49 results on '"Yu, Lu"'
Search Results
2. ITGB4 Serves as an Identification and Prognosis Marker Associated with Immune Infiltration in Small Cell Lung Carcinoma
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Li, Guo-Sheng, Huang, Zhi-Guang, He, Rong-Quan, Zhang, Wei, Tang, Yu-Xing, Liu, Zhi-Su, Gan, Xiang-Yu, Tang, Deng, Li, Dong-Ming, Tang, Yu-Lu, Zhan, Yan-Ting, Dang, Yi-Wu, Zhou, Hua-Fu, Zheng, Jin-Hua, Jin, Mei-Hua, Tian, Jia, and Chen, Gang
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- 2023
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3. CDK6 is a novel predictive and prognosis biomarker correlated with immune infiltrates in multiple human neoplasms, including small cell lung carcinoma
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Li, Guo-Sheng, Huang, Zhi-Guang, Li, Dong-Ming, Tang, Yu-Lu, Zheng, Jin-Hua, Yang, Lin, Feng, Yue, Peng, Jun-Xi, Li, Jing-Xiao, Tang, Yu-Xing, Zeng, Neng-Yong, Jin, Mei-Hua, Tian, Jia, Liu, Jun, Zhou, Hua-Fu, Chen, Gang, and Chen, Feng
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- 2023
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4. MMP12 serves as an immune cell–related marker of disease status and prognosis in lung squamous cell carcinoma
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Wei Zhang, Guo-Sheng Li, Xiang-Yu Gan, Zhi-Guang Huang, Rong-Quan He, Hong Huang, Dong-Ming Li, Yu-Lu Tang, Deng Tang, Wen Zou, Jun Liu, Yi-Wu Dang, Gang Chen, Hua-Fu Zhou, Jin-Liang Kong, and Hui-ping Lu
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Lung squamous cell carcinoma ,MMP12 ,Gene expression ,Prognosis ,Clinical value ,Immunity ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background Worldwide, lung squamous cell carcinoma (LUSC) has wreaked havoc on humanity. Matrix metallopeptidase 12 (MMP12) plays an essential role in a variety of cancers. This study aimed to reveal the expression, clinical significance, and potential molecular mechanisms of MMP12 in LUSC. Methods There were 2,738 messenger RNA (mRNA) samples from several multicenter databases used to detect MMP12 expression in LUSC, and 125 tissue samples were validated by immunohistochemistry (IHC) experiments. Receiver operator characteristic (ROC) curves, Kaplan–Meier curves, and univariate and multivariate Cox regression analyses were used to assess the clinical value of MMP12 in LUSC. The potential molecular mechanisms of MMP12 were explored by gene enrichment analysis and immune correlation analysis. Furthermore, single-cell sequencing was used to determine the distribution of MMP12 in multiple tumor microenvironment cells. Results MMP12 was significantly overexpressed at the mRNA level (p
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- 2023
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5. Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of Toxicodendron succedaneum
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Dongxiao Ma, Qin Zhang, Jintao Zhou, Yu Lu, Xiaomeng Duan, Chengzhong He, and Jinde Yu
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gene expression ,reference gene ,RT-qPCR ,Toxicodendron succedaneum ,Genetics ,QH426-470 - Abstract
Toxicodendron succedaneum (L.) Kuntze (T. succedaneum) is an economic tree species that produces urushiol and urushi wax, and it is of great value in industry and medicine. However, the stability of reference genes (RGs) has not been systematically reported in T. succedaneum to date. In this study, the expression of 10 candidate RGs was analyzed by RT-qPCR in different tissues (roots, stems, leaves), stress treatments (high/low temperature, drought), and hormone stimulation (jasmonic acid, JA). Then, the stability ranking of 10 candidate genes was evaluated by ∆Ct analysis and three software programs: geNorm, NormFinder, and BestKeeper. Finally, RefFinder was used to comprehensively analyze the expression stability of 10 candidate genes. The comprehensive analysis showed that TsRG05/06, TsRG01/06, and TsRG03/ACT were stable under high/low-temperature stress, drought stress, and JA treatment, respectively. TsRG03 and ACT had stable expression in different tissues. While the TsRG03 and ACT were recommended as the suitable RGs for T. succedaneum in all samples. Meanwhile, UBQ was the least suitable as a reference gene for T. succedaneum. In addition, the results of geNorm showed that the combination of two stable RGs could make the results of gene expression more accurate. These results provide alternative RGs for the study of gene function, correction, and normalization of target gene expression and directed molecular breeding in T. succedaneum.
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- 2022
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6. Dietary Agaricus blazei Spent Substrate Improves Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae In Vivo
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Po-Tsang Lee, Yu-Sheng Wu, Chung-Chih Tseng, Jia-Yu Lu, and Meng-Chou Lee
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immunoregulator ,Agaricus blazei ,spent substrate ,Streptococcus agalactiae ,gene expression ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.
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- 2022
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7. Study on the correlation between CD80/CD163 and clinical prognosis and the syndrome differentiation in patients with colorectal cancer.
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Jun-Yu Ke, Fan-Chang Wu, Zhen-Fan He, Zheng-Lin Liu, Jian-Feng Luo, Jin-Bin Song, Long Li, Zhuo-Jian Huang, Tian-Yu Lu, Qi-Sheng Zhong, Yan-Hai Lyu, Qun Du, Yong-Qiang Wu, Xin-Lin Chen, and Yan-Wu Li
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COLORECTAL cancer ,CANCER patients ,CHINESE medicine ,GENE expression ,CARCINOEMBRYONIC antigen ,HEREDITARY nonpolyposis colorectal cancer - Abstract
Background: To analyze the expression of cluster of differentiation 80 (CD80)/cluster of differentiation 163 (CD163) in colorectal cancer (CRC) patients and their correlation with the syndrome of traditional Chinese medicine, pathology and prognosis. Methods: (1) The correlation between the pathological characteristics of 232 postoperative CRC patients and the deficiency and excess syndromes of traditional Chinese medicine was analyzed using the chi-square test, Spearman’s correlation, and Cox regression methods. (2) Immunohistochemistry and quantitative real-time PCR were used to detect the expression of CD80 and CD163 in cancer and para cancer tissues of CRC patients. (3) The relationships between the changes of CD80 and CD163 and the prognosis of CRC patients with deficiency syndrome. Patient survival were analyzed using cardinality and Cox regression proportional-hazards model regression. Results: (1) The degree of differentiation and tumor node metastasis stage of CRC were statistically different between patients with deficiency and excess syndromes (P < 0.05); carcinoembryonic antigen and carbohydrate antigen19-9were highly expressed in the excess syndrome group, and both were significantly correlated with the distribution of traditional Chinese medicine syndromes (P <0.001); deficiency and excess syndromes, and carbohydrate antigen 19-9 were all independent factors affecting the postoperative survival of CRC patients. (2) The distribution of post-operative survival in CRC patients was significantly correlated with the distribution of the disease type (P<0.001). (3) The expression levels of CD163 protein and message RNA were significantly higher in CRC cancer tissues than in paraneoplastic tissues (P <0.001); whereas the expression of CD80 was significantly higher in paraneoplastic tissues than in cancer tissues(P < 0.001). (4) The expression levels of CD80/CD163 were significantly different indifferent parts of the tissues of patients with deficiency and excess syndromes (P <0.001). (5) CRC patients with high CD80 expression and low CD163 expression had longer survival cycles (P < 0.001). Conclusion: The malignant progression of CRC patients with deficiency syndrome is faster than that with excess syndrome. The correlation between deficiency and excess syndromes and the expression levels of CD80 and CD163 could be an independent risk factor for the survival prognosis of patients with CRC. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Propofol suppresses OGD/R-induced ferroptosis in neurons by inhibiting the HIF-1α/YTHDF1/BECN1 axis.
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Ma, Hongyan, Ye, Dongxue, Liu, Yuqing, Wu, Pei, Yu, Lu, Guo, Libo, Gao, Yang, Liu, Ying, Yan, Haiyan, and Shi, Jinghui
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PROPOFOL ,IN vitro studies ,GLUTATHIONE ,NEURONS ,WESTERN immunoblotting ,APOPTOSIS ,PRECIPITIN tests ,GENE expression ,CELL survival ,MALONDIALDEHYDE ,NEUROPROTECTIVE agents ,MESSENGER RNA ,RESEARCH funding ,TRANSCRIPTION factors ,CELL lines ,REPERFUSION injury ,PHARMACODYNAMICS - Abstract
Ischemia/reperfusion (I/R) is a pathological process that causes severe damage. Propofol is known to alleviate I/R-related injury; however, the exact function and underlying mechanisms are not fully understood. Using an oxygen glucose deprivation/re-oxygenation (OGD/R) method, an in vitro I/R injury model was induced. The cell viability and the level of Fe
2+ , glutathione synthetase (GSH), and malondialdehyde (MDA) were evaluated using kits. Luciferase reporter gene assay, chromatin immunoprecipitation, and RNA immunoprecipitation (RIP) were used to verify the interaction between molecules. The m6A level of BECN1 mRNA was determined through methylated RIP. Propofol-treated OGD/R models showed reduced levels of Fe2+ and MDA, while the cell viability and the level of GSH increased. Propofol inhibited ferroptosis by down-regulating HIF-1α in OGD/R-treated HT22 cells. HIF-1α is bound to the promoter region of YTHDF1 to promote its transcription, and YTHDF1 promoted ferroptosis by stabilizing the mRNA of BECN1. The suppressive effect of propofol on OGD/R-induced ferroptosis was reversed by the overexpression of YTHDF1. Our study revealed that the HIF-1α/YTHDF1/BECN1 axis in OGD/R-treated HT22 cells promotes ferroptosis, and administration of propofol can inhibit this axis to avoid cell death. This study provides a novel insight for the neuroprotective function of propofol. [ABSTRACT FROM AUTHOR]- Published
- 2023
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9. Identification and analysis of key hypoxia- and immune-related genes in hypertrophic cardiomyopathy.
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Yu, Haozhen, Gu, Lanxin, Du, Linfang, Dong, Zhao, Li, Zhuang, Yu, Mujun, Yin, Yue, Wang, Yishi, Yu, Lu, and Ma, Heng
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HYPERTROPHIC cardiomyopathy ,SUDDEN death ,IMPLANTABLE cardioverter-defibrillators ,GENE expression ,GENES ,GENE regulatory networks ,SUPPORT vector machines - Abstract
Background: Hypertrophic cardiomyopathy (HCM), an autosomal dominant genetic disease, is the main cause of sudden death in adolescents and athletes globally. Hypoxia and immune factors have been revealed to be related to the pathology of HCM. There is growing evidence of a role for hypoxia and inflammation as triggers and enhancers in the pathology in HCM. However, the role of hypoxia- and immune-related genes in HCM have not been reported. Methods: Firstly, we obtained four HCM-related datasets from the Gene Expression Omnibus (GEO) database for differential expression analysis. Immune cells significantly expressed in normal samples and HCM were then screened by a microenvironmental cell population counter (MCP-counter) algorithm. Next, hypoxia- and immune-related genes were screened by the LASSO + support vector machine recursive feature elimination (SVM-RFE) and weighted gene co-expression network analysis (WGCNA). Single-gene enrichment analysis and expression validation of key genes were then performed. Finally, we constructed a competing endogenous RNA (ceRNA) network of key genes. Results: In this study, 35 differentially expressed hypoxia genes were found. By using LASSO + SVM-RFE analysis, 10 more targets with differentially expressed hypoxia genes were identified. The MCP-count algorithm yielded five differentially expressed immune cells, and after assessing them for WGCNA characteristics, 612 immune genes were discovered. When hypoxia and immune genes were combined for cross-tabulation analysis, three hypoxia- and immune-related genes (ATP2A2, DDAH1, and OMA1) were identified. Conclusion: Based on hypoxia characteristic genes, three key genes were identified. These were also significantly related to immune activation, which proves a theoretical basis and reference value for studying the relationship between HCM and hypoxia and immunity. [ABSTRACT FROM AUTHOR]
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- 2023
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10. MAN5, a Glycosyl Hydrolase Superfamily Protein, Is a Key Factor Involved in Cyanide-Promoted Seed Germination in Arabidopsis thaliana.
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Yu, Lu-Lu and Xu, Fei
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GERMINATION , *GENETIC overexpression , *GENE expression , *GENETIC mutation , *SEED treatment , *ARABIDOPSIS thaliana - Abstract
Seed germination is the complex adaptive trait of higher plants influenced by a large number of genes and environmental factors. Numerous studies have been performed to better understand how germination is controlled by various environmental factors and applied chemicals, such as cyanide. However, still very little is known about the molecular mechanisms of how extrinsic signals regulate seed germination. Our and previous studies found that non-lethal cyanide treatment promotes seed germination, but the regulatory mechanism is unclear. In this study, we found that a low concentration of cyanide pretreatment significantly enhanced the expression of endo-β-mannanase 5 (MAN5) gene in Arabidopsis thaliana, and the mutation of this gene impaired cyanide-mediated seed germination. In contrast, overexpression of MAN5 gene enhanced Arabidopsis seed germination ability under both normal and salt stress conditions. Further studies showed that the expression of the MAN5 gene was negatively regulated by ABA insensitive 5 (ABI5); In abi5 mutant seeds, the expression of the MAN5 gene was increased and the seed germination rate was accelerated. Additionally, cyanide pretreatment markedly reduced the gene expression of ABI5 in Arabidopsis seeds. Taken together, our data support the involvement of MAN5 as a key gene in cyanide-mediated seed germination and confirm the role of ABI5 as a critical negative factor involved in cyanide-regulated MAN5 gene expression. [ABSTRACT FROM AUTHOR]
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- 2023
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11. Carnosine-Based Reversal of Diabetes-Associated Cognitive Decline via Activation of the Akt/mTOR Pathway and Modulation of Autophagy in a Rat Model of Type 2 Diabetes Mellitus.
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Ndolo, Rodgers Odhiambo, Yu, Lu, Zhao, Yan, Lu, Jinying, Wang, Gao, Zhao, Xinmin, Ren, Yi, and Yang, Jing
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COGNITION disorders , *BIOMARKERS , *IN vivo studies , *NEUROPEPTIDES , *AUTOPHAGY , *ANIMAL experimentation , *SIGNAL peptides , *AMINOGLYCOSIDES , *BLOOD sugar , *COGNITION , *TYPE 2 diabetes , *RATS , *CELLULAR signal transduction , *GENE expression , *NEUROPROTECTIVE agents , *RESEARCH funding , *COGNITIVE testing , *DIETARY fats , *DISEASE complications - Abstract
Introduction: Carnosine can suppress secondary complications in diabetes and show robust neuroprotective activity against neurodegenerative diseases. Here, we report that carnosine ameliorates diabetes-associated cognitive decline in vivo through the modulation of autophagy. Methods: A high-fat diet (HFD) and one intraperitoneal injection of 30 mg/kg streptozotocin (STZ) were used to induce type 2 diabetes mellitus in Sprague-Dawley rats. The rats were randomly divided into five groups: control (CON), HFD/STZ, and three intragastric carnosine treatment groups receiving low (100 mg/kg), medium (300 mg/kg), and high (900 mg/kg) doses over 12 weeks. Body weight, blood glucose levels, and cognitive function were continuously monitored. From excised rat hippocampi, we determined superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels; carnosine concentration; protein expressions of Akt, mTOR and the autophagy markers LC3B and P62 and performed histopathological evaluations of the cornu ammonis 1 region. Results: The HFD/STZ group showed increased blood glucose levels and decreased body weight compared to the CON group. However, there were no significant differences in body weight and blood glucose levels between carnosine-treated and -untreated HFD-STZ-induced diabetic rats. Diabetic animals showed obvious learning and memory impairments in the Morris water maze test compared to the CON group. Compared to those in the HFD/STZ group, carnosine increased SOD activity and decreased MDA levels, increased hippocampal carnosine concentration, increased p-Akt and p-mTOR expression, decreased LC3B and P62 expression, alleviated neuronal injuries, and improved cognitive performance in a dose-dependent manner. Conclusion: Independent of any hyperglycemic effect, carnosine may improve mild cognitive impairments by mitigating oxidative stress, activating the Akt/mTOR pathway, and modulating autophagy in the hippocampus of type 2 diabetic rats. [ABSTRACT FROM AUTHOR]
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- 2023
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12. Heterologous Expression and Bioactivity Determination of Monochamus alternatus Antibacterial Peptide Gene in Komagataella phaffii (Pichia pastoris).
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Chu, Xu, Jiang, Di, Yu, Lu, Li, Ming, Wu, Songqing, Zhang, Feiping, and Hu, Xia
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GENE expression ,BACILLUS thuringiensis ,PEPTIDES ,PICHIA pastoris ,BIOLOGICAL control of insects ,PINEWOOD nematode ,ANTIMICROBIAL peptides - Abstract
Insects have evolved to form a variety of complex natural compounds to prevent pathogen infection in the process of a long-term attack and defense game with various pathogens in nature. Antimicrobial Peptides (AMPs) are important effector molecules of the insect immune response to the pathogen invasion involved in bacteria, fungi, viruses and nematodes. The discovery and creation of new nematicides from these natural compounds is a key path to pest control. A total of 11 AMPs from Monochamus alternatus were classified into 3 categories, including Attacin, Cecropin and Defensin. Four AMP genes were successfully expressed by Komagataella phaffii KM71. The bioassay results showed that the exogenous expressed AMPs represented antimicrobial activity against Serratia (G
− ), Bacillus thuringiensis (G+ ) and Beauveria bassiana and high nematicide activity against Bursaphelenchus xylophilus. All four purified AMPs' protein against B. xylophilus reached LC50 at 3 h (LC50 = 0.19 mg·mL−1 of MaltAtt-1, LC50 = 0.20 mg·mL−1 of MaltAtt-2 and MaltCec-2, LC50 = 0.25 mg·mL−1 of MaltDef-1). Furthermore, the AMPs could cause significant reduction of the thrashing frequency and egg hatching rate, and the deformation or fracture of the body wall of B. xylophilus. Therefore, this study is a foundation for further study of insect biological control and provides a theoretical basis for the research and development of new insecticidal pesticides. [ABSTRACT FROM AUTHOR]- Published
- 2023
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13. Temperature and metal ions regulate larval diapause termination via the 20‐hydroxyecdysone and juvenile hormone pathways in Monochamus alternatus.
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Yang, Meijiao, Li, Guoqiang, Yu, Lu, Du, Shijie, Jiang, Di, Chu, Xu, Wang, Kai, Wu, Songqing, Wang, Rong, Zhang, Feiping, and Hu, Xia
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DIAPAUSE ,JUVENILE hormones ,METAL ions ,ION temperature ,ENZYME-linked immunosorbent assay ,GENE expression - Abstract
BACKGROUND: Diapause allows insects to survive harsh environments, and its termination is crucial for their normal development after diapause. However, little is known about the regulatory pathways and signals involved in insect diapause termination. RESULTS: We discovered that high temperature (25 °C) influenced larval diapause termination in Monochamus alternatus. Likewise, metal ions (Ca2+) promoted diapause termination by reducing diapause duration. We combined transcriptomic and metabolomic analyses to investigate changes in gene expression and metabolism in diapause‐terminated larvae treated with high temperature (MaHt) and metal ions (MaCa). Hormone biosynthesis and metabolism contained the highest proportion of significant differentially expressed genes (DEGs) in the two groups. 20‐hydroxyecdysone (20E) and juvenile hormone (JH) were closely related to diapause termination in M. alternatus. RNA interference (RNAi) experiments verified that 20E biosynthesis (CYP314a1) and degradation (CYP18a1), JH biosynthesis (FOHSDR‐1) and degradation (JHEH) genes affected the larval diapause duration significantly. In addition, dysfunction of CYP314a1 resulted in increased larval mortality (P < 0.01), reduced pupation rate and emergence rate (P < 0.05). Enzyme‐linked immunosorbent assay (ELISA) analysis showed that the ecdysone content decreased after dsCYP314a1 injection and JH content increased after dsJHEH injection. CONCLUSION: The results indicate that genes CYP314a1, CYP18a1, FOHSDR‐1 and JHEH mediated 20E and JH biosynthesis and degradation to regulate diapause termination in M. alternatus. We elucidated the molecular mechanism underlying the regulation of diapause termination and provided a basis for the prevention and control of M. alternatus infestation. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
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- 2023
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14. Downregulation of zinc finger protein 71 in laryngeal squamous cell carcinoma tissues and its potential molecular mechanism and clinical significance: a study based on immunohistochemistry staining and data mining.
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Jiang, Fang-Cheng, Luo, Jia-Yuan, Dang, Yi-Wu, Lu, Hui-Ping, Li, Dong-Ming, Huang, Zhi-Guang, Tang, Yu-Lu, Fang, Ye-Ying, Tang, Yu-Xing, Su, Ya-Si, Dai, Wen-Bin, Pan, Shang-Ling, Feng, Zhen-Bo, Chen, Gang, and He, Juan
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ZINC-finger proteins ,SQUAMOUS cell carcinoma ,GENE expression ,DATA mining ,TIGHT junctions - Abstract
Background: The molecular mechanism of laryngeal squamous cell carcinoma (LSCC) is not completely clear, which leads to poor prognosis and treatment difficulties for LSCC patients. To date, no study has reported the exact expression level of zinc finger protein 71 (ZNF71) and its molecular mechanism in LSCC. Methods: In-house immunohistochemistry (IHC) staining (33 LSCC samples and 29 non-LSCC samples) was utilized in analyzing the protein expression level of ZNF71 in LSCC. Gene chips and high-throughput sequencing data collected from multiple public resources (313 LSCC samples and 192 non-LSCC samples) were utilized in analyzing the exact mRNA expression level of ZNF71 in LSCC. Single-cell RNA sequencing (scRNA-seq) data was used to explore the expression status of ZNF71 in different LSCC subpopulations. Enrichment analysis of ZNF71, its positively and differentially co-expressed genes (PDCEGs), and its downstream target genes was employed to detect the potential molecular mechanism of ZNF71 in LSCC. Moreover, we conducted correlation analysis between ZNF71 expression and immune infiltration. Results: ZNF71 was downregulated at the protein level (area under the curve [AUC] = 0.93, p < 0.0001) and the mRNA level (AUC = 0.71, p = 0.023) in LSCC tissues. Patients with nodal metastasis had lower protein expression level of ZNF71 than patients without nodal metastasis (p < 0.05), and male LSCC patients had lower mRNA expression level of ZNF71 than female LSCC patients (p < 0.01). ZNF71 was absent in different LSCC subpopulations, including cancer cells, plasma cells, and tumor-infiltrated immune cells, based on scRNA-seq analysis. Enrichment analysis showed that ZNF71 and its PDCEGs may influence the progression of LSCC by regulating downstream target genes of ZNF71. These downstream target genes of ZNF71 were mainly enriched in tight junctions. Moreover, downregulation of ZNF71 may influence the development and even therapy of LSCC by reducing immune infiltration. Conclusion: Downregulation of ZNF71 may promote the progression of LSCC by reducing tight junctions and immune infiltration; this requires further study. [ABSTRACT FROM AUTHOR]
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- 2022
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15. The Arabidopsis J-Protein AtDjC5 Facilitates Thermotolerance Likely by Aiding in the ER Stress Response.
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Shen, Ting-Ting, Wang, Lin, Shang, Chun-Huan, Zhen, Yi-Cai, Fang, Yu-Lu, Wei, Li-Li, Zhou, Ting, Bai, Jiao-Teng, and Li, Bing
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ARABIDOPSIS ,GENE expression ,ENDOPLASMIC reticulum ,ARABIDOPSIS thaliana ,SURVIVAL rate - Abstract
AtDjC5 belongs to the J-protein family in Arabidopsis thaliana. Its biological functions remain unclear. In this study, we examined the roles of AtDjC5 in resisting heat stress using reverse genetic analysis. After the seedlings were exposed directly to 44 °C for 90 min, AtDjC5 knockout seedlings displayed decreases in the survival rate, membrane system stability, and cell vitality compared to WT seedlings, indicating that AtDjC5 is involved in plant basal thermotolerance. The AtDjC5 knockout seedlings pre-exposed to 37 °C for 30 min exhibited decreases in the survival rate and total chlorophyll contents and increased cell death when they were subsequently exposed to 45 °C compared to the WT seedlings, indicating that AtDjC5 plays an important role in plant acquired thermotolerance. AtDjC5 was found to localize to the endoplasmic reticulum. The expression of the AtDjC5 gene was induced by heat and TM (an ER stress inducer) treatment. Furthermore, we found that the knockout of AtDjC5 inhibited ER stress-induced autophagy and the expression of ER stress-related genes. Taken together, these results suggest that AtDjC5 facilitates thermotolerance, likely by aiding in the ER stress response. [ABSTRACT FROM AUTHOR]
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- 2022
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16. Allicin-induced global gene expression profile of Saccharomyces cerevisiae
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Yu, Lu, Guo, Na, Meng, Rizeng, Liu, Bin, Tang, Xudong, Jin, Jing, Cui, Yumei, and Deng, Xuming
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- 2010
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17. Comparison between gene expression of conidia and germinating phase in Trichophyton rubrum
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Yang Li, Wang LingLing, Peng JunPing, Yu Lu, Liu Tao, Leng WenChuan, Yang Jian, Chen LiHong, Zhang WenLiang, Zhang Qian, Qi YiPeng, and Jin Qi
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- 2007
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18. DNA Methylation and SNPs in VCX are Correlated with Sex Differences in the Response to Chronic Hepatitis B
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Yuanjia Hu, Yu Zhao, Yi-Yang Hu, Qi-Long Chen, Xue-Qing Hu, Jian Chen, Yi-Yu Lu, Shi-Bing Su, and Yuan Zhou
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0301 basic medicine ,Adult ,Male ,030106 microbiology ,Immunology ,Gene Expression ,Single-nucleotide polymorphism ,Locus (genetics) ,Biology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Young Adult ,Hepatitis B, Chronic ,Sex Factors ,Virology ,Gene expression ,Genotype ,Humans ,Promoter Regions, Genetic ,Nuclear Proteins ,Methylation ,DNA Methylation ,Middle Aged ,Molecular biology ,Up-Regulation ,genomic DNA ,030104 developmental biology ,CpG site ,DNA methylation ,Molecular Medicine ,Female ,Research Article - Abstract
The study was conducted to explore the mechanisms of sex differences in the response to chronic hepatitis B (CHB) in terms of DNA methylation, SNP genotype, and gene expression. Genomic DNA was isolated from peripheral blood mononuclear cells (PBMCs) of CHB patients and healthy controls and evaluated using the Human Methylation 450 K Assay. The DNA methylation level at hg37 chromosome (CHR) X: 7810800 was further validated using pyrosequencing. SNP genotypes, VCX mRNA expression of PBMCs, and plasma VCX protein concentration were further examined using SNaPshot, RT-qPCR, and Western blot, respectively. Results showed that a total of 5529 CpG loci were differentially methylated between male and female CHB patients. DNA methylation level and CC + CT frequency at CHR X: 7810800, VCX mRNA expression of PBMCs, and plasma VCX protein concentration were higher in female than in male CHB patients. The CHR X: 7810800 locus was hypermethylated in CHB patients with CC + CT genotypes in comparison with those with the TT genotype. In cases of CC + CT genotypes, VCX mRNA expression was negatively correlated with the DNA methylation level. CHB patients with higher levels of HBV DNA, AST, and GGT or higher GPRI scores exhibited lower VCX expression. In conclusion, SNPs and DNA methylation at the CHR X: 7810800 locus cooperatively regulate VCX expression in CHB. The upregulated VCX expression in female CHB patients might represent a mechanism of protection from more severe liver dysfunction and extensive fibrosis, as observed in male CHB patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12250-019-00117-0) contains supplementary material, which is available to authorized users.
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- 2019
19. Nuclear corepressor SMRT is a strong regulator of body weight independently of its ability to regulate thyroid hormone action
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Hiroaki Shimizu, Anthony N. Hollenberg, Inna Astapova, Anthony Rosenzweig, Molly R. Gallop, Izuki Amano, Megan J. Ritter, Ronald N. Cohen, Kristen R. Vella, Federico Damilano, and Yu Lu
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Male ,0301 basic medicine ,Physiology ,Gene Expression ,Thyrotropin ,Weight Gain ,Biochemistry ,Mice ,Endocrinology ,0302 clinical medicine ,Cell Signaling ,Retinoic Acid Signaling Cascade ,Medicine and Health Sciences ,Nuclear receptor co-repressor 1 ,Mice, Knockout ,2. Zero hunger ,Multidisciplinary ,Liver Diseases ,Lipids ,Signaling Cascades ,Cell biology ,Cholesterol ,Physiological Parameters ,Liver ,Echocardiography ,030220 oncology & carcinogenesis ,Medicine ,Histone deacetylase activity ,Research Article ,Signal Transduction ,Thyroid Hormones ,Science ,Blotting, Western ,Gastroenterology and Hepatology ,Biology ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Mediator ,Downregulation and upregulation ,Genetics ,Animals ,Nuclear Receptor Co-Repressor 2 ,Obesity ,Retinoid Signaling ,Triglycerides ,Thyroid hormone receptor ,Endocrine Physiology ,Body Weight ,Biology and Life Sciences ,Cell Biology ,Glucose Tolerance Test ,Fatty Liver ,Mice, Inbred C57BL ,Thyroxine ,Retinoic acid receptor ,030104 developmental biology ,Nuclear receptor ,Insulin Resistance ,Energy Metabolism ,Corepressor - Abstract
Silencing Mediator of Retinoid and Thyroid Hormone Receptors (SMRT) and the nuclear receptor co-repressor1 (NCoR1) are paralogs and regulate nuclear receptor (NR) function through the recruitment of a multiprotein complex that includes histone deacetylase activity. Previous genetic strategies which deleted SMRT in a specific tissue or which altered the interaction between SMRT and NRs have suggested that it may regulate adiposity and insulin sensitivity. However, the full role of SMRT in adult mice has been difficult to establish because its complete deletion during embryogenesis is lethal. To elucidate the specific roles of SMRT in mouse target tissues especially in the context of thyroid hormone (TH) signaling, we used a tamoxifen-inducible post-natal disruption strategy. We found that global SMRT deletion causes dramatic obesity even though mice were fed a standard chow diet and exhibited normal food intake. This weight gain was associated with a decrease in energy expenditure. Interestingly, the deletion of SMRT had no effect on TH action in any tissue but did regulate retinoic acid receptor (RAR) function in the liver. We also demonstrate that the deletion of SMRT leads to profound hepatic steatosis in the setting of obesity. This is unlike NCoR1 deletion, which results in hepatic steatosis due to the upregulation of lipogenic gene expression. Taken together, our data demonstrate that SMRT plays a unique and CoR specific role in the regulation of body weight and has no role in TH action. This raises the possibility that additional role of CoRs besides NCoR1 and SMRT may exist to regulate TH action.
- Published
- 2019
20. Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector
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Bihua Deng, Yiwei Wang, Xi Bao, Jibo Hou, Hai Xu, Yu Lu, and Yue Xu
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0301 basic medicine ,T7 phage ,viruses ,Genetic Vectors ,Gene Expression ,Marker gene ,lcsh:Infectious and parasitic diseases ,law.invention ,DNA vaccination ,Targeting delivery ,Bacteriophage ,03 medical and health sciences ,chemistry.chemical_compound ,law ,Genes, Reporter ,Virology ,Bacteriophage T7 ,Vaccines, DNA ,Humans ,lcsh:RC109-216 ,Vector (molecular biology) ,Insertion ,Amino Acid Sequence ,biology ,Research ,Gene Transfer Techniques ,biology.organism_classification ,030104 developmental biology ,Infectious Diseases ,chemistry ,Eukaryotic expression ,Recombinant DNA ,tat Gene Products, Human Immunodeficiency Virus ,Genetic Engineering ,Peptides ,Vaccine ,DNA - Abstract
Background DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although, various phages have been used to facilitate DNA transfer by surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat protein (Tat peptide), no similar study has been conducted using T7 phage. Methods In this study, we engineeredT7 phage as a DNA targeting delivery vector to facilitate cellular internalization. We constructed recombinant T7 phages that displayed Tat peptide on their surface and carried eukaryotic expression box (EEB) as a part of their genomes (T7-EEB-Tat). Results We demonstrated that T7 phage harboring foreign gene insertion had packaged into infective progeny phage particles. Moreover, when mammalian cells that were briefly exposed to T7-EEB-Tat, expressed a significant higher level of the marker gene with the control cells infected with the wide type phage without displaying Tat peptides. Conclusion These data suggested that the potential of T7 phage as an effective delivery vector for DNA vaccine transfer.
- Published
- 2018
21. Genome-wide transcriptome analysis of the salt stress tolerance mechanism in Rosa chinensis
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Tian Xiaoming, Ci Huacong, Gui-xia Jia, Zhang Qing, Yu Lu, Wang Pengshan, and Wang Zhenyu
- Subjects
0106 biological sciences ,0301 basic medicine ,Leaves ,Molecular biology ,Gene Expression ,lcsh:Medicine ,Plant Science ,Sodium Chloride ,01 natural sciences ,Biochemistry ,Transcriptome ,Sequencing techniques ,Plant Growth Regulators ,Malondialdehyde ,Plant Resistance to Abiotic Stress ,Gene expression ,Photosynthesis ,Plant Hormones ,lcsh:Science ,Multidisciplinary ,biology ,Ecology ,Plant Biochemistry ,Plant Anatomy ,RNA sequencing ,Genomics ,Salt Tolerance ,Glutathione ,Plant Physiology ,Plant hormone ,Signal Transduction ,Research Article ,Rosa ,Biological pathway ,03 medical and health sciences ,Plant-Environment Interactions ,Genetics ,Rosa chinensis ,Plant Defenses ,Gene ,Illumina dye sequencing ,Superoxide Dismutase ,Gene Expression Profiling ,Plant Ecology ,Ecology and Environmental Sciences ,lcsh:R ,Biology and Life Sciences ,Molecular Sequence Annotation ,Cell Biology ,Plant Pathology ,biology.organism_classification ,Hormones ,Gene expression profiling ,Plant Leaves ,Research and analysis methods ,030104 developmental biology ,Gene Ontology ,Molecular biology techniques ,lcsh:Q ,Peptides ,010606 plant biology & botany - Abstract
Plants regulate responses to salt stress using biological pathways, such as signal perception and transduction, photosynthesis, and energy metabolism. Little is known about the genetics of salt tolerance in Rosa chinensis. Tineke and Hiogi are salt-tolerant and salt-sensitive varieties of R. chinensis, respectively, and are good choices for studying salt-tolerance genes. We studied leaf and root tissues from 1-year-old Hiogi and Tineke plants simultaneously grown under the same conditions. A 0.4%-mmol/L salt ion mixture was added to the basic growth medium. Illumina sequencing was used to identify differentially expressed transcripts. GO and KEGG pathway enrichment analyses were performed to identify differentially expressed genes. We identified many differentially expressed genes associated with salt tolerance. The abscisic acid-dependent signaling pathway was the main pathway that mediated the salt stress response in R. chinensis. Two pathways (plant hormone signal transduction and glutathione metabolism) were also active in salt stress responses in R. chinensis. The difference in salt tolerance in the cultivars was due to different gene sensitivity to salt in these two pathways. Roots also play a role in salt stress response. The effects of salt stress in the roots are eventually manifested in the leaves, causing changes in processes such as photosynthesis, which eventually result in leaf wilting. In Tineke, Snrk2, ABF, HSP, GSTs, and GSH1 showed high activity during salt stress, indicating that these genes are markers of salt tolerance.
- Published
- 2018
22. The proteomic response of the reef coral Pocillopora acuta to experimentally elevated temperatures
- Author
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Anderson B. Mayfield, Chi-Yu Lu, Chii-Shiarng Chen, and Yi-Jyun Chen
- Subjects
Proteomics ,0301 basic medicine ,Proteomes ,Coral ,Marine and Aquatic Sciences ,Gene Expression ,lcsh:Medicine ,Biochemistry ,Mass Spectrometry ,Transcriptome ,Symbiodinium ,Electrophoresis, Gel, Two-Dimensional ,lcsh:Science ,Cellular Stress Responses ,Multidisciplinary ,geography.geographical_feature_category ,Endosymbiosis ,biology ,Coral Reefs ,Ecology ,Messenger RNA ,Temperature ,Genomics ,Coral reef ,Nucleic acids ,Cell Processes ,Corals ,Proteome ,Transcriptome Analysis ,Research Article ,Zoology ,Marine Biology ,Acclimatization ,03 medical and health sciences ,Genetics ,14. Life underwater ,Reef ,geography ,lcsh:R ,Biology and Life Sciences ,Computational Biology ,Proteins ,Cell Biology ,Genome Analysis ,biology.organism_classification ,030104 developmental biology ,Earth Sciences ,Reefs ,RNA ,lcsh:Q - Abstract
Although most reef-building corals live near the upper threshold of their thermotolerance, some scleractinians are resilient to temperature increases. For instance, Pocillopora acuta specimens from an upwelling habitat in Southern Taiwan survived a 9-month experimental exposure to 30°C, a temperature hypothesized to induce stress. To gain a greater understanding of the molecular pathways underlying such high-temperature acclimation, the protein profiles of experimental controls incubated at 27°C were compared to those of conspecific P. acuta specimens exposed to 30°C for two, four, or eight weeks, and differentially expressed proteins (DEPs) were removed from the protein gels and sequenced with mass spectrometry. Sixty unique DEPs were uncovered across both eukaryotic compartments of the P. acuta-dinoflagellate (genus Symbiodinium) mutualism, and Symbiodinium were more likely to up-regulate protein expression in response to high temperature exposure than the coral hosts in which they resided at the 2-week sampling time. Furthermore, different cellular pathways were affected by elevated temperature exposure in each compartment; Symbiodinium tended to up-regulate the expression of proteins involved in the cellular stress response, whereas the differentially expressed host coral proteome featured numerous proteins involved in cytoskeletal structure, immunity, and metabolism. These proteome-scale data suggest that the coral host and its intracellular dinoflagellates have differing cellular strategies for acclimating to elevated temperatures.
- Published
- 2018
23. Bioinformatic identification of key pathways, hub genes, and microbiota for therapeutic intervention in Helicobacter pylori infection.
- Author
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Chen, Zhenhui, Chen, Huijuan, Yu, Lu, Xin, Hongjie, Kong, Jingjing, Bai, Yang, Zeng, Weisen, Zhang, Jumei, Wu, Qingping, and Fan, Hongying
- Subjects
HELICOBACTER pylori infections ,HELICOBACTER pylori ,GENE regulatory networks ,GENES ,GENE expression ,DATA libraries - Abstract
The pathogenic mechanisms of Helicobacter pylori infection remain to be defined, and potential interventional microbiota are just beginning to be identified. In this study, gene‐set enrichment analysis (GSEA) was used to integrate three H. pylori infection microarray data sets from the gene expression omnibus database and identified ten hallmark gene sets and 35 Kyoto encyclopedia of genes and genomes (KEGG) pathways that differed between healthy and Helicobacter pylori‐infected individuals. Weighted gene co‐expression network analysis (WGCNA) performed on two of the data sets identified three key gene coexpression modules. These modules contained 54 enriched KEGG pathways, 25 of which overlapped with the GSEA analysis, suggesting potentially important roles in H. pylori‐infection. We selected 116 hub genes from the three key modules for in vitro validation at the transcriptional level using H. pylori Sydney Strain 1 and verified the upregulation of 80. WGCNA of the microbiomes based on 20 mucosal samples and a sequence read archive data set revealed four microbiota modules correlated with H. pylori infection. The negatively correlated modules contained 11 microbiome families. These findings provide new insight into the pathogenesis of H. pylori infection and systematically identify 25 key pathways, 80 upregulated hub genes, and 11 families of candidate interventional microbiota for further research. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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24. Modulation the alternative splicing of GLA (IVS4+919G>A) in Fabry disease
- Author
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Chi Yu Lu, Dau Ming Niu, Ta Chih Liu, Shyr Yi Lin, Jan-Gowth Chang, and Wen Hsin Chang
- Subjects
0301 basic medicine ,RNA splicing ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Biochemistry ,Polymerase Chain Reaction ,Histones ,Amiloride ,Exon ,Database and Informatics Methods ,Transversion ,lcsh:Science ,Multidisciplinary ,biology ,Chemistry ,Chromosome Biology ,Chromatin Modification ,Histone Modification ,Genomics ,Exons ,Chromatin ,Cell biology ,Precipitation Techniques ,Nucleic acids ,Histone ,Epigenetics ,lipids (amino acids, peptides, and proteins) ,Sequence Analysis ,Research Article ,Bioinformatics ,Research and Analysis Methods ,Genome Complexity ,03 medical and health sciences ,PSIP1 ,Sequence Motif Analysis ,DNA-binding proteins ,Genetics ,Immunoprecipitation ,Humans ,RNA, Messenger ,Molecular Biology Techniques ,Molecular Biology ,Alpha-galactosidase ,030102 biochemistry & molecular biology ,Biology and life sciences ,Alternative splicing ,lcsh:R ,Intron ,Computational Biology ,Proteins ,nutritional and metabolic diseases ,Cell Biology ,Co-Immunoprecipitation ,Introns ,Alternative Splicing ,030104 developmental biology ,RNA processing ,alpha-Galactosidase ,biology.protein ,RNA ,Fabry Disease ,lcsh:Q ,Gene expression - Abstract
While a base substitution in intron 4 of GLA (IVS4+919G>A) that causes aberrant alternative splicing resulting in Fabry disease has been reported, its molecular mechanism remains unclear. Here we reported that upon IVS4+919G>A transversion, H3K36me3 was enriched across the alternatively spliced region. PSIP1, an adapter of H3K36me3, together with Hsp70 and NONO were recruited and formed a complex with SF2/ASF and SRp20, which further promoted GLA splicing. Amiloride, a splicing regulator in cancer cells, could reverse aberrant histone modification patterns and disrupt the association of splicing complex with GLA. It could also reverse aberrant GLA splicing in a PP1-dependant manner. Our findings revealed the alternative splicing mechanism of GLA (IVS4+919G>A), and a potential treatment for this specific genetic type of Fabry disease by amiloride in the future.
- Published
- 2017
25. Ubiquitin Ligase ATL31 Functions in Leaf Senescence in Response to the Balance Between Atmospheric CO2 and Nitrogen Availability in Arabidopsis
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Yoshie Morita, Thais Huarancca Reyes, Junji Yamaguchi, Shoki Aoyama, Takeo Sato, Yu Lu, and Lorenzo Guglielminetti
- Subjects
Senescence ,Chlorophyll ,C/N balance ,Time Factors ,Physiology ,Nitrogen ,Ubiquitin-Protein Ligases ,Mutant ,Arabidopsis ,Down-Regulation ,Plant Science ,Anthocyanins ,chemistry.chemical_compound ,Gene Knockout Techniques ,Ubiquitin ,Gene Expression Regulation, Plant ,Genes, Reporter ,Gene expression ,Biomass ,Special Focus Issue – Regular Papers ,Photosynthesis ,Chlorosis ,biology ,Arabidopsis Proteins ,fungi ,food and beverages ,Starch ,Cell Biology ,General Medicine ,Carbon Dioxide ,biology.organism_classification ,Plants, Genetically Modified ,Ubiquitin ligase ,Up-Regulation ,DNA-Binding Proteins ,Plant Leaves ,Phenotype ,chemistry ,Biochemistry ,Mutation ,biology.protein ,CO2 - Abstract
Carbon (C) and nitrogen (N) are essential elements for metabolism, and their availability, called the C/N balance, must be tightly coordinated for optimal growth in plants. Previously, we have identified the ubiquitin ligase CNI1/ATL31 as a novel C/N regulator by screening plants grown on C/N stress medium containing excess sugar and limited N. To elucidate further the effect of C/N balance on plant growth and to determine the physiological function of ATL31, we performed C/N response analysis using an atmospheric CO2 manipulation system. Under conditions of elevated CO2 and sufficient N, plant biomass and total sugar and starch dramatically increased. In contrast, elevated CO2 with limited N did not increase plant biomass but promoted leaf chlorosis, with anthocyanin accumulation and increased senescence-associated gene expression. Similar results were obtained with plants grown in medium containing excess sugar and limited N, suggesting that disruption of the C/N balance affects senescence progression. In ATL31-overexpressing plants, promotion of senescence under disrupted CO2/N conditions was repressed, whereas in the loss-of-function mutant it was enhanced. The ATL31 gene was transcriptionally up-regulated under N deficiency and in senescent leaves, and ATL31 expression was highly correlated with WRKY53 expression, a key regulator of senescence. Furthermore, transient protoplast analysis implicated the direct activation of ATL31 expression by WRKY53, which was in accordance with the results of WRKY53 overexpression experiments. Together, these results demonstrate the importance of C/N balance in leaf senescence and the involvement of ubiquitin ligase ATL31 in the process of senescence in Arabidopsis.
- Published
- 2014
26. LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network.
- Author
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Xue, Lingzi, Sun, Mintao, Wu, Zhen, Yu, Lu, Yu, Qinghui, Tang, Yaping, and Jiang, Fangling
- Subjects
GENE expression ,FRUIT ,AUXIN ,TOMATOES ,GENE regulatory networks ,TOMATO diseases & pests ,PLANT hormones ,ABIOTIC stress - Abstract
Background: Fruit cracking occurs easily under unsuitable environmental conditions and is one of the main types of damage that occurs in fruit production. It is widely accepted that plants have developed defence mechanisms and regulatory networks that respond to abiotic stress, which involves perceiving, integrating and responding to stress signals by modulating the expression of related genes. Fruit cracking is also a physiological disease caused by abiotic stress. It has been reported that a single or several genes may regulate fruit cracking. However, almost none of these reports have involved cracking regulatory networks. Results: Here, RNA expression in 0 h, 8 h and 30 h saturated irrigation-treated fruits from two contrasting tomato genotypes, 'LA1698' (cracking-resistant, CR) and 'LA2683' (cracking-susceptible, CS), was analysed by mRNA and lncRNA sequencing. The GO pathways of the differentially expressed mRNAs were mainly enriched in the 'hormone metabolic process', 'cell wall organization', 'oxidoreductase activity' and 'catalytic activity' categories. According to the gene expression analysis, significantly differentially expressed genes included Solyc02g080530.3 (Peroxide, POD), Solyc01g008710.3 (Mannan endo-1,4-beta-mannosidase, MAN), Solyc08g077910.3 (Expanded, EXP), Solyc09g075330.3 (Pectinesterase, PE), Solyc07g055990.3 (Xyloglucan endotransglucosylase-hydrolase 7, XTH7), Solyc12g011030.2 (Xyloglucan endotransglucosylase-hydrolase 9, XTH9), Solyc10g080210.2 (Polygalacturonase-2, PG2), Solyc08g081010.2 (Gamma-glutamylcysteine synthetase, gamma-GCS), Solyc09g008720.2 (Ethylene receptor, ER), Solyc11g042560.2 (Ethylene-responsive transcription factor 4, ERF4) etc. In addition, the lncRNAs (XLOC_16662 and XLOC_033910, etc) regulated the expression of their neighbouring genes, and genes related to tomato cracking were selected to construct a lncRNA-mRNA network influencing tomato cracking. Conclusions: This study provides insight into the responsive network for water-induced cracking in tomato fruit. Specifically, lncRNAs regulate the hormone-redox-cell wall network, including plant hormone (auxin, ethylene) and ROS (H
2 O2 ) signal transduction and many cell wall-related mRNAs (EXP, PG, XTH), as well as some lncRNAs (XLOC_16662 and XLOC_033910, etc.). [ABSTRACT FROM AUTHOR]- Published
- 2020
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27. Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation.
- Author
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Xu, Shangen, Yu, Lu, Teng, Qiaoyang, Li, Xuesong, Jin, Zheng, Qu, Yang, Li, Jiawei, Zhang, Qihong, Li, Zejun, and Zhao, Kai
- Subjects
- *
AVIAN influenza , *GENE expression , *DNA vaccines , *IMMUNE response , *NANOPARTICLES , *T cells - Abstract
DNA vaccination has great potential to treat or prevent avian influenza pandemics, but the technique may be limited by low immunogenicity and gene delivery in clinical testing. Here, to improve the immune efficacy of DNA vaccines against avian influenza, we prepared and tested the immunogenicity of 4 recombinant DNA vaccines containing 2 or 3 AIV antigens. The results revealed that chickens and mice immunized with plasmid DNA containing 3 antigens (HA gene from H9N2, and NA and HA genes from H5N1) exhibited a robust immune response than chickens and mice immunized with plasmid DNAs containing 2 antigenic genes. Subsequently, this study used pβH9N1SH5 as a model antigen to study the effect of dendritic polylysine (DGL) nanoparticles as a gene delivery system and adjuvant on antigen-specific immunity in mice models. At a ratio of 1:3 DGL/pβH9N1SH5 (w/w), the pβH9N1SH5/DGL NPs showed excellent physical and chemical properties, induced higher levels of HI antibodies, and larger CD3+/CD4+ T lymphocyte and CD3+/CD8+ T lymphocyte population, as well as the production of cytokines, namely, interferon (IFN)-γ, interleukin (IL)-2 compared with the naked pβH9N1SH5. Therefore, multiantigen gene expression methods using DGL as a delivery system may have broad application prospects in gene therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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28. Inhibition on Proteasome β1 Subunit Might Contribute to the Anti-Cancer Effects of Fangchinoline in Human Prostate Cancer Cells
- Author
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Yu Lu, Miao Liu, Peng Sun, Xiao-Bo Qu, Lihong Hu, Baohong Jiang, Wanying Wu, Dong Li, Li-Xing Feng, Dean Guo, Min Yang, and Xuan Liu
- Subjects
Male ,Proteasome Endopeptidase Complex ,Gene Expression ,lcsh:Medicine ,Apoptosis ,Benzylisoquinolines ,chemistry.chemical_compound ,Mice ,Bcl-2-associated X protein ,Ubiquitin ,Catalytic Domain ,Cell Line, Tumor ,LNCaP ,Animals ,Humans ,lcsh:Science ,Cell Proliferation ,bcl-2-Associated X Protein ,Multidisciplinary ,biology ,Cell growth ,lcsh:R ,Ubiquitination ,Prostatic Neoplasms ,Transfection ,Cell Cycle Checkpoints ,Antineoplastic Agents, Phytogenic ,Xenograft Model Antitumor Assays ,Recombinant Proteins ,Cell biology ,Tetrandrine ,Enzyme Activation ,Disease Models, Animal ,Protein Subunits ,Proteasome ,chemistry ,Gene Knockdown Techniques ,Cancer cell ,biology.protein ,I-kappa B Proteins ,lcsh:Q ,Proteasome Inhibitors ,Cyclin-Dependent Kinase Inhibitor p27 ,Protein Binding ,Research Article - Abstract
Fangchinoline is a bisbenzylisoquinoline alkaloid isolated from Radix Stephaniae tetrandrae S. Moore. Fangchinoline and its structure analogue, tetrandrine, exhibited direct binding affinity with recombinant human proteasome β1 subunit and also inhibited its activity in vitro. In cultured prostate PC-3 cells and LnCap cells, fangchinoline could dose-dependently inhibit cell proliferation and caspase-like activity of cellular proteasome which was mediated by proteasome β1 subunit. The inhibitive effect of fangchinoline on caspase-like activity of proteasome was also observed in purified human erythrocyte 20S proteasome. In PC-3 cells, fangchinoline induced cell cycle arrest at G0/G1 phase and apoptosis. Treatment of PC-3 tumor-bearing nude mice with fangchinoline inhibited tumor growth, induced apoptosis and also caused decrease in proteasome activities in tumor xenografts. Dose-dependent and time-dependent accumulation of ubiquitinated proteins and important proteasome substrates such as p27, Bax and IκB-α were observed in fangchinoline-treated cells. Over-expression of proteasome β1 subunit by plasmid transfection increased sensitivity of cells to the cytotoxicity of fangchinoline while knockdown of proteasome β1 subunit ameliorated cytotoxicity of fangchinoline in PC-3 cells. Results of the present study suggested that proteasome inhibition was involved in the anti-cancer effects of fangchinoline. Fangchinoline and its structure analogues might be new natural proteasome inhibitors targeting β1 subunit.
- Published
- 2015
29. Gene expression profile of peripheral blood in colorectal cancer
- Author
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Chen-En Jian, Kang-Hua Chen, Yu-Tien Chang, Hsiu-Ling Chou, Thomas Wetter, Harn-Jing Terng, Chi-Shuan Huang, Chien-Ting Chen, Chiao-Huang Lin, Chi-Wen Chang, Yun-Wen Shih, Yi-Syuan Wu, Chi-Ming Chu, Chung-Tay Yao, Chian-Yu Lu, Yu-Ching Chou, Ching-Huang Lai, Ke-Shin Lin, and Sui-Lung Su
- Subjects
Male ,Microarray ,Colorectal cancer ,Adenocarcinoma ,Retrospective Study ,Predictive Value of Tests ,Gene expression ,Biomarkers, Tumor ,Odds Ratio ,Medicine ,Humans ,Genetic Predisposition to Disease ,Aged ,Oligonucleotide Array Sequence Analysis ,Retrospective Studies ,Chi-Square Distribution ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,fungi ,Gastroenterology ,food and beverages ,Reproducibility of Results ,General Medicine ,Odds ratio ,medicine.disease ,Phenotype ,Peripheral blood ,digestive system diseases ,Gene expression profiling ,Logistic Models ,Multivariate Analysis ,Cancer research ,Female ,business ,Colorectal Neoplasms - Abstract
Optimal molecular markers for detecting colorectal cancer (CRC) in a blood-based assay were evaluated.A matched (by variables of age and sex) case-control design (111 CRC and 227 non-cancer samples) was applied. Total RNAs isolated from the 338 blood samples were reverse-transcribed, and the relative transcript levels of candidate genes were analyzed. The training set was made of 162 random samples of the total 338 samples. A logistic regression analysis was performed, and odds ratios for each gene were determined between CRC and non-cancer. The samples (n = 176) in the testing set were used to validate the logistic model, and an inferred performance (generality) was verified. By pooling 12 public microarray datasets(GSE 4107, 4183, 8671, 9348, 10961, 13067, 13294, 13471, 14333, 15960, 17538, and 18105), which included 519 cases of adenocarcinoma and 88 controls of normal mucosa, we were able to verify the selected genes from logistic models and estimate their external generality.The logistic regression analysis resulted in the selection of five significant genes (P0.05; MDM2, DUSP6, CPEB4, MMD, and EIF2S3), with odds ratios of 2.978, 6.029, 3.776, 0.538 and 0.138, respectively. The five-gene model performed stably for the discrimination of CRC cases from controls in the training set, with accuracies ranging from 73.9% to 87.0%, a sensitivity of 95% and a specificity of 95%. In addition, a good performance in the test set was obtained using the discrimination model, providing 83.5% accuracy, 66.0% sensitivity, 92.0% specificity, a positive predictive value of 89.2% and a negative predictive value of 73.0%. Multivariate logistic regressions analyzed 12 pooled public microarray data sets as an external validation. Models that provided similar expected and observed event rates in subgroups were termed well calibrated. A model in which MDM2, DUSP6, CPEB4, MMD, and EIF2S3 were selected showed the result in logistic regression analysis (H-L P = 0.460, R2= 0.853, AUC = 0.978, accuracy = 0.949, specificity = 0.818 and sensitivity = 0.971).A novel gene expression profile was associated with CRC and can potentially be applied to blood-based detection assays.
- Published
- 2014
30. C9orf72 arginine-rich dipeptide proteins interact with ribosomal proteins in vivo to induce a toxic translational arrest that is rescued by eIF1A.
- Author
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Moens, Thomas G., Niccoli, Teresa, Wilson, Katherine M., Atilano, Magda L., Birsa, Nicol, Gittings, Lauren M., Holbling, Benedikt V., Dyson, Miranda C., Thoeng, Annora, Neeves, Jacob, Glaria, Idoia, Yu, Lu, Bussmann, Julia, Storkebaum, Erik, Pardo, Mercedes, Choudhary, Jyoti S., Fratta, Pietro, Partridge, Linda, and Isaacs, Adrian M.
- Subjects
GENE expression ,DROSOPHILA melanogaster - Abstract
A GGGGCC hexanucleotide repeat expansion within the C9orf72 gene is the most common genetic cause of both amyotrophic lateral sclerosis and frontotemporal dementia. Sense and antisense repeat-containing transcripts undergo repeat-associated non-AUG-initiated translation to produce five dipeptide proteins (DPRs). The polyGR and polyPR DPRs are extremely toxic when expressed in Drosophila neurons. To determine the mechanism that mediates this toxicity, we purified DPRs from the Drosophila brain and used mass spectrometry to identify the in vivo neuronal DPR interactome. PolyGR and polyPR interact with ribosomal proteins, and inhibit translation in both human iPSC-derived motor neurons, and adult Drosophila neurons. We next performed a screen of 81 translation-associated proteins in GGGGCC repeat-expressing Drosophila to determine whether this translational repression can be overcome and if this impacts neurodegeneration. Expression of the translation initiation factor eIF1A uniquely rescued DPR-induced toxicity in vivo, indicating that restoring translation is a potential therapeutic strategy. These data directly implicate translational repression in C9orf72 repeat-induced neurodegeneration and identify eIF1A as a novel modifier of C9orf72 repeat toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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31. Shuangyu Tiaozhi Granule Attenuates Hypercholesterolemia through the Reduction of Cholesterol Synthesis in Rat Fed a High Cholesterol Diet.
- Author
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Shi, Jingjing, Li, Ruoqi, Liu, Yi, Lu, Haifei, Yu, Lu, and Zhang, Fengxia
- Subjects
CHOLESTEROL metabolism ,SIMVASTATIN ,ACETYLTRANSFERASES ,ANIMAL experimentation ,CELL receptors ,CELLULAR signal transduction ,CHOLESTEROL ,DIETARY supplements ,DOSE-effect relationship in pharmacology ,FAT content of food ,GENE expression ,HERBAL medicine ,HYPERCHOLESTEREMIA ,LIPIDS ,LIVER ,CHINESE medicine ,MESSENGER RNA ,OXIDOREDUCTASES ,RATS ,TRANSCRIPTION factors ,WEIGHT gain ,CYTOCHROME P-450 ,DRUG administration ,DRUG dosage ,THERAPEUTICS - Abstract
Shuangyu Tiaozhi Granule (STG) is composed of two kinds of Chinese medicinal herbs in dioscorea, which are used for managing cholesterol levels in patients with hypercholesterolemia in traditional Chinese medicine (TCM). However, the potential molecular mechanisms of administration of STG in hypercholesterolemia remain unknown. In this study, we investigated the effects of STG on hepatic cholesterol metabolism in high cholesterol (HC) diet-induced hypercholesterolemic rat models and simvastatin was used as a positive control. Male Sprague Dawley (SD) rats were fed general or HC diet, respectively. After 4 weeks of feeding, HC diet-induced hypercholesterolemic rats were fed HC diet, STG at 5% (w/w) or 10% (w/w) mixed in the HC diet, or HC diet combined with simvastatin gavages (4 mg·kg
−1 ·d−1 ) for 4 or 8 weeks. STG treatment decreased body weight gain, liver weight ratio, serum lipids levels and hepatic lipids accumulation in rats fed a HC diet. Moreover, the effects of STG on decreasing body weight and lowering liver cholesterol levels were in dose- and time-dependent. Furthermore, STG or simvastatin treatment decreased the mRNA and protein levels of HMGCR and SREBP-2 in liver. The ACAT-2 and CYP7A1 mRNA expression were significantly decreased in HC diet supplemented with STG, while the mRNA levels of LDLR were markedly increased. STG attenuates hypercholesterolemia via inhibiting SREBP-2 signaling pathway activation and increasing hepatic uptake genes expression, providing a novel idea of TCM keeping cholesterol levels down for the clinical application. [ABSTRACT FROM AUTHOR]- Published
- 2019
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32. Highly efficient expression of interleukin-2 under the control of rabbit β-globin intron II gene enhances protective immune responses of porcine reproductive and respiratory syndrome (PRRS) DNA vaccine in pigs
- Author
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Feng Li, Lei Chen, Yu Lu, Xinglong Wang, Shi Jianli, Jiyu Liu, Jing Qi, Guo Lihui, Du Yijun, Liu Junzhen, Jun Li, Renzhong Wan, Jiaqiang Wu, Huang Baohua, Yue Hu, Xiaoyan Cong, Haiying Tao, Wenbo Sun, Wang Jinbao, and Ren Sufang
- Subjects
Swine ,animal diseases ,Veterinary Microbiology ,Gene Expression ,beta-Globins ,Antibodies, Viral ,Vaccines, DNA ,Lymphocytes ,Immunity, Cellular ,Mice, Inbred BALB C ,Vaccines ,Multidisciplinary ,Attenuated vaccine ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Viral Vaccine ,Vaccination ,Veterinary Diseases ,Cytokines ,Medicine ,Female ,Rabbits ,Antibody ,Research Article ,Veterinary Medicine ,Animal Types ,Science ,Blotting, Western ,Genetic Vectors ,Porcine Reproductive and Respiratory Syndrome ,Large Animals ,Microbiology ,Veterinary Immunology ,DNA vaccination ,Viral Proteins ,Immune system ,Immunity ,Virology ,Vaccine Development ,Animals ,Humans ,Porcine respiratory and reproductive syndrome virus ,Biology ,Cell Proliferation ,Glycoproteins ,Viral Vaccines ,Veterinary Virology ,Porcine reproductive and respiratory syndrome virus ,biology.organism_classification ,Introns ,Immunity, Humoral ,HEK293 Cells ,Inactivated vaccine ,biology.protein ,Interleukin-2 ,Clinical Immunology ,Veterinary Science - Abstract
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) had caused catastrophic losses in swine industry in China. The current inactivated vaccine provided only limited protection, and the attenuated live vaccine could protect piglets against the HP-PRRSV but there was a possibility that the attenuated virus returned to high virulence. In this study, the eukaryotic expression vector pVAX1© was modified under the control of rabbit β-globin intron II gene and the modified vector pMVAX1© was constructed. Porcine interleukin-2 (IL-2) and GP3-GP5 fusion protein of HP-PRRSV strain SD-JN were highly expressed by pMVAX1©. Mice inoculated with pMVAX1©-GP35 developed significantly higher PRRSV-specific antibody responses and T cell proliferation than those vaccinated with pVAX1©-GP35. pMVAX1©-GP35 was selected as PRRS DNA vaccine candidate and co-administrated with pVAX1©-IL-2 or pMVAX1©-IL-2 in pigs. pMVAX1©-IL-2+pMVAX1©-GP35 could provide enhanced PRRSV-specific antibody responses, T cell proliferation, Th1-type and Th2-type cytokine responses and CTL responses than pMVAX1©-GP35 and pVAX1©-IL-2+pMVAX1©-GP35. Following homologous challenge with HP-PRRSV strain SD-JN, similar with attenuated PRRS vaccine group, pigs inoculated with pMVAX1©-IL-2+pMVAX1©-GP35 showed no clinical signs, almost no lung lesions and no viremia, as compared to those in pMVAX1©-GP35 and pVAX1©-IL-2+pMVAX1©-GP35 groups. It indicated that pMVAX1©-IL-2 effectively increases humoral and cell mediated immune responses of pMVAX1©-GP35. Co-administration of pMVAX1©-IL-2 and pMVAX1©-GP35 might be attractive candidate vaccines for preventing HP-PRRSV infections.
- Published
- 2014
33. Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice.
- Author
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Oshota, Olusegun, Conway, Max, Fookes, Maria, Schreiber, Fernanda, Chaudhuri, Roy R., Yu, Lu, Morgan, Fiona J. E., Clare, Simon, Choudhary, Jyoti, Thomson, Nicholas R., Lio, Pietro, Maskell, Duncan J., Mastroeni, Pietro, and Grant, Andrew J.
- Subjects
SALMONELLA enterica ,PUBLIC health ,PHAGOCYTES ,CELL division ,MEDICAL microbiology ,BACTERIA - Abstract
Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S. enterica adapts in vivo to sustain cell division and survival. We have studied the transcriptome, proteome and metabolic flux of Salmonella, and the transcriptome of the host during infection of wild type C57BL/6 and immune-deficient gp91
-/- phox mice. Our analyses advance the understanding of how S. enterica and the host behaves during infection to a more sophisticated level than has previously been reported. [ABSTRACT FROM AUTHOR]- Published
- 2017
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34. Dynamical Regulation Analysis Identifies Molecular Mechanisms of Fuzheng-Huayu Formula against Hepatitis B-Caused Liver Cirrhosis.
- Author
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Qi-Long Chen, Yi-Yu Lu, Jing-Hua Peng, Shu Dong, Bin Wei, Ya-Nan Song, Qian-Mei Zhou, Hui Zhang, Ping Liu, and Shi-Bing Su
- Subjects
- *
BIOMARKERS , *CLINICAL trials , *CLUSTER analysis (Statistics) , *GENE expression , *HEPATITIS B , *HERBAL medicine , *CIRRHOSIS of the liver , *MATHEMATICAL statistics , *CHINESE medicine , *PLACEBOS , *RNA , *STATISTICS , *T-test (Statistics) , *PARAMETERS (Statistics) , *CONTROL groups , *MICROARRAY technology , *DISEASE complications - Abstract
Fuzheng-Huayu (FZHY) tablet was formulated based on Chinese medicine theory in treating liver fibrosis. A clinical trial has indicated that FZHY can against hepatitis B-caused liver cirrhosis (HBC), but the underlying mechanism of FZHY efficacy is unclear. Here, we report that miRNA expression levels are remarkably changed when FZHY formula was used in HBC patient's treatment as a paradigm of trials. Then, we functionally characterize the significant impact of potential kernel miRNAs by miRNA-target network analysis. Enrichment analysis show that the FZHY formula dramatically effecting the molecular regulated module in HBC. Thus, we infer that FZHY plays a critical function in HBC treatment process and directly regulated many important pathways, including but not limited to cell cycle, p53 signaling pathway, and TGF-β signaling pathway, suggesting a new strategy for investigating the molecular mechanism of FZHY treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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35. Galectin-3 – A jack-of-all-trades in cancer
- Author
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Newlaczyl, Anna U. and Yu, Lu-Gang
- Subjects
- *
TUMOR markers , *CARRIER proteins , *GENE expression , *CANCER cell growth , *CARCINOGENESIS , *METASTASIS , *GLYCOSIDES - Abstract
Abstract: Galectin-3 is a mammalian β-galactoside-binding protein that is expressed by various types of human cells. Changes in galectin-3 expression and subcellular and intercellular localizations are commonly seen in cancer and pre-cancerous conditions. It is increasingly recognized that galectin-3 is an important regulator of a broad range of cancer cell activities and plays important roles in cancer cell growth, transformation, apoptosis, angiogenesis, adhesion, invasion and metastasis. Such a divergent influence of galectin-3 on cancer cell activities derives from its multiple inter- and sub-cellular localizations where it interacts with a range of different binding partners. This mini-review summaries the diverse influences of galectin-3 on cancer cell behaviours with particular emphasis on its role in tumorigenesis and metastasis. [Copyright &y& Elsevier]
- Published
- 2011
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36. Down-regulation of connexin43 gap junction by serum deprivation in human endothelial cells was improved by (−)-Epigallocatechin gallate via ERK MAP kinase pathway
- Author
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Zhao, Yanbo, Yu, Lu, Xu, Shengjie, Qiu, Fuyu, Fan, Youqi, and Fu, Guosheng
- Subjects
- *
CONNEXINS , *GAP junctions (Cell biology) , *SERUM , *ENDOTHELIUM , *MITOGEN-activated protein kinases , *EPIGALLOCATECHIN gallate , *CELLULAR signal transduction , *GENE expression - Abstract
Abstract: Intercellular communication through gap junctions (GJIC) plays an essential role in maintaining the functional integrity of vascular endothelium. Despite emerging evidence suggests that (−)-Epigallocatechin gallate (EGCG) may improve endothelial function. However, its effect on Cx43 gap junction in endothelial cells remains unexplored. Here we investigated the effect of EGCG on connexin43 (Cx43) gap junction in endothelial cells. The levels of Cx43 protein in human umbilical vein endothelial cells (HUVECs) cultured under serum-deprivation 48h decreased about 50%, accompanied by decreased GJIC. This reduction can be reversed by treatments with EGCG. In addition, EGCG activated ERK, P38, and JNK mitogen-activated protein kinases (MAPKs), which were supposed to participate in the regulation of Cx43. A MEK inhibitor PD98059, but not SB203580 (a p38 kinase inhibitor) or SP600125 (a JNK kinase inhibitor), abolished the effects of EGCG on Cx43 expression and GJIC. Moreover, although both Akt and eNOS phosphorylation were time-dependently augmented by EGCG, neither PI3K inhibitor LY294002 nor eNOS inhibitor L-NAME blocked the effects of EGCG on Cx43 gap junctions. Thus, EGCG attenuated Cx43 down-regulation and impaired GJIC induced by serum deprivation, ERK MAPK Signal transduction pathway appears to be involved in these processes. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
37. Effects of small interfering RNAs targeting Fascin on gene expression in oral cancer cells.
- Author
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Chen, Su‐Feng, Lin, Chi‐Yu, Chang, Yun‐Ching, Li, Jhy‐Wei, Fu, Earl, Chang, Fei‐Ni, Lin, Yu‐Lu, and Nieh, Shin
- Subjects
TREATMENT of oral cancer ,CANCER cells ,RIBONUCLEASES ,RNA ,GENE expression ,CANCER prognosis - Abstract
Background: Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers. The prognosis of OSCC is usually poor because of extensive local invasion at initial diagnosis. In the literature, Fascin has been reported responsible for cell motility and over-expression of Fascin contributes to an unfavorable clinical course. Nevertheless, the roles of Fascin protein playing in aggressiveness of OSCC and their potential mechanisms need to be elucidated. Methods: Two cell lines of OSCC (OECM-1 and SCC-25) via the vector-based small interfering RNA (siRNA) to suppress the expression of the Fascin gene were used. Subsequent analyses and observation regarding the expression of Fascin protein and cyto-morphological alterations were detected by Western blot and immunofluorescent microscopy. Boyden chamber invasion assay, cell migration assay and adhesion assay were also applied to investigate the functional changes of OSCC. Results: There were statistically significant differences ( P < 0.05) of Fascin expression before and after silencing. Down-regulation of Fascin protein directly led to changes of cell surface protrusions under immunofluorescent microscopy and resulted in suppression of migration, invasion and increase of adhesion in both cell lines ( P < 0.05). Furthermore, down-regulation of Fascin expression also resulted in alterations of E-cadherin, β-catenin and TWIST at certain level, implicative of an association with epithelial-mesenchymal transition (EMT). Conclusions: Our results suggest that expression of Fascin protein may play an essential role in regulation of progression of OSCC and contributes to the event of EMT in the early aggressiveness of OSCC. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
38. Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment.
- Author
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Hai-Chao Zhang, Jing-Mei Liu, Hong-Yu Lu, and Shan-Lin Gao
- Subjects
FLAVONOIDS ,ROOT hairs (Botany) ,PLANT cell culture ,GLYCYRRHIZA ,ISOMERASES ,GENE expression ,PLANT genetic engineering ,GENETIC transformation - Abstract
Abstract  Economically important compounds, such as licorice flavonoids, are present in insufficient amounts in the hairy roots. To overcome this problem, we took the transgenic approach combined with the elicitation technique to increase the flavonoid production. The Glycyrrhiza uralensis Fisch cDNA encoding chalcone isomerase gene (chi) was over-expressed in hairy roots of G. uralensis Fisch mediated by the disarmed Agrobacterium rhizogenes A4. Stable genetic transformation was confirmed by Southern blot analysis. The transgenic and wild cultures were subsequently elicited with PEG8000 (2%) alone, yeast extract (YE) (0.1%) alone, or both of them, and then the total flavonoids were extracted and measured. The results showed that over a culture period of 3 weeks, the wild-type hairy roots, the untreated transgenic hairy roots, and the double-treated transgenic hairy roots accumulated 0.842, 1.394, and 2.838 (g/100 g DW) of total flavonoids, respectively. Moreover, the enhanced accumulation of flavonoids were correlated with the elevated level of chi transcripts and CHI activity, confirming the key role of chi in the flavonoids synthesis. This research demonstrated that the combination of the metabolic engineering and PEG8000-YE elicitation treatment was an effective strategy to increase the flavonoids production in hairy roots of G. uralensis Fisch. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
39. Aurora A Is Essential for Early Embryonic Development and Tumor Suppression.
- Author
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Lin-Yu Lu, Wood, Jamie L., Lin Ye, Minter-Dykhouse, Katherine, Saunders, Thomas L., Xiaochun Yu, and Junjie Chen
- Subjects
- *
SERINE , *PROTEIN kinases , *GENE amplification , *GENE expression , *ONCOGENES , *TUMOR suppressor genes , *FIBROBLASTS - Abstract
Aurora A is a serine/threonine kinase that functions in various stages of mitosis. Accumulating evidence has demonstrated that gene amplification and overexpression of Aurora A are linked to tumorigenesis, suggesting that Aurora A is an oncogene. In addition, Aurora A overexpression has been used as a negative prognostic marker, because it is associated with resistance to anti-mitotic agents commonly used for cancer therapy. To understand the physiological functions of Aurora A, we generated Aurora A knock-out mice. Aurora A null mice die early during embryonic development before the 16-cell stage. These Aurora A null embryos have defects in mitosis, particularly in spindle assembly, supporting critical functions of Aurora A during mitotic transitions. Interestingly, Aurora A heterozygosity results in a significantly increased tumor incidence in mice, suggesting that Aurora A may also act as a haploinsufficient tumor suppressor. Consistently, Aurora A heterozygous mouse embryonic fibroblasts have higher rates of aneuploidy. We further discovered that VX-680, an Aurora kinase inhibitor currently in phase II clinical trials for cancer treatment, could induce aneuploidy in wild type mouse embryonic fibroblasts. We conclude that a balanced Aurora A level is critical for maintaining genomic stability and one needs to be fully aware of the potential side effects of anti-cancer therapy based on the use of Aurora A-specific inhibitors. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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- View/download PDF
40. cDNA microarray analysis of the expression profiles of Trichophyton rubrum in response to novel synthetic fatty acid synthase inhibitor PHS11A
- Author
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Zhang, Wenliang, Yu, Lu, Leng, Wenchuan, Wang, Xiaokui, Wang, Lingling, Deng, Xuming, Yang, Jian, Liu, Tao, Peng, Junping, Wang, Jin, Li, Song, and Jin, Qi
- Subjects
- *
TRICHOPHYTON , *FATTY acids , *GENE expression , *DNA microarrays - Abstract
Abstract: Trichophyton rubrum (T. rubrum) is a major pathogen responsible for dermatophytosis. Because of potential relapse of disease with current antifungal therapy protocols, there is a need for additional and/or alternative antifungal agents for the treatment of disease caused by T. rubrum. We synthesized a potent fungal fatty acid synthase inhibitor, PHS11A, based on the structure of fungal fatty acid synthase. The antifungal activities of PHS11A were tested against 38 clinical isolates of T. rubrum and compared with those of ketoconazole and terbinafine, the MIC50 and MIC90 of PHS11A on the isolates were 2 and 4μg/ml, respectively. We evaluated the transcriptional response of T. rubrum hyphae exposed to PHS11A using 11,232-spot cDNA microarrays. PHS11A exposure increased transcription of fatty acid synthases (FASs) genes FAS1 and FAS2. PHS11A also affected transcription of some genes involved in lipid metabolism, cAMP and MAPK pathways, and multidrug resistance. Quantitative real-time PCR was performed for selected genes to verify the microarray results. [Copyright &y& Elsevier]
- Published
- 2007
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- View/download PDF
41. Temperature dependence of human ether-à-go-go-related gene K+ currents.
- Author
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Vandenberg, Jarnie I., Varghese, Anthony, Yu Lu, Bursill, Jane A., Mahaut-Smith, Martyn P., and Huang, Christopher L.-H.
- Subjects
PHYSIOLOGICAL research ,POTASSIUM channels ,THERMODYNAMICS ,GENE expression ,CELL lines ,OVARIES - Abstract
The function of voltage-gated human ether-a-go-go related gene (hERG) K
+ channels is critical for both normal cardiac repolarization and suppression of arrhythmias initiated by premature excitation. These important functions are facilitated by their unusual kinetics that combine relatively slow activation and deactivation with rapid and voltage-dependent inactivation and recovery from inactivation. The thermodynamics of these unusual features were examined by exploring the effect of temperature on the activation and inactivation processes of hERG channels expressed in Chinese hamster ovary cells, Increased temperature shifted the voltage dependence of activation in the hyperpolarizing direction but that of inactivation in the depolarizing direction. This increases the relative occupancy of the open state and contributes to the marked temperature sensitivity of hERO current magnitude observed during action potential voltage clamps. The rates of activation and deactivation also increase with higher temperatures, but less markedly than do the rates of inactivation and recovery from inactivation. Our results also emphasize that one cannot extrapolate results obtained at room temperature to 37°C by using a single temperature scale factor. [ABSTRACT FROM AUTHOR]- Published
- 2006
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- View/download PDF
42. <italic>N</italic><italic>6</italic>-methyl-adenosine level in <italic>Nicotiana tabacum</italic> is associated with tobacco mosaic virus.
- Author
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Li, Zhurui, Shi, Jing, Yu, Lu, Zhao, Xiaozhen, Ran, Longlu, Hu, Deyu, and Song, Baoan
- Subjects
TOBACCO mosaic virus ,RNA modification & restriction ,METHYLTRANSFERASES ,POLYMERASE chain reaction ,GENE expression ,HIGH performance liquid chromatography ,TANDEM mass spectrometry - Abstract
Background:
N -methyl-adenosine (m6 6 A) is a prevalent RNA modification in many species. Abnormal m6 A methylation levels can lead to RNA dysfunction and can cause diseases. Tobacco mosaic virus (TMV) is one of the most devastating viruses for agricultural plants. It has many hosts, particularly including tobacco and other members the family Solanaceae. However, it remains unclear whether the abnormal growth induced by TMV is associated with the m6 A level. Methods: A rapid and accurate analytical method using ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC−HR − MS/MS) was developed to analyse the adenosine (A), cytidine (C), guanosine (G), uridine (U), and m6 A contents in the tobacco leaf, and the m6 A/G ratio was used to evaluate the m6 A level. Subsequent protein sequence alignments were used to find the potential methylases and demethylases inNicotiana tabacum (N. tabacum ). Finally, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was used to analyse the gene expression levels of the potential methylases and demethylases in theN. tabacum leaf. Results: The results showed that TMV reduced the m6 A level. Moreover, protein sequence alignments revealed partial homology among human ALKBH5,Arabidopsis (NP_001031793), andNicotiana sylvestris (XP_009800010). The gene expression level of the potential demethylase XM_009801708 increased at 14 and 21 days inN. tabacum infected with TMV, whereas all of the potential methylases decreased. Conclusions: The reversible m6 A modification inN. tabacum mRNA might represent a novel epigenetic mechanism involved in TMV. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
43. Double-strand RNAs targeting MaltRelish and MaltSpz reveals potential targets for pest management of Monochamus alternatus.
- Author
-
Chu, Xu, Yang, Meijiao, Yu, Lu, Xie, Hongyun, Liu, Jinyan, Wu, Songqing, Zhang, Feiping, and Hu, Xia
- Subjects
- *
PINEWOOD nematode , *ENTOMOPATHOGENIC fungi , *GENE expression , *PEST control , *INSECT nematodes , *INSECT genes - Abstract
Overcoming the innate immunity of insects is a key process to improve the efficiency of biological control. Antimicrobial peptides (AMPs) are important effectors in insect innate immunity, usually mediating resistance to pathogenic microorganisms through Toll and IMD signaling pathways. This study investigated the effect of key genes on upstream immune recognition receptor (GNBP 3) and downstream effectors (AMPs) by RNAi technology. The transcriptome KEGG enrichment analysis and differential gene annotation results showed that the immune response genes MaltSpz and MaltRelish are important regulators of Toll and IMD signaling pathways, respectively. Both ds Spz and ds Relish could affect AMP gene expression and increase the expression of the immune recognition receptor MaltGNBP 3. Moreover, they significantly reduce the survival rate of Monochamus alternatus and promote hyphal growth after Beauveria bassiana infection. This helps to improve the biological control effect of B. bassiana , control the population of vector insects and cut off the transmission route of pine wood nematode. The combined MaltSpz and MaltRelish knockdown increased the infection rate of M. alternatus larvae from 20.69% to 83.93%, achieving the best efficiency in synergistic B. bassiana infection. Our results showed important roles of MaltRelish- and MaltSpz -mediated regulation of AMP genes function in insect entomopathogenic fungi tolerance and induced significant mortality in larvae. Based on this study, MaltSpz and MaltRelish could represent candidate gene targets for the biological control of M. alternatus by RNAi. Insect innate immunity is a key process to resist the pathogenic microorganisms. After pathogenic microorganisms and RNAi interfered with the immune genes (Relish,Spz) of Monochamus alternatus larvae, the biosynthesis pathway of antimicrobial peptides in Toll and IMD signaling pathways was significantly inhibited, which increased the infection rate of pathogenic fungi. [Display omitted] • Monochamus alternatus AMPs regulating insect tolerance to pathogenic fungi. • RNAi-mediated knockdown of MaltRelish and MaltSpz affected the expression of AMPs. • Combined of B. bassiana and RNAi improved the control efficiency of M. alternatus. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
44. Transcriptomic and proteomic analyses reveal new insights into the regulation of immune pathways during cyprinid herpesvirus 2 infection in vitro.
- Author
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Fei, Yueyue, Han, Minzhen, Chu, Xin, Feng, Zizhao, Yu, Lu, Luo, Yang, Lu, Liqun, and Xu, Dan
- Subjects
- *
HERPESVIRUS diseases , *PROTEOMICS , *GOLDFISH , *GENE expression profiling , *GENE expression - Abstract
Carassius auratus gibelio is susceptible to the herpesviral hematopoietic necrosis (HVHN) disease caused by cyprinid herpesvirus 2 (CyHV-2) infection during the breeding process. Nevertheless, the report on biological response of CyHV-2 with C. auratus gibelio was limited, especially in vitro. In this study, host gene expression profiling was mostly analyzed in caudal fin cells of Carassius auratus gibelio (GiCF) underlying CyHV-2 infection. Transcriptomics and proteomics were employed to study the differential expression gene and revealed the host genes involved in pathway during the CyHV-2 infection. Transcriptome analysis revealed that compared with the control group, there were 11 335 and 19 421 differentially expressed unigenes at 48 h and at 96 h, respectively. Furthermore, proteome analysis showed that there were a total of 9008 proteins, among which 169 proteins were differential expression in the 48 h group and 502 proteins in the 96 h group. Notably, 10 and 158 differentially co-expressed genes at mRNA and protein levels (cDEGs) were reliably quantified at 48 h and 96 h, respectively. Interestingly, significantly different expressed genes both in the transcriptome and the proteome were identified, including GNG7, Hsp90a, THBS1 and RRM2. The result suggested that PI3k-AKT pathway was activated, but the p53 signaling pathway was suppressed. The above result will lay the foundation for understanding the mechanisms of host defense virus invasion during CyHV-2 infection. • 31 and 167 differentially co-expressed genes were identified at 48 h and at 96 h inCyHV-2infected GiCF cell, respectively. • 10 differentially co-expressed genes both at 48 h and 96 h were verified by Real-time PCR in CyHV-2 infected GiCF cell. • PI3K-AKT pathway and p53 signal pathway play their important roles in GiCF cell during CyHV-2 infection. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
45. Analysis of the gene expression profile of Staphylococcus aureus treated with nisin.
- Author
-
Zhao, Xingchen, Meng, Rizeng, Shi, Ce, Liu, Zonghui, Huang, Yanjun, Zhao, Ziwen, Guo, Na, and Yu, Lu
- Subjects
- *
FOOD industry , *FOOD poisoning , *STAPHYLOCOCCUS aureus , *GENE expression , *NISIN , *DNA microarrays - Abstract
The bacterium Staphylococcus aureus is most often recognized as a common cause of food poisoning. S. aureus colonization in humans can cause serious infections, toxinoses and life-threatening diseases. Nisin is a bacteriocin that has been extensively used as a natural preservative in the food industry, but the overall transcriptional response of S. aureus to nisin has not been well characterized. We used whole-genome DNA microarrays of S. aureus to determine the global transcription profile triggered by nisin treatment in S. aureus . In response to nisin treatment, a total of 601 genes were differentially regulated; transcription of 327 genes was up-regulated, and transcription of 274 genes was down-regulated. Various transporter genes showed elevated transcription, including genes involved in capsule polysaccharide (CP) synthesis, cell-wall synthesis, nucleic-acid and nucleotide metabolism as well as genes encoding urease, transport/binding proteins, and lipoproteins. To our knowledge, this transcriptome analysis provides the first insights into the transcriptional response of S. aureus to nisin challenge and enables exploration of the mechanisms of nisin antimicrobial activity toward S. aureus . The findings suggest that nisin regulates multiple desirable targets which could be further explored in the development of new food preservatives to avoid the contamination of S. aureus in food processing and storage. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
46. NF-kappaB p65 modulates the telomerase reverse transcriptase in the HepG2 hepatoma cell line
- Author
-
Zuo, Qing-Ping, Liu, Shi-Kun, Li, Zuo-Jun, Li, Bing, Zhou, Yu-Lu, Guo, Ren, and Huang, Li-Hua
- Subjects
- *
NF-kappa B , *TELOMERASE , *LIVER cancer , *GENE expression , *CARCINOGENESIS , *GENETIC transcription , *TRANSCRIPTION factors - Abstract
Abstract: Nuclear factor-kappa B (NF-kappaB) regulates the expression of various genes, several genes involved in inflammation and tumorigenesis, including those of the liver. A role for NF-kappaB has been implicated in the pathogenesis of hepatocellular carcinoma. This transcription factor can regulate hTERT gene transcription. Expression of hTERT was found to be at high levels in hepatocellular carcinoma. However, positive effects of NF-kappaB on hTERT protein synthesis in HepG2 cells are unknown. In this study, we show that LPS (specific binding to TLR4 to activate NF-kappaB) was positive for NF-kappaB p65 mRNA expression and activation, and also up-regulated hTERT mRNA and protein expressions at 36h in a dose-dependent manner. In contrast, MG-132 (blocking the activity of 26S proteasome and thereby preventing nuclear translocation of NF-kappaB) significantly inhibited activation of NF-kappaB and mRNA expression. And also reduced the expression of hTERT at both mRNA and protein levels at 36h in a dose-dependent manner. Furthermore, dexamethasone inhibited LPS-induced activation of NF-kappaB and expression of the hTERT in HepG2 cells. These findings suggest that NF-kappaB may modulate hTERT mRNA level, importantly, in protein level in HepG2 cells and dexamethasone inhibits LPS-induced hTERT via blocking NF-kappaB. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
47. Cell Death Caused by Single-Stranded Oligodeoxynucleotide-Mediated Targeted Genomic Sequence Modification.
- Author
-
Chenli Liu, Zai Wang, Michael S.Y. Huen, Lin-Yu Lu, De-Pei Liu, and Jian-Dong Huang
- Subjects
- *
CELL death , *BIOTECHNOLOGY , *GENE therapy , *MMR vaccines , *GENETIC toxicology , *GENE expression - Abstract
Targeted gene repair directed by single-stranded oligodeoxynucleotides (ssODNs) offers a promising tool for biotechnology and gene therapy. However, the methodology is currently limited by its low frequency of repair events, variability, and low viability of “corrected” cells. In this study, we showed that during ssODN-mediated gene repair reaction, a significant population of corrected cells failed to divide, and were much more prone to undergo apoptosis, as marked by processing of caspases and PARP-1. In addition, we found that apoptotic cell death triggered by ssODN-mediated gene repair was largely independent of the ATM/ATR kinase. Furthermore, we examined the potential involvement of the mismatch repair (MMR) proteins in this “correction reaction-induced” cell death. Result showed that while defective MMR greatly enhanced the efficiency of gene correction, compromising the MMR system did not yield any viable corrected clone, indicating that the MMR machinery, although plays a critical role in determining ssODN-directed repair, was not involved in the observed cellular genotoxic responses. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
48. Valnemulin downregulates nitric oxide, prostaglandin E2, and cytokine production via inhibition of NF-κB and MAPK activity
- Author
-
Zhang, Xuemei, Li, Hongyu, Feng, Haihua, Xiong, Huanzhang, Zhang, Lei, Song, Yu, Yu, Lu, and Deng, Xuming
- Subjects
- *
ANTIBIOTICS , *NITRIC oxide , *PROSTAGLANDINS , *NF-kappa B , *MITOGEN-activated protein kinases , *CYTOKINES , *CELLULAR signal transduction , *CYCLOOXYGENASE 2 inhibitors , *GENE expression , *NITRIC-oxide synthases - Abstract
Abstract: Valnemulin is a pleuromutilin antibiotic used in clinics for the treatment of various infections. We studied the in vitro anti-inflammatory effects of valnemulin and associated signal transduction mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We found that valnemulin inhibited nitric oxide (NO), prostaglandin E2 (PGE2), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and increased interleukin-10 (IL-10) production. Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression were also inhibited by valnemulin. We further observed that valnemulin prevented the LPS-induced NF-κB translocation from the cytoplasm into the nucleus. Valnemulin also blocked phosphorylation of three mitogen-activated protein kinases (MAPKs): extracellular signal receptor-activated kinase (ERK) 1/2, p38, and c-Jun N-terminal kinase (JNK). Our data indicate that valnemulin may have therapeutic anti-inflammatory effects independent of its antibacterial activity. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
49. Polyphenols isolated from lychee seed inhibit Alzheimer's disease-associated Tau through improving insulin resistance via the IRS-1/PI3K/Akt/GSK-3β pathway.
- Author
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Xiong, Rui, Wang, Xiu-Ling, Wu, Jian-Ming, Tang, Yong, Qiu, Wen-Qiao, Shen, Xin, Teng, Jin-Feng, Pan, Rong, Zhao, Ya, Yu, Lu, Liu, Jian, Chen, Hai-Xia, Qin, Da-Lian, Yu, Chong-Lin, and Wu, An-Guo
- Subjects
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ALZHEIMER'S disease , *CELLULAR signal transduction , *CHROMATOGRAPHIC analysis , *EPITHELIAL cells , *FLUORESCENT antibody technique , *GENE expression , *HERBAL medicine , *INSULIN , *CHINESE medicine , *MESSENGER RNA , *NERVE tissue proteins , *PHOSPHORYLATION , *POLYMERASE chain reaction , *POLYPHENOLS , *PROTEIN kinases , *SEEDS , *WESTERN immunoblotting , *DEXAMETHASONE , *SIGNAL peptides - Abstract
Lychee seed, the seed of Litchi chinensis Sonn. is one of the commonly used in traditional Chinese medicine (TCM). It possesses many pharmacological effects such as blood glucose and lipid-lowering effects, liver protection, and antioxidation. Our preliminary studies have proven that an active fraction derived from lychee seed (LSF) can significantly decrease the blood glucose level, inhibit amyloid-β (Aβ) fibril formation and Tau hyperphosphorylation, and improve the cognitive function and behavior of Alzheimer's disease (AD) model rats. The aim of this study was to identify the main active components in LSF that can inhibit the hyperphosphorylation of Tau through improving insulin resistance (IR) in dexamethasone (DXM)-induced HepG2 and HT22 cells. The isolation was guided by the bioactivity evaluation of the improvement effect of IR in HepG2 and HT22 cells. The mRNA and protein expressions of IRS-1, PI3K, Akt, GSK-3β, and Tau were measured by RT-PCR, Western blotting, and immunofluorescence methods, respectively. After extraction, isolation, and elucidation using chromatography and spectrum technologies, three polyphenols including catechin, procyanidin A1 and procyanidin A2 were identified from fractions 3, 5, and 9 derived from LSF. These polyphenols inhibit hyperphosphorylated Tau via the up-regulation of IRS-1/PI3K/Akt and down-regulation of GSK-3β. Molecular docking result further demonstrate that these polyphenols exhibit good binding property with insulin receptor. catechin, procyanidin A1, and procyanidin A2 are the main components in LSF that inhibit Tau hyperphosphorylation through improving IR via the IRS-1/PI3K/Akt/GSK-3β pathway. Therefore, the findings in the current study provide novel insight into the anti-AD mechanism of the components in LSF derived from lychee seed, which is valuable for the further development of a novel drug or nutrient supplement for the prevention and treatment of AD. Image 1 [ABSTRACT FROM AUTHOR]
- Published
- 2020
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