1. Conversion of steam-exploded cedar into ethanol using simultaneous saccharification, fermentation and detoxification process.
- Author
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Asada C, Sasaki C, Takamatsu T, and Nakamura Y
- Subjects
- Batch Cell Culture Techniques, Fermentation, Glucose metabolism, Metabolic Networks and Pathways, Renewable Energy, Saccharomyces cerevisiae metabolism, Bacillales metabolism, Cedrus metabolism, Ethanol metabolism, Wood metabolism
- Abstract
In this study, we investigated the simultaneous saccharification, fermentation and detoxification SSDF process of steam-exploded cedar using a detoxification microorganism, Ureibacillus thermosphaericus A1, to facilitate efficient ethanol production. Steam explosion was applied as a pretreatment before enzymatic saccharification followed by alcohol fermentation. The highest glucose conversion rate was observed in the sample pretreated with a steam pressure of 45atm for 5min. Alcohol production by a heat-tolerant yeast, Saccharomyces cerevisiae BA11, was inhibited strongly by inhibitory materials present in the steam-exploded cedar, such as formic acid, furfural, and 5-hydroxymethylfurfural. The maximum amount of ethanol, i.e., 0.155g ethanol/g dry steam-exploded cedar, which corresponded to 74% of the theoretical ethanol yield, was obtained using the SSDF when U. thermosphaericus A1 degraded the inhibitory materials. A fed batch SSDF culture, in which U. thermosphaericus A1 was used to maintain low concentrations of inhibitory materials, was effective for increasing the ethanol concentration., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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