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Your search keyword '"CHEMICAL purification"' showing total 2 results
2 results on '"CHEMICAL purification"'

1. Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. II: Dynamics of the integrated separation on ion exchange and hydrophobic interaction chromatography media.

Author

Marek, Wojciech, Muca, Renata, Woś, Sylwia, Piątkowski, Wojciech, and Antos, Dorota

Subjects
*MONOCLONAL antibodies, *HAMSTERS as laboratory animals, *IMMUNOGLOBULIN G, *CHEMICAL purification, *SEPARATION (Technology), *CHROMATOGRAPHIC analysis, *HYDROPHOBIC interactions
Abstract

Highlights: [•] Retention behavior of monoclonal IgG1 was investigated on IEC and HIC media. [•] Dynamics of both processes was dominated by kinetic effects. [•] A short-cut method was proposed for mathematical modeling. [•] A dynamic model was used for optimization of the integrated process IEC/HIC. [ABSTRACT FROM AUTHOR]

Published
2013
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2. Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. I: Assessment of different separation concepts.

Author

Marek, Wojciech, Muca, Renata, Woś, Sylwia, Piątkowski, Wojciech, and Antos, Dorota

Subjects
*MONOCLONAL antibodies, *IMMUNOGLOBULIN G, *HAMSTERS as laboratory animals, *CHEMICAL purification, *SEPARATION (Technology)
Abstract

Highlights: [•] Monoclonal anybody IgG1 was isolated out of a CHO derived supernatant. [•] Different purification techniques were used based on IEC, HIC and ATPE. [•] The best performance was achieved by integrating IEC and HIC. [•] The on-column dilution was used for feed loading in the integrated process. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
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