151. The egg-counter: a novel microfluidic platform for characterization of Caenorhabditis elegans egglaying.
- Author
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Banse, Stephen A., Jarrett, Cody M., Robinson, Kristin J., Blue, Benjamin W., Shaw, Emily L., and Phillips, Patrick C.
- Subjects
ANIMAL culture ,REPRODUCTION ,TEMPERATURE effect ,CAENORHABDITIS elegans ,MEDIA studies - Abstract
Reproduction is a fundamental process that shapes the demography of every living organism yet is often difficult to assess with high precision in animals that produce large numbers of offspring. Here, we present a novel microfluidic research platform for studying Caenorhabditis elegans' egg-laying. The platform provides higher throughput than traditional solid-media behavioral assays while providing a very high degree of temporal resolution. Additionally, the environmental control enabled by microfluidic animal husbandry allows for experimental perturbations difficult to achieve with solid-media assays. We demonstrate the platform's utility by characterizing C. elegans egg-laying behavior at two commonly used temperatures, 15 and 20 °C. As expected, we observed a delayed onset of egg-laying at 15 °C degrees, consistent with published temperature effects on development rate. Additionally, as seen in solid media studies, egg laying output was higher under the canonical 20 °C conditions. While we validated the Egg-Counter with a study of temperature effects in wild-type animals, the platform is highly adaptable to any nematode egg-laying research where throughput or environmental control needs to be maximized without sacrificing temporal resolution. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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