1. Aurantio-Obtusin Stimulates Chemotactic Migration and Differentiation of MC3T3-E1 Osteoblast Cells.
- Author
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Vishnuprasad, Chethala N., Tomoko Tsuchiya, Shiro Kanegasaki, Joon Ho Kim, and Sung Soo Han
- Subjects
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ALKALINE phosphatase , *ALTERNATIVE medicine , *ANIMAL experimentation , *BIOLOGICAL models , *CONNECTIVE tissue cells , *DOSE-effect relationship in pharmacology , *MEDICINAL plants , *MICE , *RESEARCH funding , *STATISTICAL hypothesis testing , *T-test (Statistics) , *PLANT extracts , *DESCRIPTIVE statistics , *IN vitro studies , *PHYSIOLOGY - Abstract
Osteoporosis is one of the major metabolic bone diseases and is among the most challenging non-communicable diseases to treat. Although there is an increasing interest in identifying bioactive molecules for the prevention and management of osteoporosis, such studies principally focus only on differentiation and mineralization of osteoblasts or inhibition of osteoclast activity. Stimulation of osteoblast migration must be a promising osteoanabolic strategy for improved metabolic bone disease therapy. In this study, we show that an anthraquinone derivative, aurantio-obtusin, stimulated chemotactic migration of MC3T3-E1 osteoblast cells in a concentration-dependent manner. The use of a real-time chemotaxis analyzing system, TAXIScan, facilitated the evaluation of both velocity and directionality of osteoblast migration in response to the compound. Besides migration, the compound stimulated osteoblast differentiation andmineralization. Taken together, the data presented in this paper demonstrate that aurantio-obtusin is a promising osteoanabolic compound of natural origin with potential therapeutic applications in the prevention of osteoporosis and other metabolic bone diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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