse behaviors. One such behavior is the evolutionarily conserved acoustic startle response, which in zebrafish is mediated by a well-defined hindbrain circuit. While numerous molecular pathways that guide neurons to their synaptic partners have been identified, it is unclear if and to what extent distinct neuron populations in the startle circuit utilize shared molecular pathways to ensure coordinated development. Here, we show that the planar cell polarity (PCP)-associated atypical cadherins Celsr3 and Celsr2, as well as the Celsr binding partner Frizzled 3a/Fzd3a, are critical for axon guidance of two neuron types that form synapses with each other: the command-like neuron Mauthner cells that drive the acoustic startle escape response, and spiral fiber neurons which provide excitatory input to Mauthner cells. We find that Mauthner axon growth towards synaptic targets is vital for Mauthner survival. We also demonstrate that symmetric spiral fiber input to Mauthner cells is critical for escape direction, which is necessary to respond to directional threats. Moreover, we identify distinct roles for Celsr3 and Celsr2, as Celsr3 is required for startle circuit development while Celsr2 is dispensable, though Celsr2 can partially compensate for loss of Celsr3 in Mauthner cells. This contrasts with facial branchiomotor neuron migration in the hindbrain, which requires Celsr2 while we find that Celsr3 is dispensable. Combined, our data uncover critical and distinct roles for individual PCP components during assembly of the acoustic startle hindbrain circuit. Author summary: The assembly of neuronal circuits that drive behavior requires coordination of molecular pathways that govern neuron development and connectivity. Disruption of circuit development can lead to behavioral dysfunction, but understanding how circuits develop in vivo, especially in the immensely complex human brain, poses a significant challenge. Here, we leverage a well-characterized circuit driving a conserved protective behavior, the acoustic startle response, in the developing zebrafish to investigate molecular pathways driving circuit assembly. We discovered that proteins in the planar cell polarity (PCP) pathway are critical for development of two neuron types in the startle hindbrain circuit: the large paired "Mauthner neurons," which mediate the acoustic startle fast escape, and a group of specialized Mauthner-activating "spiral fiber neurons." We found that the PCP atypical cadherin Celsr3 guides Mauthner axon growth towards and into the spinal cord, where Mauthner axons form connections with motor neurons essential for the startle response. Additionally, we uncovered unique and overlapping roles for Celsr3 and the related Celsr2 protein in hindbrain circuit development, shedding light on their specific functions in neurodevelopment. Overall, our findings in zebrafish illustrate how the conserved PCP pathway orchestrates circuit assembly and influences behavior. [ABSTRACT FROM AUTHOR]

Introduction: The clinicopathological characteristics and prognosis of placental site trophoblastic tumor (PSTT) and epithelioid trophoblastic tumor (ETT) have not been well summarized. Consequently, we conducted the largest to date series of samples of both types and employed machine learning (ML) to assess treatment effectiveness and develop accurate prognostic models for patients with GTN. Gestational choriocarcinoma (GCC) was used as the control group to show the clinical features of PTSS and ETT. Methods: The Surveillance, Epidemiology, and End Results (SEER) database provided the data used for this study's analysis. To identify the prognostic variables, we conducted Cox regression analysis and constructed prognostic models using five ML algorithms to predict the 5-year survival. A validation method incorporating the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was used to validate the accuracy and reliability of the ML models. We also investigated the role of multiple therapeutic options using the Kaplan-Meier survival analysis. Results: The study population comprised 725 patients. Among them, 139 patients had ETT, 107 had PSTT, and 479 had GCC. There were no significant differences in survival between the different tumor groups. Multivariate Cox regression analysis revealed that metastasis was a significant prognostic factor for GCC, while older age and radiotherapy were significant prognostic factors for PTSS and ETT. ML models revealed that the Gradient Boosting classifier accurately predicted the outcomes, followed by the random forest classifier, K-Nearest Neighbors, Logistic Regression, and multilayer perceptron models. The most significant contributing factors were tumor size, year of diagnosis, age, and race. Discussion: Our study provides a method for treatment and prognostic assessment of patients with GTN. The ML we developed can be used as a convenient individualized tool to facilitate clinical decision making. [ABSTRACT FROM AUTHOR]

The mindful state is commonly characterized by an elevated awareness of the present moment. An intriguing and rather widespread phenomenon that requires the attention to the present moment is Synchronicity Awareness. This phenomenon refers to the sense of a profound psychological connection between an internal event (e.g., thought, image, or dream) and external events. Whereas mindfulness and its underlying cognitive processes have been well documented, synchronicity awareness, despite its deep roots, has been scarcely examined empirically, and little is known about the cognitive mechanisms underlying it. The present study aimed to further validate the recently developed Synchronicity Awareness and Meaning Detection (SAMD) scale and explore its potential relationship with various mindfulness measures. To this end, 572 participants engaged in an online survey, incorporating the SAMD, Five Facets of Mindfulness Questionnaire, Mindful Awareness and Attention Scale, Langer's Mindfulness Scale, and Encoding Style Questionnaire. The results revealed that synchronicity awareness, meaning detection, and mindfulness are distinct constructs. A significant association between synchronicity and core facets of mindfulness was observed, indicating that participants with higher SAMD scores were more sensitive to inner sensations, more novelty-seeking, and engaged in their actions. Additionally, SAMD scores were linked to internal encoding style, suggesting a top-down processing of experiences. However, synchronicity was not associated with outwards-directed mindful awareness, suggesting that it might involve an intrinsic attentional process, influenced by internal cues. Theoretical and practical implications are discussed. [ABSTRACT FROM AUTHOR]

Ethical conflicts (ECs), dilemmas auditors face when personal values or professional obligations clash with their actions, pose significant challenges to the auditing profession, potentially influencing turnover intention (TI). This study addresses a knowledge gap in the related research by focusing on two critical EC triggers: workload (WL) and perceived auditor ethical failure (PAEF: ethical sensitivity), which refers to auditors' perceptions of ethical violations within their profession. Grounded in role theory and ethical climate theory, our study investigates the impact of WL and PAEF on ECs and explores how these conflicts mediate the relationship between PAEF/WL and TI. Additionally, we examine the moderating role of organisational ethical climate (EtC) in these relationships. Using a survey of auditors and employing partial least squares structural equation modelling (PLS-SEM), our results reveal that both WL and PAEF increase ECs, thus escalating TI. The findings suggest that a firm's perceived EtC can negatively moderate the relationship between PAEF and ECs. The study underlines the importance of fostering a strong EtC to manage ECs effectively and provides valuable implications for professionals, regulators, and academics seeking to improve ethical practices within auditing firms and to reduce auditor turnover. [ABSTRACT FROM AUTHOR]

Classification of pathogenic E. coli has been focused either in mammalian host or infection site, which offers limited resolution. This review presents a comprehensive framework for classifying all E. coli branches within a single, unifying figure. This approach integrates established methods based on virulence factors, serotypes and clinical syndromes, offering a more nuanced and informative perspective on E. coli pathogenicity. The presence of the LEE island in pathogenic E. coli is a key genetic marker differentiating EHEC from STEC strains. The coexistence of stx and eae genes within the bacterial genome is a primary characteristic used to distinguish STEC from other pathogenic E. coli strains. The presence of the inv plasmid, Afa/Dr adhesins, CFA-CS-LT-ST and EAST1 are key distinguishing features for identifying pathogenic E. coli strains belonging to EIEC, DAEC, ETEC and EAEC pathotypes respectively. Food microbiological criteria differentiate pathogenic E. coli in food matrices. 'Zero-tolerance' applies to most ready-to-eat (RTE) foods due to high illness risk. Non-RTE foods' roles may allow limited E. coli presence, which expose consumers to potential risk; particularly from the concerning Shiga toxin-producing E. coli (STEC) strains, which can lead to life-threatening complications in humans, including haemolytic uremic syndrome (HUS) and even death in susceptible individuals. These findings suggest that decision-makers should consider incorporating the separate detection of STEC serotypes into food microbiological criteria, in addition to existing enumeration methods. Contamination of STEC is mainly linked to food consumption, therefore, outbreaks of E. coli STEC has been reviewed here and showed a link also to water as a potential contamination route. Since their discovery in 1982, over 39,787 STEC cases associated with 1,343 outbreaks have been documented. The majority of these outbreaks occurred in the Americas, followed by Europe, Asia and Africa. The most common serotypes identified among the outbreaks were O157, the 'Big Six' (O26, O45, O103, O111, O121, and O145), and other serotypes such as O55, O80, O101, O104, O116, O165, O174 and O183. This review provides valuable insights into the most prevalent serotypes implicated in STEC outbreaks and identifies gaps in microbiological criteria, particularly for E. coli non-O157 and non-Big Six serotypes. [ABSTRACT FROM AUTHOR]

Purpose: Cover crops and reduced tillage are two key climate smart agricultural practices that can provide agroecosystem services including improved soil health, increased soil carbon sequestration, and reduced fertilizer needs. Crop residue carbon traits (i.e., lignin, holocellulose, non-structural carbohydrates) and nitrogen concentrations largely mediate decomposition rates and amount of plant-available nitrogen accessible to cash crops and determine soil carbon residence time. Non-destructive approaches to quantify these important traits are possible using spectroscopy. Methods: The objective of this study was to evaluate the efficacy of spectroscopy instruments to quantify crop residue biochemical traits in cover crop agriculture systems using partial least squares regression models and a combination of (1) the band equivalent reflectance (BER) of the PRecursore IperSpettrale della Missione Applicativa (PRISMA) imaging spectroscopy sensor derived from laboratory collected Analytical Spectral Devices (ASD) spectra (n = 296) of 11 cover crop species and three cash crop species, and (2) spaceborne PRISMA imagery that coincided with destructive crop residue collections in the spring of 2022 (n = 65). Spectral range was constrained to 1200 to 2400 nm to reduce the likelihood of confounding relationships in wavelengths sensitive to plant pigments or those related to canopy structure for both analytical approaches. Results: Models using laboratory BER of PRISMA all demonstrated high accuracies and low errors for estimation of nitrogen and carbon traits (adj. R2 = 0.86 − 0.98; RMSE = 0.24 − 4.25%) and results indicate that a single model may be used for a given trait across all species. Models using spaceborne imaging spectroscopy demonstrated that crop residue carbon traits can be successfully estimated using PRISMA imagery (adj. R2 = 0.65 − 0.75; RMSE = 2.71 − 4.16%). We found moderate relationships between nitrogen concentration and PRISMA imagery (adj. R2 = 0.52; RMSE = 0.25%), which is partly related to the range of nitrogen in these senesced crop residues (0.38–1.85%). PRISMA imagery models were also influenced by atmospheric absorption, variability in surface moisture content, and some presence of green vegetation. Conclusion: As spaceborne imaging spectroscopy data become more widely available from upcoming missions, crop residue trait estimates could be regularly generated and integrated into decision support tools to calculate decomposition rates and associated nitrogen credits to inform precision field management, as well as to enable measurement, monitoring, reporting, and verification of net carbon benefits from climate smart agricultural practice adoption in an emerging carbon marketplace. [ABSTRACT FROM AUTHOR]

This study assesses the potential of using multispectral images collected by an unmanned aerial system (UAS) on machine learning (ML) frameworks to estimate cereal rye (Secale cereal L.) biomass. Multispectral images and ground-truth cereal rye biomass data were collected from 15 farmers' fields up to three times between March and May in northwest Ohio. Images were processed to derive 13 vegetation indices (VIs). Out of 13 VIs, six optimal sets of VIs, including excess green (ExG), normalized green red difference index (NGRDI), soil adjusted vegetation index (SAVI), blue green ratio (B_G_ratio), red-edge triangular vegetation index (RTVI), and normalized difference red-edge (NDRE) were selected using the variance inflation factor (VIF) based feature selection approach. Six regression models including a multiple linear regression (MLR), elastic net (ENET), multivariate adaptive regression splines (MARS), support vector machine (SVM), random forest (RF), and extreme gradient boosting (XGB) were investigated for estimation of cereal rye biomass based on the VIs. For most of the models, the six selected VIs performed better than or similar to the full set of 13 VIs with R2 ranging from 0.24 to 0.59 and RMSE ranging from 83.13 to 91.89 g/m2 during 10-fold cross-validation. During independent accuracy assessment with the selected set of VIs, XGB exhibited the highest R2 (0.67) and lowest RMSE (83.13 g/m2) and MAE (48.13 g/m2) followed by RF and ENET. For all the models, the agreement between observed and predicted biomass was high for biomass less than or equal to 200 g/m2 but decreased for biomass greater than 200 g/m2. When field-collected structural features were integrated with the selected VIs, the models showed improved performance, with R2 and RMSE of the models reaching up to 0.82 and 61.67 g/m2 respectively. Among the six VIs, SAVI showed the strongest impact on the model prediction for the best-performing RF and XGB regression models. The findings of this study demonstrate the potential of precisely estimating and mapping cereal rye biomass based on UAS-captured multispectral images. Timely information on cover crop growth can facilitate numerous decision-making processes, including planning the planting operations, and management of nutrients, weeds, and soil moisture to improve agronomic and environmental outcomes. [ABSTRACT FROM AUTHOR]

We must urgently synthesize highly efficient and stable oxygen-evolution reaction (OER) catalysts for acidic media. Herein, we constructed a series of Ti mesh (TM)-supported RuO2/CoMoyOx catalysts (RuO2/CoMoyOx/TM) with heterogeneous structures. By optimizing the ratio of Co to Mo, RuO2/CoMo2Ox/TM with low Ru loading (0.079 mg/cm2) achieves remarkable OER performance (η = 243 mV at 10 mA/cm2) and high stability (300 h @ 10 mA/cm2) in 0.5 mol/L H2SO4 electrolyte. The activity of RuO2/CoMoyOx/TM can be maintained for 50 h at 100 mA/cm2, and a water electrolyzer with RuO2/CoMo2Ox/TM as anode can operate for 40 h at 100 mA/cm2, suggesting the remarkable OER durability of RuO2/CoMoyOx/TM in acidic electrolyte. Owing to the heterogeneous interface between CoMo2Ox and RuO2, the electronic structure of Ru atoms was optimized and electron-rich Ru was formed. With modulated electronic properties, the dissociation energy of H2O is weakened, and the OER barrier is lowered. This study provides the design of low-cost noble metal catalysts with long-term stability in an acidic environment. [ABSTRACT FROM AUTHOR]

Mindfulness is an increasingly popular practice among elite athletes to improve performance and well-being, but its application for coaches is still very limited. Therefore, a new program (M×E; Mindfulness para Entrenadores) was designed and implemented online for 6 weeks for coaches, support staff, and technical directors. Participants were 58 (57 men), aged between 23 and 58 (M = 31.8). The between-subject analysis (RM ANOVA 2×2) included experimental (n = 26) and wait-list control group (n = 21). A total of 29 completed the within-subject analysis (RM ANOVA) completing follow-up measurements until six months. The analysis was complemented with 21 individual semi-structured interviews. The experimental group showed significant improvements in mindfulness trait (p <.001), interpersonal mindfulness (p =.010), and a significant improvement in emotional regulation (p =.010) in comparison to the wait-list control group. The experimental group's positive trend in all variables' levels was maintained until six months after the program. The qualitative analysis showed a positive impact on professional, personal, and social areas with improvements related to performance and well-being. Several participants considered the program as "fundamental" for their professional role. This study provides information on best practices in implementing mindfulness-based programs, highlighting their practical orientation, the training plan, and the safe space. These results offer initial validation of the potential of the M×E and invite sports organizations to incorporate mindfulness-based programs specially designed for coaches, support staff and technical directors. [ABSTRACT FROM AUTHOR]

The innate immune system serves as the body's first line of defense, utilizing pattern recognition receptors like Toll‐like receptors to detect pathogens and initiate rapid response mechanisms. Following this initial response, adaptive immunity provides highly specific and sustained killing of pathogens via B cells, T cells, and antibodies. Traditionally, it has been assumed that innate immunity activates adaptive immunity; however, recent studies have revealed more complex interactions. This review provides a detailed dissection of the composition and function of the innate and adaptive immune systems, emphasizing their synergistic roles in physiological and pathological contexts, providing new insights into the link between these two forms of immunity. Precise regulation of both immune systems at the same time is more beneficial in the fight against immune‐related diseases, for example, the cGAS–STING pathway has been found to play an important role in infections and cancers. In addition, this paper summarizes the challenges and future directions in the field of immunity, including the latest single‐cell sequencing technologies, CAR‐T cell therapy, and immune checkpoint inhibitors. By summarizing these developments, this review aims to enhance our understanding of the complexity interactions between innate and adaptive immunity and provides new perspectives in understanding the immune system. [ABSTRACT FROM AUTHOR]

Organizational and family support may be invested to reduce workers' family-to-work conflict and increase their and their family members' family satisfaction. However, data on the extent to which workplace and family support, family-to-work conflict, and family satisfaction were linked during the COVID-19 pandemic for workers and their families is still limited. This study explored the actor and partner effects between perceived workplace support for families and perceived family support, family-to-work conflict, and family satisfaction, in different-sex dual-earner parents with adolescents and the mediating role of parents' family-to-work conflict. In the Metropolitan Region of Chile, 430 dual-earner parents and their adolescent child (mean age of 12.8 years, 53.7% female) were recruited for the study. Mothers and fathers responded to measures of family-to-work conflict and perceived workplace support for families and the Perceived Family Support Scale. The three family members answered the Satisfaction with Family Life Scale. Analyses were conducted using the Actor-Partner Interdependence Model and structural equation modeling. Results showed that mothers' and fathers' perceived workplace support for families and perceived family support helped reduce their family-to-work conflict; no other association involving parents' perceived workplace support for families was significant. By contrast, direct actor and partner effects were found between parents' perceived family support and the three family members' family satisfaction. Family-to-work conflict showed two significant mediating roles: One intraindividual in fathers and one intraindividual from fathers to adolescents. These findings indicate that resources both parents gain from perceived family support positively affect the three family members' family satisfaction by different mechanisms and underscore the importance of family support for family satisfaction during the pandemic. [ABSTRACT FROM AUTHOR]

Previous research has established a positive correlation between meaning in life and academic achievement, yet several gaps remain unaddressed. Firstly, the directionality of their relationships is unclear, particularly the potential impact of academic engagement on life meaning. Secondly, most studies have not distinguished between the distinct dimensions of meaning in life—presence of meaning and search for meaning. Thirdly, the underlying mechanisms of these relationships are not well-understood. To address these gaps, this study conducted a six-month, two-wave longitudinal analysis involving 3,895 Chinese secondary school students. Utilizing a cross-lagged panel model, we examined the causal relationships between both dimensions of meaning in life and academic engagement, with a specific focus on the mediating role of hope. Results indicated a reciprocal relationship between presence of meaning and academic engagement, where hope acted as a positive mediator within their bidirectional causal pathways. Conversely, the relationship between the search for meaning and academic engagement was unidirectional, with academic engagement significantly and positively predicting the search for meaning via increased levels of hope, but not vice versa. The study concludes with a discussion of research implications and future research directions. [ABSTRACT FROM AUTHOR]

The main objective of this study is to examine the psychometric properties of the PERMA-Profiler in a Spanish context. The PERMA-Profiler, developed by Butler & Kern (Int J Wellbeing 6(3):1-48, 2016) to measure Seligman's (Flourish: A visionary new understanding of happiness and well-being, 2011) PERMA model of flourishing, consists of five domains that assess well-being: Positive Emotion (P), Engagement (E), Relationships (R), Meaning (M), and Accomplishment (A). We translated and adapted the PERMA-Profiler, analyzed the instrument's reliability, its validity based on an internal structure through three confirmatory factor analyses, gender and age invariance, and its convergent and discriminant validity. A total of 2525 participants completed all measures. The results of the analyses to confirm the internal consistency are very acceptable in all the domains and in Overall Well-being (PERMA), except for the Engagement domain. The results of three confirmatory factor analyses show that the model of five independent interrelated factors (domains) presents the best fit. The analysis shows the invariance across gender and age groups. The analyses of the convergent validity show that are positively and significantly related to satisfaction with life (SWLS), to the six evaluated dimensions of psychological well-being (PWB), to positive affect (PANAS) and dispositional optimism (LOT-R) and the general physical and mental health status (SF-36). The discriminant validity analyses show that are related negatively and significantly to negative affect (PANAS), the total score of depression (BDI-II) and the Cognitive-Affective and Somatic-Motivational factors. The findings of this study indicate that the PERMA-Profiler is transferable to the Spanish context, and the Spanish version is a reliable and valid measure of well-being. [ABSTRACT FROM AUTHOR]

Objective: This study investigates patients' medication‐taking routines and the feasibility of harnessing habit formation through context cues and rewards to improve medication adherence. Methods: Semistructured qualitative interviews with patients with gout from an urban health care system were conducted to explore typical medication‐taking behavior, experiences using electronic pill bottles, barriers to adherence, existing context cues, and potential cues and rewards for habit‐forming behavior. Medication‐taking patterns were recorded for six weeks using electronic pill bottles before interviews to inform discussion. Transcribed interviews were analyzed to generate themes using codes developed by the study team, with representative quotations selected as illustrations. Results: We conducted interviews with 15 individuals (mean age 60.6 [SD 20.3] years, three women [20%], and nine White patients [60%]). Pill bottle–recorded adherence to urate‐lowering therapy (ULT) was high (mean 0.91 [SD 0.10]), and one patient was experiencing an active gout flare. Five key themes emerged: (1) reasons for nonadherence, (2) internal and external motivations for adherence, (3) structured routines around taking medications, (4) rewards for good medication adherence, and (5) the role of pill cap technology in medication‐taking. Conclusion: The importance of a predictable, structured routine in which participants could incorporate their medication‐taking behavior emerged as a key factor that promoted consistent adherence. Further, identifying context cues and reminders seemed to promote incorporation of medication‐taking into routines. Therefore, habit‐based interventions that use context cues to establish routines around medication‐taking may be a feasible strategy to improve adherence in patients with chronic conditions such as gout. [ABSTRACT FROM AUTHOR]

Background: Cannabis use can generate potential avoidable harms, hence the need for effective preventive measures and treatment. Studies show the efficacy of harm reduction (HR) in minimizing undesirable consequences associated with this use. Despite its proven efficacy, HR in cannabis use remains poorly applied by many health and social services (HSS) practitioners, especially with young people. However, knowledge regarding the underlying reasons for this is limited. To fill this gap, we aimed to identify facilitators of and obstacles to HSS practitioners' adoption of HR in cannabis use across OECD countries. Methods: We conducted a scoping review, guided by Arksey and O'Malley's model. The search strategy, executed on health databases and in the grey literature, captured 1804 studies, of which 35 were retained. Data from these studies were extracted in summary sheets for qualitative and numerical analysis. Results: Facilitators and obstacles were grouped into four themes: stakeholders' characteristics (e.g., education, practice experience); clients' characteristics (e.g., personal, medical); factors related to HR (e.g., perceived efficacy, misconceptions); factors related to the workplace (e.g., type of workplace). Data were also extracted to describe the populations recruited in the selected studies: type of population, clientele, workplace. Conclusion: Several factors might facilitate or hinder HSS practitioners' adoption of HR in cannabis use. Taking these into consideration when translating knowledge about HR can improve its acceptability and applicability. Future research and action should focus on this when addressing practitioners' adoption of HR. [ABSTRACT FROM AUTHOR]

Purpose: Distinguished from cuproptosis and ferroptosis, disulfidptosis has been described as a newly discovered form of non-programmed cell death tightly associated with glucose metabolism. However, the prognostic profile of disulfidptosis-related lncRNAs (DRLRs) in ovarian cancer (OC) and their biological mechanisms need to be further elucidated. Materials and methods: First, we downloaded the profiles of RNA transcriptome, clinical information for OC patients from the TCGA database. Generated from Cox regression analysis, prognostic lncRNAs were utilized to identify the risk signature by least absolute shrinkage and selection operator analysis. Then, we explored the intimate correlations between disulfidptosis and lncRNAs. What's more, we performed a series of systemic analyses to assess the robustness of the model and unravel its relationship with the immune microenvironment comprehensively. Results: We identified two DRLR clusters, in which OC patients with low-risk scores exhibited a favorable prognosis, up-regulated immune cell infiltrations and enhanced sensitivity to immunotherapy. Furthermore, validation of the signature by clinical features and Cox analysis demonstrated remarkable consistency, suggesting the universal applicability of our model. It's worth noting that high-risk patients showed more positive responses to immune checkpoint inhibitors and potential chemotherapeutic drugs. Conclusion: Our findings provided valuable insights into DRLRs in OC for the first time, which indicated an excellent clinical value in the selection of management strategies, spreading brilliant horizons into individualized therapy. [ABSTRACT FROM AUTHOR]

Background: This study uses a randomized controlled trial (RCT) to test the health benefits of expressive writing that is culturally adapted for Chinese immigrant breast cancer survivors (BCSs) and to characterize how acculturation moderates the effects of expressive writing interventions. Methods: We will recruit Chinese immigrant BCSs (N = 240) diagnosed with stage 0-III breast cancer and within 5 years of completion of primary treatment. Recruitment will occur primarily through community-based organizations and cancer registries. Participants will be randomly assigned either to a control condition to write about neutral topics or to one of two intervention conditions, self-regulation or self-cultivation, both of which aim to promote adaptive cognitive processes but differ in how they achieve this goal. The self-regulation intervention culturally adapts a Western expressive writing paradigm and incorporates emotional disclosure, whereas the self-cultivation intervention originates from Asian cultural values without disclosing emotions. Participants in all three conditions will be asked to write in their preferred language for three 30-minute sessions. The primary outcome will be quality of life (QOL) at the 6- and 12-month follow-ups, and the secondary outcomes will be perceived stress, stress biomarkers, and medical appointments for cancer-related morbidities. Discussion: This project will be the first large RCT to test culturally based brief interventions to improve QOL and reduce stress among Chinese immigrant BCSs. This project is expected to address two important needs of Chinese immigrant BCSs: their unmet psychological needs and the lack of culturally competent mental health care for Chinese immigrant BCSs. The immediate product of this line of research will be empirically evaluated, culturally responsive interventions ready for dissemination to Chinese immigrant BCSs across the United States. ClinicalTrials.gov identifier: NCT04754412. [ABSTRACT FROM AUTHOR]

Assessing the prognosis of patients with aggressive non-Hodgkin B cell lymphoma mainly relies on a clinical risk score (IPI). Standard first-line therapies are based on a chemo-immunotherapy with rituximab, which mediates CD16-dependent antibody-dependent cellular cytotoxicity (ADCC). We phenotypically and functionally analyzed blood samples from 46 patients focusing on CD16+ NK cells, CD16+ T cells and CD16+ monocytes. Kaplan-Meier survival curves show a superior progression-free survival (PFS) for patients having more than 1.6% CD16+ T cells (p = 0.02; HR = 0.13 (0.007–0.67)) but an inferior PFS having more than 10.0% CD16+ monocytes (p = 0.0003; HR = 16.0 (3.1-291.9)) at diagnosis. Surprisingly, no correlation with NK cells was found. The increased risk of relapse in the presence of > 10.0% CD16+ monocytes is reversed by the simultaneous occurrence of > 1.6% CD16+ T cells. The unexpectedly strong protective function of CD16+ T cells could be explained by their high antibody-dependent cellular cytotoxicity as quantified by real-time killing assays and single-cell imaging. The combined analysis of CD16+ monocytes (> 10%) and CD16+ T cells (< 1.6%) provided a strong model with a Harrell's C index of 0.80 and a very strong power of 0.996 even with our sample size of 46 patients. CD16 assessment in the initial blood analysis is thus a precise marker for early relapse prediction. Highlights: High CD16+ T cell counts have a positive correlation with PFS in aggressive NHL/DLBCL patients (p = 0.02; HR = 0.13, 0.01–0.7). High CD16+ monocyte counts have a negative correlation with PFS in aggressive NHL/DLBCL patients (p = 0.0003; HR = 16.0, 3-292). The combined assessment of CD16+ T cells and CD16+ monocytes accurately predicts PFS in aggressive NHL/DLBCL patients. The strong protective function of CD16+ T cells could be explained by their high antibody-dependent cellular cytotoxicity. [ABSTRACT FROM AUTHOR]

Meaning salience, or awareness of meaning in the present moment, is an emerging concept with potential implications for health and wellness. The purpose of this study was to understand the relationships between meaning salience and eight dimensions of wellness, and to see whether meaning salience moderated the relationship between presence of meaning and wellness. Participants (N = 1711) were recruited to complete a cross-sectional survey through Amazon's Mechanical Turk. Meaning salience was related to better wellness in seven of the eight wellness domains (healthy eating and activity, financial stress, occupational wellness, sleep, perceived wellness, social wellness, and stress, excluding avoiding unhealthy foods). Meaning salience significantly moderated the relationships between presence of meaning and four of eight wellness dimensions, such that at high levels of meaning salience, both high and low presence of meaning had stronger relationships with wellness. Those with high meaning salience and high presence of meaning scored best on four wellness dimensions; however, those with high meaning salience and low presence of meaning scored the worst on four wellness dimensions. Future research should explore what factors drive the relationship between meaning salience and wellness. [ABSTRACT FROM AUTHOR]

Background: Parkinson's disease is characterized by symptoms such as bradykinesia and rigidity, which worsen as the disease progresses, significantly impacting patients' independence and quality of life. This study utilizes a network meta-analysis approach to quantify information gathered from randomized controlled trials (RCTs) regarding motor interventions that effectively improve the motor function of Parkinson's disease patients, aiming to provide evidence for selecting appropriate exercise intervention strategies for patients. Methods: A systematic search strategy for randomized controlled trials (RCTs) restricted to English was constructed based on multiple biomedical databases. Databases searched included PubMed, Embase, Cochrane, Web of Science, CINAHL, CBM, China National Knowledge Infrastructure (CNKI), Wan fang, VIP, etc., with searches conducted from inception to July 9, 2023. Two authors screened all studies, extracted data, and used frequency domain analysis methods. Network meta-analysis was performed using STATA software version 18.0 to compare and rank exercises that could effectively improve the motor function of Parkinson's disease patients (measured by indicators such as MDS-UPDRS-III, TUG, BBS, Mini-BES Test, 6MWT scores). Additionally, a series of analyses and evaluations were conducted, such as assessing the methodological quality of included studies using the Cochrane risk of bias tool. Results: The network meta-analysis included a total of 111 studies involving 5,358 participants, 133 intervention experiments, and 31 intervention measures. Although most exercise interventions showed effectiveness, cumulative ranking curves under the surface (SUCRA) values showed that archery exercise significantly improved patients' MDS-UPDRS-III scores (SUCRA = 95.6%), significantly superior to routine care [standardized mean difference (SMD = 16.92, 95%CI = -28.97, -4.87)]. High-intensity and agility exercise (High strength and agility) referred to as high-intensity exercise or agility training or a combination of both, collectively termed as high-intensity agility training, significantly improved patients' completion time for the time-up-and-go test (SUCRA = 99.7%), (SMD = -7.88, 95%CI = -9.47, -6.28). Dance and Tai Chi exercises significantly improved patients' balance abilities: Mini-Balance Evaluation Systems Test (SUCRA = 77.9%), (SMD = 5.25, 95%CI = -0.42, 10.92) for dance intervention and Berg Balance Scale (SUCRA = 94.7%), (SMD = 11.22, 95%CI = 3.26, 19.18) for Tai Chi intervention. Dance also significantly improved patients' walking ability in the 6-min walk test (SUCRA = 80.5%), (SMD = 71.31, 95%CI = 13.77, 128.84). Conclusion: Compared to other exercises, archery, dance, Tai Chi, and high-intensity agility exercises demonstrate superior efficacy in improving the motor function of Parkinson's disease patients. [ABSTRACT FROM AUTHOR]

Bipolar membranes in electrochemical CO2 conversion cells enable different reaction environments in the CO2-reduction and O2-evolution compartments. Under ideal conditions, water-splitting in the bipolar membrane allows for platinum-group-metal-free anode materials and high CO2 utilizations. In practice, however, even minor unwanted ion crossover limits stability to short time periods. Here we report the vital role of managing ionic species to improve CO2 conversion efficiency while preventing acidification of the anodic compartment. Through transport modelling, we identify that an anion-exchange ionomer in the catalyst layer improves local bicarbonate availability and increasing the proton transference number in the bipolar membranes increases CO2 regeneration and limits K+ concentration in the cathode region. Through experiments, we show that a uniform local distribution of bicarbonate ions increases the accessibility of reverted CO2 to the catalyst surface, improving Faradaic efficiency and limiting current densities by twofold. Using these insights, we demonstrate a fully platinum-group-metal-free bipolar membrane electrode assembly CO2 conversion system exhibiting <1% CO2/cation crossover rates and 80-90% CO2-to-CO utilization efficiency over 150 h operation at 100 mA cm−2 without anolyte replenishment. Here, authors report that local ionic transport across cathode catalyst layers is vital in improving CO production from CO2. This work demonstrates the potential of a CO2 electrolyzer constructed from materials free from platinum group metals. [ABSTRACT FROM AUTHOR]

Background: The loss of gait automaticity is a key cause of motor deficits in Parkinson's disease (PD) patients, even at the early stage of the disease. Action observation training (AOT) shows promise in enhancing gait automaticity. However, effective assessment methods are lacking. We aimed to propose a novel gait normalcy index based on dual task cost (NIDTC) and evaluate its validity and responsiveness for early-stage PD rehabilitation. Methods: Thirty early-stage PD patients were recruited and randomly assigned to the AOT or active control (CON) group. The proposed NIDTC during straight walking and turning tasks and clinical scale scores were measured before and after 12 weeks of rehabilitation. The correlations between the NIDTCs and clinical scores were analyzed with Pearson correlation coefficient analysis to evaluate the construct validity. The rehabilitative changes were assessed using repeated-measures ANOVA, while the responsiveness of NIDTC was further compared by t tests. Results: The turning-based NIDTC was significantly correlated with multiple clinical scales. Significant group-time interactions were observed for the turning-based NIDTC (F = 4.669, p = 0.042), BBS (F = 6.050, p = 0.022) and PDQ-39 (F = 7.772, p = 0.011) tests. The turning-based NIDTC reflected different rehabilitation effects between the AOT and CON groups, with the largest effect size (p = 0.020, Cohen's d = 0.933). Conclusion: The turning-based NIDTC exhibited the highest responsiveness for identifying gait automaticity improvement by providing a comprehensive representation of motor ability during dual tasks. It has great potential as a valid measure for early-stage PD diagnosis and rehabilitation assessment. Trial registration Chinese Clinical Trial Registry: ChiCTR2300067657 [ABSTRACT FROM AUTHOR]

Excessive sugar intake poses a significant risk factor for non-communicable diseases. A positive healthy eating (PHE) intervention was developed to promote low-sugar dietary practices in families. The PHE intervention capitalized on positive psychological constructs to overcome barriers to health behavior change by helping families associate feelings of joy, gratitude, and savoring with healthy eating. In a cluster randomized controlled trial, 1983 participants from 1467 families were recruited in Hong Kong. PHE included a core and booster session. Data were collected at pre-intervention, post-intervention, and at 1-month and 3-month follow-up. Compared to the control, PHE showed greater increase in intention to change at post-intervention, engagement in low-sugar dietary practices individually and with family members at 3-month follow-up, and greater reduction in sugar-sweetened beverage intake at 1-month and 3-month follow-up. Intentions to change mediated PHE's effects on low-sugar dietary practices. Focus group interviews revealed the behavior-change process and family quality of life. [ABSTRACT FROM AUTHOR]

Background: Primary care physicians (PCPs) are doctors in primary health care institutions, namely village clinics, township health centers and community health service centers (stations) who are themain providers of primary health care services in primary health care settings. Improving the overall health status of the population requires the support of a large number of primary care physicians; however, the job satisfaction of this group has not been sufficiently emphasized and recognized. Objective: The purpose of this study was to examine the effects of primary care physicians' work-family conflict on their job satisfaction, as well as the mediating role of burnout and the moderating role of social support. Methods: This cross-sectional study was conducted fromFebruary 2023 to March 2023. Participants were 749 primary care physicians from four cities of Xuzhou, Linyi, Huaibei, and Shangqiu in the Huaihai Economic Zone of China. SPSS statistical analysis was used to evaluate the relationship between work-family conflict, social support, burnout and job satisfaction among medical workers. Results: Work-family conflict had a significant negative effect on job satisfaction (b = -0.36, p< 0.001), after adding burnout in the model, work-family conflict also negatively predicted job satisfaction (b = -0.32, p< 0.001). Social support had a moderating effect on the direct effect of burnout on job satisfaction (b = 0.00, t = 2.66, p< 0.01, 95%CI [0.001, 0.007]), the predictive effect of burnout on job satisfaction at high level of social support (b = -0.45, p< 0.001) was higher than a low level of social support (b = -0.33, p< 0.001). Conclusions: This study demonstrated the negative impact of work-family conflict on primary care physicians' job satisfaction, as well as the mediating role of burnout and the moderating role of social support on burnout and job satisfaction, which are important for improving primary care physicians' job satisfaction and enhancing the quality of primary care in the future. [ABSTRACT FROM AUTHOR]

Gulf War Illness (GWI) is a chronic multisymptom disorder that affects approximately 25–32% of Gulf War veterans and is characterized by a number of symptoms such as cognitive impairment, psychiatric disturbances, chronic fatigue and gastrointestinal distress, among others. While the exact etiology of GWI is unknown, it is believed to have been caused by toxic exposures encountered during deployment in combination with other factors such as stress. In the present study we sought to evaluate the hypothesis that exposure to the toxin permethrin could prime neuroinflammatory stress response and elicit psychiatric symptoms associated with GWI. Specifically, we developed a mouse model of GWI, to evaluate the effects of chronic permethrin exposure followed by unpredictable stress. We found that subjecting mice to 14 days of chronic permethrin exposure followed by 7 days of unpredictable stress resulted in the development of depression-like behavior. This behavioral change coincided with distinct alterations in the microglia phenotype, indicating microglial activation in the hippocampus. We revealed that blocking microglial activation through Gi inhibitory DREADD receptors in microglia effectively prevented the behavioral change associated with permethrin and stress exposure. To elucidate the transcriptional networks impacted within distinct microglia populations linked to depression-like behavior in mice exposed to both permethrin and stress, we conducted a single-cell RNA sequencing analysis using 21,566 single nuclei collected from the hippocampus of mice. For bioinformatics, UniCell Deconvolve was a pre-trained, interpretable, deep learning model used to deconvolve cell type fractions and predict cell identity across spatial datasets. Our bioinformatics analysis identified significant alterations in permethrin exposure followed by stress-associated microglia population, notably pathways related to neuronal development, neuronal communication, and neuronal morphogenesis, all of which are associated with neural synaptic plasticity. Additionally, we observed permethrin exposure followed by stress-mediated changes in signal transduction, including modulation of chemical synaptic transmission, regulation of neurotransmitter receptors, and regulation of postsynaptic neurotransmitter receptor activity, a known contributor to the pathophysiology of depression in a subset of the hippocampal pyramidal neurons in CA3 subregions. Our findings tentatively suggest that permethrin may prime microglia towards a state of inflammatory activation that can be triggered by psychological stressors, resulting in depression-like behavior and alterations of neural plasticity. These findings underscore the significance of synergistic interactions between multi-causal factors associated with GWI. [ABSTRACT FROM AUTHOR]

This mixed-methods study investigates the complex factors affecting the psychological well-being of Chinese EFL teachers, particularly the direct effects of mindfulness and growth mindset, and the mediating role of teaching enthusiasm. A quantitative survey (n = 268) was distributed to EFL teachers across diverse provinces in China. Established scales measured psychological well-being, mindfulness, growth mindset, and teaching enthusiasm. The data was analyzed using structural equation modeling (SEM) with two phases in SPSS and AMOS software. A qualitative phase, employing semi-structured interviews with a subsample (n = 12) of participants, provided deeper insights into the lived experiences of teachers. The quantitative analysis yielded positive direct relationships between mindfulness, growth mindset, and well-being. Notably, teaching enthusiasm emerged as a significant mediating factor. These findings suggest that mindfulness and growth mindset indirectly influence well-being through their positive impact on teaching enthusiasm. The qualitative data corroborated these results, showcasing how mindfulness practices and a growth mindset contribute to a more passionate and fulfilling teaching experience. These combined findings underscore the importance of promoting mindfulness, growth mindset, and teaching enthusiasm to enhance the psychological well-being of Chinese EFL teachers. [ABSTRACT FROM AUTHOR]

Background: Perceived Stress has been shown as a key contributor to sleep quality, but the underlying mechanism between perceived stress and sleep quality remains unknown. This study aimed to investigate the impact of perceived stress on sleep quality of college students and the chain mediating roles of presence of meaning in life (PML) and depression, as well as the moderating role of search for meaning in life (SML). Methods: Participants were 8178 college students (4599 boys and 3579 girls; Mage = 19.10 years, SD = 1.08) who completed self-report questionnaire, including the Perceived Stress Scale (PSS), the Pittsburgh Sleep Quality Index (PSQI), the Meaning in Life Questionnaire (MLQ), and the Patient Health Questionnaire-9 (PHQ-9). Results: The results showed that higher perceived stress was directly related to poorer sleep quality. This negative impact on sleep quality was mediated through the chained roles of PML and depression. Additionally, the study found that SML moderates the influence of perceived stress, PML and depression on sleep quality. Specifically, for individuals actively search for meaning, the adverse effects of perceived stress and depression on sleep quality are diminished. Concurrently, the positive influence of PML on sleep quality is enhanced. Conclusion: This study revealed that the PML and depression mediate the effect of perceived stress on sleep quality, with SML playing a significant protective role. These results emphasize the necessity of integrating strategies to enhance PML and SML into interventions designed to improve emotion management and sleep quality among college students. [ABSTRACT FROM AUTHOR]

CD8+ T cells are the workhorses executing adaptive anti-tumour response, and targets of various cancer immunotherapies. Latest advances have unearthed the sheer heterogeneity of CD8+ tumour infiltrating lymphocytes, and made it increasingly clear that the bulk of the endogenous and therapeutically induced tumour-suppressive momentum hinges on a particular selection of CD8+ T cells with advantageous attributes, namely the memory and stem-like exhausted subsets. A scrutiny of the contemporary perception of CD8+ T cells in cancer and the subgroups of interest along with the factors arbitrating their infiltration contextures, presented herein, may serve as the groundwork for future endeavours to probe further into the regulatory networks underlying their differentiation and migration, and optimise T cell-based immunotherapies accordingly. [ABSTRACT FROM AUTHOR]

Facilitating the restoration of upper limb functionality is a key objective of stroke rehabilitation. This study aimed to compare the effects of boxing and scapular stabilization training on scapular mobility, balance angle, muscle strength, motor function, and satisfaction in hemiparetic patients. A total of sixty patients were randomly assigned to one of three groups: the boxing group (BG; n = 20), the scapular stabilization group (SSG; n = 20), or the control group (CG; n = 20) which received no treatment at all. Each treatment group participated in three sessions per week for a total of eight weeks. The scapular mobility, balance angle (SBA), muscle strength, upper extremity motor functions (Manual Function Test-MFT), and treatment satisfaction were evaluated. The BG group showed greater improvements in the SBA (F = 59,951; p = 0.000; η2 = 0.682), mobility-frontal plane (F = 7998; p = 0.000; η2 = 0.222), mobility-sagittal plane (F = 91,632; p = 0.000; η2 = 0.766), and mobility-transverse plane (F = 48,713; p = 0.000; η2 = 0.635) than did the CG group. BG strengthened the serratus anterior (F = 42,227; p = 0.000; η2 = 0.601), while SSG strengthened the infraspinatus (F = 31,772; p = 0.000; η2 = 0.532) more than did CG. Compared with those in the SSG, supraspinatus (F = 52,589; p = 0.000; η2 = 0.653), upper trapezius (F = 42,890; p = 0.000; η2 = 0.605), anterior deltoideus (F = 30,844; p = 0.000; η2 = 0.524), latissimus dorsi (F = 84,345; p = 0.000; η2 = 0.751), MFTs (F = 52,363; p = 0.000; η2 = 0.652) and satisfaction (p = 0.008) were greater in the BG. Both approaches had a beneficial impact on the recovery process. However, boxing training was more effective than scapular stabilization training for several parameters. Clinical Trial Number: NCT05568173 date 5/10/2022. [ABSTRACT FROM AUTHOR]

Background: Passion fruit (Passiflora edulis) is loved for its delicious flavor and nutritious juice. Although studies have delved into the cultivation and enhancement of passion fruit varieties, the underlying factors contributing to the fruit's appealing aroma remain unclear. Methods: This study analyzed the full-length transcriptomes of two passion fruit cultivars with different flavor profiles: "Tainong 1" (TN1), known for its superior fruit flavor, and "Guihan 1" (GH1), noted for its strong environmental resilience but lackluster taste. Utilizing PacBio Iso-Seq and Illumina RNA-Seq technologies, we discovered terpene synthase (TPS) genes implicated in fruit ripening that may help explain the flavor disparities. Results: We generated 15,913 isoforms, with N50 lengths of 1,500 and 1,648 bp, and mean lengths of 1,319 and 1,463 bp for TN1 and GH1, respectively. Transcript and isoform lengths ranged from a maximum of 7,779 bp to a minimum of 200 and 209 bp. We identified 14,822 putative coding DNA sequences (CDSs) averaging 1,063 bp, classified 1,007 transcription factors (TFs) into 84 families. Additionally, differential expression analysis of ripening fruit from both cultivars revealed 314 upregulated and 43 downregulated unigenes in TN1 compared to GH1. The top 10 significantly enriched Gene Ontology (GO) terms for the differentially expressed genes (DEGs) indicated that TN1's upregulated genes were primarily involved in nutrient transport, whereas GH1's up-regulated genes were associated with resistance mechanisms. Meanwhile, 17 PeTPS genes were identified in P. edulis and 13 of them were TPS-b members. A comparative analysis when compared PeTPS with AtTPS highlighted an expansion of the PeTPS-b subfamily in P. edulis, suggesting a role in its fruit flavor profile. Conclusion: Our findings explain that the formation of fruit flavor is attributed to the upregulation of essential genes in synthetic pathway, in particular the expansion of TPS-b subfamily involved in terpenoid synthesis. This finding will also provide a foundational genetic basis for understanding the nuanced flavor differences in this species. [ABSTRACT FROM AUTHOR]