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Cucumber plants are highly susceptible to the hemibiotroph oomycete Phytophthora melonis. However, the mechanism of resistance to cucumber blight remains poorly understood. Here, we demonstrated that cucumber plants with impairment in the biosynthesis of brassinosteroids (BRs) or gibberellins (GAs) were more susceptible to P. melonis. By contrast, increasing levels of endogenous BRs or exogenously application of 24-epibrassinolide enhanced the resistance of cucumber plants against P. melonis. Furthermore, we found that both knockout and overexpression of the BR biosynthesis gene CYP85A1 reduced the endogenous GA 3 content compared with that of wild-type plants under the condition of inoculation with P. melonis, and the enhancement of disease resistance conferred by BR was inhibited in plants with silencing of the GA biosynthetic gene GA20ox1 or KAO. Together, these findings suggest that GA homeostasis is an essential factor mediating BRs-induced disease resistance. Moreover, BZR6, a key regulator of BR signaling, was found to physically interact with GA20ox1, thereby suppressing its transcription. Silencing of BZR6 promoted endogenous GA biosynthesis and compromised GA-mediated resistance. These findings reveal multifaceted crosstalk between BR and GA in response to pathogen infection, which can provide a new approach for genetically controlling P. melonis damage in cucumber production., (© 2024 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)

Cellulose, the major component of secondary cell walls, is the most abundant renewable long-chain polymer on earth. Nanocellulose has become a prominent nano-reinforcement agent for polymer matrices in various industries. We report the generation of transgenic hybrid poplar overexpressing the Arabidopsis gibberellin 20-oxidase1 gene driven by a xylem-specific promoter to increase gibberellin (GA) biosynthesis in wood. X-ray diffraction (XRD) and sum frequency generation spectroscopic (SFG) analyses showed that cellulose in transgenic trees was less crystalline, but the crystal size was larger. The nanocellulose fibrils prepared from transgenic wood had an increased size compared to those from wild type. When such fibrils were used as a reinforcing agent in sheet paper preparation, the mechanical strength of the paper was significantly enhanced. Engineering the GA pathway can therefore affect nanocellulose properties, providing a new strategy for expanding nanocellulose applications. [ABSTRACT FROM AUTHOR]

The key scientific problem to be solved in this paper is the optimal development and utilization model and the economic evaluation model of China's land-phase shale oil and gas resources, and the purpose of the research is to promote the large-scale commercial development and utilization of China's shale oil and gas resources, and to safeguard China's oil and gas energy security and the sustainable development of the economy. The article proposes to adopt the small surface element volume method (oil content rate method) to evaluate the pure shale oil resources, adopt the Cobb–Douglas production function model as the optimization model to measure the boundary production capacity of shale oil and gas, construct the optimal development and utilization model for shale oil and gas resources considering the five first-level safeguard indexes, namely, science and technology (A), capital (K), talents (L), reserves (S) and ecological environment (E), and establish the basic constraint model for the optimal development and utilization of shale oil and gas resources. The basic constraint model, as well as the evaluation model of economic coefficients for the development and utilization of shale oil and gas resources were established. The pure shale oil resources are mainly calculated based on the movable oil content of shale. In the paper, the S1 of normal pyrolysis (300 °C) is regarded as movable oil, and the sum of S1 and evaporated hydrocarbon (light hydrocarbon) loss is the movable oil content of shale. The integrated geological-physical exploration-engineering comprehensive evaluation of China's land-phase shale oil-rich and high-yielding "sweet spot" is an important prerequisite for the realization of shale oil and gas resources to build production scale and effective development, and the least-squares method is used to estimate the average production function, the distance to the maximum value of the residuals, and the boundary capacity production function. The average production function and residual maximum distance are estimated by the least squares method, and the production function of the boundary capacity is derived, and the quotient of the boundary capacity and the actual capacity is calculated to get the capacity utilization rate, which can be used to analyze the potential of future shale oil and gas growth. The development of shale oil and gas resources in a target block requires comprehensive consideration of the first-level guarantee indicators such as science and technology (A), capital (K), talents (L), reserves (S) and ecological environment (E), as well as more than 10 s-level indicators and a number of third-level indicators, in order to ensure that the oil companies maximize their profits by organizing the development and production. The economic coefficient can be expressed as the ratio of economically recoverable resources to geological reserves. The larger the economic coefficient for the development and utilization of shale oil and gas resources is, the better the economy of the area is, and the larger the proportion of shale oil and gas resources that can be exploited. There is little special literature on the optimal development and utilization model of shale oil and gas resources and energy security among many research results at home and abroad. The evaluation of pure shale oil using the small surface element volume method (oil content rate method) and the construction of the boundary capacity calculation model, the optimal development of the basic constraints model and the economic evaluation model that we have determined, although they can not yet fully cover all the links and factors related to the development and utilization of shale oil and gas resources, are not yet fully covered by our research work. However, our research work has given the model more geological and economic theoretical connotations, and provided an economic basis and technical reference for the large-scale and commercial development and utilization of shale oil and gas resources as an effective alternative to oil imports. [ABSTRACT FROM AUTHOR]

Subsea pipelines are vital arteries transporting oil, gas, and water over long distances and play a critical role in the global resource supply chain. However, they are the most vulnerable to damage from both human-made and natural causes and are characterized by inherent inaccessibility. As a result, routine inspection and monitoring technologies, both reliable and at the lowest possible cost, are needed to ensure their longevity. To fill this need, the use of transient-test-based techniques is proposed. In this first paper of a set of two companion papers, attention is focused on the selection of the appropriate maneuver that generates pressure waves and then on the planned steps—i.e., the sequence of actions—functional to the execution of the transient tests in the best flow conditions for effective fault detection. A brief review of the available fault detection technologies with their limitations is also offered. Finally, the performance of the proposed procedure is evaluated mainly in terms of the stability of the pressure regime prior to the execution of the transient test. [ABSTRACT FROM AUTHOR]

Multi-packer completion strings have played a pivotal role in secure exploitation of oil and gas, increasing production and mitigating greenhouse gas emissions. While the integration of multi-packer structures has proven advantageous in oil and gas exploration, it has introduced complexities in load distribution, consequently giving rise to safety concerns. This study undertakes a thorough examination of the mechanical analysis pertaining to multi-packer completion strings. We present an analytical model for predicting the axial forces acting on the multi-packer string, utilizing the geometric constraint arising from the immobility of packers. It is demonstrated that the pressure differentials at the packers exhibit uniqueness in relation to both initial and boundary conditions, as well as the geometrical constraint. This paper provides an analytical solution for these pressure differentials. Novel concepts regarding the eigen-matrix of an N-packer completion string, influenced solely by Poisson's ratio, a virtual (N + 1) th packer and the eigen-depth and eigen-ratio of two adjacent packers, are proposed and their applications are discussed. Furthermore, this paper delves into a deeper examination of the multi-packer string's underlying mechanism. A consistent algorithm grounded in geometric analysis is developed based on the analytical model. Validation of our model is performed using three practical cases across various operation conditions, and the results demonstrate the efficacy of this methodology in accurately predicting failure occurrences. Sensitivity analysis results further substantiate the robustness of this method in practical applications. Additionally, it has been shown that strategically positioning the packers in areas where the string is highly prone to fractures significantly enhances the safety of the multi-packer string system. The findings presented in this paper offer a foundational framework for analyzing the mechanical behavior of constrained strings. Furthermore, there is potential for the development of the analytical model to incorporate additional factors, such as string system with packers of semi-free or free movement. The proposed method is also of fundamental significance for safety evaluation of string systems in carbon storage projects, which is obtaining increasing attention in the context of carbon neutralization. [ABSTRACT FROM AUTHOR]

Pogostemon cablin (Blanco) Benth (Patchouli) is an aromatic herb extensively used in pharmaceutical and cosmetic industries. Sesquiterpenes are the characteristic constitutes in patchouli which are synthesized in the glandular trichomes on leaves and stems. Gibberellic acid (GA), a tetracyclic diterpenoid, plays a crucial role in the formation of glandular trichome. However, the diterpene biosynthesis remains largely unknown in patchouli. Here we identified a small diterpene synthases (diTPSs) family comprising three class II diTPSs (PatCPS1-3) and three class I diTPSs (PatKSL1 and PatGLS1-2). These diTPSs are functionally characterized using a yeast heterologous expression system. PatCPS1 was identified as an ent-copalyl diphosphate synthase (ent-CPS), in combination with PatKSL1, yield ent-kaurene, the precursor of GA, indicating their involvement in primary metabolism. PatCPS2 converted GGPP into (+)-8, 13-copalyl diphosphate (CPP). No activity was detected for PatCPS3, PatGLS1 and PatGLS2. Three ohnologs of PatCPS1 were further characterized to explore the possible functional differentiation of ent-CPS during the evolution of tetraploid hybrid patchouli genome. GC-MS analysis showed all ohnologs are functional ent-CPSs, demonstrating the functional conservation of PatCPS1 during evolution. Expression profiling by qRT-PCR showed PatCPS1 and PatKSL1 are ubiquitously expressed in all tissues, consistent with their involvement in primary metabolism. Conversely, PatCPS2 and PatCPS3 were predominantly expressed in the above ground parts, indicating a role in specialized metabolism. In summary, these findings clarify the early stages of GA biosynthesis in patchouli and provide gene elements for further metabolic engineering of sesquiterpenes via diterpenoids., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

The mechanisms by which low light accelerates starch macromolecules degradation by auxin and gibberellin (GA) in geophytes during sprouting remain largely unknown. This study investigated these mechanisms in saffron, grown under low light (50 μmol m -2  s -1 ) and optimal light (200 μmol m -2  s -1 ) during the sprouting phase. Low light reduced starch concentration in corms by 34.0 % and increased significantly sucrose levels in corms, leaves, and leaf sheaths by 19.2 %, 9.8 %, and 134.5 %, respectively. This was associated with a 33.3 % increase in GA 3 level and enhanced auxin signaling. Leaves synthesized IAA under low light, which was transported to the corms to promote GA synthesis, facilitating starch degradation through a 228.7 % increase in amylase activity. Exogenous applications of GA and IAA, as well as the use of their synthesis or transport inhibitors, confirmed the synergistic role of these phytohormones in starch metabolism. The unigenes associated with GA biosynthesis and auxin signaling were upregulated under low light, highlighting the IAA-GA module role in starch degradation. Moreover, increased respiration rate and invertase activity, crucial for ATP biosynthesis and the tricarboxylic acid cycle, were consistent with the upregulation of related unigenes, suggesting that auxin signaling accelerates starch degradation by promoting energy metabolism. Upregulated of auxin signaling (CsSAUR32) and starch metabolism (CsSnRK1) genes under low light suggests that auxin directly regulate starch degradation in saffron corms. This study elucidates that low light modulates auxin and GA interactions to accelerate starch degradation in saffron corms during sprouting, offering insights for optimizing agricultural practices under suboptimal light conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Pathogenesis-related protein 1 (PR-1) is an antimicrobial protein involved in systemic acquired resistance (SAR) in plants, but its regulatory role and interactions with other pathways remain unclear. In this study, we functionally characterize WsPR-1 gene of Withania somnifera in Nicotiana tabacum to elucidate its role in plant defense, growth, and development. Interestingly, transgenic tobacco plants with increased levels of cytokinin (CK) and decreased gibberellins (GAs) exhibited stunted shoot growth, an underdeveloped root system, modified leaf morphology, reduced seed pod production, and delayed leaf senescence. Transcriptional analysis revealed that WsPR-1 overexpression downregulated the GA 20-oxidase (GA20ox) gene involved in GA biosynthesis while upregulating GA 2-oxidase (GA2ox), a GA catabolic enzyme. Moreover, transcript levels of FRUITFULL (FUL) and LEAFY (NFL2) flowering genes exhibited a decrease in WsPR-1 plants, which could explain the delayed flowering and reduced seed pod development in transgenic plants. Confocal microscopy confirmed increased lignin deposition in stem cross-sections of WsPR-1 transgenic plants, supported by gene expression analysis and lignin content quantification. Additionally, our findings also suggest the involvement of Knotted1-like homeobox (KNOX) gene in enhancing cytokinin levels. This study highlights PR-1's regulatory role in plant growth and development, with potential to boost crop yields and enhance resilience., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Abscisic acid (ABA) and gibberellin (GA) are major regulators of seed dormancy, an adaptive trait closely associated with preharvest sprouting. This study examined transcriptional regulation of ABA and GA metabolism genes and modulation of ABA and GA levels in seeds of barley genotypes exhibiting a range of dormancy phenotype. We observed a very strong negative correlation between genetic variation in seed germination and embryonic ABA level (r = 0.85), which is regulated by transcriptional modulation of HvNCED1 and/or HvCYP707A genes. A strong positive correlation was evident between variation in seed germination and GA level (r = 0.64), mediated via transcriptional regulation of GA biosynthesis genes, HvGA20ox2 and/or HvGA3oxs, and GA catabolism genes, HvGA2ox3 and/or HvGA3ox6. Modulation of the ABA and GA levels in the genotypes led to the prevalence of ABA to GA level ratio that exhibited a very strong negative correlation (r = 0.84) with seed germination, highlighting the importance of a shift in ABA/GA ratio in determining genetic variation of dormancy in barley seeds. Our results overall show that transcriptional regulation of specific ABA and GA metabolism genes underlies genetic variation in ABA/GA ratio and seed dormancy, reflecting the potential use of these genes as molecular tools for enhancing preharvest sprouting resistance in barley., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.)

This study presents novel methodologies and materials for selectively and sensitively determining gibberellin traces in licorice to address food safety concerns. A novel hydrophilic imprinted resin-graphene oxide composite (HMIR-GO) was developed with fast mass transfer, high adsorption capacity, and exceptional aqueous recognition performance for gibberellin. Leveraging the advantages of molecular imprinting, hydrophilic resin synthesis, and rapid mass transfer characteristics of GO, HMIR-GO was employed as an adsorbent, showing resistance to matrix interference. Coupled with HPLC, a rapid and selective method for determining gibberellin was established. Under optimal conditions, the method exhibited a wide linear range (0.02-5.00 μg g -1 , r = 0.9999), low detection limits (3.3 ng g -1 ), and satisfactory recoveries (92.0-98.4%), enabling the accurate and rapid detection of gibberellin in licorice. This study introduces a pioneering strategy for the selective extraction and determination of trace gibberellin levels, offering insights for similar applications in functional foods., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Both selenium (Se) and gibberellins (GA3) can alleviate cadmium (Cd) toxicity in plants. However, the application of Se and GA3 as foliar spray to against Cd stress on soybean and its related mechanisms have been poorly explored. Herein, this experiment evaluated the effects of Se and GA3 alone and combined application on soybean rhizosphere microenvironment, Cd accumulation and growth of soybean seedlings. The results revealed that both Se and GA3 can effectively decrease the accumulation of Cd in soybean seedlings. Foliar application of Se, GA3 and their combination reduced Cd contents in soybean seedlings respectively by 21.70 %, 27.53 % and 45.07 % when compared with the control treatment, suggest a synergistic effect of Se and GA3 in decreasing Cd accumulation. Se and GA3 also significantly increased diversity and abundance of the metabolites in rhizosphere, which consequently played an important role in shaping rhizosphere bacteria community and improve rhizosphere soil physicochemical properties of Cd contaminated soil, as well as decreased the Cd available forms contents but enhance the immobilized form levels. Overall, this study affords a novel approach on mitigating Cd accumulation in soybean seedlings which is attributed to Se and GA3 regulated interplay among rhizosphere soil metabolites, bacteria community and cadmium speciation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

The F-box protein (FBP) family plays diverse functions in the plant kingdom, with the function of many members still unrevealed. In this study, a specific FBP called PmFBK2, containing Kelch repeats from Persicaria minor, was functionally investigated. Employing the yeast two-hybrid (Y2H) assay, PmFBK2 was found to interact with Skp1-like proteins from P. minor, suggesting its potential to form an E3 ubiquitin ligase, known as the SCF complex. Y2H and co-immunoprecipitation tests revealed that PmFBK2 interacts with full-length PmGID1b. The interaction marks the first documented binding between these two protein types, which have never been reported in other plants before, and they exhibited a negative effect on gibberellin (GA) signal transduction. The overexpression of PmFBK2 in the kmd3 mutant, a homolog from Arabidopsis, demonstrated the ability of PmFBK2 to restore the function of the mutated KMD3 gene. The function restoration was supported by morphophysiological and gene expression analyses, which exhibited patterns similar to the wild type (WT) compared to the kmd3 mutant. Interestingly, the overexpression of PmFBK2 or PmGID1b in Arabidopsis had opposite effects on rosette diameter, seed weight, and plant height. This study provides new insights into the complex GA signalling. It highlights the crucial roles of the interaction between FBP and the GA receptor (GID1b) in regulating GA responses. These findings have implications for developing strategies to enhance plant growth and yield by modulating GA signalling in crops., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

Despite ample knowledge of the effect of warm-dry storage on breaking dormancy in chincuya (Annona purpurea Moc. & Sessé ex Dunal) seeds, this paper investigates an important new aspect of this process—the applicability of seed treatment with gibberellic acid and its influence on seed viability and fatty acid profile. Chincuya is an underutilized, tropical wild species native to Mexico, with potential as a biopesticide. The impact of warm-dry storage and gibberellic acid treatment on seed viability and germination was determined to improve that knowledge further. Also, the content and kinetics of fatty acids in chincuya seeds were profiled during warm-dry storage. Seed treatments included storage time (0, 3, 6, 9, and 12 months), seed imbibition for 72 h in gibberellic acid at 350 mg L−1, and distilled water imbibition for 72 h for the control treatment. Seed viability was determined using the TTZ classic test. Imbibed seeds were germinated on paper towels and incubated at 30 °C during the day and 25 °C at night in a 12:12 h dark/light photoperiod. The lipid profile was obtained by gas chromatography. During the storage treatment, seeds stayed in darkness at 25 ± 3 °C. Results showed that high viability remained up to month 9 in storage when it significantly decreased. Freshly harvested seeds germinated at a statistically significantly lower rate (26.1%) than six-month-old seeds (65.6%). The profile of fatty acids breaks down as palmitic acid (33.41%), stearic acid (7.72%), and arachidic acid (1.30%), for a total of 42.44% of saturated fatty acids. The detected unsaturated fatty acids (52.15%) were palmitoleic acid (1.52%), oleic acid (43.37%), and linoleic acid (7.24%). No significant changes were detected in fatty acid content; however, the highest fatty acid content (92.84%) occurred with the highest germination rate. Regression analysis showed significant content changes: palmitoleic and palmitic acids increased while oleic acid decreased during dry storage. Arachidic acid showed a slight downward trend. Also, fatty acid content significantly differed between intact seeds (unstored and unincubated) and latent seeds (unstored, incubated, and ungerminated). The total fatty acids content for latent seeds was significantly lower. These results show that Annona purpurea seeds present morphophysiological dormancy. Also, we document the highest germination rate reported (65.6%) and report the presence of arachidic and palmitoleic acids in chincuya seeds. [ABSTRACT FROM AUTHOR]

Biodiesel production from microalgae presents an innovative solution for renewable energy. This study investigates biodiesel production using Tetradesmus obliquus ON506010.1 by optimizing substrates, selenium and gibberellic acid. Using 15 µg/L selenium, lipid content and biomass productivity reached 35.45 %±0.92 and 0.178 g/L/day ± 0.051. With 50 µM gibberellic acid, biomass productivity and lipid content peaked at 0.785 ± 0.101 g/L/day and 38.95 %±0.35, surpassing the control. Fatty acid composition, biodiesel properties, and mRNA expression of lipid synthesis enzymes (acetyl CoA carboxylase (ACC) and fatty acid desaturase (FAD)) correlated. Combining 10 µg/L selenium with 75 µM gibberellic acid with response surface methodology (RSM) increased lipid content (42.80 % ±0.11) and biomass productivity (0.964 g/L/day ± 0.128). ACC and FAD upregulation validated this enhancement, with a 4.4-fold increase in FAD expression. Fatty acid composition and most biodiesel properties met international standards demonstrating Tetradesmus obliquus ON506010.1's potential for sustainable biodiesel production with better cold flow property and oxidative stability., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The challenge of desert farming with a high salt level has become an ecological task due to salt stress negatively affecting plant growth and reproduction. The current study deals with the cultivation of sorghum under salt stress conditions to counteract the effect of chitosan and gibberellic acid (GA 3 ). Here, the effects of chitosan, GA 3 and nano-composite (GA 3 @chitosan) on biochemical contents, growth and seed yield of sorghum under salinity stress conditions were studied. The results showed that spraying with GA 3 @chitosan increased sorghum grain yield by 2.07, 1.81 and 1.64 fold higher than salinity stressed plants, chitosan treatment and GA 3 treatment, respectively. Additionally, compared to the control of the same variety, the GA 3 @chitosan spraying treatment improved the concentration of microelements in the grains of the Shandweel-1 and Dorado by 24.51% and 18.39%, respectively for each variety. Furthermore, spraying GA 3 @chitosan on sorghum varieties increased the accumulation of the macroelements N, P, and K by 34.03%, 47.61%, and 8.67% higher than salt-stressed plants, respectively. On the other hand, the proline and glycinebetaine content in sorghum leaves sprayed with nano-composite were drop by 51.04% and 11.98% less than stressed plants, respectively. The results showed that, in Ras Sudr, the Shandweel-1 variety produced more grain per feddan than the Dorado variety. These findings suggest that GA 3 @chitosan improves the chemical and biochemical components leading to a decrease in the negative effect of salt stress on the plant which reflects in the high-yield production of cultivated sorghum plants in salt conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

A monoacylglycerol, α-(7Z,10Z,13Z)-hexadeca-7,10,13-trienoic acid monoglyceride, extracted from leaves of Japanese radish (Raphanus sativus) has been reported as an anti-bolting compound (ABC); however, the mechanism how ABC inhibits the plant bolting has been remained to be elucidated. In this paper, it was found that exogenous applications of ABC led Arabidopsis thaliana seedlings decrease transcriptional levels of AtKO and increase those of AtGA2ox to reduce endogenous levels of gibberellins (GAs) to retard A. thaliana growth, whose physiological phenomena were counteracted by exogenous applications of ent-kaurenoic acid and GA3, respectively. Furthermore, α-oleanolic acid monoglyceride having both activities of supressing KO and enhancing GA2ox inductions retarded A. thaliana growth post germination, although α-palmitic acid monoglyceride having only the effect of supressing KO induction was not enough to suppress the growth. These experimental data supported the idea that dual function was essential for ABC to show biological activity. [ABSTRACT FROM AUTHOR]

The precise mechanism behind the association between plants' reactions to cadmium (Cd) stress and brassinosteroid (BR) remains unclear. In the current investigation, Cd stress quickly increased the endogenous BR concentration in the rice roots. Exogenous BR also increased the hemicellulose level in the root cell wall, which in turn increased its capacity to bind Cd. Simultaneously, the transcription level of genes responsible for root Cd absorption was decreased, including Natural Resistance-Associated Macrophage Protein 1/5 (OsNRAMP1/5) and a major facilitator superfamily gene called OsCd1. Ultimately, the increased expression of Heavy Metal ATPase 3 (OsHMA3) and the decreased expression of OsHMA2, which was in charge of separating Cd into vacuoles and translocating Cd to the shoots, respectively, led to a decrease in the amount of Cd that accumulated in the rice shoots. In contrast, transgenic rice lines overexpressing OsGSK2 (a negative regulator in BR signaling) accumulated more Cd, while OsGSK2 RNA interference (RNAi) rice line accumulated less Cd. Furthermore, BR increased endogenous Gibberellic acid (GA) level, and applying GA could replicate its alleviative effect. Taken together, BR decreased Cd accumulation in rice by mediating the cell wall's fixation capacity to Cd, which might relied on the buildup of the GA., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Photoperiod is a crucial environmental cue for phenological responses, including growth cessation and winter dormancy in perennial woody plants. Two regulatory modules within the photoperiod pathway explain bud dormancy induction in poplar (Populus spp.): the circadian oscillator LATE ELONGATED HYPOCOTYL 2 (LHY2) and GIGANTEA-like genes (GIs) both regulate the key target for winter dormancy induction FLOWERING LOCUS T2 (FT2). However, modification of LHY2 and GIs cannot completely prevent growth cessation and bud set under short-day (SD) conditions, indicating that additional regulatory modules are likely involved. We identified PtoHY5a, an orthologs of the photomorphogenesis regulatory factor ELONGATED HYPOCOTYL 5 (HY5) in poplar (Populus tomentosa), that directly activates PtoFT2 expression and represses the circadian oscillation of LHY2, indirectly activating PtoFT2 expression. Thus, PtoHY5a suppresses SD-induced growth cessation and bud set. Accordingly, PtoHY5a knockout facilitates dormancy induction. PtoHY5a also inhibits bud-break in poplar by controlling gibberellic acid (GA) levels in apical buds. Additionally, PtoHY5a regulates the photoperiodic control of seasonal growth downstream of phytochrome PHYB2. Thus, PtoHY5a modulates seasonal growth in poplar by regulating the PtoPHYB2-PtoHY5a-PtoFT2 module to determine the onset of winter dormancy, and by fine-tuning GA levels to control bud-break., Competing Interests: Conflict of interest statement. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

The estrogenic effect of plant growth regulators has been received little attention, which leads to the lack of relevant toxicity data. In this study, the estrogenic effect induced by gibberellin with ERα-dependent manner was found by E-screen and western blot methods, and the electrochemical signals of MCF-7 cells regulated by gibberellin and fulvestrant were investigated. The results showed that the electrochemical signals of MCF-7 cells were increased by gibberellin, while reduced by fulvestrant significantly, and displayed an extremely sensitive response to the effects of estrogenic effect induced by ERα agonist and antagonist. Western blot results showed that the expressions of phosphoribosyl pyrophosphate amidotransferase and hypoxanthine nucleotide dehydrogenase in de novo purine synthesis and adenine deaminase in catabolism were more effective regulated by gibberellin and fulvestrant, resulting in significant changes of the levels of guanine, hypoxanthine and xanthine in cells, and then electrochemical signals. The results provide a theoretical basis for the establishment of new electrochemical detection method of the estrogenic effect of plant regulators., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Surface reconstruction plays a pivotal role in various fields, including reverse engineering, and oil and gas exploration. However, errors in available data and insufficient surface morphology information often introduce uncertainty into the reconstruction. It is crucial to accurately characterize and visualize the uncertainty in surface reconstruction for risk analysis and planning further data collection. To this end, this paper proposes an uncertainty characterization method based on twin support vector regression. First, various modeling data are effectively integrated and the information contained in the high-confidence sample is efficiently utilized through the uncertainty interval generated by quantiles and upper/lower bound constraints. Second, well-path points are incorporated by imposing inequality constraints on the corresponding prediction points. Finally, in order to reduce computation time, the problem of uncertainty characterization is formulated as two smaller-scale quadratic programming. The results obtained from a real fault dataset and a synthetic dataset validate the effectiveness of the proposed method. When well data are available, the generated uncertainty envelopes are constrained by well data, which can partially mitigate reconstruction uncertainties. [ABSTRACT FROM AUTHOR]

Gibberellic acid (GA3), produced industrially by Fusarium fujikuroi, stands as a crucial plant growth regulator extensively employed in the agriculture filed while limited understanding of the global metabolic network hinders researchers from conducting rapid targeted modifications. In this study, a small-molecule compounds-based targeting technology was developed to increase GA3 production. Firstly, various small molecules were used to target key nodes of different pathways and the result displayed that supplement of terbinafine improved significantly GA3 accumulation, which reached to 1.08 g/L. Subsequently, lipid and squalene biosynthesis pathway were identified as the key pathways influencing GA3 biosynthesis by transcriptomic analysis. Thus, the strategies including in vivo metabolic engineering modification and in vitro supplementation of lipid substrates were adopted, both contributed to an enhanced GA3 yield. Finally, the engineered strain demonstrated the ability to achieve a GA3 yield of 3.24 g/L in 5 L bioreactor when utilizing WCO as carbon source and feed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Gibberellic acid (GA3), one of the most plant growth stimulator, is widely applied in agricultural regions and in beer industry. However, GA3 residue remained in soil and water can cause toxicity to all organisms. In this study, we investigated the mechanisms of GA3-induced hepatic injury in gibel carp (Carassius auratus gibelio). We found that GA3 exposure caused oxidative stress, endoplasmic reticulum stress (ERS), and apoptosis. The gibel carp exposed to GA3 exhibited significant alteration in erythrocyte nuclei. GA3 induced liver damage, as indicated by increasing the aminopherase activities. GA3 led to oxidative stress by increasing malondialdehyde content and decreasing the activities of CAT and GPx. GA3 stimulated ERS and increased the expression of grp78, perk, eif2s1α, chop, atf4, ire1α, xbp1, and atf6. Additionally, GA3 down-regulated the level of anti-apoptotic gene Bcl-2 and up-regulated the levels of pro-apoptotic genes bax and caspase-3. Overall results demonstrated that GA3 caused hepatic injury in gibel carp by increasing oxidative stress, ERS, and apoptosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Gibberellins (GAs) play a crucial role in regulating secondary growth in angiosperms, but their effects on the secondary growth of gymnosperms are rarely reported. In this study, we administered exogenous GA 3 to two-year-old P. massoniana seedlings, and examined its effects on anatomical structure, physiological and biochemical changes, and gene expression in stems. The results showed that exogenous GA 3 could enhance xylem development in P. massoniana by promoting cell division. The content of endogenous hormone (including auxins, brassinosteroids, and gibberellins) were changed and the genes related to phytohormone biosynthesis and signaling pathway, such as GID1, DELLA, TIR1, ARF, SAUR, CPD, BR6ox1, and CYCD3, were differentially expressed under GA 3 treatment. Furthermore, GA 3 and BR (brassinosteroid) might act synergistically in promoting secondary growth in P. massoniana. Additionally, lignin content was significantly increased after GA 3 treatment accompanied by the express of lignin biosynthesis related genes. PmCAD (TRINITY_DN142116_c0_g1), a crucial gene involved in the lignin biosynthesis, was cloned and overexpressed in Nicotiana benthamiana, significantly promoting the xylem development and enhancing stem lignification. It was regarded as a key candidate gene for improving stem growth of P. massoniana. The findings of this study have demonstrated the impact of GA 3 treatment on secondary growth of stems in P. massoniana, providing a foundation for understanding the molecular regulatory mechanism of stem secondary growth in Pinaceae seedlings and offering theoretical guidance for cultivating new germplasm with enhanced growth and yield., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Masson SAS. All rights reserved.)

This study aimed to examine the effects of gibberellic acid (GBA) on growth, hemato-biochemical parameters related to liver functions, digestive enzymes, and immunological response in Oreochromis niloticus. Besides, the probable underlying mechanisms were explored by assessing antioxidant, apoptotic, and immune-related gene expression. Furthermore, the likelihood of restoration following alpha-lipoic acid (LIP) dietary supplementation was explored. The fish (average initial weight 30.75 ± 0.46) were equally classified into four groups: the control group, the LIP group (fed on a basal diet plus 600 mg/kg of LIP), the GBA group (exposed to 150 mg GBA/L), and the GBA + LIP group (exposed to 150 mg GBA/L and fed a diet containing LIP and GBA) for 60 days. The study findings showed that LIP supplementation significantly reduced GBA's harmful effects on survival rate, growth, feed intake, digestive enzymes, and antioxidant balance. Moreover, the GBA exposure significantly increased liver enzymes, stress markers, cholesterol, and triglyceride levels, all of which were effectively mitigated by the supplementation of LIP. Additionally, LIP addition to fish diets significantly minimized the histopathological alterations in the livers of GBA-treated fish, including fatty change, sharply clear cytoplasm with nuclear displacement to the cell periphery, single-cell necrosis, vascular congestion, and intralobular hemorrhages. The GBA-induced reduction in lysozyme activity, complement C3, and nitric oxide levels, together with the downregulation of antioxidant genes (cat and sod), was significantly restored by dietary LIP. Meanwhile, adding LIP to the GBA-exposed fish diets significantly corrected the aberrant expression of hsp70, caspase- 3, P53, pcna, tnf-a, and il-1β in O. niloticus liver. Conclusively, dietary LIP supplementation could mitigate the harmful effects of GBA exposure on fish growth and performance, physiological conditions, innate immunity, antioxidant capability, inflammatory response, and cell apoptosis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Oil-immersed power transformers generate characteristic gases after failure,Therefore, it is necessary to analyse and monitor the soluble gases in transformer oil samples. In this study, the DFT calculation method was used to study the adsorption properties of H2, CO, C2H2, and C2H4 gases in oil on both intrinsic MoTe2 and Rh-doped MoTe2 films. In order to analyse the adsorption characteristics, this paper first obtains the most stable Rh-doped MoTe2 monolayer model through the modelling and computational analysis of different doping sites, then, the adsorption of these gases on the material surface is studied by analysing adsorption energy, charge transfer, total state density, parting density, energy band structure, differential charge density map, molecular front orbital and desorption time, and finally concludes that Rh-MoTe2 monolayer film is the ideal material for hydrogen sensing elements, due to the extremely long desorption time of CO gas, indicating that CO gas is hard to desorption on the surface of adsorbent,which shows that this process has played a certain role in promoting CO removal. Highlights Study focused on gas adsorption using DFT: Rh-MoTe2 ideal for hydrogen sensing and effective for CO removal, intrinsic MoTe2 ineffective and required metal doping. Rh doping enhanced conductivity and gas adsorption capacity in MoTe2. Rh-MoTe2 was suitable for detecting H2, C2H2, and C2H4 gases. Desorption times vary with temperature, with CO removal particularly effective at higher temperatures. Findings suggest potential applications for Rh-MoTe2 in gas sensing and removal. [ABSTRACT FROM AUTHOR]

Offshore oil and gas resources play a crucial role in supplementing the energy needs of human society. The crisscrossing subsea pipeline network, which serves as vital infrastructure for the storage and transportation of offshore oil and gas, requires regular inspection and maintenance to ensure safe operation and prevent ecological pollution. In-line inspection (ILI) techniques have been widely used in the detection and inspection of potential hazards within the pipeline network. This paper offers an overview of ILI techniques used in subsea pipelines, examining their advantages, limitations, applicable scenarios, and performance. It aims to provide valuable insights for the selection of ILI technologies in engineering and may be beneficial for those involved in pipeline integrity management and planning. [ABSTRACT FROM AUTHOR]