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Fast and accurate detection of S. aureus and effectively kill S. aureus in the bloodstream in low doses acted as key roles in medicine and microbiology studies. In this work, sufonated-hyaluronic acid (S-HA) terminated magnetic mesoporous silica nanoparticles (MMSNs) loaded with vancomycin was successfully prepared, and S. aureus antibody was further modified on the surface of MMSNs (Ab@S-HA@MMSNs) by amidation reaction. The result showed that Ab@S-HA@MMSNs were 240 nm-sized spheres with worm-like mesoporous channels. With the aid of magnetic interaction, Ab@S-HA@MMSNs were modified on the surface of magnetic glassy carbon electrode (MGCE). This prepared immunosensor exhibited an excellent detection limit and linear range, and possessed good selectivity, stability and reproducibility. Besides, since the S-HA terminated MMSNs have anticoagulant property, the Ab@S-HA@MMSNs/MGCE showed antiadhesion property, which ensure that it can be directly applied to detect the amount of S. aureus in whole blood. What's more, with an increasing amount of S. aureus arriving at MGCE, the capping Ab@S-HA@MMSNs were degraded by Hyal which was secreted by S. aureus, and the packaged vancomycin was subsequently released. Ultimately, S. aureus can be effectively killed. Therefore, this integrated platform would be a promising multifunctional platform for achieving accurate diagnosis and efficient treatment of S. aureus bloodstream infection., (Copyright © 2019 Elsevier B.V. All rights reserved.)